Rorug05G0326100

NEMP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0001753
Physical Location & Seq
Reverse (-)
39066816 .. 39068394
1579 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0326100.1

Sequence Viewer

Length: 1401 bp
ATGGCGGAAATGAAACAAAGCATAGTGCTATATCCAGCATCACCTGGATCGCACTACTTGGGTTCCACAGTCGAACTAGGAAAGCTCATCTCCCAACGCCACCCCAACTTCTCCATCACAGTCTTGGTCGATACATCTTCCCCTAAAGCTCTAGCCAATTCCTCATACATCAACCATCTTCTCTCTTCTGATCAAACCTCGAGCTCCATCTCCTTCTTCACTCTCCCCTCCCTCAATCTCCCCCACCCCGACGCCCAAACCAGACCCGAATTCTTCTTAACTGTCCCCAATGTCGTCCAAGCCCTCAAGACAATCTCTCTCACTTCCAAAGTCCTCGCCTTCATCACGTCAGCAGTGCAACACCCTTACGACCCTGAAATCCCAACTTACTATTACTTCCCCTCTTGCGCCTCTGCTCTTGCTCTGTTTCTGTACCTCCCCACTATCCATGATCAAATGCCGGAGAGCTTCAAGGACCTAAACGACGACGTTGTGGAGTTTCCGGGATTGCCAGCGCTCCGAGGCTCGCAAATGCCGGTTTCTATTCTCGACCGCAACGAGGCTGCATATGATCACTATCTCCATATTGCTTCCTGTCTTCCAAAAGCGAAGGGAATTATAATAAACACCATCCAAGAACTTGAGCCTCGAGCAATCAAGGCCATAAAGGAAGGTATTTGTGTTCCTAATCATCGAACCCCGCCGTTATATCACATCGGACCGTTCATTTCCGACTCTGAAAATAGGTCGATCCAAGGCGGTTCGCCTATGCCAACTAACTGTTCCACGTTGTTATGGCTGGATGAGCAGCCGAGCCGGAGTGTGGTGTTCTTGTGCTTTGGAAGCAGAGGTACGTTTTCTGAAGAGCAGTTTAAGGAATTGGCTATAGGCTTGGAGAGAAGCAAGTTGAGATTTTTGTGGGTTGTGAGGAATCAGCTGGATTTGGAGGTACTGCTACCACAAGGGTTCTTGGAGAGGACCAAAGATAGAGGTCTTGTAGTCACGTCTTGGGCGCCACAGGTTGCTATTCTAAGTCATGAATCTGTGGGTGGGTTTGTGACTCACTGTGGGTGGAACTCCATCACTGAAGCAGTGACTTATGGAGTGCCTATGGTGGCTTGGCCTTTGTATTCCGAGCAGGAAGTGAACAGTGTGGTGTTGGTGGAGGAAATGAAGCTGGCAATACCGATATTTGAGGTGCTGTCCAAGTCAAAACAAGGGTTGGTGAGTGCAGATGAGGTGGAGAAGAAGGTGTTGAGGTTGATGGACATGGAGTCGGAAGAAGGGAAAAGTCTAAGAGAAAGGTGTCAAGCTGTCAAGGCAATGGGTTTGGCTGCTTGGAGAAATGGTGGCTCATCCTTCACTGCTTTTTCAGAGCTGGTAAGTTCTTGGATGCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NEMP

Protein Analysis

466

Amino Acids

51.7

Weight (kDa)

5.75

Isoelectric Point (pI)

