Rmu_sc0017477.1_g000001

Pyrophosphate-energized vacuolar membrane proton

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0017477.1
OG0000897
Physical Location & Seq
Reverse (-)
2 .. 2388
2387 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0017477.1_g000001.1.cds

Sequence Viewer

Length: 1772 bp
atggtggcttttgcagtcttgatatttgttttccttggctccgtggagggatttagcactagtagccatacatgtgcttatgatgaaacaaagatatgcaaacctgctcttgcaaccgccatattcagtaccatttcctttttgcttggtgctataacttccgtggtttctggattccttggtatgaagattgccacgtatgcaaatgccagaactactttggaggcaagaaaaggtgttgggaaggcttttatagtagcatttagatcaggtgctgtcatgggatttctccttgctgcaaatggtttattagttttatacattgtgatccttgtcttcaagatgttctatggtgaggattggggtggtctttttgagtccataactgggtatggtcttggtggatcttccatggctctttttggcagagttggtggaggcatctataccaaagctgctgatgtcggtgctgatcttgttggcaaagttgaaaggaatattcctgaggatgacccaaggaaccctgctgtgattgctgataatgttggtgacaatgttggggatattgctggaatgggatctgatctctttggatcctatgctgagtcatcatgtgctgctcttgttgttgcttcaatttcttcatttgggatcaatcatgagttaactgcaatgttatatcctctcattgtcagttccattggtatcattgtttgtctgatcaccacccttttcgcaactgatttctttgagatcaagactgtaaaggaaattgagccagcattgaagaagcaactcattatttccacagtactgatgactgttggagttgcaattgttacttggatttctgttccttcttccttcaccatcttcaattttggaacacagaaggttgttaagaactggcagctgttcttgtgcgtgtgtgttgggttgtgggctgggcttatcatcggatttgtgacagagtattataccagtaatgcatacagccctgtgcaagatgttgctgattcgtgccggacaggagctgctactaatatcatatttggcctggcattgggatacaaatctgtcattattcctatttttgccattgctgttagtatttttgttagttttagctttgccgctatgtatggcattgctgtagccgcccttggaatgctgagcaccatagcaactggattggccattgatgcatatggtccgatcagtgacaatgctggtggcattgctgagatggcaggcatgagccacagaatcagagagagaactgatgctcttgatgctgcaggaaacaccacggctgctattggaaagggttttgcaattggctccgctgctcttgtgtcccttgctctctttggtgcctttgtgagccgtgctggaattgcaacagttgatgtgttgactccaaaagtgttcattggtttgatagtgggtgcaatgcttccctattggttctctgccatgacaatgaagagtgtgggaagtgctgctctaaagatggttgaagaagtacgcaggcaattcaacaccatccctggccttatggagggtactgctaaacctgactatgctacttgcgtcaagatctcgactgatgcttccatcaaggaaatgatccctcctggtgcccttgtcatgctaacacccctcattgtcggaatcttttttggtgtggagactctctctggtgtccttgctggatccctcgtctctggtgtacagatcgccatctctgcatccaacaccggtggcgcttg
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 map00190
Pfam Domains
Protein Families
H_PPase

Protein Analysis

590

Amino Acids

61.39

Weight (kDa)

5.43

Isoelectric Point (pI)

