FvH4_1g17850

NEMP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0001753
Physical Location & Seq
Reverse (-)
10374351 .. 10378281
3931 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g17850.t2

Sequence Viewer

Length: 1578 bp
ATGGCTTCTTCTAAGCCGGTTCGGTTCGCTGCTTGCATTCTATGCCTTCTCTTCCTCACTCTCCCCTCTTCTTCTTCCTCTGAATCGCTGATTGACCATTCTCTAGTTGTTGGAGAGAATGCTACTTTGCAAGTCTCGAATGGTGTGGCGGTGCGGAATTCTCCCGGTGCTAAGCCTGGCAATTTAGTGATGTGTGGAAGAGTACATGTCTTTGGATTGTCAAGGCTTAAGAATTTGGAGAGGTTTGCCGACACGGTGAGGGTGAAGATAGAGATAAATTCGAGCTATCGTGTGCGGCCTTTTGAGGTTTGCTTCCATAGAAATACATCTCGCGGGATAGGAATGTGCCCTCACAGTCAGTGGGAAAAAGTTTCCAAGGGGTCCTGGTCTGGATCCATGTCACCTTATGAGCACAAGATCGTTGATATACGAACAGCTGCTTCATCCTTGGATATTTTTGAGGTGTCCCTTGAAGAAGAATTTTCTATATCTCGCCTGGTATTTTTTGTATCTGGTGTAATCTTGATGAGTTTGGCACCTTCTCTGAGCAATTCACTGGTATTCTATTACGGCAGTGGAATGGCAATAGGGGTGGTCCTTGTAATATTGATTGTCCTTTTTCAGGGGATGAAGCTTCTCCCAACAGGTCGGAAGAATTCGCTCGGAATTTTTTTGTTTTCATCTGTGATTGGTTTAGGATCTTTTCTCCTCAGCTACATCCCGGGGCTAATCCGATCAGTACTCACAGAGCTTGGAATCAGCGAAGAGATGTATAATCCTTTGGTTATATTTCTTCTGGCTTTTATTTTTCTGGCTGGAGCATGGTTGGGATTCTGGACAGTTCGTAAAATGGCCCTGAGAGAAGATGGATCAATTGATTCTAGCACATCTCTGTTTGTTTTGTGGACAATCCGGTTCGTAGCCGTTCTTATGCTTTCACAGAGTTCTAGTGATCAGCTACTGGCAGCAGGAGCCATAACAGTCCCTTTAGTCCCTTTTATAGTGATGAAAGTTGCTATGGTGTTTGGAGTTGTACTCTCTTTTATGAGGGAAATAACTATAGCATTTGGAACAATAGTCTCTTTTATAGCAAGGACCATTGCTATAATAGTTGGAGGCGTATTCTCTTCAGTGCGGGAAATCTTCAGATGGATTCGTCACCGTCGCATGTACAGGAAATTGTTGAAATCACCCAAAAAGAAGAGCAGAAGATCAGAGATTCCTGATTCTCCATCTTCTGAATATGCACCTGATGAGATCATCAACGAGTTTCAAAGCGATGAAGACTATAAGTTCCCGAGGCCTGGATCGAGAAATTCTACGCTGGCTTCATGTTCTAGAAGTGCATCACCATCAGAAAGGTTATACCCTTCTTATATCCACACTACACCCAAGAGGAGAGCAGTGTCAAGACATGAGTGGGAAAGTTTCACCAGACAGACAACGGAGAAAGCCTTGGAAGAACTTGCTTCTTCTCCTGATTTCGGCAAGTGGGTAGTTGCAAATGCTGATAGAATCAGCATCACCCCACTCAGCAGCAGAAAGACTGATGAGCAGCGCCACAGGTGGTGGTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NEMP

Protein Analysis

526

Amino Acids

58.62

Weight (kDa)

9.71

Isoelectric Point (pI)

