MD02G1187400.v1.1

NEMP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0001753
Physical Location & Seq
Reverse (-)
17072049 .. 17074363
2315 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1187400.v1.1.491

Sequence Viewer

Length: 717 bp
ATGGCTTCTGCACATTCGGTTCGGATCTTCGCCTGCATTCCCTGGCTTCTCTTCCTCTCACTCCCTTTTTCCTCCTCCGTCGAACCCTCCATTGAGCTTTCTTTAGTTATTTCCGAGTCGGATAGGTTACAATTATCTCTTGGGGTGCCAGTAAAAAAATTTCCCGGTTCCAAGCCCGGCATTTTGGTGCTGCTTAAAAATATTGGGAAGTTTGCCAACACGGTGAAGGTGAAGGTATCAGGAACAAATTCAAGCACTCGTATACCGACCTTCGAGAAATACATCTCTTGGGATAGGGATGTGCCCTCACAGCTCTTGGCAGAAGGTTCCCAAGGGCTCTGGTCAAGGTCCATGTCACCTTTTGAGCACCAACTAATAGATGTACGGACATATGGTTCATCCTTAGAGAGCTTGGAGGTGTCCATTGAAGAAGAATTTGTTAAATATCACGTAACATTTTTGATATTGGGCATAATCATGATGAGCTTGGCGTCCCACCTGAGCAAGTCATTAGTATTTTATTACGGCAGTGGAATGGCAAATTGGGGTAGTCCTTGTAATATTGATGGTACTTTTTCAGGGGATGAAGCTTCTCCCAACTGGTCGGAAGAATTCTCTTGCAATTTTTGTGTACTCATCTGTGGTTGGTATGGGATCTTTTCTCCTCGGCTACCTACCTGGGTCATTGCGTACACTACTCACAGAGATTGGAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NEMP

Protein Analysis

239

Amino Acids

26.54

Weight (kDa)

5.95

Isoelectric Point (pI)