52.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UDPGT PF00201 270 - 411 2.9e-21 UDP-glucoronosyl and UDP-glucosyl transferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 620
AccB1I GGYRCC 1 cut(s) 1012
AciI CCGC 4 cut(s) 5, 553, 701, 759
AclWI GGATC 2 cut(s) 55, 745
AcsI RAATTY 1 cut(s) 269
AcuI CTGAAG 2 cut(s) 882, 1107
AcyI GRCGYC 2 cut(s) 252, 1013
AfaI GTAC 3 cut(s) 434, 853, 951
AfeI AGCGCT 1 cut(s) 516
AfiI CCNNNNNNNGG 2 cut(s) 559, 823
AgsI TTSAA 1 cut(s) 472
AhdI GACNNNNNGTC 1 cut(s) 1273
AjiI CACGTC 2 cut(s) 348, 1005
AjnI CCWGG 1 cut(s) 43
AjuI GAANNNNNNNTTGG 2 cut(s) 766, 798
AluBI AGCT 8 cut(s) 85, 149, 204, 468, 937, 1177, 1313, 1378
AluI AGCT 8 cut(s) 85, 149, 204, 468, 937, 1177, 1313, 1378
Alw21I GWGCWC 1 cut(s) 206
AlwI GGATC 2 cut(s) 55, 745
Ama87I CYCGRG 2 cut(s) 199, 648
Aor51HI AGCGCT 1 cut(s) 516
AoxI GGCC 2 cut(s) 660, 1121
ApeKI GCWGC 3 cut(s) 563, 808, 1334
ApoI RAATTY 1 cut(s) 269
AspLEI GCGC 3 cut(s) 410, 517, 1015
AspS9I GGNCC 3 cut(s) 475, 719, 978
AsuC2I CCSGG 1 cut(s) 504
AsuHPI GGTGA 2 cut(s) 33, 1237
AvaI CYCGRG 2 cut(s) 199, 648
AvaII GGWCC 3 cut(s) 475, 719, 978
BanI GGYRCC 1 cut(s) 1012
BanII GRGCYC 1 cut(s) 206
BarI GAAGNNNNNNTAC 2 cut(s) 835, 867
BbsI GAAGAC 1 cut(s) 590
Bbv12I GWGCWC 1 cut(s) 206
BbvI GCAGC 3 cut(s) 550, 820, 1321
BccI CCATC 6 cut(s) 122, 183, 215, 638, 1088, 1258
BceAI ACGGC 1 cut(s) 688
BciT130I CCWGG 1 cut(s) 45
BclI TGATCA 3 cut(s) 190, 451, 571
BcnI CCSGG 1 cut(s) 504
BfaI CTAG 2 cut(s) 77, 152
BfmI CTRYAG 1 cut(s) 885
BfoI RGCGCY 2 cut(s) 518, 1016
BisI GCNGC 3 cut(s) 564, 809, 1335
BlsI GCNGC 3 cut(s) 565, 810, 1336
Bme1390I CCNGG 2 cut(s) 45, 504
Bme18I GGWCC 3 cut(s) 475, 719, 978
BmeRI GACNNNNNGTC 1 cut(s) 1273
BmeT110I CYCGRG 2 cut(s) 199, 648
BmgBI CACGTC 2 cut(s) 348, 1005
BmgT120I GGNCC 3 cut(s) 475, 719, 978
BmiI GGNNCC 2 cut(s) 64, 1014
BmrFI CCNGG 2 cut(s) 45, 504
BmsI GCATC 2 cut(s) 47, 1383
BpiI GAAGAC 1 cut(s) 590
BpuEI CTTGAG 2 cut(s) 290, 662
BpuMI CCSGG 1 cut(s) 504
BsaBI GATNNNNATC 1 cut(s) 576
BsaHI GRCGYC 2 cut(s) 252, 1013
BsaJI CCNNGG 2 cut(s) 520, 754
BsaXI ACNNNNNCTCC 2 cut(s) 1333, 1363
Bsc4I CCNNNNNNNGG 2 cut(s) 559, 823
Bse118I RCCGGY 1 cut(s) 535
Bse3DI GCAATG 1 cut(s) 1329
Bse8I GATNNNNATC 1 cut(s) 576
BseBI CCWGG 1 cut(s) 45
BseDI CCNNGG 2 cut(s) 520, 754
BseGI GGATG 4 cut(s) 630, 808, 1355, 1398
BseJI GATNNNNATC 1 cut(s) 576
BseLI CCNNNNNNNGG 2 cut(s) 559, 823
BseMI GCAATG 1 cut(s) 1329
BseXI GCAGC 3 cut(s) 550, 820, 1321
BsgI GTGCAG 1 cut(s) 1251
Bsh1285I CGRYCG 1 cut(s) 553
