Rmu_co8406947.1_g000001

NEMP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8406947.1
OG0001753
Physical Location & Seq
Forward (+)
217 .. 1281
1065 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8406947.1_g000001.1.cds

Sequence Viewer

Length: 702 bp
atggcttcttctaagcaggttcaggtcacagcttgtattctatggcttctgttcctcacacttccatgttcttcttccactgaatcactctatcagcattctttggttattggtgaaaatgctactttaggattgtctcatggtatgccagtcggaaaatctcccggttctaagcctggcgcttcggtgttgtgtgaacgagttcttatctatggattgtcaaggcttaaacatccggataagtttggcaatacggtaatgaagcttctcccaactggtcggaagaattcactcgcaattttcgtcttgtcatctgtggttggtttgggatcttttctcctccgctacatcccaggactactaagttcagtactcaccgagcttggaattagtgaggatatgtataatgttttggccatatttcttctggcttttgtttttccggctggagcatcgttgggattctggacagttcttaaaatggccttgaacgaagatggatcaattgattctagcatatctctgtttgttttgtggaccattcgcttcgtagctgttatttttctttcacagagttccagcgatcaactactggcagcaagagctataatggtaactttttttgagaaaataaataacttcagtacttatctatggtcagaaggcacgtatatcataagttgtctcataccaaattcttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NEMP

Protein Analysis

233

Amino Acids

25.29

Weight (kDa)

8.35

Isoelectric Point (pI)