54.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NEMP PF10225 154 - 337 3e-33 NEMP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 535
AccII CGCG 1 cut(s) 333
AciI CCGC 5 cut(s) 149, 154, 295, 333, 1135
AclWI GGATC 5 cut(s) 387, 400, 706, 877, 1315
AcsI RAATTY 7 cut(s) 157, 232, 277, 479, 655, 666, 1315
AcuI CTGAAG 2 cut(s) 1113, 1129
AfaI GTAC 4 cut(s) 204, 741, 1035, 1172
AfiI CCNNNNNNNGG 3 cut(s) 622, 1304, 1484
AflII CTTAAG 1 cut(s) 227
AflIII ACRYGT 1 cut(s) 205
AgsI TTSAA 3 cut(s) 473, 1186, 1274
AjnI CCWGG 4 cut(s) 175, 383, 495, 1303
AluBI AGCT 6 cut(s) 285, 437, 634, 714, 751, 958
AluI AGCT 6 cut(s) 285, 437, 634, 714, 751, 958
Alw21I GWGCWC 1 cut(s) 414
Alw26I GTCTC 2 cut(s) 139, 1084
AlwI GGATC 5 cut(s) 387, 400, 706, 877, 1315
AlwNI CAGNNNCTG 1 cut(s) 961
Ama87I CYCGRG 2 cut(s) 721, 1297
AoxI GGCC 3 cut(s) 296, 852, 1301
ApeKI GCWGC 5 cut(s) 29, 437, 965, 1536, 1555
ApoI RAATTY 7 cut(s) 157, 232, 277, 479, 655, 666, 1315
AspLEI GCGC 1 cut(s) 1560
AspS9I GGNCC 4 cut(s) 381, 595, 853, 1095
AsuC2I CCSGG 3 cut(s) 165, 722, 723
AsuHPI GGTGA 8 cut(s) 268, 274, 393, 1151, 1182, 1341, 1423, 1516
AvaI CYCGRG 2 cut(s) 721, 1297
AvaII GGWCC 3 cut(s) 381, 595, 1095
BaeGI GKGCMC 1 cut(s) 350
BamHI GGATCC 1 cut(s) 392
BanI GGYRCC 1 cut(s) 535
BbsI GAAGAC 1 cut(s) 1290
Bbv12I GWGCWC 1 cut(s) 414
BbvCI CCTCAGC 1 cut(s) 710
BbvI GCAGC 5 cut(s) 16, 424, 977, 1548, 1567
BccI CCATC 4 cut(s) 860, 1143, 1240, 1360
BceAI ACGGC 2 cut(s) 586, 908
BciT130I CCWGG 4 cut(s) 177, 385, 497, 1305
BclI TGATCA 1 cut(s) 952
BcnI CCSGG 3 cut(s) 165, 722, 723
BcoDI GTCTC 2 cut(s) 139, 1084
BfaI CTAG 5 cut(s) 104, 882, 948, 1338, 1576
BfmI CTRYAG 1 cut(s) 1059
BfoI RGCGCY 1 cut(s) 1561
BfrI CTTAAG 1 cut(s) 227
BisI GCNGC 6 cut(s) 30, 296, 438, 966, 1537, 1556
BlpI GCTNAGC 1 cut(s) 171
BlsI GCNGC 6 cut(s) 31, 297, 439, 967, 1538, 1557
BmcAI AGTACT 1 cut(s) 741
Bme1390I CCNGG 7 cut(s) 165, 177, 385, 497, 722, 723, 1305
Bme18I GGWCC 3 cut(s) 381, 595, 1095
BmeT110I CYCGRG 2 cut(s) 721, 1297
BmgT120I GGNCC 4 cut(s) 381, 595, 853, 1095
BmiI GGNNCC 4 cut(s) 382, 394, 537, 973
BmrFI CCNGG 7 cut(s) 165, 177, 385, 497, 722, 723, 1305
BmsI GCATC 2 cut(s) 1355, 1530
BpiI GAAGAC 1 cut(s) 1290
BplI GAGNNNNNCTC 2 cut(s) 1020, 1052