BshFI GGCC 2 cut(s) 662, 1123
BshNI GGYRCC 1 cut(s) 1012
BsiEI CGRYCG 1 cut(s) 553
BsiHKAI GWGCWC 1 cut(s) 206
BsiHKCI CYCGRG 2 cut(s) 199, 648
BsiSI CCGG 4 cut(s) 461, 503, 536, 817
BslFI GGGAC 1 cut(s) 269
BslI CCNNNNNNNGG 2 cut(s) 559, 823
BsmFI GGGAC 1 cut(s) 269
BsnI GGCC 2 cut(s) 662, 1123
BsoBI CYCGRG 2 cut(s) 199, 648
Bsp1286I GDGCHC 1 cut(s) 206
Bsp143I GATC 5 cut(s) 47, 190, 451, 571, 750
BspACI CCGC 4 cut(s) 5, 553, 701, 759
BspANI GGCC 2 cut(s) 662, 1123
BspHI TCATGA 1 cut(s) 1036
BspLI GGNNCC 2 cut(s) 64, 1014
BspPI GGATC 2 cut(s) 55, 745
BspQI GCTCTTC 1 cut(s) 858
BspT107I GGYRCC 1 cut(s) 1012
BsrDI GCAATG 1 cut(s) 1329
BsrFI RCCGGY 1 cut(s) 535
BssAI RCCGGY 1 cut(s) 535
BssECI CCNNGG 2 cut(s) 520, 754
BssMI GATC 5 cut(s) 47, 190, 451, 571, 750
BssNI GRCGYC 2 cut(s) 252, 1013
BssT1I CCWWGG 1 cut(s) 754
Bst2UI CCWGG 1 cut(s) 45
Bst4CI ACNGT 7 cut(s) 70, 121, 283, 723, 782, 1067, 1151
Bst6I CTCTTC 2 cut(s) 190, 858
BstACI GRCGYC 2 cut(s) 252, 1013
BstC8I GCNNGC 3 cut(s) 513, 527, 1179
BstDEI CTNAG 2 cut(s) 1031, 1295
BstF5I GGATG 4 cut(s) 630, 808, 1355, 1398
BstH2I RGCGCY 2 cut(s) 518, 1016
BstHHI GCGC 3 cut(s) 410, 517, 1015
BstKTI GATC 5 cut(s) 50, 193, 454, 574, 753
BstMBI GATC 5 cut(s) 47, 190, 451, 571, 750
BstMCI CGRYCG 1 cut(s) 553
BstMWI GCNNNNNNNGC 4 cut(s) 659, 805, 843, 1319
BstNI CCWGG 1 cut(s) 45
BstSCI CCNGG 2 cut(s) 43, 502
BstSFI CTRYAG 1 cut(s) 885
BstV1I GCAGC 3 cut(s) 550, 820, 1321
BstV2I GAAGAC 1 cut(s) 590
BsuRI GGCC 2 cut(s) 662, 1123
BtrI CACGTC 2 cut(s) 348, 1005
BtsCI GGATG 4 cut(s) 630, 808, 1355, 1398
BtsI GCAGTG 3 cut(s) 360, 1098, 1362
BtsIMutI CAGTG 6 cut(s) 360, 1063, 1083, 1098, 1156, 1362
Cac8I GCNNGC 3 cut(s) 513, 527, 1179
CciI TCATGA 1 cut(s) 1036
CfoI GCGC 3 cut(s) 410, 517, 1015
Cfr10I RCCGGY 1 cut(s) 535
Cfr13I GGNCC 3 cut(s) 475, 719, 978
CpoI CGGWCCG 1 cut(s) 719
CseI GACGC 1 cut(s) 260
Csp6I GTAC 3 cut(s) 433, 852, 950
CspI CGGWCCG 1 cut(s) 719
CviAII CATG 3 cut(s) 449, 1037, 1270
CviQI GTAC 3 cut(s) 433, 852, 950
DdeI CTNAG 2 cut(s) 1031, 1295
DinI GGCGCC 1 cut(s) 1014
DpnI GATC 5 cut(s) 49, 192, 453, 573, 752
DpnII GATC 5 cut(s) 47, 190, 451, 571, 750
DriI GACNNNNNGTC 1 cut(s) 1273
Eam1104I CTCTTC 2 cut(s) 190, 858
Eam1105I GACNNNNNGTC 1 cut(s) 1273
EarI CTCTTC 2 cut(s) 190, 858
EciI GGCGGA 1 cut(s) 20
Ecl136II GAGCTC 1 cut(s) 204
Eco130I CCWWGG 1 cut(s) 754
Eco24I GRGCYC 1 cut(s) 206
Eco47I GGWCC 3 cut(s) 475, 719, 978
Eco47III AGCGCT 1 cut(s) 516
Eco53kI GAGCTC 1 cut(s) 204
Eco57I CTGAAG 2 cut(s) 882, 1107
Eco88I CYCGRG 2 cut(s) 199, 648
