FvH4_1g02190

Pyrophosphate-energized vacuolar membrane proton

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0000897
Physical Location & Seq
Reverse (-)
1171413 .. 1176295
4883 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g02190.t2

Sequence Viewer

Length: 2001 bp
ATGGTGGCATTTGCAATCTTGATATTCGTTTTCCTTGGCTCTGTGGAGGGATTTAGCACTAGTGGCCATAAATGTGCTAATGACGAAACAAAGATGTGCAAACCTGCTCTTGCAACTGCCTTATTCAGCACTGTTTCCTTTTTGCTTGGTGCTATAACTTCAGTGGTTTCTGGATTCCTTGGTATGAAGATTGCTACTTATGCAAATGCCAGAACTACTTTGGAGGCAAGAAAAGGTGTTGGGAAGGCTTTTATAGTTGCATTTAGATCAGGTGCTGTTATGGGATTTCTCCTTGCTGCAAATGGTTTATTGGTTCTTTACATTGTGATCCTTGTCTTCAAGATGTACTATGGTGAGGACTGGGGTGGTCTTTTTGAGTCCATAACTGGTTATGGTCTTGGCGGATCTTCTATGGCTCTCTTTGGCAGAGTTGGTGGAGGCATCTATACCAAAGCTGCTGATGTTGGTGCTGACCTTGTTGGCAAAGTTGAAAGGAATATTCCTGAGGATGACCCAAGGAACCCAGCTGTGATTGCTGATAATGTTGGGGACAATGTTGGGGATATTGCCGGAATGGGATCTGACCTCTTTGGATCATATGCTGAGTCATCATGTGCTGCTCTTGTTGTTGCTTCAATTTCCTCATTTGGGATCAATCATGAGTTAACTGCAATGTTATATCCTCTCATCGTCAGTTCCATTGGTATCATTGTTTGTCTGATCACCACCCTTTTCGCAACTGATTTCTTTGAGATCAAGGCTGTAAAGGAAATTGAGCCAGCATTGAAGAGGCAACTCATTATTTCCACTGTACTGATGACTGTTGGAGTTGCGATCGTTACATGGATTTCTGTTCCTTCTTCCTTCACCATCTTTAATTTTGGAACCCAGAAAGTTGTTAAGAACTGGCAACTGTTCTTATGTGTGTGTGTGGGTTTGTGGGCTGGACTTATCATCGGATTTGTAACAGAGTATTATACCAGTAATGCGTACAGCCCCGTGCAAGATGTTGCTGATTCGTGCCGGACAGGAGCTGCTACTAATGTCATTTTTGGCCTGGCATTGGGATACAAATCAGTCATCATTCCTATTTTTGCCATTGCCATTAGTATTTATGTTAGTTTTAGCTTTGCCGCTATGTATGGTATTGCTATAGCTGCCCTTGGAATGCTTAGCACCATAGCGACTGGATTGGCCATTGATGCATATGGTCCGATCAGTGACAATGCTGGAGGTATTGCTGAGATGGCAGGCATGAGCCACAGAATCAGAGAGAGAACGGATGCTCTTGATGCTGCAGGAAACACAACAGCTGCTATTGGAAAGGGTTTTGCAATTGGCTCTGCTGCTCTCGTGTCCCTTGCTCTCTTTGGTGCCTTTGTGAGCCGTGCTGGAATTGCAACAGTTGATGTGTTGACTCCAAAAGTGTTTATTGGTTTGATTGTGGGTGCAATGCTTCCCTACTGGTTCTCTGCCATGACAATGAAGAGTGTGGGAAGTGCTGCTCTGAAAATGGTTGAAGAAGTACGCAGGCAATTCAACACCATCCCTGGCCTCATGGAGGGTACTGCCAAGCCTGACTATGCTACGTGTGTTAAGATCTCTACTGATGCTTCCATCAAGGAAATGATTCCACCTGGTGCCCTTGTCATGCTTACGCCCCTCATCGTCGGGATCTTTTTTGGTGTGGAGACTCTCTCCGGTGTCCTTGCTGGATCCCTCGTCTCTGGTGTACAGATCGCAATCTCTGCATCCAACACTGGTGGTGCTTGGGACAACGCCAAGAAGTATATTGAGGCTGGAGCTTCTGAACACGCAAGGAGTCTTGGTCCCAAGGGTTCAGATCCACACAAAGCAGCTGTCATTGGTGACACTGTTGGAGACCCTCTCAAGGACACATCTGGCCCATCTCTCAACATCCTCATCAAGCTCATGGCCGTCGAGTCCCTTGTCTTTGCACCTTTCTTCGCCACACACGGTGGCCTCCTTTTCAAGCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 map00190
Pfam Domains
Protein Families
H_PPase

