pycom02g01810

Pyrophosphate-energized vacuolar membrane proton

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0000897
Physical Location & Seq
Forward (+)
1087086 .. 1091576
4491 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g01810.2

Sequence Viewer

Length: 2307 bp
ATGGGGGCGACGATACTCCCAGATCTCGGGGCCGAGATTTTGATCCCGCTGTGCGCGGTGGTCGGCATCGCCTTCTCCTTGGTTCAGTGGATGTACGTTTCGCAGGTCAAGCTCTCCCCCGGCGGCGGCCGGGACTCCAACTCCGGGAAAAATGGGCACAACGAGTACCTCATCGAGGAAGAAGAAGGCGGTAACGACCACAACGTCGTCCTCAAATGCGCCGAAATCCAAAACGCCATTTCAGAAGGAGCAACCTCGTTCCTCTTCACGGAATACAGATATGTAGGTGCTTTCATGGTTGCTTTTGCAATCTTGATCTTCCTTTTCCTTGGATCCGTCGAGGGATTTAGTACAAGTAGCCGTGCATGTACATATGACCAAACCAAGATGTGCAAACCGGCTCTCGCCACTGCCATCTTCAGCACCATTTCCTTTTTGCTTGGTGCTGTAACTTCAGTGGTTTCTGGATTCCTTGGGATGAAGATTGCTACTTACGCAAATGCCAGAACAACATTGGAAGCAAGAAAAGGTGTTGGGAAGGCTTTTATTACTGCATTTAGATCAGGAGCTGTGATGGGCTTTCTCCTCGCTTCAAGTGGTCTGTTGGTTCTTTACATTGCTATCAATGTCTTCAAGTTGTACTATGGTGATGACTGGGGTGGTCTTTTCGAGGCTATAACTGGCTATGGACTTGGTGGATCGATCACAGGAGCTTCCATGGCTCTCTTTGGCAGGGTTGGTGGAGGCATCTATACCAAAGCTGCTGATGTTGGTGCGGATCTTGTCGGCAAGGTTGAAAGAAATATTCCCGAGGATGACCCAAGGAACCCAGCCGTGATAGCTGATAATGTTGGCGACAATGTTGGAGATATAGCTGGCATGGGATCTGATCTTTTTGGATCCTATGCTGAGTCATCGTGTGCTGCCCTTGTGGTTGCTTCCATTTCCTCATTTGGGATCAATCACGAGCTAACTGCAATGCTATATCCTCTCATTGTCAGTTCCATCGGCATTCTTGTTTGTTTGCTCACTACCCTATTTGCAACCGATTTCTTTGAGATCAAGGCTGTAAAGGAAATTGAGCCAACATTGAAGAGGCAGCTCATAATCTCCACTGTACTCATGACCATTGGGGTTGCGATTGCTACATGGATTTCTGTTCCATCTTCCTTCACAATCTTCAATTTCGGAACACAGAAAGTTGTGAAGAACTGGCAGCTATTCTTATGTGTCTGTGTTGGTTTGTGGGCTGGCCTTATCATTGGGTTTGTAACAGAGTATTATACAAGCAACGCGTACAGCCCTGTGCAAGATGTTGCTGATTCTTGCAGGACTGGAGCTGCGACCAATGTCATTTTTGGCCTTGCATTGGGATACAAATCCGTCATTATTCCTATTTTTGCCATCGCAGTCAGCATTTTTGTTAGTTTTAGCTTTGCAGCTATGTACGGGATTGCTGTAGCTGCCCTTGGAATGCTGAGCACCATTGCAACTGGATTAGCGATTGATGCATATGGTCCCATCAGTGACAATGCTGGAGGCATTGCTGAGATGGCAGGAATGAGCCACAGAATCAGAGAGAGAACTGATGCTCTTGATGCTGCAGGAAACACCACTGCTGCTATTGGAAAGGGATTTGCAATTGGCTCTGCTGCTCTTGTGTCCCTTGCTCTATTTGGTGCCTTTGTCAGCCGTGCTGGTATCAAAACAGTTGATGTGTTGACCCCGAAAGTGATTATTGGTTTGTTAGTGGGCGCAATGCTTCCGTATTGGTTCTCTGCCATGACAATGAAGAGTGTGGGAAGTGCAGCTCTAAAGATGGTTGAGGAAGTGCGCCGGCAATTCAATACCATTCCAGGTCTCATGGAGGGCACTGCCAAGCCGGACTACGCTACATGTGTTAAGATTTCGACTGATGCTTCCATCAAAGAAATGATCCCACCTGGTGCCCTTGTGATGCTTACACCTCTCATTGTTGGGATCTTTTTTGGTGTGGAAACTCTCGCTGGCGTGCTTGCTGGATCCCTTGTCTCCGGTGTACAGATTGCCATTTCTGCGTCCAACACGGGCGGTGCCTGGGATAATGCCAAGAAATACATTGAGGCTGGTGCGTCGGAGCATGCAAGGACCCTTGGTCCAAAGGGATCAGAATGCCACAAAGCTGCTGTCATCGGTGACACCATTGGAGACCCTCTCAAGGTTACGTCTGGACCATCGCTCAACATTCTCATAAAGCTCATGGCCGTTGAGTCTCTTGTGTTTGCTCCGTTCTTTGCCACACACGGTGGCCTGCTTTTCAAGATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 map00190
Pfam Domains
Protein Families
H_PPase

Protein Analysis

769

Amino Acids

80.26

Weight (kDa)

5.57

Isoelectric Point (pI)

25.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 203
Acc36I ACCTGC 1 cut(s) 94
AccB1I GGYRCC 3 cut(s) 1679, 1946, 2072
AccII CGCG 2 cut(s) 56, 1295
AciI CCGC 7 cut(s) 47, 56, 123, 126, 189, 776, 2070
AcoI YGGCCR 2 cut(s) 127, 2241
AcuI CTGAAG 2 cut(s) 403, 438
AdeI CACNNNGTG 2 cut(s) 1946, 2285
AfaI GTAC 9 cut(s) 95, 167, 352, 370, 641, 1119, 1298, 1448, 2040
AfiI CCNNNNNNNGG 8 cut(s) 26, 125, 144, 175, 268, 954, 1369, 2197
AflIII ACRYGT 2 cut(s) 1293, 1895
AgsI TTSAA 7 cut(s) 594, 634, 797, 1093, 1183, 1846, 2299
AhdI GACNNNNNGTC 1 cut(s) 2133
AjnI CCWGG 3 cut(s) 1855, 1942, 2075
Alw21I GWGCWC 1 cut(s) 1484
Alw26I GTCTC 4 cut(s) 1865, 2035, 2181, 2256
AlwNI CAGNNNCTG 1 cut(s) 569
Ama87I CYCGRG 2 cut(s) 26, 809
AoxI GGCC 6 cut(s) 30, 127, 1252, 1360, 2241, 2287
ArsI GACNNNNNNTTYG 2 cut(s) 2151, 2183
AspLEI GCGC 4 cut(s) 56, 221, 1757, 1836
AspS9I GGNCC 5 cut(s) 30, 1517, 2127, 2135, 2210
AsuC2I CCSGG 3 cut(s) 120, 131, 145
AsuHPI GGTGA 2 cut(s) 659, 2186
AvaI CYCGRG 2 cut(s) 26, 809
AvaII GGWCC 4 cut(s) 1517, 2127, 2135, 2210
BaeGI GKGCMC 3 cut(s) 159, 1874, 1951
BamHI GGATCC 3 cut(s) 332, 899, 2021
BanI GGYRCC 3 cut(s) 1679, 1946, 2072
BauI CACGAG 1 cut(s) 965
BbsI GAAGAC 1 cut(s) 622
Bbv12I GWGCWC 1 cut(s) 1484
BceAI ACGGC 4 cut(s) 345, 818, 1677, 2228
BcgI CGANNNNNNTGC 2 cut(s) 956, 990
BciT130I CCWGG 3 cut(s) 1857, 1944, 2077
BciVI GTATCC 1 cut(s) 1367
BcnI CCSGG 3 cut(s) 120, 131, 145
BcoDI GTCTC 4 cut(s) 1865, 2035, 2181, 2256
