pycom15g14710

Pyrophosphate-energized vacuolar membrane proton

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0000897
Physical Location & Seq
Forward (+)
10080148 .. 10084135
3988 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g14710.6

Sequence Viewer

Length: 2529 bp
ATGATTTTTAATTATAAAACCCAATCTCCATCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCACTGATATAAACCCACTCTTAACCATCCACAGACACACTCTAGTTCACACTCTCTCTTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCTGAACTCCAAGCCTTCCTCTGTTTCTCTGTGTTTCCTCTTTTGGGCAAAGGAGCTATGGGGGCGACGATACTCCCAGATCTCGGGGCGGAGATTTTGATTCCGCTGTGCGCGGTGATCGGCATCGCCTTCTCCTTGGTCCAGTGGATGTACGTTTCGCAGGTTAAGCTCTCCCCAGGCGGTGGCCGGGACTCCAATTCCGGCGGCGCTGGCAAAAATGGGTGCAACGATTACCTCATCGAGGAAGAAGAAGGCGGTAACGATCACAACGTCGTCCTCAGATGCGCCGAAATCCAAACCGCCATTTCCGAAGGAGCAACGTCGTTCCTTTTCACGGAGTACAAGTATGTGGGTGCTTTCATGGTCGCTTTTGCAATCTTGATCTTCCTTTTCCTTGGGTCCGTGGAGGGATTTAGCACAAGTGGCCGTGCGTGTACGTATGACCAAACCAAGATATGCAAACCGGCTCTCGCCACCGCCATCTTCAGCACCGTTTCCTTCTTGCTTGGTGCCATAACTTCAGTGGTTTCGGGATTCCTCGGGATGAAGATTGCTACTTACGCAAATGCCAGAACAACGTTGGAGGCAAGAAAAGGTGTTGGGAAGGCTTTTATTACTGCATTTAGATCAGGAGCTGTGATGGGATTTCTCCTCGCTTCAAGTGGTCTGTTGGTTCTTTACATTGCTATCAATCTCTTCAAGTTGTACTACGGTGATGATTGGGGTGGTCTTTTCGAGGCTATAACTGGCTATGGTCTTGGTGGATCTTCCATGGCTTTATTTGGCAGAGTTGGTGGAGGCATCTATACCAAAGCTGCTGATGTCGGTGCTGATCTTGTCGGCAAGGTTGAAAGAAATATTCCCGAGGATGACCCAAGGAACCCGGCTGTGATAGCTGATAATGTCGGAGACAATGTTGGAGATATAGCTGGCATGGGATCTGATCTTTTTGGATCCTATGCTGAGTCATCGTGTGCTGCCCTTGTGGTTGCTTCCATTTCCTCATTTGGGATCAATCACGAGTTAACTGCAATGCTGTATCCTCTCATTGTCAGTTCCATCGGCATCCTTGTTTGTCTGCTCACCACCCTATTTGCAACCGATTTCTTTGAGATCAAGGCTGTAAAGGAAATTGAGCCAGCATTGAAGAAGCAGCTCATCATCTCTACTGTACTCATGACTGTTGGAGTTGCGATTGCTACATGGATTTCTGTTCCATCTTCCTTCACAATCTTCAATTTCGGAACACAGAAAGTTGTGAAGAGCTGGCAGCTATTCTTATGCGTCGGTGTTGGTTTGTGGGCTGGGCTTATCATTGGGTTCGTAACAGAGTACTATACAAGCAATGCGTACAGCCCTGTGCAAGATGTTGCTGATTCTTGCCGGACTGGAGCTGCGACCAATGTCATTTTTGGCCTTGCATTGGGATACAAATCCGTCATTATTCCTATATTTGCCATTGCAGTCAGCATTTTTGTTAGTTTTAGCTTTGCAGCTATGTACGGGATTGCCGTAGCTGCCCTTGGAATGCTGAGCACCATTGCAACTGGATTAGCCATTGATGCATATGGTCCCATCAGTGACAATGCTGGAGGCATTGCTGAGATGGCAGGCATGAGCCACAGAATCAGAGAGAGAACTGATGCTCTTGATGCTGCAGGAAACACCACTGCCGCTATTGGAAAGGGATTTGCAATTGGCTCTGCTGCTCTTGTGTCCCTTGCTCTATTTGGTGCCTTTGTCAGCCGTGCTGGTATCGAAACGGTTGATGTGTTGACCCCAAAAGTGATTATTGGTTTGTTAGTGGGCGCAATGCTTCCGTATTGGTTCTCTGCCATGACAATGAAGAGTGTGGGAAGTGCAGCTTTAAAGATGGTTGAGGAAGTGCGCAGGCAATTCAATACCATTCCAGGTCTCATGGAGGGCACTGCCAAGCCGGACTACGCTACATGTGTTAAGATTTCGACTGATGCTTCCATCAAAGAAATGATCCCACCTGGTGCCCTTGTCATGCTTACACCCCTCATTGTTGGGATCTTTTTTGGTGTGGAAACTCTCGCTGGCGTGCTTGCTGGATCCCTTGTCTCCGGTGTACAGATTGCCATTTCTGCATCCAACACGGGCGGTGCCTGGGATAATGCCAAGAAATACATTGAGGCTGGTGCTTCGGAGCATGCAAGGACCCTTGGTCCAAAAGGATCAGAATGCCACAAAGCTGCAGTCATTGGCGACACCATTGGAGACCCTCTCAAGGATACGTCTGGACCATCGCTCAACATTCTTATCAAGCTTATGGCCGTTGAGTCTCTTGTGTTTGCTCCGTTCTTTGCCACACACGGTGGGCTGCTATTCAAGATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 map00190
Pfam Domains
Protein Families
H_PPase

