Rh2CG205300

NEMP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0001753
Physical Location & Seq
Reverse (-)
19968396 .. 19972297
3902 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG205300.1

Sequence Viewer

Length: 1584 bp
ATGGCTTCTTCTAAGCCGGTTCGGGTCGCTGCGTGCATTCTATGGCTTCTGTTCCTCACGCTTCCCTCTTCTTCTTCTTCCTCTGAATCGCCGACTGAGTATTCTCTAGTTATTGGTGAGAATGCTACTCTGCAATTATCGAATGGTGTGCTGGTGCAAAATTCTCCTGGTGCTAAGCCTGGAACTTCACTGTTGTGTGGAAGAGTTCATGTATGTGGATTGTCAAGGCTTAAATATCTGGAGAAGTTTGCCGACACGGTGAAGGTGAAGATATCAACAAATTCGAGCTTTCGGATACGGCCTTTTGAGGTTTGCTTCCACAGAAATACTTCTCGTGGGATAGGAATGTGCCCTCACAGCCAGTGGGAGAAAGTTTCCAAGGGGTCCTGGTCTGGATCCATGTCACCTTATGAGCGCAAACTCTTAGATATACGGACAGTGGGTTCATCCTTAGAGAGTTTTGAGGTGTCCCTTGAAGAAGAATTTTTTATATCTCGCCTAGTATTTTTTGTGTCTGGTGTAATCTTGATGAGTTTGGCGCCTTCTCTGAGCAATTCATTGGTATTCTATTACGGCAGTGGAATGGCAATAGGGGTGGTCCTTGTACTATTGATCGTCCTTTTTCAGGGGATGAAGCTTCTCCCAACAGGTCGGAAGAGTTCGCTCGGAATTTTTTTGTTTTCATCTGTGGTTGGTTTGGGATCTTTTCTCCTCAGCTACATCCCAGGGCTATTGCGTTCAGTACTAACAGAGCTTGGAATTAGCGAAGAGATGTATAATCCTTTGGCTATATTTCTTCTGGCTTTTATTTTTCTGGCTGGAGCATGGTTGGGATTCTGGACAGTTCGTAAAATGGCCCTGAGAGAAGATGGATCAATTGATTCTAGCACATCTCTGTTTGTTTTGTGGACCATCCGGTTTGTAGCTCTTCTTCTGCTTTCACAGAGTTCTGGTGATCCGCTACTGGCAGCAGGAGCCATAACGGTCCCCTTGCTCCCTTTTATAGTGAGGAAAATTGCTATAATGTTTGGAGTTATACTCTCTTTTATGAGGAAAATAACCATAGCATTTGGAACAGTAGTTTCTTTTATAGCGAGGACAATCGCTATAATATTTGGAGGTGTAATCTCTTCAGTGCGGGAAATCTTCAGATGGATTCGTCACCGTCGCATGTTCAGGAAATTGTTGAAATCACCCAAAAAGAAGAAGAGCAGAAGATCAGAGATTCCTGATTCTCCACCTTCTGAATATGCACCTGATGAGGTCATAAACGAGTTTCAAAGCGATGACGACTATAAGTTCCCGAGGCCCGGATCCAGAAACTCTACCCTGGCTTCATGTTCTAGAAGTGCATCACCATCAGAAAGGTTATACCCTTCTTATATCCACACTACACCTGAGAGGAGAGCGGTGCCAAGACATGAGTGGGAAAGTATCACCAGACACACGACGGAGAAAGCCTTGGAAGAACTTGCTTCTTCACCTGATTTCGGCAAGTGGGTAGTTGCAAATGCTGATAGAATCAGTATCACCCCACTCAGCAGCAGAAAGACTGATGAGCAGAGCCGTAGCTGGTTCTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NEMP

Protein Analysis

527

Amino Acids

58.61

Weight (kDa)

9.75

Isoelectric Point (pI)

