MD17G1215700.v1.1

Pyrophosphate-energized vacuolar membrane proton

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0000897
Physical Location & Seq
Forward (+)
26277687 .. 26283914
6228 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1215700.v1.1.491

Sequence Viewer

Length: 2304 bp
ATGGCAGTTCTGCTCTCAACCCTCGCGACGGAGATTGTGATCCCCGTCGCCGCCGTCGTGGGGATCGTGTTCTCTCTCGTCCAGTGGTTCCTGGTCTCGCTCGTGAAGGTCACGCCCGAGCGCAACGCGCCGCCTTCCGGCCCCAACAGTAACAAGAACGGCTACAATGACTACCTAATTGAGGAGGAGGAAGGCTTGAATGACCAGAACGTCGTCGTTAAGTGCGCTGAGATACAGAACGCTATCTCTGAAGGTTCAACATCCTTTCTTTTCACTATGTACCAGTATGTTGGGGTCTTCATGGTTGTATTTGCAATCCTGATCTTCCTCTTCCTCGGGTCTGTGGAGGGCTTCAGCACAAAGAGCCAGCCCTGCACCTATGATGCCGCAAAGACCTGCAAGCCAGCACTTGCAACTGCAATTTTCAGCACTGTAGCTTTTGTACTTGGTGGTATAACCTCTGTCCTGTCTGGTTTTCTTGGCATGAAAATTGCCACTTATGCCAATGCTAGGACAACATTGGAGGCCAGAAAAGGTGTTGGGAAGGCCTTTATTACTGCATTTAGGTCTGGTGCAGTTATGGGTTTCCTCCTTGCAGCAAATGGTCTTTTAGTACTTTTCATTACTATCAATCTCTTTAAGCTGTACTATGGTGATGACTGGGAAGGACTTTTTGAGTCCATTACTGGCTATGGTCTTGGTGGATCATCTATGGCTCTCTTCGGGAGAGTCGGTGGTGGTATCTATACCAAGGCTGCTGATGTTGGGGCTGACCTGGTAGGAAAGGTTGAAAGAAACATTCCTGAAGATGACCCAAGAAACCCAGCTGTGATTGCTGACAACGTTGGTGACAATGTTGGGGATATTGCTGGTATGGGATCTGATCTCTTTGGCTCATATGCCGAATCATCTTGTGCTGCTTTGGTTGTTGCTTCCATTTCCTCATTTGGAATCAACCATGAGTTCACCCCTATGTTGTATCCTCTCCTCATCAGTTCTGTGGGCATCATTGTTTGTTTAATCACAACCCTATTTGCCACTGATTTCTTTGAGATCAAGGCTGTCAAGGAAATTGAACCAGCTTTGAAAAAGCAGCTTATCATCTCCACTGGTCTCATGACTATTGGAATTGCAATTGTTAGCTGGATTGCTCTGCCATCATCCTTCACAATTTTCAATTTTGGAGTTCAGAAAGTTGTTAAAAACTGGCAATTGTTCTTGTGTGTGGCTGTTGGTCTTTGGGCTGGACTCATTATTGGGTTCGTCACCGAGTATTATACTAGCAACGCATACAGCCCTGTGCAAGATGTTGCTGACTCCTGCAGAACTGGTGCTGCCACCAATGTTATCTTTGGGCTTGCTCTGGGATACAAATCAGTCATCATTCCAATTTTTGCCATTGCAGTCAGCATTTATGTTAGTTTTAGCTTTGCTGCTATGTATGGCATTGCAGTGGCTGCACTTGGGATGCTGAGCACCATTGCTACAGGATTGGCCATTGATGCTTACGGTCCCATCAGTGACAATGCTGGAGGCATTGCTGAAATGGCTGGCATGAGCCATAGAATCCGTGAGAGAACTGATGCTCTTGATGCTGCTGGGAATACCACTGCTGCTATTGGAAAGGGATTTGCCATTGGATCTGCTGCTCTGGTGTCATTGGCTCTATTTGGCGCCTTTGTCAGTCGTGCAGCAATCTCAACTGTTGATGTCTTAACCCCAAAGGTCTTTATTGGACTTATCGTGGGCGCAATGCTTCCTTACTGGTTCTCTGCCATGACAATGAAGAGTGTGGGAAGTGCTGCACTAAAAATGGTTGAGGAAGTCCGTAGGCAGTTCAATACCATTCCAGGTCTCATGGAGGGCACTGCTAAGCCTGACTATGCTACCTGCGTCAAGATCTCCACTGATGCATCCATCAAGGAGATGATTCCACCTGGTGCCCTTGTCATTCTTACACCTCTTATTGTTGGAACATTCTTTGGCGTGGAGACCCTATCCGGTGTTCTGGCTGGTTCCCTTGTTTCTGGTGTGCAGATAGCGATATCTGCTTCTAACACTGGTGGTGCATGGGACAATGCTAAGAAGTACATTGAGGCCGGTGCTTCAGAGCATGCAAGGACCCTTGGCCCGAAGGGTTCAGACCCACACAAGGCAGCAGTGATTGGTGACACCATTGGAGACCCGCTCAAGGACACCTCAGGGCCATCACTCAACATCCTTATTAAGCTCATGGCAGTGGAGTCCCTCGTGTTTGCTCCCTTCTTCGCCACTCATGGCGGCCTACTTTTCAAGATCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 map00190
Pfam Domains
Protein Families
H_PPase PDH

Protein Analysis

768

Amino Acids

80.45

Weight (kDa)

5.25

Isoelectric Point (pI)

22.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
H_PPase PF03030 73 - 752 1.1e-252 Inorganic H+ pyrophosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 209
Acc36I ACCTGC 2 cut(s) 404, 1898
AccB1I GGYRCC 2 cut(s) 1673, 1940
AccBSI CCGCTC 1 cut(s) 2188
AccII CGCG 2 cut(s) 26, 128
AciI CCGC 5 cut(s) 51, 131, 387, 2186, 2280
AclI AACGTT 1 cut(s) 843
AclWI GGATC 5 cut(s) 34, 71, 712, 886, 1648
AcoI YGGCCR 1 cut(s) 1494
AcuI CTGAAG 4 cut(s) 270, 337, 825, 2091
AcyI GRCGYC 1 cut(s) 1674
AdeI CACNNNGTG 1 cut(s) 1940
AfaI GTAC 5 cut(s) 281, 444, 615, 647, 2090
AfiI CCNNNNNNNGG 7 cut(s) 28, 60, 137, 181, 510, 2152, 2191
AgsI TTSAA 8 cut(s) 199, 258, 791, 1076, 1087, 1177, 1840, 2293
AjnI CCWGG 4 cut(s) 90, 774, 1849, 1936
AluBI AGCT 8 cut(s) 437, 643, 827, 1082, 1096, 1143, 1428, 2230
AluI AGCT 8 cut(s) 437, 643, 827, 1082, 1096, 1143, 1428, 2230
Alw21I GWGCWC 1 cut(s) 1478
Alw26I GTCTC 5 cut(s) 100, 1118, 1859, 1985, 2175
AlwI GGATC 5 cut(s) 34, 71, 712, 886, 1648
AlwNI CAGNNNCTG 1 cut(s) 1457
Ama87I CYCGRG 2 cut(s) 116, 335
AoxI GGCC 8 cut(s) 139, 525, 546, 1494, 2097, 2128, 2204, 2281
AspLEI GCGC 5 cut(s) 123, 130, 227, 1676, 1751
AspS9I GGNCC 5 cut(s) 140, 1511, 2121, 2129, 2204
AsuHPI GGTGA 5 cut(s) 665, 860, 958, 1258, 2180
AvaI CYCGRG 2 cut(s) 116, 335
AvaII GGWCC 2 cut(s) 1511, 2121
AxyI CCTNAGG 1 cut(s) 2200
BaeGI GKGCMC 2 cut(s) 1868, 1945
BalI TGGCCA 1 cut(s) 1496
BanI GGYRCC 2 cut(s) 1673, 1940
BauI CACGAG 2 cut(s) 101, 2249
BbsI GAAGAC 1 cut(s) 289
Bbv12I GWGCWC 1 cut(s) 1478
