RLG00000017659

NEMP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0001753
Physical Location & Seq
Reverse (-)
19521170 .. 19525060
3891 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017659

Sequence Viewer

Length: 1581 bp
ATGGCTTCTTCTAAGCCGGTTCGGGTCGCTGCGTGCCTTCTATGGCTTCTGTTCCTCACGCTTCCCTCTTCTTCTTCCTCTGAATCGCCGACTGAGTATTCTCTAGTAATTGGTGAGAATGCTACTTTGCAATTATCGAATGGTGTGCTGGTGCAAAATTCTCCTGGTGCTAAGCCTGGAACTTCAGTGTTGTGTGGAAGAGTTCATGTACGTGGATTGTCAAGGCTTAAATATCTGGAGAAGTTTGCCGACACGGTGAAGGTGAAGATATCAACAAATTCGAGCTTTCGGATATGGCCTTTTGAGGTTTGCTTCCACAGAAATACTTCTCGTGGGATAGGAATGTGCCCTCACAGCCAGTGGGAGAAAGTTTCCAAGGGGTCCTGGTCTGGATCCATGTCACCTTATGAGCGCAAACTCTTAGATATACGGACAGTGGGTTCATCCTTAGAGAGTTTTGAGGTGTCCCTTGAAGAAGAATTTTTTATATCTCGCCTAGTATTTTTTGTGTCTGGTGTAATCTTGATGAGTTTGGCGCCTTCTCTGAGCAATTCATTGGTATTCTATTACGGCAGTGGAATGGCAATAGGGGTGGTCCTTGTACTATTGATCATCCTTTTTCAGGGGATGAAGCTTCTCCCAACAGGTCGGAAGAGTTCGCTCGGAATTTTTTTGTTTTCATCTGTGGTTGGTTTGGGATCTTTTCTCCTCAGCTACATCCCTGGGCTATTGCGTTCAGTACTAACAGAGCTTGGAATTAGCGAAGAGATGTATAATCCTTTGGCTATATTTCTTCTGGCTTTTATTTTTCTGGCTGGAGCATGGTTGGGATTCTGGACAGTTCGTAAAATGGCCCTGAGAGAAGATGGATCAATTGATTCTAGCACATCTCTGTTTGTTTTGTGGACCATCCGGTTTGTAGCTCTTCTTCTGCTTTCACAGAGTTCTGGTGATCCGCTACTGGCAGCAGGAGCCATAATGGTCCCCTTGCTCCCTTTTATAGTGAGGAAAATTGCTATAATGTTTGGAGTTGTACTCTCTTTTATGAGGAAAATAACCATAGCATTTGGAACAATAGTCTCTTTTATAGCGAGGACAATCGCTATAATATTTGGAGGTGTAATCTCTTCAGTGCGGGAAATCTTCAGATGGATTCGTCACCGTCGCATGTACAGGAAATTGTTGAAATCACCCAAAAAGAAGAAGAGCAGAAGATCAGAGATTCCTGATTCTCCACCTTCTGAATATGCACCTGATGAGGTCATCAACGAGTTTCAAAGCAATGACGACTATAAGTTCCCGAGGCCCGGATCCAGAAACTCTACCCTGGCTTCATGTTCTAGAAGTGCATCACCATCAGAAAGGTTATACCCTTCTTATATCCACACTACACCTGAGAGGAGAGCGGTGCCAAGACATGAGTGGGAAAGTATCACCAGACACACGACGGAGAAAGCCCTGGAAGAACTTGCTTCTTCACCTGATTTCAGTAAGTGGGTAGTTGCAAATGCTGATAGAATCAGTATCACCCCACTCAGCAGCAGAAAGACTGATGAGCAACGCCGTAGCTGGTTCTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NEMP

Protein Analysis

527

Amino Acids

58.75

Weight (kDa)

9.87

Isoelectric Point (pI)

