RLG00000016333

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0002206
Physical Location & Seq
Reverse (-)
5719126 .. 5726374
7249 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016333

Sequence Viewer

Length: 3648 bp
ATGGGAGCGTCACCGGATGCCAACGGAGATATAGGTATGAGTAGCAAGGGAAAGGGCTTTTCCAATGGGGATATTGAGGACATGGATATTGACGACATGGATTTTGAGGATGATGAAGTTGATGATTCGACTAGCATTCATGATCTGGGAGAAGTGTTTCTCAGGGATTTCTGTAAGGAGGCATCAGTGTCATTCTTCAAGGAGTATGGCCTTCTCAGTCACCAGATAAACTCATACAATGACTTCGTTAAAAATGGCATACAAAGGGTCTTTGATTCTTTCGGTGAGATTGTAGTTGAGCCTGGGTATGACCCATCAAAGAAGGGAGATGCTGAGTGGCGATTTGCATCTGTCAAATTTGGGAAGGTCTCCCTCAACCGACCCTGTTTTTGGGGAGGTTCTGACAATGATAAGGAGTATAATATGTTACCCAGACATGCTAGGCTTCAGAACATGACATACTCAGCCAGGATGAAAGTCAATATTACGGTCCAGGTATATACTCAAAATCTTGTGAAAAGTGACAAGTTTAAAACTGGAAGAGAGCAATTTCTGGACAAGCAGACTGTGAGTACTGACAGCAGGGACATCATAATTGGTCGGGTTCCTGTAATGGTGAAGTCTGACTTGTGTTGGATGAAGGAAGTCGAGAAAGGTGACTGTGAGTTTGACCAGGGAGGCTACTTTATAATCAAGGGTGCAGAGAAGACATTTATCGCTCAAGAACAGAGCTGTTTGAAGAGACTTCTGATTACAAATAATCAGGGTCTCACAGTAGCATATAGGTCAGAGGTCAAGCGGCATAGGCTCATAATTAGATTATCCGGGATTTCGAAACTTGAAAATGTTGAAGGAGTGGAGAAAGTCCTGTCAGTGTACTTTATGTCAACAGAAATCCCTGTTTGGATTTGGTTTTTCGCCCTTGGGGTTAGTTCAGACAAAGAAGTTATCGATCTCATTGATTATGGCAAAGAAGATGCAAGCATTTCAAACATACTGTTTGCCTCAATTCGTGATGCTGACGAGAGAAAAGATGACTTTCGTAGGGGGAAGAATGCTCTGAGATATGTTGAAGGAGCGATAAAGAATACGACATTTCCACCTGGGGAAAGCATTGAGGACTGCATTAGCATGTATCTGTTTCCCAATCTGAGAGGCATGAAGCAGAAGGCTCGGTTTCTAGGGTATATGGTGAAGTGTCTTTTACAGACCTTCAGTGGCCGAAGGAAATGCGATAATAGGGATGACTTTAGAAACAAGAGGTTGGAGTTAGCAGGTGAGCTTCTAGAACGAGAGCTGAAGGCACATATTTCACATGCACGGAGGCGCATGGCAAAGGCTTTGCAGAGAGATCTATGTGGTGATCGTGTTGTCCGCCCGGTGGAGCACTATCTAGATGCATCTATAGTTACCAGTGGTGTTTCCAGAGCATTCTCTACTGGAGCATGGTGTCATCCTTTCAAGAGGATGGAGAGGATATCAGGTGTGGTCGCAAATGTTGGGCGAGCAAACCCACTGCAGACAATGATTGATATGAGGAAGACACGCCAGCAAGTTCAATACACTGGCAAGGCTGGAGATGCCAGATATCCGCATCCTTCTCACTGGGGTAAGGTTTGCTTTCTCTCAACCCCAGATGGTGAGAATTGTGGACTTGTAAAAAATTTGGCTACCACTGCTCTTGTAAGTACAAACATATTGGAGACCATATTGCCCAAATTGTTCCGATGTGGCATGGAGGAGTTAGTAGATGATACTTCTACTTCACTGGATGGGAAGCATAAAGTTTTTCTGAATGGTGACTGGGTTGGCGTTTGTGTAGATTCCCATGGTTTTGTCACGAAACTAAGGACATTGCGACGCGGGAAAAAGTTGCCACACGAGGTGGAAATCAAAAGAGATGAACACCAAGGAGAAGTGCGCATATTTTCTGATGCTGGAAGGATTCTACGTCCGCTCTTGGTAGTCCAGAATTTGAAGAAGATTAAAGCAGCAAAAGGAGAAAAACTTACCTTCAAAGATTTTCTTGACAAGGGAATAATTGAGCTCATTGGCCCGGAAGAAGAAGAGGACTGCCGTACAGCATGGGCCATTAAGTATCTGTTTATGGAAGAACAGGGAAACTCAGTTGTCAAGTACACACACTGTGAGCTGGACATGTCGTTTTTGTTGGGTTTAAGCTGTGGTATCATTCCCTTTGCAAATCATGATCATGCAAGGAGGGTGCTCTACCAGTCTCAGAAGCACTCTCAGCAGGCCATTGGTTTTTCTACTACAAATCCGAACCTCAGAGTGGATACTTTGTCTCACAACTTACACTACCCCCAGAGGCCGCTCTTTCGTACAATGATATCTGATTCTCTTGGAAAACCAGGATACCCGGTGGGACATAACGGTATCATGCCAAAGCCTGAGTACTTCAATGGCCAGAATGCAATTGTTGCAGTCAACGTGCACCTCGGATATAACCAAGAAGATTCAATAGTAATGAACCGGGCATCACTAGAACGTGGTATGTTTCGATCTGAGCACATAAGGAGTTACAAGGCTGAAGTTGAAAACAAGCTCTCAGTGGAAAAAAGACGAAAACCAGATGAGTGTGTAAATTTTGGCAAAATCCAGAGTAAATTTGGACGTGTTGACAGCCTTGATGATGATGGATTCCCCTGTGTTGGTGCCAATCTGCAGAATGGGGATATTATTATTGGGAGGTGCAGTGAATCTGGTACTGATCATAGCATCAAATTGAAACATACTGAAAGGGGAATGGTGCAGAAGGTTGTGCTTTCCTCTAATGATGATGGCAAGAATTTTGCTGTTGTATCATTAAGACAGGTTCGTTCTCCTTCTCTTGGAGACAAGTTCTCAAGCATGCATGGGCAAAAGGGTGTCCTAGGTTTTTTGGAGTCTCAAGAGAATTTCCCTTTCACAATACAAGGGATAGTTCCAGATATAGTAATTAATCCACATGCGTTTCCCTCAAGACAAACTCCCGGTCAACTCCTCGAGGCTGCTTTAGGGAAGGGGATTGCCTGTGGTGGTTCAAAGAGATACGCGACCCCTTTCTCTACTCTCTCTGTTGATGACATCACAGACCAGCTTCATAGAGCTGGATTTTCAAGATGGGGAAATGAGAGAGTATACAATGGTCGAACTGGTGAAATGGTTCGCCAACTGATATTCATGGGCCCAACATTCTATCAACGTCTGATTCACATGTCTGAAGACAAAGTAAAGTTTAGGAACACTGGGCCAGTTCACCCTCTCACCCGGCAGCCAGTGGCAGACAGGAAGCGATTTGGTGGGATCAAGTTTGGTGAGATGGAGCGCGACTGTCTTATAGCCCATGGAGCATCAGCAAACTTGCATGAACGTCTCTTCACTCTCAGTGACTCCTCCCTGATGAATGTTTGCCAGAAATGCCAGAATGCAGCTAATGTGATAGAGCGGAAAGTCACTGGTGGCCGCAAAATCAGGGGTCCCTATTGCCGGGTCTGCGACTCTGCTGATGACATTGTGAGGGTGAATGTGCCATACGGGGCCAAGCTATTGTGCCAGGAGCTATTCAGCATGGGCATCAGTCTCAAGTTTGAAACCAGGCTCTACTCGCCGTTTGATCACCACCTTCTTCATCCTTTCGGACTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000228 GO:0000418 GO:0000419 GO:0000428 GO:0000785 GO:0000790 GO:0000792 GO:0002682 GO:0003674 GO:0003824 GO:0003899 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005720 GO:0005730 GO:0005736 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009605 GO:0009607 GO:0009620 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016020 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019222 GO:0019438 GO:0030422 GO:0030880 GO:0031047 GO:0031050 GO:0031974 GO:0031981 GO:0032259 GO:0032774 GO:0032991 GO:0034062 GO:0034641 GO:0034654 GO:0035194 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043331 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044451 GO:0044452 GO:0044454 GO:0044464 GO:0044728 GO:0046483 GO:0048519 GO:0048583 GO:0050776 GO:0050789 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0055029 GO:0060255 GO:0061695 GO:0065007 GO:0070013 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0097747 GO:0098542 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1901698 GO:1901699 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko01100 ko03020 ko05016 ko05169 map00230 map00240 map01100 map03020 map05016 map05169
