MD15G1298300.v1.1

NEMP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0001753
Physical Location & Seq
Reverse (-)
28117564 .. 28122222
4659 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1298300.v1.1.491

Sequence Viewer

Length: 1449 bp
ATGGCTTCTGCAAATTCGGGTCGGATCTTCGCCTGCGTTCTCTGGCTTTTTTTTCTCTCGCTCCCGCTTTCCTCCGCCGTCGAACCCTCCAATGAGCATTCCTTAGTTATTTCCGAGTCGGATAGGTTACAATTATCTCGTGGCGCGCCAGTAACCAATTCTCCTGGCTCCAAGCCTGGCATTTCAGTGCTGTGTGAAAGAGTTCATATCCGTGGATTGTCAAGGCTTAAAAATATTGGGAAGTTTGCCAACACGGTGAAGGTGAAGGTATCAGGAACAAATTCAAGCACTCGTATACCGACCTTTGAGGTTTGTTTCCACAGAAACACATCTCTTGGGCTAGGGATGTGCCCTCAAAGCCGTTGGCAGAAGGTTCCCAAGGGCTCCTGGTCAAGGTCCATGTCACCTTTTGAGCACCAACTCTTAGATATACGAACACATGGTTCATCCTTAGAGAGCTTGGAGGTGTCCATTGAAGAAGAATTTGTTAAATATCGAACGACGTTTTTGATATTGGGCATAATCATGATGAGCTTGGCGTCCCTCCTGAGCAATTCATTAGTATTTTATTACGGCAGTGGAATGGCAATTGGGGTGGTCCTTGTAATATTGATTGTACTTTTTCAGGGGATGAAGCTTCTTCCAACTGGTCGGAAGAATTCTCTTGCAATTTTTGTGTACTCATCTGTGGTTGGTTTGGGATCTTTTCTCCTCAGCTACCTACCTGGGTTATTGCGTACAGTACTCATAGAGATTGGAATCAGCGAAGATATGTATAATCCTTTGGCTATATTTCTTCTGGCTTTTATTTTTCTGGCTGGAGCATGGTTGGGCTTCTGGGCAGTCCACAAACTCGTCCTTACAGAAGATGGATCAATCGATATAATGACATCTCAGTTTGTCAATTGGTCCATCAAAATTCTGGGTGCTACTTTGATTTTTCAGAGTTCTGTCGATCACCTACTGGCAACAGAAGCTATAGTATTTGGATTTGTCGTCTCTGCTATACTAAAGAAAATCTTTAGATGGAGATTCCTTCGTCGCGTGTGCAAGATTATGTCTTTCCAATTCTGGAGCTTAGTCATGGCCTTCCAGTATCTGCCAACATTTCCTTCGTCGATATCTACCGGGAAATCGCTGAAAACACCCAAAAAGAACCGCCGCAGATTGGAAATTCCTGATCCTCCGCCTTCTCCGCCTTCTCCACCTTCAGATGCATGGTTATTTCCTTCTACCTTCCACACCACACCTGAAAGGAGAAAATACTCGAAAGAAGAGTGGGACGCGTTCACCAGAGACACAACAACAAAAGCCTTGCAGGAACTTGCTTCTTCTCCTGAGTATCACAGATGGTGCTCCTCAAATGTAGAAAGAATCAGCGTCGCTCCCCGGAGCACTAGAAAAGTTGCTGATCAACCGCGCCACTGGTGGCTCCTTTGGTTGTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NEMP

Protein Analysis

483

Amino Acids

54.25

Weight (kDa)

9.81

Isoelectric Point (pI)