BpmI CTGGAG 1 cut(s) 837
Bpu10I CCTNAGC 1 cut(s) 710
Bpu1102I GCTNAGC 1 cut(s) 171
BpuMI CCSGG 3 cut(s) 165, 722, 723
BsaJI CCNNGG 6 cut(s) 375, 447, 721, 722, 1298, 1455
BsaWI WCCGGW 1 cut(s) 912
Bsc4I CCNNNNNNNGG 3 cut(s) 622, 1304, 1484
Bse118I RCCGGY 1 cut(s) 16
Bse1I ACTGG 2 cut(s) 561, 966
Bse3DI GCAATG 1 cut(s) 1098
BseBI CCWGG 4 cut(s) 177, 385, 497, 1305
BseDI CCNNGG 6 cut(s) 375, 447, 721, 722, 1298, 1455
BseGI GGATG 3 cut(s) 443, 633, 717
BseLI CCNNNNNNNGG 3 cut(s) 622, 1304, 1484
BseMI GCAATG 1 cut(s) 1098
BseMII CTCAG 4 cut(s) 536, 724, 848, 1546
BseNI ACTGG 2 cut(s) 561, 966
BseRI GAGGAG 2 cut(s) 698, 1411
BseSI GKGCMC 1 cut(s) 350
BseXI GCAGC 5 cut(s) 16, 424, 977, 1548, 1567
Bsh1236I CGCG 1 cut(s) 333
BshFI GGCC 3 cut(s) 298, 854, 1303
BshNI GGYRCC 1 cut(s) 535
BsiHKAI GWGCWC 1 cut(s) 414
BsiHKCI CYCGRG 2 cut(s) 721, 1297
BsiSI CCGG 4 cut(s) 17, 165, 722, 913
BslFI GGGAC 3 cut(s) 451, 968, 977
BslI CCNNNNNNNGG 3 cut(s) 622, 1304, 1484
BsmAI GTCTC 2 cut(s) 139, 1084
BsmFI GGGAC 3 cut(s) 451, 968, 977
BsmI GAATGC 2 cut(s) 36, 124
BsnI GGCC 3 cut(s) 298, 854, 1303
BsoBI CYCGRG 2 cut(s) 721, 1297
Bsp1286I GDGCHC 2 cut(s) 350, 414
Bsp1407I TGTACA 1 cut(s) 1170
Bsp143I GATC 9 cut(s) 392, 417, 698, 734, 869, 952, 1211, 1257, 1307
Bsp1720I GCTNAGC 1 cut(s) 171
BspACI CCGC 5 cut(s) 149, 154, 295, 333, 1135
BspANI GGCC 3 cut(s) 298, 854, 1303
BspCNI CTCAG 4 cut(s) 537, 723, 849, 1545
BspFNI CGCG 1 cut(s) 333
BspLI GGNNCC 4 cut(s) 382, 394, 537, 973
BspPI GGATC 5 cut(s) 387, 400, 706, 877, 1315
BspQI GCTCTTC 1 cut(s) 1196
BspT107I GGYRCC 1 cut(s) 535
BspTI CTTAAG 1 cut(s) 227
BsrDI GCAATG 1 cut(s) 1098
BsrFI RCCGGY 1 cut(s) 16
BsrGI TGTACA 1 cut(s) 1170
BsrI ACTGG 2 cut(s) 561, 966
BssAI RCCGGY 1 cut(s) 16
BssECI CCNNGG 6 cut(s) 375, 447, 721, 722, 1298, 1455
BssMI GATC 9 cut(s) 392, 417, 698, 734, 869, 952, 1211, 1257, 1307
BssT1I CCWWGG 3 cut(s) 375, 447, 1455
Bst2UI CCWGG 4 cut(s) 177, 385, 497, 1305
Bst4CI ACNGT 5 cut(s) 256, 356, 841, 982, 1163
Bst6I CTCTTC 6 cut(s) 56, 73, 193, 759, 1132, 1196
BstAFI CTTAAG 1 cut(s) 227
BstAPI GCANNNNNTGC 1 cut(s) 42
BstAUI TGTACA 1 cut(s) 1170
BstC8I GCNNGC 2 cut(s) 34, 1326
BstDEI CTNAG 6 cut(s) 12, 171, 545, 710, 857, 1532