Pfam Domains
Protein Families
RNA_pol_Rpb2_1 RNA_pol_Rpb2_2 RNA_pol_Rpb2_3 RNA_pol_Rpb2_4 RNA_pol_Rpb2_5 RNA_pol_Rpb2_6 RNA_pol_Rpb2_7

Protein Analysis

1216

Amino Acids

137.1

Weight (kDa)

8.69

Isoelectric Point (pI)

36.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNA_pol_Rpb2_1 PF04563 70 - 453 4.1e-35 RNA polymerase beta subunit
RNA_pol_Rpb2_2 PF04561 284 - 423 7e-12 RNA polymerase Rpb2, domain 2
RNA_pol_Rpb2_3 PF04565 502 - 561 6.3e-15 RNA polymerase Rpb2, domain 3
RNA_pol_Rpb2_4 PF04566 596 - 657 8.8e-23 RNA polymerase Rpb2, domain 4
RNA_pol_Rpb2_5 PF04567 672 - 719 3.5e-06 RNA polymerase Rpb2, domain 5
RNA_pol_Rpb2_6 PF00562 728 - 1099 6.2e-111 RNA polymerase Rpb2, domain 6
RNA_pol_Rpb2_7 PF04560 1101 - 1198 2.8e-26 RNA polymerase Rpb2, domain 7
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 689
AarI CACCTGC 1 cut(s) 1265
AbsI CCTCGAGG 1 cut(s) 3005
Acc16I TGCGCA 1 cut(s) 1922
Acc36I ACCTGC 1 cut(s) 1265
AccB1I GGYRCC 1 cut(s) 2675
AccBSI CCGCTC 3 cut(s) 1957, 2335, 3448
AccI GTMKAC 1 cut(s) 3141
AccII CGCG 3 cut(s) 1863, 3056, 3330
AciI CCGC 8 cut(s) 799, 1375, 1592, 1863, 1955, 2333, 3448, 3466
AclWI GGATC 1 cut(s) 3314
AcoI YGGCCR 3 cut(s) 1219, 2425, 3463
AcsI RAATTY 7 cut(s) 356, 1663, 1972, 2605, 2627, 2809, 2917
AcuI CTGAAG 5 cut(s) 431, 1198, 1319, 2571, 3243
AdeI CACNNNGTG 2 cut(s) 1885, 2147
AfaI GTAC 8 cut(s) 574, 878, 1690, 2080, 2138, 2344, 2417, 2728
AfiI CCNNNNNNNGG 6 cut(s) 390, 1381, 2293, 2672, 2852, 3489
AflIII ACRYGT 3 cut(s) 2157, 2635, 3216
AjiI CACGTC 1 cut(s) 2636
AjnI CCWGG 8 cut(s) 301, 467, 492, 672, 1102, 2371, 3555, 3596
Alw21I GWGCWC 5 cut(s) 1389, 2049, 2229, 2457, 2532
Alw44I GTGCAC 1 cut(s) 2453
AlwI GGATC 1 cut(s) 3314
Ama87I CYCGRG 1 cut(s) 3005
ApaI GGGCCC 1 cut(s) 3191
ApaLI GTGCAC 1 cut(s) 2453
ApeKI GCWGC 4 cut(s) 1991, 3011, 3274, 3431
ApoI RAATTY 7 cut(s) 356, 1663, 1972, 2605, 2627, 2809, 2917
ArsI GACNNNNNNTTYG 8 cut(s) 86, 118, 372, 404, 650, 682, 2980, 3012
AseI ATTAAT 1 cut(s) 2961
Asp700I GAANNNNTTC 2 cut(s) 156, 3165
AspA2I CCTAGG 1 cut(s) 2893
AspLEI GCGC 3 cut(s) 1329, 1923, 3330
AspS9I GGNCC 8 cut(s) 490, 2054, 2088, 3187, 3188, 3251, 3479, 3540
AsuC2I CCSGG 8 cut(s) 826, 1379, 2057, 2381, 2495, 2994, 3271, 3491
AsuII TTCGAA 1 cut(s) 833
AvaI CYCGRG 1 cut(s) 3005
AvaII GGWCC 2 cut(s) 490, 3479
AvrII CCTAGG 1 cut(s) 2893
BaeGI GKGCMC 2 cut(s) 2457, 3191
BalI TGGCCA 1 cut(s) 2427
BanI GGYRCC 1 cut(s) 2675
