Rw5G036750

NEMP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0001753
Physical Location & Seq
Forward (+)
64508095 .. 64515158
7064 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G036750.1

Sequence Viewer

Length: 1503 bp
ATGGCTTCTTCTAAGCAGGTTCAGGTCACAACTTGTATTCTATGGCTTCTGTTCCTCACACTTCCATGTTCTTCTTCCACTGAATCACTCTATCAGCATTCTTTGGTTGTTGGTGAAAATGCTACTTTAGGATTGTCTCATGGTATGCCAATCGGAAAATCTCCCGGTTCTAAGCCTGGCGCTTCGGTGTTGTGTGAACGAGTTCTTATCTATGGATTGTCAAGGCTTAAACATCCAGATAAGTTTGCCAATACAGTGAAGGTGAAGATATCAAAAACATCAAGATTTCAGACTTTTGAGGTTTGTTTCCATAGAAATACGTCCCGTGGGATAGGAATGTGCCCTGACAGCCAGTGGGAGAAAGTTTCCAAGGGGTCCTGGTCTCGATCCATGTCACCTTTTGAGCACAAACTCTTGGATATACGAACAGCTGGTTCATCCTTACAGAATTTTGATGTGTTCATTAAAGAAGAATTTTTCTTATCTCGCTTAATATATTTTGTATCTGGCATAGTCCTGATGACCTTGGCGCCTTACCTGAGTAACTCTTTAGTGTTTTATTACAGCAGCGGAATGGCAATAGGGGTGGTTCTTGTAATATTGATTTTCCTTCTTCAGGTAATGAAGCTTCTCCCAACTGGTCGGAAGAATTCACTCGCAATTTTCGTCTTGTCATCTGTGGTTGGTTTGGGATCTTTTCTCCTCCGCTATATCCCAGGACTACTATGTTCAGTACTCACAGAGCTTGGAATTAGTGAGGATATGTATAATGTTTTGACCATATTTATTCTGGCTTTTGTTTTTCTGGCTGGAGCATCGTTGGGATTATGGACAGTTCGTAAAATGGCCTTGAACGAAGATGGATCAATTGATTCTTGCATATCTCTGTTTGTTTTGTGGACCATTCGCTTCGTAGCTGTTATTTTTGTTTCACAGATCATTTTCATAGCGAGGACATTCACTATAGTATGTGGATGCATAATCTCTTCAGCAAGGGAAATCTTTAGATGGATCCGTGGTCGTCACATGTACAGGAAATTGTTGAGATCTGTTAGAACCACAGATTCTTTGCATATCTCTTCCTCAAATCAAGATTCAAGTGCAATTGCACAAGTCAATGAAAATCAATTTCTTCAAGATCACCCAAAAAAGAAGAGCAGAAGATTAGAGATTCCTGATTCTTCACCTTCTGAATGTGCTCCTAATGAAGTCGTTAACGAGTTTCAGAGCAATGAGGACTATAAGCTACCGAGGCCTGGTTCCAGAAACTCTACCTCGGCTTCACGCATTTCACCTGGCCTAGGTTCTAAAACAATGACAAAACATGAGTGGGACAGTCTCACTAGACACACAACAGAGAAGGCCTTGGAAGAACTTGCTTCTTCTCCTGATTTTGGCAAATGGGTTGTTGCAAATGCAGATAGAATCAATGTAACCACCCTTAGTTTGCGAGGTCGTCACCCGACAACAAATAGGACAGAAACTGTGCGTTTAAATCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NEMP

Protein Analysis

500

Amino Acids

55.78

Weight (kDa)

9.51

Isoelectric Point (pI)