Protein Analysis

667

Amino Acids

69.4

Weight (kDa)

5.96

Isoelectric Point (pI)

20.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
H_PPase PF03030 65 - 751 4.9e-258 Inorganic H+ pyrophosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 112
AccB1I GGYRCC 2 cut(s) 1373, 1640
AciI CCGC 2 cut(s) 402, 1134
AclWI GGATC 9 cut(s) 322, 412, 586, 601, 659, 1682, 1710, 1723, 1838
AcoI YGGCCR 3 cut(s) 64, 1194, 1935
AcuI CTGAAG 1 cut(s) 144
AdeI CACNNNGTG 2 cut(s) 1640, 1979
AfaI GTAC 6 cut(s) 347, 813, 992, 1527, 1567, 1734
AfiI CCNNNNNNNGG 4 cut(s) 648, 1063, 1561, 1891
AflIII ACRYGT 1 cut(s) 1589
AgsI TTSAA 7 cut(s) 340, 491, 636, 787, 1520, 1540, 1993
AhlI ACTAGT 1 cut(s) 59
AjnI CCWGG 3 cut(s) 1056, 1549, 1636
Alw26I GTCTC 3 cut(s) 1685, 1729, 1875
AlwI GGATC 9 cut(s) 322, 412, 586, 601, 659, 1682, 1710, 1723, 1838
AlwNI CAGNNNCTG 2 cut(s) 275, 1034
AoxI GGCC 7 cut(s) 64, 1054, 1194, 1552, 1903, 1935, 1981
ArsI GACNNNNNNTTYG 2 cut(s) 1845, 1877
Asp700I GAANNNNTTC 1 cut(s) 1629
AspS9I GGNCC 3 cut(s) 1211, 1829, 1904
AsuHPI GGTGA 4 cut(s) 365, 715, 859, 1880
AvaII GGWCC 2 cut(s) 1211, 1829
AxyI CCTNAGG 1 cut(s) 504
BaeGI GKGCMC 1 cut(s) 1645
BalI TGGCCA 2 cut(s) 66, 1196
BamHI GGATCC 1 cut(s) 1715
BanI GGYRCC 2 cut(s) 1373, 1640
BauI CACGAG 1 cut(s) 1352
BbsI GAAGAC 1 cut(s) 328
BccI CCATC 5 cut(s) 878, 1240, 1553, 1625, 1915
BceAI ACGGC 2 cut(s) 1371, 1922
BciT130I CCWGG 3 cut(s) 1058, 1551, 1638
BciVI GTATCC 1 cut(s) 1061
BclI TGATCA 1 cut(s) 720
BcoDI GTCTC 3 cut(s) 1685, 1729, 1875
BcuI ACTAGT 1 cut(s) 59
BfaI CTAG 1 cut(s) 60
BfmI CTRYAG 2 cut(s) 1152, 1296
BfuAI ACCTGC 1 cut(s) 112
BfuI GTATCC 1 cut(s) 1061
BglII AGATCT 1 cut(s) 1599
BlpI GCTNAGC 1 cut(s) 1172
Bme1390I CCNGG 3 cut(s) 1058, 1551, 1638
Bme18I GGWCC 2 cut(s) 1211, 1829
BmgT120I GGNCC 3 cut(s) 1211, 1829, 1904
BmiI GGNNCC 6 cut(s) 521, 886, 1375, 1642, 1717, 1831
BmrFI CCNGG 3 cut(s) 1058, 1551, 1638
BmrI ACTGGG 1 cut(s) 370
BmsI GCATC 6 cut(s) 450, 1192, 1273, 1282, 1600, 1760
BmuI ACTGGG 1 cut(s) 370
BpiI GAAGAC 1 cut(s) 328
BplI GAGNNNNNCTC 4 cut(s) 1682, 1714, 1872, 1904
BpmI CTGGAG 2 cut(s) 1251, 1821
Bpu1102I GCTNAGC 1 cut(s) 1172
BpuEI CTTGAG 1 cut(s) 1874
BsaAI YACGTR 1 cut(s) 1590
BsaBI GATNNNNATC 2 cut(s) 1072, 1742