EcoICRI GAGCTC 1 cut(s) 204
EcoO109I RGGNCCY 1 cut(s) 475
EcoRI GAATTC 1 cut(s) 269
EcoRII CCWGG 1 cut(s) 43
EcoT14I CCWWGG 1 cut(s) 754
EcoT38I GRGCYC 1 cut(s) 206
EgeI GGCGCC 1 cut(s) 1014
EheI GGCGCC 1 cut(s) 1014
ErhI CCWWGG 1 cut(s) 754
FaeI CATG 3 cut(s) 452, 1040, 1273
FaqI GGGAC 1 cut(s) 269
FatI CATG 3 cut(s) 448, 1036, 1269
FauI CCCGC 1 cut(s) 708
FauNDI CATATG 1 cut(s) 568
FbaI TGATCA 3 cut(s) 190, 451, 571
Fnu4HI GCNGC 3 cut(s) 564, 809, 1335
FokI GGATG 3 cut(s) 617, 815, 1342
FriOI GRGCYC 1 cut(s) 206
Fsp4HI GCNGC 3 cut(s) 564, 809, 1335
FspBI CTAG 2 cut(s) 77, 152
GlaI GCGC 3 cut(s) 409, 516, 1014
GluI GCNGC 3 cut(s) 564, 809, 1335
HaeII RGCGCY 2 cut(s) 518, 1016
HaeIII GGCC 2 cut(s) 662, 1123
HapII CCGG 4 cut(s) 461, 503, 536, 817
HgaI GACGC 1 cut(s) 260
HhaI GCGC 3 cut(s) 410, 517, 1015
Hin1I GRCGYC 2 cut(s) 252, 1013
Hin1II CATG 3 cut(s) 452, 1040, 1273
Hin6I GCGC 3 cut(s) 408, 515, 1013
HinP1I GCGC 3 cut(s) 408, 515, 1013
HinfI GANTC 5 cut(s) 734, 931, 1040, 1060, 1274
HpaII CCGG 4 cut(s) 461, 503, 536, 817
HphI GGTGA 2 cut(s) 33, 1237
Hpy166II GTNNAC 1 cut(s) 1147
Hpy188I TCNGA 9 cut(s) 190, 521, 719, 733, 739, 862, 1135, 1279, 1375
Hpy188III TCNNGA 3 cut(s) 307, 548, 1037
Hpy8I GTNNAC 1 cut(s) 1147
Hpy99I CGWCG 3 cut(s) 254, 488, 491
HpyAV CCTTC 7 cut(s) 223, 349, 604, 665, 1243, 1277, 1369
HpyCH4III ACNGT 7 cut(s) 70, 121, 283, 723, 782, 1067, 1151
HpyCH4IV ACGT 5 cut(s) 347, 489, 788, 854, 1004
HpyCH4V TGCA 4 cut(s) 358, 566, 1232, 1396
HpyF10VI GCNNNNNNNGC 4 cut(s) 659, 805, 843, 1319
HpyF3I CTNAG 2 cut(s) 1031, 1295
HpySE526I ACGT 5 cut(s) 347, 489, 788, 854, 1004
Hsp92I GRCGYC 2 cut(s) 252, 1013
Hsp92II CATG 3 cut(s) 452, 1040, 1273
HspAI GCGC 3 cut(s) 408, 515, 1013
KasI GGCGCC 1 cut(s) 1012
Ksp22I TGATCA 3 cut(s) 190, 451, 571
Kzo9I GATC 5 cut(s) 47, 190, 451, 571, 750
LguI GCTCTTC 1 cut(s) 858
LmnI GCTCC 2 cut(s) 209, 522
Lsp1109I GCAGC 3 cut(s) 550, 820, 1321
LweI GCATC 2 cut(s) 47, 1383
MaeI CTAG 2 cut(s) 77, 152
MaeII ACGT 5 cut(s) 347, 489, 788, 854, 1004
MaeIII GTNAC 3 cut(s) 1000, 1057, 1093
MalI GATC 5 cut(s) 49, 192, 453, 573, 752
MboI GATC 5 cut(s) 47, 190, 451, 571, 750
MboII GAAGA 9 cut(s) 129, 170, 177, 208, 265, 590, 875, 1258, 1292
MhlI GDGCHC 1 cut(s) 206
MluCI AATT 4 cut(s) 157, 269, 615, 878
Mly113I GGCGCC 1 cut(s) 1013
MlyI GAGTC 3 cut(s) 728, 1054, 1283
MmeI TCCRAC 2 cut(s) 756, 1257
MseI TTAA 2 cut(s) 278, 873
MspA1I CMGCKG 1 cut(s) 937
MspI CCGG 4 cut(s) 461, 503, 536, 817
MspR9I CCNGG 2 cut(s) 45, 504
MvaI CCWGG 1 cut(s) 45
