FvH4_1g06870

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0002206
Physical Location & Seq
Reverse (-)
3630577 .. 3638209
7633 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g06870.t1

Sequence Viewer

Length: 3606 bp
ATGGGAGCTTCTCCGGATGCCAACGGAGATATAGGTATGAGTAGCAAGGGAAAAGGCTTTTACAATGGGAATTTTGATGACATGGATATTGATGACCTGGATTTTGGTGATGAAGAAGTTGAGGATTCGACTACCTTACATGATCTGGGAGAAGTGTTCCTGGACGAATTCTGTAAGGAGGCGTCAGTGTCATTCTTCAAGGAGTATGGCCTCCTAAGTCATCAGATTAATTCATACAATGACTTTGTTGAGAATGGCATACAAAGGGTCTTTGATTCTTTCGGTGAGATTGTAGTTGAGCCTAATTTTGATCCATCAAAGAAGGGAAATGCTGACTGGCGATTTGCAGCTATCAAGTTTGGAAAAGTCACCCTTAGTAAGCCATGTTTTTGGGGAGGTTCAGATAGTGACAAGGAGTATAACATGTTACCAAGGCACGCTAGGCTTCAGAACATGACGTACTCAGCCAGGATGTCTGTCAATATTACTGTCCAGGTATATACTCAAAATCTTGTCGAAAGTGACAAGTTTAAAACTGGAACAGAGCAACTACTGGAAAAGACGACTCTGAGTACTGACAGCAGGGACATCATAATTGGTAGGATTCCTGTAATGGTGAAGTCTAACTTGTGTTGGATGAAGGGAGTCGAGAAAGGGGACTGTGAGTTTGACCAAGGAGGCTACTTTATAATAAATGGTGCAGAGAAGACATTCATAGCTCAAGAACAGAGCTGCTTGAAGAGGCTTCTGATTACAAATAGTCAGGGTCTCACAGTAGCATATAGGTCAGAGGTCAAGCGACATAGGCTCATAATTAGACTATCCGGGATTTCGAAGCTTGAAAATGTTGAAGGAGTGGAGAAAGTCCTGTCAGTATATTTTATGTCAACAGAAATTCCTGTTTGGATTTGGTTTTTTGCCCTTGGGGTTAGTTCAGACAAAGAAGTTATTGATCTGATTGATTATGGCATTGAAGATGCAAGCATTTCAAACATACTGTTTGCCTCAATCCGTGATGCAGATGAGCGAAAAGATGACTTTCGTAGGGGGAATAATGCTCTGAGATATGTTGAAAATGCAATAAAGAGTACGACATTTCCACCTGGGGAAAGCGTGGAAGACTGCATCAGCATGTATCTGTTTCCTAATATGAGAGGCTTGAAGCAGAAGGCTCGCTTTCTAGGGTATATGGTGAAGTGTCTTTTACAGACCTCCATTGGCCTAAGAAAATGCGATAATAGGGATGACTTTAGAAACAAGAGATTGGAGTTAGCAGGTGAGCTTCTAGAACGAGAGCTGAAGGCACACGTTTCACATGCACGGAGGAAAATGGCAAAGGCTCTGCAAAGAGACCTAGGCGGTGATCATGTTGTCCGGCCAGTGGAACACTATCTAGATGCTTCCATAGTTACCAATGGTGTTTCCCGAGCATTCTCTACTGGAGCATGGTGTCATCCTTTCAAGAGGATGGAGAGGATATCAGGTGTGGTGGCAAATATTGGGCGAGCAAATCCCTTGCAGACAATGATTGATATGAGGAAGACACGCCAGCAAGTTCAATACACTGGCAAGGCTGGAGATGCCAGATATCCGCATCCTTCTCACTGGGGGAAGGTTTGCTTTCTTTCAACCCCGGATGGTGAGAACTGTGGACTTGTGAAAAATTTGGCTACCACTGCGCTTGTAAGTATAAACATATCGGAGCCCATATTGCCCAAATTGTTCAAATGCGGCATAGAGGAGTTGGTAGATGATACCTATACTTCACTGGCTGGGAAGCATAAAGTTTTTCTGAATGGTGACTGGGTTGGCATTTGTGTAGACTCACATGGTTTTGTAACAAAACTAAGGAGGTTGCGACGCAGTAAAAAGTTACCACATCAGGTGGAAATCAAAAGAGACGAACACAAAGGAGAAGTGCGCATATTTTCTGATGCTGGAAGGATTCTACGTCCGCTCTTGGTTGTTCAGGGTTTGAAAAAGATTAAAGCAGCAAAAGGAGAAAAGCATACCTTCCAAGATTTTCTCGACAAGGGAATAATTGAGTTCATTGGTGCAGAAGAAGAAGAGGACTGCCGTACAGCATGGGCCATTAAATATCTATTTATGGAAGAACAGGGCTCGGTGGTCAAGTACACACACTGTGAACTGGACATGTCCTTTTTGTTAGGTTTGAGCTGTGGTATAATTCCCTTTGCAAATCATGATCATGCAAGGAGGGTGCTCTACCAGTCTCAGAAGCACTCTCAGCAGGCTATTGGTTATTCTACTACAAATCCGAACCTCAGAGTGGATACGTTGTCCCACAACTTGCACTACCCCCAACGGCCGCTTTTTCGTACAATGATAGCTGATTCCCTTGGAAAACCAGGATACCCAGTGGGACATAACGGTTTTATGCCAAAGCCTGAGTACTTCAATGGTCAGAATGCAATTGTTGCAGTCAATGTGCACCTTGGTTATAACCAAGAAGATTCTATAGTAATGAACCGGGCATCACTGGAACGTGGTATGTTTCGTTCTGAGCACATCAGGAGTTACAAGGCTGAGGTTGAAAACAAGCTCTCATTGGAAAAAAGACGAAAACCAGATGAGTGTGTCAACTTTGGCAAAATCCAGAGTAAATTTGGACGTGTTGACAACCTTGATGACGATGGGTTTCCTTGTGTTGGTGCAAGTCTCCAGAGTGGAGATATCATTATCGGGAGGTGTAGTGAATCTGGTACCGATCATAGCATCAAATTAAAGCATACTGAAAGGGGCATGGTTCAGAAGGTTGTGCTTTCCTCTAATGATGCTGGCAAGAACTTTGCGGTTGTATCATTAAGACAGGTACGTTCTCCTTCTCTTGGAGACAAATTCTCTAGCATGCACGGGCAGAAGGGGGTCCTCGGTTTTTTGGAGTATCAGGAGAACTTCCCTTTCACAATACAAGGGATAGTTCCAGATATTGTAATTAATCCACATGCATTCCCTTCAAGACAAACTCCGGGTCAACTCCTAGAGGCTGCTTTAGGGAAGGGGATTGCCTGTGGTGGTTCAAAGAGATATGCCACACCCTTCTCCACTCTCTCTGTTGATGACATCACAGACCAGCTTCATAGAGCTGGATTTTCAAGATGGGGAAATGAGAGAGTGTACAATGGCAGAACTGGTGAAATGGTTCGCCAGCTGATTTTTATGGGCCCAACATTCTATCAACGGCTGATTCACATGTCTGAAGATAAAGTTAAGTTTAGGAACACTGGGCCAGTCCACCCTCTCACCCGGCAGCCAGTGGCAGACAGGAAGCGATTTGGTGGGATCAAGTTTGGTGAGATGGAGCGCGACTGCCTGATAGCCCATGGAGCATCAGCAAACTTGCATGAACGCCTCTTTACTCTCAGTGACTCCTCCCTGATGAACGTTTGCCAGAAATGCCAGAATGCTGCTAATGTGATAGAGCGGAAATTGACTGGCGGCCGCAAAATCAGGGGTCCATATTGCCGGGTCTGCGACTCTGCTGATGACATTGTGAGGGTGAATGTGCCATATGGGGCCAAGCTATTGTGCCAGGAGCTATTCAGCATGGGGATAAGTCTCAAGTTTGAAACCAGGCTTTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000228 GO:0000418 GO:0000419 GO:0000428 GO:0000785 GO:0000790 GO:0000792 GO:0002682 GO:0003674 GO:0003824 GO:0003899 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005720 GO:0005730 GO:0005736 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009605 GO:0009607 GO:0009620 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016020 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019222 GO:0019438 GO:0030422 GO:0030880 GO:0031047 GO:0031050 GO:0031974 GO:0031981 GO:0032259 GO:0032774 GO:0032991 GO:0034062 GO:0034641 GO:0034654 GO:0035194 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043331 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044451 GO:0044452 GO:0044454 GO:0044464 GO:0044728 GO:0046483 GO:0048519 GO:0048583 GO:0050776 GO:0050789 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0055029 GO:0060255 GO:0061695 GO:0065007 GO:0070013 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0097747 GO:0098542 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1901698 GO:1901699 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko01100 ko03020 ko05016 ko05169 map00230 map00240 map01100 map03020 map05016 map05169