BsaI GGTCTC 1 cut(s) 1875
BsaJI CCNNGG 6 cut(s) 34, 178, 515, 1162, 1549, 1833
BsaWI WCCGGW 1 cut(s) 1700
BsaXI ACNNNNNCTCC 2 cut(s) 1813, 1843
Bsc4I CCNNNNNNNGG 4 cut(s) 648, 1063, 1561, 1891
Bse1I ACTGG 7 cut(s) 365, 391, 911, 981, 1192, 1469, 1765
Bse21I CCTNAGG 1 cut(s) 504
Bse3DI GCAATG 3 cut(s) 678, 1098, 1458
Bse8I GATNNNNATC 2 cut(s) 1072, 1742
BseBI CCWGG 3 cut(s) 1058, 1551, 1638
BseDI CCNNGG 6 cut(s) 34, 178, 515, 1162, 1549, 1833
BseGI GGATG 5 cut(s) 514, 1288, 1545, 1751, 1917
BseJI GATNNNNATC 2 cut(s) 1072, 1742
BseLI CCNNNNNNNGG 4 cut(s) 648, 1063, 1561, 1891
BseMI GCAATG 3 cut(s) 678, 1098, 1458
BseMII CTCAG 3 cut(s) 495, 594, 1233
BseNI ACTGG 7 cut(s) 365, 391, 911, 981, 1192, 1469, 1765
BseSI GKGCMC 1 cut(s) 1645
BseYI CCCAGC 1 cut(s) 523
Bsh1285I CGRYCG 1 cut(s) 837
BshFI GGCC 7 cut(s) 66, 1056, 1196, 1554, 1905, 1937, 1983
BshNI GGYRCC 2 cut(s) 1373, 1640
BsiEI CGRYCG 1 cut(s) 837
BsiSI CCGG 3 cut(s) 570, 1024, 1701
BslFI GGGAC 5 cut(s) 563, 1342, 1787, 1815, 1930
BslI CCNNNNNNNGG 4 cut(s) 648, 1063, 1561, 1891
BsmAI GTCTC 3 cut(s) 1685, 1729, 1875
BsmBI CGTCTC 1 cut(s) 1729
BsmFI GGGAC 5 cut(s) 563, 1342, 1787, 1815, 1930
BsmI GAATGC 1 cut(s) 1173
BsnI GGCC 7 cut(s) 66, 1056, 1196, 1554, 1905, 1937, 1983
Bso31I GGTCTC 1 cut(s) 1875
Bsp1286I GDGCHC 1 cut(s) 1645
Bsp1407I TGTACA 1 cut(s) 1732
Bsp1720I GCTNAGC 1 cut(s) 1172
BspACI CCGC 2 cut(s) 402, 1134
BspANI GGCC 7 cut(s) 66, 1056, 1196, 1554, 1905, 1937, 1983
BspCNI CTCAG 3 cut(s) 496, 595, 1234
BspHI TCATGA 1 cut(s) 658
BspLI GGNNCC 6 cut(s) 521, 886, 1375, 1642, 1717, 1831
BspMAI CTGCAG 1 cut(s) 1300
BspMI ACCTGC 1 cut(s) 112
BspPI GGATC 9 cut(s) 322, 412, 586, 601, 659, 1682, 1710, 1723, 1838
BspT107I GGYRCC 2 cut(s) 1373, 1640
BspTNI GGTCTC 1 cut(s) 1875
BsrDI GCAATG 3 cut(s) 678, 1098, 1458
BsrGI TGTACA 1 cut(s) 1732
BsrI ACTGG 7 cut(s) 365, 391, 911, 981, 1192, 1469, 1765
BssECI CCNNGG 6 cut(s) 34, 178, 515, 1162, 1549, 1833
BssSI CACGAG 1 cut(s) 1352
BssT1I CCWWGG 5 cut(s) 34, 178, 515, 1162, 1833
Bst2BI CACGAG 1 cut(s) 1352
Bst2UI CCWGG 3 cut(s) 1058, 1551, 1638
Bst4CI ACNGT 7 cut(s) 133, 811, 823, 915, 1405, 1876, 1979
Bst6I CTCTTC 2 cut(s) 782, 1481
BstAPI GCANNNNNTGC 1 cut(s) 1748
BstAUI TGTACA 1 cut(s) 1732