BanII GRGCYC 2 cut(s) 2049, 3191
BauI CACGAG 1 cut(s) 1880
BbsI GAAGAC 3 cut(s) 713, 1547, 3231
Bbv12I GWGCWC 5 cut(s) 1389, 2049, 2229, 2457, 2532
BbvI GCAGC 4 cut(s) 2003, 2998, 3286, 3443
BccI CCATC 8 cut(s) 322, 1462, 1631, 1766, 2651, 2795, 3117, 3316
BceAI ACGGC 2 cut(s) 2061, 3595
BcgI CGANNNNNNTGC 4 cut(s) 1154, 1188, 1212, 1246
BciT130I CCWGG 8 cut(s) 303, 469, 494, 674, 1104, 2373, 3557, 3598
BciVI GTATCC 2 cut(s) 2290, 2369
BclI TGATCA 3 cut(s) 2209, 2731, 3616
BcnI CCSGG 8 cut(s) 826, 1379, 2057, 2381, 2495, 2994, 3271, 3491
BfaI CTAG 7 cut(s) 132, 441, 1181, 1286, 1394, 2504, 2894
BfmI CTRYAG 3 cut(s) 1404, 1517, 2684
BfuAI ACCTGC 1 cut(s) 1265
BfuI GTATCC 2 cut(s) 2290, 2369
BglII AGATCT 1 cut(s) 1351
BisI GCNGC 7 cut(s) 800, 1992, 2333, 3012, 3275, 3432, 3466
BlnI CCTAGG 1 cut(s) 2893
BlsI GCNGC 7 cut(s) 801, 1993, 2334, 3013, 3276, 3433, 3467
BmcAI AGTACT 2 cut(s) 574, 2417
Bme18I GGWCC 2 cut(s) 490, 3479
BmeT110I CYCGRG 1 cut(s) 3005
BmgBI CACGTC 1 cut(s) 2636
BmgT120I GGNCC 8 cut(s) 490, 2054, 2088, 3187, 3188, 3251, 3479, 3540
BmiI GGNNCC 6 cut(s) 606, 2677, 3189, 3480, 3481, 3541
BmrI ACTGGG 3 cut(s) 1615, 1813, 3258
BmuI ACTGGG 3 cut(s) 1615, 1813, 3258
BpiI GAAGAC 3 cut(s) 713, 1547, 3231
BpmI CTGGAG 2 cut(s) 1461, 1596
Bpu14I TTCGAA 1 cut(s) 833
BpuEI CTTGAG 5 cut(s) 705, 2851, 2895, 2965, 3569
BpuMI CCSGG 8 cut(s) 826, 1379, 2057, 2381, 2495, 2994, 3271, 3491
Bsa29I ATCGAT 1 cut(s) 951
BsaBI GATNNNNATC 1 cut(s) 346
BsaI GGTCTC 3 cut(s) 373, 773, 1697
BsaJI CCNNGG 9 cut(s) 302, 673, 922, 1103, 1828, 1909, 2458, 2893, 3346
BsaWI WCCGGW 1 cut(s) 13
BsaXI ACNNNNNCTCC 2 cut(s) 2701, 2731
Bsc4I CCNNNNNNNGG 6 cut(s) 390, 1381, 2293, 2672, 2852, 3489
Bse3DI GCAATG 1 cut(s) 1853
Bse8I GATNNNNATC 1 cut(s) 346
BseBI CCWGG 8 cut(s) 303, 469, 494, 674, 1104, 2373, 3557, 3598
BseCI ATCGAT 1 cut(s) 951
BseDI CCNNGG 9 cut(s) 302, 673, 922, 1103, 1828, 1909, 2458, 2893, 3346
BseJI GATNNNNATC 1 cut(s) 346
BseLI CCNNNNNNNGG 6 cut(s) 390, 1381, 2293, 2672, 2852, 3489
BseMI GCAATG 1 cut(s) 1853
BseRI GAGGAG 3 cut(s) 1754, 2993, 3385
BseSI GKGCMC 2 cut(s) 2457, 3191
BseXI GCAGC 4 cut(s) 2003, 2998, 3286, 3443
BsgI GTGCAG 3 cut(s) 720, 2734, 2792
Bsh1236I CGCG 3 cut(s) 1863, 3056, 3330
BshNI GGYRCC 1 cut(s) 2675
BshVI ATCGAT 1 cut(s) 951
BsiHKAI GWGCWC 5 cut(s) 1389, 2049, 2229, 2457, 2532
BsiHKCI CYCGRG 1 cut(s) 3005
BsiSI CCGG 9 cut(s) 14, 825, 1379, 2057, 2381, 2494, 2994, 3271, 3490
BslFI GGGAC 3 cut(s) 599, 2400, 3465