BstENI CCTNNNNNAGG 1 cut(s) 620
BstF5I GGATG 3 cut(s) 443, 633, 717
BstFNI CGCG 1 cut(s) 333
BstH2I RGCGCY 1 cut(s) 1561
BstHHI GCGC 1 cut(s) 1560
BstKTI GATC 9 cut(s) 395, 420, 701, 737, 872, 955, 1214, 1260, 1310
BstMAI GTCTC 2 cut(s) 139, 1084
BstMBI GATC 9 cut(s) 392, 417, 698, 734, 869, 952, 1211, 1257, 1307
BstMWI GCNNNNNNNGC 2 cut(s) 42, 971
BstNI CCWGG 4 cut(s) 177, 385, 497, 1305
BstNSI RCATGY 2 cut(s) 209, 1171
BstSCI CCNGG 7 cut(s) 163, 175, 383, 495, 720, 721, 1303
BstSFI CTRYAG 1 cut(s) 1059
BstSLI GKGCMC 1 cut(s) 350
BstUI CGCG 1 cut(s) 333
BstV1I GCAGC 5 cut(s) 16, 424, 977, 1548, 1567
BstV2I GAAGAC 1 cut(s) 1290
BstX2I RGATCY 2 cut(s) 392, 698
BstYI RGATCY 2 cut(s) 392, 698
BsuRI GGCC 3 cut(s) 298, 854, 1303
BtgZI GCGATG 1 cut(s) 1293
BtsCI GGATG 3 cut(s) 443, 633, 717
BtsI GCAGTG 2 cut(s) 580, 1410
BtsIMutI CAGTG 5 cut(s) 365, 554, 580, 1137, 1410
Cac8I GCNNGC 2 cut(s) 34, 1326
CaiI CAGNNNCTG 1 cut(s) 961
CfoI GCGC 1 cut(s) 1560
Cfr10I RCCGGY 1 cut(s) 16
Cfr13I GGNCC 4 cut(s) 381, 595, 853, 1095
Cfr9I CCCGGG 1 cut(s) 721
Csp6I GTAC 4 cut(s) 203, 740, 1034, 1171
CspCI CAANNNNNGTGG 2 cut(s) 573, 608
CviAII CATG 6 cut(s) 206, 397, 822, 1168, 1332, 1415
CviQI GTAC 4 cut(s) 203, 740, 1034, 1171
DdeI CTNAG 6 cut(s) 12, 171, 545, 710, 857, 1532
DpnI GATC 9 cut(s) 394, 419, 700, 736, 871, 954, 1213, 1259, 1309
DpnII GATC 9 cut(s) 392, 417, 698, 734, 869, 952, 1211, 1257, 1307
Eam1104I CTCTTC 6 cut(s) 56, 73, 193, 759, 1132, 1196
EarI CTCTTC 6 cut(s) 56, 73, 193, 759, 1132, 1196
Eco130I CCWWGG 3 cut(s) 375, 447, 1455
Eco147I AGGCCT 1 cut(s) 1303
Eco47I GGWCC 3 cut(s) 381, 595, 1095
Eco57I CTGAAG 2 cut(s) 1113, 1129
Eco88I CYCGRG 2 cut(s) 721, 1297
EcoNI CCTNNNNNAGG 1 cut(s) 620
EcoO109I RGGNCCY 1 cut(s) 381
EcoRI GAATTC 2 cut(s) 157, 655
EcoRII CCWGG 4 cut(s) 175, 383, 495, 1303
EcoT14I CCWWGG 3 cut(s) 375, 447, 1455
ErhI CCWWGG 3 cut(s) 375, 447, 1455
FaeI CATG 6 cut(s) 209, 400, 825, 1171, 1335, 1418
FaqI GGGAC 3 cut(s) 451, 968, 977
FatI CATG 6 cut(s) 205, 396, 821, 1167, 1331, 1414
FauI CCCGC 2 cut(s) 326, 1128
FbaI TGATCA 1 cut(s) 952
Fnu4HI GCNGC 6 cut(s) 30, 296, 438, 966, 1537, 1556
FokI GGATG 3 cut(s) 430, 640, 704
Fsp4HI GCNGC 6 cut(s) 30, 296, 438, 966, 1537, 1556
FspBI CTAG 5 cut(s) 104, 882, 948, 1338, 1576