BfmI CTRYAG 2 cut(s) 1458, 1602
BfuAI ACCTGC 1 cut(s) 94
BfuI GTATCC 1 cut(s) 1367
BglII AGATCT 1 cut(s) 22
BlpI GCTNAGC 1 cut(s) 1478
Bme1390I CCNGG 6 cut(s) 120, 131, 145, 1857, 1944, 2077
Bme18I GGWCC 4 cut(s) 1517, 2127, 2135, 2210
BmeRI GACNNNNNGTC 1 cut(s) 2133
BmeT110I CYCGRG 2 cut(s) 26, 809
BmgT120I GGNCC 5 cut(s) 30, 1517, 2127, 2135, 2210
BmrFI CCNGG 6 cut(s) 120, 131, 145, 1857, 1944, 2077
BmrI ACTGGG 1 cut(s) 664
BmsI GCATC 7 cut(s) 75, 756, 1498, 1579, 1588, 1906, 1947
BmuI ACTGGG 1 cut(s) 664
BoxI GACNNNNGTC 1 cut(s) 1349
BpiI GAAGAC 1 cut(s) 622
BplI GAGNNNNNCTC 2 cut(s) 2178, 2210
BpmI CTGGAG 2 cut(s) 1356, 1557
Bpu1102I GCTNAGC 1 cut(s) 1478
BpuEI CTTGAG 1 cut(s) 2180
BpuMI CCSGG 3 cut(s) 120, 131, 145
Bsa29I ATCGAT 1 cut(s) 701
BsaBI GATNNNNATC 2 cut(s) 41, 1378
BsaI GGTCTC 2 cut(s) 1865, 2181
BsaWI WCCGGW 1 cut(s) 2033
BsaXI ACNNNNNCTCC 2 cut(s) 125, 155
Bsc4I CCNNNNNNNGG 8 cut(s) 26, 125, 144, 175, 268, 954, 1369, 2197
Bse118I RCCGGY 2 cut(s) 397, 1836
Bse1I ACTGG 5 cut(s) 659, 685, 1217, 1339, 1498
Bse3DI GCAATG 5 cut(s) 615, 984, 1485, 1542, 1764
Bse8I GATNNNNATC 2 cut(s) 41, 1378
BseBI CCWGG 3 cut(s) 1857, 1944, 2077
BseCI ATCGAT 1 cut(s) 701
BseGI GGATG 3 cut(s) 96, 483, 820
BseJI GATNNNNATC 2 cut(s) 41, 1378
BseLI CCNNNNNNNGG 8 cut(s) 26, 125, 144, 175, 268, 954, 1369, 2197
BseMI GCAATG 5 cut(s) 615, 984, 1485, 1542, 1764
BseMII CTCAG 3 cut(s) 900, 1469, 1539
BseNI ACTGG 5 cut(s) 659, 685, 1217, 1339, 1498
BseRI GAGGAG 1 cut(s) 575
BseSI GKGCMC 3 cut(s) 159, 1874, 1951
BseX3I CGGCCG 1 cut(s) 127
BseYI CCCAGC 1 cut(s) 829
BsgI GTGCAG 1 cut(s) 1827
Bsh1236I CGCG 2 cut(s) 56, 1295
Bsh1285I CGRYCG 1 cut(s) 130
BshFI GGCC 6 cut(s) 32, 129, 1254, 1362, 2243, 2289
BshNI GGYRCC 3 cut(s) 1679, 1946, 2072
BshVI ATCGAT 1 cut(s) 701
BsiEI CGRYCG 1 cut(s) 130
BsiHKAI GWGCWC 1 cut(s) 1484
BsiHKCI CYCGRG 2 cut(s) 26, 809
BsiSI CCGG 7 cut(s) 120, 130, 144, 398, 1837, 1883, 2034
BslFI GGGAC 3 cut(s) 146, 1503, 1648
BslI CCNNNNNNNGG 8 cut(s) 26, 125, 144, 175, 268, 954, 1369, 2197
BsmAI GTCTC 4 cut(s) 1865, 2035, 2181, 2256
BsmFI GGGAC 3 cut(s) 146, 1503, 1648
BsmI GAATGC 3 cut(s) 1011, 1479, 2156