BslI CCNNNNNNNGG 6 cut(s) 390, 1381, 2293, 2672, 2852, 3489
BsmBI CGTCTC 1 cut(s) 3380
BsmFI GGGAC 3 cut(s) 599, 2400, 3465
BsmI GAATGC 5 cut(s) 135, 1060, 1430, 2437, 3433
Bso31I GGTCTC 3 cut(s) 373, 773, 1697
BsoBI CYCGRG 1 cut(s) 3005
Bsp119I TTCGAA 1 cut(s) 833
Bsp120I GGGCCC 1 cut(s) 3187
Bsp1286I GDGCHC 6 cut(s) 1389, 2049, 2229, 2457, 2532, 3191
Bsp143I GATC 9 cut(s) 142, 952, 1351, 1363, 2209, 2522, 2731, 3306, 3616
Bsp19I CCATGG 2 cut(s) 1828, 3346
BspACI CCGC 8 cut(s) 799, 1375, 1592, 1863, 1955, 2333, 3448, 3466
BspDI ATCGAT 1 cut(s) 951
BspFNI CGCG 3 cut(s) 1863, 3056, 3330
BspHI TCATGA 2 cut(s) 139, 2206
BspLI GGNNCC 6 cut(s) 606, 2677, 3189, 3480, 3481, 3541
BspMAI CTGCAG 2 cut(s) 1521, 2688
BspMI ACCTGC 1 cut(s) 1265
BspPI GGATC 1 cut(s) 3314
BspT104I TTCGAA 1 cut(s) 833
BspT107I GGYRCC 1 cut(s) 2675
BspTNI GGTCTC 3 cut(s) 373, 773, 1697
BsrBI CCGCTC 3 cut(s) 1957, 2335, 3448
BsrDI GCAATG 1 cut(s) 1853
BssECI CCNNGG 9 cut(s) 302, 673, 922, 1103, 1828, 1909, 2458, 2893, 3346
BssMI GATC 9 cut(s) 142, 952, 1351, 1363, 2209, 2522, 2731, 3306, 3616
BssNAI GTATAC 1 cut(s) 3142
BssSI CACGAG 1 cut(s) 1880
BssT1I CCWWGG 5 cut(s) 922, 1828, 1909, 2893, 3346
Bst1107I GTATAC 1 cut(s) 3142
Bst2BI CACGAG 1 cut(s) 1880
Bst2UI CCWGG 8 cut(s) 303, 469, 494, 674, 1104, 2373, 3557, 3598
Bst4CI ACNGT 9 cut(s) 490, 568, 662, 775, 999, 2147, 2396, 3335, 3645
Bst6I CTCTTC 4 cut(s) 535, 734, 2061, 3383
BstAPI GCANNNNNTGC 1 cut(s) 2441
BstBI TTCGAA 1 cut(s) 833
BstC8I GCNNGC 5 cut(s) 982, 1506, 1550, 2256, 2873
BstDSI CCRYGG 2 cut(s) 1828, 3346
BstFNI CGCG 3 cut(s) 1863, 3056, 3330
BstHHI GCGC 3 cut(s) 1329, 1923, 3330
BstKTI GATC 9 cut(s) 145, 955, 1354, 1366, 2212, 2525, 2734, 3309, 3619
BstMBI GATC 9 cut(s) 142, 952, 1351, 1363, 2209, 2522, 2731, 3306, 3616
BstMWI GCNNNNNNNGC 9 cut(s) 805, 1580, 1676, 2251, 2441, 3350, 3420, 3495, 3607
BstNI CCWGG 8 cut(s) 303, 469, 494, 674, 1104, 2373, 3557, 3598
BstNSI RCATGY 7 cut(s) 440, 1135, 1319, 2161, 2875, 2972, 3220
BstSFI CTRYAG 3 cut(s) 1404, 1517, 2684
BstSLI GKGCMC 2 cut(s) 2457, 3191
BstUI CGCG 3 cut(s) 1863, 3056, 3330
BstV1I GCAGC 4 cut(s) 2003, 2998, 3286, 3443
BstV2I GAAGAC 3 cut(s) 713, 1547, 3231
BstX2I RGATCY 1 cut(s) 1351
BstYI RGATCY 1 cut(s) 1351
BstZ17I GTATAC 1 cut(s) 3142
Bsu15I ATCGAT 1 cut(s) 951
BsuI GTATCC 2 cut(s) 2290, 2369
BsuTUI ATCGAT 1 cut(s) 951
BtgI CCRYGG 2 cut(s) 1828, 3346
BtrI CACGTC 1 cut(s) 2636
BtsI GCAGTG 3 cut(s) 1514, 1674, 2722
BveI ACCTGC 1 cut(s) 1265
Cac8I GCNNGC 5 cut(s) 982, 1506, 1550, 2256, 2873