55.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NEMP PF10225 155 - 356 2.1e-34 NEMP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 295
AccII CGCG 4 cut(s) 146, 1044, 1286, 1420
AciI CCGC 7 cut(s) 65, 75, 1159, 1162, 1187, 1196, 1418
AclWI GGATC 4 cut(s) 32, 709, 880, 1175
AcsI RAATTY 6 cut(s) 13, 280, 482, 658, 918, 1173
AcuI CTGAAG 1 cut(s) 1194
AcyI GRCGYC 1 cut(s) 539
AfaI GTAC 4 cut(s) 618, 680, 739, 744
AfiI CCNNNNNNNGG 2 cut(s) 393, 1168
AflIII ACRYGT 1 cut(s) 1284
AgsI TTSAA 2 cut(s) 285, 476
AjnI CCWGG 4 cut(s) 163, 175, 386, 724
AjuI GAANNNNNNNTTGG 2 cut(s) 1096, 1128
AluBI AGCT 6 cut(s) 459, 534, 637, 717, 977, 1077
AluI AGCT 6 cut(s) 459, 534, 637, 717, 977, 1077
Alw21I GWGCWC 3 cut(s) 417, 1358, 1397
Alw26I GTCTC 2 cut(s) 1003, 1290
AlwI GGATC 4 cut(s) 32, 709, 880, 1175
AlwNI CAGNNNCTG 1 cut(s) 1099
AoxI GGCC 1 cut(s) 1086
ApoI RAATTY 6 cut(s) 13, 280, 482, 658, 918, 1173
AscI GGCGCGCC 1 cut(s) 144
Asp700I GAANNNNTTC 2 cut(s) 201, 280
AspLEI GCGC 3 cut(s) 146, 148, 1422
AspS9I GGNCC 3 cut(s) 396, 598, 909
AsuC2I CCSGG 2 cut(s) 1129, 1390
AsuHPI GGTGA 5 cut(s) 268, 274, 396, 950, 1282
AvaII GGWCC 3 cut(s) 396, 598, 909
BaeGI GKGCMC 1 cut(s) 353
BanII GRGCYC 1 cut(s) 386
BauI CACGAG 1 cut(s) 138
Bbv12I GWGCWC 3 cut(s) 417, 1358, 1397
BbvCI CCTCAGC 1 cut(s) 713
BccI CCATC 4 cut(s) 863, 920, 1020, 1344
BceAI ACGGC 3 cut(s) 62, 345, 589
BciT130I CCWGG 4 cut(s) 165, 177, 388, 726
BclI TGATCA 1 cut(s) 1411
BcnI CCSGG 2 cut(s) 1129, 1390
BcoDI GTCTC 2 cut(s) 1003, 1290
BfaI CTAG 2 cut(s) 341, 1398
BfmI CTRYAG 1 cut(s) 978
BisI GCNGC 1 cut(s) 1162
BlsI GCNGC 1 cut(s) 1163
BmcAI AGTACT 1 cut(s) 744
Bme1390I CCNGG 6 cut(s) 165, 177, 388, 726, 1129, 1390
Bme18I GGWCC 3 cut(s) 396, 598, 909
BmgT120I GGNCC 3 cut(s) 396, 598, 909
BmiI GGNNCC 4 cut(s) 169, 375, 385, 1433
BmrFI CCNGG 6 cut(s) 165, 177, 388, 726, 1129, 1390
BmsI GCATC 1 cut(s) 1204
BpmI CTGGAG 2 cut(s) 840, 1093
Bpu10I CCTNAGC 2 cut(s) 548, 713
BpuMI CCSGG 2 cut(s) 1129, 1390
Bsa29I ATCGAT 1 cut(s) 879
BsaBI GATNNNNATC 1 cut(s) 758
BsaHI GRCGYC 1 cut(s) 539
BsaJI CCNNGG 4 cut(s) 211, 378, 725, 1388
