Rh5DG418600

NEMP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0001753
Physical Location & Seq
Forward (+)
66574205 .. 66578189
3985 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG418600.1

Sequence Viewer

Length: 744 bp
ATGGCTTCTTCTAAGCAGGTTCAGGTCACAGCTTGTATTCTATGGCTTCTGTTCCTCACACTTCCATGTTCTTCTTCCACTGAATCACTCTATCAGCATTCTTTGGTTATTGGTGAAAATGCTACTTTAGGATTGTCTCATGGTATGCCAGTCGGAAAATCTCCCGGTTCTAAGCCTGGCGCTTCGGTGTTGTGTGAACGAGTTCTTATCTATGGATTGTCAAGGCTTAAACATCCGGATAAGTTTGGCAATACGGTGAAGGTGAAGATATCAACAACATCAAGATTTCAGACTTTTGAGGTTTGTTTCCATAGAAATACGTCCCGTGGGATAGGAATGTGCCCTAACAGCCAGTGGGAGAAAGTTTCCAAGGGGTCCTGGTCTGGATCCATGCCACCTTTTGAGCACAAACTCTTGGATATACGAACAGCTGCAAGGGAAATCTTTAGATGGATCCGTGGTCGTCACATGTACAGGAAATTGTTGAGATCACCCAAAAAGAAGAGCAGAAGATTAGAGATTCCCGATTCTTCACCTTCTGAATGTGCTCCTAATGAAGTCGTTAACGAGTTTCAGAGCAATGAGGACTATAAGCTACCGAGGCCTGGTTCCAGAAACTCTACCTCGGCTTCACGCATTTCACCTGGCCTAGGTTCTAAAAGTGCATCACCATCAGATGGAAGGTTATATCCTTCTTACATCCACACTGCACCTGAAAGGAGAGCAATGACAAAACATGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NEMP

Protein Analysis

247

Amino Acids

27.55

Weight (kDa)

9.99

Isoelectric Point (pI)