54.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NEMP PF10225 155 - 343 1.6e-34 NEMP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 538, 1411
AccBSI CCGCTC 1 cut(s) 1409
AciI CCGC 3 cut(s) 959, 1138, 1409
AclWI GGATC 7 cut(s) 390, 403, 709, 880, 950, 1308, 1321
AcsI RAATTY 4 cut(s) 160, 280, 482, 669
AcuI CTGAAG 2 cut(s) 1116, 1132
AcyI GRCGYC 1 cut(s) 539
AfaI GTAC 2 cut(s) 606, 744
AfiI CCNNNNNNNGG 3 cut(s) 625, 1310, 1490
AgsI TTSAA 3 cut(s) 476, 1189, 1280
AjnI CCWGG 5 cut(s) 166, 178, 386, 724, 1329
AleI CACNNNNGTG 1 cut(s) 193
AloI GAACNNNNNNTCC 2 cut(s) 427, 459
AluBI AGCT 6 cut(s) 288, 637, 717, 754, 926, 1572
AluI AGCT 6 cut(s) 288, 637, 717, 754, 926, 1572
AlwI GGATC 7 cut(s) 390, 403, 709, 880, 950, 1308, 1321
Ama87I CYCGRG 1 cut(s) 1303
AoxI GGCC 3 cut(s) 299, 855, 1307
ApeKI GCWGC 3 cut(s) 29, 968, 1542
ApoI RAATTY 4 cut(s) 160, 280, 482, 669
Asp700I GAANNNNTTC 1 cut(s) 328
AspLEI GCGC 2 cut(s) 417, 541
AspS9I GGNCC 6 cut(s) 384, 598, 856, 909, 985, 1308
AsuC2I CCSGG 1 cut(s) 1311
AvaI CYCGRG 1 cut(s) 1303
AvaII GGWCC 4 cut(s) 384, 598, 909, 985
BaeGI GKGCMC 1 cut(s) 353
BamHI GGATCC 2 cut(s) 395, 1313
BanI GGYRCC 2 cut(s) 538, 1411
BarI GAAGNNNNNNTAC 2 cut(s) 915, 947
BauI CACGAG 1 cut(s) 333
BbvCI CCTCAGC 1 cut(s) 713
BbvI GCAGC 3 cut(s) 16, 980, 1554
BccI CCATC 4 cut(s) 863, 920, 1146, 1366
BceAI ACGGC 3 cut(s) 314, 589, 1551
BcgI CGANNNNNNTGC 2 cut(s) 130, 164
BciT130I CCWGG 5 cut(s) 168, 180, 388, 726, 1331
BciVI GTATCC 1 cut(s) 288
BcnI CCSGG 1 cut(s) 1311
BfaI CTAG 5 cut(s) 107, 500, 885, 1344, 1582
BfoI RGCGCY 1 cut(s) 542
BfuI GTATCC 1 cut(s) 288
BisI GCNGC 3 cut(s) 30, 969, 1543
BlpI GCTNAGC 1 cut(s) 174
BlsI GCNGC 3 cut(s) 31, 970, 1544
BmcAI AGTACT 1 cut(s) 744
Bme1390I CCNGG 6 cut(s) 168, 180, 388, 726, 1311, 1331
Bme18I GGWCC 4 cut(s) 384, 598, 909, 985
BmeT110I CYCGRG 1 cut(s) 1303
BmgT120I GGNCC 6 cut(s) 384, 598, 856, 909, 985, 1308
BmiI GGNNCC 7 cut(s) 385, 397, 540, 976, 987, 1315, 1413
BmrFI CCNGG 6 cut(s) 168, 180, 388, 726, 1311, 1331
BmsI GCATC 1 cut(s) 1361
BplI GAGNNNNNCTC 2 cut(s) 1023, 1055