BsaXI ACNNNNNCTCC 2 cut(s) 145, 175
Bsc4I CCNNNNNNNGG 2 cut(s) 393, 1168
Bse1I ACTGG 5 cut(s) 149, 652, 969, 1093, 1430
Bse8I GATNNNNATC 1 cut(s) 758
BseBI CCWGG 4 cut(s) 165, 177, 388, 726
BseCI ATCGAT 1 cut(s) 879
BseDI CCNNGG 4 cut(s) 211, 378, 725, 1388
BseGI GGATG 3 cut(s) 351, 446, 636
BseJI GATNNNNATC 1 cut(s) 758
BseLI CCNNNNNNNGG 2 cut(s) 393, 1168
BseMII CTCAG 4 cut(s) 539, 727, 908, 1329
BseNI ACTGG 5 cut(s) 149, 652, 969, 1093, 1430
BsePI GCGCGC 1 cut(s) 144
BseRI GAGGAG 2 cut(s) 701, 1348
BseSI GKGCMC 1 cut(s) 353
Bsh1236I CGCG 4 cut(s) 146, 1044, 1286, 1420
BshFI GGCC 1 cut(s) 1088
BshVI ATCGAT 1 cut(s) 879
BsiHKAI GWGCWC 3 cut(s) 417, 1358, 1397
BsiSI CCGG 2 cut(s) 1128, 1390
BslFI GGGAC 2 cut(s) 526, 1295
BslI CCNNNNNNNGG 2 cut(s) 393, 1168
BsmAI GTCTC 2 cut(s) 1003, 1290
BsmBI CGTCTC 1 cut(s) 1003
BsmFI GGGAC 2 cut(s) 526, 1295
BsmI GAATGC 1 cut(s) 97
BsnI GGCC 1 cut(s) 1088
Bsp1286I GDGCHC 5 cut(s) 353, 386, 417, 1358, 1397
Bsp143I GATC 6 cut(s) 24, 701, 872, 955, 1180, 1411
BspACI CCGC 7 cut(s) 65, 75, 1159, 1162, 1187, 1196, 1418
BspANI GGCC 1 cut(s) 1088
BspCNI CTCAG 4 cut(s) 540, 726, 907, 1330
BspDI ATCGAT 1 cut(s) 879
BspFNI CGCG 4 cut(s) 146, 1044, 1286, 1420
BspHI TCATGA 1 cut(s) 525
BspLI GGNNCC 4 cut(s) 169, 375, 385, 1433
BspPI GGATC 4 cut(s) 32, 709, 880, 1175
BsrI ACTGG 5 cut(s) 149, 652, 969, 1093, 1430
BssECI CCNNGG 4 cut(s) 211, 378, 725, 1388
BssHII GCGCGC 1 cut(s) 144
BssMI GATC 6 cut(s) 24, 701, 872, 955, 1180, 1411
BssNAI GTATAC 1 cut(s) 296
BssNI GRCGYC 1 cut(s) 539
BssSI CACGAG 1 cut(s) 138
BssT1I CCWWGG 1 cut(s) 378
Bst1107I GTATAC 1 cut(s) 296
Bst2BI CACGAG 1 cut(s) 138
Bst2UI CCWGG 4 cut(s) 165, 177, 388, 726
Bst4CI ACNGT 2 cut(s) 256, 742
Bst6I CTCTTC 1 cut(s) 1269
BstACI GRCGYC 1 cut(s) 539
BstC8I GCNNGC 2 cut(s) 34, 146
BstDEI CTNAG 8 cut(s) 103, 424, 451, 548, 713, 894, 1078, 1338
BstDSI CCRYGG 1 cut(s) 211
BstENI CCTNNNNNAGG 1 cut(s) 391
BstF5I GGATG 3 cut(s) 351, 446, 636
BstFNI CGCG 4 cut(s) 146, 1044, 1286, 1420
BstHHI GCGC 3 cut(s) 146, 148, 1422
BstKTI GATC 6 cut(s) 27, 704, 875, 958, 1183, 1414
BstMAI GTCTC 2 cut(s) 1003, 1290
