RchiOBHm_Chr5g0059801

NEMP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0001753
Physical Location & Seq
Forward (+)
64892421 .. 64893044
624 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ33638

Sequence Viewer

Length: 624 bp
ATGGCTTCTTCTAAGCAGGTTCAGGTCACAGCTTGTATTCTATGGCTTCTGTTCCTCACACTTCCATGTTCTTCTTCCACTGAATCACTCTATCAGCATTCTTTGGTTGTTGGTGAAAATGCTACCTTAGGATTGTCTCATGGTATGCCAGTCGGAAAATCTCCAAGTTCTAAGCCTGGCGCTTCGGTGTTGTGTGAACGAGTTCTTGTCTATGGATTGTCAAGGCTTAAACATCTGGATAAGTTTGCCAATACAGTGAAGGTGAAGATATCAACAACATCAAGATTTCAGACTTTTGAGGTTTGTTTCCATAGAAATACGTCCCGTGGGATAGGAATGTGCCCTGACAGCCAGTGGGAGAAAGTTTCCAAGGGGTCCTGGTCTGGATCCATGTCACCTTTTGAGCACAAACTCTTAGCTATACGAACAGCTGGTTCATCCTTACAGAATTTTGAAGTGTTCATTAAAGAAGAATTTTTCTTATCTCGCTTAATATTTTTTGTATCTGGCATAGTCCTGATGACCTTGGCGCCTTATCTGAGTAACTCATTAGTGTTTTATTACAGCAGCGGAATGACAATAGGGGTGGTTCTTGTAATATTGATTTGGACAGTACTTGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NEMP

Protein Analysis

207

Amino Acids

22.8

Weight (kDa)

9.3

Isoelectric Point (pI)