54.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NEMP PF10225 154 - 338 3.4e-34 NEMP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 535, 1408
AccBSI CCGCTC 1 cut(s) 1406
AciI CCGC 3 cut(s) 956, 1135, 1406
AclWI GGATC 7 cut(s) 387, 400, 706, 877, 947, 1305, 1318
AcsI RAATTY 4 cut(s) 157, 277, 479, 666
AcuI CTGAAG 3 cut(s) 168, 1113, 1129
AcyI GRCGYC 1 cut(s) 536
AfaI GTAC 5 cut(s) 210, 603, 741, 1035, 1172
AfiI CCNNNNNNNGG 2 cut(s) 622, 1307
AgsI TTSAA 3 cut(s) 473, 1186, 1277
AjnI CCWGG 6 cut(s) 163, 175, 383, 721, 1326, 1458
AloI GAACNNNNNNTCC 2 cut(s) 424, 456
AluBI AGCT 6 cut(s) 285, 634, 714, 751, 923, 1569
AluI AGCT 6 cut(s) 285, 634, 714, 751, 923, 1569
Alw26I GTCTC 1 cut(s) 1084
AlwI GGATC 7 cut(s) 387, 400, 706, 877, 947, 1305, 1318
Ama87I CYCGRG 1 cut(s) 1300
AoxI GGCC 3 cut(s) 296, 852, 1304
ApeKI GCWGC 3 cut(s) 29, 965, 1539
ApoI RAATTY 4 cut(s) 157, 277, 479, 666
Asp700I GAANNNNTTC 1 cut(s) 325
AspLEI GCGC 2 cut(s) 414, 538
AspS9I GGNCC 6 cut(s) 381, 595, 853, 906, 982, 1305
AsuC2I CCSGG 1 cut(s) 1308
AvaI CYCGRG 1 cut(s) 1300
AvaII GGWCC 4 cut(s) 381, 595, 906, 982
BaeGI GKGCMC 1 cut(s) 350
BamHI GGATCC 2 cut(s) 392, 1310
BanI GGYRCC 2 cut(s) 535, 1408
BarI GAAGNNNNNNTAC 2 cut(s) 912, 944
BauI CACGAG 1 cut(s) 330
BbvCI CCTCAGC 1 cut(s) 710
BbvI GCAGC 3 cut(s) 16, 977, 1551
BccI CCATC 4 cut(s) 860, 917, 1143, 1363
BceAI ACGGC 2 cut(s) 586, 1548
BcgI CGANNNNNNTGC 2 cut(s) 127, 161
BciT130I CCWGG 6 cut(s) 165, 177, 385, 723, 1328, 1460
BclI TGATCA 1 cut(s) 609
BcnI CCSGG 1 cut(s) 1308
BcoDI GTCTC 1 cut(s) 1084
BfaI CTAG 5 cut(s) 104, 497, 882, 1341, 1579
BfoI RGCGCY 1 cut(s) 539
BisI GCNGC 3 cut(s) 30, 966, 1540
BlpI GCTNAGC 1 cut(s) 171
BlsI GCNGC 3 cut(s) 31, 967, 1541
BmcAI AGTACT 1 cut(s) 741
Bme1390I CCNGG 7 cut(s) 165, 177, 385, 723, 1308, 1328, 1460
Bme18I GGWCC 4 cut(s) 381, 595, 906, 982
BmeT110I CYCGRG 1 cut(s) 1300
BmgT120I GGNCC 6 cut(s) 381, 595, 853, 906, 982, 1305
BmiI GGNNCC 7 cut(s) 382, 394, 537, 973, 984, 1312, 1410
BmrFI CCNGG 7 cut(s) 165, 177, 385, 723, 1308, 1328, 1460
BmsI GCATC 1 cut(s) 1358
BplI GAGNNNNNCTC 2 cut(s) 1020, 1052
BpmI CTGGAG 2 cut(s) 257, 837
Bpu10I CCTNAGC 1 cut(s) 710