MwoI GCNNNNNNNGC 4 cut(s) 659, 805, 843, 1319
NarI GGCGCC 1 cut(s) 1013
NciI CCSGG 1 cut(s) 504
NdeI CATATG 1 cut(s) 568
NdeII GATC 5 cut(s) 47, 190, 451, 571, 750
NlaIII CATG 3 cut(s) 452, 1040, 1273
NlaIV GGNNCC 2 cut(s) 64, 1014
NmeAIII GCCGAG 1 cut(s) 837
NmuCI GTSAC 3 cut(s) 1000, 1057, 1093
PaeR7I CTCGAG 2 cut(s) 199, 648
PagI TCATGA 1 cut(s) 1036
PciSI GCTCTTC 1 cut(s) 858
PcsI WCGNNNNNNNCGW 1 cut(s) 555
PfeI GAWTC 2 cut(s) 931, 1040
PfoI TCCNGGA 1 cut(s) 502
PkrI GCNGC 3 cut(s) 565, 810, 1336
PleI GAGTC 3 cut(s) 728, 1054, 1282
PluTI GGCGCC 1 cut(s) 1016
PpsI GAGTC 3 cut(s) 728, 1054, 1282
PpuMI RGGWCCY 1 cut(s) 475
PsiI TTATAA 1 cut(s) 620
Psp124BI GAGCTC 1 cut(s) 206
Psp5II RGGWCCY 1 cut(s) 475
Psp6I CCWGG 1 cut(s) 43
PspGI CCWGG 1 cut(s) 43
PspN4I GGNNCC 2 cut(s) 64, 1014
PspPI GGNCC 3 cut(s) 475, 719, 978
PspPPI RGGWCCY 1 cut(s) 475
PspXI VCTCGAGB 2 cut(s) 199, 648
PvuII CAGCTG 1 cut(s) 937
RsaI GTAC 3 cut(s) 434, 853, 951
RsaNI GTAC 3 cut(s) 433, 852, 950
Rsr2I CGGWCCG 1 cut(s) 719
RsrII CGGWCCG 1 cut(s) 719
SacI GAGCTC 1 cut(s) 206
SapI GCTCTTC 1 cut(s) 858
SaqAI TTAA 2 cut(s) 278, 873
SatI GCNGC 3 cut(s) 564, 809, 1335
Sau3AI GATC 5 cut(s) 47, 190, 451, 571, 750
Sau96I GGNCC 3 cut(s) 475, 719, 978
SchI GAGTC 3 cut(s) 728, 1054, 1283
ScrFI CCNGG 2 cut(s) 45, 504
SduI GDGCHC 1 cut(s) 206
SfaNI GCATC 2 cut(s) 47, 1383
SfcI CTRYAG 1 cut(s) 885
SfoI GGCGCC 1 cut(s) 1014
Sfr274I CTCGAG 2 cut(s) 199, 648
SinI GGWCC 3 cut(s) 475, 719, 978
SlaI CTCGAG 2 cut(s) 199, 648
SmlI CTYRAG 4 cut(s) 199, 305, 641, 648
SmoI CTYRAG 4 cut(s) 199, 305, 641, 648
Sse9I AATT 4 cut(s) 157, 269, 615, 878
SsiI CCGC 4 cut(s) 5, 553, 701, 759
SspDI GGCGCC 1 cut(s) 1012
SspMI CTAG 2 cut(s) 77, 152
SstI GAGCTC 1 cut(s) 206
StyD4I CCNGG 2 cut(s) 43, 502
StyI CCWWGG 1 cut(s) 754
TaaI ACNGT 7 cut(s) 70, 121, 283, 723, 782, 1067, 1151
TaiI ACGT 5 cut(s) 350, 492, 791, 857, 1007
TaqI TCGA 7 cut(s) 72, 129, 200, 549, 649, 694, 749
TasI AATT 4 cut(s) 157, 269, 615, 878
TfiI GAWTC 2 cut(s) 931, 1040
Tru1I TTAA 2 cut(s) 278, 873
Tru9I TTAA 2 cut(s) 278, 873
TscAI CASTG 6 cut(s) 360, 1070, 1090, 1098, 1156, 1369
TseFI GTSAC 3 cut(s) 1000, 1057, 1093
TseI GCWGC 3 cut(s) 563, 808, 1334
Tsp45I GTSAC 3 cut(s) 1000, 1057, 1093
TspDTI ATGAA 5 cut(s) 26, 331, 715, 1053, 1187
TspRI CASTG 6 cut(s) 360, 1070, 1090, 1098, 1156, 1369
VpaK11BI GGWCC 3 cut(s) 475, 719, 978
XapI RAATTY 1 cut(s) 269
XcmI CCANNNNNNNNNTGG 1 cut(s) 121
XhoI CTCGAG 2 cut(s) 199, 648
XspI CTAG 2 cut(s) 77, 152
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.