MD02G1020700.v1.1

Pyrophosphate-energized vacuolar membrane proton

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0000897
Physical Location & Seq
Forward (+)
1469475 .. 1474025
4551 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1020700.v1.1.491

Sequence Viewer

Length: 2295 bp
ATGGGGGCGACGATACTCCCAGATCTCTGGGCCGAGATTTTGATCCCGCTGTGTGCGGTGATCGGCATCGCCTTCTCCTTGGTCCAGTGGATGTACGTTTCGCAGGTCAAGCTCTCCCCCGGCGGCGGCCGGGACTCCAACTCCGGGAAAAATGGGCACAACGAGTACCTCATCGAGGAAGAAGAAGGCGGTAACGACCACAACGTCGTCCTCAAATGCGCCGAAATCCAAAACGCCATTTCAGAAGGAGCAACCTCGTTCCTCTTCACGGAATACAGATATGTAGGTGCTTTCATGGTTGCTTTTGCAATCTTGATCTTCCTTTTCCTTGGATCCGTCGAGGGATTTAGTACAAGTAGCCGTGCATGTACATATGACCAAACCAAGACGTGCAAACCAGCTCTCGCCACTGCCATCTTCAGCACCATTTCCTTTTTGCTTGGTGCTGTAACTTCAGTGGTTTCTGGATTCCTTGGGATGAAGATTGCTACTTACGCAAATGCCAGAACAACGTTGGAGGCAAGAAAAGGTGTTGGGAAGGCTTTTATTACTGCATTTAGATCAGGAGCTGTGATGGGCTTTCTCCTCGCTTCAAGTGGTCTGTTGGTTCTTTACATTGCTATCAATGTCTTCAAGTTGTACTACGGTGATGACTGGGGTGGTCTTTTCGAGGCTATAACTGGTTATGGTCTTGGTGGATCTTCCATGGCTCTCTTTGGCAGGGTTGGTGGAGGCATCTATACCAAAGCTGCTGATGTTGGTGCAGATCTTGTCGGCAAGGTTGAAAGAAATATTCCCGAGGATGACCCAAGGAACCCAGCTGTGATAGCTGATAATGTTGGTGACAATGTTGGAGATATAGCTGGCATGGGATCTGATCTTTTTGGATCCTATGCTGAGTCATCGTGTGCTGCCCTTGTGGTTGCTTCCATTTCCTCATTTGGGATCAATCACGAGCTAACTGCAATGCTATATCCTCTCATTGTCAGTTCCATCGGCATCATTGTTTGTTTGCTCACTACCCTATTTGCAACCGATTTCTTTGAGATCAAGGCTGTAAAGGAAATTGAGCCAACATTGAAGAGGCAGCTCATAATCTCCACTGTACTCATGACCGTTGGAGTTGCGATTGCTACATGGATTTCCGTTCCATCTTCCTTCACAATCTTCAATTTCGGAACACAGAAAGTTGTGAAGAACTGGCAGCTATTCTTATGTGTCTGTGTTGGTTTGTGGGCTGGCCTTATCATTGGGTTTGTAACAGAGTATTATACAAGCAATGCGTACAGCCCTGTGCAAGATGTTGCTGATTCTTGCCGGACTGGAGCTGCGACCAATGTCATTTTTGGCCTTGCATTGGGATACAAATCCGTCATTATTCCTATTTTTGCCATTGCAGTCAGCATTTTTGTTAGTTTTAGCTTTGCAGCTATGTACGGGATTGCGGTAGCTGCCCTTGGAATGCTGAGCACCATTGCAACTGGATTAGCGATTGATGCATATGGTCCCATCAGTGACAATGCTGGAGGCATTGCTGAGATGGCAGGCATGAGCCACAGAATAAGAGAGAGAACTGATGCTCTTGATGCTGCAGGAAACACCACTGCTGCTATTGGAAAGGGATTTGCAATTGGCTCTGCTGCTCTTGTGTCCCTTGCTCTATTTGGTGCCTTTGTCAGCCGTGCTGGTATCAAAACAGTTGATGTGTTGACCCCGAAAGTGATTATTGGTTTGTTAGTGGGCGCAATGCTTCCGTATTGGTTCTCTGCCATGACAATGAAGAGTGTGGGAAGTGCAGCTCTAAAGATGGTTGAGGAAGTGCGCCGGCAATTCAATACCATTCCCGGTCTCATGGAGGGCACTGCCAAGCCGGACTACGCTACATGTGTTAAGATTTCGACTGATGCTTCCATCAAGGAAATGATCCCGCCTGGTGCCCTTGTCATGCTTACACCGCTCATTGTTGGGATCTTTTTTGGTGTGGAAACTCTCGCTGGCGTTCTTGCTGGATCCCTTGTGTCCGGTGTACAGATTGCCATTTCTGCGTCCAACACGGGCGGTGCCTGGGATAATGCCAAGAAATACATTGAGGCTGGTGCGTCGGAGCATGCAAGGACCCTTGGTCCAAAAGGATCAGAATGCCACAAAGCTGCTGTCATCGGTGACACCATTGGAGACCCTCTCAAGGATACATCTGGACCATCGCTCAACATTCTCATCAAGCTCATGGCCGTTGAGTCTCTTGTGTTTGCTCCGTTCTTTGCCACACACGGTGGCCTGCTTTTCAAGATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 map00190
Pfam Domains
Protein Families
H_PPase

Protein Analysis

765

Amino Acids

80.04

Weight (kDa)

5.47

Isoelectric Point (pI)

25.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
H_PPase PF03030 64 - 750 3.3e-256 Inorganic H+ pyrophosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 203
Acc36I ACCTGC 1 cut(s) 94
AccB1I GGYRCC 3 cut(s) 1667, 1934, 2060
AccBSI CCGCTC 1 cut(s) 1957
AciI CCGC 9 cut(s) 47, 56, 123, 126, 189, 1445, 1928, 1955, 2058
AclI AACGTT 1 cut(s) 512
AcoI YGGCCR 2 cut(s) 127, 2229
AcuI CTGAAG 2 cut(s) 403, 438
AdeI CACNNNGTG 1 cut(s) 2273
AfaI GTAC 9 cut(s) 95, 167, 352, 370, 641, 1107, 1286, 1436, 2028
AfiI CCNNNNNNNGG 7 cut(s) 125, 144, 175, 268, 942, 1357, 2185
AflIII ACRYGT 1 cut(s) 1883
AgsI TTSAA 7 cut(s) 594, 634, 785, 1081, 1171, 1834, 2287
AhdI GACNNNNNGTC 1 cut(s) 2121
AjiI CACGTC 1 cut(s) 390
AjnI CCWGG 2 cut(s) 1930, 2063
Alw21I GWGCWC 1 cut(s) 1472
Alw26I GTCTC 3 cut(s) 1853, 2169, 2244
AlwNI CAGNNNCTG 1 cut(s) 569
Ama87I CYCGRG 1 cut(s) 797
AoxI GGCC 6 cut(s) 30, 127, 1240, 1348, 2229, 2275
ArsI GACNNNNNNTTYG 2 cut(s) 2139, 2171
AspLEI GCGC 3 cut(s) 221, 1745, 1824
AspS9I GGNCC 6 cut(s) 30, 82, 1505, 2115, 2123, 2198
AsuC2I CCSGG 4 cut(s) 120, 131, 145, 1845
AsuHPI GGTGA 4 cut(s) 70, 659, 854, 2174
AvaI CYCGRG 1 cut(s) 797
AvaII GGWCC 5 cut(s) 82, 1505, 2115, 2123, 2198
BaeGI GKGCMC 3 cut(s) 159, 1862, 1939
BamHI GGATCC 3 cut(s) 332, 887, 2009
BanI GGYRCC 3 cut(s) 1667, 1934, 2060
BauI CACGAG 1 cut(s) 953
BbsI GAAGAC 1 cut(s) 622
Bbv12I GWGCWC 1 cut(s) 1472
BccI CCATC 9 cut(s) 422, 568, 1001, 1159, 1517, 1534, 1801, 1919, 2209
BceAI ACGGC 3 cut(s) 345, 1665, 2216
BcgI CGANNNNNNTGC 2 cut(s) 944, 978
BciT130I CCWGG 2 cut(s) 1932, 2065