Bpu1102I GCTNAGC 1 cut(s) 171
BpuMI CCSGG 1 cut(s) 1308
BsaAI YACGTR 1 cut(s) 212
BsaHI GRCGYC 1 cut(s) 536
BsaJI CCNNGG 6 cut(s) 375, 721, 722, 1301, 1326, 1458
BsaWI WCCGGW 1 cut(s) 912
Bsc4I CCNNNNNNNGG 2 cut(s) 622, 1307
Bse118I RCCGGY 1 cut(s) 16
Bse1I ACTGG 2 cut(s) 358, 966
Bse3DI GCAATG 1 cut(s) 1288
BseBI CCWGG 6 cut(s) 165, 177, 385, 723, 1328, 1460
BseDI CCNNGG 6 cut(s) 375, 721, 722, 1301, 1326, 1458
BseGI GGATG 5 cut(s) 443, 612, 633, 717, 909
BseLI CCNNNNNNNGG 2 cut(s) 622, 1307
BseMI GCAATG 1 cut(s) 1288
BseMII CTCAG 6 cut(s) 84, 536, 724, 848, 1386, 1549
BseNI ACTGG 2 cut(s) 358, 966
BseRI GAGGAG 2 cut(s) 698, 1414
BseSI GKGCMC 1 cut(s) 350
BseXI GCAGC 3 cut(s) 16, 977, 1551
BshFI GGCC 3 cut(s) 298, 854, 1306
BshNI GGYRCC 2 cut(s) 535, 1408
BsiHKCI CYCGRG 1 cut(s) 1300
BsiSI CCGG 3 cut(s) 17, 913, 1308
BslFI GGGAC 2 cut(s) 451, 968
BslI CCNNNNNNNGG 2 cut(s) 622, 1307
BsmAI GTCTC 1 cut(s) 1084
BsmFI GGGAC 2 cut(s) 451, 968
BsmI GAATGC 1 cut(s) 124
BsnI GGCC 3 cut(s) 298, 854, 1306
BsoBI CYCGRG 1 cut(s) 1300
Bsp1286I GDGCHC 1 cut(s) 350
Bsp1407I TGTACA 1 cut(s) 1170
Bsp143I GATC 7 cut(s) 392, 609, 698, 869, 952, 1214, 1310
Bsp1720I GCTNAGC 1 cut(s) 171
BspACI CCGC 3 cut(s) 956, 1135, 1406
BspANI GGCC 3 cut(s) 298, 854, 1306
BspCNI CTCAG 6 cut(s) 85, 537, 723, 849, 1387, 1548
BspLI GGNNCC 7 cut(s) 382, 394, 537, 973, 984, 1312, 1410
BspPI GGATC 7 cut(s) 387, 400, 706, 877, 947, 1305, 1318
BspQI GCTCTTC 2 cut(s) 930, 1199
BspT107I GGYRCC 2 cut(s) 535, 1408
BsrBI CCGCTC 1 cut(s) 1406
BsrDI GCAATG 1 cut(s) 1288
BsrFI RCCGGY 1 cut(s) 16
BsrGI TGTACA 1 cut(s) 1170
BsrI ACTGG 2 cut(s) 358, 966
BssAI RCCGGY 1 cut(s) 16
BssECI CCNNGG 6 cut(s) 375, 721, 722, 1301, 1326, 1458
BssMI GATC 7 cut(s) 392, 609, 698, 869, 952, 1214, 1310
BssNI GRCGYC 1 cut(s) 536
BssSI CACGAG 1 cut(s) 330
BssT1I CCWWGG 1 cut(s) 375
Bst2BI CACGAG 1 cut(s) 330
Bst2UI CCWGG 6 cut(s) 165, 177, 385, 723, 1328, 1460
Bst4CI ACNGT 4 cut(s) 256, 436, 841, 1163
Bst6I CTCTTC 7 cut(s) 73, 193, 647, 759, 930, 1132, 1199
BstACI GRCGYC 1 cut(s) 536
BstAUI TGTACA 1 cut(s) 1170
BstBAI YACGTR 1 cut(s) 212
BstC8I GCNNGC 1 cut(s) 34
BstENI CCTNNNNNAGG 1 cut(s) 620