53.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NEMP PF10225 139 - 180 1.4e-08 NEMP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 145
AccI GTMKAC 1 cut(s) 262
AclWI GGATC 2 cut(s) 32, 662
AcsI RAATTY 4 cut(s) 158, 247, 434, 611
AcyI GRCGYC 1 cut(s) 491
AfaI GTAC 4 cut(s) 384, 571, 633, 692
AgsI TTSAA 2 cut(s) 252, 428
AjnI CCWGG 2 cut(s) 41, 677
AjuI GAANNNNNNNTTGG 2 cut(s) 526, 558
AloI GAACNNNNNNTCC 2 cut(s) 379, 411
AluBI AGCT 5 cut(s) 97, 313, 411, 486, 590
AluI AGCT 5 cut(s) 97, 313, 411, 486, 590
Alw21I GWGCWC 1 cut(s) 369
AlwI GGATC 2 cut(s) 32, 662
ApeKI GCWGC 1 cut(s) 190
ApoI RAATTY 4 cut(s) 158, 247, 434, 611
Asp700I GAANNNNTTC 1 cut(s) 247
AspS9I GGNCC 1 cut(s) 348
AsuC2I CCSGG 2 cut(s) 165, 177
AsuHPI GGTGA 3 cut(s) 235, 241, 348
AvaII GGWCC 1 cut(s) 348
BaeGI GKGCMC 1 cut(s) 306
BanI GGYRCC 1 cut(s) 145
BanII GRGCYC 1 cut(s) 339
Bbv12I GWGCWC 1 cut(s) 369
BbvI GCAGC 1 cut(s) 177
BccI CCATC 1 cut(s) 560
BceAI ACGGC 1 cut(s) 541
BciT130I CCWGG 2 cut(s) 43, 679
BcnI CCSGG 2 cut(s) 165, 177
BisI GCNGC 1 cut(s) 191
BlsI GCNGC 1 cut(s) 192
Bme1390I CCNGG 4 cut(s) 43, 165, 177, 679
Bme18I GGWCC 1 cut(s) 348
BmgT120I GGNCC 1 cut(s) 348
BmiI GGNNCC 3 cut(s) 147, 169, 328
BmrFI CCNGG 4 cut(s) 43, 165, 177, 679
Bpu10I CCTNAGC 1 cut(s) 500
BpuMI CCSGG 2 cut(s) 165, 177
BsaAI YACGTR 1 cut(s) 451
BsaHI GRCGYC 1 cut(s) 491
BsaJI CCNNGG 4 cut(s) 41, 331, 665, 678
Bse1I ACTGG 2 cut(s) 149, 605
Bse3DI GCAATG 1 cut(s) 684
BseBI CCWGG 2 cut(s) 43, 679
BseDI CCNNGG 4 cut(s) 41, 331, 665, 678
BseGI GGATG 3 cut(s) 304, 398, 589
BseMI GCAATG 1 cut(s) 684
BseMII CTCAG 1 cut(s) 491
BseNI ACTGG 2 cut(s) 149, 605
BseRI GAGGAG 2 cut(s) 64, 654
BseSI GKGCMC 1 cut(s) 306
BseXI GCAGC 1 cut(s) 177
BshNI GGYRCC 1 cut(s) 145
BsiHKAI GWGCWC 1 cut(s) 369
BsiSI CCGG 2 cut(s) 165, 177
BslFI GGGAC 1 cut(s) 478
BsmFI GGGAC 1 cut(s) 478
BsmI GAATGC 1 cut(s) 36
Bsp1286I GDGCHC 3 cut(s) 306, 339, 369
Bsp143I GATC 2 cut(s) 24, 654
BspCNI CTCAG 1 cut(s) 492
BspHI TCATGA 1 cut(s) 477
BspLI GGNNCC 3 cut(s) 147, 169, 328
BspPI GGATC 2 cut(s) 32, 662
BspT107I GGYRCC 1 cut(s) 145
BsrDI GCAATG 1 cut(s) 684
BsrI ACTGG 2 cut(s) 149, 605
BssECI CCNNGG 4 cut(s) 41, 331, 665, 678
BssMI GATC 2 cut(s) 24, 654
BssNAI GTATAC 1 cut(s) 263
BssNI GRCGYC 1 cut(s) 491
BssT1I CCWWGG 1 cut(s) 331
Bst1107I GTATAC 1 cut(s) 263
Bst2UI CCWGG 2 cut(s) 43, 679
Bst4CI ACNGT 1 cut(s) 223
Bst6I CTCTTC 1 cut(s) 56
BstACI GRCGYC 1 cut(s) 491
BstBAI YACGTR 1 cut(s) 451
BstC8I GCNNGC 1 cut(s) 34
BstDEI CTNAG 2 cut(s) 403, 500
BstF5I GGATG 3 cut(s) 304, 398, 589
BstKTI GATC 2 cut(s) 27, 657
BstMBI GATC 2 cut(s) 24, 654
BstMWI GCNNNNNNNGC 1 cut(s) 310
BstNI CCWGG 2 cut(s) 43, 679
BstSCI CCNGG 4 cut(s) 41, 163, 175, 677
BstSLI GKGCMC 1 cut(s) 306
BstV1I GCAGC 1 cut(s) 177
BstX2I RGATCY 2 cut(s) 24, 654
BstYI RGATCY 2 cut(s) 24, 654
BstZ17I GTATAC 1 cut(s) 263
BtsCI GGATG 3 cut(s) 304, 398, 589
BtsI GCAGTG 1 cut(s) 535
BtsIMutI CAGTG 1 cut(s) 535
Cac8I GCNNGC 1 cut(s) 34
CciI TCATGA 1 cut(s) 477
Cfr13I GGNCC 1 cut(s) 348
CseI GACGC 1 cut(s) 480
Csp6I GTAC 4 cut(s) 383, 570, 632, 691
CviAII CATG 2 cut(s) 352, 478
CviQI GTAC 4 cut(s) 383, 570, 632, 691
DdeI CTNAG 2 cut(s) 403, 500
DpnI GATC 2 cut(s) 26, 656
DpnII GATC 2 cut(s) 24, 654
Eam1104I CTCTTC 1 cut(s) 56
EarI CTCTTC 1 cut(s) 56
Eco130I CCWWGG 1 cut(s) 331
Eco24I GRGCYC 1 cut(s) 339
Eco47I GGWCC 1 cut(s) 348
EcoRI GAATTC 1 cut(s) 611
EcoRII CCWGG 2 cut(s) 41, 677