BstBAI YACGTR 1 cut(s) 1590
BstC8I GCNNGC 3 cut(s) 780, 1252, 1532
BstDEI CTNAG 4 cut(s) 504, 603, 1172, 1242
BstENI CCTNNNNNAGG 1 cut(s) 1559
BstF5I GGATG 5 cut(s) 514, 1288, 1545, 1751, 1917
BstMAI GTCTC 3 cut(s) 1685, 1729, 1875
BstMCI CGRYCG 1 cut(s) 837
BstMWI GCNNNNNNNGC 9 cut(s) 63, 200, 533, 1157, 1202, 1247, 1292, 1397, 1748
BstNI CCWGG 3 cut(s) 1058, 1551, 1638
BstSCI CCNGG 3 cut(s) 1056, 1549, 1636
BstSFI CTRYAG 2 cut(s) 1152, 1296
BstSLI GKGCMC 1 cut(s) 1645
BstV2I GAAGAC 1 cut(s) 328
BstX2I RGATCY 6 cut(s) 404, 578, 1599, 1674, 1715, 1843
BstYI RGATCY 6 cut(s) 404, 578, 1599, 1674, 1715, 1843
Bsu36I CCTNAGG 1 cut(s) 504
BsuI GTATCC 1 cut(s) 1061
BsuRI GGCC 7 cut(s) 66, 1056, 1196, 1554, 1905, 1937, 1983
BtsCI GGATG 5 cut(s) 514, 1288, 1545, 1751, 1917
BtsIMutI CAGTG 6 cut(s) 129, 168, 807, 1225, 1758, 1872
BveI ACCTGC 1 cut(s) 112
Cac8I GCNNGC 3 cut(s) 780, 1252, 1532
CaiI CAGNNNCTG 2 cut(s) 275, 1034
CciI TCATGA 1 cut(s) 658
Cfr13I GGNCC 3 cut(s) 1211, 1829, 1904
CsiI ACCWGGT 1 cut(s) 1636
Csp6I GTAC 6 cut(s) 346, 812, 991, 1526, 1566, 1733
CspCI CAANNNNNGTGG 2 cut(s) 1744, 1779
CviAII CATG 8 cut(s) 612, 659, 843, 1255, 1477, 1558, 1651, 1933
CviQI GTAC 6 cut(s) 346, 812, 991, 1526, 1566, 1733
DdeI CTNAG 4 cut(s) 504, 603, 1172, 1242
DraIII CACNNNGTG 2 cut(s) 1640, 1979
EaeI YGGCCR 3 cut(s) 64, 1194, 1935
Eam1104I CTCTTC 2 cut(s) 782, 1481
EarI CTCTTC 2 cut(s) 782, 1481
EciI GGCGGA 1 cut(s) 417
Eco130I CCWWGG 5 cut(s) 34, 178, 515, 1162, 1833
Eco31I GGTCTC 1 cut(s) 1875
Eco47I GGWCC 2 cut(s) 1211, 1829
Eco57I CTGAAG 1 cut(s) 144
Eco81I CCTNAGG 1 cut(s) 504
EcoNI CCTNNNNNAGG 1 cut(s) 1559
EcoRII CCWGG 3 cut(s) 1056, 1549, 1636
EcoT14I CCWWGG 5 cut(s) 34, 178, 515, 1162, 1833
EcoT22I ATGCAT 1 cut(s) 1207
ErhI CCWWGG 5 cut(s) 34, 178, 515, 1162, 1833
Esp3I CGTCTC 1 cut(s) 1729
FaeI CATG 8 cut(s) 615, 662, 846, 1258, 1480, 1561, 1654, 1936
FaqI GGGAC 5 cut(s) 563, 1342, 1787, 1815, 1930
FatI CATG 8 cut(s) 611, 658, 842, 1254, 1476, 1557, 1650, 1932
FauNDI CATATG 2 cut(s) 598, 1207
FbaI TGATCA 1 cut(s) 720
FokI GGATG 5 cut(s) 521, 1295, 1532, 1738, 1904
FspBI CTAG 1 cut(s) 60
GsaI CCCAGC 1 cut(s) 527
GsuI CTGGAG 2 cut(s) 1251, 1821
HaeIII GGCC 7 cut(s) 66, 1056, 1196, 1554, 1905, 1937, 1983