BccI CCATC 4 cut(s) 1165, 1523, 1925, 2215
BceAI ACGGC 2 cut(s) 38, 175
BciT130I CCWGG 4 cut(s) 92, 776, 1851, 1938
BciVI GTATCC 2 cut(s) 990, 1361
BcoDI GTCTC 5 cut(s) 100, 1118, 1859, 1985, 2175
BfaI CTAG 2 cut(s) 510, 1281
BfmI CTRYAG 3 cut(s) 432, 1321, 1485
BfoI RGCGCY 1 cut(s) 1677
BfuAI ACCTGC 2 cut(s) 404, 1898
BfuI GTATCC 2 cut(s) 990, 1361
BglII AGATCT 2 cut(s) 1899, 2295
BlpI GCTNAGC 2 cut(s) 1472, 1872
BmcAI AGTACT 1 cut(s) 615
Bme1390I CCNGG 4 cut(s) 92, 776, 1851, 1938
Bme18I GGWCC 2 cut(s) 1511, 2121
BmeT110I CYCGRG 2 cut(s) 116, 335
BmgT120I GGNCC 5 cut(s) 140, 1511, 2121, 2129, 2204
BmiI GGNNCC 7 cut(s) 89, 142, 1513, 1675, 1942, 2017, 2123
BmrFI CCNGG 4 cut(s) 92, 776, 1851, 1938
BmrI ACTGGG 1 cut(s) 670
BmsI GCATC 8 cut(s) 373, 1014, 1458, 1492, 1573, 1582, 1900, 1922
BmuI ACTGGG 1 cut(s) 670
BpiI GAAGAC 1 cut(s) 289
BplI GAGNNNNNCTC 2 cut(s) 2172, 2204
BpmI CTGGAG 1 cut(s) 1551
Bpu1102I GCTNAGC 2 cut(s) 1472, 1872
BpuEI CTTGAG 1 cut(s) 2174
BsaBI GATNNNNATC 2 cut(s) 38, 1372
BsaHI GRCGYC 1 cut(s) 1674
BsaI GGTCTC 5 cut(s) 100, 1118, 1859, 1985, 2175
BsaJI CCNNGG 3 cut(s) 334, 750, 2125
BsaWI WCCGGW 1 cut(s) 2000
BsaXI ACNNNNNCTCC 2 cut(s) 718, 748
Bsc4I CCNNNNNNNGG 7 cut(s) 28, 60, 137, 181, 510, 2152, 2191
Bse118I RCCGGY 1 cut(s) 2099
Bse1I ACTGG 9 cut(s) 82, 283, 665, 691, 1114, 1211, 1333, 1769, 2065
Bse21I CCTNAGG 1 cut(s) 2200
Bse3DI GCAATG 5 cut(s) 1398, 1446, 1479, 1536, 1758
Bse8I GATNNNNATC 2 cut(s) 38, 1372
BseBI CCWGG 4 cut(s) 92, 776, 1851, 1938
BseDI CCNNGG 3 cut(s) 334, 750, 2125
BseGI GGATG 5 cut(s) 260, 1160, 1473, 1913, 2217
BseJI GATNNNNATC 2 cut(s) 38, 1372
BseLI CCNNNNNNNGG 7 cut(s) 28, 60, 137, 181, 510, 2152, 2191
BseMI GCAATG 5 cut(s) 1398, 1446, 1479, 1536, 1758
BseMII CTCAG 3 cut(s) 219, 1463, 2214
BseNI ACTGG 9 cut(s) 82, 283, 665, 691, 1114, 1211, 1333, 1769, 2065
BseRI GAGGAG 3 cut(s) 197, 200, 977
BseSI GKGCMC 2 cut(s) 1868, 1945
BseYI CCCAGC 2 cut(s) 823, 1598
BsgI GTGCAG 6 cut(s) 358, 594, 1443, 1710, 1788, 2054
Bsh1236I CGCG 2 cut(s) 26, 128
BshFI GGCC 8 cut(s) 141, 527, 548, 1496, 2099, 2130, 2206, 2283
BshNI GGYRCC 2 cut(s) 1673, 1940
BsiHKAI GWGCWC 1 cut(s) 1478
