Rmu_sc0017478.1_g000001

Pyrophosphate-energized vacuolar membrane proton

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0017478.1
OG0000897
Physical Location & Seq
Forward (+)
1 .. 2601
2601 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0017478.1_g000001.1.cds

Sequence Viewer

Length: 1832 bp
gagcaacatcattccttttcacggaatataagtatgttggtgcttttatggtggcttttgcagtcttgatatttgttttccttggctccgtggagggatttagcactagtagccatacatgtgcttatgatgaaacaaagatatgcaaacctgctcttgcaaccgccatattcagtaccatttcctttttgcttggtgctataacttccgtggtttctggattccttggtatgaagattgccacgtatgcaaatgccagaactactttggaggcaagaaaaggtgttgggaaggcttttatagtagcatttagatcaggtgctgtcatgggatttctccttgctgcaaatggtttattagttttatacattgtgatccttgtcttcaagatgttctatggtgaggattggggtggtctttttgagtccataactgggtatggtcttggtggatcttccatggctctttttggcagagttggtggaggcatctataccaaagctgctgatgtcggtgctgatcttgttggcaaagttgaaaggaatattcctgaggatgacccaaggaaccctgctgtgattgctgataatgttggtgacaatgttggggatattgctggaatgggatctgatctctttggatcctatgctgagtcatcatgtgctgctcttgttgttgcttcaatttcttcatttgggatcaatcatgagttaactgcaatgttatatcctctcattgtcagttccattggtatcattgtttgtctgatcaccacccttttcgcaactgatttctttgagatcaagactgtaaaggaaattgagccagcattgaagaagcaactcattatttccacagtactgatgactgttggagttgcaattgttacttggatttctgttccttcttccttcaccatcttcaattttggaacacagaaggttgttaagaactggcagctgttcttgtgcgtgtgtgttgggttgtgggctgggcttatcatcggatttgtgacagagtattataccagtaatgcatacagccctgtgcaagatgttgctgattcgtgccggacaggagctgctactaatatcatatttggcctggcattgggatacaaatctgtcattattcctatttttgccattgctgttagtatttttgttagttttagctttgccgctatgtatggcattgctgtagccgcccttggaatgctgagcaccatagcaactggattggccattgatgcatatggtccgatcagtgacaatgctggtggcattgctgagatggcaggcatgagccacagaatcagagagagaactgatgctcttgatgctgcaggaaacaccacggctgctattggaaagggttttgcaattggctccgctgctcttgtgtcccttgctctctttggtgcctttgtgagccgtgctggaattgcaacagttgatgtgttgactccaaaagtgttcattggtttgatagtgggtgcaatgcttccctattggttctctgccatgacaatgaagagtgtgggaagtgctgctctaaagatggttgaagaagtacgcaggcaattcaacaccatccctggccttatggagggtactgctaaacctgactatgctacttgcgtcaagatctcgactgatgcttccatcaaggaaatgatccctcctggtgcccttgtcatgctaacacccctcattgtcggaatcttttttggtgtggagactctctctggtgtccttgctggatccctcgtctctggtgtacaggtaaattcttcagcactggctggattaaaatcttctgtatgcatataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 map00190
Pfam Domains
Protein Families
H_PPase

Protein Analysis

609

Amino Acids

63.61

Weight (kDa)

5.61

Isoelectric Point (pI)