BpmI CTGGAG 2 cut(s) 260, 840
Bpu10I CCTNAGC 1 cut(s) 713
Bpu1102I GCTNAGC 1 cut(s) 174
BpuMI CCSGG 1 cut(s) 1311
BsaHI GRCGYC 1 cut(s) 539
BsaJI CCNNGG 6 cut(s) 378, 724, 725, 1304, 1329, 1461
BsaWI WCCGGW 1 cut(s) 915
Bsc4I CCNNNNNNNGG 3 cut(s) 625, 1310, 1490
Bse118I RCCGGY 1 cut(s) 16
Bse1I ACTGG 2 cut(s) 361, 969
BseBI CCWGG 5 cut(s) 168, 180, 388, 726, 1331
BseDI CCNNGG 6 cut(s) 378, 724, 725, 1304, 1329, 1461
BseGI GGATG 4 cut(s) 446, 636, 720, 912
BseLI CCNNNNNNNGG 3 cut(s) 625, 1310, 1490
BseMII CTCAG 6 cut(s) 87, 539, 727, 851, 1389, 1552
BseNI ACTGG 2 cut(s) 361, 969
BseRI GAGGAG 2 cut(s) 701, 1417
BseSI GKGCMC 1 cut(s) 353
BseXI GCAGC 3 cut(s) 16, 980, 1554
BshFI GGCC 3 cut(s) 301, 857, 1309
BshNI GGYRCC 2 cut(s) 538, 1411
BsiHKCI CYCGRG 1 cut(s) 1303
BsiSI CCGG 3 cut(s) 17, 916, 1311
BslFI GGGAC 2 cut(s) 454, 971
BslI CCNNNNNNNGG 3 cut(s) 625, 1310, 1490
BsmFI GGGAC 2 cut(s) 454, 971
BsmI GAATGC 2 cut(s) 36, 127
BsnI GGCC 3 cut(s) 301, 857, 1309
BsoBI CYCGRG 1 cut(s) 1303
Bsp1286I GDGCHC 1 cut(s) 353
Bsp143I GATC 7 cut(s) 395, 612, 701, 872, 955, 1217, 1313
Bsp1720I GCTNAGC 1 cut(s) 174
BspACI CCGC 3 cut(s) 959, 1138, 1409
BspANI GGCC 3 cut(s) 301, 857, 1309
BspCNI CTCAG 6 cut(s) 88, 540, 726, 852, 1390, 1551
BspLI GGNNCC 7 cut(s) 385, 397, 540, 976, 987, 1315, 1413
BspPI GGATC 7 cut(s) 390, 403, 709, 880, 950, 1308, 1321
BspQI GCTCTTC 2 cut(s) 933, 1202
BspT107I GGYRCC 2 cut(s) 538, 1411
BsrBI CCGCTC 1 cut(s) 1409
BsrFI RCCGGY 1 cut(s) 16
BsrI ACTGG 2 cut(s) 361, 969
BssAI RCCGGY 1 cut(s) 16
BssECI CCNNGG 6 cut(s) 378, 724, 725, 1304, 1329, 1461
BssMI GATC 7 cut(s) 395, 612, 701, 872, 955, 1217, 1313
BssNI GRCGYC 1 cut(s) 539
BssSI CACGAG 1 cut(s) 333
BssT1I CCWWGG 2 cut(s) 378, 1461
Bst2BI CACGAG 1 cut(s) 333
Bst2UI CCWGG 5 cut(s) 168, 180, 388, 726, 1331
Bst4CI ACNGT 7 cut(s) 192, 259, 439, 844, 985, 1078, 1166
Bst6I CTCTTC 7 cut(s) 73, 196, 650, 762, 933, 1135, 1202
BstACI GRCGYC 1 cut(s) 539
BstC8I GCNNGC 1 cut(s) 34
BstENI CCTNNNNNAGG 1 cut(s) 623
BstF5I GGATG 4 cut(s) 446, 636, 720, 912
BstH2I RGCGCY 1 cut(s) 542