Protein Analysis

843

Amino Acids

87.98

Weight (kDa)

5.53

Isoelectric Point (pI)

27.07

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 15
Acc16I TGCGCA 1 cut(s) 2057
Acc36I ACCTGC 1 cut(s) 319
AccB1I GGYRCC 4 cut(s) 677, 1901, 2168, 2294
AccB7I CCANNNNNTGG 1 cut(s) 350
AccII CGCG 1 cut(s) 281
AclI AACGTT 1 cut(s) 746
AcoI YGGCCR 3 cut(s) 352, 592, 2463
AcuI CTGAAG 2 cut(s) 637, 672
AdeI CACNNNGTG 2 cut(s) 2168, 2507
AfaI GTAC 9 cut(s) 320, 509, 604, 875, 1341, 1502, 1520, 1670, 2262
AfiI CCNNNNNNNGG 8 cut(s) 212, 251, 350, 409, 502, 1176, 1591, 2419
AflIII ACRYGT 1 cut(s) 2117
AgsI TTSAA 7 cut(s) 828, 868, 1019, 1315, 1405, 2068, 2521
AhdI GACNNNNNGTC 1 cut(s) 2355
AjnI CCWGG 4 cut(s) 343, 2077, 2164, 2297
Alw21I GWGCWC 1 cut(s) 1706
Alw26I GTCTC 5 cut(s) 1071, 2087, 2257, 2403, 2478
AlwNI CAGNNNCTG 1 cut(s) 803
Ama87I CYCGRG 3 cut(s) 251, 707, 1031
AoxI GGCC 4 cut(s) 352, 592, 1582, 2463
ArsI GACNNNNNNTTYG 2 cut(s) 2373, 2405
AspLEI GCGC 5 cut(s) 281, 377, 455, 1979, 2058
AspS9I GGNCC 6 cut(s) 307, 567, 1739, 2349, 2357, 2432
AsuC2I CCSGG 2 cut(s) 356, 1052
AsuHPI GGTGA 3 cut(s) 295, 893, 1243
AvaI CYCGRG 3 cut(s) 251, 707, 1031
AvaII GGWCC 6 cut(s) 307, 567, 1739, 2349, 2357, 2432
BaeGI GKGCMC 2 cut(s) 2096, 2173
BamHI GGATCC 2 cut(s) 1121, 2243
BanI GGYRCC 4 cut(s) 677, 1901, 2168, 2294
BauI CACGAG 1 cut(s) 1187
Bbv12I GWGCWC 1 cut(s) 1706
BceAI ACGGC 4 cut(s) 579, 1664, 1899, 2450
BcgI CGANNNNNNTGC 2 cut(s) 1178, 1212
BciT130I CCWGG 4 cut(s) 345, 2079, 2166, 2299
BciVI GTATCC 3 cut(s) 1218, 1589, 2416
BcnI CCSGG 2 cut(s) 356, 1052
BcoDI GTCTC 5 cut(s) 1071, 2087, 2257, 2403, 2478
BfaI CTAG 1 cut(s) 119
BfmI CTRYAG 2 cut(s) 1824, 2385
BfoI RGCGCY 1 cut(s) 378
BfuAI ACCTGC 1 cut(s) 319
BfuI GTATCC 3 cut(s) 1218, 1589, 2416
BglII AGATCT 1 cut(s) 247
BlpI GCTNAGC 1 cut(s) 1700
BmcAI AGTACT 1 cut(s) 1502
Bme1390I CCNGG 6 cut(s) 345, 356, 1052, 2079, 2166, 2299
Bme18I GGWCC 6 cut(s) 307, 567, 1739, 2349, 2357, 2432
BmeRI GACNNNNNGTC 1 cut(s) 2355
BmeT110I CYCGRG 3 cut(s) 251, 707, 1031