Pfam Domains
Protein Families
RNA_pol_Rpb2_1 RNA_pol_Rpb2_2 RNA_pol_Rpb2_3 RNA_pol_Rpb2_4 RNA_pol_Rpb2_5 RNA_pol_Rpb2_6 RNA_pol_Rpb2_7

Protein Analysis

1202

Amino Acids

135.13

Weight (kDa)

8.56

Isoelectric Point (pI)

36.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNA_pol_Rpb2_1 PF04563 70 - 456 5.1e-37 RNA polymerase beta subunit
RNA_pol_Rpb2_2 PF04561 284 - 423 1.5e-11 RNA polymerase Rpb2, domain 2
RNA_pol_Rpb2_3 PF04565 502 - 561 6.4e-15 RNA polymerase Rpb2, domain 3
RNA_pol_Rpb2_4 PF04566 596 - 657 4.6e-23 RNA polymerase Rpb2, domain 4
RNA_pol_Rpb2_5 PF04567 672 - 718 2.9e-07 RNA polymerase Rpb2, domain 5
RNA_pol_Rpb2_6 PF00562 727 - 1098 2.5e-110 RNA polymerase Rpb2, domain 6
RNA_pol_Rpb2_7 PF04560 1100 - 1197 2.8e-26 RNA polymerase Rpb2, domain 7
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 689, 2463
AarI CACCTGC 1 cut(s) 1265
Acc16I TGCGCA 1 cut(s) 1922
Acc36I ACCTGC 1 cut(s) 1265
Acc65I GGTACC 1 cut(s) 2723
AccB1I GGYRCC 1 cut(s) 2723
AccBSI CCGCTC 2 cut(s) 1957, 3445
AccI GTMKAC 1 cut(s) 1821
AccII CGCG 1 cut(s) 3327
AccIII TCCGGA 1 cut(s) 13
AciI CCGC 9 cut(s) 1359, 1592, 1731, 1955, 2330, 2813, 3445, 3459, 3463
AclI AACGTT 1 cut(s) 3405
AclWI GGATC 2 cut(s) 305, 3311
AcoI YGGCCR 3 cut(s) 1376, 2327, 3460
AcsI RAATTY 6 cut(s) 70, 167, 894, 1663, 2624, 2858
AcuI CTGAAG 3 cut(s) 431, 1319, 3240
AcyI GRCGYC 1 cut(s) 182
AdeI CACNNNGTG 1 cut(s) 2144
AfiI CCNNNNNNNGG 4 cut(s) 1381, 2290, 2669, 2849
AflIII ACRYGT 5 cut(s) 423, 1306, 2154, 2632, 3213
AjiI CACGTC 1 cut(s) 2633
AjnI CCWGG 8 cut(s) 96, 159, 467, 492, 1102, 2368, 3552, 3593
Alw21I GWGCWC 3 cut(s) 2226, 2454, 2529
Alw26I GTCTC 7 cut(s) 773, 1344, 1893, 2238, 2684, 2847, 3584
Alw44I GTGCAC 1 cut(s) 2450
AlwI GGATC 2 cut(s) 305, 3311
Ama87I CYCGRG 1 cut(s) 1425
Aor13HI TCCGGA 1 cut(s) 13
AoxI GGCC 9 cut(s) 208, 1219, 1376, 2088, 2327, 3184, 3248, 3460, 3537
ApaI GGGCCC 1 cut(s) 3188
ApaLI GTGCAC 1 cut(s) 2450
ApeKI GCWGC 6 cut(s) 347, 732, 1991, 3008, 3271, 3428
ApoI RAATTY 6 cut(s) 70, 167, 894, 1663, 2624, 2858
ArsI GACNNNNNNTTYG 8 cut(s) 86, 118, 326, 358, 650, 682, 2977, 3009
AseI ATTAAT 2 cut(s) 228, 2958
Asp700I GAANNNNTTC 2 cut(s) 710, 3162
Asp718I GGTACC 1 cut(s) 2723
AspA2I CCTAGG 1 cut(s) 1354
AspLEI GCGC 3 cut(s) 1681, 1923, 3327