BstHHI GCGC 2 cut(s) 417, 541
BstKTI GATC 7 cut(s) 398, 615, 704, 875, 958, 1220, 1316
BstMBI GATC 7 cut(s) 395, 612, 701, 872, 955, 1217, 1313
BstMWI GCNNNNNNNGC 2 cut(s) 357, 974
BstNI CCWGG 5 cut(s) 168, 180, 388, 726, 1331
BstNSI RCATGY 1 cut(s) 1174
BstSCI CCNGG 6 cut(s) 166, 178, 386, 724, 1309, 1329
BstSLI GKGCMC 1 cut(s) 353
BstV1I GCAGC 3 cut(s) 16, 980, 1554
BstX2I RGATCY 3 cut(s) 395, 701, 1313
BstYI RGATCY 3 cut(s) 395, 701, 1313
BsuI GTATCC 1 cut(s) 288
BsuRI GGCC 3 cut(s) 301, 857, 1309
BtgZI GCGATG 1 cut(s) 1299
BtsCI GGATG 4 cut(s) 446, 636, 720, 912
BtsI GCAGTG 1 cut(s) 583
BtsIMutI CAGTG 5 cut(s) 188, 368, 444, 583, 1140
Cac8I GCNNGC 1 cut(s) 34
CfoI GCGC 2 cut(s) 417, 541
Cfr10I RCCGGY 1 cut(s) 16
Cfr13I GGNCC 6 cut(s) 384, 598, 856, 909, 985, 1308
Csp6I GTAC 2 cut(s) 605, 743
CspCI CAANNNNNGTGG 2 cut(s) 576, 611
CviAII CATG 6 cut(s) 209, 400, 825, 1171, 1338, 1421
CviQI GTAC 2 cut(s) 605, 743
DinI GGCGCC 1 cut(s) 540
DpnI GATC 7 cut(s) 397, 614, 703, 874, 957, 1219, 1315
DpnII GATC 7 cut(s) 395, 612, 701, 872, 955, 1217, 1313
Eam1104I CTCTTC 7 cut(s) 73, 196, 650, 762, 933, 1135, 1202
EarI CTCTTC 7 cut(s) 73, 196, 650, 762, 933, 1135, 1202
Eco130I CCWWGG 2 cut(s) 378, 1461
Eco32I GATATC 1 cut(s) 273
Eco47I GGWCC 4 cut(s) 384, 598, 909, 985
Eco57I CTGAAG 2 cut(s) 1116, 1132
Eco88I CYCGRG 1 cut(s) 1303
EcoNI CCTNNNNNAGG 1 cut(s) 623
EcoO109I RGGNCCY 1 cut(s) 384
EcoRII CCWGG 5 cut(s) 166, 178, 386, 724, 1329
EcoRV GATATC 1 cut(s) 273
EcoT14I CCWWGG 2 cut(s) 378, 1461
EgeI GGCGCC 1 cut(s) 540
EheI GGCGCC 1 cut(s) 540
ErhI CCWWGG 2 cut(s) 378, 1461
FaeI CATG 6 cut(s) 212, 403, 828, 1174, 1341, 1424
FaqI GGGAC 2 cut(s) 454, 971
FatI CATG 6 cut(s) 208, 399, 824, 1170, 1337, 1420
FauI CCCGC 1 cut(s) 1131
Fnu4HI GCNGC 3 cut(s) 30, 969, 1543
FokI GGATG 4 cut(s) 433, 643, 707, 899
Fsp4HI GCNGC 3 cut(s) 30, 969, 1543
FspBI CTAG 5 cut(s) 107, 500, 885, 1344, 1582
GlaI GCGC 2 cut(s) 416, 540
GluI GCNGC 3 cut(s) 30, 969, 1543
GsuI CTGGAG 2 cut(s) 260, 840
HaeII RGCGCY 1 cut(s) 542
HaeIII GGCC 3 cut(s) 301, 857, 1309
HapII CCGG 3 cut(s) 17, 916, 1311