HapII CCGG 3 cut(s) 570, 1024, 1701
Hin1II CATG 8 cut(s) 615, 662, 846, 1258, 1480, 1561, 1654, 1936
HincII GTYRAC 2 cut(s) 666, 1416
HindII GTYRAC 2 cut(s) 666, 1416
HpaI GTTAAC 1 cut(s) 666
HpaII CCGG 3 cut(s) 570, 1024, 1701
HphI GGTGA 4 cut(s) 365, 715, 859, 1880
Hpy166II GTNNAC 3 cut(s) 666, 1416, 1733
Hpy188I TCNGA 8 cut(s) 583, 720, 959, 1215, 1271, 1509, 1810, 1843
Hpy188III TCNNGA 7 cut(s) 19, 171, 340, 503, 659, 1289, 1672
Hpy8I GTNNAC 3 cut(s) 666, 1416, 1733
Hpy99I CGWCG 2 cut(s) 1673, 1943
HpyAV CCTTC 3 cut(s) 238, 867, 874
HpyCH4III ACNGT 7 cut(s) 133, 811, 823, 915, 1405, 1876, 1979
HpyCH4IV ACGT 1 cut(s) 1589
HpyF10VI GCNNNNNNNGC 9 cut(s) 63, 200, 533, 1157, 1202, 1247, 1292, 1397, 1748
HpyF3I CTNAG 4 cut(s) 504, 603, 1172, 1242
HpySE526I ACGT 1 cut(s) 1589
Hsp92II CATG 8 cut(s) 615, 662, 846, 1258, 1480, 1561, 1654, 1936
Ksp22I TGATCA 1 cut(s) 720
KspAI GTTAAC 1 cut(s) 666
LmnI GCTCC 2 cut(s) 1031, 1802
LweI GCATC 6 cut(s) 450, 1192, 1273, 1282, 1600, 1760
MabI ACCWGGT 1 cut(s) 1636
MaeI CTAG 1 cut(s) 60
MaeII ACGT 1 cut(s) 1589
MaeIII GTNAC 4 cut(s) 838, 964, 1220, 1868
MboII GAAGA 8 cut(s) 199, 328, 399, 799, 852, 1498, 1532, 1957
MfeI CAATTG 1 cut(s) 1335
MflI RGATCY 6 cut(s) 404, 578, 1599, 1674, 1715, 1843
MhlI GDGCHC 1 cut(s) 1645
MlsI TGGCCA 2 cut(s) 66, 1196
MluCI AATT 6 cut(s) 636, 771, 877, 1335, 1395, 1535
MluNI TGGCCA 2 cut(s) 66, 1196
MlyI GAGTC 6 cut(s) 386, 614, 1411, 1687, 1831, 1952
MmeI TCCRAC 3 cut(s) 805, 1779, 1858
Mox20I TGGCCA 2 cut(s) 66, 1196
Mph1103I ATGCAT 1 cut(s) 1207
MroXI GAANNNNTTC 1 cut(s) 1629
MscI TGGCCA 2 cut(s) 66, 1196
MseI TTAA 4 cut(s) 665, 876, 900, 1596
MslI CAYNNNNRTG 2 cut(s) 72, 1481
Msp20I TGGCCA 2 cut(s) 66, 1196
MspA1I CMGCKG 3 cut(s) 527, 1313, 1859
MspI CCGG 3 cut(s) 570, 1024, 1701
MspR9I CCNGG 3 cut(s) 1058, 1551, 1638
MunI CAATTG 1 cut(s) 1335
Mva1269I GAATGC 1 cut(s) 1173
MvaI CCWGG 3 cut(s) 1058, 1551, 1638
MwoI GCNNNNNNNGC 9 cut(s) 63, 200, 533, 1157, 1202, 1247, 1292, 1397, 1748
NdeI CATATG 2 cut(s) 598, 1207
NlaIII CATG 8 cut(s) 615, 662, 846, 1258, 1480, 1561, 1654, 1936
NlaIV GGNNCC 6 cut(s) 521, 886, 1375, 1642, 1717, 1831
NmuCI GTSAC 2 cut(s) 1220, 1868
NsiI ATGCAT 1 cut(s) 1207
PagI TCATGA 1 cut(s) 658
