Rroxscaffold_1G00020760

NEMP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0001753
Physical Location & Seq
Reverse (-)
25252858 .. 25254125
1268 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00020760.1

Sequence Viewer

Length: 972 bp
ATGGCTTCTTCTAAGCAGGTTCAGGTCACAGCTTGTATTCTATGGCTTCTGTTCCTCACACTTCCATGTTCTTCTTCCACTGAATCACTCTATCAGCATTCTTTGGCTGTCGGTGAAAATGCTACTTTAGGATTGTCTCATGGTATGCCAGTCGGAAAATCTCCCGGTTCTAAGCCTGGCGCTTCGGTGTTGTGTGAACGAGTTCTTATCTATGGATTGTCAAGGCTTAAACATCTGGATAAGTTTGCCGATACAGTGAAGGTGAAGATATCAACAACATCAAGGTTTCAGACTTTTGAGGTAGTGAAGCTTCTCCCAACTGGTCGGAAGAATTCACTTGCAATTTTCGTCTTGTCATCTGTGGTTGGTTTGGGATCTTTTCTCCTCAGCTACATCCCAGGACTACTAACTTCAGTACTCACAGAGCTAGGAATTAGTGAGGATATGTATAATGCTTTGGCCATATTTCTTCTGGCTTTTGTTTTTCTGGCTGGAGCATGGTTGGGATTCTGGACAGTTCGTAAAATGGCCTTGAACGAAGATAGATCAATTGATTCTAGCACATCTCTGTTTGTTTTGTGGACCATTCGCTTCGTAGCGGTTATTTTTCTTTCACAGAGTTCCAGCGATCAACTACTGGCAGCAGGAGCTATAATGGTACCTTTAGTCACTTTTATAGTGAGGAAAATTGCCATTATACTAGGAACTTTAGTCCCTTTAATCAGGAAAATCACTATATCTTTTGGAACTATAGTCGTTTTCATAGCGAGGACATTCACTATAGTATGTGGATGCATAATCTCTTCAGCAAGGGAAATCTTTAGATGGATCCGTGGTCGTCACATGTACAGGAAATTGTTGAGATCACCCAAAAAGAAGAGCAGAAGAGTAGAGATTCCTGATTCTTCACCTTCTGAATGTGCTCCTAATGAATTCGTTAACGAGTTTCAGAGCAATGAGGACTATAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NEMP

Protein Analysis

323

Amino Acids

35.64

Weight (kDa)

9.67

Isoelectric Point (pI)

44.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NEMP PF10225 98 - 229 1e-11 NEMP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 7
Acc65I GGTACC 1 cut(s) 658
AccB1I GGYRCC 1 cut(s) 658
AciI CCGC 1 cut(s) 599
AclWI GGATC 3 cut(s) 382, 823, 836
AcoI YGGCCR 1 cut(s) 459
AcsI RAATTY 2 cut(s) 331, 932
AcuI CTGAAG 2 cut(s) 396, 789
AfaI GTAC 3 cut(s) 417, 660, 848
AflIII ACRYGT 1 cut(s) 843
AgsI TTSAA 1 cut(s) 535
AjnI CCWGG 2 cut(s) 175, 397
AluBI AGCT 5 cut(s) 32, 310, 390, 427, 650
AluI AGCT 5 cut(s) 32, 310, 390, 427, 650
Alw21I GWGCWC 1 cut(s) 925
Alw26I GTCTC 1 cut(s) 141
AlwI GGATC 3 cut(s) 382, 823, 836
AoxI GGCC 2 cut(s) 459, 528
ApeKI GCWGC 1 cut(s) 641
ApoI RAATTY 2 cut(s) 331, 932
Asp700I GAANNNNTTC 1 cut(s) 201
Asp718I GGTACC 1 cut(s) 658
AspLEI GCGC 1 cut(s) 182
AspS9I GGNCC 1 cut(s) 582
AsuC2I CCSGG 1 cut(s) 165
AsuHPI GGTGA 4 cut(s) 125, 274, 858, 900
AvaII GGWCC 1 cut(s) 582
BalI TGGCCA 1 cut(s) 461
BamHI GGATCC 1 cut(s) 828
BanI GGYRCC 1 cut(s) 658
BarI GAAGNNNNNNTAC 2 cut(s) 294, 326
Bbv12I GWGCWC 1 cut(s) 925
BbvCI CCTCAGC 1 cut(s) 386
BbvI GCAGC 1 cut(s) 653
BccI CCATC 1 cut(s) 819
BciT130I CCWGG 2 cut(s) 177, 399
BcnI CCSGG 1 cut(s) 165
BcoDI GTCTC 1 cut(s) 141
BfaI CTAG 3 cut(s) 428, 558, 701
BfmI CTRYAG 2 cut(s) 750, 780
BfoI RGCGCY 1 cut(s) 183
BfuAI ACCTGC 1 cut(s) 7
BisI GCNGC 1 cut(s) 642
BlsI GCNGC 1 cut(s) 643
BmcAI AGTACT 1 cut(s) 417
Bme1390I CCNGG 3 cut(s) 165, 177, 399
Bme18I GGWCC 1 cut(s) 582
BmgT120I GGNCC 1 cut(s) 582
BmiI GGNNCC 2 cut(s) 660, 830
BmrFI CCNGG 3 cut(s) 165, 177, 399
BmsI GCATC 1 cut(s) 782
BpmI CTGGAG 1 cut(s) 513
Bpu10I CCTNAGC 1 cut(s) 386
BpuMI CCSGG 1 cut(s) 165
BsaJI CCNNGG 2 cut(s) 397, 832
Bse1I ACTGG 3 cut(s) 149, 325, 642
Bse3DI GCAATG 1 cut(s) 961
BseBI CCWGG 2 cut(s) 177, 399
BseDI CCNNGG 2 cut(s) 397, 832
BseGI GGATG 2 cut(s) 393, 797
BseMI GCAATG 1 cut(s) 961
BseMII CTCAG 1 cut(s) 400
BseNI ACTGG 3 cut(s) 149, 325, 642
BseRI GAGGAG 1 cut(s) 374
BseXI GCAGC 1 cut(s) 653
BshFI GGCC 2 cut(s) 461, 530
BshNI GGYRCC 1 cut(s) 658
BsiHKAI GWGCWC 1 cut(s) 925
BsiSI CCGG 1 cut(s) 165
BslFI GGGAC 1 cut(s) 698
BsmAI GTCTC 1 cut(s) 141
BsmFI GGGAC 1 cut(s) 698
BsmI GAATGC 1 cut(s) 97
BsnI GGCC 2 cut(s) 461, 530
Bsp1286I GDGCHC 1 cut(s) 925
Bsp1407I TGTACA 1 cut(s) 846
Bsp143I GATC 5 cut(s) 374, 545, 628, 828, 863
BspACI CCGC 1 cut(s) 599
BspANI GGCC 2 cut(s) 461, 530
BspCNI CTCAG 1 cut(s) 399
BspLI GGNNCC 2 cut(s) 660, 830
BspMI ACCTGC 1 cut(s) 7
BspPI GGATC 3 cut(s) 382, 823, 836
BspQI GCTCTTC 1 cut(s) 872
BspT107I GGYRCC 1 cut(s) 658
BsrDI GCAATG 1 cut(s) 961
BsrGI TGTACA 1 cut(s) 846
BsrI ACTGG 3 cut(s) 149, 325, 642
BssECI CCNNGG 2 cut(s) 397, 832