HhaI GCGC 2 cut(s) 417, 541
Hin1I GRCGYC 1 cut(s) 539
Hin1II CATG 6 cut(s) 212, 403, 828, 1174, 1341, 1424
Hin6I GCGC 2 cut(s) 415, 539
HinP1I GCGC 2 cut(s) 415, 539
HindIII AAGCTT 1 cut(s) 635
HinfI GANTC 7 cut(s) 86, 834, 881, 1156, 1225, 1232, 1521
HpaII CCGG 3 cut(s) 17, 916, 1311
Hpy166II GTNNAC 1 cut(s) 909
Hpy188I TCNGA 9 cut(s) 85, 294, 549, 654, 668, 1151, 1222, 1246, 1363
Hpy188III TCNNGA 9 cut(s) 239, 393, 526, 838, 1177, 1229, 1303, 1317, 1344
Hpy8I GTNNAC 1 cut(s) 909
Hpy99I CGWCG 2 cut(s) 1170, 1453
HpyAV CCTTC 4 cut(s) 256, 552, 1251, 1386
HpyCH4III ACNGT 7 cut(s) 192, 259, 439, 844, 985, 1078, 1166
HpyCH4V TGCA 6 cut(s) 36, 133, 157, 1253, 1352, 1508
HpyF10VI GCNNNNNNNGC 2 cut(s) 357, 974
Hsp92I GRCGYC 1 cut(s) 539
Hsp92II CATG 6 cut(s) 212, 403, 828, 1174, 1341, 1424
HspAI GCGC 2 cut(s) 415, 539
KasI GGCGCC 1 cut(s) 538
Kzo9I GATC 7 cut(s) 395, 612, 701, 872, 955, 1217, 1313
LguI GCTCTTC 2 cut(s) 933, 1202
LmnI GCTCC 3 cut(s) 821, 974, 999
Lsp1109I GCAGC 3 cut(s) 16, 980, 1554
LweI GCATC 1 cut(s) 1361
MaeI CTAG 5 cut(s) 107, 500, 885, 1344, 1582
MaeIII GTNAC 2 cut(s) 402, 1160
MalI GATC 7 cut(s) 397, 614, 703, 874, 957, 1219, 1315
MbiI CCGCTC 1 cut(s) 1409
MboI GATC 7 cut(s) 395, 612, 701, 872, 955, 1217, 1313
MfeI CAATTG 1 cut(s) 876
MflI RGATCY 3 cut(s) 395, 701, 1313
MhlI GDGCHC 1 cut(s) 353
Mly113I GGCGCC 1 cut(s) 539
MmeI TCCRAC 1 cut(s) 632
MroXI GAANNNNTTC 1 cut(s) 328
MseI TTAA 1 cut(s) 231
MslI CAYNNNNRTG 2 cut(s) 193, 213
MspI CCGG 3 cut(s) 17, 916, 1311
MspR9I CCNGG 6 cut(s) 168, 180, 388, 726, 1311, 1331
MunI CAATTG 1 cut(s) 876
Mva1269I GAATGC 2 cut(s) 36, 127
MvaI CCWGG 5 cut(s) 168, 180, 388, 726, 1331
MwoI GCNNNNNNNGC 2 cut(s) 357, 974
NarI GGCGCC 1 cut(s) 539
NciI CCSGG 1 cut(s) 1311
NdeII GATC 7 cut(s) 395, 612, 701, 872, 955, 1217, 1313
NlaIII CATG 6 cut(s) 212, 403, 828, 1174, 1341, 1424
NlaIV GGNNCC 7 cut(s) 385, 397, 540, 976, 987, 1315, 1413
NmuCI GTSAC 2 cut(s) 402, 1160
NspI RCATGY 1 cut(s) 1174
OliI CACNNNNGTG 1 cut(s) 193
PasI CCCWGGG 1 cut(s) 725
PciSI GCTCTTC 2 cut(s) 933, 1202
