Rh2DG027300

Pyrophosphate-energized vacuolar membrane proton

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0000897
Physical Location & Seq
Reverse (-)
1639840 .. 1644966
5127 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG027300.1

Sequence Viewer

Length: 2298 bp
ATGGGGGCAACTATTCTCCCAGATCTCGGGGCGGAGATTCTGATTCCGTTCTGCGCGGTGGTCGGAATCGTGTTCTCGCTGCTCCAGTGGGTGTACGTGTCACGCGTCCAGCTCTCCCCGGCGAGGGACTCCAACTCTAACTCCGCCGCAAAAAATGGTTACAACGATTACCTGATTGAGGAGGAAGAGGGTGTTAACGACACCAACGTTGTTCAGAAGTGCGCCGAAATCCAGAACGCCATCTCTGAAGGAGCAACATCATTCCTTTTCACGGAATATAAGTATGTAGGTGCTTTTATGGTGGCTTTTGCAGTCTTGATATTTGTTTTCCTTGGCTCCGTGGAGGGATTTAGCACTAGTAGCCATACATGTGCTTATGATGAAACAAAGATATGCAAACCTGCTCTTGCAACTGCCATATTCAGTACCATTTCCTTTTTGCTTGGTGCTATAACTTCAGTGGTGTCTGGATTCCTTGGTATGAAGATTGCCACGTATGCAAATGCCAGAACTACTTTGGAGGCAAGAAAAGGTGTTGGGAAGGCTTTTATAGTAGCATTTAGATCAGGTGCTGTCATGGGATTTCTCCTTGCTGCAAATGGTTTATTAGTTTTATACATTGTGATCCTTGTCTTCAAGATGTTCTATGGTGAGGATTGGGGTGGTCTTTTTGAGTCCATAACTGGGTATGGTCTTGGTGGATCTTCCATGGCTCTTTTTGGCAGAGTTGGTGGAGGCATCTATACCAAAGCTGCTGATGTCGGTGCTGATCTTGTTGGCAAAGTTGAAAGGAATATTCCTGAGGATGACCCAAGGAACCCTGCTGTGATTGCTGATAATGTTGGTGACAATGTTGGGGATATTGCTGGAATGGGATCTGATCTCTTTGGATCCTATGCTGAGTCATCATGTGCTGCTCTTGTTGTTGCTTCAATTTCTTCATTTGGGATCAATCATGAGTTAACTGCAATGTTATATCCTCTCATTGTCAGTTCCATTGGTATCATTGTTTGTCTGATCACCACCCTTTTCGCAACTGATTTCTTTGAGATCAAGACTGTAAAGGAAATTGAGCCAGCATTGAAGAAGCAACTCATTATTTCCACAGTACTGATGACTGTTGGAGTTGCAATTGTTACTTGGATTTCTGTTCCTTCTTCCTTCACCATCTTCAATTTTGGAACACAGAAGGTTGTTAAGAACTGGCAGCTGTTCTTGTGCGTGTGTGTTGGGTTGTGGGCTGGGCTTATCATCGGATTTGTGACAGAGTATTATACCAGTAATGCATACAGCCCTGTGCAAGATGTTGCTGATTCGTGCCGGACAGGAGCTGCTACTAATATCATATTTGGCCTGGCATTGGGATACAAATCTGTCATTATTCCTATTTTTGCCATTGCTGTTAGTATTTTTGTTAGTTTTAGCTTTGCCGCTATGTATGGCATTGCTGTAGCCGCCCTTGGAATGCTGAGCACCATAGCAACTGGATTGGCCATTGATGCATATGGTCCGATCAGTGACAATGCTGGTGGCATTGCTGAGATGGCAGGCATGAGCCACAGAATCAGAGAGAGAACTGATGCTCTTGATGCTGCAGGAAACACCACGGCTGCTATTGGAAAGGGTTTTGCAATTGGCTCCGCTGCTCTTGTGTCCCTTGCTCTCTTTGGTGCCTTTGTGAGCCGTGCTGGAATTGCAACAGTTGATGTGTTGACTCCAAAAGTGTTCATTGGTTTGATAGTGGGTGCAATGCTTCCCTATTGGTTCTCTGCCATGACAATGAAGAGTGTGGGAAGTGCTGCTCTAAAGATGGTTGAAGAAGTACGCAGGCAATTCAACACCATCCCTGGCCTTATGGAGGGTACTGCTAAACCTGACTATGCTACTTGCGTCAAGATCTCGACTGATGCTTCCATCAAGGAAATGATCCCTCCTGGTGCCCTTGTCATGCTAACACCCCTCATTGTCGGAATCTTTTTTGGTGTGGAGACTCTCTCTGGTGTCCTTGCTGGATCCCTCGTCTCTGGTGTACAGATCGCCATCTCTGCATCCAACACCGGTGGCGCTTGGGACAATGCCAAGAAGTATATTGAGGCCGGGGCTTCAGAACATGCAAGGACTCTTGGTCCCAAGGGATCAGATCCACACAAAGCAGCTGTCATTGGTGACACCGTCGGAGACCCTCTCAAGGACACATCCGGCCCATCTCTCAACATCCTCATCAAGCTGATGGCTGTCGAGTCCCTAGTGTTTGCACCTTTCTTCGCCACACATGGCGGTCTCCTTTTCAAGCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 map00190
Pfam Domains
Protein Families
H_PPase

Protein Analysis

765

Amino Acids

80.18

Weight (kDa)

5.18

Isoelectric Point (pI)

22.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
H_PPase PF03030 63 - 751 5.9e-258 Inorganic H+ pyrophosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 409
AccB1I GGYRCC 2 cut(s) 1670, 1937
AccII CGCG 2 cut(s) 56, 105
AciI CCGC 8 cut(s) 32, 56, 144, 147, 1431, 1455, 1641, 2277
AclI AACGTT 1 cut(s) 207
AcoI YGGCCR 1 cut(s) 1491
AcuI CTGAAG 3 cut(s) 267, 441, 2088
AfaI GTAC 6 cut(s) 95, 427, 1110, 1824, 1864, 2031
AfiI CCNNNNNNNGG 9 cut(s) 26, 123, 124, 178, 271, 684, 1360, 1858, 2188
AflIII ACRYGT 3 cut(s) 96, 103, 368
AgeI ACCGGT 1 cut(s) 2057
AgsI TTSAA 8 cut(s) 637, 788, 933, 1084, 1174, 1817, 1837, 2290
AhdI GACNNNNNGTC 1 cut(s) 2124
AhlI ACTAGT 1 cut(s) 356
AjnI CCWGG 3 cut(s) 1353, 1846, 1933
AluBI AGCT 8 cut(s) 112, 752, 1210, 1331, 1425, 2156, 2227, 2293
AluI AGCT 8 cut(s) 112, 752, 1210, 1331, 1425, 2156, 2227, 2293
Alw21I GWGCWC 1 cut(s) 1475
Alw26I GTCTC 4 cut(s) 1982, 2026, 2172, 2285
AlwNI CAGNNNCTG 2 cut(s) 572, 1331
Ama87I CYCGRG 1 cut(s) 26
AoxI GGCC 5 cut(s) 1351, 1491, 1849, 2094, 2200
ArsI GACNNNNNNTTYG 2 cut(s) 2142, 2174
AsiGI ACCGGT 1 cut(s) 2057
AspLEI GCGC 3 cut(s) 56, 224, 2066
AspS9I GGNCC 3 cut(s) 1508, 2126, 2201
AsuC2I CCSGG 2 cut(s) 119, 2098
AsuHPI GGTGA 5 cut(s) 662, 857, 1012, 1156, 2177
AvaI CYCGRG 1 cut(s) 26
AvaII GGWCC 2 cut(s) 1508, 2126
AxyI CCTNAGG 1 cut(s) 801
BaeGI GKGCMC 1 cut(s) 1942
BalI TGGCCA 1 cut(s) 1493
BamHI GGATCC 2 cut(s) 890, 2012
BanI GGYRCC 2 cut(s) 1670, 1937
BbsI GAAGAC 1 cut(s) 625
Bbv12I GWGCWC 1 cut(s) 1475
BccI CCATC 9 cut(s) 248, 1175, 1537, 1804, 1850, 1922, 2048, 2212, 2224