BssMI GATC 5 cut(s) 374, 545, 628, 828, 863
Bst2UI CCWGG 2 cut(s) 177, 399
Bst4CI ACNGT 2 cut(s) 256, 517
Bst6I CTCTTC 3 cut(s) 808, 872, 880
BstAUI TGTACA 1 cut(s) 846
BstDEI CTNAG 3 cut(s) 12, 171, 386
BstDSI CCRYGG 1 cut(s) 832
BstF5I GGATG 2 cut(s) 393, 797
BstH2I RGCGCY 1 cut(s) 183
BstHHI GCGC 1 cut(s) 182
BstKTI GATC 5 cut(s) 377, 548, 631, 831, 866
BstMAI GTCTC 1 cut(s) 141
BstMBI GATC 5 cut(s) 374, 545, 628, 828, 863
BstMWI GCNNNNNNNGC 1 cut(s) 647
BstNI CCWGG 2 cut(s) 177, 399
BstNSI RCATGY 1 cut(s) 847
BstSCI CCNGG 3 cut(s) 163, 175, 397
BstSFI CTRYAG 2 cut(s) 750, 780
BstV1I GCAGC 1 cut(s) 653
BstX2I RGATCY 2 cut(s) 374, 828
BstYI RGATCY 2 cut(s) 374, 828
BsuRI GGCC 2 cut(s) 461, 530
BtgI CCRYGG 1 cut(s) 832
BtsCI GGATG 2 cut(s) 393, 797
BtsIMutI CAGTG 2 cut(s) 78, 261
BveI ACCTGC 1 cut(s) 7
CfoI GCGC 1 cut(s) 182
Cfr13I GGNCC 1 cut(s) 582
Csp6I GTAC 3 cut(s) 416, 659, 847
CviAII CATG 4 cut(s) 66, 140, 498, 844
CviQI GTAC 3 cut(s) 416, 659, 847
DdeI CTNAG 3 cut(s) 12, 171, 386
DpnI GATC 5 cut(s) 376, 547, 630, 830, 865
DpnII GATC 5 cut(s) 374, 545, 628, 828, 863
EaeI YGGCCR 1 cut(s) 459
Eam1104I CTCTTC 3 cut(s) 808, 872, 880
EarI CTCTTC 3 cut(s) 808, 872, 880
Eco32I GATATC 1 cut(s) 270
Eco47I GGWCC 1 cut(s) 582
Eco57I CTGAAG 2 cut(s) 396, 789
EcoRI GAATTC 2 cut(s) 331, 932
EcoRII CCWGG 2 cut(s) 175, 397
EcoRV GATATC 1 cut(s) 270
EcoT22I ATGCAT 1 cut(s) 797
FaeI CATG 4 cut(s) 69, 143, 501, 847
FaqI GGGAC 1 cut(s) 698
FatI CATG 4 cut(s) 65, 139, 497, 843
Fnu4HI GCNGC 1 cut(s) 642
FokI GGATG 2 cut(s) 380, 804
Fsp4HI GCNGC 1 cut(s) 642
FspBI CTAG 3 cut(s) 428, 558, 701
GlaI GCGC 1 cut(s) 181
GluI GCNGC 1 cut(s) 642
GsuI CTGGAG 1 cut(s) 513
HaeII RGCGCY 1 cut(s) 183
HaeIII GGCC 2 cut(s) 461, 530
HapII CCGG 1 cut(s) 165
HhaI GCGC 1 cut(s) 182
Hin1II CATG 4 cut(s) 69, 143, 501, 847
Hin6I GCGC 1 cut(s) 180
HinP1I GCGC 1 cut(s) 180
HincII GTYRAC 1 cut(s) 940
HindII GTYRAC 1 cut(s) 940
HindIII AAGCTT 1 cut(s) 308
HinfI GANTC 5 cut(s) 83, 507, 554, 895, 902
HpaI GTTAAC 1 cut(s) 940
HpaII CCGG 1 cut(s) 165
HphI GGTGA 4 cut(s) 125, 274, 858, 900
Hpy166II GTNNAC 3 cut(s) 197, 582, 940
Hpy188I TCNGA 5 cut(s) 155, 291, 327, 916, 951
Hpy188III TCNNGA 4 cut(s) 236, 511, 724, 899
Hpy8I GTNNAC 3 cut(s) 197, 582, 940
HpyAV CCTTC 2 cut(s) 253, 921
HpyCH4III ACNGT 2 cut(s) 256, 517
HpyCH4V TGCA 2 cut(s) 341, 795
HpyF10VI GCNNNNNNNGC 1 cut(s) 647
HpyF3I CTNAG 3 cut(s) 12, 171, 386
Hsp92II CATG 4 cut(s) 69, 143, 501, 847
HspAI GCGC 1 cut(s) 180
KpnI GGTACC 1 cut(s) 662
KspAI GTTAAC 1 cut(s) 940
Kzo9I GATC 5 cut(s) 374, 545, 628, 828, 863
LguI GCTCTTC 1 cut(s) 872
LmnI GCTCC 3 cut(s) 494, 647, 928
Lsp1109I GCAGC 1 cut(s) 653
LweI GCATC 1 cut(s) 782
MaeI CTAG 3 cut(s) 428, 558, 701