58.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 7
AccB7I CCANNNNNTGG 1 cut(s) 677
AccIII TCCGGA 1 cut(s) 235
AclWI GGATC 4 cut(s) 381, 394, 448, 461
AfaI GTAC 1 cut(s) 473
AfiI CCNNNNNNNGG 3 cut(s) 605, 650, 677
AflIII ACRYGT 1 cut(s) 468
AjnI CCWGG 4 cut(s) 175, 377, 604, 643
AluBI AGCT 3 cut(s) 32, 431, 595
AluI AGCT 3 cut(s) 32, 431, 595
Alw21I GWGCWC 2 cut(s) 408, 550
Alw26I GTCTC 1 cut(s) 141
AlwI GGATC 4 cut(s) 381, 394, 448, 461
Aor13HI TCCGGA 1 cut(s) 235
AoxI GGCC 2 cut(s) 602, 646
ApeKI GCWGC 1 cut(s) 431
Asp700I GAANNNNTTC 1 cut(s) 201
AspA2I CCTAGG 1 cut(s) 649
AspLEI GCGC 1 cut(s) 182
AspS9I GGNCC 1 cut(s) 375
AsuC2I CCSGG 1 cut(s) 165
AsuHPI GGTGA 7 cut(s) 125, 268, 274, 483, 525, 633, 660
AvaII GGWCC 1 cut(s) 375
AvrII CCTAGG 1 cut(s) 649
BaeGI GKGCMC 1 cut(s) 344
BamHI GGATCC 2 cut(s) 386, 453
Bbv12I GWGCWC 2 cut(s) 408, 550
BbvI GCAGC 1 cut(s) 418
BccI CCATC 3 cut(s) 444, 671, 679
BciT130I CCWGG 4 cut(s) 177, 379, 606, 645
BcnI CCSGG 1 cut(s) 165
BcoDI GTCTC 1 cut(s) 141
BfaI CTAG 1 cut(s) 650
BfoI RGCGCY 1 cut(s) 183
BfuAI ACCTGC 1 cut(s) 7
BisI GCNGC 1 cut(s) 432
BlnI CCTAGG 1 cut(s) 649
BlsI GCNGC 1 cut(s) 433
Bme1390I CCNGG 5 cut(s) 165, 177, 379, 606, 645
Bme18I GGWCC 1 cut(s) 375
BmgT120I GGNCC 1 cut(s) 375
BmiI GGNNCC 4 cut(s) 376, 388, 455, 610
BmrFI CCNGG 5 cut(s) 165, 177, 379, 606, 645
BmsI GCATC 1 cut(s) 674
BpuMI CCSGG 1 cut(s) 165
BsaJI CCNNGG 6 cut(s) 325, 369, 457, 599, 624, 649
BsaWI WCCGGW 1 cut(s) 235
Bsc4I CCNNNNNNNGG 3 cut(s) 605, 650, 677
Bse1I ACTGG 2 cut(s) 149, 352
Bse3DI GCAATG 2 cut(s) 586, 732
BseAI TCCGGA 1 cut(s) 235
BseBI CCWGG 4 cut(s) 177, 379, 606, 645
BseDI CCNNGG 6 cut(s) 325, 369, 457, 599, 624, 649
BseGI GGATG 2 cut(s) 232, 699
BseLI CCNNNNNNNGG 3 cut(s) 605, 650, 677
BseMI GCAATG 2 cut(s) 586, 732
BseNI ACTGG 2 cut(s) 149, 352
BseSI GKGCMC 1 cut(s) 344
BseXI GCAGC 1 cut(s) 418
BsgI GTGCAG 1 cut(s) 693
BshFI GGCC 2 cut(s) 604, 648
BsiHKAI GWGCWC 2 cut(s) 408, 550
BsiSI CCGG 2 cut(s) 165, 236
BslFI GGGAC 1 cut(s) 307
BslI CCNNNNNNNGG 3 cut(s) 605, 650, 677
BsmAI GTCTC 1 cut(s) 141
BsmFI GGGAC 1 cut(s) 307
BsmI GAATGC 1 cut(s) 97
BsnI GGCC 2 cut(s) 604, 648
Bsp1286I GDGCHC 3 cut(s) 344, 408, 550
Bsp13I TCCGGA 1 cut(s) 235
Bsp1407I TGTACA 1 cut(s) 471
Bsp143I GATC 3 cut(s) 386, 453, 488
BspANI GGCC 2 cut(s) 604, 648
BspEI TCCGGA 1 cut(s) 235
BspLI GGNNCC 4 cut(s) 376, 388, 455, 610
BspMI ACCTGC 1 cut(s) 7
BspPI GGATC 4 cut(s) 381, 394, 448, 461
BspQI GCTCTTC 1 cut(s) 497
BsrDI GCAATG 2 cut(s) 586, 732
BsrGI TGTACA 1 cut(s) 471
BsrI ACTGG 2 cut(s) 149, 352
BssECI CCNNGG 6 cut(s) 325, 369, 457, 599, 624, 649
BssMI GATC 3 cut(s) 386, 453, 488
BssT1I CCWWGG 2 cut(s) 369, 649