45.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NEMP PF10225 155 - 390 6.6e-33 NEMP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 7
AccB1I GGYRCC 1 cut(s) 529
AciI CCGC 2 cut(s) 570, 706
AclWI GGATC 5 cut(s) 381, 700, 871, 1006, 1019
AcsI RAATTY 3 cut(s) 448, 473, 649
AcuI CTGAAG 2 cut(s) 599, 972
AcyI GRCGYC 1 cut(s) 530
AfaI GTAC 2 cut(s) 735, 1031
AfiI CCNNNNNNNGG 4 cut(s) 616, 1256, 1301, 1394
AflIII ACRYGT 1 cut(s) 1026
AgsI TTSAA 3 cut(s) 853, 1098, 1136
AjnI CCWGG 5 cut(s) 175, 377, 715, 1255, 1294
AluBI AGCT 5 cut(s) 431, 628, 745, 917, 1246
AluI AGCT 5 cut(s) 431, 628, 745, 917, 1246
Alw21I GWGCWC 2 cut(s) 408, 1201
Alw26I GTCTC 3 cut(s) 141, 387, 1343
AlwI GGATC 5 cut(s) 381, 700, 871, 1006, 1019
AlwNI CAGNNNCTG 1 cut(s) 1484
AoxI GGCC 4 cut(s) 846, 1253, 1297, 1362
ApeKI GCWGC 1 cut(s) 567
ApoI RAATTY 3 cut(s) 448, 473, 649
Asp700I GAANNNNTTC 1 cut(s) 201
AspA2I CCTAGG 1 cut(s) 1300
AspLEI GCGC 2 cut(s) 182, 532
AspS9I GGNCC 2 cut(s) 375, 900
AsuC2I CCSGG 1 cut(s) 165
AsuHPI GGTGA 7 cut(s) 125, 274, 387, 1133, 1176, 1284, 1451
AvaII GGWCC 2 cut(s) 375, 900
AvrII CCTAGG 1 cut(s) 1300
BaeGI GKGCMC 1 cut(s) 344
BamHI GGATCC 1 cut(s) 1011
BanI GGYRCC 1 cut(s) 529
BarI GAAGNNNNNNTAC 2 cut(s) 612, 644
Bbv12I GWGCWC 2 cut(s) 408, 1201
BbvI GCAGC 1 cut(s) 579
BccI CCATC 2 cut(s) 854, 1002
BciT130I CCWGG 5 cut(s) 177, 379, 717, 1257, 1296
BcnI CCSGG 1 cut(s) 165
BcoDI GTCTC 3 cut(s) 141, 387, 1343
BfaI CTAG 2 cut(s) 1301, 1344
BfmI CTRYAG 1 cut(s) 963
BfoI RGCGCY 2 cut(s) 183, 533
BfuAI ACCTGC 1 cut(s) 7
BglII AGATCT 1 cut(s) 1046
BisI GCNGC 1 cut(s) 568
BlnI CCTAGG 1 cut(s) 1300
BlsI GCNGC 1 cut(s) 569
BmcAI AGTACT 1 cut(s) 735
Bme1390I CCNGG 6 cut(s) 165, 177, 379, 717, 1257, 1296
Bme18I GGWCC 2 cut(s) 375, 900
BmgT120I GGNCC 2 cut(s) 375, 900
BmiI GGNNCC 4 cut(s) 376, 531, 1013, 1261
BmrFI CCNGG 6 cut(s) 165, 177, 379, 717, 1257, 1296
BmsI GCATC 2 cut(s) 824, 965
BpmI CTGGAG 1 cut(s) 831
BpuMI CCSGG 1 cut(s) 165
BsaHI GRCGYC 1 cut(s) 530
BsaI GGTCTC 1 cut(s) 387
BsaJI CCNNGG 9 cut(s) 325, 369, 525, 715, 1015, 1250, 1275, 1300, 1365
Bsc4I CCNNNNNNNGG 4 cut(s) 616, 1256, 1301, 1394
Bse1I ACTGG 2 cut(s) 352, 643
Bse3DI GCAATG 1 cut(s) 1237