BsiHKCI CYCGRG 2 cut(s) 116, 335
BsiSI CCGG 3 cut(s) 138, 2001, 2100
BslFI GGGAC 3 cut(s) 1497, 2087, 2230
BslI CCNNNNNNNGG 7 cut(s) 28, 60, 137, 181, 510, 2152, 2191
BsmAI GTCTC 5 cut(s) 100, 1118, 1859, 1985, 2175
BsmFI GGGAC 3 cut(s) 1497, 2087, 2230
BsnI GGCC 8 cut(s) 141, 527, 548, 1496, 2099, 2130, 2206, 2283
Bso31I GGTCTC 5 cut(s) 100, 1118, 1859, 1985, 2175
BsoBI CYCGRG 2 cut(s) 116, 335
Bsp1286I GDGCHC 3 cut(s) 1478, 1868, 1945
Bsp1720I GCTNAGC 2 cut(s) 1472, 1872
Bsp68I TCGCGA 1 cut(s) 26
BspACI CCGC 5 cut(s) 51, 131, 387, 2186, 2280
BspANI GGCC 8 cut(s) 141, 527, 548, 1496, 2099, 2130, 2206, 2283
BspCNI CTCAG 3 cut(s) 220, 1464, 2213
BspFNI CGCG 2 cut(s) 26, 128
BspHI TCATGA 1 cut(s) 1116
BspLI GGNNCC 7 cut(s) 89, 142, 1513, 1675, 1942, 2017, 2123
BspMAI CTGCAG 1 cut(s) 1325
BspMI ACCTGC 2 cut(s) 404, 1898
BspPI GGATC 5 cut(s) 34, 71, 712, 886, 1648
BspT107I GGYRCC 2 cut(s) 1673, 1940
BspTNI GGTCTC 5 cut(s) 100, 1118, 1859, 1985, 2175
BsrBI CCGCTC 1 cut(s) 2188
BsrDI GCAATG 5 cut(s) 1398, 1446, 1479, 1536, 1758
BsrFI RCCGGY 1 cut(s) 2099
BsrI ACTGG 9 cut(s) 82, 283, 665, 691, 1114, 1211, 1333, 1769, 2065
BssAI RCCGGY 1 cut(s) 2099
BssECI CCNNGG 3 cut(s) 334, 750, 2125
BssNI GRCGYC 1 cut(s) 1674
BssSI CACGAG 2 cut(s) 101, 2249
BssT1I CCWWGG 2 cut(s) 750, 2125
Bst2BI CACGAG 2 cut(s) 101, 2249
Bst2UI CCWGG 4 cut(s) 92, 776, 1851, 1938
Bst4CI ACNGT 4 cut(s) 149, 433, 1511, 1705
Bst6I CTCTTC 3 cut(s) 335, 725, 1781
BstACI GRCGYC 1 cut(s) 1674
BstAPI GCANNNNNTGC 1 cut(s) 1457
BstC8I GCNNGC 6 cut(s) 368, 401, 405, 1359, 1552, 2115
BstDEI CTNAG 5 cut(s) 228, 1472, 1872, 2082, 2200
BstENI CCTNNNNNAGG 1 cut(s) 179
BstF5I GGATG 5 cut(s) 260, 1160, 1473, 1913, 2217
BstFNI CGCG 2 cut(s) 26, 128
BstH2I RGCGCY 1 cut(s) 1677
BstHHI GCGC 5 cut(s) 123, 130, 227, 1676, 1751
BstMAI GTCTC 5 cut(s) 100, 1118, 1859, 1985, 2175
BstNI CCWGG 4 cut(s) 92, 776, 1851, 1938
BstNSI RCATGY 1 cut(s) 2117
BstSCI CCNGG 4 cut(s) 90, 774, 1849, 1936
BstSFI CTRYAG 3 cut(s) 432, 1321, 1485
BstSLI GKGCMC 2 cut(s) 1868, 1945
BstUI CGCG 2 cut(s) 26, 128
BstV2I GAAGAC 1 cut(s) 289
BstX2I RGATCY 4 cut(s) 878, 1640, 1899, 2295
BstXI CCANNNNNNTGG 1 cut(s) 290