19.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 159
AccB1I GGYRCC 2 cut(s) 1420, 1687
AciI CCGC 4 cut(s) 164, 1181, 1205, 1391
AclWI GGATC 9 cut(s) 369, 459, 633, 635, 648, 706, 1671, 1757, 1770
AcoI YGGCCR 1 cut(s) 1241
AcsI RAATTY 1 cut(s) 1788
AcuI CTGAAG 1 cut(s) 1778
AfaI GTAC 5 cut(s) 177, 860, 1574, 1614, 1781
AfiI CCNNNNNNNGG 4 cut(s) 21, 434, 1110, 1608
AflIII ACRYGT 1 cut(s) 118
AgsI TTSAA 7 cut(s) 387, 538, 683, 834, 924, 1567, 1587
AhlI ACTAGT 1 cut(s) 106
AjnI CCWGG 3 cut(s) 1103, 1596, 1683
AluBI AGCT 4 cut(s) 502, 960, 1081, 1175
AluI AGCT 4 cut(s) 502, 960, 1081, 1175
Alw21I GWGCWC 1 cut(s) 1225
Alw26I GTCTC 2 cut(s) 1732, 1776
AlwI GGATC 9 cut(s) 369, 459, 633, 635, 648, 706, 1671, 1757, 1770
AlwNI CAGNNNCTG 2 cut(s) 322, 1081
AoxI GGCC 3 cut(s) 1101, 1241, 1599
ApeKI GCWGC 9 cut(s) 343, 502, 664, 957, 1081, 1342, 1360, 1393, 1549
ApoI RAATTY 1 cut(s) 1788
AspS9I GGNCC 1 cut(s) 1258
AsuHPI GGTGA 4 cut(s) 412, 607, 762, 906
AvaII GGWCC 1 cut(s) 1258
AxyI CCTNAGG 1 cut(s) 551
BaeGI GKGCMC 1 cut(s) 1692
BalI TGGCCA 1 cut(s) 1243
BamHI GGATCC 2 cut(s) 640, 1762
BanI GGYRCC 2 cut(s) 1420, 1687
BbsI GAAGAC 1 cut(s) 375
Bbv12I GWGCWC 1 cut(s) 1225
BbvI GCAGC 9 cut(s) 330, 489, 651, 969, 1068, 1329, 1347, 1380, 1536
BccI CCATC 5 cut(s) 925, 1287, 1554, 1600, 1672
BceAI ACGGC 2 cut(s) 1373, 1418
BciT130I CCWGG 3 cut(s) 1105, 1598, 1685
BciVI GTATCC 1 cut(s) 1108
BclI TGATCA 1 cut(s) 767
BcoDI GTCTC 2 cut(s) 1732, 1776
BcuI ACTAGT 1 cut(s) 106
BfaI CTAG 1 cut(s) 107
BfmI CTRYAG 2 cut(s) 1199, 1343
BfuAI ACCTGC 1 cut(s) 159
BfuI GTATCC 1 cut(s) 1108
BglII AGATCT 1 cut(s) 1646
BlpI GCTNAGC 1 cut(s) 1219
BmcAI AGTACT 1 cut(s) 860
Bme1390I CCNGG 3 cut(s) 1105, 1598, 1685
Bme18I GGWCC 1 cut(s) 1258
BmgT120I GGNCC 1 cut(s) 1258
BmiI GGNNCC 7 cut(s) 87, 568, 642, 1389, 1422, 1689, 1764
BmrFI CCNGG 3 cut(s) 1105, 1598, 1685
BmrI ACTGGG 1 cut(s) 443
BmsI GCATC 5 cut(s) 497, 1239, 1320, 1329, 1647
BmuI ACTGGG 1 cut(s) 443
BpiI GAAGAC 1 cut(s) 375
BplI GAGNNNNNCTC 2 cut(s) 1729, 1761
Bpu1102I GCTNAGC 1 cut(s) 1219
BsaAI YACGTR 1 cut(s) 245
BsaBI GATNNNNATC 2 cut(s) 1119, 1812