AspS9I GGNCC 7 cut(s) 2088, 2887, 3184, 3185, 3248, 3476, 3537
AsuC2I CCSGG 6 cut(s) 826, 1634, 2492, 2991, 3268, 3488
AsuII TTCGAA 1 cut(s) 833
AvaI CYCGRG 1 cut(s) 1425
AvaII GGWCC 2 cut(s) 2887, 3476
AvrII CCTAGG 1 cut(s) 1354
BaeGI GKGCMC 2 cut(s) 2454, 3188
BanI GGYRCC 1 cut(s) 2723
BanII GRGCYC 3 cut(s) 1707, 2123, 3188
BbsI GAAGAC 3 cut(s) 713, 1125, 1547
Bbv12I GWGCWC 3 cut(s) 2226, 2454, 2529
BbvCI CCTCAGC 1 cut(s) 2547
BbvI GCAGC 6 cut(s) 359, 719, 2003, 2995, 3283, 3415
BccI CCATC 6 cut(s) 322, 1462, 1631, 2648, 3114, 3313
BceAI ACGGC 3 cut(s) 2061, 2342, 3218
BcgI CGANNNNNNTGC 2 cut(s) 1154, 1188
BciT130I CCWGG 8 cut(s) 98, 161, 469, 494, 1104, 2370, 3554, 3595
BciVI GTATCC 2 cut(s) 2287, 2366
BclI TGATCA 2 cut(s) 1363, 2206
BcnI CCSGG 6 cut(s) 826, 1634, 2492, 2991, 3268, 3488
BcoDI GTCTC 7 cut(s) 773, 1344, 1893, 2238, 2684, 2847, 3584
BfaI CTAG 7 cut(s) 441, 1181, 1286, 1355, 1394, 2865, 3002
BfmI CTRYAG 1 cut(s) 2478
BfuAI ACCTGC 1 cut(s) 1265
BfuI GTATCC 2 cut(s) 2287, 2366
BlnI CCTAGG 1 cut(s) 1354
BmcAI AGTACT 2 cut(s) 574, 2414
Bme18I GGWCC 2 cut(s) 2887, 3476
BmeT110I CYCGRG 1 cut(s) 1425
BmgBI CACGTC 1 cut(s) 2633
BmgT120I GGNCC 7 cut(s) 2088, 2887, 3184, 3185, 3248, 3476, 3537
BmiI GGNNCC 6 cut(s) 1704, 2725, 2888, 3186, 3477, 3538
BmrI ACTGGG 4 cut(s) 1615, 1813, 2372, 3255
BmuI ACTGGG 4 cut(s) 1615, 1813, 2372, 3255
BpiI GAAGAC 3 cut(s) 713, 1125, 1547
BpmI CTGGAG 3 cut(s) 1461, 1596, 2666
Bpu10I CCTNAGC 1 cut(s) 2547
Bpu14I TTCGAA 1 cut(s) 833
BpuEI CTTGAG 2 cut(s) 705, 3566
BpuMI CCSGG 6 cut(s) 826, 1634, 2492, 2991, 3268, 3488
BsaHI GRCGYC 1 cut(s) 182
BsaI GGTCTC 2 cut(s) 773, 1344
BsaWI WCCGGW 1 cut(s) 13
BsaXI ACNNNNNCTCC 2 cut(s) 2698, 2728
Bsc4I CCNNNNNNNGG 4 cut(s) 1381, 2290, 2669, 2849
BseAI TCCGGA 1 cut(s) 13
BseBI CCWGG 8 cut(s) 98, 161, 469, 494, 1104, 2370, 3554, 3595
BseGI GGATG 8 cut(s) 22, 477, 642, 1249, 1453, 1473, 1594, 1642
BseLI CCNNNNNNNGG 4 cut(s) 1381, 2290, 2669, 2849
BseRI GAGGAG 2 cut(s) 1754, 3382
BseSI GKGCMC 2 cut(s) 2454, 3188
BseX3I CGGCCG 2 cut(s) 2327, 3460
BseXI GCAGC 6 cut(s) 359, 719, 2003, 2995, 3283, 3415
BseYI CCCAGC 1 cut(s) 1772
BsgI GTGCAG 2 cut(s) 720, 2076
Bsh1236I CGCG 1 cut(s) 3327
Bsh1285I CGRYCG 2 cut(s) 2330, 3463
BshFI GGCC 9 cut(s) 210, 1221, 1378, 2090, 2329, 3186, 3250, 3462, 3539
BshNI GGYRCC 1 cut(s) 2723
BsiEI CGRYCG 2 cut(s) 2330, 3463
BsiHKAI GWGCWC 3 cut(s) 2226, 2454, 2529
BsiHKCI CYCGRG 1 cut(s) 1425