37.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 7
AccIII TCCGGA 1 cut(s) 235
AciI CCGC 1 cut(s) 343
AclWI GGATC 2 cut(s) 337, 508
AcoI YGGCCR 1 cut(s) 414
AcsI RAATTY 2 cut(s) 286, 694
AcuI CTGAAG 1 cut(s) 625
AfaI GTAC 2 cut(s) 372, 646
AgsI TTSAA 1 cut(s) 490
AjnI CCWGG 2 cut(s) 175, 352
AluBI AGCT 5 cut(s) 32, 265, 382, 554, 605
AluI AGCT 5 cut(s) 32, 265, 382, 554, 605
Alw26I GTCTC 2 cut(s) 141, 689
AlwI GGATC 2 cut(s) 337, 508
Aor13HI TCCGGA 1 cut(s) 235
AoxI GGCC 2 cut(s) 414, 483
ApeKI GCWGC 1 cut(s) 596
ApoI RAATTY 2 cut(s) 286, 694
Asp700I GAANNNNTTC 1 cut(s) 201
AspLEI GCGC 1 cut(s) 182
AspS9I GGNCC 1 cut(s) 537
AsuC2I CCSGG 1 cut(s) 165
AsuHPI GGTGA 2 cut(s) 125, 367
AvaII GGWCC 1 cut(s) 537
BalI TGGCCA 1 cut(s) 416
BarI GAAGNNNNNNTAC 2 cut(s) 249, 281
BbvI GCAGC 1 cut(s) 608
BccI CCATC 1 cut(s) 491
BciT130I CCWGG 2 cut(s) 177, 354
BcnI CCSGG 1 cut(s) 165
BcoDI GTCTC 2 cut(s) 141, 689
BfaI CTAG 1 cut(s) 513
BfoI RGCGCY 1 cut(s) 183
BfuAI ACCTGC 1 cut(s) 7
BisI GCNGC 1 cut(s) 597
BlsI GCNGC 1 cut(s) 598
BmcAI AGTACT 2 cut(s) 372, 646
Bme1390I CCNGG 3 cut(s) 165, 177, 354
Bme18I GGWCC 1 cut(s) 537
BmgT120I GGNCC 1 cut(s) 537
BmrFI CCNGG 3 cut(s) 165, 177, 354
BmsI GCATC 1 cut(s) 461
BpmI CTGGAG 1 cut(s) 468
BpuMI CCSGG 1 cut(s) 165
BsaAI YACGTR 1 cut(s) 669
BsaJI CCNNGG 1 cut(s) 352
BsaWI WCCGGW 1 cut(s) 235
Bse1I ACTGG 3 cut(s) 149, 280, 597
BseAI TCCGGA 1 cut(s) 235
BseBI CCWGG 2 cut(s) 177, 354
BseDI CCNNGG 1 cut(s) 352
BseGI GGATG 2 cut(s) 232, 348
BseNI ACTGG 3 cut(s) 149, 280, 597
BseRI GAGGAG 1 cut(s) 329
BseXI GCAGC 1 cut(s) 608
BshFI GGCC 2 cut(s) 416, 485
BsiSI CCGG 3 cut(s) 165, 236, 443
BsmAI GTCTC 2 cut(s) 141, 689
BsmI GAATGC 1 cut(s) 97
BsnI GGCC 2 cut(s) 416, 485
Bsp13I TCCGGA 1 cut(s) 235
Bsp143I GATC 3 cut(s) 329, 500, 583
BspACI CCGC 1 cut(s) 343
BspANI GGCC 2 cut(s) 416, 485
BspEI TCCGGA 1 cut(s) 235
BspMI ACCTGC 1 cut(s) 7
BspPI GGATC 2 cut(s) 337, 508
BsrI ACTGG 3 cut(s) 149, 280, 597
BssECI CCNNGG 1 cut(s) 352
BssMI GATC 3 cut(s) 329, 500, 583
Bst2UI CCWGG 2 cut(s) 177, 354
Bst4CI ACNGT 2 cut(s) 256, 472
BstBAI YACGTR 1 cut(s) 669
BstDEI CTNAG 3 cut(s) 12, 171, 362
BstF5I GGATG 2 cut(s) 232, 348
BstH2I RGCGCY 1 cut(s) 183
BstHHI GCGC 1 cut(s) 182
BstKTI GATC 3 cut(s) 332, 503, 586
BstMAI GTCTC 2 cut(s) 141, 689
BstMBI GATC 3 cut(s) 329, 500, 583
BstMWI GCNNNNNNNGC 1 cut(s) 602
BstNI CCWGG 2 cut(s) 177, 354
BstSCI CCNGG 3 cut(s) 163, 175, 352
BstV1I GCAGC 1 cut(s) 608
BstX2I RGATCY 1 cut(s) 329
BstYI RGATCY 1 cut(s) 329
BsuRI GGCC 2 cut(s) 416, 485
BtsCI GGATG 2 cut(s) 232, 348
BtsIMutI CAGTG 1 cut(s) 78
BveI ACCTGC 1 cut(s) 7
CfoI GCGC 1 cut(s) 182
Cfr13I GGNCC 1 cut(s) 537
Csp6I GTAC 2 cut(s) 371, 645
CviAII CATG 2 cut(s) 66, 140
CviQI GTAC 2 cut(s) 371, 645
DdeI CTNAG 3 cut(s) 12, 171, 362
DpnI GATC 3 cut(s) 331, 502, 585
DpnII GATC 3 cut(s) 329, 500, 583
EaeI YGGCCR 1 cut(s) 414
Eco47I GGWCC 1 cut(s) 537
Eco57I CTGAAG 1 cut(s) 625
EcoRI GAATTC 1 cut(s) 286
EcoRII CCWGG 2 cut(s) 175, 352
FaeI CATG 2 cut(s) 69, 143
FatI CATG 2 cut(s) 65, 139
Fnu4HI GCNGC 1 cut(s) 597
FokI GGATG 2 cut(s) 219, 335
Fsp4HI GCNGC 1 cut(s) 597
FspBI CTAG 1 cut(s) 513
GlaI GCGC 1 cut(s) 181