38.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NEMP PF10225 155 - 205 2.9e-12 NEMP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 7
AccB1I GGYRCC 1 cut(s) 529
AciI CCGC 1 cut(s) 570
AclWI GGATC 2 cut(s) 381, 394
AcsI RAATTY 2 cut(s) 448, 473
AcyI GRCGYC 1 cut(s) 530
AfaI GTAC 1 cut(s) 615
AgsI TTSAA 1 cut(s) 455
AjnI CCWGG 2 cut(s) 175, 377
AluBI AGCT 3 cut(s) 32, 419, 431
AluI AGCT 3 cut(s) 32, 419, 431
Alw21I GWGCWC 1 cut(s) 408
Alw26I GTCTC 1 cut(s) 141
AlwI GGATC 2 cut(s) 381, 394
ApeKI GCWGC 1 cut(s) 567
ApoI RAATTY 2 cut(s) 448, 473
Asp700I GAANNNNTTC 1 cut(s) 201
AspLEI GCGC 2 cut(s) 182, 532
AspS9I GGNCC 1 cut(s) 375
AsuHPI GGTGA 3 cut(s) 125, 274, 387
AvaII GGWCC 1 cut(s) 375
AxyI CCTNAGG 1 cut(s) 127
BaeGI GKGCMC 1 cut(s) 344
BamHI GGATCC 1 cut(s) 386
BanI GGYRCC 1 cut(s) 529
Bbv12I GWGCWC 1 cut(s) 408
BbvI GCAGC 1 cut(s) 579
BciT130I CCWGG 2 cut(s) 177, 379
BcoDI GTCTC 1 cut(s) 141
BfoI RGCGCY 2 cut(s) 183, 533
BfuAI ACCTGC 1 cut(s) 7
BisI GCNGC 1 cut(s) 568
BlsI GCNGC 1 cut(s) 569
BmcAI AGTACT 1 cut(s) 615
Bme1390I CCNGG 2 cut(s) 177, 379
Bme18I GGWCC 1 cut(s) 375
BmgT120I GGNCC 1 cut(s) 375
BmiI GGNNCC 3 cut(s) 376, 388, 531
BmrFI CCNGG 2 cut(s) 177, 379
BsaHI GRCGYC 1 cut(s) 530
BsaJI CCNNGG 3 cut(s) 325, 369, 525
Bse1I ACTGG 2 cut(s) 149, 352
Bse21I CCTNAGG 1 cut(s) 127
BseBI CCWGG 2 cut(s) 177, 379
BseDI CCNNGG 3 cut(s) 325, 369, 525
BseGI GGATG 1 cut(s) 437
BseMII CTCAG 1 cut(s) 530
BseNI ACTGG 2 cut(s) 149, 352
BseSI GKGCMC 1 cut(s) 344
BseXI GCAGC 1 cut(s) 579
BshNI GGYRCC 1 cut(s) 529
BsiHKAI GWGCWC 1 cut(s) 408
BslFI GGGAC 1 cut(s) 307
BsmAI GTCTC 1 cut(s) 141
BsmFI GGGAC 1 cut(s) 307
BsmI GAATGC 1 cut(s) 97
Bsp1286I GDGCHC 2 cut(s) 344, 408
Bsp143I GATC 1 cut(s) 386
BspACI CCGC 1 cut(s) 570
BspCNI CTCAG 1 cut(s) 531
BspLI GGNNCC 3 cut(s) 376, 388, 531
BspMI ACCTGC 1 cut(s) 7
BspPI GGATC 2 cut(s) 381, 394
BspT107I GGYRCC 1 cut(s) 529
BsrI ACTGG 2 cut(s) 149, 352
BssECI CCNNGG 3 cut(s) 325, 369, 525
BssMI GATC 1 cut(s) 386
BssNI GRCGYC 1 cut(s) 530
BssT1I CCWWGG 2 cut(s) 369, 525
Bst2UI CCWGG 2 cut(s) 177, 379
Bst4CI ACNGT 2 cut(s) 256, 613
BstACI GRCGYC 1 cut(s) 530
BstDEI CTNAG 5 cut(s) 12, 127, 171, 415, 539
BstDSI CCRYGG 1 cut(s) 325
BstF5I GGATG 1 cut(s) 437
BstH2I RGCGCY 2 cut(s) 183, 533
BstHHI GCGC 2 cut(s) 182, 532
BstKTI GATC 1 cut(s) 389
BstMAI GTCTC 1 cut(s) 141
BstMBI GATC 1 cut(s) 386
BstMWI GCNNNNNNNGC 1 cut(s) 348
BstNI CCWGG 2 cut(s) 177, 379
BstSCI CCNGG 2 cut(s) 175, 377
BstSLI GKGCMC 1 cut(s) 344
BstV1I GCAGC 1 cut(s) 579
BstX2I RGATCY 1 cut(s) 386
BstYI RGATCY 1 cut(s) 386
Bsu36I CCTNAGG 1 cut(s) 127
BtgI CCRYGG 1 cut(s) 325
BtsCI GGATG 1 cut(s) 437
BtsIMutI CAGTG 3 cut(s) 78, 261, 359
BveI ACCTGC 1 cut(s) 7
CfoI GCGC 2 cut(s) 182, 532
Cfr13I GGNCC 1 cut(s) 375
Csp6I GTAC 1 cut(s) 614
CspCI CAANNNNNGTGG 2 cut(s) 567, 602
CviAII CATG 3 cut(s) 66, 140, 391
CviJI RGCY 9 cut(s) 5, 32, 46, 175, 226, 351, 419, 431, 621
CviKI_1 RGCY 9 cut(s) 5, 32, 46, 175, 226, 351, 419, 431, 621
CviQI GTAC 1 cut(s) 614
DdeI CTNAG 5 cut(s) 12, 127, 171, 415, 539
DinI GGCGCC 1 cut(s) 531
DpnI GATC 1 cut(s) 388
DpnII GATC 1 cut(s) 386
Eco130I CCWWGG 2 cut(s) 369, 525
Eco32I GATATC 1 cut(s) 270
Eco47I GGWCC 1 cut(s) 375
Eco81I CCTNAGG 1 cut(s) 127
EcoO109I RGGNCCY 1 cut(s) 375
EcoRII CCWGG 2 cut(s) 175, 377