BsiSI CCGG 8 cut(s) 14, 825, 1375, 1634, 2491, 2990, 3268, 3487
BslFI GGGAC 4 cut(s) 599, 671, 2287, 2397
BslI CCNNNNNNNGG 4 cut(s) 1381, 2290, 2669, 2849
BsmAI GTCTC 7 cut(s) 773, 1344, 1893, 2238, 2684, 2847, 3584
BsmBI CGTCTC 1 cut(s) 1893
BsmFI GGGAC 4 cut(s) 599, 671, 2287, 2397
BsmI GAATGC 4 cut(s) 1430, 2434, 2969, 3430
BsnI GGCC 9 cut(s) 210, 1221, 1378, 2090, 2329, 3186, 3250, 3462, 3539
Bso31I GGTCTC 2 cut(s) 773, 1344
BsoBI CYCGRG 1 cut(s) 1425
Bsp119I TTCGAA 1 cut(s) 833
Bsp120I GGGCCC 1 cut(s) 3184
Bsp1286I GDGCHC 6 cut(s) 1707, 2123, 2226, 2454, 2529, 3188
Bsp13I TCCGGA 1 cut(s) 13
Bsp1407I TGTACA 1 cut(s) 3138
Bsp143I GATC 7 cut(s) 142, 310, 952, 1363, 2206, 2728, 3303
Bsp19I CCATGG 1 cut(s) 3343
BspACI CCGC 9 cut(s) 1359, 1592, 1731, 1955, 2330, 2813, 3445, 3459, 3463
BspANI GGCC 9 cut(s) 210, 1221, 1378, 2090, 2329, 3186, 3250, 3462, 3539
BspEI TCCGGA 1 cut(s) 13
BspFNI CGCG 1 cut(s) 3327
BspHI TCATGA 1 cut(s) 2203
BspLI GGNNCC 6 cut(s) 1704, 2725, 2888, 3186, 3477, 3538
BspMI ACCTGC 1 cut(s) 1265
BspPI GGATC 2 cut(s) 305, 3311
BspT104I TTCGAA 1 cut(s) 833
BspT107I GGYRCC 1 cut(s) 2723
BspTNI GGTCTC 2 cut(s) 773, 1344
BsrBI CCGCTC 2 cut(s) 1957, 3445
BsrGI TGTACA 1 cut(s) 3138
BssMI GATC 7 cut(s) 142, 310, 952, 1363, 2206, 2728, 3303
BssNI GRCGYC 1 cut(s) 182
BssT1I CCWWGG 7 cut(s) 431, 673, 922, 1354, 2359, 2455, 3343
Bst2UI CCWGG 8 cut(s) 98, 161, 469, 494, 1104, 2370, 3554, 3595
Bst4CI ACNGT 7 cut(s) 490, 662, 775, 999, 1649, 2144, 2393
Bst6I CTCTTC 2 cut(s) 734, 2061
BstACI GRCGYC 1 cut(s) 182
BstAPI GCANNNNNTGC 1 cut(s) 2438
BstAUI TGTACA 1 cut(s) 3138
BstBI TTCGAA 1 cut(s) 833
BstC8I GCNNGC 9 cut(s) 438, 982, 1174, 1506, 1550, 2253, 2800, 2870, 3170
BstDSI CCRYGG 1 cut(s) 3343
BstF5I GGATG 8 cut(s) 22, 477, 642, 1249, 1453, 1473, 1594, 1642
BstFNI CGCG 1 cut(s) 3327
BstHHI GCGC 3 cut(s) 1681, 1923, 3327
BstKTI GATC 7 cut(s) 145, 313, 955, 1366, 2209, 2731, 3306
BstMAI GTCTC 7 cut(s) 773, 1344, 1893, 2238, 2684, 2847, 3584
BstMBI GATC 7 cut(s) 142, 310, 952, 1363, 2206, 2728, 3303
BstMCI CGRYCG 2 cut(s) 2330, 3463
BstNI CCWGG 8 cut(s) 98, 161, 469, 494, 1104, 2370, 3554, 3595
BstNSI RCATGY 7 cut(s) 427, 1135, 1319, 2158, 2872, 2969, 3217
BstSFI CTRYAG 1 cut(s) 2478
BstSLI GKGCMC 2 cut(s) 2454, 3188
BstUI CGCG 1 cut(s) 3327
BstV1I GCAGC 6 cut(s) 359, 719, 2003, 2995, 3283, 3415
BstV2I GAAGAC 3 cut(s) 713, 1125, 1547
BstXI CCANNNNNNTGG 1 cut(s) 390
BstZI CGGCCG 2 cut(s) 2327, 3460
BsuI GTATCC 2 cut(s) 2287, 2366