BceAI ACGGC 2 cut(s) 1623, 1668
BciT130I CCWGG 3 cut(s) 1355, 1848, 1935
BciVI GTATCC 1 cut(s) 1358
BclI TGATCA 1 cut(s) 1017
BcnI CCSGG 2 cut(s) 119, 2098
BcoDI GTCTC 4 cut(s) 1982, 2026, 2172, 2285
BcuI ACTAGT 1 cut(s) 356
BfaI CTAG 2 cut(s) 357, 2246
BfmI CTRYAG 2 cut(s) 1449, 1593
BfoI RGCGCY 1 cut(s) 2067
BfuAI ACCTGC 1 cut(s) 409
BfuI GTATCC 1 cut(s) 1358
BglII AGATCT 2 cut(s) 22, 1896
BlpI GCTNAGC 1 cut(s) 1469
BmcAI AGTACT 1 cut(s) 1110
Bme1390I CCNGG 5 cut(s) 119, 1355, 1848, 1935, 2098
Bme18I GGWCC 2 cut(s) 1508, 2126
BmeRI GACNNNNNGTC 1 cut(s) 2124
BmeT110I CYCGRG 1 cut(s) 26
BmgT120I GGNCC 3 cut(s) 1508, 2126, 2201
BmiI GGNNCC 8 cut(s) 337, 818, 892, 1639, 1672, 1939, 2014, 2128
BmrFI CCNGG 5 cut(s) 119, 1355, 1848, 1935, 2098
BmrI ACTGGG 1 cut(s) 693
BmsI GCATC 6 cut(s) 747, 1489, 1570, 1579, 1897, 2057
BmuI ACTGGG 1 cut(s) 693
BpiI GAAGAC 1 cut(s) 625
BplI GAGNNNNNCTC 4 cut(s) 1979, 2011, 2169, 2201
BpmI CTGGAG 1 cut(s) 68
Bpu1102I GCTNAGC 1 cut(s) 1469
BpuEI CTTGAG 1 cut(s) 2171
BpuMI CCSGG 2 cut(s) 119, 2098
BsaAI YACGTR 2 cut(s) 97, 495
BsaBI GATNNNNATC 2 cut(s) 1369, 2039
BsaI GGTCTC 2 cut(s) 2172, 2285
BsaWI WCCGGW 1 cut(s) 2057
BsaXI ACNNNNNCTCC 2 cut(s) 125, 155
Bsc4I CCNNNNNNNGG 9 cut(s) 26, 123, 124, 178, 271, 684, 1360, 1858, 2188
Bse118I RCCGGY 1 cut(s) 2057
Bse1I ACTGG 5 cut(s) 85, 688, 1208, 1278, 1489
Bse21I CCTNAGG 1 cut(s) 801
Bse3DI GCAATG 5 cut(s) 975, 1395, 1443, 1533, 1755
Bse8I GATNNNNATC 2 cut(s) 1369, 2039
BseBI CCWGG 3 cut(s) 1355, 1848, 1935
BseGI GGATG 5 cut(s) 811, 1842, 2048, 2195, 2214
BseJI GATNNNNATC 2 cut(s) 1369, 2039
BseLI CCNNNNNNNGG 9 cut(s) 26, 123, 124, 178, 271, 684, 1360, 1858, 2188
BseMI GCAATG 5 cut(s) 975, 1395, 1443, 1533, 1755
BseMII CTCAG 4 cut(s) 792, 891, 1460, 1530
BseNI ACTGG 5 cut(s) 85, 688, 1208, 1278, 1489
BseRI GAGGAG 1 cut(s) 194
BseSI GKGCMC 1 cut(s) 1942
BseYI CCCAGC 1 cut(s) 1241
Bsh1236I CGCG 2 cut(s) 56, 105
BshFI GGCC 5 cut(s) 1353, 1493, 1851, 2096, 2202
BshNI GGYRCC 2 cut(s) 1670, 1937
BshTI ACCGGT 1 cut(s) 2057
BsiHKAI GWGCWC 1 cut(s) 1475
BsiHKCI CYCGRG 1 cut(s) 26
BsiSI CCGG 5 cut(s) 119, 1321, 2058, 2097, 2199
BslFI GGGAC 5 cut(s) 140, 1639, 2084, 2112, 2227