EcoRV GATATC 1 cut(s) 270
EcoT14I CCWWGG 2 cut(s) 369, 525
EgeI GGCGCC 1 cut(s) 531
EheI GGCGCC 1 cut(s) 531
ErhI CCWWGG 2 cut(s) 369, 525
FaeI CATG 3 cut(s) 69, 143, 394
FaiI YATR 9 cut(s) 43, 67, 141, 146, 213, 312, 392, 422, 512
FaqI GGGAC 1 cut(s) 307
FatI CATG 3 cut(s) 65, 139, 390
Fnu4HI GCNGC 1 cut(s) 568
FokI GGATG 1 cut(s) 424
Fsp4HI GCNGC 1 cut(s) 568
GlaI GCGC 2 cut(s) 181, 531
GluI GCNGC 1 cut(s) 568
HaeII RGCGCY 2 cut(s) 183, 533
HhaI GCGC 2 cut(s) 182, 532
Hin1I GRCGYC 1 cut(s) 530
Hin1II CATG 3 cut(s) 69, 143, 394
Hin6I GCGC 2 cut(s) 180, 530
HinP1I GCGC 2 cut(s) 180, 530
HinfI GANTC 1 cut(s) 83
HphI GGTGA 3 cut(s) 125, 274, 387
Hpy166II GTNNAC 1 cut(s) 197
Hpy188I TCNGA 3 cut(s) 155, 291, 540
Hpy188III TCNNGA 4 cut(s) 236, 282, 384, 517
Hpy8I GTNNAC 1 cut(s) 197
HpyAV CCTTC 1 cut(s) 253
HpyCH4III ACNGT 2 cut(s) 256, 613
HpyCH4IV ACGT 1 cut(s) 320
HpyF10VI GCNNNNNNNGC 1 cut(s) 348
HpyF3I CTNAG 5 cut(s) 12, 127, 171, 415, 539
HpySE526I ACGT 1 cut(s) 320
Hsp92I GRCGYC 1 cut(s) 530
Hsp92II CATG 3 cut(s) 69, 143, 394
HspAI GCGC 2 cut(s) 180, 530
KasI GGCGCC 1 cut(s) 529
Kzo9I GATC 1 cut(s) 386
Lsp1109I GCAGC 1 cut(s) 579
MaeII ACGT 1 cut(s) 320
MaeIII GTNAC 3 cut(s) 25, 393, 542
MalI GATC 1 cut(s) 388
MboI GATC 1 cut(s) 386
MboII GAAGA 4 cut(s) 63, 66, 277, 482
MflI RGATCY 1 cut(s) 386
MhlI GDGCHC 2 cut(s) 344, 408
MluCI AATT 2 cut(s) 448, 473
Mly113I GGCGCC 1 cut(s) 530
MmeI TCCRAC 1 cut(s) 133
MnlI CCTC 2 cut(s) 65, 292
MroXI GAANNNNTTC 1 cut(s) 201
MseI TTAA 3 cut(s) 228, 465, 491
MslI CAYNNNNRTG 1 cut(s) 64
MspA1I CMGCKG 2 cut(s) 431, 570
MspR9I CCNGG 2 cut(s) 177, 379
Mva1269I GAATGC 1 cut(s) 97
MvaI CCWGG 2 cut(s) 177, 379
MwoI GCNNNNNNNGC 1 cut(s) 348
NarI GGCGCC 1 cut(s) 530
NdeII GATC 1 cut(s) 386
NlaIII CATG 3 cut(s) 69, 143, 394
NlaIV GGNNCC 3 cut(s) 376, 388, 531
NmuCI GTSAC 2 cut(s) 25, 393
PctI GAATGC 1 cut(s) 97
PdmI GAANNNNTTC 1 cut(s) 201
PfeI GAWTC 1 cut(s) 83
PkrI GCNGC 1 cut(s) 569
PluTI GGCGCC 1 cut(s) 533
PpuMI RGGWCCY 1 cut(s) 375
Psp5II RGGWCCY 1 cut(s) 375
Psp6I CCWGG 2 cut(s) 175, 377
PspGI CCWGG 2 cut(s) 175, 377
PspN4I GGNNCC 3 cut(s) 376, 388, 531
PspPI GGNCC 1 cut(s) 375
PspPPI RGGWCCY 1 cut(s) 375
PsuI RGATCY 1 cut(s) 386
PvuII CAGCTG 1 cut(s) 431
RsaI GTAC 1 cut(s) 615
RsaNI GTAC 1 cut(s) 614
RseI CAYNNNNRTG 1 cut(s) 64
SaqAI TTAA 3 cut(s) 228, 465, 491
SatI GCNGC 1 cut(s) 568
Sau3AI GATC 1 cut(s) 386
Sau96I GGNCC 1 cut(s) 375
ScaI AGTACT 1 cut(s) 615
ScrFI CCNGG 2 cut(s) 177, 379
SduI GDGCHC 2 cut(s) 344, 408
SfoI GGCGCC 1 cut(s) 531
SinI GGWCC 1 cut(s) 375
SmiMI CAYNNNNRTG 1 cut(s) 64
Sse9I AATT 2 cut(s) 448, 473
SsiI CCGC 1 cut(s) 570
SspDI GGCGCC 1 cut(s) 529
SspI AATATT 2 cut(s) 495, 600
StyD4I CCNGG 2 cut(s) 175, 377
StyI CCWWGG 2 cut(s) 369, 525
TaaI ACNGT 2 cut(s) 256, 613
TaiI ACGT 1 cut(s) 323
TasI AATT 2 cut(s) 448, 473
TatI WGTACW 1 cut(s) 613
TfiI GAWTC 1 cut(s) 83
Tru1I TTAA 3 cut(s) 228, 465, 491
Tru9I TTAA 3 cut(s) 228, 465, 491
TscAI CASTG 3 cut(s) 85, 261, 359
TseFI GTSAC 2 cut(s) 25, 393
TseI GCWGC 1 cut(s) 567
Tsp45I GTSAC 2 cut(s) 25, 393
TspDTI ATGAA 2 cut(s) 426, 451
TspRI CASTG 3 cut(s) 85, 261, 359
VpaK11BI GGWCC 1 cut(s) 375
XapI RAATTY 2 cut(s) 448, 473
XmnI GAANNNNTTC 1 cut(s) 201
ZrmI AGTACT 1 cut(s) 615
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.