18.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 112
AccB1I GGYRCC 2 cut(s) 1373, 1640
AciI CCGC 4 cut(s) 117, 1134, 1158, 1344
AclWI GGATC 9 cut(s) 322, 412, 586, 588, 601, 659, 1624, 1710, 1723
AcoI YGGCCR 1 cut(s) 1194
AfaI GTAC 5 cut(s) 130, 813, 1527, 1567, 1734
AfiI CCNNNNNNNGG 3 cut(s) 387, 1063, 1561
AflIII ACRYGT 1 cut(s) 71
AgeI ACCGGT 1 cut(s) 1760
AgsI TTSAA 7 cut(s) 340, 491, 636, 787, 877, 1520, 1540
AhlI ACTAGT 1 cut(s) 59
AjnI CCWGG 3 cut(s) 1056, 1549, 1636
AluBI AGCT 4 cut(s) 455, 913, 1034, 1128
AluI AGCT 4 cut(s) 455, 913, 1034, 1128
Alw21I GWGCWC 1 cut(s) 1178
Alw26I GTCTC 2 cut(s) 1685, 1729
AlwI GGATC 9 cut(s) 322, 412, 586, 588, 601, 659, 1624, 1710, 1723
AlwNI CAGNNNCTG 2 cut(s) 275, 1034
AoxI GGCC 3 cut(s) 1054, 1194, 1552
ApeKI GCWGC 9 cut(s) 296, 455, 617, 910, 1034, 1295, 1313, 1346, 1502
AsiGI ACCGGT 1 cut(s) 1760
AspLEI GCGC 1 cut(s) 1769
AspS9I GGNCC 1 cut(s) 1211
AsuHPI GGTGA 4 cut(s) 365, 560, 715, 859
AvaII GGWCC 1 cut(s) 1211
AxyI CCTNAGG 1 cut(s) 504
BaeGI GKGCMC 1 cut(s) 1645
BalI TGGCCA 1 cut(s) 1196
BamHI GGATCC 2 cut(s) 593, 1715
BanI GGYRCC 2 cut(s) 1373, 1640
BbsI GAAGAC 1 cut(s) 328
Bbv12I GWGCWC 1 cut(s) 1178
BbvI GCAGC 9 cut(s) 283, 442, 604, 922, 1021, 1282, 1300, 1333, 1489
BccI CCATC 6 cut(s) 878, 1240, 1507, 1553, 1625, 1751
BceAI ACGGC 2 cut(s) 1326, 1371
BciT130I CCWGG 3 cut(s) 1058, 1551, 1638
BciVI GTATCC 1 cut(s) 1061
BclI TGATCA 1 cut(s) 720
BcoDI GTCTC 2 cut(s) 1685, 1729
BcuI ACTAGT 1 cut(s) 59
BfaI CTAG 1 cut(s) 60
BfmI CTRYAG 2 cut(s) 1152, 1296
BfoI RGCGCY 1 cut(s) 1770
BfuAI ACCTGC 1 cut(s) 112
BfuI GTATCC 1 cut(s) 1061
BglII AGATCT 1 cut(s) 1599
BlpI GCTNAGC 1 cut(s) 1172
BmcAI AGTACT 1 cut(s) 813
Bme1390I CCNGG 3 cut(s) 1058, 1551, 1638
Bme18I GGWCC 1 cut(s) 1211
BmgT120I GGNCC 1 cut(s) 1211
BmiI GGNNCC 7 cut(s) 40, 521, 595, 1342, 1375, 1642, 1717
BmrFI CCNGG 3 cut(s) 1058, 1551, 1638
BmrI ACTGGG 1 cut(s) 396
BmsI GCATC 6 cut(s) 450, 1192, 1273, 1282, 1600, 1760
BmuI ACTGGG 1 cut(s) 396
BpiI GAAGAC 1 cut(s) 328
BplI GAGNNNNNCTC 2 cut(s) 1682, 1714
Bpu1102I GCTNAGC 1 cut(s) 1172
BsaAI YACGTR 1 cut(s) 198
BsaBI GATNNNNATC 2 cut(s) 1072, 1742
BsaJI CCNNGG 9 cut(s) 34, 42, 162, 178, 411, 515, 1162, 1308, 1549