BciVI GTATCC 2 cut(s) 1355, 2182
BcnI CCSGG 4 cut(s) 120, 131, 145, 1845
BcoDI GTCTC 3 cut(s) 1853, 2169, 2244
BfmI CTRYAG 1 cut(s) 1590
BfuAI ACCTGC 1 cut(s) 94
BfuI GTATCC 2 cut(s) 1355, 2182
BglII AGATCT 2 cut(s) 22, 766
BlpI GCTNAGC 1 cut(s) 1466
Bme1390I CCNGG 6 cut(s) 120, 131, 145, 1845, 1932, 2065
Bme18I GGWCC 5 cut(s) 82, 1505, 2115, 2123, 2198
BmeRI GACNNNNNGTC 1 cut(s) 2121
BmeT110I CYCGRG 1 cut(s) 797
BmgBI CACGTC 1 cut(s) 390
BmgT120I GGNCC 6 cut(s) 30, 82, 1505, 2115, 2123, 2198
BmiI GGNNCC 9 cut(s) 334, 815, 889, 1507, 1669, 1936, 2011, 2062, 2117
BmrFI CCNGG 6 cut(s) 120, 131, 145, 1845, 1932, 2065
BmrI ACTGGG 1 cut(s) 664
BmsI GCATC 7 cut(s) 75, 744, 1008, 1486, 1567, 1576, 1894
BmuI ACTGGG 1 cut(s) 664
BoxI GACNNNNGTC 1 cut(s) 1337
BpiI GAAGAC 1 cut(s) 622
BplI GAGNNNNNCTC 2 cut(s) 2166, 2198
BpmI CTGGAG 2 cut(s) 1344, 1545
Bpu1102I GCTNAGC 1 cut(s) 1466
BpuEI CTTGAG 1 cut(s) 2168
BpuMI CCSGG 4 cut(s) 120, 131, 145, 1845
BsaBI GATNNNNATC 3 cut(s) 41, 65, 1366
BsaI GGTCTC 2 cut(s) 1853, 2169
BsaWI WCCGGW 1 cut(s) 2021
BsaXI ACNNNNNCTCC 2 cut(s) 125, 155
Bsc4I CCNNNNNNNGG 7 cut(s) 125, 144, 175, 268, 942, 1357, 2185
Bse118I RCCGGY 1 cut(s) 1824
Bse1I ACTGG 6 cut(s) 85, 659, 685, 1205, 1327, 1486
Bse3DI GCAATG 7 cut(s) 615, 972, 1285, 1392, 1473, 1530, 1752
Bse8I GATNNNNATC 3 cut(s) 41, 65, 1366
BseBI CCWGG 2 cut(s) 1932, 2065
BseGI GGATG 3 cut(s) 96, 483, 808
BseJI GATNNNNATC 3 cut(s) 41, 65, 1366
BseLI CCNNNNNNNGG 7 cut(s) 125, 144, 175, 268, 942, 1357, 2185
BseMI GCAATG 7 cut(s) 615, 972, 1285, 1392, 1473, 1530, 1752
BseMII CTCAG 3 cut(s) 888, 1457, 1527
BseNI ACTGG 6 cut(s) 85, 659, 685, 1205, 1327, 1486
BseRI GAGGAG 1 cut(s) 575
BseSI GKGCMC 3 cut(s) 159, 1862, 1939
BseX3I CGGCCG 1 cut(s) 127
BseYI CCCAGC 1 cut(s) 817
BsgI GTGCAG 2 cut(s) 783, 1815
Bsh1285I CGRYCG 1 cut(s) 130
BshFI GGCC 6 cut(s) 32, 129, 1242, 1350, 2231, 2277
BshNI GGYRCC 3 cut(s) 1667, 1934, 2060
BsiEI CGRYCG 1 cut(s) 130
BsiHKAI GWGCWC 1 cut(s) 1472
BsiHKCI CYCGRG 1 cut(s) 797
BsiSI CCGG 8 cut(s) 120, 130, 144, 1318, 1825, 1845, 1871, 2022
BslFI GGGAC 3 cut(s) 146, 1491, 1636
BslI CCNNNNNNNGG 7 cut(s) 125, 144, 175, 268, 942, 1357, 2185