PctI GAATGC 2 cut(s) 36, 127
PdmI GAANNNNTTC 1 cut(s) 328
PfeI GAWTC 7 cut(s) 86, 834, 881, 1156, 1225, 1232, 1521
PkrI GCNGC 3 cut(s) 31, 970, 1544
PluTI GGCGCC 1 cut(s) 542
PpuMI RGGWCCY 1 cut(s) 384
Psp5II RGGWCCY 1 cut(s) 384
Psp6I CCWGG 5 cut(s) 166, 178, 386, 724, 1329
PspGI CCWGG 5 cut(s) 166, 178, 386, 724, 1329
PspN4I GGNNCC 7 cut(s) 385, 397, 540, 976, 987, 1315, 1413
PspPI GGNCC 6 cut(s) 384, 598, 856, 909, 985, 1308
PspPPI RGGWCCY 1 cut(s) 384
PsuI RGATCY 3 cut(s) 395, 701, 1313
RsaI GTAC 2 cut(s) 606, 744
RsaNI GTAC 2 cut(s) 605, 743
RseI CAYNNNNRTG 2 cut(s) 193, 213
SapI GCTCTTC 2 cut(s) 933, 1202
SaqAI TTAA 1 cut(s) 231
SatI GCNGC 3 cut(s) 30, 969, 1543
Sau3AI GATC 7 cut(s) 395, 612, 701, 872, 955, 1217, 1313
Sau96I GGNCC 6 cut(s) 384, 598, 856, 909, 985, 1308
ScaI AGTACT 1 cut(s) 744
ScrFI CCNGG 6 cut(s) 168, 180, 388, 726, 1311, 1331
SduI GDGCHC 1 cut(s) 353
SfaNI GCATC 1 cut(s) 1361
SfoI GGCGCC 1 cut(s) 540
SinI GGWCC 4 cut(s) 384, 598, 909, 985
SmiMI CAYNNNNRTG 2 cut(s) 193, 213
SsiI CCGC 3 cut(s) 959, 1138, 1409
SspDI GGCGCC 1 cut(s) 538
SspI AATATT 1 cut(s) 1113
SspMI CTAG 5 cut(s) 107, 500, 885, 1344, 1582
StyD4I CCNGG 6 cut(s) 166, 178, 386, 724, 1309, 1329
StyI CCWWGG 2 cut(s) 378, 1461
TaaI ACNGT 7 cut(s) 192, 259, 439, 844, 985, 1078, 1166
TaqI TCGA 2 cut(s) 140, 284
TatI WGTACW 2 cut(s) 604, 742
TfiI GAWTC 7 cut(s) 86, 834, 881, 1156, 1225, 1232, 1521
Tru1I TTAA 1 cut(s) 231
Tru9I TTAA 1 cut(s) 231
TscAI CASTG 5 cut(s) 195, 368, 444, 583, 1140
TseFI GTSAC 2 cut(s) 402, 1160
TseI GCWGC 3 cut(s) 29, 968, 1542
Tsp45I GTSAC 2 cut(s) 402, 1160
TspDTI ATGAA 6 cut(s) 197, 435, 546, 647, 672, 1326
TspGWI ACGGA 2 cut(s) 448, 1466
TspRI CASTG 5 cut(s) 195, 368, 444, 583, 1140
VpaK11BI GGWCC 4 cut(s) 384, 598, 909, 985
XagI CCTNNNNNAGG 1 cut(s) 623
XapI RAATTY 4 cut(s) 160, 280, 482, 669
XbaI TCTAGA 1 cut(s) 1343
XceI RCATGY 1 cut(s) 1174
XcmI CCANNNNNNNNNTGG 1 cut(s) 1422
XmnI GAANNNNTTC 1 cut(s) 328
XspI CTAG 5 cut(s) 107, 500, 885, 1344, 1582
ZrmI AGTACT 1 cut(s) 744
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.