Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0000897

Physical Location & Seq

Genomic Coordinates Forward (+)

28353246 .. 28357601

Gene Structure 4356 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1194100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2280 bp

ATGGGTTTGCTGAGTGAGGGACTGACTCAGGTGGTGATTCCTTTGGCTGCGGTTGTGGGGCTTGGTTTTGCTTTGCTGCAGTGGTTTTTGGTGTCAAAGGTGAAGGTCTCTGGCGGGCATGGTGAGCGAAATGGGTTCAAGGACAAGCTCATTGGAGATGAAGAAGAGGGTGTTAGCTCCGTTGAGGTTACCATCAAGTGTGCTGAGATTCAACATGCCATTTCCATAGGGGCGACCTCTTTTCTGTTCACCCAATACAGATACCTCAGCATTTTCGTCGGTGTTTTTAGCACCATAATCTTCCTCTTCCTTGGCTCAGTGAAGGGTTTTAGCACTAAAAGCGAAGCCTGCACATATAACACAGGCAATATGTGCAAACCGGCATTAGCCAACGCCTTCTTTAGTACTATTGCCTTCTTGCTTGGTGCTTTTACATCCGTCCTCTCTGGATTTCTCGGGATGAAGATCGCAACGTATGCCAATGCTAGAACAACTCTAGAAGCAAGGAGGGGTGTTGGGAAGGCTTTCATTACTGCCTTTCGCTCAGGTGCTGTGATGGGGTTCCTTCTTGCTGCCAATGGCCTATTGGTGCTGTATATCACTATCAATTTGTTTAAGTTGTATTATGGGGATGACTGGGAAGGACTATATGAGTCAATTACTGGTTATGGACTTGGAGGTTCTTCAATGGCACTCTTTGGGAGAGTTGGGGGAGGTATATACACGAAAGCTGCTGATGTTGGTGCTGATCTTGTCGGGAAAGTTGAACGAAATATCCCTGAAGATGATCCACGAAACCCCGCCGTTATTGCAGACAATGTGGGTGACAATGTAGGAGACATTGCCGGGATGGGATCTGACCTGTTTGGATCTTATGCCGAGTCTTCTTGTGCAGCATTGTTTGTTGCATCCATATCATCCTTTGGCATCGGTCATGACTACACCGCCATGTCGTATCCTTTGATCATAAGCTCAATGGGGATTGTGGTTTGTTTGGTAACAACCCTTTTCGCAACCGATCTGTTTGAAATCAAGAAAGTCAGTGAGATCGAACCCGCCTTGAAGCGTCAACTCCTCATCTCAACCGTCTTTATGACTGCTGGGATCGCCGCTGTCACTTTCGTATCTTTGCCATCAGAATTCACTCTCTTCAGCTTTGGAACCAATAAGGCTGTCAAAAACTGGTACCTCTTCTTTTGTGTATCAATCGGACTGTGGGCTGGTCTTGTTATTGGATACACCACAGAGTATTACACTAGCAATGCTTACAGTCCGGTGCAGGATGTGGCAGATTCATGCAGGACTGGTGCTGCAACAAACGTGATTTTCGGGCTGGCTCTTGGTTACAAATCAGTCATCATTCCTGTGTTCGCCATTGCGTTTGCTATCTACGTTAGCTTTAGCTTGGCTGCCATGTATGGAATAGCTGTGGCTGCTCTGGGAATGCTCAGCACTATCTCCACAGGTCTTGCAATTGATGCTTATGGCCCCATAAGCGACAATGCTGGTGGGATTGCAGAGATGGCCGGGATGAGCCACAAGATACGCGAAAGAACAGATGCTTTAGATGCTGCAGGAAACACAACTGCTGCTATTGGCAAGGGTTTTGCTATTGGATCAGCTGCTCTTGTTTCTCTTGCATTGTTTGGCGCCTATGTCAGCAGGGCGGGAATAGAGACCGTTGATGTGTTGACACCGAAAGTCTTCATTGGCTTGATTGTGGGAGCTATGCTTCCGTACTGGTTTTCCGCCATGACAATGAAGAGTGTGGGAAGTGCTGCACTCAAAATGGTCGAAGAGGTTCGCAGGCAGTTCAATACTATCCCGGGGCTCATGGAAGGAACAGCAAAGCCAGATTACGCAACTTGTGTCAAGATTTCTACCGATGCTTCCCTAAGGGAGATGATCCCACCAGGCGCCTTGGTCATGCTTACACCACTCGTTGCCGGTACCTTCTTTGGAGTCGAAACTCTTGCAGGGATTCTTGCAGGTTCACTTGTTTCCGGTGTGCAGATTGCCATTTCTGCATCCAACACCGGCGGGGCATGGGATAATGCAAAGAAATACATAGAGGCCGGCGGTTCAGAACATGCTAAATCACTAGGTCCGAAGGGTTCGGAACCCCACAAGGCGGCGGTCATAGGCGATACGATTGGAGATCCGCTCAAGGACACTTCAGGTCCTTCACTCAATATTCTGATCAAGCTCATGGCAGTTGAGTCCTTGGTGTTTGCTCCTTTCTTTGCAGCTCATGGAGGCCTCCTCTTCAAATGGCTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000325 GO:0001101 GO:0003674 GO:0003824 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005768 GO:0005773 GO:0005774 GO:0005794 GO:0005886 GO:0006810 GO:0006811 GO:0006950 GO:0006970 GO:0007275 GO:0008150 GO:0009414 GO:0009415 GO:0009507 GO:0009526 GO:0009536 GO:0009628 GO:0009651 GO:0009678 GO:0009705 GO:0009914 GO:0009926 GO:0009941 GO:0010008 GO:0010035 GO:0010248 GO:0010817 GO:0012505 GO:0015399 GO:0015405 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0022804 GO:0022857 GO:0031090 GO:0031410 GO:0031967 GO:0031975 GO:0031982 GO:0032501 GO:0032502 GO:0034220 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044433 GO:0044434 GO:0044435 GO:0044437 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0051179 GO:0051234 GO:0055085 GO:0060918 GO:0065007 GO:0065008 GO:0071944 GO:0097708 GO:0098588 GO:0098805 GO:0099402 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko00190 map00190