BsnI GGCC 6 cut(s) 32, 129, 1254, 1362, 2243, 2289
Bso31I GGTCTC 2 cut(s) 1865, 2181
BsoBI CYCGRG 2 cut(s) 26, 809
Bsp1286I GDGCHC 4 cut(s) 159, 1484, 1874, 1951
Bsp1407I TGTACA 2 cut(s) 368, 2038
Bsp1720I GCTNAGC 1 cut(s) 1478
Bsp19I CCATGG 1 cut(s) 717
BspACI CCGC 7 cut(s) 47, 56, 123, 126, 189, 776, 2070
BspANI GGCC 6 cut(s) 32, 129, 1254, 1362, 2243, 2289
BspCNI CTCAG 3 cut(s) 901, 1470, 1540
BspDI ATCGAT 1 cut(s) 701
BspFNI CGCG 2 cut(s) 56, 1295
BspHI TCATGA 1 cut(s) 1122
BspMAI CTGCAG 1 cut(s) 1606
BspMI ACCTGC 1 cut(s) 94
BspT107I GGYRCC 3 cut(s) 1679, 1946, 2072
BspTNI GGTCTC 2 cut(s) 1865, 2181
BsrDI GCAATG 5 cut(s) 615, 984, 1485, 1542, 1764
BsrFI RCCGGY 2 cut(s) 397, 1836
BsrGI TGTACA 2 cut(s) 368, 2038
BsrI ACTGG 5 cut(s) 659, 685, 1217, 1339, 1498
BssAI RCCGGY 2 cut(s) 397, 1836
BssSI CACGAG 1 cut(s) 965
BssT1I CCWWGG 7 cut(s) 78, 328, 472, 717, 821, 1468, 2131
Bst2BI CACGAG 1 cut(s) 965
Bst2UI CCWGG 3 cut(s) 1857, 1944, 2077
Bst4CI ACNGT 3 cut(s) 1117, 1711, 2285
Bst6I CTCTTC 3 cut(s) 269, 1088, 1787
BstAUI TGTACA 2 cut(s) 368, 2038
BstC8I GCNNGC 8 cut(s) 877, 1252, 1838, 2008, 2012, 2016, 2121, 2291
BstDEI CTNAG 3 cut(s) 909, 1478, 1548
BstDSI CCRYGG 1 cut(s) 717
BstENI CCTNNNNNAGG 1 cut(s) 173
BstF5I GGATG 3 cut(s) 96, 483, 820
BstFNI CGCG 2 cut(s) 56, 1295
BstHHI GCGC 4 cut(s) 56, 221, 1757, 1836
BstMAI GTCTC 4 cut(s) 1865, 2035, 2181, 2256
BstMCI CGRYCG 1 cut(s) 130
BstMWI GCNNNNNNNGC 9 cut(s) 109, 494, 719, 839, 1463, 1508, 1553, 1598, 2054
BstNI CCWGG 3 cut(s) 1857, 1944, 2077
BstNSI RCATGY 3 cut(s) 369, 1899, 2123
BstPAI GACNNNNGTC 1 cut(s) 1349
BstSCI CCNGG 6 cut(s) 118, 129, 143, 1855, 1942, 2075
BstSFI CTRYAG 2 cut(s) 1458, 1602
BstSLI GKGCMC 3 cut(s) 159, 1874, 1951
BstUI CGCG 2 cut(s) 56, 1295
BstV2I GAAGAC 1 cut(s) 622
BstX2I RGATCY 7 cut(s) 22, 332, 778, 884, 899, 1980, 2021
BstYI RGATCY 7 cut(s) 22, 332, 778, 884, 899, 1980, 2021
BstZI CGGCCG 1 cut(s) 127
Bsu15I ATCGAT 1 cut(s) 701
BsuI GTATCC 1 cut(s) 1367
BsuRI GGCC 6 cut(s) 32, 129, 1254, 1362, 2243, 2289
BsuTUI ATCGAT 1 cut(s) 701
BtgI CCRYGG 1 cut(s) 717
BtgZI GCGATG 3 cut(s) 52, 1390, 2199