GluI GCNGC 1 cut(s) 597
GsuI CTGGAG 1 cut(s) 468
HaeII RGCGCY 1 cut(s) 183
HaeIII GGCC 2 cut(s) 416, 485
HapII CCGG 3 cut(s) 165, 236, 443
HhaI GCGC 1 cut(s) 182
Hin1II CATG 2 cut(s) 69, 143
Hin6I GCGC 1 cut(s) 180
HinP1I GCGC 1 cut(s) 180
HindIII AAGCTT 1 cut(s) 263
HinfI GANTC 3 cut(s) 83, 462, 509
HpaII CCGG 3 cut(s) 165, 236, 443
HphI GGTGA 2 cut(s) 125, 367
Hpy166II GTNNAC 2 cut(s) 197, 537
Hpy188I TCNGA 3 cut(s) 155, 282, 661
Hpy188III TCNNGA 3 cut(s) 236, 466, 699
Hpy8I GTNNAC 2 cut(s) 197, 537
HpyAV CCTTC 1 cut(s) 656
HpyCH4III ACNGT 2 cut(s) 256, 472
HpyCH4IV ACGT 1 cut(s) 668
HpyF10VI GCNNNNNNNGC 1 cut(s) 602
HpyF3I CTNAG 3 cut(s) 12, 171, 362
HpySE526I ACGT 1 cut(s) 668
Hsp92II CATG 2 cut(s) 69, 143
HspAI GCGC 1 cut(s) 180
Kpn2I TCCGGA 1 cut(s) 235
Kzo9I GATC 3 cut(s) 329, 500, 583
LmnI GCTCC 1 cut(s) 449
Lsp1109I GCAGC 1 cut(s) 608
LweI GCATC 1 cut(s) 461
MaeI CTAG 1 cut(s) 513
MaeII ACGT 1 cut(s) 668
MaeIII GTNAC 2 cut(s) 25, 613
MalI GATC 3 cut(s) 331, 502, 585
MboI GATC 3 cut(s) 329, 500, 583
MboII GAAGA 5 cut(s) 63, 66, 295, 416, 506
MfeI CAATTG 1 cut(s) 504
MflI RGATCY 1 cut(s) 329
MlsI TGGCCA 1 cut(s) 416
MluCI AATT 5 cut(s) 286, 297, 387, 504, 694
MluNI TGGCCA 1 cut(s) 416
MmeI TCCRAC 2 cut(s) 133, 260
MnlI CCTC 3 cut(s) 65, 350, 388
Mox20I TGGCCA 1 cut(s) 416
MroI TCCGGA 1 cut(s) 235
MroXI GAANNNNTTC 1 cut(s) 201
MscI TGGCCA 1 cut(s) 416
MseI TTAA 2 cut(s) 228, 477
MslI CAYNNNNRTG 1 cut(s) 64
Msp20I TGGCCA 1 cut(s) 416
MspI CCGG 3 cut(s) 165, 236, 443
MspR9I CCNGG 3 cut(s) 165, 177, 354
MunI CAATTG 1 cut(s) 504
Mva1269I GAATGC 1 cut(s) 97
MvaI CCWGG 2 cut(s) 177, 354
MwoI GCNNNNNNNGC 1 cut(s) 602
NciI CCSGG 1 cut(s) 165
NdeII GATC 3 cut(s) 329, 500, 583
NlaIII CATG 2 cut(s) 69, 143
NmuCI GTSAC 1 cut(s) 25
PcsI WCGNNNNNNNCGW 1 cut(s) 300
PctI GAATGC 1 cut(s) 97
PdmI GAANNNNTTC 1 cut(s) 201
PfeI GAWTC 3 cut(s) 83, 462, 509
PkrI GCNGC 1 cut(s) 598
Ppu21I YACGTR 1 cut(s) 669
Psp6I CCWGG 2 cut(s) 175, 352
PspGI CCWGG 2 cut(s) 175, 352
PspPI GGNCC 1 cut(s) 537
PsuI RGATCY 1 cut(s) 329
RsaI GTAC 2 cut(s) 372, 646
RsaNI GTAC 2 cut(s) 371, 645
RseI CAYNNNNRTG 1 cut(s) 64
SaqAI TTAA 2 cut(s) 228, 477
SatI GCNGC 1 cut(s) 597
Sau3AI GATC 3 cut(s) 329, 500, 583
Sau96I GGNCC 1 cut(s) 537
ScaI AGTACT 2 cut(s) 372, 646
ScrFI CCNGG 3 cut(s) 165, 177, 354
SetI ASST 8 cut(s) 21, 27, 34, 267, 384, 556, 607, 671
SfaNI GCATC 1 cut(s) 461
SinI GGWCC 1 cut(s) 537
SmiMI CAYNNNNRTG 1 cut(s) 64
Sse9I AATT 5 cut(s) 286, 297, 387, 504, 694
SsiI CCGC 1 cut(s) 343
SspMI CTAG 1 cut(s) 513
StyD4I CCNGG 3 cut(s) 163, 175, 352
TaaI ACNGT 2 cut(s) 256, 472
TaiI ACGT 1 cut(s) 671
TasI AATT 5 cut(s) 286, 297, 387, 504, 694
TatI WGTACW 2 cut(s) 370, 644
TfiI GAWTC 3 cut(s) 83, 462, 509
Tru1I TTAA 2 cut(s) 228, 477
Tru9I TTAA 2 cut(s) 228, 477
TscAI CASTG 1 cut(s) 85
TseFI GTSAC 1 cut(s) 25
TseI GCWGC 1 cut(s) 596
Tsp45I GTSAC 1 cut(s) 25
TspDTI ATGAA 1 cut(s) 275
TspRI CASTG 1 cut(s) 85
VpaK11BI GGWCC 1 cut(s) 537
XapI RAATTY 2 cut(s) 286, 694
XcmI CCANNNNNNNNNTGG 1 cut(s) 424
XmnI GAANNNNTTC 1 cut(s) 201
XspI CTAG 1 cut(s) 513
ZrmI AGTACT 2 cut(s) 372, 646
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.