Pfam Domains

Protein Families

H_PPase

Protein Analysis

760

Amino Acids

79.57

Weight (kDa)

5.68

Isoelectric Point (pI)

23.16

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
H_PPase	PF03030	64 - 744	1.9e-253	Inorganic H+ pyrophosphatase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000897)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G15690 AT1G15690
fragaria_vesca	FvH4_1g02190 FvH4_1g02190 FvH4_1g02190 FvH4_6g07370 FvH4_6g07370 FvH4_6g07370 FvH4_6g07370 FvH4_6g07370 FvH4_6g29320 FvH4_6g29320 FvH4_6g29320 FvH4_6g29320 FvH4_6g29320 FvH4_6g29320
malus_domestica	MD02G1020700.v1.1 MD04G1194100.v1.1 MD12G1206600.v1.1 MD15G1163900.v1.1 MD17G1215700.v1.1
prunus_persica	Prupe.3G091900_v2.0.a1 Prupe.6G313800_v2.0.a1 Prupe.7G250800_v2.0.a1
pyrus_communis	pycom02g01810 pycom04g17150 pycom12g19440 pycom15g14710 pycom17g22030 pycom808g00300
rosa_chinensis	RchiOBHm_Chr2g0087281 RchiOBHm_Chr2g0134531 RchiOBHm_Chr3g0456971
rosa_laevigata	RLG00000015850 RLG00000019400 RLG00000025258
rosa_multiflora	Rmu_sc0004670.1_g000006 Rmu_sc0016008.1_g000005 Rmu_sc0017477.1_g000001 Rmu_sc0017478.1_g000001 Rmu_sc0017479.1_g000001 Rmu_sc0042014.1_g000001 Rmu_ssc0000438.1_g000050
rosa_roxburghii	Rroxscaffold_2G00110050 Rroxscaffold_2G00153750 Rroxscaffold_6G00422310
rosa_rugosa	Rorug01G0473600 Rorug02G0318400 Rorug03G0017900
rosa_samantha	Rh2AG027400 Rh2AG375000 Rh2AG375100 Rh2BG026600 Rh2BG376300 Rh2CG027700 Rh2CG354500 Rh2DG027300 Rh2DG393700 Rh3AG077800 Rh3BG079700 Rh3CG079600 Rh3DG081400
rosa_wichuraiana	Rw2G002150 Rw2G030190 Rw3G006670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1979
Acc65I	GGTACC	2 cut(s)	1185, 1949
AccB1I	GGYRCC	4 cut(s)	1185, 1649, 1916, 1949
AccBSI	CCGCTC	1 cut(s)	2164
AccII	CGCG	1 cut(s)	1548
AclWI	GGATC	7 cut(s)	782, 862, 877, 1112, 1624, 1900, 2153
AcoI	YGGCCR	1 cut(s)	1524
AcsI	RAATTY	1 cut(s)	1139
AcuI	CTGAAG	3 cut(s)	801, 1135, 2160
AcyI	GRCGYC	2 cut(s)	1650, 1917
AfaI	GTAC	4 cut(s)	406, 1187, 1739, 1951
AfiI	CCNNNNNNNGG	1 cut(s)	2131
AgsI	TTSAA	8 cut(s)	139, 212, 687, 767, 1028, 1063, 1816, 2269
AjnI	CCWGG	1 cut(s)	1912
AloI	GAACNNNNNNTCC	2 cut(s)	759, 791
Alw26I	GTCTC	3 cut(s)	112, 831, 1670
AlwI	GGATC	7 cut(s)	782, 862, 877, 1112, 1624, 1900, 2153
AlwNI	CAGNNNCTG	1 cut(s)	551
Ama87I	CYCGRG	2 cut(s)	455, 1825