BslI CCNNNNNNNGG 9 cut(s) 26, 123, 124, 178, 271, 684, 1360, 1858, 2188
BsmAI GTCTC 4 cut(s) 1982, 2026, 2172, 2285
BsmBI CGTCTC 1 cut(s) 2026
BsmFI GGGAC 5 cut(s) 140, 1639, 2084, 2112, 2227
BsmI GAATGC 1 cut(s) 1470
BsnI GGCC 5 cut(s) 1353, 1493, 1851, 2096, 2202
Bso31I GGTCTC 2 cut(s) 2172, 2285
BsoBI CYCGRG 1 cut(s) 26
Bsp1286I GDGCHC 2 cut(s) 1475, 1942
Bsp1407I TGTACA 1 cut(s) 2029
Bsp1720I GCTNAGC 1 cut(s) 1469
Bsp19I CCATGG 1 cut(s) 708
BspACI CCGC 8 cut(s) 32, 56, 144, 147, 1431, 1455, 1641, 2277
BspANI GGCC 5 cut(s) 1353, 1493, 1851, 2096, 2202
BspCNI CTCAG 4 cut(s) 793, 892, 1461, 1531
BspFNI CGCG 2 cut(s) 56, 105
BspHI TCATGA 1 cut(s) 955
BspLI GGNNCC 8 cut(s) 337, 818, 892, 1639, 1672, 1939, 2014, 2128
BspMAI CTGCAG 1 cut(s) 1597
BspMI ACCTGC 1 cut(s) 409
BspT107I GGYRCC 2 cut(s) 1670, 1937
BspTNI GGTCTC 2 cut(s) 2172, 2285
BsrDI GCAATG 5 cut(s) 975, 1395, 1443, 1533, 1755
BsrFI RCCGGY 1 cut(s) 2057
BsrGI TGTACA 1 cut(s) 2029
BsrI ACTGG 5 cut(s) 85, 688, 1208, 1278, 1489
BssAI RCCGGY 1 cut(s) 2057
BssT1I CCWWGG 6 cut(s) 331, 475, 708, 812, 1459, 2130
Bst2UI CCWGG 3 cut(s) 1355, 1848, 1935
Bst4CI ACNGT 5 cut(s) 1060, 1108, 1120, 1702, 2173
Bst6I CTCTTC 2 cut(s) 180, 1778
BstAUI TGTACA 1 cut(s) 2029
BstBAI YACGTR 2 cut(s) 97, 495
BstC8I GCNNGC 3 cut(s) 1077, 1549, 1829
BstDEI CTNAG 4 cut(s) 801, 900, 1469, 1539
BstDSI CCRYGG 3 cut(s) 339, 708, 1605
BstENI CCTNNNNNAGG 2 cut(s) 176, 1856
BstF5I GGATG 5 cut(s) 811, 1842, 2048, 2195, 2214
BstFNI CGCG 2 cut(s) 56, 105
BstH2I RGCGCY 1 cut(s) 2067
BstHHI GCGC 3 cut(s) 56, 224, 2066
BstMAI GTCTC 4 cut(s) 1982, 2026, 2172, 2285
BstMWI GCNNNNNNNGC 9 cut(s) 360, 497, 830, 1454, 1499, 1544, 1589, 1694, 2045
BstNI CCWGG 3 cut(s) 1355, 1848, 1935
BstNSI RCATGY 2 cut(s) 372, 2114
BstSCI CCNGG 5 cut(s) 117, 1353, 1846, 1933, 2096
BstSFI CTRYAG 2 cut(s) 1449, 1593
BstSLI GKGCMC 1 cut(s) 1942
BstUI CGCG 2 cut(s) 56, 105
BstV2I GAAGAC 1 cut(s) 625
BstX2I RGATCY 7 cut(s) 22, 701, 875, 890, 1896, 2012, 2140
BstYI RGATCY 7 cut(s) 22, 701, 875, 890, 1896, 2012, 2140
Bsu36I CCTNAGG 1 cut(s) 801
BsuI GTATCC 1 cut(s) 1358
BsuRI GGCC 5 cut(s) 1353, 1493, 1851, 2096, 2202
BtgI CCRYGG 3 cut(s) 339, 708, 1605