GlaI GCGC 1 cut(s) 1559
GluI GCNGC 6 cut(s) 30, 296, 438, 966, 1537, 1556
GsuI CTGGAG 1 cut(s) 837
HaeII RGCGCY 1 cut(s) 1561
HaeIII GGCC 3 cut(s) 298, 854, 1303
HapII CCGG 4 cut(s) 17, 165, 722, 913
HhaI GCGC 1 cut(s) 1560
Hin1II CATG 6 cut(s) 209, 400, 825, 1171, 1335, 1418
Hin6I GCGC 1 cut(s) 1558
HinP1I GCGC 1 cut(s) 1558
HindIII AAGCTT 1 cut(s) 632
HinfI GANTC 8 cut(s) 83, 756, 831, 878, 1153, 1219, 1226, 1515
HpaII CCGG 4 cut(s) 17, 165, 722, 913
HphI GGTGA 8 cut(s) 268, 274, 393, 1151, 1182, 1341, 1423, 1516
Hpy166II GTNNAC 1 cut(s) 906
Hpy188I TCNGA 9 cut(s) 82, 546, 651, 665, 734, 1148, 1216, 1240, 1357
Hpy8I GTNNAC 1 cut(s) 906
Hpy99I CGWCG 1 cut(s) 1167
HpyAV CCTTC 3 cut(s) 56, 549, 1380
HpyCH4III ACNGT 5 cut(s) 256, 356, 841, 982, 1163
HpyCH4V TGCA 5 cut(s) 36, 130, 1247, 1346, 1502
HpyF10VI GCNNNNNNNGC 2 cut(s) 42, 971
HpyF3I CTNAG 6 cut(s) 12, 171, 545, 710, 857, 1532
Hsp92II CATG 6 cut(s) 209, 400, 825, 1171, 1335, 1418
HspAI GCGC 1 cut(s) 1558
Ksp22I TGATCA 1 cut(s) 952
Kzo9I GATC 9 cut(s) 392, 417, 698, 734, 869, 952, 1211, 1257, 1307
LguI GCTCTTC 1 cut(s) 1196
LmnI GCTCC 2 cut(s) 818, 971
Lsp1109I GCAGC 5 cut(s) 16, 424, 977, 1548, 1567
LweI GCATC 2 cut(s) 1355, 1530
MaeI CTAG 5 cut(s) 104, 882, 948, 1338, 1576
MaeIII GTNAC 2 cut(s) 399, 1157
MalI GATC 9 cut(s) 394, 419, 700, 736, 871, 954, 1213, 1259, 1309
MboI GATC 9 cut(s) 392, 417, 698, 734, 869, 952, 1211, 1257, 1307
MfeI CAATTG 1 cut(s) 873
MflI RGATCY 2 cut(s) 392, 698
MhlI GDGCHC 2 cut(s) 350, 414
MmeI TCCRAC 3 cut(s) 91, 629, 1093
MseI TTAA 1 cut(s) 228
MspA1I CMGCKG 1 cut(s) 437
MspCI CTTAAG 1 cut(s) 227
MspI CCGG 4 cut(s) 17, 165, 722, 913
MspR9I CCNGG 7 cut(s) 165, 177, 385, 497, 722, 723, 1305
MunI CAATTG 1 cut(s) 873
Mva1269I GAATGC 2 cut(s) 36, 124
MvaI CCWGG 4 cut(s) 177, 385, 497, 1305
MvnI CGCG 1 cut(s) 333
MwoI GCNNNNNNNGC 2 cut(s) 42, 971
NciI CCSGG 3 cut(s) 165, 722, 723
NdeII GATC 9 cut(s) 392, 417, 698, 734, 869, 952, 1211, 1257, 1307
NlaIII CATG 6 cut(s) 209, 400, 825, 1171, 1335, 1418
NlaIV GGNNCC 4 cut(s) 382, 394, 537, 973
NmuCI GTSAC 2 cut(s) 399, 1157
NspI RCATGY 2 cut(s) 209, 1171
PceI AGGCCT 1 cut(s) 1303
PciI ACATGT 1 cut(s) 205
PciSI GCTCTTC 1 cut(s) 1196
PctI GAATGC 2 cut(s) 36, 124