AoxI	GGCC	5 cut(s)	580, 1486, 1524, 2073, 2257
ApoI	RAATTY	1 cut(s)	1139
Asp700I	GAANNNNTTC	3 cut(s)	524, 1703, 1800
Asp718I	GGTACC	2 cut(s)	1185, 1949
AspLEI	GCGC	2 cut(s)	1652, 1919
AspS9I	GGNCC	3 cut(s)	1487, 2105, 2180
AsuC2I	CCSGG	4 cut(s)	847, 1528, 1826, 1827
AsuHPI	GGTGA	5 cut(s)	46, 112, 134, 241, 836
AvaI	CYCGRG	2 cut(s)	455, 1825
AvaII	GGWCC	2 cut(s)	2105, 2180
AxyI	CCTNAGG	1 cut(s)	1895
BaeI	ACNNNNGTAYC	2 cut(s)	1107, 1140
BanI	GGYRCC	4 cut(s)	1185, 1649, 1916, 1949
BanII	GRGCYC	1 cut(s)	1833
BbsI	GAAGAC	2 cut(s)	876, 1696
BbvCI	CCTCAGC	1 cut(s)	266
BccI	CCATC	5 cut(s)	200, 550, 844, 1141, 1516
BceAI	ACGGC	1 cut(s)	788
BcgI	CGANNNNNNTGC	4 cut(s)	259, 293, 1955, 1989
BciT130I	CCWGG	1 cut(s)	1914
BciVI	GTATCC	2 cut(s)	966, 1229
BclI	TGATCA	2 cut(s)	963, 2199
BcnI	CCSGG	4 cut(s)	847, 1528, 1826, 1827
BcoDI	GTCTC	3 cut(s)	112, 831, 1670
BfaI	CTAG	4 cut(s)	486, 497, 1257, 2102
BfmI	CTRYAG	2 cut(s)	77, 1572
BfoI	RGCGCY	2 cut(s)	1653, 1920
BfuAI	ACCTGC	1 cut(s)	1979
BfuI	GTATCC	2 cut(s)	966, 1229
BlpI	GCTNAGC	1 cut(s)	1448
BmcAI	AGTACT	1 cut(s)	406
Bme1390I	CCNGG	5 cut(s)	847, 1528, 1826, 1827, 1914
Bme18I	GGWCC	2 cut(s)	2105, 2180
BmeT110I	CYCGRG	2 cut(s)	455, 1825
BmgT120I	GGNCC	3 cut(s)	1487, 2105, 2180
BmiI	GGNNCC	8 cut(s)	563, 1162, 1187, 1489, 1651, 1918, 1951, 2121
BmrFI	CCNGG	5 cut(s)	847, 1528, 1826, 1827, 1914
BmrI	ACTGGG	1 cut(s)	646
BmsI	GCATC	7 cut(s)	917, 936, 1468, 1549, 1558, 1876, 2036
BmuI	ACTGGG	1 cut(s)	646
BpiI	GAAGAC	2 cut(s)	876, 1696
BplI	GAGNNNNNCTC	4 cut(s)	2148, 2180, 2247, 2279
Bpu10I	CCTNAGC	2 cut(s)	266, 544
Bpu1102I	GCTNAGC	1 cut(s)	1448
BpuEI	CTTGAG	1 cut(s)	2150
BpuMI	CCSGG	4 cut(s)	847, 1528, 1826, 1827
BsaBI	GATNNNNATC	1 cut(s)	464
BsaHI	GRCGYC	2 cut(s)	1650, 1917
BsaI	GGTCTC	2 cut(s)	112, 1670
BsaJI	CCNNGG	5 cut(s)	310, 1825, 1826, 1920, 2223
BsaWI	WCCGGW	2 cut(s)	1273, 2003
Bsc4I	CCNNNNNNNGG	1 cut(s)	2131
Bse118I	RCCGGY	4 cut(s)	379, 1946, 2036, 2075
Bse1I	ACTGG	5 cut(s)	641, 667, 1187, 1309, 1745
Bse21I	CCTNAGG	1 cut(s)	1895
Bse3DI	GCAATG	3 cut(s)	840, 1267, 1374
Bse8I	GATNNNNATC	1 cut(s)	464
BseBI	CCWGG	1 cut(s)	1914
BseDI	CCNNGG	5 cut(s)	310, 1825, 1826, 1920, 2223
BseGI	GGATG	9 cut(s)	434, 465, 637, 855, 908, 917, 1288, 1536, 2027
BseJI	GATNNNNATC	1 cut(s)	464
BseLI	CCNNNNNNNGG	1 cut(s)	2131