BsaJI CCNNGG 9 cut(s) 81, 89, 209, 225, 458, 562, 1209, 1355, 1596
Bsc4I CCNNNNNNNGG 4 cut(s) 21, 434, 1110, 1608
Bse1I ACTGG 5 cut(s) 438, 958, 1028, 1239, 1805
Bse21I CCTNAGG 1 cut(s) 551
Bse3DI GCAATG 5 cut(s) 725, 1145, 1193, 1283, 1505
Bse8I GATNNNNATC 2 cut(s) 1119, 1812
BseBI CCWGG 3 cut(s) 1105, 1598, 1685
BseDI CCNNGG 9 cut(s) 81, 89, 209, 225, 458, 562, 1209, 1355, 1596
BseGI GGATG 2 cut(s) 561, 1592
BseJI GATNNNNATC 2 cut(s) 1119, 1812
BseLI CCNNNNNNNGG 4 cut(s) 21, 434, 1110, 1608
BseMI GCAATG 5 cut(s) 725, 1145, 1193, 1283, 1505
BseMII CTCAG 4 cut(s) 542, 641, 1210, 1280
BseNI ACTGG 5 cut(s) 438, 958, 1028, 1239, 1805
BseSI GKGCMC 1 cut(s) 1692
BseXI GCAGC 9 cut(s) 330, 489, 651, 969, 1068, 1329, 1347, 1380, 1536
BseYI CCCAGC 1 cut(s) 991
BshFI GGCC 3 cut(s) 1103, 1243, 1601
BshNI GGYRCC 2 cut(s) 1420, 1687
BsiHKAI GWGCWC 1 cut(s) 1225
BsiSI CCGG 1 cut(s) 1071
BslFI GGGAC 1 cut(s) 1389
BslI CCNNNNNNNGG 4 cut(s) 21, 434, 1110, 1608
BsmAI GTCTC 2 cut(s) 1732, 1776
BsmBI CGTCTC 1 cut(s) 1776
BsmFI GGGAC 1 cut(s) 1389
BsmI GAATGC 1 cut(s) 1220
BsnI GGCC 3 cut(s) 1103, 1243, 1601
Bsp1286I GDGCHC 2 cut(s) 1225, 1692
Bsp1407I TGTACA 1 cut(s) 1779
Bsp1720I GCTNAGC 1 cut(s) 1219
Bsp19I CCATGG 1 cut(s) 458
BspACI CCGC 4 cut(s) 164, 1181, 1205, 1391
BspANI GGCC 3 cut(s) 1103, 1243, 1601
BspCNI CTCAG 4 cut(s) 543, 642, 1211, 1281
BspHI TCATGA 1 cut(s) 705
BspLI GGNNCC 7 cut(s) 87, 568, 642, 1389, 1422, 1689, 1764
BspMAI CTGCAG 1 cut(s) 1347
BspMI ACCTGC 1 cut(s) 159
BspPI GGATC 9 cut(s) 369, 459, 633, 635, 648, 706, 1671, 1757, 1770
BspT107I GGYRCC 2 cut(s) 1420, 1687
BsrDI GCAATG 5 cut(s) 725, 1145, 1193, 1283, 1505
BsrGI TGTACA 1 cut(s) 1779
BsrI ACTGG 5 cut(s) 438, 958, 1028, 1239, 1805
BssECI CCNNGG 9 cut(s) 81, 89, 209, 225, 458, 562, 1209, 1355, 1596
BssT1I CCWWGG 5 cut(s) 81, 225, 458, 562, 1209
Bst2UI CCWGG 3 cut(s) 1105, 1598, 1685
Bst4CI ACNGT 4 cut(s) 810, 858, 870, 1452
Bst6I CTCTTC 1 cut(s) 1528
BstAUI TGTACA 1 cut(s) 1779
BstBAI YACGTR 1 cut(s) 245
BstC8I GCNNGC 3 cut(s) 827, 1299, 1579
BstDEI CTNAG 4 cut(s) 551, 650, 1219, 1289