BsaWI WCCGGW 1 cut(s) 1760
Bsc4I CCNNNNNNNGG 3 cut(s) 387, 1063, 1561
Bse118I RCCGGY 1 cut(s) 1760
Bse1I ACTGG 4 cut(s) 391, 911, 981, 1192
Bse21I CCTNAGG 1 cut(s) 504
Bse3DI GCAATG 5 cut(s) 678, 1098, 1146, 1236, 1458
Bse8I GATNNNNATC 2 cut(s) 1072, 1742
BseBI CCWGG 3 cut(s) 1058, 1551, 1638
BseDI CCNNGG 9 cut(s) 34, 42, 162, 178, 411, 515, 1162, 1308, 1549
BseGI GGATG 3 cut(s) 514, 1545, 1751
BseJI GATNNNNATC 2 cut(s) 1072, 1742
BseLI CCNNNNNNNGG 3 cut(s) 387, 1063, 1561
BseMI GCAATG 5 cut(s) 678, 1098, 1146, 1236, 1458
BseMII CTCAG 4 cut(s) 495, 594, 1163, 1233
BseNI ACTGG 4 cut(s) 391, 911, 981, 1192
BseSI GKGCMC 1 cut(s) 1645
BseXI GCAGC 9 cut(s) 283, 442, 604, 922, 1021, 1282, 1300, 1333, 1489
BseYI CCCAGC 1 cut(s) 944
BshFI GGCC 3 cut(s) 1056, 1196, 1554
BshNI GGYRCC 2 cut(s) 1373, 1640
BshTI ACCGGT 1 cut(s) 1760
BsiHKAI GWGCWC 1 cut(s) 1178
BsiSI CCGG 2 cut(s) 1024, 1761
BslFI GGGAC 1 cut(s) 1342
BslI CCNNNNNNNGG 3 cut(s) 387, 1063, 1561
BsmAI GTCTC 2 cut(s) 1685, 1729
BsmBI CGTCTC 1 cut(s) 1729
BsmFI GGGAC 1 cut(s) 1342
BsmI GAATGC 1 cut(s) 1173
BsnI GGCC 3 cut(s) 1056, 1196, 1554
Bsp1286I GDGCHC 2 cut(s) 1178, 1645
Bsp1407I TGTACA 1 cut(s) 1732
Bsp1720I GCTNAGC 1 cut(s) 1172
Bsp19I CCATGG 1 cut(s) 411
BspACI CCGC 4 cut(s) 117, 1134, 1158, 1344
BspANI GGCC 3 cut(s) 1056, 1196, 1554
BspCNI CTCAG 4 cut(s) 496, 595, 1164, 1234
BspHI TCATGA 1 cut(s) 658
BspLI GGNNCC 7 cut(s) 40, 521, 595, 1342, 1375, 1642, 1717
BspMAI CTGCAG 1 cut(s) 1300
BspMI ACCTGC 1 cut(s) 112
BspPI GGATC 9 cut(s) 322, 412, 586, 588, 601, 659, 1624, 1710, 1723
BspT107I GGYRCC 2 cut(s) 1373, 1640
BsrDI GCAATG 5 cut(s) 678, 1098, 1146, 1236, 1458
BsrFI RCCGGY 1 cut(s) 1760
BsrGI TGTACA 1 cut(s) 1732
BsrI ACTGG 4 cut(s) 391, 911, 981, 1192
BssAI RCCGGY 1 cut(s) 1760
BssECI CCNNGG 9 cut(s) 34, 42, 162, 178, 411, 515, 1162, 1308, 1549
BssT1I CCWWGG 5 cut(s) 34, 178, 411, 515, 1162
Bst2UI CCWGG 3 cut(s) 1058, 1551, 1638
Bst4CI ACNGT 4 cut(s) 763, 811, 823, 1405
Bst6I CTCTTC 1 cut(s) 1481
BstAUI TGTACA 1 cut(s) 1732
BstBAI YACGTR 1 cut(s) 198
BstC8I GCNNGC 3 cut(s) 780, 1252, 1532
BstDEI CTNAG 4 cut(s) 504, 603, 1172, 1242
BstDSI CCRYGG 4 cut(s) 42, 162, 411, 1308