BtsCI GGATG 5 cut(s) 811, 1842, 2048, 2195, 2214
BtsIMutI CAGTG 3 cut(s) 92, 465, 1522
BveI ACCTGC 1 cut(s) 409
Cac8I GCNNGC 3 cut(s) 1077, 1549, 1829
CaiI CAGNNNCTG 2 cut(s) 572, 1331
CciI TCATGA 1 cut(s) 955
CfoI GCGC 3 cut(s) 56, 224, 2066
Cfr10I RCCGGY 1 cut(s) 2057
Cfr13I GGNCC 3 cut(s) 1508, 2126, 2201
CseI GACGC 2 cut(s) 94, 1879
Csp6I GTAC 6 cut(s) 94, 426, 1109, 1823, 1863, 2030
CspAI ACCGGT 1 cut(s) 2057
CspCI CAANNNNNGTGG 4 cut(s) 1510, 1545, 2041, 2076
CviQI GTAC 6 cut(s) 94, 426, 1109, 1823, 1863, 2030
DdeI CTNAG 4 cut(s) 801, 900, 1469, 1539
DriI GACNNNNNGTC 1 cut(s) 2124
EaeI YGGCCR 1 cut(s) 1491
Eam1104I CTCTTC 2 cut(s) 180, 1778
Eam1105I GACNNNNNGTC 1 cut(s) 2124
EarI CTCTTC 2 cut(s) 180, 1778
EciI GGCGGA 2 cut(s) 47, 133
Eco130I CCWWGG 6 cut(s) 331, 475, 708, 812, 1459, 2130
Eco31I GGTCTC 2 cut(s) 2172, 2285
Eco47I GGWCC 2 cut(s) 1508, 2126
Eco57I CTGAAG 3 cut(s) 267, 441, 2088
Eco81I CCTNAGG 1 cut(s) 801
Eco88I CYCGRG 1 cut(s) 26
EcoNI CCTNNNNNAGG 2 cut(s) 176, 1856
EcoRII CCWGG 3 cut(s) 1353, 1846, 1933
EcoT14I CCWWGG 6 cut(s) 331, 475, 708, 812, 1459, 2130
EcoT22I ATGCAT 2 cut(s) 1288, 1504
ErhI CCWWGG 6 cut(s) 331, 475, 708, 812, 1459, 2130
Esp3I CGTCTC 1 cut(s) 2026
FaqI GGGAC 5 cut(s) 140, 1639, 2084, 2112, 2227
FauNDI CATATG 1 cut(s) 1504
FbaI TGATCA 1 cut(s) 1017
FokI GGATG 5 cut(s) 818, 1829, 2035, 2182, 2201
FspBI CTAG 2 cut(s) 357, 2246
GlaI GCGC 3 cut(s) 55, 223, 2065
GsaI CCCAGC 1 cut(s) 1245
GsuI CTGGAG 1 cut(s) 68
HaeII RGCGCY 1 cut(s) 2067
HaeIII GGCC 5 cut(s) 1353, 1493, 1851, 2096, 2202
HapII CCGG 5 cut(s) 119, 1321, 2058, 2097, 2199
HgaI GACGC 2 cut(s) 94, 1879
HhaI GCGC 3 cut(s) 56, 224, 2066
Hin6I GCGC 3 cut(s) 54, 222, 2064
HinP1I GCGC 3 cut(s) 54, 222, 2064
HincII GTYRAC 3 cut(s) 196, 963, 1713
HindII GTYRAC 3 cut(s) 196, 963, 1713
HindIII AAGCTT 1 cut(s) 2291
HpaI GTTAAC 2 cut(s) 196, 963
HpaII CCGG 5 cut(s) 119, 1321, 2058, 2097, 2199
HphI GGTGA 5 cut(s) 662, 857, 1012, 1156, 2177
Hpy166II GTNNAC 5 cut(s) 94, 196, 963, 1713, 2030
Hpy8I GTNNAC 5 cut(s) 94, 196, 963, 1713, 2030
Hpy99I CGWCG 1 cut(s) 2177
HpyAV CCTTC 5 cut(s) 242, 535, 1164, 1171, 1183
HpyCH4III ACNGT 5 cut(s) 1060, 1108, 1120, 1702, 2173