PfeI GAWTC 8 cut(s) 83, 756, 831, 878, 1153, 1219, 1226, 1515
PkrI GCNGC 6 cut(s) 31, 297, 439, 967, 1538, 1557
PpuMI RGGWCCY 1 cut(s) 381
PscI ACATGT 1 cut(s) 205
Psp5II RGGWCCY 1 cut(s) 381
Psp6I CCWGG 4 cut(s) 175, 383, 495, 1303
PspGI CCWGG 4 cut(s) 175, 383, 495, 1303
PspN4I GGNNCC 4 cut(s) 382, 394, 537, 973
PspPI GGNCC 4 cut(s) 381, 595, 853, 1095
PspPPI RGGWCCY 1 cut(s) 381
PstNI CAGNNNCTG 1 cut(s) 961
PsuI RGATCY 2 cut(s) 392, 698
PvuII CAGCTG 1 cut(s) 437
RsaI GTAC 4 cut(s) 204, 741, 1035, 1172
RsaNI GTAC 4 cut(s) 203, 740, 1034, 1171
SapI GCTCTTC 1 cut(s) 1196
SaqAI TTAA 1 cut(s) 228
SatI GCNGC 6 cut(s) 30, 296, 438, 966, 1537, 1556
Sau3AI GATC 9 cut(s) 392, 417, 698, 734, 869, 952, 1211, 1257, 1307
Sau96I GGNCC 4 cut(s) 381, 595, 853, 1095
ScaI AGTACT 1 cut(s) 741
ScrFI CCNGG 7 cut(s) 165, 177, 385, 497, 722, 723, 1305
SduI GDGCHC 2 cut(s) 350, 414
SfaNI GCATC 2 cut(s) 1355, 1530
SfcI CTRYAG 1 cut(s) 1059
SinI GGWCC 3 cut(s) 381, 595, 1095
SmaI CCCGGG 1 cut(s) 723
SmlI CTYRAG 1 cut(s) 227
SmoI CTYRAG 1 cut(s) 227
SseBI AGGCCT 1 cut(s) 1303
SsiI CCGC 5 cut(s) 149, 154, 295, 333, 1135
SspI AATATT 1 cut(s) 606
SspMI CTAG 5 cut(s) 104, 882, 948, 1338, 1576
StuI AGGCCT 1 cut(s) 1303
StyD4I CCNGG 7 cut(s) 163, 175, 383, 495, 720, 721, 1303
StyI CCWWGG 3 cut(s) 375, 447, 1455
TaaI ACNGT 5 cut(s) 256, 356, 841, 982, 1163
TaqI TCGA 3 cut(s) 137, 281, 1310
TatI WGTACW 4 cut(s) 202, 739, 1033, 1170
TauI GCSGC 1 cut(s) 298
TfiI GAWTC 8 cut(s) 83, 756, 831, 878, 1153, 1219, 1226, 1515
Tru1I TTAA 1 cut(s) 228
Tru9I TTAA 1 cut(s) 228
TscAI CASTG 5 cut(s) 365, 561, 580, 1137, 1410
TseFI GTSAC 2 cut(s) 399, 1157
TseI GCWGC 5 cut(s) 29, 437, 965, 1536, 1555
Tsp45I GTSAC 2 cut(s) 399, 1157
TspDTI ATGAA 6 cut(s) 432, 644, 669, 1022, 1296, 1320
TspGWI ACGGA 1 cut(s) 1460
TspMI CCCGGG 1 cut(s) 721
TspRI CASTG 5 cut(s) 365, 561, 580, 1137, 1410
Vha464I CTTAAG 1 cut(s) 227
VpaK11BI GGWCC 3 cut(s) 381, 595, 1095
XagI CCTNNNNNAGG 1 cut(s) 620
XapI RAATTY 7 cut(s) 157, 232, 277, 479, 655, 666, 1315
XbaI TCTAGA 1 cut(s) 1337
XceI RCATGY 2 cut(s) 209, 1171
XmaI CCCGGG 1 cut(s) 721
XspI CTAG 5 cut(s) 104, 882, 948, 1338, 1576
ZrmI AGTACT 1 cut(s) 741
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.