BmgT120I GGNCC 6 cut(s) 307, 567, 1739, 2349, 2357, 2432
BmrFI CCNGG 6 cut(s) 345, 356, 1052, 2079, 2166, 2299
BmsI GCATC 9 cut(s) 300, 440, 978, 1242, 1720, 1801, 1810, 2128, 2288
BoxI GACNNNNGTC 1 cut(s) 1571
BplI GAGNNNNNCTC 2 cut(s) 2400, 2432
BpmI CTGGAG 2 cut(s) 1578, 1779
Bpu1102I GCTNAGC 1 cut(s) 1700
BpuEI CTTGAG 1 cut(s) 2402
BpuMI CCSGG 2 cut(s) 356, 1052
BsaAI YACGTR 1 cut(s) 606
BsaBI GATNNNNATC 2 cut(s) 290, 1600
BsaI GGTCTC 2 cut(s) 2087, 2403
BsaWI WCCGGW 1 cut(s) 2255
BsaXI ACNNNNNCTCC 6 cut(s) 10, 40, 497, 527, 1068, 1098
Bsc4I CCNNNNNNNGG 8 cut(s) 212, 251, 350, 409, 502, 1176, 1591, 2419
Bse118I RCCGGY 1 cut(s) 631
Bse1I ACTGG 4 cut(s) 310, 919, 1561, 1720
Bse3DI GCAATG 7 cut(s) 849, 1206, 1519, 1626, 1707, 1764, 1986
Bse8I GATNNNNATC 2 cut(s) 290, 1600
BseBI CCWGG 4 cut(s) 345, 2079, 2166, 2299
BseGI GGATG 6 cut(s) 102, 321, 717, 1042, 1233, 2279
BseJI GATNNNNATC 2 cut(s) 290, 1600
BseLI CCNNNNNNNGG 8 cut(s) 212, 251, 350, 409, 502, 1176, 1591, 2419
BseMI GCAATG 7 cut(s) 849, 1206, 1519, 1626, 1707, 1764, 1986
BseMII CTCAG 4 cut(s) 460, 1122, 1691, 1761
BseNI ACTGG 4 cut(s) 310, 919, 1561, 1720
BseRI GAGGAG 1 cut(s) 809
BseSI GKGCMC 2 cut(s) 2096, 2173
BseYI CCCAGC 1 cut(s) 1472
BsgI GTGCAG 1 cut(s) 2049
Bsh1236I CGCG 1 cut(s) 281
BshFI GGCC 4 cut(s) 354, 594, 1584, 2465
BshNI GGYRCC 4 cut(s) 677, 1901, 2168, 2294
BsiHKAI GWGCWC 1 cut(s) 1706
BsiHKCI CYCGRG 3 cut(s) 251, 707, 1031
BsiSI CCGG 7 cut(s) 355, 369, 632, 1052, 1552, 2105, 2256
BslFI GGGAC 3 cut(s) 371, 1725, 1870
BslI CCNNNNNNNGG 8 cut(s) 212, 251, 350, 409, 502, 1176, 1591, 2419
BsmAI GTCTC 5 cut(s) 1071, 2087, 2257, 2403, 2478
BsmFI GGGAC 3 cut(s) 371, 1725, 1870
BsmI GAATGC 2 cut(s) 1701, 2378
BsnI GGCC 4 cut(s) 354, 594, 1584, 2465
Bso31I GGTCTC 2 cut(s) 2087, 2403
BsoBI CYCGRG 3 cut(s) 251, 707, 1031
Bsp1286I GDGCHC 3 cut(s) 1706, 2096, 2173
Bsp1407I TGTACA 1 cut(s) 2260
Bsp1720I GCTNAGC 1 cut(s) 1700
Bsp19I CCATGG 1 cut(s) 939
BspANI GGCC 4 cut(s) 354, 594, 1584, 2465
BspCNI CTCAG 4 cut(s) 459, 1123, 1692, 1762
BspFNI CGCG 1 cut(s) 281
BspHI TCATGA 1 cut(s) 1344