CciI TCATGA 2 cut(s) 139, 2206
CfoI GCGC 3 cut(s) 1329, 1923, 3330
Cfr13I GGNCC 8 cut(s) 490, 2054, 2088, 3187, 3188, 3251, 3479, 3540
ClaI ATCGAT 1 cut(s) 951
CseI GACGC 1 cut(s) 1869
Csp6I GTAC 8 cut(s) 573, 877, 1689, 2079, 2137, 2343, 2416, 2727
CspCI CAANNNNNGTGG 2 cut(s) 1663, 1698
CviQI GTAC 8 cut(s) 573, 877, 1689, 2079, 2137, 2343, 2416, 2727
DpnI GATC 9 cut(s) 144, 954, 1353, 1365, 2211, 2524, 2733, 3308, 3618
DpnII GATC 9 cut(s) 142, 952, 1351, 1363, 2209, 2522, 2731, 3306, 3616
DraI TTTAAA 1 cut(s) 532
DraIII CACNNNGTG 2 cut(s) 1885, 2147
EaeI YGGCCR 3 cut(s) 1219, 2425, 3463
Eam1104I CTCTTC 4 cut(s) 535, 734, 2061, 3383
EarI CTCTTC 4 cut(s) 535, 734, 2061, 3383
EciI GGCGGA 1 cut(s) 1364
Ecl136II GAGCTC 1 cut(s) 2047
Eco130I CCWWGG 5 cut(s) 922, 1828, 1909, 2893, 3346
Eco24I GRGCYC 2 cut(s) 2049, 3191
Eco31I GGTCTC 3 cut(s) 373, 773, 1697
Eco32I GATATC 3 cut(s) 1479, 1589, 2352
Eco47I GGWCC 2 cut(s) 490, 3479
Eco53kI GAGCTC 1 cut(s) 2047
Eco57I CTGAAG 5 cut(s) 431, 1198, 1319, 2571, 3243
Eco88I CYCGRG 1 cut(s) 3005
EcoICRI GAGCTC 1 cut(s) 2047
EcoO109I RGGNCCY 1 cut(s) 3479
EcoRII CCWGG 8 cut(s) 301, 467, 492, 672, 1102, 2371, 3555, 3596
EcoRV GATATC 3 cut(s) 1479, 1589, 2352
EcoT14I CCWWGG 5 cut(s) 922, 1828, 1909, 2893, 3346
EcoT22I ATGCAT 2 cut(s) 1402, 2877
EcoT38I GRGCYC 2 cut(s) 2049, 3191
ErhI CCWWGG 5 cut(s) 922, 1828, 1909, 2893, 3346
Esp3I CGTCTC 1 cut(s) 3380
FalI AAGNNNNNCTT 6 cut(s) 611, 643, 1604, 1636, 2010, 2042
FaqI GGGAC 3 cut(s) 599, 2400, 3465
FauI CCCGC 1 cut(s) 1856
FbaI TGATCA 3 cut(s) 2209, 2731, 3616
FblI GTMKAC 1 cut(s) 3141
Fnu4HI GCNGC 7 cut(s) 800, 1992, 2333, 3012, 3275, 3432, 3466
FriOI GRGCYC 2 cut(s) 2049, 3191
Fsp4HI GCNGC 7 cut(s) 800, 1992, 2333, 3012, 3275, 3432, 3466
FspAI RTGCGCAY 1 cut(s) 1922
FspBI CTAG 7 cut(s) 132, 441, 1181, 1286, 1394, 2504, 2894
FspI TGCGCA 1 cut(s) 1922
GlaI GCGC 3 cut(s) 1328, 1922, 3329
GluI GCNGC 7 cut(s) 800, 1992, 2333, 3012, 3275, 3432, 3466
GsuI CTGGAG 2 cut(s) 1461, 1596
HapII CCGG 9 cut(s) 14, 825, 1379, 2057, 2381, 2494, 2994, 3271, 3490
HgaI GACGC 1 cut(s) 1869
HhaI GCGC 3 cut(s) 1329, 1923, 3330
Hin6I GCGC 3 cut(s) 1327, 1921, 3328
HinP1I GCGC 3 cut(s) 1327, 1921, 3328
HincII GTYRAC 4 cut(s) 888, 2449, 2641, 2999
HindII GTYRAC 4 cut(s) 888, 2449, 2641, 2999
HpaII CCGG 9 cut(s) 14, 825, 1379, 2057, 2381, 2494, 2994, 3271, 3490
Hpy99I CGWCG 1 cut(s) 1863
HpyCH4III ACNGT 9 cut(s) 490, 568, 662, 775, 999, 2147, 2396, 3335, 3645
HpyCH4IV ACGT 6 cut(s) 1951, 2451, 2509, 2635, 3205, 3373