BsuRI GGCC 9 cut(s) 210, 1221, 1378, 2090, 2329, 3186, 3250, 3462, 3539
BtgI CCRYGG 1 cut(s) 3343
BtrI CACGTC 1 cut(s) 2633
BtsCI GGATG 8 cut(s) 22, 477, 642, 1249, 1453, 1473, 1594, 1642
BtsI GCAGTG 1 cut(s) 1674
BveI ACCTGC 1 cut(s) 1265
Cac8I GCNNGC 9 cut(s) 438, 982, 1174, 1506, 1550, 2253, 2800, 2870, 3170
CciI TCATGA 1 cut(s) 2203
CciNI GCGGCCGC 1 cut(s) 3460
CfoI GCGC 3 cut(s) 1681, 1923, 3327
Cfr13I GGNCC 7 cut(s) 2088, 2887, 3184, 3185, 3248, 3476, 3537
CseI GACGC 2 cut(s) 171, 1869
CspCI CAANNNNNGTGG 2 cut(s) 1663, 1698
DpnI GATC 7 cut(s) 144, 312, 954, 1365, 2208, 2730, 3305
DpnII GATC 7 cut(s) 142, 310, 952, 1363, 2206, 2728, 3303
DraI TTTAAA 1 cut(s) 532
DraIII CACNNNGTG 1 cut(s) 2144
EaeI YGGCCR 3 cut(s) 1376, 2327, 3460
EagI CGGCCG 2 cut(s) 2327, 3460
Eam1104I CTCTTC 2 cut(s) 734, 2061
EarI CTCTTC 2 cut(s) 734, 2061
EclXI CGGCCG 2 cut(s) 2327, 3460
Eco130I CCWWGG 7 cut(s) 431, 673, 922, 1354, 2359, 2455, 3343
Eco24I GRGCYC 3 cut(s) 1707, 2123, 3188
Eco31I GGTCTC 2 cut(s) 773, 1344
Eco32I GATATC 3 cut(s) 1479, 1589, 2695
Eco47I GGWCC 2 cut(s) 2887, 3476
Eco52I CGGCCG 2 cut(s) 2327, 3460
Eco57I CTGAAG 3 cut(s) 431, 1319, 3240
Eco88I CYCGRG 1 cut(s) 1425
EcoO109I RGGNCCY 1 cut(s) 2887
EcoRI GAATTC 1 cut(s) 167
EcoRII CCWGG 8 cut(s) 96, 159, 467, 492, 1102, 2368, 3552, 3593
EcoRV GATATC 3 cut(s) 1479, 1589, 2695
EcoT14I CCWWGG 7 cut(s) 431, 673, 922, 1354, 2359, 2455, 3343
EcoT22I ATGCAT 1 cut(s) 2971
EcoT38I GRGCYC 3 cut(s) 1707, 2123, 3188
ErhI CCWWGG 7 cut(s) 431, 673, 922, 1354, 2359, 2455, 3343
Esp3I CGTCTC 1 cut(s) 1893
FaqI GGGAC 4 cut(s) 599, 671, 2287, 2397
FauNDI CATATG 1 cut(s) 3532
FbaI TGATCA 2 cut(s) 1363, 2206
FblI GTMKAC 1 cut(s) 1821
FokI GGATG 8 cut(s) 29, 484, 649, 1256, 1440, 1480, 1581, 1649
FriOI GRGCYC 3 cut(s) 1707, 2123, 3188
FspAI RTGCGCAY 1 cut(s) 1922
FspBI CTAG 7 cut(s) 441, 1181, 1286, 1355, 1394, 2865, 3002
FspI TGCGCA 1 cut(s) 1922
GlaI GCGC 3 cut(s) 1680, 1922, 3326
GsaI CCCAGC 1 cut(s) 1776
GsuI CTGGAG 3 cut(s) 1461, 1596, 2666
HaeIII GGCC 9 cut(s) 210, 1221, 1378, 2090, 2329, 3186, 3250, 3462, 3539
HapII CCGG 8 cut(s) 14, 825, 1375, 1634, 2491, 2990, 3268, 3487
HgaI GACGC 2 cut(s) 171, 1869
HhaI GCGC 3 cut(s) 1681, 1923, 3327
Hin1I GRCGYC 1 cut(s) 182
Hin6I GCGC 3 cut(s) 1679, 1921, 3325
HinP1I GCGC 3 cut(s) 1679, 1921, 3325
HincII GTYRAC 4 cut(s) 888, 2602, 2638, 2996
HindII GTYRAC 4 cut(s) 888, 2602, 2638, 2996
HindIII AAGCTT 1 cut(s) 836