BspMAI CTGCAG 2 cut(s) 1828, 2389
BspMI ACCTGC 1 cut(s) 319
BspQI GCTCTTC 1 cut(s) 1424
BspT107I GGYRCC 4 cut(s) 677, 1901, 2168, 2294
BspTNI GGTCTC 2 cut(s) 2087, 2403
BsrDI GCAATG 7 cut(s) 849, 1206, 1519, 1626, 1707, 1764, 1986
BsrFI RCCGGY 1 cut(s) 631
BsrGI TGTACA 1 cut(s) 2260
BsrI ACTGG 4 cut(s) 310, 919, 1561, 1720
BssAI RCCGGY 1 cut(s) 631
BssSI CACGAG 1 cut(s) 1187
BssT1I CCWWGG 6 cut(s) 303, 562, 939, 1043, 1690, 2353
Bst2BI CACGAG 1 cut(s) 1187
Bst2UI CCWGG 4 cut(s) 345, 2079, 2166, 2299
Bst4CI ACNGT 6 cut(s) 661, 881, 1339, 1351, 1933, 2507
Bst6I CTCTTC 4 cut(s) 139, 869, 1424, 2009
BstAUI TGTACA 1 cut(s) 2260
BstBAI YACGTR 1 cut(s) 606
BstDEI CTNAG 4 cut(s) 446, 1131, 1700, 1770
BstDSI CCRYGG 2 cut(s) 570, 939
BstENI CCTNNNNNAGG 1 cut(s) 407
BstF5I GGATG 6 cut(s) 102, 321, 717, 1042, 1233, 2279
BstFNI CGCG 1 cut(s) 281
BstH2I RGCGCY 1 cut(s) 378
BstHHI GCGC 5 cut(s) 281, 377, 455, 1979, 2058
BstMAI GTCTC 5 cut(s) 1071, 2087, 2257, 2403, 2478
BstNI CCWGG 4 cut(s) 345, 2079, 2166, 2299
BstNSI RCATGY 2 cut(s) 2121, 2345
BstPAI GACNNNNGTC 1 cut(s) 1571
BstSCI CCNGG 6 cut(s) 343, 354, 1050, 2077, 2164, 2297
BstSFI CTRYAG 2 cut(s) 1824, 2385
BstSLI GKGCMC 2 cut(s) 2096, 2173
BstSNI TACGTA 1 cut(s) 606
BstUI CGCG 1 cut(s) 281
BstX2I RGATCY 6 cut(s) 247, 932, 1106, 1121, 2202, 2243
BstYI RGATCY 6 cut(s) 247, 932, 1106, 1121, 2202, 2243
BsuI GTATCC 3 cut(s) 1218, 1589, 2416
BsuRI GGCC 4 cut(s) 354, 594, 1584, 2465
BtgI CCRYGG 2 cut(s) 570, 939
BtgZI GCGATG 2 cut(s) 277, 2421
BtsCI GGATG 6 cut(s) 102, 321, 717, 1042, 1233, 2279
BtsI GCAGTG 2 cut(s) 1836, 2094
BtsIMutI CAGTG 6 cut(s) 78, 317, 696, 1753, 1836, 2094
BveI ACCTGC 1 cut(s) 319
CaiI CAGNNNCTG 1 cut(s) 803
CciI TCATGA 1 cut(s) 1344
CfoI GCGC 5 cut(s) 281, 377, 455, 1979, 2058
Cfr10I RCCGGY 1 cut(s) 631
Cfr13I GGNCC 6 cut(s) 307, 567, 1739, 2349, 2357, 2432
CseI GACGC 1 cut(s) 1441
CsiI ACCWGGT 1 cut(s) 2164
Csp6I GTAC 9 cut(s) 319, 508, 603, 874, 1340, 1501, 1519, 1669, 2261
CspCI CAANNNNNGTGG 2 cut(s) 1243, 1278
CviQI GTAC 9 cut(s) 319, 508, 603, 874, 1340, 1501, 1519, 1669, 2261