BstMBI GATC 6 cut(s) 24, 701, 872, 955, 1180, 1411
BstMWI GCNNNNNNNGC 3 cut(s) 357, 974, 1195
BstNI CCWGG 4 cut(s) 165, 177, 388, 726
BstSCI CCNGG 6 cut(s) 163, 175, 386, 724, 1127, 1388
BstSFI CTRYAG 1 cut(s) 978
BstSLI GKGCMC 1 cut(s) 353
BstUI CGCG 4 cut(s) 146, 1044, 1286, 1420
BstX2I RGATCY 2 cut(s) 24, 701
BstYI RGATCY 2 cut(s) 24, 701
BstZ17I GTATAC 1 cut(s) 296
Bsu15I ATCGAT 1 cut(s) 879
BsuRI GGCC 1 cut(s) 1088
BsuTUI ATCGAT 1 cut(s) 879
BtgI CCRYGG 1 cut(s) 211
BtsCI GGATG 3 cut(s) 351, 446, 636
BtsI GCAGTG 1 cut(s) 583
BtsIMutI CAGTG 3 cut(s) 192, 583, 1423
Cac8I GCNNGC 2 cut(s) 34, 146
CaiI CAGNNNCTG 1 cut(s) 1099
CciI TCATGA 1 cut(s) 525
CfoI GCGC 3 cut(s) 146, 148, 1422
Cfr13I GGNCC 3 cut(s) 396, 598, 909
ClaI ATCGAT 1 cut(s) 879
CseI GACGC 3 cut(s) 528, 1292, 1369
Csp6I GTAC 4 cut(s) 617, 679, 738, 743
CspCI CAANNNNNGTGG 2 cut(s) 576, 611
CviAII CATG 6 cut(s) 400, 440, 526, 825, 1084, 1218
CviQI GTAC 4 cut(s) 617, 679, 738, 743
DdeI CTNAG 8 cut(s) 103, 424, 451, 548, 713, 894, 1078, 1338
DpnI GATC 6 cut(s) 26, 703, 874, 957, 1182, 1413
DpnII GATC 6 cut(s) 24, 701, 872, 955, 1180, 1411
Eam1104I CTCTTC 1 cut(s) 1269
EarI CTCTTC 1 cut(s) 1269
EciI GGCGGA 3 cut(s) 64, 1176, 1185
Eco130I CCWWGG 1 cut(s) 378
Eco24I GRGCYC 1 cut(s) 386
Eco32I GATATC 1 cut(s) 1122
Eco47I GGWCC 3 cut(s) 396, 598, 909
Eco57I CTGAAG 1 cut(s) 1194
EcoNI CCTNNNNNAGG 1 cut(s) 391
EcoRI GAATTC 1 cut(s) 658
EcoRII CCWGG 4 cut(s) 163, 175, 386, 724
EcoRV GATATC 1 cut(s) 1122
EcoT14I CCWWGG 1 cut(s) 378
EcoT22I ATGCAT 1 cut(s) 1219
EcoT38I GRGCYC 1 cut(s) 386
ErhI CCWWGG 1 cut(s) 378
Esp3I CGTCTC 1 cut(s) 1003
FaeI CATG 6 cut(s) 403, 443, 529, 828, 1087, 1221
FalI AAGNNNNNCTT 2 cut(s) 1004, 1036
FaqI GGGAC 2 cut(s) 526, 1295
FatI CATG 6 cut(s) 399, 439, 525, 824, 1083, 1217
FauI CCCGC 1 cut(s) 72
FbaI TGATCA 1 cut(s) 1411
FblI GTMKAC 1 cut(s) 295
Fnu4HI GCNGC 1 cut(s) 1162
FokI GGATG 3 cut(s) 358, 433, 643
FriOI GRGCYC 1 cut(s) 386
Fsp4HI GCNGC 1 cut(s) 1162
FspBI CTAG 2 cut(s) 341, 1398
GlaI GCGC 3 cut(s) 145, 147, 1421
GluI GCNGC 1 cut(s) 1162
GsuI CTGGAG 2 cut(s) 840, 1093
HaeIII GGCC 1 cut(s) 1088
HapII CCGG 2 cut(s) 1128, 1390