BstF5I GGATG 5 cut(s) 443, 612, 633, 717, 909
BstH2I RGCGCY 1 cut(s) 539
BstHHI GCGC 2 cut(s) 414, 538
BstKTI GATC 7 cut(s) 395, 612, 701, 872, 955, 1217, 1313
BstMAI GTCTC 1 cut(s) 1084
BstMBI GATC 7 cut(s) 392, 609, 698, 869, 952, 1214, 1310
BstMWI GCNNNNNNNGC 2 cut(s) 354, 971
BstNI CCWGG 6 cut(s) 165, 177, 385, 723, 1328, 1460
BstNSI RCATGY 1 cut(s) 1171
BstSCI CCNGG 7 cut(s) 163, 175, 383, 721, 1306, 1326, 1458
BstSLI GKGCMC 1 cut(s) 350
BstV1I GCAGC 3 cut(s) 16, 977, 1551
BstX2I RGATCY 3 cut(s) 392, 698, 1310
BstYI RGATCY 3 cut(s) 392, 698, 1310
BsuRI GGCC 3 cut(s) 298, 854, 1306
BtsCI GGATG 5 cut(s) 443, 612, 633, 717, 909
BtsI GCAGTG 1 cut(s) 580
BtsIMutI CAGTG 5 cut(s) 192, 365, 441, 580, 1137
Cac8I GCNNGC 1 cut(s) 34
CfoI GCGC 2 cut(s) 414, 538
Cfr10I RCCGGY 1 cut(s) 16
Cfr13I GGNCC 6 cut(s) 381, 595, 853, 906, 982, 1305
Csp6I GTAC 5 cut(s) 209, 602, 740, 1034, 1171
CspCI CAANNNNNGTGG 2 cut(s) 573, 608
CviAII CATG 6 cut(s) 206, 397, 822, 1168, 1335, 1418
CviQI GTAC 5 cut(s) 209, 602, 740, 1034, 1171
DinI GGCGCC 1 cut(s) 537
DpnI GATC 7 cut(s) 394, 611, 700, 871, 954, 1216, 1312
DpnII GATC 7 cut(s) 392, 609, 698, 869, 952, 1214, 1310
Eam1104I CTCTTC 7 cut(s) 73, 193, 647, 759, 930, 1132, 1199
EarI CTCTTC 7 cut(s) 73, 193, 647, 759, 930, 1132, 1199
Eco130I CCWWGG 1 cut(s) 375
Eco32I GATATC 1 cut(s) 270
Eco47I GGWCC 4 cut(s) 381, 595, 906, 982
Eco57I CTGAAG 3 cut(s) 168, 1113, 1129
Eco88I CYCGRG 1 cut(s) 1300
EcoNI CCTNNNNNAGG 1 cut(s) 620
EcoO109I RGGNCCY 1 cut(s) 381
EcoRII CCWGG 6 cut(s) 163, 175, 383, 721, 1326, 1458
EcoRV GATATC 1 cut(s) 270
EcoT14I CCWWGG 1 cut(s) 375
EgeI GGCGCC 1 cut(s) 537
EheI GGCGCC 1 cut(s) 537
ErhI CCWWGG 1 cut(s) 375
FaeI CATG 6 cut(s) 209, 400, 825, 1171, 1338, 1421
FaqI GGGAC 2 cut(s) 451, 968
FatI CATG 6 cut(s) 205, 396, 821, 1167, 1334, 1417
FauI CCCGC 1 cut(s) 1128
FbaI TGATCA 1 cut(s) 609
Fnu4HI GCNGC 3 cut(s) 30, 966, 1540
FokI GGATG 5 cut(s) 430, 599, 640, 704, 896
Fsp4HI GCNGC 3 cut(s) 30, 966, 1540
FspBI CTAG 5 cut(s) 104, 497, 882, 1341, 1579
GlaI GCGC 2 cut(s) 413, 537
GluI GCNGC 3 cut(s) 30, 966, 1540
GsuI CTGGAG 2 cut(s) 257, 837
HaeII RGCGCY 1 cut(s) 539