DdeI CTNAG 4 cut(s) 446, 1131, 1700, 1770
DraI TTTAAA 1 cut(s) 2037
DraIII CACNNNGTG 2 cut(s) 2168, 2507
DriI GACNNNNNGTC 1 cut(s) 2355
EaeI YGGCCR 3 cut(s) 352, 592, 2463
Eam1104I CTCTTC 4 cut(s) 139, 869, 1424, 2009
Eam1105I GACNNNNNGTC 1 cut(s) 2355
EarI CTCTTC 4 cut(s) 139, 869, 1424, 2009
EciI GGCGGA 1 cut(s) 272
Eco105I TACGTA 1 cut(s) 606
Eco130I CCWWGG 6 cut(s) 303, 562, 939, 1043, 1690, 2353
Eco31I GGTCTC 2 cut(s) 2087, 2403
Eco47I GGWCC 6 cut(s) 307, 567, 1739, 2349, 2357, 2432
Eco57I CTGAAG 2 cut(s) 637, 672
Eco88I CYCGRG 3 cut(s) 251, 707, 1031
EcoNI CCTNNNNNAGG 1 cut(s) 407
EcoO109I RGGNCCY 1 cut(s) 2349
EcoRII CCWGG 4 cut(s) 343, 2077, 2164, 2297
EcoT14I CCWWGG 6 cut(s) 303, 562, 939, 1043, 1690, 2353
EcoT22I ATGCAT 1 cut(s) 1735
ErhI CCWWGG 6 cut(s) 303, 562, 939, 1043, 1690, 2353
FalI AAGNNNNNCTT 2 cut(s) 2017, 2049
FaqI GGGAC 3 cut(s) 371, 1725, 1870
FauNDI CATATG 1 cut(s) 1735
FokI GGATG 6 cut(s) 89, 328, 724, 1049, 1220, 2266
FspBI CTAG 1 cut(s) 119
FspI TGCGCA 1 cut(s) 2057
GlaI GCGC 5 cut(s) 280, 376, 454, 1978, 2057
GsaI CCCAGC 1 cut(s) 1476
GsuI CTGGAG 2 cut(s) 1578, 1779
HaeII RGCGCY 1 cut(s) 378
HaeIII GGCC 4 cut(s) 354, 594, 1584, 2465
HapII CCGG 7 cut(s) 355, 369, 632, 1052, 1552, 2105, 2256
HgaI GACGC 1 cut(s) 1441
HhaI GCGC 5 cut(s) 281, 377, 455, 1979, 2058
Hin6I GCGC 5 cut(s) 279, 375, 453, 1977, 2056
HinP1I GCGC 5 cut(s) 279, 375, 453, 1977, 2056
HincII GTYRAC 2 cut(s) 1194, 1944
HindII GTYRAC 2 cut(s) 1194, 1944
HindIII AAGCTT 1 cut(s) 2456
HinfI GANTC 7 cut(s) 268, 359, 702, 1133, 1544, 1794, 2471
HpaI GTTAAC 1 cut(s) 1194
HpaII CCGG 7 cut(s) 355, 369, 632, 1052, 1552, 2105, 2256
HphI GGTGA 3 cut(s) 295, 893, 1243
Hpy166II GTNNAC 5 cut(s) 124, 603, 1194, 1944, 2261
Hpy188I TCNGA 8 cut(s) 449, 478, 1076, 1111, 1412, 1799, 2338, 2371
Hpy8I GTNNAC 5 cut(s) 124, 603, 1194, 1944, 2261
Hpy99I CGWCG 4 cut(s) 238, 443, 493, 1457
HpyAV CCTTC 7 cut(s) 193, 307, 413, 473, 676, 766, 1402
HpyCH4III ACNGT 6 cut(s) 661, 881, 1339, 1351, 1933, 2507