BtsCI GGATG 3 cut(s) 96, 483, 820
BtsI GCAGTG 3 cut(s) 408, 1614, 1872
BtsIMutI CAGTG 7 cut(s) 92, 408, 462, 1113, 1531, 1614, 1872
BveI ACCTGC 1 cut(s) 94
Cac8I GCNNGC 8 cut(s) 877, 1252, 1838, 2008, 2012, 2016, 2121, 2291
CaiI CAGNNNCTG 1 cut(s) 569
CciI TCATGA 1 cut(s) 1122
CfoI GCGC 4 cut(s) 56, 221, 1757, 1836
Cfr10I RCCGGY 2 cut(s) 397, 1836
Cfr13I GGNCC 5 cut(s) 30, 1517, 2127, 2135, 2210
ClaI ATCGAT 1 cut(s) 701
CseI GACGC 2 cut(s) 2046, 2100
CsiI ACCWGGT 1 cut(s) 1942
Csp6I GTAC 9 cut(s) 94, 166, 351, 369, 640, 1118, 1297, 1447, 2039
CviQI GTAC 9 cut(s) 94, 166, 351, 369, 640, 1118, 1297, 1447, 2039
DdeI CTNAG 3 cut(s) 909, 1478, 1548
DraIII CACNNNGTG 2 cut(s) 1946, 2285
DrdI GACNNNNNNGTC 1 cut(s) 203
DriI GACNNNNNGTC 1 cut(s) 2133
DseDI GACNNNNNNGTC 1 cut(s) 203
EaeI YGGCCR 2 cut(s) 127, 2241
EagI CGGCCG 1 cut(s) 127
Eam1104I CTCTTC 3 cut(s) 269, 1088, 1787
Eam1105I GACNNNNNGTC 1 cut(s) 2133
EarI CTCTTC 3 cut(s) 269, 1088, 1787
EclXI CGGCCG 1 cut(s) 127
Eco130I CCWWGG 7 cut(s) 78, 328, 472, 717, 821, 1468, 2131
Eco31I GGTCTC 2 cut(s) 1865, 2181
Eco47I GGWCC 4 cut(s) 1517, 2127, 2135, 2210
Eco52I CGGCCG 1 cut(s) 127
Eco57I CTGAAG 2 cut(s) 403, 438
Eco88I CYCGRG 2 cut(s) 26, 809
EcoNI CCTNNNNNAGG 1 cut(s) 173
EcoO109I RGGNCCY 1 cut(s) 2127
EcoRII CCWGG 3 cut(s) 1855, 1942, 2075
EcoT14I CCWWGG 7 cut(s) 78, 328, 472, 717, 821, 1468, 2131
EcoT22I ATGCAT 1 cut(s) 1513
ErhI CCWWGG 7 cut(s) 78, 328, 472, 717, 821, 1468, 2131
FaqI GGGAC 3 cut(s) 146, 1503, 1648
FauI CCCGC 1 cut(s) 54
FauNDI CATATG 2 cut(s) 373, 1513
FokI GGATG 3 cut(s) 103, 490, 827
GlaI GCGC 4 cut(s) 55, 220, 1756, 1835
GsaI CCCAGC 1 cut(s) 833
GsuI CTGGAG 2 cut(s) 1356, 1557
HaeIII GGCC 6 cut(s) 32, 129, 1254, 1362, 2243, 2289
HapII CCGG 7 cut(s) 120, 130, 144, 398, 1837, 1883, 2034
HgaI GACGC 2 cut(s) 2046, 2100
HhaI GCGC 4 cut(s) 56, 221, 1757, 1836
Hin6I GCGC 4 cut(s) 54, 219, 1755, 1834
HinP1I GCGC 4 cut(s) 54, 219, 1755, 1834
HincII GTYRAC 1 cut(s) 1722
HindII GTYRAC 1 cut(s) 1722
HinfI GANTC 6 cut(s) 134, 468, 911, 1322, 1572, 2249
HpaII CCGG 7 cut(s) 120, 130, 144, 398, 1837, 1883, 2034
HphI GGTGA 2 cut(s) 659, 2186