EcoT14I CCWWGG 1 cut(s) 331
EcoT38I GRGCYC 1 cut(s) 339
ErhI CCWWGG 1 cut(s) 331
FaeI CATG 2 cut(s) 355, 481
FaiI YATR 7 cut(s) 263, 353, 391, 393, 473, 479, 651
FaqI GGGAC 1 cut(s) 478
FatI CATG 2 cut(s) 351, 477
FauNDI CATATG 1 cut(s) 391
FblI GTMKAC 1 cut(s) 262
Fnu4HI GCNGC 1 cut(s) 191
FokI GGATG 3 cut(s) 311, 385, 596
FriOI GRGCYC 1 cut(s) 339
Fsp4HI GCNGC 1 cut(s) 191
GluI GCNGC 1 cut(s) 191
HapII CCGG 2 cut(s) 165, 177
HgaI GACGC 1 cut(s) 480
Hin1I GRCGYC 1 cut(s) 491
Hin1II CATG 2 cut(s) 355, 481
HindIII AAGCTT 1 cut(s) 588
HinfI GANTC 1 cut(s) 116
HpaII CCGG 2 cut(s) 165, 177
HphI GGTGA 3 cut(s) 235, 241, 348
Hpy166II GTNNAC 3 cut(s) 263, 632, 693
Hpy188I TCNGA 4 cut(s) 24, 115, 121, 607
Hpy188III TCNNGA 3 cut(s) 240, 274, 478
Hpy8I GTNNAC 3 cut(s) 263, 632, 693
Hpy99I CGWCG 1 cut(s) 83
HpyAV CCTTC 4 cut(s) 220, 226, 280, 317
HpyCH4III ACNGT 1 cut(s) 223
HpyCH4IV ACGT 1 cut(s) 450
HpyCH4V TGCA 3 cut(s) 11, 36, 621
HpyF10VI GCNNNNNNNGC 1 cut(s) 310
HpyF3I CTNAG 2 cut(s) 403, 500
HpySE526I ACGT 1 cut(s) 450
Hsp92I GRCGYC 1 cut(s) 491
Hsp92II CATG 2 cut(s) 355, 481
Kzo9I GATC 2 cut(s) 24, 654
Lsp1109I GCAGC 1 cut(s) 177
MaeII ACGT 1 cut(s) 450
MaeIII GTNAC 3 cut(s) 126, 354, 451
MalI GATC 2 cut(s) 26, 656
MboI GATC 2 cut(s) 24, 654
MboII GAAGA 5 cut(s) 19, 43, 440, 443, 620
MflI RGATCY 2 cut(s) 24, 654
MhlI GDGCHC 3 cut(s) 306, 339, 369
MluCI AATT 8 cut(s) 131, 158, 247, 434, 541, 611, 622, 712
MlyI GAGTC 1 cut(s) 125
MmeI TCCRAC 2 cut(s) 99, 585
MnlI CCTC 7 cut(s) 65, 82, 85, 97, 316, 409, 675
MroXI GAANNNNTTC 1 cut(s) 247
MseI TTAA 2 cut(s) 195, 441
MslI CAYNNNNRTG 2 cut(s) 185, 476
MspI CCGG 2 cut(s) 165, 177
MspR9I CCNGG 4 cut(s) 43, 165, 177, 679
Mva1269I GAATGC 1 cut(s) 36
MvaI CCWGG 2 cut(s) 43, 679
MwoI GCNNNNNNNGC 1 cut(s) 310
NciI CCSGG 2 cut(s) 165, 177
NdeI CATATG 1 cut(s) 391
NdeII GATC 2 cut(s) 24, 654
NlaIII CATG 2 cut(s) 355, 481
NlaIV GGNNCC 3 cut(s) 147, 169, 328
NmeAIII GCCGAG 1 cut(s) 646
NmuCI GTSAC 1 cut(s) 354
PagI TCATGA 1 cut(s) 477
PctI GAATGC 1 cut(s) 36
PdmI GAANNNNTTC 1 cut(s) 247
PkrI GCNGC 1 cut(s) 192
PleI GAGTC 1 cut(s) 124
PpsI GAGTC 1 cut(s) 124
Ppu21I YACGTR 1 cut(s) 451
Psp6I CCWGG 2 cut(s) 41, 677
PspGI CCWGG 2 cut(s) 41, 677
PspN4I GGNNCC 3 cut(s) 147, 169, 328
PspPI GGNCC 1 cut(s) 348
PsuI RGATCY 2 cut(s) 24, 654
RsaI GTAC 4 cut(s) 384, 571, 633, 692
RsaNI GTAC 4 cut(s) 383, 570, 632, 691
RseI CAYNNNNRTG 2 cut(s) 185, 476
SaqAI TTAA 2 cut(s) 195, 441
SatI GCNGC 1 cut(s) 191
Sau3AI GATC 2 cut(s) 24, 654
Sau96I GGNCC 1 cut(s) 348
SchI GAGTC 1 cut(s) 125
ScrFI CCNGG 4 cut(s) 43, 165, 177, 679
SduI GDGCHC 3 cut(s) 306, 339, 369
SinI GGWCC 1 cut(s) 348
SmiMI CAYNNNNRTG 2 cut(s) 185, 476
Sse9I AATT 8 cut(s) 131, 158, 247, 434, 541, 611, 622, 712
SspI AATATT 2 cut(s) 202, 562
StyD4I CCNGG 4 cut(s) 41, 163, 175, 677
StyI CCWWGG 1 cut(s) 331
TaaI ACNGT 1 cut(s) 223
TaiI ACGT 1 cut(s) 453
TaqI TCGA 2 cut(s) 81, 273
TasI AATT 8 cut(s) 131, 158, 247, 434, 541, 611, 622, 712
TatI WGTACW 1 cut(s) 631
Tru1I TTAA 2 cut(s) 195, 441
Tru9I TTAA 2 cut(s) 195, 441
TscAI CASTG 1 cut(s) 535
TseFI GTSAC 1 cut(s) 354
TseI GCWGC 1 cut(s) 190
Tsp45I GTSAC 1 cut(s) 354
TspDTI ATGAA 2 cut(s) 387, 600
TspGWI ACGGA 2 cut(s) 67, 400
TspRI CASTG 1 cut(s) 535
VpaK11BI GGWCC 1 cut(s) 348
XapI RAATTY 4 cut(s) 158, 247, 434, 611
XmiI GTMKAC 1 cut(s) 262
XmnI GAANNNNTTC 1 cut(s) 247
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.