BseMI	GCAATG	3 cut(s)	840, 1267, 1374
BseMII	CTCAG	6 cut(s)	41, 195, 280, 330, 558, 1462
BseNI	ACTGG	5 cut(s)	641, 667, 1187, 1309, 1745
BseRI	GAGGAG	2 cut(s)	1064, 2252
BseYI	CCCAGC	1 cut(s)	1100
BsgI	GTGCAG	5 cut(s)	334, 912, 1298, 1764, 2030
Bsh1236I	CGCG	1 cut(s)	1548
BshFI	GGCC	5 cut(s)	582, 1488, 1526, 2075, 2259
BshNI	GGYRCC	4 cut(s)	1185, 1649, 1916, 1949
BsiHKCI	CYCGRG	2 cut(s)	455, 1825
BsiSI	CCGG	9 cut(s)	380, 846, 1274, 1527, 1826, 1947, 2004, 2037, 2076
BslFI	GGGAC	1 cut(s)	33
BslI	CCNNNNNNNGG	1 cut(s)	2131
BsmAI	GTCTC	3 cut(s)	112, 831, 1670
BsmFI	GGGAC	1 cut(s)	33
BsmI	GAATGC	1 cut(s)	1449
BsnI	GGCC	5 cut(s)	582, 1488, 1526, 2075, 2259
Bso31I	GGTCTC	2 cut(s)	112, 1670
BsoBI	CYCGRG	2 cut(s)	455, 1825
Bsp1286I	GDGCHC	1 cut(s)	1833
Bsp1720I	GCTNAGC	1 cut(s)	1448
BspANI	GGCC	5 cut(s)	582, 1488, 1526, 2075, 2259
BspCNI	CTCAG	6 cut(s)	40, 196, 279, 329, 557, 1461
BspFNI	CGCG	1 cut(s)	1548
BspHI	TCATGA	1 cut(s)	934
BspLI	GGNNCC	8 cut(s)	563, 1162, 1187, 1489, 1651, 1918, 1951, 2121
BspMAI	CTGCAG	2 cut(s)	81, 1576
BspMI	ACCTGC	1 cut(s)	1979
BspPI	GGATC	7 cut(s)	782, 862, 877, 1112, 1624, 1900, 2153
BspT107I	GGYRCC	4 cut(s)	1185, 1649, 1916, 1949
BspTNI	GGTCTC	2 cut(s)	112, 1670
BsrBI	CCGCTC	1 cut(s)	2164
BsrDI	GCAATG	3 cut(s)	840, 1267, 1374
BsrFI	RCCGGY	4 cut(s)	379, 1946, 2036, 2075
BsrI	ACTGG	5 cut(s)	641, 667, 1187, 1309, 1745
BssAI	RCCGGY	4 cut(s)	379, 1946, 2036, 2075
BssECI	CCNNGG	5 cut(s)	310, 1825, 1826, 1920, 2223
BssNI	GRCGYC	2 cut(s)	1650, 1917
BssT1I	CCWWGG	3 cut(s)	310, 1920, 2223
Bst2UI	CCWGG	1 cut(s)	1914
Bst4CI	ACNGT	4 cut(s)	1087, 1215, 1271, 1681
Bst6I	CTCTTC	7 cut(s)	159, 311, 1154, 1196, 1757, 1791, 2270
BstACI	GRCGYC	2 cut(s)	1650, 1917
BstAPI	GCANNNNNTGC	3 cut(s)	372, 476, 1478
BstC8I	GCNNGC	5 cut(s)	116, 349, 1335, 1808, 2077
BstDEI	CTNAG	8 cut(s)	11, 27, 204, 266, 316, 544, 1448, 1895
BstEII	GGTNACC	1 cut(s)	187
BstF5I	GGATG	9 cut(s)	434, 465, 637, 855, 908, 917, 1288, 1536, 2027
BstFNI	CGCG	1 cut(s)	1548
BstH2I	RGCGCY	2 cut(s)	1653, 1920
BstHHI	GCGC	2 cut(s)	1652, 1919
BstMAI	GTCTC	3 cut(s)	112, 831, 1670
BstNI	CCWGG	1 cut(s)	1914
BstNSI	RCATGY	2 cut(s)	218, 2093
BstPI	GGTNACC	1 cut(s)	187
BstSCI	CCNGG	5 cut(s)	845, 1526, 1824, 1825, 1912
BstSFI	CTRYAG	2 cut(s)	77, 1572
BstUI	CGCG	1 cut(s)	1548