HaeIII GGCC 3 cut(s) 298, 854, 1306
HapII CCGG 3 cut(s) 17, 913, 1308
HhaI GCGC 2 cut(s) 414, 538
Hin1I GRCGYC 1 cut(s) 536
Hin1II CATG 6 cut(s) 209, 400, 825, 1171, 1338, 1421
Hin6I GCGC 2 cut(s) 412, 536
HinP1I GCGC 2 cut(s) 412, 536
HindIII AAGCTT 1 cut(s) 632
HinfI GANTC 7 cut(s) 83, 831, 878, 1153, 1222, 1229, 1518
HpaII CCGG 3 cut(s) 17, 913, 1308
Hpy166II GTNNAC 1 cut(s) 906
Hpy188I TCNGA 9 cut(s) 82, 291, 546, 651, 665, 1148, 1219, 1243, 1360
Hpy188III TCNNGA 8 cut(s) 236, 390, 523, 835, 1226, 1300, 1314, 1341
Hpy8I GTNNAC 1 cut(s) 906
Hpy99I CGWCG 2 cut(s) 1167, 1450
HpyAV CCTTC 5 cut(s) 47, 253, 549, 1248, 1383
HpyCH4III ACNGT 4 cut(s) 256, 436, 841, 1163
HpyCH4IV ACGT 1 cut(s) 211
HpyCH4V TGCA 5 cut(s) 130, 154, 1250, 1349, 1505
HpyF10VI GCNNNNNNNGC 2 cut(s) 354, 971
HpySE526I ACGT 1 cut(s) 211
Hsp92I GRCGYC 1 cut(s) 536
Hsp92II CATG 6 cut(s) 209, 400, 825, 1171, 1338, 1421
HspAI GCGC 2 cut(s) 412, 536
KasI GGCGCC 1 cut(s) 535
Ksp22I TGATCA 1 cut(s) 609
Kzo9I GATC 7 cut(s) 392, 609, 698, 869, 952, 1214, 1310
LguI GCTCTTC 2 cut(s) 930, 1199
LmnI GCTCC 3 cut(s) 818, 971, 996
Lsp1109I GCAGC 3 cut(s) 16, 977, 1551
LweI GCATC 1 cut(s) 1358
MaeI CTAG 5 cut(s) 104, 497, 882, 1341, 1579
MaeII ACGT 1 cut(s) 211
MaeIII GTNAC 2 cut(s) 399, 1157
MalI GATC 7 cut(s) 394, 611, 700, 871, 954, 1216, 1312
MbiI CCGCTC 1 cut(s) 1406
MboI GATC 7 cut(s) 392, 609, 698, 869, 952, 1214, 1310
MfeI CAATTG 1 cut(s) 873
MflI RGATCY 3 cut(s) 392, 698, 1310
MhlI GDGCHC 1 cut(s) 350
Mly113I GGCGCC 1 cut(s) 536
MmeI TCCRAC 1 cut(s) 629
MroXI GAANNNNTTC 1 cut(s) 325
MseI TTAA 1 cut(s) 228
MslI CAYNNNNRTG 1 cut(s) 210
MspI CCGG 3 cut(s) 17, 913, 1308
MspR9I CCNGG 7 cut(s) 165, 177, 385, 723, 1308, 1328, 1460
MunI CAATTG 1 cut(s) 873
Mva1269I GAATGC 1 cut(s) 124
MvaI CCWGG 6 cut(s) 165, 177, 385, 723, 1328, 1460
MwoI GCNNNNNNNGC 2 cut(s) 354, 971
NarI GGCGCC 1 cut(s) 536
NciI CCSGG 1 cut(s) 1308
NdeII GATC 7 cut(s) 392, 609, 698, 869, 952, 1214, 1310
NlaIII CATG 6 cut(s) 209, 400, 825, 1171, 1338, 1421
NlaIV GGNNCC 7 cut(s) 382, 394, 537, 973, 984, 1312, 1410
NmuCI GTSAC 2 cut(s) 399, 1157
NspI RCATGY 1 cut(s) 1171
PasI CCCWGGG 1 cut(s) 722
PciSI GCTCTTC 2 cut(s) 930, 1199