HpyCH4IV ACGT 6 cut(s) 321, 438, 488, 605, 746, 2426
HpyF3I CTNAG 4 cut(s) 446, 1131, 1700, 1770
HpySE526I ACGT 6 cut(s) 321, 438, 488, 605, 746, 2426
HspAI GCGC 5 cut(s) 279, 375, 453, 1977, 2056
KspAI GTTAAC 1 cut(s) 1194
LguI GCTCTTC 1 cut(s) 1424
LmnI GCTCC 6 cut(s) 221, 482, 800, 1559, 2338, 2491
LweI GCATC 9 cut(s) 300, 440, 978, 1242, 1720, 1801, 1810, 2128, 2288
MabI ACCWGGT 1 cut(s) 2164
MaeI CTAG 1 cut(s) 119
MaeII ACGT 6 cut(s) 321, 438, 488, 605, 746, 2426
MaeIII GTNAC 3 cut(s) 425, 1492, 1748
MfeI CAATTG 1 cut(s) 1863
MflI RGATCY 6 cut(s) 247, 932, 1106, 1121, 2202, 2243
MhlI GDGCHC 3 cut(s) 1706, 2096, 2173
MluCI AATT 6 cut(s) 10, 364, 1299, 1405, 1863, 2063
MlyI GAGTC 3 cut(s) 353, 1142, 2480
MmeI TCCRAC 5 cut(s) 729, 1054, 1066, 1333, 2307
Mph1103I ATGCAT 1 cut(s) 1735
MseI TTAA 7 cut(s) 9, 98, 333, 1193, 2036, 2124, 2527
MslI CAYNNNNRTG 1 cut(s) 2009
MspA1I CMGCKG 1 cut(s) 274
MspI CCGG 7 cut(s) 355, 369, 632, 1052, 1552, 2105, 2256
MspR9I CCNGG 6 cut(s) 345, 356, 1052, 2079, 2166, 2299
MunI CAATTG 1 cut(s) 1863
Mva1269I GAATGC 2 cut(s) 1701, 2378
MvaI CCWGG 4 cut(s) 345, 2079, 2166, 2299
MvnI CGCG 1 cut(s) 281
NciI CCSGG 2 cut(s) 356, 1052
NcoI CCATGG 1 cut(s) 939
NdeI CATATG 1 cut(s) 1735
NmuCI GTSAC 1 cut(s) 1748
NsbI TGCGCA 1 cut(s) 2057
NsiI ATGCAT 1 cut(s) 1735
NspI RCATGY 2 cut(s) 2121, 2345
PaeI GCATGC 1 cut(s) 2345
PagI TCATGA 1 cut(s) 1344
PciI ACATGT 1 cut(s) 2117
PciSI GCTCTTC 1 cut(s) 1424
PcsI WCGNNNNNNNCGW 2 cut(s) 435, 1677
PctI GAATGC 2 cut(s) 1701, 2378
PfeI GAWTC 4 cut(s) 268, 702, 1544, 1794
PflMI CCANNNNNTGG 1 cut(s) 350
PleI GAGTC 3 cut(s) 353, 1141, 2479
PpsI GAGTC 3 cut(s) 353, 1141, 2479
Ppu21I YACGTR 1 cut(s) 606
PpuMI RGGWCCY 1 cut(s) 2349
PscI ACATGT 1 cut(s) 2117
PshAI GACNNNNGTC 1 cut(s) 1571
PsiI TTATAA 1 cut(s) 15
Psp1406I AACGTT 1 cut(s) 746
Psp5II RGGWCCY 1 cut(s) 2349
Psp6I CCWGG 4 cut(s) 343, 2077, 2164, 2297
PspFI CCCAGC 1 cut(s) 1472
PspGI CCWGG 4 cut(s) 343, 2077, 2164, 2297
PspPI GGNCC 6 cut(s) 307, 567, 1739, 2349, 2357, 2432
PspPPI RGGWCCY 1 cut(s) 2349
PstI CTGCAG 2 cut(s) 1828, 2389