HpyCH4IV ACGT 3 cut(s) 96, 207, 494
HpyF10VI GCNNNNNNNGC 9 cut(s) 360, 497, 830, 1454, 1499, 1544, 1589, 1694, 2045
HpyF3I CTNAG 4 cut(s) 801, 900, 1469, 1539
HpySE526I ACGT 3 cut(s) 96, 207, 494
HspAI GCGC 3 cut(s) 54, 222, 2064
Ksp22I TGATCA 1 cut(s) 1017
KspAI GTTAAC 2 cut(s) 196, 963
LmnI GCTCC 5 cut(s) 87, 251, 341, 1328, 1643
LweI GCATC 6 cut(s) 747, 1489, 1570, 1579, 1897, 2057
MaeI CTAG 2 cut(s) 357, 2246
MaeII ACGT 3 cut(s) 96, 207, 494
MaeIII GTNAC 7 cut(s) 99, 158, 845, 1135, 1261, 1517, 2165
MfeI CAATTG 2 cut(s) 1131, 1632
MflI RGATCY 7 cut(s) 22, 701, 875, 890, 1896, 2012, 2140
MhlI GDGCHC 2 cut(s) 1475, 1942
MlsI TGGCCA 1 cut(s) 1493
MluCI AATT 7 cut(s) 933, 1068, 1131, 1174, 1632, 1692, 1832
MluI ACGCGT 1 cut(s) 103
MluNI TGGCCA 1 cut(s) 1493
MlyI GAGTC 7 cut(s) 122, 683, 911, 1708, 1984, 2113, 2249
MmeI TCCRAC 6 cut(s) 43, 156, 1102, 1948, 2076, 2155
Mox20I TGGCCA 1 cut(s) 1493
Mph1103I ATGCAT 2 cut(s) 1288, 1504
MscI TGGCCA 1 cut(s) 1493
MseI TTAA 4 cut(s) 195, 962, 1197, 2296
MslI CAYNNNNRTG 2 cut(s) 369, 1778
Msp20I TGGCCA 1 cut(s) 1493
MspA1I CMGCKG 3 cut(s) 1210, 1643, 2156
MspI CCGG 5 cut(s) 119, 1321, 2058, 2097, 2199
MspR9I CCNGG 5 cut(s) 119, 1355, 1848, 1935, 2098
MunI CAATTG 2 cut(s) 1131, 1632
Mva1269I GAATGC 1 cut(s) 1470
MvaI CCWGG 3 cut(s) 1355, 1848, 1935
MvnI CGCG 2 cut(s) 56, 105
MwoI GCNNNNNNNGC 9 cut(s) 360, 497, 830, 1454, 1499, 1544, 1589, 1694, 2045
NciI CCSGG 2 cut(s) 119, 2098
NcoI CCATGG 1 cut(s) 708
NdeI CATATG 1 cut(s) 1504
NlaIV GGNNCC 8 cut(s) 337, 818, 892, 1639, 1672, 1939, 2014, 2128
NmuCI GTSAC 5 cut(s) 99, 845, 1261, 1517, 2165
NsiI ATGCAT 2 cut(s) 1288, 1504
NspI RCATGY 2 cut(s) 372, 2114
PagI TCATGA 1 cut(s) 955
PciI ACATGT 1 cut(s) 368
PcsI WCGNNNNNNNCGW 2 cut(s) 102, 204
PctI GAATGC 1 cut(s) 1470
PfeI GAWTC 7 cut(s) 37, 43, 66, 471, 1313, 1563, 1971
PflFI GACNNNGTC 1 cut(s) 2171
PinAI ACCGGT 1 cut(s) 2057
PleI GAGTC 7 cut(s) 122, 682, 910, 1708, 1984, 2113, 2248
PpsI GAGTC 7 cut(s) 122, 682, 910, 1708, 1984, 2113, 2248
Ppu21I YACGTR 2 cut(s) 97, 495
PscI ACATGT 1 cut(s) 368
Psp1406I AACGTT 1 cut(s) 207
Psp6I CCWGG 3 cut(s) 1353, 1846, 1933
PspFI CCCAGC 1 cut(s) 1241