BstDSI CCRYGG 4 cut(s) 89, 209, 458, 1355
BstENI CCTNNNNNAGG 1 cut(s) 1606
BstF5I GGATG 2 cut(s) 561, 1592
BstMAI GTCTC 2 cut(s) 1732, 1776
BstMWI GCNNNNNNNGC 8 cut(s) 110, 247, 580, 1204, 1249, 1294, 1339, 1444
BstNI CCWGG 3 cut(s) 1105, 1598, 1685
BstNSI RCATGY 1 cut(s) 122
BstSCI CCNGG 3 cut(s) 1103, 1596, 1683
BstSFI CTRYAG 2 cut(s) 1199, 1343
BstSLI GKGCMC 1 cut(s) 1692
BstV1I GCAGC 9 cut(s) 330, 489, 651, 969, 1068, 1329, 1347, 1380, 1536
BstV2I GAAGAC 1 cut(s) 375
BstX2I RGATCY 5 cut(s) 451, 625, 640, 1646, 1762
BstYI RGATCY 5 cut(s) 451, 625, 640, 1646, 1762
Bsu36I CCTNAGG 1 cut(s) 551
BsuI GTATCC 1 cut(s) 1108
BsuRI GGCC 3 cut(s) 1103, 1243, 1601
BtgI CCRYGG 4 cut(s) 89, 209, 458, 1355
BtsCI GGATG 2 cut(s) 561, 1592
BtsIMutI CAGTG 2 cut(s) 1272, 1798
BveI ACCTGC 1 cut(s) 159
Cac8I GCNNGC 3 cut(s) 827, 1299, 1579
CaiI CAGNNNCTG 2 cut(s) 322, 1081
CciI TCATGA 1 cut(s) 705
Cfr13I GGNCC 1 cut(s) 1258
CseI GACGC 1 cut(s) 1629
Csp6I GTAC 5 cut(s) 176, 859, 1573, 1613, 1780
CspCI CAANNNNNGTGG 2 cut(s) 1260, 1295
CviAII CATG 8 cut(s) 119, 327, 459, 659, 706, 1302, 1524, 1698
CviQI GTAC 5 cut(s) 176, 859, 1573, 1613, 1780
DdeI CTNAG 4 cut(s) 551, 650, 1219, 1289
EaeI YGGCCR 1 cut(s) 1241
Eam1104I CTCTTC 1 cut(s) 1528
EarI CTCTTC 1 cut(s) 1528
Eco130I CCWWGG 5 cut(s) 81, 225, 458, 562, 1209
Eco47I GGWCC 1 cut(s) 1258
Eco57I CTGAAG 1 cut(s) 1778
Eco81I CCTNAGG 1 cut(s) 551
EcoNI CCTNNNNNAGG 1 cut(s) 1606
EcoRII CCWGG 3 cut(s) 1103, 1596, 1683
EcoT14I CCWWGG 5 cut(s) 81, 225, 458, 562, 1209
EcoT22I ATGCAT 3 cut(s) 1038, 1254, 1828
ErhI CCWWGG 5 cut(s) 81, 225, 458, 562, 1209
Esp3I CGTCTC 1 cut(s) 1776
FaeI CATG 8 cut(s) 122, 330, 462, 662, 709, 1305, 1527, 1701
FaqI GGGAC 1 cut(s) 1389
FatI CATG 8 cut(s) 118, 326, 458, 658, 705, 1301, 1523, 1697
FauNDI CATATG 1 cut(s) 1254
FbaI TGATCA 1 cut(s) 767
FokI GGATG 2 cut(s) 568, 1579
FspBI CTAG 1 cut(s) 107
GsaI CCCAGC 1 cut(s) 995
HaeIII GGCC 3 cut(s) 1103, 1243, 1601
HapII CCGG 1 cut(s) 1071
HgaI GACGC 1 cut(s) 1629
Hin1II CATG 8 cut(s) 122, 330, 462, 662, 709, 1305, 1527, 1701
HincII GTYRAC 2 cut(s) 713, 1463
HindII GTYRAC 2 cut(s) 713, 1463