Hpy166II GTNNAC 2 cut(s) 1722, 2039
Hpy188I TCNGA 6 cut(s) 244, 889, 1190, 1577, 2116, 2149
Hpy188III TCNNGA 9 cut(s) 313, 465, 564, 809, 965, 1123, 1595, 2208, 2299
Hpy8I GTNNAC 2 cut(s) 1722, 2039
Hpy99I CGWCG 4 cut(s) 13, 209, 341, 2116
HpyAV CCTTC 5 cut(s) 82, 179, 239, 532, 1180
HpyCH4III ACNGT 3 cut(s) 1117, 1711, 2285
HpyCH4IV ACGT 3 cut(s) 96, 204, 2204
HpyF10VI GCNNNNNNNGC 9 cut(s) 109, 494, 719, 839, 1463, 1508, 1553, 1598, 2054
HpyF3I CTNAG 3 cut(s) 909, 1478, 1548
HpySE526I ACGT 3 cut(s) 96, 204, 2204
HspAI GCGC 4 cut(s) 54, 219, 1755, 1834
KroI GCCGGC 1 cut(s) 1836
KroNI GCCGGC 1 cut(s) 1838
LmnI GCTCC 6 cut(s) 248, 566, 710, 1337, 2116, 2269
LweI GCATC 7 cut(s) 75, 756, 1498, 1579, 1588, 1906, 1947
MabI ACCWGGT 1 cut(s) 1942
MaeII ACGT 3 cut(s) 96, 204, 2204
MaeIII GTNAC 6 cut(s) 191, 448, 1270, 1526, 2174, 2200
MfeI CAATTG 1 cut(s) 1641
MflI RGATCY 7 cut(s) 22, 332, 778, 884, 899, 1980, 2021
MhlI GDGCHC 4 cut(s) 159, 1484, 1874, 1951
MluCI AATT 4 cut(s) 1077, 1183, 1641, 1841
MluI ACGCGT 1 cut(s) 1293
MlyI GAGTC 3 cut(s) 128, 920, 2258
MmeI TCCRAC 4 cut(s) 162, 844, 2085, 2094
Mph1103I ATGCAT 1 cut(s) 1513
MroNI GCCGGC 1 cut(s) 1836
MseI TTAA 2 cut(s) 1902, 2305
MslI CAYNNNNRTG 1 cut(s) 1787
MspA1I CMGCKG 1 cut(s) 49
MspI CCGG 7 cut(s) 120, 130, 144, 398, 1837, 1883, 2034
MspR9I CCNGG 6 cut(s) 120, 131, 145, 1857, 1944, 2077
MunI CAATTG 1 cut(s) 1641
Mva1269I GAATGC 3 cut(s) 1011, 1479, 2156
MvaI CCWGG 3 cut(s) 1857, 1944, 2077
MvnI CGCG 2 cut(s) 56, 1295
MwoI GCNNNNNNNGC 9 cut(s) 109, 494, 719, 839, 1463, 1508, 1553, 1598, 2054
NaeI GCCGGC 1 cut(s) 1838
NciI CCSGG 3 cut(s) 120, 131, 145
NcoI CCATGG 1 cut(s) 717
NdeI CATATG 2 cut(s) 373, 1513
NgoMIV GCCGGC 1 cut(s) 1836
NmeAIII GCCGAG 1 cut(s) 58
NmuCI GTSAC 2 cut(s) 1526, 2174
NsiI ATGCAT 1 cut(s) 1513
NspI RCATGY 3 cut(s) 369, 1899, 2123
PaeI GCATGC 1 cut(s) 2123
PagI TCATGA 1 cut(s) 1122
PciI ACATGT 1 cut(s) 1895
PcsI WCGNNNNNNNCGW 1 cut(s) 201
PctI GAATGC 3 cut(s) 1011, 1479, 2156
PdiI GCCGGC 1 cut(s) 1838
PfeI GAWTC 3 cut(s) 468, 1322, 1572
PfoI TCCNGGA 1 cut(s) 143
PleI GAGTC 3 cut(s) 128, 919, 2257