BstYI RGATCY 4 cut(s) 878, 1640, 1899, 2295
Bsu36I CCTNAGG 1 cut(s) 2200
BsuI GTATCC 2 cut(s) 990, 1361
BsuRI GGCC 8 cut(s) 141, 527, 548, 1496, 2099, 2130, 2206, 2283
BtsCI GGATG 5 cut(s) 260, 1160, 1473, 1913, 2217
BtsI GCAGTG 5 cut(s) 1458, 1608, 1866, 2166, 2244
BtuMI TCGCGA 1 cut(s) 26
BveI ACCTGC 2 cut(s) 404, 1898
Cac8I GCNNGC 6 cut(s) 368, 401, 405, 1359, 1552, 2115
CaiI CAGNNNCTG 1 cut(s) 1457
CciI TCATGA 1 cut(s) 1116
CfoI GCGC 5 cut(s) 123, 130, 227, 1676, 1751
Cfr10I RCCGGY 1 cut(s) 2099
Cfr13I GGNCC 5 cut(s) 140, 1511, 2121, 2129, 2204
CseI GACGC 1 cut(s) 1882
CsiI ACCWGGT 2 cut(s) 774, 1936
Csp6I GTAC 5 cut(s) 280, 443, 614, 646, 2089
CviQI GTAC 5 cut(s) 280, 443, 614, 646, 2089
DdeI CTNAG 5 cut(s) 228, 1472, 1872, 2082, 2200
DinI GGCGCC 1 cut(s) 1675
DraIII CACNNNGTG 1 cut(s) 1940
DrdI GACNNNNNNGTC 1 cut(s) 209
DseDI GACNNNNNNGTC 1 cut(s) 209
EaeI YGGCCR 1 cut(s) 1494
Eam1104I CTCTTC 3 cut(s) 335, 725, 1781
EarI CTCTTC 3 cut(s) 335, 725, 1781
Eco130I CCWWGG 2 cut(s) 750, 2125
Eco147I AGGCCT 1 cut(s) 548
Eco31I GGTCTC 5 cut(s) 100, 1118, 1859, 1985, 2175
Eco32I GATATC 1 cut(s) 2046
Eco47I GGWCC 2 cut(s) 1511, 2121
Eco57I CTGAAG 4 cut(s) 270, 337, 825, 2091
Eco81I CCTNAGG 1 cut(s) 2200
Eco88I CYCGRG 2 cut(s) 116, 335
EcoNI CCTNNNNNAGG 1 cut(s) 179
EcoO109I RGGNCCY 1 cut(s) 2121
EcoRII CCWGG 4 cut(s) 90, 774, 1849, 1936
EcoRV GATATC 1 cut(s) 2046
EcoT14I CCWWGG 2 cut(s) 750, 2125
EcoT22I ATGCAT 1 cut(s) 1915
EgeI GGCGCC 1 cut(s) 1675
EheI GGCGCC 1 cut(s) 1675
ErhI CCWWGG 2 cut(s) 750, 2125
FaqI GGGAC 3 cut(s) 1497, 2087, 2230
FauI CCCGC 1 cut(s) 2193
FauNDI CATATG 1 cut(s) 898
FokI GGATG 5 cut(s) 247, 1147, 1480, 1900, 2204
FspBI CTAG 2 cut(s) 510, 1281
GlaI GCGC 5 cut(s) 122, 129, 226, 1675, 1750
GsaI CCCAGC 2 cut(s) 827, 1602
GsuI CTGGAG 1 cut(s) 1551
HaeII RGCGCY 1 cut(s) 1677
HaeIII GGCC 8 cut(s) 141, 527, 548, 1496, 2099, 2130, 2206, 2283
HapII CCGG 3 cut(s) 138, 2001, 2100
HgaI GACGC 1 cut(s) 1882
HhaI GCGC 5 cut(s) 123, 130, 227, 1676, 1751
Hin1I GRCGYC 1 cut(s) 1674
Hin6I GCGC 5 cut(s) 121, 128, 225, 1674, 1749
HinP1I GCGC 5 cut(s) 121, 128, 225, 1674, 1749