PctI GAATGC 1 cut(s) 124
PdmI GAANNNNTTC 1 cut(s) 325
PfeI GAWTC 7 cut(s) 83, 831, 878, 1153, 1222, 1229, 1518
PkrI GCNGC 3 cut(s) 31, 967, 1541
PluTI GGCGCC 1 cut(s) 539
Ppu21I YACGTR 1 cut(s) 212
PpuMI RGGWCCY 1 cut(s) 381
Psp5II RGGWCCY 1 cut(s) 381
Psp6I CCWGG 6 cut(s) 163, 175, 383, 721, 1326, 1458
PspGI CCWGG 6 cut(s) 163, 175, 383, 721, 1326, 1458
PspN4I GGNNCC 7 cut(s) 382, 394, 537, 973, 984, 1312, 1410
PspPI GGNCC 6 cut(s) 381, 595, 853, 906, 982, 1305
PspPPI RGGWCCY 1 cut(s) 381
PsuI RGATCY 3 cut(s) 392, 698, 1310
RsaI GTAC 5 cut(s) 210, 603, 741, 1035, 1172
RsaNI GTAC 5 cut(s) 209, 602, 740, 1034, 1171
RseI CAYNNNNRTG 1 cut(s) 210
SapI GCTCTTC 2 cut(s) 930, 1199
SaqAI TTAA 1 cut(s) 228
SatI GCNGC 3 cut(s) 30, 966, 1540
Sau3AI GATC 7 cut(s) 392, 609, 698, 869, 952, 1214, 1310
Sau96I GGNCC 6 cut(s) 381, 595, 853, 906, 982, 1305
ScaI AGTACT 1 cut(s) 741
ScrFI CCNGG 7 cut(s) 165, 177, 385, 723, 1308, 1328, 1460
SduI GDGCHC 1 cut(s) 350
SfaNI GCATC 1 cut(s) 1358
SfoI GGCGCC 1 cut(s) 537
SinI GGWCC 4 cut(s) 381, 595, 906, 982
SmiMI CAYNNNNRTG 1 cut(s) 210
SsiI CCGC 3 cut(s) 956, 1135, 1406
SspDI GGCGCC 1 cut(s) 535
SspI AATATT 1 cut(s) 1110
SspMI CTAG 5 cut(s) 104, 497, 882, 1341, 1579
StyD4I CCNGG 7 cut(s) 163, 175, 383, 721, 1306, 1326, 1458
StyI CCWWGG 1 cut(s) 375
TaaI ACNGT 4 cut(s) 256, 436, 841, 1163
TaiI ACGT 1 cut(s) 214
TaqI TCGA 2 cut(s) 137, 281
TatI WGTACW 4 cut(s) 601, 739, 1033, 1170
TfiI GAWTC 7 cut(s) 83, 831, 878, 1153, 1222, 1229, 1518
Tru1I TTAA 1 cut(s) 228
Tru9I TTAA 1 cut(s) 228
TscAI CASTG 5 cut(s) 192, 365, 441, 580, 1137
TseFI GTSAC 2 cut(s) 399, 1157
TseI GCWGC 3 cut(s) 29, 965, 1539
Tsp45I GTSAC 2 cut(s) 399, 1157
TspDTI ATGAA 6 cut(s) 194, 432, 543, 644, 669, 1323
TspGWI ACGGA 2 cut(s) 445, 1463
TspRI CASTG 5 cut(s) 192, 365, 441, 580, 1137
VpaK11BI GGWCC 4 cut(s) 381, 595, 906, 982
XagI CCTNNNNNAGG 1 cut(s) 620
XapI RAATTY 4 cut(s) 157, 277, 479, 666
XbaI TCTAGA 1 cut(s) 1340
XceI RCATGY 1 cut(s) 1171
XcmI CCANNNNNNNNNTGG 1 cut(s) 1419
XmnI GAANNNNTTC 1 cut(s) 325
XspI CTAG 5 cut(s) 104, 497, 882, 1341, 1579
ZrmI AGTACT 1 cut(s) 741
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.