BseBI CCWGG 5 cut(s) 177, 379, 717, 1257, 1296
BseDI CCNNGG 9 cut(s) 325, 369, 525, 715, 1015, 1250, 1275, 1300, 1365
BseGI GGATG 3 cut(s) 232, 437, 980
BseLI CCNNNNNNNGG 4 cut(s) 616, 1256, 1301, 1394
BseMI GCAATG 1 cut(s) 1237
BseMII CTCAG 1 cut(s) 530
BseNI ACTGG 2 cut(s) 352, 643
BseRI GAGGAG 1 cut(s) 692
BseSI GKGCMC 1 cut(s) 344
BseXI GCAGC 1 cut(s) 579
BshFI GGCC 4 cut(s) 848, 1255, 1299, 1364
BshNI GGYRCC 1 cut(s) 529
BsiHKAI GWGCWC 2 cut(s) 408, 1201
BsiSI CCGG 1 cut(s) 165
BslFI GGGAC 2 cut(s) 307, 1346
BslI CCNNNNNNNGG 4 cut(s) 616, 1256, 1301, 1394
BsmAI GTCTC 3 cut(s) 141, 387, 1343
BsmFI GGGAC 2 cut(s) 307, 1346
BsmI GAATGC 1 cut(s) 97
BsnI GGCC 4 cut(s) 848, 1255, 1299, 1364
Bso31I GGTCTC 1 cut(s) 387
Bsp1286I GDGCHC 3 cut(s) 344, 408, 1201
Bsp1407I TGTACA 1 cut(s) 1029
Bsp143I GATC 7 cut(s) 386, 692, 863, 936, 1011, 1046, 1138
BspACI CCGC 2 cut(s) 570, 706
BspANI GGCC 4 cut(s) 848, 1255, 1299, 1364
BspCNI CTCAG 1 cut(s) 531
BspLI GGNNCC 4 cut(s) 376, 531, 1013, 1261
BspMI ACCTGC 1 cut(s) 7
BspPI GGATC 5 cut(s) 381, 700, 871, 1006, 1019
BspQI GCTCTTC 1 cut(s) 1148
BspT107I GGYRCC 1 cut(s) 529
BspTNI GGTCTC 1 cut(s) 387
BsrDI GCAATG 1 cut(s) 1237
BsrGI TGTACA 1 cut(s) 1029
BsrI ACTGG 2 cut(s) 352, 643
BssECI CCNNGG 9 cut(s) 325, 369, 525, 715, 1015, 1250, 1275, 1300, 1365
BssMI GATC 7 cut(s) 386, 692, 863, 936, 1011, 1046, 1138
BssNI GRCGYC 1 cut(s) 530
BssT1I CCWWGG 4 cut(s) 369, 525, 1300, 1365
Bst2UI CCWGG 5 cut(s) 177, 379, 717, 1257, 1296
Bst4CI ACNGT 4 cut(s) 256, 835, 1337, 1486
Bst6I CTCTTC 3 cut(s) 991, 1084, 1148
BstACI GRCGYC 1 cut(s) 530
BstAUI TGTACA 1 cut(s) 1029
BstDEI CTNAG 4 cut(s) 12, 171, 539, 1442
BstDSI CCRYGG 2 cut(s) 325, 1015
BstENI CCTNNNNNAGG 2 cut(s) 614, 1299
BstF5I GGATG 3 cut(s) 232, 437, 980
BstH2I RGCGCY 2 cut(s) 183, 533
BstHHI GCGC 2 cut(s) 182, 532
BstKTI GATC 7 cut(s) 389, 695, 866, 939, 1014, 1049, 1141
BstMAI GTCTC 3 cut(s) 141, 387, 1343
BstMBI GATC 7 cut(s) 386, 692, 863, 936, 1011, 1046, 1138
BstMWI GCNNNNNNNGC 2 cut(s) 348, 1252
BstNI CCWGG 5 cut(s) 177, 379, 717, 1257, 1296
BstNSI RCATGY 1 cut(s) 1030
BstSCI CCNGG 6 cut(s) 163, 175, 377, 715, 1255, 1294
BstSFI CTRYAG 1 cut(s) 963