BsmAI GTCTC 3 cut(s) 1853, 2169, 2244
BsmFI GGGAC 3 cut(s) 146, 1491, 1636
BsmI GAATGC 2 cut(s) 1467, 2144
BsnI GGCC 6 cut(s) 32, 129, 1242, 1350, 2231, 2277
Bso31I GGTCTC 2 cut(s) 1853, 2169
BsoBI CYCGRG 1 cut(s) 797
Bsp1286I GDGCHC 4 cut(s) 159, 1472, 1862, 1939
Bsp1407I TGTACA 2 cut(s) 368, 2026
Bsp1720I GCTNAGC 1 cut(s) 1466
Bsp19I CCATGG 1 cut(s) 705
BspACI CCGC 9 cut(s) 47, 56, 123, 126, 189, 1445, 1928, 1955, 2058
BspANI GGCC 6 cut(s) 32, 129, 1242, 1350, 2231, 2277
BspCNI CTCAG 3 cut(s) 889, 1458, 1528
BspHI TCATGA 1 cut(s) 1110
BspLI GGNNCC 9 cut(s) 334, 815, 889, 1507, 1669, 1936, 2011, 2062, 2117
BspMAI CTGCAG 1 cut(s) 1594
BspMI ACCTGC 1 cut(s) 94
BspT107I GGYRCC 3 cut(s) 1667, 1934, 2060
BspTNI GGTCTC 2 cut(s) 1853, 2169
BsrBI CCGCTC 1 cut(s) 1957
BsrDI GCAATG 7 cut(s) 615, 972, 1285, 1392, 1473, 1530, 1752
BsrFI RCCGGY 1 cut(s) 1824
BsrGI TGTACA 2 cut(s) 368, 2026
BsrI ACTGG 6 cut(s) 85, 659, 685, 1205, 1327, 1486
BssAI RCCGGY 1 cut(s) 1824
BssSI CACGAG 1 cut(s) 953
BssT1I CCWWGG 7 cut(s) 78, 328, 472, 705, 809, 1456, 2119
Bst2BI CACGAG 1 cut(s) 953
Bst2UI CCWGG 2 cut(s) 1932, 2065
Bst4CI ACNGT 5 cut(s) 647, 1105, 1117, 1699, 2273
Bst6I CTCTTC 3 cut(s) 269, 1076, 1775
BstAUI TGTACA 2 cut(s) 368, 2026
BstC8I GCNNGC 7 cut(s) 865, 1240, 1546, 1826, 1996, 2109, 2279
BstDEI CTNAG 3 cut(s) 897, 1466, 1536
BstDSI CCRYGG 1 cut(s) 705
BstENI CCTNNNNNAGG 1 cut(s) 173
BstF5I GGATG 3 cut(s) 96, 483, 808
BstHHI GCGC 3 cut(s) 221, 1745, 1824
BstMAI GTCTC 3 cut(s) 1853, 2169, 2244
BstMCI CGRYCG 1 cut(s) 130
BstMWI GCNNNNNNNGC 9 cut(s) 109, 494, 827, 1451, 1496, 1541, 1586, 1954, 2042
BstNI CCWGG 2 cut(s) 1932, 2065
BstNSI RCATGY 3 cut(s) 369, 1887, 2111
BstPAI GACNNNNGTC 1 cut(s) 1337
BstSCI CCNGG 6 cut(s) 118, 129, 143, 1843, 1930, 2063
BstSFI CTRYAG 1 cut(s) 1590
BstSLI GKGCMC 3 cut(s) 159, 1862, 1939
BstV2I GAAGAC 1 cut(s) 622
BstX2I RGATCY 8 cut(s) 22, 332, 698, 766, 872, 887, 1968, 2009
BstXI CCANNNNNNTGG 1 cut(s) 27
BstYI RGATCY 8 cut(s) 22, 332, 698, 766, 872, 887, 1968, 2009
BstZI CGGCCG 1 cut(s) 127
BsuI GTATCC 2 cut(s) 1355, 2182
BsuRI GGCC 6 cut(s) 32, 129, 1242, 1350, 2231, 2277
BtgI CCRYGG 1 cut(s) 705
BtgZI GCGATG 2 cut(s) 52, 2187