BstV2I	GAAGAC	2 cut(s)	876, 1696
BstX2I	RGATCY	3 cut(s)	854, 869, 2158
BstYI	RGATCY	3 cut(s)	854, 869, 2158
Bsu36I	CCTNAGG	1 cut(s)	1895
BsuI	GTATCC	2 cut(s)	966, 1229
BsuRI	GGCC	5 cut(s)	582, 1488, 1526, 2075, 2259
BtsCI	GGATG	9 cut(s)	434, 465, 637, 855, 908, 917, 1288, 1536, 2027
BtsI	GCAGTG	1 cut(s)	86
BtsIMutI	CAGTG	3 cut(s)	86, 324, 1048
BveI	ACCTGC	1 cut(s)	1979
Cac8I	GCNNGC	5 cut(s)	116, 349, 1335, 1808, 2077
CaiI	CAGNNNCTG	1 cut(s)	551
CciI	TCATGA	1 cut(s)	934
CfoI	GCGC	2 cut(s)	1652, 1919
Cfr10I	RCCGGY	4 cut(s)	379, 1946, 2036, 2075
Cfr13I	GGNCC	3 cut(s)	1487, 2105, 2180
Cfr9I	CCCGGG	1 cut(s)	1825
CseI	GACGC	1 cut(s)	1055
Csp6I	GTAC	4 cut(s)	405, 1186, 1738, 1950
CspCI	CAANNNNNGTGG	4 cut(s)	1450, 1485, 1489, 1524
CviQI	GTAC	4 cut(s)	405, 1186, 1738, 1950
DdeI	CTNAG	8 cut(s)	11, 27, 204, 266, 316, 544, 1448, 1895
DinI	GGCGCC	2 cut(s)	1651, 1918
EaeI	YGGCCR	1 cut(s)	1524
Eam1104I	CTCTTC	7 cut(s)	159, 311, 1154, 1196, 1757, 1791, 2270
EarI	CTCTTC	7 cut(s)	159, 311, 1154, 1196, 1757, 1791, 2270
EciI	GGCGGA	1 cut(s)	1738
Eco130I	CCWWGG	3 cut(s)	310, 1920, 2223
Eco147I	AGGCCT	1 cut(s)	2259
Eco24I	GRGCYC	1 cut(s)	1833
Eco31I	GGTCTC	2 cut(s)	112, 1670
Eco47I	GGWCC	2 cut(s)	2105, 2180
Eco57I	CTGAAG	3 cut(s)	801, 1135, 2160
Eco81I	CCTNAGG	1 cut(s)	1895
Eco88I	CYCGRG	2 cut(s)	455, 1825
Eco91I	GGTNACC	1 cut(s)	187
EcoO109I	RGGNCCY	1 cut(s)	2180
EcoO65I	GGTNACC	1 cut(s)	187
EcoRI	GAATTC	1 cut(s)	1139
EcoRII	CCWGG	1 cut(s)	1912
EcoT14I	CCWWGG	3 cut(s)	310, 1920, 2223
EcoT38I	GRGCYC	1 cut(s)	1833
EgeI	GGCGCC	2 cut(s)	1651, 1918
EheI	GGCGCC	2 cut(s)	1651, 1918
ErhI	CCWWGG	3 cut(s)	310, 1920, 2223
FaqI	GGGAC	1 cut(s)	33
FauI	CCCGC	5 cut(s)	107, 808, 1063, 1660, 2033
FbaI	TGATCA	2 cut(s)	963, 2199
FokI	GGATG	9 cut(s)	421, 472, 644, 862, 895, 904, 1295, 1543, 2014
FriOI	GRGCYC	1 cut(s)	1833
FspBI	CTAG	4 cut(s)	486, 497, 1257, 2102
GlaI	GCGC	2 cut(s)	1651, 1918
GsaI	CCCAGC	1 cut(s)	1104
HaeII	RGCGCY	2 cut(s)	1653, 1920
HaeIII	GGCC	5 cut(s)	582, 1488, 1526, 2075, 2259
HapII	CCGG	9 cut(s)	380, 846, 1274, 1527, 1826, 1947, 2004, 2037, 2076
HgaI	GACGC	1 cut(s)	1055
HhaI	GCGC	2 cut(s)	1652, 1919
Hin1I	GRCGYC	2 cut(s)	1650, 1917
Hin6I	GCGC	2 cut(s)	1650, 1917
HinP1I	GCGC	2 cut(s)	1650, 1917
HincII	GTYRAC	2 cut(s)	1070, 1692
HindII	GTYRAC	2 cut(s)	1070, 1692