BstSLI GKGCMC 1 cut(s) 344
BstV1I GCAGC 1 cut(s) 579
BstX2I RGATCY 3 cut(s) 692, 1011, 1046
BstYI RGATCY 3 cut(s) 692, 1011, 1046
BsuRI GGCC 4 cut(s) 848, 1255, 1299, 1364
BtgI CCRYGG 2 cut(s) 325, 1015
BtsCI GGATG 3 cut(s) 232, 437, 980
BtsIMutI CAGTG 3 cut(s) 78, 261, 359
BveI ACCTGC 1 cut(s) 7
CaiI CAGNNNCTG 1 cut(s) 1484
CfoI GCGC 2 cut(s) 182, 532
Cfr13I GGNCC 2 cut(s) 375, 900
Csp6I GTAC 2 cut(s) 734, 1030
CspCI CAANNNNNGTGG 2 cut(s) 567, 602
CviAII CATG 5 cut(s) 66, 140, 391, 1027, 1325
CviQI GTAC 2 cut(s) 734, 1030
DdeI CTNAG 4 cut(s) 12, 171, 539, 1442
DinI GGCGCC 1 cut(s) 531
DpnI GATC 7 cut(s) 388, 694, 865, 938, 1013, 1048, 1140
DpnII GATC 7 cut(s) 386, 692, 863, 936, 1011, 1046, 1138
DraI TTTAAA 1 cut(s) 1494
Eam1104I CTCTTC 3 cut(s) 991, 1084, 1148
EarI CTCTTC 3 cut(s) 991, 1084, 1148
Eco130I CCWWGG 4 cut(s) 369, 525, 1300, 1365
Eco147I AGGCCT 2 cut(s) 1255, 1364
Eco31I GGTCTC 1 cut(s) 387
Eco32I GATATC 1 cut(s) 270
Eco47I GGWCC 2 cut(s) 375, 900
Eco57I CTGAAG 2 cut(s) 599, 972
EcoNI CCTNNNNNAGG 2 cut(s) 614, 1299
EcoO109I RGGNCCY 1 cut(s) 375
EcoRI GAATTC 1 cut(s) 649
EcoRII CCWGG 5 cut(s) 175, 377, 715, 1255, 1294
EcoRV GATATC 1 cut(s) 270
EcoT14I CCWWGG 4 cut(s) 369, 525, 1300, 1365
EcoT22I ATGCAT 1 cut(s) 980
EgeI GGCGCC 1 cut(s) 531
EheI GGCGCC 1 cut(s) 531
ErhI CCWWGG 4 cut(s) 369, 525, 1300, 1365
FaeI CATG 5 cut(s) 69, 143, 394, 1030, 1328
FaqI GGGAC 2 cut(s) 307, 1346
FatI CATG 5 cut(s) 65, 139, 390, 1026, 1324
Fnu4HI GCNGC 1 cut(s) 568
FokI GGATG 3 cut(s) 219, 424, 987
Fsp4HI GCNGC 1 cut(s) 568
FspBI CTAG 2 cut(s) 1301, 1344
GlaI GCGC 2 cut(s) 181, 531
GluI GCNGC 1 cut(s) 568
GsuI CTGGAG 1 cut(s) 831
HaeII RGCGCY 2 cut(s) 183, 533
HaeIII GGCC 4 cut(s) 848, 1255, 1299, 1364
HapII CCGG 1 cut(s) 165
HhaI GCGC 2 cut(s) 182, 532
Hin1I GRCGYC 1 cut(s) 530
Hin1II CATG 5 cut(s) 69, 143, 394, 1030, 1328
Hin6I GCGC 2 cut(s) 180, 530
HinP1I GCGC 2 cut(s) 180, 530
HincII GTYRAC 1 cut(s) 1216
HindII GTYRAC 1 cut(s) 1216
HindIII AAGCTT 1 cut(s) 626
HinfI GANTC 7 cut(s) 83, 872, 1064, 1094, 1171, 1178, 1425
HpaI GTTAAC 1 cut(s) 1216
HpaII CCGG 1 cut(s) 165
HphI GGTGA 7 cut(s) 125, 274, 387, 1133, 1176, 1284, 1451
Hpy166II GTNNAC 3 cut(s) 197, 900, 1216