HinfI GANTC 8 cut(s) 221, 424, 652, 1063, 1313, 1464, 1721, 1740
HpaI GTTAAC 1 cut(s) 713
HpaII CCGG 1 cut(s) 1071
HphI GGTGA 4 cut(s) 412, 607, 762, 906
Hpy166II GTNNAC 3 cut(s) 713, 1463, 1780
Hpy188I TCNGA 6 cut(s) 630, 767, 1006, 1262, 1318, 1720
Hpy188III TCNNGA 9 cut(s) 66, 218, 387, 550, 706, 804, 1336, 1644, 1650
Hpy8I GTNNAC 3 cut(s) 713, 1463, 1780
HpyAV CCTTC 4 cut(s) 285, 914, 921, 933
HpyCH4III ACNGT 4 cut(s) 810, 858, 870, 1452
HpyCH4IV ACGT 1 cut(s) 244
HpyF10VI GCNNNNNNNGC 8 cut(s) 110, 247, 580, 1204, 1249, 1294, 1339, 1444
HpyF3I CTNAG 4 cut(s) 551, 650, 1219, 1289
HpySE526I ACGT 1 cut(s) 244
Hsp92II CATG 8 cut(s) 122, 330, 462, 662, 709, 1305, 1527, 1701
Ksp22I TGATCA 1 cut(s) 767
KspAI GTTAAC 1 cut(s) 713
LmnI GCTCC 3 cut(s) 91, 1078, 1393
Lsp1109I GCAGC 9 cut(s) 330, 489, 651, 969, 1068, 1329, 1347, 1380, 1536
LweI GCATC 5 cut(s) 497, 1239, 1320, 1329, 1647
MaeI CTAG 1 cut(s) 107
MaeII ACGT 1 cut(s) 244
MaeIII GTNAC 4 cut(s) 595, 885, 1011, 1267
MfeI CAATTG 2 cut(s) 881, 1382
MflI RGATCY 5 cut(s) 451, 625, 640, 1646, 1762
MhlI GDGCHC 2 cut(s) 1225, 1692
MlsI TGGCCA 1 cut(s) 1243
MluCI AATT 8 cut(s) 683, 818, 881, 924, 1382, 1442, 1582, 1788
MluNI TGGCCA 1 cut(s) 1243
MlyI GAGTC 4 cut(s) 433, 661, 1458, 1734
MmeI TCCRAC 2 cut(s) 852, 1698
Mox20I TGGCCA 1 cut(s) 1243
Mph1103I ATGCAT 3 cut(s) 1038, 1254, 1828
MscI TGGCCA 1 cut(s) 1243
MseI TTAA 3 cut(s) 712, 947, 1810
MslI CAYNNNNRTG 2 cut(s) 119, 1528
Msp20I TGGCCA 1 cut(s) 1243
MspA1I CMGCKG 2 cut(s) 960, 1393
MspI CCGG 1 cut(s) 1071
MspR9I CCNGG 3 cut(s) 1105, 1598, 1685
MunI CAATTG 2 cut(s) 881, 1382
Mva1269I GAATGC 1 cut(s) 1220
MvaI CCWGG 3 cut(s) 1105, 1598, 1685
MwoI GCNNNNNNNGC 8 cut(s) 110, 247, 580, 1204, 1249, 1294, 1339, 1444
NcoI CCATGG 1 cut(s) 458
NdeI CATATG 1 cut(s) 1254
NlaIII CATG 8 cut(s) 122, 330, 462, 662, 709, 1305, 1527, 1701
NlaIV GGNNCC 7 cut(s) 87, 568, 642, 1389, 1422, 1689, 1764
NmuCI GTSAC 3 cut(s) 595, 1011, 1267
NsiI ATGCAT 3 cut(s) 1038, 1254, 1828
NspI RCATGY 1 cut(s) 122
PagI TCATGA 1 cut(s) 705
PciI ACATGT 1 cut(s) 118
PctI GAATGC 1 cut(s) 1220