BstENI CCTNNNNNAGG 1 cut(s) 1559
BstF5I GGATG 3 cut(s) 514, 1545, 1751
BstH2I RGCGCY 1 cut(s) 1770
BstHHI GCGC 1 cut(s) 1769
BstMAI GTCTC 2 cut(s) 1685, 1729
BstMWI GCNNNNNNNGC 9 cut(s) 63, 200, 533, 1157, 1202, 1247, 1292, 1397, 1748
BstNI CCWGG 3 cut(s) 1058, 1551, 1638
BstNSI RCATGY 1 cut(s) 75
BstSCI CCNGG 3 cut(s) 1056, 1549, 1636
BstSFI CTRYAG 2 cut(s) 1152, 1296
BstSLI GKGCMC 1 cut(s) 1645
BstV1I GCAGC 9 cut(s) 283, 442, 604, 922, 1021, 1282, 1300, 1333, 1489
BstV2I GAAGAC 1 cut(s) 328
BstX2I RGATCY 5 cut(s) 404, 578, 593, 1599, 1715
BstYI RGATCY 5 cut(s) 404, 578, 593, 1599, 1715
Bsu36I CCTNAGG 1 cut(s) 504
BsuI GTATCC 1 cut(s) 1061
BsuRI GGCC 3 cut(s) 1056, 1196, 1554
BtgI CCRYGG 4 cut(s) 42, 162, 411, 1308
BtsCI GGATG 3 cut(s) 514, 1545, 1751
BtsIMutI CAGTG 1 cut(s) 1225
BveI ACCTGC 1 cut(s) 112
Cac8I GCNNGC 3 cut(s) 780, 1252, 1532
CaiI CAGNNNCTG 2 cut(s) 275, 1034
CciI TCATGA 1 cut(s) 658
CfoI GCGC 1 cut(s) 1769
Cfr10I RCCGGY 1 cut(s) 1760
Cfr13I GGNCC 1 cut(s) 1211
CseI GACGC 1 cut(s) 1582
Csp6I GTAC 5 cut(s) 129, 812, 1526, 1566, 1733
CspAI ACCGGT 1 cut(s) 1760
CspCI CAANNNNNGTGG 3 cut(s) 1213, 1248, 1744
CviAII CATG 8 cut(s) 72, 280, 412, 612, 659, 1255, 1477, 1651
CviQI GTAC 5 cut(s) 129, 812, 1526, 1566, 1733
DdeI CTNAG 4 cut(s) 504, 603, 1172, 1242
EaeI YGGCCR 1 cut(s) 1194
Eam1104I CTCTTC 1 cut(s) 1481
EarI CTCTTC 1 cut(s) 1481
Eco130I CCWWGG 5 cut(s) 34, 178, 411, 515, 1162
Eco47I GGWCC 1 cut(s) 1211
Eco81I CCTNAGG 1 cut(s) 504
EcoNI CCTNNNNNAGG 1 cut(s) 1559
EcoRII CCWGG 3 cut(s) 1056, 1549, 1636
EcoT14I CCWWGG 5 cut(s) 34, 178, 411, 515, 1162
EcoT22I ATGCAT 2 cut(s) 991, 1207
ErhI CCWWGG 5 cut(s) 34, 178, 411, 515, 1162
Esp3I CGTCTC 1 cut(s) 1729
FaeI CATG 8 cut(s) 75, 283, 415, 615, 662, 1258, 1480, 1654
FaqI GGGAC 1 cut(s) 1342
FatI CATG 8 cut(s) 71, 279, 411, 611, 658, 1254, 1476, 1650
FauNDI CATATG 1 cut(s) 1207
FbaI TGATCA 1 cut(s) 720
FokI GGATG 3 cut(s) 521, 1532, 1738
FspBI CTAG 1 cut(s) 60
GlaI GCGC 1 cut(s) 1768
GsaI CCCAGC 1 cut(s) 948
HaeII RGCGCY 1 cut(s) 1770
HaeIII GGCC 3 cut(s) 1056, 1196, 1554
HapII CCGG 2 cut(s) 1024, 1761
HgaI GACGC 1 cut(s) 1582
HhaI GCGC 1 cut(s) 1769
Hin1II CATG 8 cut(s) 75, 283, 415, 615, 662, 1258, 1480, 1654