Bst2UI CCWGG 4 cut(s) 177, 379, 606, 645
Bst4CI ACNGT 1 cut(s) 256
Bst6I CTCTTC 1 cut(s) 497
BstAUI TGTACA 1 cut(s) 471
BstDEI CTNAG 2 cut(s) 12, 171
BstDSI CCRYGG 2 cut(s) 325, 457
BstENI CCTNNNNNAGG 1 cut(s) 648
BstF5I GGATG 2 cut(s) 232, 699
BstH2I RGCGCY 1 cut(s) 183
BstHHI GCGC 1 cut(s) 182
BstKTI GATC 3 cut(s) 389, 456, 491
BstMAI GTCTC 1 cut(s) 141
BstMBI GATC 3 cut(s) 386, 453, 488
BstMWI GCNNNNNNNGC 2 cut(s) 348, 601
BstNI CCWGG 4 cut(s) 177, 379, 606, 645
BstNSI RCATGY 1 cut(s) 472
BstSCI CCNGG 5 cut(s) 163, 175, 377, 604, 643
BstSLI GKGCMC 1 cut(s) 344
BstV1I GCAGC 1 cut(s) 418
BstX2I RGATCY 2 cut(s) 386, 453
BstYI RGATCY 2 cut(s) 386, 453
BsuRI GGCC 2 cut(s) 604, 648
BtgI CCRYGG 2 cut(s) 325, 457
BtsCI GGATG 2 cut(s) 232, 699
BtsI GCAGTG 1 cut(s) 705
BtsIMutI CAGTG 3 cut(s) 78, 359, 705
BveI ACCTGC 1 cut(s) 7
CfoI GCGC 1 cut(s) 182
Cfr13I GGNCC 1 cut(s) 375
Csp6I GTAC 1 cut(s) 472
CviAII CATG 5 cut(s) 66, 140, 391, 469, 737
CviQI GTAC 1 cut(s) 472
DdeI CTNAG 2 cut(s) 12, 171
DpnI GATC 3 cut(s) 388, 455, 490
DpnII GATC 3 cut(s) 386, 453, 488
Eam1104I CTCTTC 1 cut(s) 497
EarI CTCTTC 1 cut(s) 497
Eco130I CCWWGG 2 cut(s) 369, 649
Eco147I AGGCCT 1 cut(s) 604
Eco32I GATATC 1 cut(s) 270
Eco47I GGWCC 1 cut(s) 375
EcoNI CCTNNNNNAGG 1 cut(s) 648
EcoO109I RGGNCCY 1 cut(s) 375
EcoRII CCWGG 4 cut(s) 175, 377, 604, 643
EcoRV GATATC 1 cut(s) 270
EcoT14I CCWWGG 2 cut(s) 369, 649
ErhI CCWWGG 2 cut(s) 369, 649
FaeI CATG 5 cut(s) 69, 143, 394, 472, 740
FaqI GGGAC 1 cut(s) 307
FatI CATG 5 cut(s) 65, 139, 390, 468, 736
Fnu4HI GCNGC 1 cut(s) 432
FokI GGATG 2 cut(s) 219, 686
Fsp4HI GCNGC 1 cut(s) 432
FspBI CTAG 1 cut(s) 650
GlaI GCGC 1 cut(s) 181
GluI GCNGC 1 cut(s) 432
HaeII RGCGCY 1 cut(s) 183
HaeIII GGCC 2 cut(s) 604, 648
HapII CCGG 2 cut(s) 165, 236
HhaI GCGC 1 cut(s) 182
Hin1II CATG 5 cut(s) 69, 143, 394, 472, 740
Hin6I GCGC 1 cut(s) 180
HinP1I GCGC 1 cut(s) 180
HincII GTYRAC 1 cut(s) 565
HindII GTYRAC 1 cut(s) 565
HinfI GANTC 3 cut(s) 83, 520, 527
HpaI GTTAAC 1 cut(s) 565
HpaII CCGG 2 cut(s) 165, 236
HphI GGTGA 7 cut(s) 125, 268, 274, 483, 525, 633, 660
Hpy166II GTNNAC 2 cut(s) 197, 565
Hpy188I TCNGA 5 cut(s) 155, 291, 541, 576, 676
Hpy188III TCNNGA 5 cut(s) 236, 282, 384, 524, 612
Hpy8I GTNNAC 2 cut(s) 197, 565
HpyAV CCTTC 4 cut(s) 253, 546, 675, 702
HpyCH4III ACNGT 1 cut(s) 256
HpyCH4IV ACGT 1 cut(s) 320
HpyCH4V TGCA 3 cut(s) 434, 665, 710
HpyF10VI GCNNNNNNNGC 2 cut(s) 348, 601
HpyF3I CTNAG 2 cut(s) 12, 171
HpySE526I ACGT 1 cut(s) 320
Hsp92II CATG 5 cut(s) 69, 143, 394, 472, 740
HspAI GCGC 1 cut(s) 180
Kpn2I TCCGGA 1 cut(s) 235
KspAI GTTAAC 1 cut(s) 565
Kzo9I GATC 3 cut(s) 386, 453, 488
LguI GCTCTTC 1 cut(s) 497