PctI GAATGC 1 cut(s) 1173
PdmI GAANNNNTTC 1 cut(s) 1629
PfeI GAWTC 4 cut(s) 174, 1016, 1266, 1630
Ple19I CGATCG 1 cut(s) 837
PleI GAGTC 6 cut(s) 385, 613, 1411, 1687, 1830, 1951
PpsI GAGTC 6 cut(s) 385, 613, 1411, 1687, 1830, 1951
Ppu21I YACGTR 1 cut(s) 1590
Psp6I CCWGG 3 cut(s) 1056, 1549, 1636
PspFI CCCAGC 1 cut(s) 523
PspGI CCWGG 3 cut(s) 1056, 1549, 1636
PspN4I GGNNCC 6 cut(s) 521, 886, 1375, 1642, 1717, 1831
PspPI GGNCC 3 cut(s) 1211, 1829, 1904
PstI CTGCAG 1 cut(s) 1300
PstNI CAGNNNCTG 2 cut(s) 275, 1034
PsuI RGATCY 6 cut(s) 404, 578, 1599, 1674, 1715, 1843
PvuI CGATCG 1 cut(s) 837
PvuII CAGCTG 3 cut(s) 527, 1313, 1859
RsaI GTAC 6 cut(s) 347, 813, 992, 1527, 1567, 1734
RsaNI GTAC 6 cut(s) 346, 812, 991, 1526, 1566, 1733
RseI CAYNNNNRTG 2 cut(s) 72, 1481
SaqAI TTAA 4 cut(s) 665, 876, 900, 1596
Sau96I GGNCC 3 cut(s) 1211, 1829, 1904
SchI GAGTC 6 cut(s) 386, 614, 1411, 1687, 1831, 1952
ScrFI CCNGG 3 cut(s) 1058, 1551, 1638
SduI GDGCHC 1 cut(s) 1645
SexAI ACCWGGT 1 cut(s) 1636
SfaNI GCATC 6 cut(s) 450, 1192, 1273, 1282, 1600, 1760
SfcI CTRYAG 2 cut(s) 1152, 1296
SinI GGWCC 2 cut(s) 1211, 1829
SmiMI CAYNNNNRTG 2 cut(s) 72, 1481
SmlI CTYRAG 1 cut(s) 1889
SmoI CTYRAG 1 cut(s) 1889
SpeI ACTAGT 1 cut(s) 59
Sse9I AATT 6 cut(s) 636, 771, 877, 1335, 1395, 1535
SsiI CCGC 2 cut(s) 402, 1134
SspI AATATT 1 cut(s) 499
SspMI CTAG 1 cut(s) 60
StyD4I CCNGG 3 cut(s) 1056, 1549, 1636
StyI CCWWGG 5 cut(s) 34, 178, 515, 1162, 1833
TaaI ACNGT 7 cut(s) 133, 811, 823, 915, 1405, 1876, 1979
TaiI ACGT 1 cut(s) 1592
TaqI TCGA 1 cut(s) 1941
TasI AATT 6 cut(s) 636, 771, 877, 1335, 1395, 1535
TatI WGTACW 3 cut(s) 345, 811, 1732
TauI GCSGC 1 cut(s) 1136
TfiI GAWTC 4 cut(s) 174, 1016, 1266, 1630
Tru1I TTAA 4 cut(s) 665, 876, 900, 1596
Tru9I TTAA 4 cut(s) 665, 876, 900, 1596
TscAI CASTG 6 cut(s) 136, 168, 814, 1225, 1765, 1879
TseFI GTSAC 2 cut(s) 1220, 1868
Tsp45I GTSAC 2 cut(s) 1220, 1868
TspDTI ATGAA 2 cut(s) 200, 1499
TspGWI ACGGA 1 cut(s) 1295
TspRI CASTG 6 cut(s) 136, 168, 814, 1225, 1765, 1879
VpaK11BI GGWCC 2 cut(s) 1211, 1829
XagI CCTNNNNNAGG 1 cut(s) 1559
XcmI CCANNNNNNNNNTGG 1 cut(s) 217
XmnI GAANNNNTTC 1 cut(s) 1629
XspI CTAG 1 cut(s) 60
Zsp2I ATGCAT 1 cut(s) 1207
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.