HinfI GANTC 9 cut(s) 677, 729, 905, 951, 1248, 1316, 1566, 1930, 2243
HpaII CCGG 3 cut(s) 138, 2001, 2100
HphI GGTGA 5 cut(s) 665, 860, 958, 1258, 2180
Hpy166II GTNNAC 1 cut(s) 966
Hpy188I TCNGA 5 cut(s) 250, 883, 1191, 2110, 2143
Hpy188III TCNNGA 9 cut(s) 25, 103, 319, 724, 803, 1117, 1589, 1897, 2293
Hpy8I GTNNAC 1 cut(s) 966
Hpy99I CGWCG 5 cut(s) 31, 50, 59, 215, 218
HpyAV CCTTC 9 cut(s) 100, 144, 185, 245, 538, 659, 1174, 2128, 2272
HpyCH4III ACNGT 4 cut(s) 149, 433, 1511, 1705
HpyCH4IV ACGT 2 cut(s) 210, 843
HpyF3I CTNAG 5 cut(s) 228, 1472, 1872, 2082, 2200
HpySE526I ACGT 2 cut(s) 210, 843
Hsp92I GRCGYC 1 cut(s) 1674
HspAI GCGC 5 cut(s) 121, 128, 225, 1674, 1749
KasI GGCGCC 1 cut(s) 1673
LmnI GCTCC 1 cut(s) 2263
LweI GCATC 8 cut(s) 373, 1014, 1458, 1492, 1573, 1582, 1900, 1922
MabI ACCWGGT 2 cut(s) 774, 1936
MaeI CTAG 2 cut(s) 510, 1281
MaeII ACGT 2 cut(s) 210, 843
MaeIII GTNAC 6 cut(s) 109, 149, 848, 1264, 1520, 2168
MbiI CCGCTC 1 cut(s) 2188
MboII GAAGA 7 cut(s) 289, 316, 322, 712, 818, 1798, 2257
MfeI CAATTG 2 cut(s) 1134, 1211
MflI RGATCY 4 cut(s) 878, 1640, 1899, 2295
MhlI GDGCHC 3 cut(s) 1478, 1868, 1945
MlsI TGGCCA 1 cut(s) 1496
MluNI TGGCCA 1 cut(s) 1496
Mly113I GGCGCC 1 cut(s) 1674
MlyI GAGTC 5 cut(s) 686, 738, 1242, 1310, 2252
MmeI TCCRAC 1 cut(s) 1951
Mox20I TGGCCA 1 cut(s) 1496
Mph1103I ATGCAT 1 cut(s) 1915
MscI TGGCCA 1 cut(s) 1496
MseI TTAA 6 cut(s) 219, 639, 1019, 1200, 1715, 2226
MslI CAYNNNNRTG 4 cut(s) 971, 1451, 1781, 2237
Msp20I TGGCCA 1 cut(s) 1496
MspA1I CMGCKG 1 cut(s) 827
MspI CCGG 3 cut(s) 138, 2001, 2100
MspR9I CCNGG 4 cut(s) 92, 776, 1851, 1938
MunI CAATTG 2 cut(s) 1134, 1211
MvaI CCWGG 4 cut(s) 92, 776, 1851, 1938
MvnI CGCG 2 cut(s) 26, 128
NarI GGCGCC 1 cut(s) 1674
NdeI CATATG 1 cut(s) 898
NlaIV GGNNCC 7 cut(s) 89, 142, 1513, 1675, 1942, 2017, 2123
NmuCI GTSAC 5 cut(s) 109, 848, 1264, 1520, 2168
NruI TCGCGA 1 cut(s) 26
NsiI ATGCAT 1 cut(s) 1915
NspI RCATGY 1 cut(s) 2117
PaeI GCATGC 1 cut(s) 2117
PagI TCATGA 1 cut(s) 1116
PceI AGGCCT 1 cut(s) 548
PcsI WCGNNNNNNNCGW 2 cut(s) 54, 63
PfeI GAWTC 4 cut(s) 905, 951, 1566, 1930
PleI GAGTC 5 cut(s) 685, 737, 1242, 1310, 2251
PluTI GGCGCC 1 cut(s) 1677