HpaII CCGG 8 cut(s) 14, 825, 1375, 1634, 2491, 2990, 3268, 3487
Hpy99I CGWCG 1 cut(s) 1863
HpyCH4III ACNGT 7 cut(s) 490, 662, 775, 999, 1649, 2144, 2393
HpyCH4IV ACGT 8 cut(s) 458, 1308, 1951, 2297, 2506, 2632, 2836, 3405
HpySE526I ACGT 8 cut(s) 458, 1308, 1951, 2297, 2506, 2632, 2836, 3405
Hsp92I GRCGYC 1 cut(s) 182
HspAI GCGC 3 cut(s) 1679, 1921, 3325
Kpn2I TCCGGA 1 cut(s) 13
KpnI GGTACC 1 cut(s) 2727
Ksp22I TGATCA 2 cut(s) 1363, 2206
Kzo9I GATC 7 cut(s) 142, 310, 952, 1363, 2206, 2728, 3303
LmnI GCTCC 6 cut(s) 5, 1442, 1702, 3322, 3347, 3556
Lsp1109I GCAGC 6 cut(s) 359, 719, 2003, 2995, 3283, 3415
MaeI CTAG 7 cut(s) 441, 1181, 1286, 1355, 1394, 2865, 3002
MaeII ACGT 8 cut(s) 458, 1308, 1951, 2297, 2506, 2632, 2836, 3405
MalI GATC 7 cut(s) 144, 312, 954, 1365, 2208, 2730, 3305
MbiI CCGCTC 2 cut(s) 1957, 3445
MboI GATC 7 cut(s) 142, 310, 952, 1363, 2206, 2728, 3303
MfeI CAATTG 1 cut(s) 2433
MhlI GDGCHC 6 cut(s) 1707, 2123, 2226, 2454, 2529, 3188
MlyI GAGTC 5 cut(s) 559, 654, 1817, 3383, 3491
MmeI TCCRAC 1 cut(s) 614
Mph1103I ATGCAT 1 cut(s) 2971
MroI TCCGGA 1 cut(s) 13
MroXI GAANNNNTTC 2 cut(s) 710, 3162
MseI TTAA 8 cut(s) 228, 531, 1986, 2094, 2744, 2825, 2958, 3231
MslI CAYNNNNRTG 2 cut(s) 1130, 2208
MspA1I CMGCKG 1 cut(s) 3172
MspI CCGG 8 cut(s) 14, 825, 1375, 1634, 2491, 2990, 3268, 3487
MunI CAATTG 1 cut(s) 2433
Mva1269I GAATGC 4 cut(s) 1430, 2434, 2969, 3430
MvaI CCWGG 8 cut(s) 98, 161, 469, 494, 1104, 2370, 3554, 3595
MvnI CGCG 1 cut(s) 3327
NciI CCSGG 6 cut(s) 826, 1634, 2492, 2991, 3268, 3488
NcoI CCATGG 1 cut(s) 3343
NdeI CATATG 1 cut(s) 3532
NdeII GATC 7 cut(s) 142, 310, 952, 1363, 2206, 2728, 3303
NlaIV GGNNCC 6 cut(s) 1704, 2725, 2888, 3186, 3477, 3538
NmuCI GTSAC 5 cut(s) 367, 407, 521, 1799, 3386
NotI GCGGCCGC 1 cut(s) 3460
NsbI TGCGCA 1 cut(s) 1922
NsiI ATGCAT 1 cut(s) 2971
NspI RCATGY 7 cut(s) 427, 1135, 1319, 2158, 2872, 2969, 3217
NspV TTCGAA 1 cut(s) 833
PaeI GCATGC 1 cut(s) 2872
PagI TCATGA 1 cut(s) 2203
PaqCI CACCTGC 1 cut(s) 1265
PciI ACATGT 3 cut(s) 423, 2154, 3213
PctI GAATGC 4 cut(s) 1430, 2434, 2969, 3430
PdmI GAANNNNTTC 2 cut(s) 710, 3162
PfeI GAWTC 8 cut(s) 125, 275, 604, 1945, 2354, 2474, 2717, 3208
PfoI TCCNGGA 2 cut(s) 159, 824
PleI GAGTC 5 cut(s) 559, 653, 1817, 3383, 3491
PpsI GAGTC 5 cut(s) 559, 653, 1817, 3383, 3491
PpuMI RGGWCCY 1 cut(s) 2887
PscI ACATGT 3 cut(s) 423, 2154, 3213
PshBI ATTAAT 2 cut(s) 228, 2958
PsiI TTATAA 2 cut(s) 689, 2463
Psp1406I AACGTT 1 cut(s) 3405