LmnI GCTCC 1 cut(s) 553
Lsp1109I GCAGC 1 cut(s) 418
LweI GCATC 1 cut(s) 674
MaeI CTAG 1 cut(s) 650
MaeII ACGT 1 cut(s) 320
MaeIII GTNAC 2 cut(s) 25, 464
MalI GATC 3 cut(s) 388, 455, 490
MboI GATC 3 cut(s) 386, 453, 488
MboII GAAGA 6 cut(s) 63, 66, 277, 514, 522, 522
MflI RGATCY 2 cut(s) 386, 453
MhlI GDGCHC 3 cut(s) 344, 408, 550
MluCI AATT 1 cut(s) 479
MmeI TCCRAC 1 cut(s) 133
MnlI CCTC 5 cut(s) 65, 292, 577, 594, 634
MroI TCCGGA 1 cut(s) 235
MroXI GAANNNNTTC 1 cut(s) 201
MseI TTAA 2 cut(s) 228, 564
MslI CAYNNNNRTG 1 cut(s) 64
MspA1I CMGCKG 1 cut(s) 431
MspI CCGG 2 cut(s) 165, 236
MspR9I CCNGG 5 cut(s) 165, 177, 379, 606, 645
Mva1269I GAATGC 1 cut(s) 97
MvaI CCWGG 4 cut(s) 177, 379, 606, 645
MwoI GCNNNNNNNGC 2 cut(s) 348, 601
NciI CCSGG 1 cut(s) 165
NdeII GATC 3 cut(s) 386, 453, 488
NlaIII CATG 5 cut(s) 69, 143, 394, 472, 740
NlaIV GGNNCC 4 cut(s) 376, 388, 455, 610
NmeAIII GCCGAG 1 cut(s) 605
NmuCI GTSAC 2 cut(s) 25, 464
NspI RCATGY 1 cut(s) 472
PceI AGGCCT 1 cut(s) 604
PciI ACATGT 1 cut(s) 468
PciSI GCTCTTC 1 cut(s) 497
PctI GAATGC 1 cut(s) 97
PdmI GAANNNNTTC 1 cut(s) 201
PfeI GAWTC 3 cut(s) 83, 520, 527
PflMI CCANNNNNTGG 1 cut(s) 677
PkrI GCNGC 1 cut(s) 433
PpuMI RGGWCCY 1 cut(s) 375
PscI ACATGT 1 cut(s) 468
Psp5II RGGWCCY 1 cut(s) 375
Psp6I CCWGG 4 cut(s) 175, 377, 604, 643
PspGI CCWGG 4 cut(s) 175, 377, 604, 643
PspN4I GGNNCC 4 cut(s) 376, 388, 455, 610
PspPI GGNCC 1 cut(s) 375
PspPPI RGGWCCY 1 cut(s) 375
PsuI RGATCY 2 cut(s) 386, 453
PvuII CAGCTG 1 cut(s) 431
RsaI GTAC 1 cut(s) 473
RsaNI GTAC 1 cut(s) 472
RseI CAYNNNNRTG 1 cut(s) 64
SapI GCTCTTC 1 cut(s) 497
SaqAI TTAA 2 cut(s) 228, 564
SatI GCNGC 1 cut(s) 432
Sau3AI GATC 3 cut(s) 386, 453, 488
Sau96I GGNCC 1 cut(s) 375
ScrFI CCNGG 5 cut(s) 165, 177, 379, 606, 645
SduI GDGCHC 3 cut(s) 344, 408, 550
SfaNI GCATC 1 cut(s) 674
SinI GGWCC 1 cut(s) 375
SmiMI CAYNNNNRTG 1 cut(s) 64
Sse9I AATT 1 cut(s) 479
SseBI AGGCCT 1 cut(s) 604
SspMI CTAG 1 cut(s) 650
StuI AGGCCT 1 cut(s) 604
StyD4I CCNGG 5 cut(s) 163, 175, 377, 604, 643
StyI CCWWGG 2 cut(s) 369, 649
TaaI ACNGT 1 cut(s) 256
TaiI ACGT 1 cut(s) 323
TasI AATT 1 cut(s) 479
TatI WGTACW 1 cut(s) 471
TfiI GAWTC 3 cut(s) 83, 520, 527
Tru1I TTAA 2 cut(s) 228, 564
Tru9I TTAA 2 cut(s) 228, 564
TscAI CASTG 3 cut(s) 85, 359, 712
TseFI GTSAC 2 cut(s) 25, 464
TseI GCWGC 1 cut(s) 431
Tsp45I GTSAC 2 cut(s) 25, 464
TspDTI ATGAA 1 cut(s) 570
TspGWI ACGGA 1 cut(s) 446
TspRI CASTG 3 cut(s) 85, 359, 712
Van91I CCANNNNNTGG 1 cut(s) 677
VpaK11BI GGWCC 1 cut(s) 375
XagI CCTNNNNNAGG 1 cut(s) 648
XceI RCATGY 1 cut(s) 472
XmaJI CCTAGG 1 cut(s) 649
XmnI GAANNNNTTC 1 cut(s) 201
XspI CTAG 1 cut(s) 650
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.