BtrI CACGTC 1 cut(s) 390
BtsCI GGATG 3 cut(s) 96, 483, 808
BtsI GCAGTG 3 cut(s) 408, 1602, 1860
BtsIMutI CAGTG 7 cut(s) 92, 408, 462, 1101, 1519, 1602, 1860
BveI ACCTGC 1 cut(s) 94
Cac8I GCNNGC 7 cut(s) 865, 1240, 1546, 1826, 1996, 2109, 2279
CaiI CAGNNNCTG 1 cut(s) 569
CciI TCATGA 1 cut(s) 1110
CfoI GCGC 3 cut(s) 221, 1745, 1824
Cfr10I RCCGGY 1 cut(s) 1824
Cfr13I GGNCC 6 cut(s) 30, 82, 1505, 2115, 2123, 2198
CseI GACGC 2 cut(s) 2034, 2088
Csp6I GTAC 9 cut(s) 94, 166, 351, 369, 640, 1106, 1285, 1435, 2027
CviQI GTAC 9 cut(s) 94, 166, 351, 369, 640, 1106, 1285, 1435, 2027
DdeI CTNAG 3 cut(s) 897, 1466, 1536
DraIII CACNNNGTG 1 cut(s) 2273
DrdI GACNNNNNNGTC 1 cut(s) 203
DriI GACNNNNNGTC 1 cut(s) 2121
DseDI GACNNNNNNGTC 1 cut(s) 203
EaeI YGGCCR 2 cut(s) 127, 2229
EagI CGGCCG 1 cut(s) 127
Eam1104I CTCTTC 3 cut(s) 269, 1076, 1775
Eam1105I GACNNNNNGTC 1 cut(s) 2121
EarI CTCTTC 3 cut(s) 269, 1076, 1775
EclXI CGGCCG 1 cut(s) 127
Eco130I CCWWGG 7 cut(s) 78, 328, 472, 705, 809, 1456, 2119
Eco31I GGTCTC 2 cut(s) 1853, 2169
Eco47I GGWCC 5 cut(s) 82, 1505, 2115, 2123, 2198
Eco52I CGGCCG 1 cut(s) 127
Eco57I CTGAAG 2 cut(s) 403, 438
Eco88I CYCGRG 1 cut(s) 797
EcoNI CCTNNNNNAGG 1 cut(s) 173
EcoO109I RGGNCCY 1 cut(s) 2115
EcoRII CCWGG 2 cut(s) 1930, 2063
EcoT14I CCWWGG 7 cut(s) 78, 328, 472, 705, 809, 1456, 2119
EcoT22I ATGCAT 1 cut(s) 1501
ErhI CCWWGG 7 cut(s) 78, 328, 472, 705, 809, 1456, 2119
FaqI GGGAC 3 cut(s) 146, 1491, 1636
FauI CCCGC 2 cut(s) 54, 1935
FauNDI CATATG 2 cut(s) 373, 1501
FokI GGATG 3 cut(s) 103, 490, 815
GlaI GCGC 3 cut(s) 220, 1744, 1823
GsaI CCCAGC 1 cut(s) 821
GsuI CTGGAG 2 cut(s) 1344, 1545
HaeIII GGCC 6 cut(s) 32, 129, 1242, 1350, 2231, 2277
HapII CCGG 8 cut(s) 120, 130, 144, 1318, 1825, 1845, 1871, 2022
HgaI GACGC 2 cut(s) 2034, 2088
HhaI GCGC 3 cut(s) 221, 1745, 1824
Hin6I GCGC 3 cut(s) 219, 1743, 1822
HinP1I GCGC 3 cut(s) 219, 1743, 1822
HincII GTYRAC 1 cut(s) 1710
HindII GTYRAC 1 cut(s) 1710
HinfI GANTC 5 cut(s) 134, 468, 899, 1310, 2237
HpaII CCGG 8 cut(s) 120, 130, 144, 1318, 1825, 1845, 1871, 2022
HphI GGTGA 4 cut(s) 70, 659, 854, 2174
Hpy166II GTNNAC 2 cut(s) 1710, 2027
Hpy188I TCNGA 5 cut(s) 244, 877, 1178, 2104, 2137