HpyF10VI GCNNNNNNNGC 9 cut(s) 805, 1580, 1676, 2251, 2441, 3350, 3420, 3495, 3607
HpySE526I ACGT 6 cut(s) 1951, 2451, 2509, 2635, 3205, 3373
HspAI GCGC 3 cut(s) 1327, 1921, 3328
KflI GGGWCCC 1 cut(s) 3479
Ksp22I TGATCA 3 cut(s) 2209, 2731, 3616
Kzo9I GATC 9 cut(s) 142, 952, 1351, 1363, 2209, 2522, 2731, 3306, 3616
LmnI GCTCC 7 cut(s) 5, 1076, 1384, 1442, 3325, 3350, 3559
Lsp1109I GCAGC 4 cut(s) 2003, 2998, 3286, 3443
MaeI CTAG 7 cut(s) 132, 441, 1181, 1286, 1394, 2504, 2894
MaeII ACGT 6 cut(s) 1951, 2451, 2509, 2635, 3205, 3373
MalI GATC 9 cut(s) 144, 954, 1353, 1365, 2211, 2524, 2733, 3308, 3618
MbiI CCGCTC 3 cut(s) 1957, 2335, 3448
MboI GATC 9 cut(s) 142, 952, 1351, 1363, 2209, 2522, 2731, 3306, 3616
MfeI CAATTG 1 cut(s) 2436
MflI RGATCY 1 cut(s) 1351
MhlI GDGCHC 6 cut(s) 1389, 2049, 2229, 2457, 2532, 3191
MlsI TGGCCA 1 cut(s) 2427
MluNI TGGCCA 1 cut(s) 2427
MlyI GAGTC 3 cut(s) 2915, 3386, 3494
MmeI TCCRAC 2 cut(s) 614, 1245
Mox20I TGGCCA 1 cut(s) 2427
Mph1103I ATGCAT 2 cut(s) 1402, 2877
MroXI GAANNNNTTC 2 cut(s) 156, 3165
MscI TGGCCA 1 cut(s) 2427
MseI TTAA 7 cut(s) 249, 531, 1986, 2094, 2177, 2828, 2961
MslI CAYNNNNRTG 2 cut(s) 1130, 2211
Msp20I TGGCCA 1 cut(s) 2427
MspI CCGG 9 cut(s) 14, 825, 1379, 2057, 2381, 2494, 2994, 3271, 3490
MunI CAATTG 1 cut(s) 2436
Mva1269I GAATGC 5 cut(s) 135, 1060, 1430, 2437, 3433
MvaI CCWGG 8 cut(s) 303, 469, 494, 674, 1104, 2373, 3557, 3598
MvnI CGCG 3 cut(s) 1863, 3056, 3330
MwoI GCNNNNNNNGC 9 cut(s) 805, 1580, 1676, 2251, 2441, 3350, 3420, 3495, 3607
NciI CCSGG 8 cut(s) 826, 1379, 2057, 2381, 2495, 2994, 3271, 3491
NcoI CCATGG 2 cut(s) 1828, 3346
NdeII GATC 9 cut(s) 142, 952, 1351, 1363, 2209, 2522, 2731, 3306, 3616
NlaIV GGNNCC 6 cut(s) 606, 2677, 3189, 3480, 3481, 3541
NmuCI GTSAC 8 cut(s) 9, 218, 521, 656, 1799, 1837, 3389, 3454
NsbI TGCGCA 1 cut(s) 1922
NsiI ATGCAT 2 cut(s) 1402, 2877
NspI RCATGY 7 cut(s) 440, 1135, 1319, 2161, 2875, 2972, 3220
NspV TTCGAA 1 cut(s) 833
PaeI GCATGC 1 cut(s) 2875
PaeR7I CTCGAG 1 cut(s) 3005
PagI TCATGA 2 cut(s) 139, 2206
PaqCI CACCTGC 1 cut(s) 1265
PciI ACATGT 2 cut(s) 2157, 3216
PctI GAATGC 5 cut(s) 135, 1060, 1430, 2437, 3433
PdmI GAANNNNTTC 2 cut(s) 156, 3165
PfeI GAWTC 9 cut(s) 125, 275, 1823, 1945, 2357, 2477, 2661, 2720, 3211
PfoI TCCNGGA 1 cut(s) 824
PkrI GCNGC 7 cut(s) 801, 1993, 2334, 3013, 3276, 3433, 3467
PleI GAGTC 3 cut(s) 2914, 3386, 3494
PpsI GAGTC 3 cut(s) 2914, 3386, 3494
PpuMI RGGWCCY 1 cut(s) 3479
PscI ACATGT 2 cut(s) 2157, 3216
PshBI ATTAAT 1 cut(s) 2961