Psp5II RGGWCCY 1 cut(s) 2887
Psp6I CCWGG 8 cut(s) 96, 159, 467, 492, 1102, 2368, 3552, 3593
PspFI CCCAGC 1 cut(s) 1772
PspGI CCWGG 8 cut(s) 96, 159, 467, 492, 1102, 2368, 3552, 3593
PspN4I GGNNCC 6 cut(s) 1704, 2725, 2888, 3186, 3477, 3538
PspOMI GGGCCC 1 cut(s) 3184
PspPI GGNCC 7 cut(s) 2088, 2887, 3184, 3185, 3248, 3476, 3537
PspPPI RGGWCCY 1 cut(s) 2887
PsrI GAACNNNNNNTAC 4 cut(s) 443, 475, 2503, 2535
PvuII CAGCTG 1 cut(s) 3172
RseI CAYNNNNRTG 2 cut(s) 1130, 2208
SaqAI TTAA 8 cut(s) 228, 531, 1986, 2094, 2744, 2825, 2958, 3231
Sau3AI GATC 7 cut(s) 142, 310, 952, 1363, 2206, 2728, 3303
Sau96I GGNCC 7 cut(s) 2088, 2887, 3184, 3185, 3248, 3476, 3537
ScaI AGTACT 2 cut(s) 574, 2414
SchI GAGTC 5 cut(s) 559, 654, 1817, 3383, 3491
SduI GDGCHC 6 cut(s) 1707, 2123, 2226, 2454, 2529, 3188
SfcI CTRYAG 1 cut(s) 2478
SfuI TTCGAA 1 cut(s) 833
SinI GGWCC 2 cut(s) 2887, 3476
SmiMI CAYNNNNRTG 2 cut(s) 1130, 2208
SmlI CTYRAG 2 cut(s) 720, 3581
SmoI CTYRAG 2 cut(s) 720, 3581
SphI GCATGC 1 cut(s) 2872
SsiI CCGC 9 cut(s) 1359, 1592, 1731, 1955, 2330, 2813, 3445, 3459, 3463
SspI AATATT 2 cut(s) 484, 1498
SspMI CTAG 7 cut(s) 441, 1181, 1286, 1355, 1394, 2865, 3002
StyI CCWWGG 7 cut(s) 431, 673, 922, 1354, 2359, 2455, 3343
TaaI ACNGT 7 cut(s) 490, 662, 775, 999, 1649, 2144, 2393
TaiI ACGT 8 cut(s) 461, 1311, 1954, 2300, 2509, 2635, 2839, 3408
TaqI TCGA 5 cut(s) 128, 516, 648, 833, 2028
TatI WGTACW 4 cut(s) 572, 2133, 2412, 3138
TauI GCSGC 4 cut(s) 1734, 2332, 3462, 3465
TfiI GAWTC 8 cut(s) 125, 275, 604, 1945, 2354, 2474, 2717, 3208
Tru1I TTAA 8 cut(s) 228, 531, 1986, 2094, 2744, 2825, 2958, 3231
Tru9I TTAA 8 cut(s) 228, 531, 1986, 2094, 2744, 2825, 2958, 3231
TseFI GTSAC 5 cut(s) 367, 407, 521, 1799, 3386
TseI GCWGC 6 cut(s) 347, 732, 1991, 3008, 3271, 3428
Tsp45I GTSAC 5 cut(s) 367, 407, 521, 1799, 3386
TspDTI ATGAA 9 cut(s) 126, 222, 653, 703, 2038, 2501, 3089, 3381, 3416
TspGWI ACGGA 3 cut(s) 39, 1001, 1336
VneI GTGCAC 1 cut(s) 2450
VpaK11BI GGWCC 2 cut(s) 2887, 3476
VspI ATTAAT 2 cut(s) 228, 2958
XapI RAATTY 6 cut(s) 70, 167, 894, 1663, 2624, 2858
XbaI TCTAGA 2 cut(s) 1285, 1393
XceI RCATGY 7 cut(s) 427, 1135, 1319, 2158, 2872, 2969, 3217
XcmI CCANNNNNNNNNTGG 1 cut(s) 2624
XmaJI CCTAGG 1 cut(s) 1354
XmiI GTMKAC 1 cut(s) 1821
XmnI GAANNNNTTC 2 cut(s) 710, 3162
XspI CTAG 7 cut(s) 441, 1181, 1286, 1355, 1394, 2865, 3002
ZrmI AGTACT 2 cut(s) 574, 2414
Zsp2I ATGCAT 1 cut(s) 2971
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.