MaeIII GTNAC 3 cut(s) 25, 667, 839
MalI GATC 5 cut(s) 376, 547, 630, 830, 865
MboI GATC 5 cut(s) 374, 545, 628, 828, 863
MfeI CAATTG 1 cut(s) 549
MflI RGATCY 2 cut(s) 374, 828
MhlI GDGCHC 1 cut(s) 925
MlsI TGGCCA 1 cut(s) 461
MluCI AATT 8 cut(s) 331, 342, 432, 549, 687, 854, 932, 967
MluNI TGGCCA 1 cut(s) 461
MmeI TCCRAC 2 cut(s) 133, 305
MnlI CCTC 7 cut(s) 65, 292, 395, 433, 675, 762, 952
Mox20I TGGCCA 1 cut(s) 461
Mph1103I ATGCAT 1 cut(s) 797
MroXI GAANNNNTTC 1 cut(s) 201
MscI TGGCCA 1 cut(s) 461
MseI TTAA 4 cut(s) 228, 719, 939, 970
MslI CAYNNNNRTG 1 cut(s) 64
Msp20I TGGCCA 1 cut(s) 461
MspI CCGG 1 cut(s) 165
MspR9I CCNGG 3 cut(s) 165, 177, 399
MunI CAATTG 1 cut(s) 549
Mva1269I GAATGC 1 cut(s) 97
MvaI CCWGG 2 cut(s) 177, 399
MwoI GCNNNNNNNGC 1 cut(s) 647
NciI CCSGG 1 cut(s) 165
NdeII GATC 5 cut(s) 374, 545, 628, 828, 863
NlaIII CATG 4 cut(s) 69, 143, 501, 847
NlaIV GGNNCC 2 cut(s) 660, 830
NmuCI GTSAC 3 cut(s) 25, 667, 839
NsiI ATGCAT 1 cut(s) 797
NspI RCATGY 1 cut(s) 847
PciI ACATGT 1 cut(s) 843
PciSI GCTCTTC 1 cut(s) 872
PctI GAATGC 1 cut(s) 97
PdmI GAANNNNTTC 1 cut(s) 201
PfeI GAWTC 5 cut(s) 83, 507, 554, 895, 902
PkrI GCNGC 1 cut(s) 643
PscI ACATGT 1 cut(s) 843
Psp6I CCWGG 2 cut(s) 175, 397
PspGI CCWGG 2 cut(s) 175, 397
PspN4I GGNNCC 2 cut(s) 660, 830
PspPI GGNCC 1 cut(s) 582
PsuI RGATCY 2 cut(s) 374, 828
RsaI GTAC 3 cut(s) 417, 660, 848
RsaNI GTAC 3 cut(s) 416, 659, 847
RseI CAYNNNNRTG 1 cut(s) 64
SapI GCTCTTC 1 cut(s) 872
SaqAI TTAA 4 cut(s) 228, 719, 939, 970
SatI GCNGC 1 cut(s) 642
Sau3AI GATC 5 cut(s) 374, 545, 628, 828, 863
Sau96I GGNCC 1 cut(s) 582
ScaI AGTACT 1 cut(s) 417
ScrFI CCNGG 3 cut(s) 165, 177, 399
SduI GDGCHC 1 cut(s) 925
SfaNI GCATC 1 cut(s) 782
SfcI CTRYAG 2 cut(s) 750, 780
SinI GGWCC 1 cut(s) 582
SmiMI CAYNNNNRTG 1 cut(s) 64
Sse9I AATT 8 cut(s) 331, 342, 432, 549, 687, 854, 932, 967
SsiI CCGC 1 cut(s) 599
SspMI CTAG 3 cut(s) 428, 558, 701
StyD4I CCNGG 3 cut(s) 163, 175, 397
TaaI ACNGT 2 cut(s) 256, 517
TasI AATT 8 cut(s) 331, 342, 432, 549, 687, 854, 932, 967
TatI WGTACW 2 cut(s) 415, 846
TfiI GAWTC 5 cut(s) 83, 507, 554, 895, 902
Tru1I TTAA 4 cut(s) 228, 719, 939, 970
Tru9I TTAA 4 cut(s) 228, 719, 939, 970
TscAI CASTG 2 cut(s) 85, 261
TseFI GTSAC 3 cut(s) 25, 667, 839
TseI GCWGC 1 cut(s) 641
Tsp45I GTSAC 3 cut(s) 25, 667, 839
TspDTI ATGAA 2 cut(s) 751, 945
TspGWI ACGGA 1 cut(s) 821
TspRI CASTG 2 cut(s) 85, 261
VpaK11BI GGWCC 1 cut(s) 582
XapI RAATTY 2 cut(s) 331, 932
XceI RCATGY 1 cut(s) 847
XcmI CCANNNNNNNNNTGG 1 cut(s) 469
XmnI GAANNNNTTC 1 cut(s) 201
XspI CTAG 3 cut(s) 428, 558, 701
ZrmI AGTACT 1 cut(s) 417
Zsp2I ATGCAT 1 cut(s) 797
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.