HgaI GACGC 3 cut(s) 528, 1292, 1369
HhaI GCGC 3 cut(s) 146, 148, 1422
Hin1I GRCGYC 1 cut(s) 539
Hin1II CATG 6 cut(s) 403, 443, 529, 828, 1087, 1221
Hin6I GCGC 3 cut(s) 144, 146, 1420
HinP1I GCGC 3 cut(s) 144, 146, 1420
HindIII AAGCTT 1 cut(s) 635
HinfI GANTC 4 cut(s) 116, 759, 1032, 1374
HpaII CCGG 2 cut(s) 1128, 1390
HphI GGTGA 5 cut(s) 268, 274, 396, 950, 1282
Hpy166II GTNNAC 4 cut(s) 296, 679, 847, 1290
Hpy188I TCNGA 6 cut(s) 24, 115, 121, 654, 945, 1213
Hpy188III TCNNGA 6 cut(s) 273, 526, 547, 1072, 1178, 1337
Hpy8I GTNNAC 4 cut(s) 296, 679, 847, 1290
Hpy99I CGWCG 5 cut(s) 83, 505, 1044, 1120, 1385
HpyCH4III ACNGT 2 cut(s) 256, 742
HpyCH4IV ACGT 1 cut(s) 503
HpyCH4V TGCA 5 cut(s) 11, 668, 1050, 1217, 1318
HpyF10VI GCNNNNNNNGC 3 cut(s) 357, 974, 1195
HpyF3I CTNAG 8 cut(s) 103, 424, 451, 548, 713, 894, 1078, 1338
HpySE526I ACGT 1 cut(s) 503
Hsp92I GRCGYC 1 cut(s) 539
Hsp92II CATG 6 cut(s) 403, 443, 529, 828, 1087, 1221
HspAI GCGC 3 cut(s) 144, 146, 1420
Ksp22I TGATCA 1 cut(s) 1411
Kzo9I GATC 6 cut(s) 24, 701, 872, 955, 1180, 1411
LmnI GCTCC 9 cut(s) 66, 173, 389, 821, 1074, 1361, 1390, 1392, 1437
LweI GCATC 1 cut(s) 1204
MaeI CTAG 2 cut(s) 341, 1398
MaeII ACGT 1 cut(s) 503
MaeIII GTNAC 3 cut(s) 126, 151, 402
MalI GATC 6 cut(s) 26, 703, 874, 957, 1182, 1413
MboI GATC 6 cut(s) 24, 701, 872, 955, 1180, 1411
MfeI CAATTG 2 cut(s) 588, 904
MflI RGATCY 2 cut(s) 24, 701
MhlI GDGCHC 5 cut(s) 353, 386, 417, 1358, 1397
MluI ACGCGT 1 cut(s) 1284
MlyI GAGTC 1 cut(s) 125
MmeI TCCRAC 3 cut(s) 99, 632, 668
MnlI CCTC 9 cut(s) 82, 97, 301, 363, 457, 554, 722, 1194, 1369
Mph1103I ATGCAT 1 cut(s) 1219
MroXI GAANNNNTTC 2 cut(s) 201, 280
MseI TTAA 2 cut(s) 228, 489
MslI CAYNNNNRTG 3 cut(s) 185, 210, 524
MspI CCGG 2 cut(s) 1128, 1390
MspR9I CCNGG 6 cut(s) 165, 177, 388, 726, 1129, 1390
MunI CAATTG 2 cut(s) 588, 904
Mva1269I GAATGC 1 cut(s) 97
MvaI CCWGG 4 cut(s) 165, 177, 388, 726
MvnI CGCG 4 cut(s) 146, 1044, 1286, 1420
MwoI GCNNNNNNNGC 3 cut(s) 357, 974, 1195
NciI CCSGG 2 cut(s) 1129, 1390
NdeII GATC 6 cut(s) 24, 701, 872, 955, 1180, 1411
NlaIII CATG 6 cut(s) 403, 443, 529, 828, 1087, 1221