Hpy188I TCNGA 6 cut(s) 155, 291, 645, 1192, 1227, 1502
Hpy188III TCNNGA 9 cut(s) 236, 282, 384, 517, 1091, 1136, 1175, 1263, 1388
Hpy8I GTNNAC 3 cut(s) 197, 900, 1216
HpyAV CCTTC 4 cut(s) 253, 620, 1197, 1354
HpyCH4III ACNGT 4 cut(s) 256, 835, 1337, 1486
HpyCH4IV ACGT 1 cut(s) 320
HpyCH4V TGCA 7 cut(s) 879, 978, 1072, 1103, 1109, 1412, 1418
HpyF10VI GCNNNNNNNGC 2 cut(s) 348, 1252
HpyF3I CTNAG 4 cut(s) 12, 171, 539, 1442
HpySE526I ACGT 1 cut(s) 320
Hsp92I GRCGYC 1 cut(s) 530
Hsp92II CATG 5 cut(s) 69, 143, 394, 1030, 1328
HspAI GCGC 2 cut(s) 180, 530
KasI GGCGCC 1 cut(s) 529
KspAI GTTAAC 1 cut(s) 1216
Kzo9I GATC 7 cut(s) 386, 692, 863, 936, 1011, 1046, 1138
LguI GCTCTTC 1 cut(s) 1148
LmnI GCTCC 2 cut(s) 812, 1204
Lsp1109I GCAGC 1 cut(s) 579
LweI GCATC 2 cut(s) 824, 965
MaeI CTAG 2 cut(s) 1301, 1344
MaeII ACGT 1 cut(s) 320
MaeIII GTNAC 6 cut(s) 25, 393, 542, 1022, 1432, 1457
MalI GATC 7 cut(s) 388, 694, 865, 938, 1013, 1048, 1140
MboI GATC 7 cut(s) 386, 692, 863, 936, 1011, 1046, 1138
MfeI CAATTG 2 cut(s) 867, 1104
MflI RGATCY 3 cut(s) 692, 1011, 1046
MhlI GDGCHC 3 cut(s) 344, 408, 1201
MluCI AATT 9 cut(s) 448, 473, 649, 660, 750, 867, 1037, 1104, 1127
Mly113I GGCGCC 1 cut(s) 530
MmeI TCCRAC 1 cut(s) 623
Mph1103I ATGCAT 1 cut(s) 980
MroXI GAANNNNTTC 1 cut(s) 201
MseI TTAA 5 cut(s) 228, 465, 491, 1215, 1493
MslI CAYNNNNRTG 1 cut(s) 64
MspA1I CMGCKG 2 cut(s) 431, 570
MspI CCGG 1 cut(s) 165
MspR9I CCNGG 6 cut(s) 165, 177, 379, 717, 1257, 1296
MunI CAATTG 2 cut(s) 867, 1104
Mva1269I GAATGC 1 cut(s) 97
MvaI CCWGG 5 cut(s) 177, 379, 717, 1257, 1296
MwoI GCNNNNNNNGC 2 cut(s) 348, 1252
NarI GGCGCC 1 cut(s) 530
NciI CCSGG 1 cut(s) 165
NdeII GATC 7 cut(s) 386, 692, 863, 936, 1011, 1046, 1138
NlaIII CATG 5 cut(s) 69, 143, 394, 1030, 1328
NlaIV GGNNCC 4 cut(s) 376, 531, 1013, 1261
NmeAIII GCCGAG 1 cut(s) 1256
NmuCI GTSAC 4 cut(s) 25, 393, 1022, 1457
NsiI ATGCAT 1 cut(s) 980
NspI RCATGY 1 cut(s) 1030
PceI AGGCCT 2 cut(s) 1255, 1364
PciI ACATGT 1 cut(s) 1026
PciSI GCTCTTC 1 cut(s) 1148
PcsI WCGNNNNNNNCGW 1 cut(s) 663
PctI GAATGC 1 cut(s) 97
PdmI GAANNNNTTC 1 cut(s) 201
PfeI GAWTC 7 cut(s) 83, 872, 1064, 1094, 1171, 1178, 1425
PkrI GCNGC 1 cut(s) 569