Hpy188III TCNNGA 9 cut(s) 313, 465, 564, 797, 953, 1111, 1583, 2196, 2287
Hpy8I GTNNAC 2 cut(s) 1710, 2027
Hpy99I CGWCG 4 cut(s) 13, 209, 341, 2104
HpyAV CCTTC 5 cut(s) 82, 179, 239, 532, 1168
HpyCH4III ACNGT 5 cut(s) 647, 1105, 1117, 1699, 2273
HpyCH4IV ACGT 4 cut(s) 96, 204, 389, 512
HpyF10VI GCNNNNNNNGC 9 cut(s) 109, 494, 827, 1451, 1496, 1541, 1586, 1954, 2042
HpyF3I CTNAG 3 cut(s) 897, 1466, 1536
HpySE526I ACGT 4 cut(s) 96, 204, 389, 512
HspAI GCGC 3 cut(s) 219, 1743, 1822
KroI GCCGGC 1 cut(s) 1824
KroNI GCCGGC 1 cut(s) 1826
LmnI GCTCC 5 cut(s) 248, 566, 1325, 2104, 2257
LweI GCATC 7 cut(s) 75, 744, 1008, 1486, 1567, 1576, 1894
MaeII ACGT 4 cut(s) 96, 204, 389, 512
MaeIII GTNAC 6 cut(s) 191, 448, 842, 1258, 1514, 2162
MbiI CCGCTC 1 cut(s) 1957
MfeI CAATTG 1 cut(s) 1629
MflI RGATCY 8 cut(s) 22, 332, 698, 766, 872, 887, 1968, 2009
MhlI GDGCHC 4 cut(s) 159, 1472, 1862, 1939
MluCI AATT 4 cut(s) 1065, 1171, 1629, 1829
MlyI GAGTC 3 cut(s) 128, 908, 2246
MmeI TCCRAC 6 cut(s) 162, 495, 832, 1099, 2073, 2082
Mph1103I ATGCAT 1 cut(s) 1501
MroNI GCCGGC 1 cut(s) 1824
MseI TTAA 2 cut(s) 1890, 2293
MslI CAYNNNNRTG 1 cut(s) 1775
MspA1I CMGCKG 2 cut(s) 49, 821
MspI CCGG 8 cut(s) 120, 130, 144, 1318, 1825, 1845, 1871, 2022
MspR9I CCNGG 6 cut(s) 120, 131, 145, 1845, 1932, 2065
MunI CAATTG 1 cut(s) 1629
Mva1269I GAATGC 2 cut(s) 1467, 2144
MvaI CCWGG 2 cut(s) 1932, 2065
MwoI GCNNNNNNNGC 9 cut(s) 109, 494, 827, 1451, 1496, 1541, 1586, 1954, 2042
NaeI GCCGGC 1 cut(s) 1826
NciI CCSGG 4 cut(s) 120, 131, 145, 1845
NcoI CCATGG 1 cut(s) 705
NdeI CATATG 2 cut(s) 373, 1501
NgoMIV GCCGGC 1 cut(s) 1824
NlaIV GGNNCC 9 cut(s) 334, 815, 889, 1507, 1669, 1936, 2011, 2062, 2117
NmeAIII GCCGAG 1 cut(s) 58
NmuCI GTSAC 3 cut(s) 842, 1514, 2162
NsiI ATGCAT 1 cut(s) 1501
NspI RCATGY 3 cut(s) 369, 1887, 2111
PaeI GCATGC 1 cut(s) 2111
PagI TCATGA 1 cut(s) 1110
PciI ACATGT 1 cut(s) 1883
PcsI WCGNNNNNNNCGW 1 cut(s) 201
PctI GAATGC 2 cut(s) 1467, 2144
PdiI GCCGGC 1 cut(s) 1826
PfeI GAWTC 2 cut(s) 468, 1310
PfoI TCCNGGA 1 cut(s) 143
PleI GAGTC 3 cut(s) 128, 907, 2245
PpsI GAGTC 3 cut(s) 128, 907, 2245
PpuMI RGGWCCY 1 cut(s) 2115
PscI ACATGT 1 cut(s) 1883