Hin6I GCGC 1 cut(s) 1767
HinP1I GCGC 1 cut(s) 1767
HincII GTYRAC 2 cut(s) 666, 1416
HindII GTYRAC 2 cut(s) 666, 1416
HinfI GANTC 8 cut(s) 174, 377, 605, 1016, 1266, 1417, 1674, 1693
HpaI GTTAAC 1 cut(s) 666
HpaII CCGG 2 cut(s) 1024, 1761
HphI GGTGA 4 cut(s) 365, 560, 715, 859
Hpy166II GTNNAC 3 cut(s) 666, 1416, 1733
Hpy188I TCNGA 6 cut(s) 583, 720, 959, 1215, 1271, 1673
Hpy188III TCNNGA 9 cut(s) 19, 171, 340, 503, 659, 757, 1289, 1597, 1603
Hpy8I GTNNAC 3 cut(s) 666, 1416, 1733
HpyAV CCTTC 4 cut(s) 238, 867, 874, 886
HpyCH4III ACNGT 4 cut(s) 763, 811, 823, 1405
HpyCH4IV ACGT 1 cut(s) 197
HpyF10VI GCNNNNNNNGC 9 cut(s) 63, 200, 533, 1157, 1202, 1247, 1292, 1397, 1748
HpyF3I CTNAG 4 cut(s) 504, 603, 1172, 1242
HpySE526I ACGT 1 cut(s) 197
Hsp92II CATG 8 cut(s) 75, 283, 415, 615, 662, 1258, 1480, 1654
HspAI GCGC 1 cut(s) 1767
Ksp22I TGATCA 1 cut(s) 720
KspAI GTTAAC 1 cut(s) 666
LmnI GCTCC 3 cut(s) 44, 1031, 1346
Lsp1109I GCAGC 9 cut(s) 283, 442, 604, 922, 1021, 1282, 1300, 1333, 1489
LweI GCATC 6 cut(s) 450, 1192, 1273, 1282, 1600, 1760
MaeI CTAG 1 cut(s) 60
MaeII ACGT 1 cut(s) 197
MaeIII GTNAC 4 cut(s) 548, 838, 964, 1220
MboII GAAGA 9 cut(s) 199, 328, 399, 633, 799, 852, 865, 1498, 1532
MfeI CAATTG 2 cut(s) 834, 1335
MflI RGATCY 5 cut(s) 404, 578, 593, 1599, 1715
MhlI GDGCHC 2 cut(s) 1178, 1645
MlsI TGGCCA 1 cut(s) 1196
MluCI AATT 7 cut(s) 636, 771, 834, 877, 1335, 1395, 1535
MluNI TGGCCA 1 cut(s) 1196
MlyI GAGTC 4 cut(s) 386, 614, 1411, 1687
MmeI TCCRAC 2 cut(s) 805, 1651
Mox20I TGGCCA 1 cut(s) 1196
Mph1103I ATGCAT 2 cut(s) 991, 1207
MscI TGGCCA 1 cut(s) 1196
MseI TTAA 2 cut(s) 665, 900
MslI CAYNNNNRTG 2 cut(s) 72, 1481
Msp20I TGGCCA 1 cut(s) 1196
MspA1I CMGCKG 2 cut(s) 913, 1346
MspI CCGG 2 cut(s) 1024, 1761
MspR9I CCNGG 3 cut(s) 1058, 1551, 1638
MunI CAATTG 2 cut(s) 834, 1335
Mva1269I GAATGC 1 cut(s) 1173
MvaI CCWGG 3 cut(s) 1058, 1551, 1638
MwoI GCNNNNNNNGC 9 cut(s) 63, 200, 533, 1157, 1202, 1247, 1292, 1397, 1748
NcoI CCATGG 1 cut(s) 411
NdeI CATATG 1 cut(s) 1207
NlaIII CATG 8 cut(s) 75, 283, 415, 615, 662, 1258, 1480, 1654
NlaIV GGNNCC 7 cut(s) 40, 521, 595, 1342, 1375, 1642, 1717
NmuCI GTSAC 3 cut(s) 548, 964, 1220
NsiI ATGCAT 2 cut(s) 991, 1207