PspGI CCWGG 3 cut(s) 1353, 1846, 1933
PspN4I GGNNCC 8 cut(s) 337, 818, 892, 1639, 1672, 1939, 2014, 2128
PspPI GGNCC 3 cut(s) 1508, 2126, 2201
PstI CTGCAG 1 cut(s) 1597
PstNI CAGNNNCTG 2 cut(s) 572, 1331
PsuI RGATCY 7 cut(s) 22, 701, 875, 890, 1896, 2012, 2140
PsyI GACNNNGTC 1 cut(s) 2171
PvuII CAGCTG 2 cut(s) 1210, 2156
RsaI GTAC 6 cut(s) 95, 427, 1110, 1824, 1864, 2031
RsaNI GTAC 6 cut(s) 94, 426, 1109, 1823, 1863, 2030
RseI CAYNNNNRTG 2 cut(s) 369, 1778
SaqAI TTAA 4 cut(s) 195, 962, 1197, 2296
Sau96I GGNCC 3 cut(s) 1508, 2126, 2201
ScaI AGTACT 1 cut(s) 1110
SchI GAGTC 7 cut(s) 122, 683, 911, 1708, 1984, 2113, 2249
ScrFI CCNGG 5 cut(s) 119, 1355, 1848, 1935, 2098
SduI GDGCHC 2 cut(s) 1475, 1942
SfaNI GCATC 6 cut(s) 747, 1489, 1570, 1579, 1897, 2057
SfcI CTRYAG 2 cut(s) 1449, 1593
SgrAI CRCCGGYG 1 cut(s) 2057
SinI GGWCC 2 cut(s) 1508, 2126
SmiMI CAYNNNNRTG 2 cut(s) 369, 1778
SmlI CTYRAG 1 cut(s) 2186
SmoI CTYRAG 1 cut(s) 2186
SpeI ACTAGT 1 cut(s) 356
Sse9I AATT 7 cut(s) 933, 1068, 1131, 1174, 1632, 1692, 1832
SsiI CCGC 8 cut(s) 32, 56, 144, 147, 1431, 1455, 1641, 2277
SspI AATATT 1 cut(s) 796
SspMI CTAG 2 cut(s) 357, 2246
StyD4I CCNGG 5 cut(s) 117, 1353, 1846, 1933, 2096
StyI CCWWGG 6 cut(s) 331, 475, 708, 812, 1459, 2130
TaaI ACNGT 5 cut(s) 1060, 1108, 1120, 1702, 2173
TaiI ACGT 3 cut(s) 99, 210, 497
TaqI TCGA 2 cut(s) 1901, 2238
TasI AATT 7 cut(s) 933, 1068, 1131, 1174, 1632, 1692, 1832
TatI WGTACW 2 cut(s) 1108, 2029
TauI GCSGC 3 cut(s) 149, 1433, 1457
TfiI GAWTC 7 cut(s) 37, 43, 66, 471, 1313, 1563, 1971
Tru1I TTAA 4 cut(s) 195, 962, 1197, 2296
Tru9I TTAA 4 cut(s) 195, 962, 1197, 2296
TscAI CASTG 3 cut(s) 92, 465, 1522
TseFI GTSAC 5 cut(s) 99, 845, 1261, 1517, 2165
Tsp45I GTSAC 5 cut(s) 99, 845, 1261, 1517, 2165
TspDTI ATGAA 5 cut(s) 396, 497, 930, 1717, 1796
TspGWI ACGGA 3 cut(s) 36, 287, 328
TspRI CASTG 3 cut(s) 92, 465, 1522
Tth111I GACNNNGTC 1 cut(s) 2171
VpaK11BI GGWCC 2 cut(s) 1508, 2126
XagI CCTNNNNNAGG 2 cut(s) 176, 1856
XceI RCATGY 2 cut(s) 372, 2114
XcmI CCANNNNNNNNNTGG 1 cut(s) 514
XspI CTAG 2 cut(s) 357, 2246
ZrmI AGTACT 1 cut(s) 1110
Zsp2I ATGCAT 2 cut(s) 1288, 1504
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.