PpsI GAGTC 5 cut(s) 685, 737, 1242, 1310, 2251
PpuMI RGGWCCY 1 cut(s) 2121
Psp1406I AACGTT 1 cut(s) 843
Psp5II RGGWCCY 1 cut(s) 2121
Psp6I CCWGG 4 cut(s) 90, 774, 1849, 1936
PspFI CCCAGC 2 cut(s) 823, 1598
PspGI CCWGG 4 cut(s) 90, 774, 1849, 1936
PspN4I GGNNCC 7 cut(s) 89, 142, 1513, 1675, 1942, 2017, 2123
PspPI GGNCC 5 cut(s) 140, 1511, 2121, 2129, 2204
PspPPI RGGWCCY 1 cut(s) 2121
PstI CTGCAG 1 cut(s) 1325
PstNI CAGNNNCTG 1 cut(s) 1457
PsuI RGATCY 4 cut(s) 878, 1640, 1899, 2295
PvuII CAGCTG 1 cut(s) 827
RruI TCGCGA 1 cut(s) 26
RsaI GTAC 5 cut(s) 281, 444, 615, 647, 2090
RsaNI GTAC 5 cut(s) 280, 443, 614, 646, 2089
RseI CAYNNNNRTG 4 cut(s) 971, 1451, 1781, 2237
SaqAI TTAA 6 cut(s) 219, 639, 1019, 1200, 1715, 2226
Sau96I GGNCC 5 cut(s) 140, 1511, 2121, 2129, 2204
ScaI AGTACT 1 cut(s) 615
SchI GAGTC 5 cut(s) 686, 738, 1242, 1310, 2252
ScrFI CCNGG 4 cut(s) 92, 776, 1851, 1938
SduI GDGCHC 3 cut(s) 1478, 1868, 1945
SexAI ACCWGGT 2 cut(s) 774, 1936
SfaNI GCATC 8 cut(s) 373, 1014, 1458, 1492, 1573, 1582, 1900, 1922
SfcI CTRYAG 3 cut(s) 432, 1321, 1485
SfoI GGCGCC 1 cut(s) 1675
SinI GGWCC 2 cut(s) 1511, 2121
SmiMI CAYNNNNRTG 4 cut(s) 971, 1451, 1781, 2237
SmlI CTYRAG 1 cut(s) 2189
SmoI CTYRAG 1 cut(s) 2189
SphI GCATGC 1 cut(s) 2117
SseBI AGGCCT 1 cut(s) 548
SsiI CCGC 5 cut(s) 51, 131, 387, 2186, 2280
SspDI GGCGCC 1 cut(s) 1673
SspMI CTAG 2 cut(s) 510, 1281
StuI AGGCCT 1 cut(s) 548
StyD4I CCNGG 4 cut(s) 90, 774, 1849, 1936
StyI CCWWGG 2 cut(s) 750, 2125
TaaI ACNGT 4 cut(s) 149, 433, 1511, 1705
TaiI ACGT 2 cut(s) 213, 846
TatI WGTACW 4 cut(s) 442, 613, 645, 2088
TauI GCSGC 4 cut(s) 53, 133, 389, 2283
TfiI GAWTC 4 cut(s) 905, 951, 1566, 1930
Tru1I TTAA 6 cut(s) 219, 639, 1019, 1200, 1715, 2226
Tru9I TTAA 6 cut(s) 219, 639, 1019, 1200, 1715, 2226
TseFI GTSAC 5 cut(s) 109, 848, 1264, 1520, 2168
Tsp45I GTSAC 5 cut(s) 109, 848, 1264, 1520, 2168
TspDTI ATGAA 4 cut(s) 289, 500, 610, 1799
TspGWI ACGGA 3 cut(s) 44, 1559, 1817
VpaK11BI GGWCC 2 cut(s) 1511, 2121
XagI CCTNNNNNAGG 1 cut(s) 179
XceI RCATGY 1 cut(s) 2117
XspI CTAG 2 cut(s) 510, 1281
ZrmI AGTACT 1 cut(s) 615
Zsp2I ATGCAT 1 cut(s) 1915
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.