PstNI CAGNNNCTG 1 cut(s) 803
PsuI RGATCY 6 cut(s) 247, 932, 1106, 1121, 2202, 2243
RsaI GTAC 9 cut(s) 320, 509, 604, 875, 1341, 1502, 1520, 1670, 2262
RsaNI GTAC 9 cut(s) 319, 508, 603, 874, 1340, 1501, 1519, 1669, 2261
RseI CAYNNNNRTG 1 cut(s) 2009
SapI GCTCTTC 1 cut(s) 1424
SaqAI TTAA 7 cut(s) 9, 98, 333, 1193, 2036, 2124, 2527
Sau96I GGNCC 6 cut(s) 307, 567, 1739, 2349, 2357, 2432
ScaI AGTACT 1 cut(s) 1502
SchI GAGTC 3 cut(s) 353, 1142, 2480
ScrFI CCNGG 6 cut(s) 345, 356, 1052, 2079, 2166, 2299
SduI GDGCHC 3 cut(s) 1706, 2096, 2173
SexAI ACCWGGT 1 cut(s) 2164
SfaNI GCATC 9 cut(s) 300, 440, 978, 1242, 1720, 1801, 1810, 2128, 2288
SfcI CTRYAG 2 cut(s) 1824, 2385
SinI GGWCC 6 cut(s) 307, 567, 1739, 2349, 2357, 2432
SmiMI CAYNNNNRTG 1 cut(s) 2009
SmlI CTYRAG 1 cut(s) 2417
SmoI CTYRAG 1 cut(s) 2417
SnaBI TACGTA 1 cut(s) 606
SphI GCATGC 1 cut(s) 2345
Sse9I AATT 6 cut(s) 10, 364, 1299, 1405, 1863, 2063
SspI AATATT 1 cut(s) 1027
SspMI CTAG 1 cut(s) 119
StyD4I CCNGG 6 cut(s) 343, 354, 1050, 2077, 2164, 2297
StyI CCWWGG 6 cut(s) 303, 562, 939, 1043, 1690, 2353
TaaI ACNGT 6 cut(s) 661, 881, 1339, 1351, 1933, 2507
TaiI ACGT 6 cut(s) 324, 441, 491, 608, 749, 2429
TaqI TCGA 4 cut(s) 408, 903, 1926, 2132
TasI AATT 6 cut(s) 10, 364, 1299, 1405, 1863, 2063
TatI WGTACW 5 cut(s) 507, 873, 1339, 1500, 2260
TauI GCSGC 2 cut(s) 375, 1844
TfiI GAWTC 4 cut(s) 268, 702, 1544, 1794
Tru1I TTAA 7 cut(s) 9, 98, 333, 1193, 2036, 2124, 2527
Tru9I TTAA 7 cut(s) 9, 98, 333, 1193, 2036, 2124, 2527
TscAI CASTG 6 cut(s) 85, 317, 696, 1753, 1843, 2101
TseFI GTSAC 1 cut(s) 1748
Tsp45I GTSAC 1 cut(s) 1748
TspDTI ATGAA 3 cut(s) 517, 728, 2027
TspGWI ACGGA 5 cut(s) 518, 559, 1594, 1977, 2478
TspRI CASTG 6 cut(s) 85, 317, 696, 1753, 1843, 2101
Van91I CCANNNNNTGG 1 cut(s) 350
VpaK11BI GGWCC 6 cut(s) 307, 567, 1739, 2349, 2357, 2432
XagI CCTNNNNNAGG 1 cut(s) 407
XceI RCATGY 2 cut(s) 2121, 2345
XcmI CCANNNNNNNNNTGG 2 cut(s) 688, 745
XspI CTAG 1 cut(s) 119
ZrmI AGTACT 1 cut(s) 1502
Zsp2I ATGCAT 1 cut(s) 1735
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.