NlaIV GGNNCC 4 cut(s) 169, 375, 385, 1433
NmuCI GTSAC 1 cut(s) 402
NsiI ATGCAT 1 cut(s) 1219
PagI TCATGA 1 cut(s) 525
PalAI GGCGCGCC 1 cut(s) 144
PauI GCGCGC 1 cut(s) 144
PctI GAATGC 1 cut(s) 97
PdmI GAANNNNTTC 2 cut(s) 201, 280
PfeI GAWTC 3 cut(s) 759, 1032, 1374
PkrI GCNGC 1 cut(s) 1163
PleI GAGTC 1 cut(s) 124
PpsI GAGTC 1 cut(s) 124
Psp6I CCWGG 4 cut(s) 163, 175, 386, 724
PspGI CCWGG 4 cut(s) 163, 175, 386, 724
PspN4I GGNNCC 4 cut(s) 169, 375, 385, 1433
PspPI GGNCC 3 cut(s) 396, 598, 909
PstNI CAGNNNCTG 1 cut(s) 1099
PsuI RGATCY 2 cut(s) 24, 701
PteI GCGCGC 1 cut(s) 144
RsaI GTAC 4 cut(s) 618, 680, 739, 744
RsaNI GTAC 4 cut(s) 617, 679, 738, 743
RseI CAYNNNNRTG 3 cut(s) 185, 210, 524
SaqAI TTAA 2 cut(s) 228, 489
SatI GCNGC 1 cut(s) 1162
Sau3AI GATC 6 cut(s) 24, 701, 872, 955, 1180, 1411
Sau96I GGNCC 3 cut(s) 396, 598, 909
ScaI AGTACT 1 cut(s) 744
SchI GAGTC 1 cut(s) 125
ScrFI CCNGG 6 cut(s) 165, 177, 388, 726, 1129, 1390
SduI GDGCHC 5 cut(s) 353, 386, 417, 1358, 1397
SfaNI GCATC 1 cut(s) 1204
SfcI CTRYAG 1 cut(s) 978
SgsI GGCGCGCC 1 cut(s) 144
SinI GGWCC 3 cut(s) 396, 598, 909
SmiMI CAYNNNNRTG 3 cut(s) 185, 210, 524
SsiI CCGC 7 cut(s) 65, 75, 1159, 1162, 1187, 1196, 1418
SspI AATATT 2 cut(s) 235, 609
SspMI CTAG 2 cut(s) 341, 1398
StyD4I CCNGG 6 cut(s) 163, 175, 386, 724, 1127, 1388
StyI CCWWGG 1 cut(s) 378
TaaI ACNGT 2 cut(s) 256, 742
TaiI ACGT 1 cut(s) 506
TaqI TCGA 6 cut(s) 81, 496, 879, 954, 1118, 1268
TatI WGTACW 3 cut(s) 616, 678, 742
TauI GCSGC 1 cut(s) 1164
TfiI GAWTC 3 cut(s) 759, 1032, 1374
Tru1I TTAA 2 cut(s) 228, 489
Tru9I TTAA 2 cut(s) 228, 489
TscAI CASTG 3 cut(s) 192, 583, 1430
TseFI GTSAC 1 cut(s) 402
Tsp45I GTSAC 1 cut(s) 402
TspDTI ATGAA 4 cut(s) 194, 435, 546, 647
TspGWI ACGGA 1 cut(s) 200
TspRI CASTG 3 cut(s) 192, 583, 1430
VpaK11BI GGWCC 3 cut(s) 396, 598, 909
XagI CCTNNNNNAGG 1 cut(s) 391
XapI RAATTY 6 cut(s) 13, 280, 482, 658, 918, 1173
XcmI CCANNNNNNNNNTGG 1 cut(s) 919
XmiI GTMKAC 1 cut(s) 295
XmnI GAANNNNTTC 2 cut(s) 201, 280
XspI CTAG 2 cut(s) 341, 1398
ZrmI AGTACT 1 cut(s) 744
Zsp2I ATGCAT 1 cut(s) 1219
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.