HinfI	GANTC	9 cut(s)	25, 37, 208, 653, 881, 1292, 1962, 1981, 2219
HpaII	CCGG	9 cut(s)	380, 846, 1274, 1527, 1826, 1947, 2004, 2037, 2076
HphI	GGTGA	5 cut(s)	46, 112, 134, 241, 836
Hpy166II	GTNNAC	4 cut(s)	249, 1070, 1692, 1994
Hpy188I	TCNGA	7 cut(s)	859, 1138, 1211, 2086, 2109, 2119, 2199
Hpy188III	TCNNGA	7 cut(s)	447, 457, 497, 757, 935, 1033, 1873
Hpy8I	GTNNAC	4 cut(s)	249, 1070, 1692, 1994
Hpy99I	CGWCG	1 cut(s)	281
HpyCH4III	ACNGT	4 cut(s)	1087, 1215, 1271, 1681
HpyCH4IV	ACGT	3 cut(s)	473, 1320, 1392
HpyF3I	CTNAG	8 cut(s)	11, 27, 204, 266, 316, 544, 1448, 1895
HpySE526I	ACGT	3 cut(s)	473, 1320, 1392
Hsp92I	GRCGYC	2 cut(s)	1650, 1917
HspAI	GCGC	2 cut(s)	1650, 1917
KasI	GGCGCC	2 cut(s)	1649, 1916
KpnI	GGTACC	2 cut(s)	1189, 1953
KroI	GCCGGC	1 cut(s)	2075
KroNI	GCCGGC	1 cut(s)	2077
Ksp22I	TGATCA	2 cut(s)	963, 2199
LmnI	GCTCC	3 cut(s)	182, 1724, 2239
LweI	GCATC	7 cut(s)	917, 936, 1468, 1549, 1558, 1876, 2036
MaeI	CTAG	4 cut(s)	486, 497, 1257, 2102
MaeII	ACGT	3 cut(s)	473, 1320, 1392
MaeIII	GTNAC	5 cut(s)	187, 824, 997, 1114, 1343
MbiI	CCGCTC	1 cut(s)	2164
MfeI	CAATTG	1 cut(s)	1473
MflI	RGATCY	3 cut(s)	854, 869, 2158
MhlI	GDGCHC	1 cut(s)	1833
MluCI	AATT	4 cut(s)	607, 657, 1139, 1473
Mly113I	GGCGCC	2 cut(s)	1650, 1917
MlyI	GAGTC	5 cut(s)	19, 662, 890, 1971, 2228
MmeI	TCCRAC	1 cut(s)	2055
MroNI	GCCGGC	1 cut(s)	2075
MroXI	GAANNNNTTC	3 cut(s)	524, 1703, 1800
MseI	TTAA	1 cut(s)	615
MslI	CAYNNNNRTG	3 cut(s)	947, 1364, 1757
MspA1I	CMGCKG	2 cut(s)	1112, 1622
MspI	CCGG	9 cut(s)	380, 846, 1274, 1527, 1826, 1947, 2004, 2037, 2076
MspR9I	CCNGG	5 cut(s)	847, 1528, 1826, 1827, 1914
MunI	CAATTG	1 cut(s)	1473
Mva1269I	GAATGC	1 cut(s)	1449
MvaI	CCWGG	1 cut(s)	1914
MvnI	CGCG	1 cut(s)	1548
NaeI	GCCGGC	1 cut(s)	2077
NarI	GGCGCC	2 cut(s)	1650, 1917
NciI	CCSGG	4 cut(s)	847, 1528, 1826, 1827
NgoMIV	GCCGGC	1 cut(s)	2075
NlaIV	GGNNCC	8 cut(s)	563, 1162, 1187, 1489, 1651, 1918, 1951, 2121
NmeAIII	GCCGAG	1 cut(s)	904
NmuCI	GTSAC	2 cut(s)	824, 1114
NspI	RCATGY	2 cut(s)	218, 2093
PagI	TCATGA	1 cut(s)	934
PceI	AGGCCT	1 cut(s)	2259
PctI	GAATGC	1 cut(s)	1449
PdiI	GCCGGC	1 cut(s)	2077
PdmI	GAANNNNTTC	3 cut(s)	524, 1703, 1800
PfeI	GAWTC	4 cut(s)	37, 208, 1292, 1981
PleI	GAGTC	5 cut(s)	19, 661, 889, 1970, 2227
PluTI	GGCGCC	2 cut(s)	1653, 1920