NspI RCATGY 1 cut(s) 75
PagI TCATGA 1 cut(s) 658
PciI ACATGT 1 cut(s) 71
PctI GAATGC 1 cut(s) 1173
PfeI GAWTC 4 cut(s) 174, 1016, 1266, 1674
PinAI ACCGGT 1 cut(s) 1760
PleI GAGTC 4 cut(s) 385, 613, 1411, 1687
PpsI GAGTC 4 cut(s) 385, 613, 1411, 1687
Ppu21I YACGTR 1 cut(s) 198
PscI ACATGT 1 cut(s) 71
Psp6I CCWGG 3 cut(s) 1056, 1549, 1636
PspFI CCCAGC 1 cut(s) 944
PspGI CCWGG 3 cut(s) 1056, 1549, 1636
PspN4I GGNNCC 7 cut(s) 40, 521, 595, 1342, 1375, 1642, 1717
PspPI GGNCC 1 cut(s) 1211
PstI CTGCAG 1 cut(s) 1300
PstNI CAGNNNCTG 2 cut(s) 275, 1034
PsuI RGATCY 5 cut(s) 404, 578, 593, 1599, 1715
PvuII CAGCTG 1 cut(s) 913
RsaI GTAC 5 cut(s) 130, 813, 1527, 1567, 1734
RsaNI GTAC 5 cut(s) 129, 812, 1526, 1566, 1733
RseI CAYNNNNRTG 2 cut(s) 72, 1481
SaqAI TTAA 2 cut(s) 665, 900
Sau96I GGNCC 1 cut(s) 1211
ScaI AGTACT 1 cut(s) 813
SchI GAGTC 4 cut(s) 386, 614, 1411, 1687
ScrFI CCNGG 3 cut(s) 1058, 1551, 1638
SduI GDGCHC 2 cut(s) 1178, 1645
SfaNI GCATC 6 cut(s) 450, 1192, 1273, 1282, 1600, 1760
SfcI CTRYAG 2 cut(s) 1152, 1296
SgrAI CRCCGGYG 1 cut(s) 1760
SinI GGWCC 1 cut(s) 1211
SmiMI CAYNNNNRTG 2 cut(s) 72, 1481
SpeI ACTAGT 1 cut(s) 59
Sse9I AATT 7 cut(s) 636, 771, 834, 877, 1335, 1395, 1535
SsiI CCGC 4 cut(s) 117, 1134, 1158, 1344
SspI AATATT 1 cut(s) 499
SspMI CTAG 1 cut(s) 60
StyD4I CCNGG 3 cut(s) 1056, 1549, 1636
StyI CCWWGG 5 cut(s) 34, 178, 411, 515, 1162
TaaI ACNGT 4 cut(s) 763, 811, 823, 1405
TaiI ACGT 1 cut(s) 200
TaqI TCGA 1 cut(s) 1604
TasI AATT 7 cut(s) 636, 771, 834, 877, 1335, 1395, 1535
TatI WGTACW 2 cut(s) 811, 1732
TauI GCSGC 2 cut(s) 1136, 1160
TfiI GAWTC 4 cut(s) 174, 1016, 1266, 1674
Tru1I TTAA 2 cut(s) 665, 900
Tru9I TTAA 2 cut(s) 665, 900
TscAI CASTG 1 cut(s) 1225
TseFI GTSAC 3 cut(s) 548, 964, 1220
TseI GCWGC 9 cut(s) 296, 455, 617, 910, 1034, 1295, 1313, 1346, 1502
Tsp45I GTSAC 3 cut(s) 548, 964, 1220
TspDTI ATGAA 5 cut(s) 99, 200, 633, 1420, 1499
TspGWI ACGGA 2 cut(s) 31, 151
TspRI CASTG 1 cut(s) 1225
VpaK11BI GGWCC 1 cut(s) 1211
XagI CCTNNNNNAGG 1 cut(s) 1559
XceI RCATGY 1 cut(s) 75
XcmI CCANNNNNNNNNTGG 1 cut(s) 217
XspI CTAG 1 cut(s) 60
ZrmI AGTACT 1 cut(s) 813
Zsp2I ATGCAT 2 cut(s) 991, 1207
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.