PpsI GAGTC 3 cut(s) 128, 919, 2257
PpuMI RGGWCCY 1 cut(s) 2127
PscI ACATGT 1 cut(s) 1895
PshAI GACNNNNGTC 1 cut(s) 1349
Psp5II RGGWCCY 1 cut(s) 2127
Psp6I CCWGG 3 cut(s) 1855, 1942, 2075
PspFI CCCAGC 1 cut(s) 829
PspGI CCWGG 3 cut(s) 1855, 1942, 2075
PspPI GGNCC 5 cut(s) 30, 1517, 2127, 2135, 2210
PspPPI RGGWCCY 1 cut(s) 2127
PstI CTGCAG 1 cut(s) 1606
PstNI CAGNNNCTG 1 cut(s) 569
PsuI RGATCY 7 cut(s) 22, 332, 778, 884, 899, 1980, 2021
RsaI GTAC 9 cut(s) 95, 167, 352, 370, 641, 1119, 1298, 1448, 2040
RsaNI GTAC 9 cut(s) 94, 166, 351, 369, 640, 1118, 1297, 1447, 2039
RseI CAYNNNNRTG 1 cut(s) 1787
SaqAI TTAA 2 cut(s) 1902, 2305
Sau96I GGNCC 5 cut(s) 30, 1517, 2127, 2135, 2210
SchI GAGTC 3 cut(s) 128, 920, 2258
ScrFI CCNGG 6 cut(s) 120, 131, 145, 1857, 1944, 2077
SduI GDGCHC 4 cut(s) 159, 1484, 1874, 1951
SexAI ACCWGGT 1 cut(s) 1942
SfaNI GCATC 7 cut(s) 75, 756, 1498, 1579, 1588, 1906, 1947
SfcI CTRYAG 2 cut(s) 1458, 1602
SinI GGWCC 4 cut(s) 1517, 2127, 2135, 2210
SmiMI CAYNNNNRTG 1 cut(s) 1787
SmlI CTYRAG 1 cut(s) 2195
SmoI CTYRAG 1 cut(s) 2195
SphI GCATGC 1 cut(s) 2123
Sse9I AATT 4 cut(s) 1077, 1183, 1641, 1841
SsiI CCGC 7 cut(s) 47, 56, 123, 126, 189, 776, 2070
SspI AATATT 1 cut(s) 805
StyD4I CCNGG 6 cut(s) 118, 129, 143, 1855, 1942, 2075
StyI CCWWGG 7 cut(s) 78, 328, 472, 717, 821, 1468, 2131
TaaI ACNGT 3 cut(s) 1117, 1711, 2285
TaiI ACGT 3 cut(s) 99, 207, 2207
TaqI TCGA 5 cut(s) 174, 339, 669, 701, 1910
TasI AATT 4 cut(s) 1077, 1183, 1641, 1841
TatI WGTACW 5 cut(s) 350, 368, 639, 1117, 2038
TauI GCSGC 2 cut(s) 126, 129
TfiI GAWTC 3 cut(s) 468, 1322, 1572
Tru1I TTAA 2 cut(s) 1902, 2305
Tru9I TTAA 2 cut(s) 1902, 2305
TscAI CASTG 7 cut(s) 92, 415, 462, 1120, 1531, 1621, 1879
TseFI GTSAC 2 cut(s) 1526, 2174
Tsp45I GTSAC 2 cut(s) 1526, 2174
TspDTI ATGAA 3 cut(s) 283, 494, 1805
TspGWI ACGGA 5 cut(s) 284, 325, 1372, 1755, 2256
TspRI CASTG 7 cut(s) 92, 415, 462, 1120, 1531, 1621, 1879
VpaK11BI GGWCC 4 cut(s) 1517, 2127, 2135, 2210
XagI CCTNNNNNAGG 1 cut(s) 173
XceI RCATGY 3 cut(s) 369, 1899, 2123
XcmI CCANNNNNNNNNTGG 1 cut(s) 511
Zsp2I ATGCAT 1 cut(s) 1513
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.