RchiOBHm_Chr5g0059811

NEMP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0001753
Physical Location & Seq
Forward (+)
64900982 .. 64901449
468 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ33639

Sequence Viewer

Length: 465 bp
ATGATTTTCCTTCTTCAGGTAATGAAGCTTCTCCCAACTGGTCGGAAGAATTCACTCGCAATTTTCGTCTTGTCATCTGTGGTTGGTTTGGGATCTTTTCTCCTCCGCTACATCCTAGGACTACTAAGTTCAGTACTCATAGAGCTTGGAATTAGTGAGGATATGTATAATGTTTTGGCCATCTTTCTTCTGGCTTTTGTTTTTCCGGCTGGAGCGTCGTTGGGATTCTGGACAGTTTGTAAAATGGCCTTGAACGAAGATGGATCAATTGATTCTCGCACATCTCTGTTTGTTTTGTGGACCATTCGCTTCGTAGCTGTAATTTTTCTTTCACAGAGTTCCAGCTATCAACTACTGGCAGCAGGAGCTATAATGGTACATTTTTTTGAGAAAATAGATAACTTCATTACTTATCTATGGTCAGAAGGCACGTATATCATAAGTTGTCTCATACCAAATTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NEMP

Protein Analysis

154

Amino Acids

17.08

Weight (kDa)

5.68

Isoelectric Point (pI)