PluTI GGCGCC 1 cut(s) 533
PpuMI RGGWCCY 1 cut(s) 375
PscI ACATGT 1 cut(s) 1026
Psp5II RGGWCCY 1 cut(s) 375
Psp6I CCWGG 5 cut(s) 175, 377, 715, 1255, 1294
PspGI CCWGG 5 cut(s) 175, 377, 715, 1255, 1294
PspN4I GGNNCC 4 cut(s) 376, 531, 1013, 1261
PspPI GGNCC 2 cut(s) 375, 900
PspPPI RGGWCCY 1 cut(s) 375
PstNI CAGNNNCTG 1 cut(s) 1484
PsuI RGATCY 3 cut(s) 692, 1011, 1046
PvuII CAGCTG 1 cut(s) 431
RsaI GTAC 2 cut(s) 735, 1031
RsaNI GTAC 2 cut(s) 734, 1030
RseI CAYNNNNRTG 1 cut(s) 64
SapI GCTCTTC 1 cut(s) 1148
SaqAI TTAA 5 cut(s) 228, 465, 491, 1215, 1493
SatI GCNGC 1 cut(s) 568
Sau3AI GATC 7 cut(s) 386, 692, 863, 936, 1011, 1046, 1138
Sau96I GGNCC 2 cut(s) 375, 900
ScaI AGTACT 1 cut(s) 735
ScrFI CCNGG 6 cut(s) 165, 177, 379, 717, 1257, 1296
SduI GDGCHC 3 cut(s) 344, 408, 1201
SfaNI GCATC 2 cut(s) 824, 965
SfcI CTRYAG 1 cut(s) 963
SfoI GGCGCC 1 cut(s) 531
SinI GGWCC 2 cut(s) 375, 900
SmiMI CAYNNNNRTG 1 cut(s) 64
Sse9I AATT 9 cut(s) 448, 473, 649, 660, 750, 867, 1037, 1104, 1127
SseBI AGGCCT 2 cut(s) 1255, 1364
SsiI CCGC 2 cut(s) 570, 706
SspDI GGCGCC 1 cut(s) 529
SspI AATATT 1 cut(s) 600
SspMI CTAG 2 cut(s) 1301, 1344
StuI AGGCCT 2 cut(s) 1255, 1364
StyD4I CCNGG 6 cut(s) 163, 175, 377, 715, 1255, 1294
StyI CCWWGG 4 cut(s) 369, 525, 1300, 1365
TaaI ACNGT 4 cut(s) 256, 835, 1337, 1486
TaiI ACGT 1 cut(s) 323
TaqI TCGA 1 cut(s) 385
TasI AATT 9 cut(s) 448, 473, 649, 660, 750, 867, 1037, 1104, 1127
TatI WGTACW 2 cut(s) 733, 1029
TfiI GAWTC 7 cut(s) 83, 872, 1064, 1094, 1171, 1178, 1425
Tru1I TTAA 5 cut(s) 228, 465, 491, 1215, 1493
Tru9I TTAA 5 cut(s) 228, 465, 491, 1215, 1493
TscAI CASTG 3 cut(s) 85, 261, 359
TseFI GTSAC 4 cut(s) 25, 393, 1022, 1457
TseI GCWGC 1 cut(s) 567
Tsp45I GTSAC 4 cut(s) 25, 393, 1022, 1457
TspDTI ATGAA 6 cut(s) 426, 451, 638, 934, 1134, 1221
TspGWI ACGGA 1 cut(s) 1004
TspRI CASTG 3 cut(s) 85, 261, 359
VpaK11BI GGWCC 2 cut(s) 375, 900
XagI CCTNNNNNAGG 2 cut(s) 614, 1299
XapI RAATTY 3 cut(s) 448, 473, 649
XceI RCATGY 1 cut(s) 1030
XcmI CCANNNNNNNNNTGG 1 cut(s) 787
XmaJI CCTAGG 1 cut(s) 1300
XmnI GAANNNNTTC 1 cut(s) 201
XspI CTAG 2 cut(s) 1301, 1344
ZrmI AGTACT 1 cut(s) 735
Zsp2I ATGCAT 1 cut(s) 980
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.