PpsI	GAGTC	5 cut(s)	19, 661, 889, 1970, 2227
PpuMI	RGGWCCY	1 cut(s)	2180
Psp5II	RGGWCCY	1 cut(s)	2180
Psp6I	CCWGG	1 cut(s)	1912
PspEI	GGTNACC	1 cut(s)	187
PspFI	CCCAGC	1 cut(s)	1100
PspGI	CCWGG	1 cut(s)	1912
PspN4I	GGNNCC	8 cut(s)	563, 1162, 1187, 1489, 1651, 1918, 1951, 2121
PspPI	GGNCC	3 cut(s)	1487, 2105, 2180
PspPPI	RGGWCCY	1 cut(s)	2180
PstI	CTGCAG	2 cut(s)	81, 1576
PstNI	CAGNNNCTG	1 cut(s)	551
PsuI	RGATCY	3 cut(s)	854, 869, 2158
PvuII	CAGCTG	1 cut(s)	1622
RsaI	GTAC	4 cut(s)	406, 1187, 1739, 1951
RsaNI	GTAC	4 cut(s)	405, 1186, 1738, 1950
RseI	CAYNNNNRTG	3 cut(s)	947, 1364, 1757
SaqAI	TTAA	1 cut(s)	615
Sau96I	GGNCC	3 cut(s)	1487, 2105, 2180
ScaI	AGTACT	1 cut(s)	406
SchI	GAGTC	5 cut(s)	19, 662, 890, 1971, 2228
ScrFI	CCNGG	5 cut(s)	847, 1528, 1826, 1827, 1914
SduI	GDGCHC	1 cut(s)	1833
SfaNI	GCATC	7 cut(s)	917, 936, 1468, 1549, 1558, 1876, 2036
SfcI	CTRYAG	2 cut(s)	77, 1572
SfoI	GGCGCC	2 cut(s)	1651, 1918
SgrAI	CRCCGGYG	1 cut(s)	2036
SinI	GGWCC	2 cut(s)	2105, 2180
SmaI	CCCGGG	1 cut(s)	1827
SmiMI	CAYNNNNRTG	3 cut(s)	947, 1364, 1757
SmlI	CTYRAG	1 cut(s)	2165
SmoI	CTYRAG	1 cut(s)	2165
Sse9I	AATT	4 cut(s)	607, 657, 1139, 1473
SseBI	AGGCCT	1 cut(s)	2259
SspDI	GGCGCC	2 cut(s)	1649, 1916
SspI	AATATT	1 cut(s)	2194
SspMI	CTAG	4 cut(s)	486, 497, 1257, 2102
StuI	AGGCCT	1 cut(s)	2259
StyD4I	CCNGG	5 cut(s)	845, 1526, 1824, 1825, 1912
StyI	CCWWGG	3 cut(s)	310, 1920, 2223
TaaI	ACNGT	4 cut(s)	1087, 1215, 1271, 1681
TaiI	ACGT	3 cut(s)	476, 1323, 1395
TaqI	TCGA	3 cut(s)	1050, 1794, 1965
TaqII	GACCGA	1 cut(s)	920
TasI	AATT	4 cut(s)	607, 657, 1139, 1473
TatI	WGTACW	1 cut(s)	404
TauI	GCSGC	2 cut(s)	1112, 2135
TfiI	GAWTC	4 cut(s)	37, 208, 1292, 1981
Tru1I	TTAA	1 cut(s)	615
Tru9I	TTAA	1 cut(s)	615
TscAI	CASTG	3 cut(s)	86, 324, 1048
TseFI	GTSAC	2 cut(s)	824, 1114
Tsp45I	GTSAC	2 cut(s)	824, 1114
TspDTI	ATGAA	6 cut(s)	174, 476, 517, 1284, 1696, 1775
TspGWI	ACGGA	3 cut(s)	169, 427, 1725
TspMI	CCCGGG	1 cut(s)	1825
TspRI	CASTG	3 cut(s)	86, 324, 1048
VpaK11BI	GGWCC	2 cut(s)	2105, 2180
XapI	RAATTY	1 cut(s)	1139
XbaI	TCTAGA	1 cut(s)	496
XceI	RCATGY	2 cut(s)	218, 2093
XcmI	CCANNNNNNNNNTGG	1 cut(s)	583
XmaI	CCCGGG	1 cut(s)	1825
XmnI	GAANNNNTTC	3 cut(s)	524, 1703, 1800
XspI	CTAG	4 cut(s)	486, 497, 1257, 2102
ZrmI	AGTACT	1 cut(s)	406

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.