39.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NEMP PF10225 1 - 132 1.4e-14 NEMP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 106
AclWI GGATC 2 cut(s) 100, 271
AcoI YGGCCR 1 cut(s) 177
AcsI RAATTY 2 cut(s) 49, 457
AfaI GTAC 2 cut(s) 135, 378
AfiI CCNNNNNNNGG 1 cut(s) 16
AgsI TTSAA 1 cut(s) 253
AluBI AGCT 5 cut(s) 28, 145, 317, 345, 368
AluI AGCT 5 cut(s) 28, 145, 317, 345, 368
Alw26I GTCTC 1 cut(s) 452
AlwI GGATC 2 cut(s) 100, 271
AoxI GGCC 2 cut(s) 177, 246
ApeKI GCWGC 1 cut(s) 359
ApoI RAATTY 2 cut(s) 49, 457
AspA2I CCTAGG 1 cut(s) 115
AspS9I GGNCC 1 cut(s) 300
AvaII GGWCC 1 cut(s) 300
AvrII CCTAGG 1 cut(s) 115
BalI TGGCCA 1 cut(s) 179
BarI GAAGNNNNNNTAC 2 cut(s) 12, 44
BbvI GCAGC 1 cut(s) 371
BccI CCATC 2 cut(s) 188, 254
BcoDI GTCTC 1 cut(s) 452
BfaI CTAG 1 cut(s) 116
BisI GCNGC 1 cut(s) 360
BlnI CCTAGG 1 cut(s) 115
BlsI GCNGC 1 cut(s) 361
BmcAI AGTACT 1 cut(s) 135
Bme18I GGWCC 1 cut(s) 300
BmgT120I GGNCC 1 cut(s) 300
BpmI CTGGAG 1 cut(s) 231
BsaAI YACGTR 1 cut(s) 432
BsaJI CCNNGG 1 cut(s) 115
Bsc4I CCNNNNNNNGG 1 cut(s) 16
Bse1I ACTGG 2 cut(s) 43, 360
BseDI CCNNGG 1 cut(s) 115
BseGI GGATG 1 cut(s) 111
BseLI CCNNNNNNNGG 1 cut(s) 16
BseNI ACTGG 2 cut(s) 43, 360
BseRI GAGGAG 1 cut(s) 92
BseXI GCAGC 1 cut(s) 371
BshFI GGCC 2 cut(s) 179, 248
BsiSI CCGG 1 cut(s) 206
BslI CCNNNNNNNGG 1 cut(s) 16
BsmAI GTCTC 1 cut(s) 452
BsnI GGCC 2 cut(s) 179, 248
Bsp143I GATC 2 cut(s) 92, 263
BspACI CCGC 1 cut(s) 106
BspANI GGCC 2 cut(s) 179, 248
BspPI GGATC 2 cut(s) 100, 271
BsrI ACTGG 2 cut(s) 43, 360
BssECI CCNNGG 1 cut(s) 115
BssMI GATC 2 cut(s) 92, 263
BssT1I CCWWGG 1 cut(s) 115
Bst4CI ACNGT 1 cut(s) 235
BstBAI YACGTR 1 cut(s) 432
BstDEI CTNAG 1 cut(s) 125
BstENI CCTNNNNNAGG 1 cut(s) 14
BstF5I GGATG 1 cut(s) 111
BstKTI GATC 2 cut(s) 95, 266
BstMAI GTCTC 1 cut(s) 452
BstMBI GATC 2 cut(s) 92, 263
BstMWI GCNNNNNNNGC 1 cut(s) 365
BstV1I GCAGC 1 cut(s) 371
BstX2I RGATCY 1 cut(s) 92
BstYI RGATCY 1 cut(s) 92
BsuRI GGCC 2 cut(s) 179, 248
BtsCI GGATG 1 cut(s) 111
Cfr13I GGNCC 1 cut(s) 300
CseI GACGC 1 cut(s) 204
Csp6I GTAC 2 cut(s) 134, 377
CviJI RGCY 9 cut(s) 28, 145, 179, 194, 209, 248, 317, 345, 368
CviKI_1 RGCY 9 cut(s) 28, 145, 179, 194, 209, 248, 317, 345, 368
CviQI GTAC 2 cut(s) 134, 377
DdeI CTNAG 1 cut(s) 125
DpnI GATC 2 cut(s) 94, 265
DpnII GATC 2 cut(s) 92, 263
EaeI YGGCCR 1 cut(s) 177
Eco130I CCWWGG 1 cut(s) 115
Eco47I GGWCC 1 cut(s) 300
EcoNI CCTNNNNNAGG 1 cut(s) 14
EcoRI GAATTC 1 cut(s) 49
EcoT14I CCWWGG 1 cut(s) 115
ErhI CCWWGG 1 cut(s) 115
FaiI YATR 8 cut(s) 140, 164, 168, 371, 418, 435, 440, 452
Fnu4HI GCNGC 1 cut(s) 360
FokI GGATG 1 cut(s) 98
Fsp4HI GCNGC 1 cut(s) 360
FspBI CTAG 1 cut(s) 116
GluI GCNGC 1 cut(s) 360
GsuI CTGGAG 1 cut(s) 231
HaeIII GGCC 2 cut(s) 179, 248
HapII CCGG 1 cut(s) 206
HgaI GACGC 1 cut(s) 204
HindIII AAGCTT 1 cut(s) 26
HinfI GANTC 2 cut(s) 225, 272
HpaII CCGG 1 cut(s) 206
Hpy166II GTNNAC 1 cut(s) 300
Hpy188I TCNGA 2 cut(s) 45, 424
Hpy188III TCNNGA 2 cut(s) 229, 462
Hpy8I GTNNAC 1 cut(s) 300
Hpy99I CGWCG 1 cut(s) 220
HpyAV CCTTC 2 cut(s) 20, 419
HpyCH4III ACNGT 1 cut(s) 235
HpyCH4IV ACGT 1 cut(s) 431
HpyF10VI GCNNNNNNNGC 1 cut(s) 365
HpyF3I CTNAG 1 cut(s) 125
HpySE526I ACGT 1 cut(s) 431
Kzo9I GATC 2 cut(s) 92, 263
LmnI GCTCC 2 cut(s) 212, 365
LpnPI CCDG 9 cut(s) 2, 24, 176, 195, 214, 219, 341, 348, 355
Lsp1109I GCAGC 1 cut(s) 371
MaeI CTAG 1 cut(s) 116
MaeII ACGT 1 cut(s) 431
MalI GATC 2 cut(s) 94, 265
MboI GATC 2 cut(s) 92, 263
MboII GAAGA 4 cut(s) 5, 58, 179, 269
MfeI CAATTG 1 cut(s) 267
MflI RGATCY 1 cut(s) 92
MlsI TGGCCA 1 cut(s) 179
MluCI AATT 6 cut(s) 49, 60, 150, 267, 321, 457
MluNI TGGCCA 1 cut(s) 179
MmeI TCCRAC 1 cut(s) 23
MnlI CCTC 2 cut(s) 113, 151
Mox20I TGGCCA 1 cut(s) 179
MscI TGGCCA 1 cut(s) 179
Msp20I TGGCCA 1 cut(s) 179
MspI CCGG 1 cut(s) 206
MunI CAATTG 1 cut(s) 267
MwoI GCNNNNNNNGC 1 cut(s) 365
NdeII GATC 2 cut(s) 92, 263
PcsI WCGNNNNNNNCGW 1 cut(s) 63
PfeI GAWTC 2 cut(s) 225, 272
PkrI GCNGC 1 cut(s) 361
Ppu21I YACGTR 1 cut(s) 432
PspPI GGNCC 1 cut(s) 300
PsuI RGATCY 1 cut(s) 92
RsaI GTAC 2 cut(s) 135, 378
RsaNI GTAC 2 cut(s) 134, 377
SatI GCNGC 1 cut(s) 360
Sau3AI GATC 2 cut(s) 92, 263
Sau96I GGNCC 1 cut(s) 300
ScaI AGTACT 1 cut(s) 135
SetI ASST 7 cut(s) 21, 30, 147, 319, 347, 370, 434
SinI GGWCC 1 cut(s) 300
Sse9I AATT 6 cut(s) 49, 60, 150, 267, 321, 457
SsiI CCGC 1 cut(s) 106
SspMI CTAG 1 cut(s) 116
StyI CCWWGG 1 cut(s) 115
TaaI ACNGT 1 cut(s) 235
TaiI ACGT 1 cut(s) 434
TasI AATT 6 cut(s) 49, 60, 150, 267, 321, 457
TatI WGTACW 1 cut(s) 133
TfiI GAWTC 2 cut(s) 225, 272
TseI GCWGC 1 cut(s) 359
TspDTI ATGAA 2 cut(s) 38, 394
VpaK11BI GGWCC 1 cut(s) 300
XagI CCTNNNNNAGG 1 cut(s) 14
XapI RAATTY 2 cut(s) 49, 457
XcmI CCANNNNNNNNNTGG 1 cut(s) 187
XmaJI CCTAGG 1 cut(s) 115
XspI CTAG 1 cut(s) 116
ZrmI AGTACT 1 cut(s) 135
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.