PshAI GACNNNNGTC 1 cut(s) 1337
Psp1406I AACGTT 1 cut(s) 512
Psp5II RGGWCCY 1 cut(s) 2115
Psp6I CCWGG 2 cut(s) 1930, 2063
PspFI CCCAGC 1 cut(s) 817
PspGI CCWGG 2 cut(s) 1930, 2063
PspN4I GGNNCC 9 cut(s) 334, 815, 889, 1507, 1669, 1936, 2011, 2062, 2117
PspPI GGNCC 6 cut(s) 30, 82, 1505, 2115, 2123, 2198
PspPPI RGGWCCY 1 cut(s) 2115
PstI CTGCAG 1 cut(s) 1594
PstNI CAGNNNCTG 1 cut(s) 569
PsuI RGATCY 8 cut(s) 22, 332, 698, 766, 872, 887, 1968, 2009
PvuII CAGCTG 1 cut(s) 821
RsaI GTAC 9 cut(s) 95, 167, 352, 370, 641, 1107, 1286, 1436, 2028
RsaNI GTAC 9 cut(s) 94, 166, 351, 369, 640, 1106, 1285, 1435, 2027
RseI CAYNNNNRTG 1 cut(s) 1775
SaqAI TTAA 2 cut(s) 1890, 2293
Sau96I GGNCC 6 cut(s) 30, 82, 1505, 2115, 2123, 2198
SchI GAGTC 3 cut(s) 128, 908, 2246
ScrFI CCNGG 6 cut(s) 120, 131, 145, 1845, 1932, 2065
SduI GDGCHC 4 cut(s) 159, 1472, 1862, 1939
SfaNI GCATC 7 cut(s) 75, 744, 1008, 1486, 1567, 1576, 1894
SfcI CTRYAG 1 cut(s) 1590
SinI GGWCC 5 cut(s) 82, 1505, 2115, 2123, 2198
SmiMI CAYNNNNRTG 1 cut(s) 1775
SmlI CTYRAG 1 cut(s) 2183
SmoI CTYRAG 1 cut(s) 2183
SphI GCATGC 1 cut(s) 2111
Sse9I AATT 4 cut(s) 1065, 1171, 1629, 1829
SsiI CCGC 9 cut(s) 47, 56, 123, 126, 189, 1445, 1928, 1955, 2058
SspI AATATT 1 cut(s) 793
StyD4I CCNGG 6 cut(s) 118, 129, 143, 1843, 1930, 2063
StyI CCWWGG 7 cut(s) 78, 328, 472, 705, 809, 1456, 2119
TaaI ACNGT 5 cut(s) 647, 1105, 1117, 1699, 2273
TaiI ACGT 4 cut(s) 99, 207, 392, 515
TaqI TCGA 4 cut(s) 174, 339, 669, 1898
TasI AATT 4 cut(s) 1065, 1171, 1629, 1829
TatI WGTACW 5 cut(s) 350, 368, 639, 1105, 2026
TauI GCSGC 2 cut(s) 126, 129
TfiI GAWTC 2 cut(s) 468, 1310
Tru1I TTAA 2 cut(s) 1890, 2293
Tru9I TTAA 2 cut(s) 1890, 2293
TscAI CASTG 7 cut(s) 92, 415, 462, 1108, 1519, 1609, 1867
TseFI GTSAC 3 cut(s) 842, 1514, 2162
Tsp45I GTSAC 3 cut(s) 842, 1514, 2162
TspDTI ATGAA 3 cut(s) 283, 494, 1793
TspGWI ACGGA 6 cut(s) 284, 325, 1135, 1360, 1743, 2244
TspRI CASTG 7 cut(s) 92, 415, 462, 1108, 1519, 1609, 1867
VpaK11BI GGWCC 5 cut(s) 82, 1505, 2115, 2123, 2198
XagI CCTNNNNNAGG 1 cut(s) 173
XceI RCATGY 3 cut(s) 369, 1887, 2111
XcmI CCANNNNNNNNNTGG 1 cut(s) 511
Zsp2I ATGCAT 1 cut(s) 1501
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.