PfeI GAWTC 4 cut(s) 221, 1063, 1313, 1721
PleI GAGTC 4 cut(s) 432, 660, 1458, 1734
PpsI GAGTC 4 cut(s) 432, 660, 1458, 1734
Ppu21I YACGTR 1 cut(s) 245
PscI ACATGT 1 cut(s) 118
Psp6I CCWGG 3 cut(s) 1103, 1596, 1683
PspFI CCCAGC 1 cut(s) 991
PspGI CCWGG 3 cut(s) 1103, 1596, 1683
PspN4I GGNNCC 7 cut(s) 87, 568, 642, 1389, 1422, 1689, 1764
PspPI GGNCC 1 cut(s) 1258
PstI CTGCAG 1 cut(s) 1347
PstNI CAGNNNCTG 2 cut(s) 322, 1081
PsuI RGATCY 5 cut(s) 451, 625, 640, 1646, 1762
PvuII CAGCTG 1 cut(s) 960
RsaI GTAC 5 cut(s) 177, 860, 1574, 1614, 1781
RsaNI GTAC 5 cut(s) 176, 859, 1573, 1613, 1780
RseI CAYNNNNRTG 2 cut(s) 119, 1528
SaqAI TTAA 3 cut(s) 712, 947, 1810
Sau96I GGNCC 1 cut(s) 1258
ScaI AGTACT 1 cut(s) 860
SchI GAGTC 4 cut(s) 433, 661, 1458, 1734
ScrFI CCNGG 3 cut(s) 1105, 1598, 1685
SduI GDGCHC 2 cut(s) 1225, 1692
SfaNI GCATC 5 cut(s) 497, 1239, 1320, 1329, 1647
SfcI CTRYAG 2 cut(s) 1199, 1343
SinI GGWCC 1 cut(s) 1258
SmiMI CAYNNNNRTG 2 cut(s) 119, 1528
SpeI ACTAGT 1 cut(s) 106
Sse9I AATT 8 cut(s) 683, 818, 881, 924, 1382, 1442, 1582, 1788
SsiI CCGC 4 cut(s) 164, 1181, 1205, 1391
SspI AATATT 1 cut(s) 546
SspMI CTAG 1 cut(s) 107
StyD4I CCNGG 3 cut(s) 1103, 1596, 1683
StyI CCWWGG 5 cut(s) 81, 225, 458, 562, 1209
TaaI ACNGT 4 cut(s) 810, 858, 870, 1452
TaiI ACGT 1 cut(s) 247
TaqI TCGA 1 cut(s) 1651
TasI AATT 8 cut(s) 683, 818, 881, 924, 1382, 1442, 1582, 1788
TatI WGTACW 2 cut(s) 858, 1779
TauI GCSGC 2 cut(s) 1183, 1207
TfiI GAWTC 4 cut(s) 221, 1063, 1313, 1721
Tru1I TTAA 3 cut(s) 712, 947, 1810
Tru9I TTAA 3 cut(s) 712, 947, 1810
TscAI CASTG 2 cut(s) 1272, 1805
TseFI GTSAC 3 cut(s) 595, 1011, 1267
TseI GCWGC 9 cut(s) 343, 502, 664, 957, 1081, 1342, 1360, 1393, 1549
Tsp45I GTSAC 3 cut(s) 595, 1011, 1267
TspDTI ATGAA 5 cut(s) 146, 247, 680, 1467, 1546
TspGWI ACGGA 3 cut(s) 37, 78, 198
TspRI CASTG 2 cut(s) 1272, 1805
VpaK11BI GGWCC 1 cut(s) 1258
XagI CCTNNNNNAGG 1 cut(s) 1606
XapI RAATTY 1 cut(s) 1788
XceI RCATGY 1 cut(s) 122
XcmI CCANNNNNNNNNTGG 1 cut(s) 264
XspI CTAG 1 cut(s) 107
ZrmI AGTACT 1 cut(s) 860
Zsp2I ATGCAT 3 cut(s) 1038, 1254, 1828
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.