PsiI TTATAA 1 cut(s) 689
Psp124BI GAGCTC 1 cut(s) 2049
Psp5II RGGWCCY 1 cut(s) 3479
Psp6I CCWGG 8 cut(s) 301, 467, 492, 672, 1102, 2371, 3555, 3596
PspGI CCWGG 8 cut(s) 301, 467, 492, 672, 1102, 2371, 3555, 3596
PspN4I GGNNCC 6 cut(s) 606, 2677, 3189, 3480, 3481, 3541
PspOMI GGGCCC 1 cut(s) 3187
PspPI GGNCC 8 cut(s) 490, 2054, 2088, 3187, 3188, 3251, 3479, 3540
PspPPI RGGWCCY 1 cut(s) 3479
PspXI VCTCGAGB 1 cut(s) 3005
PsrI GAACNNNNNNTAC 2 cut(s) 443, 475
PstI CTGCAG 2 cut(s) 1521, 2688
PsuI RGATCY 1 cut(s) 1351
RsaI GTAC 8 cut(s) 574, 878, 1690, 2080, 2138, 2344, 2417, 2728
RsaNI GTAC 8 cut(s) 573, 877, 1689, 2079, 2137, 2343, 2416, 2727
RseI CAYNNNNRTG 2 cut(s) 1130, 2211
SacI GAGCTC 1 cut(s) 2049
SaqAI TTAA 7 cut(s) 249, 531, 1986, 2094, 2177, 2828, 2961
SatI GCNGC 7 cut(s) 800, 1992, 2333, 3012, 3275, 3432, 3466
Sau3AI GATC 9 cut(s) 142, 952, 1351, 1363, 2209, 2522, 2731, 3306, 3616
Sau96I GGNCC 8 cut(s) 490, 2054, 2088, 3187, 3188, 3251, 3479, 3540
ScaI AGTACT 2 cut(s) 574, 2417
SchI GAGTC 3 cut(s) 2915, 3386, 3494
SduI GDGCHC 6 cut(s) 1389, 2049, 2229, 2457, 2532, 3191
SfcI CTRYAG 3 cut(s) 1404, 1517, 2684
Sfr274I CTCGAG 1 cut(s) 3005
SfuI TTCGAA 1 cut(s) 833
SinI GGWCC 2 cut(s) 490, 3479
SlaI CTCGAG 1 cut(s) 3005
SmiMI CAYNNNNRTG 2 cut(s) 1130, 2211
SmlI CTYRAG 6 cut(s) 720, 2866, 2910, 2980, 3005, 3584
SmoI CTYRAG 6 cut(s) 720, 2866, 2910, 2980, 3005, 3584
SphI GCATGC 1 cut(s) 2875
SsiI CCGC 8 cut(s) 799, 1375, 1592, 1863, 1955, 2333, 3448, 3466
SspI AATATT 1 cut(s) 484
SspMI CTAG 7 cut(s) 132, 441, 1181, 1286, 1394, 2504, 2894
SstI GAGCTC 1 cut(s) 2049
StyI CCWWGG 5 cut(s) 922, 1828, 1909, 2893, 3346
TaaI ACNGT 9 cut(s) 490, 568, 662, 775, 999, 2147, 2396, 3335, 3645
TaiI ACGT 6 cut(s) 1954, 2454, 2512, 2638, 3208, 3376
TaqI TCGA 7 cut(s) 128, 648, 833, 951, 2521, 3006, 3151
TatI WGTACW 5 cut(s) 572, 876, 1688, 2136, 2415
TauI GCSGC 3 cut(s) 802, 2335, 3468
TfiI GAWTC 9 cut(s) 125, 275, 1823, 1945, 2357, 2477, 2661, 2720, 3211
Tru1I TTAA 7 cut(s) 249, 531, 1986, 2094, 2177, 2828, 2961
Tru9I TTAA 7 cut(s) 249, 531, 1986, 2094, 2177, 2828, 2961
TseFI GTSAC 8 cut(s) 9, 218, 521, 656, 1799, 1837, 3389, 3454
TseI GCWGC 4 cut(s) 1991, 3011, 3274, 3431
Tsp45I GTSAC 8 cut(s) 9, 218, 521, 656, 1799, 1837, 3389, 3454
TspGWI ACGGA 2 cut(s) 39, 1336
VneI GTGCAC 1 cut(s) 2453
VpaK11BI GGWCC 2 cut(s) 490, 3479
VspI ATTAAT 1 cut(s) 2961
XapI RAATTY 7 cut(s) 356, 1663, 1972, 2605, 2627, 2809, 2917
XbaI TCTAGA 2 cut(s) 1285, 1393
XceI RCATGY 7 cut(s) 440, 1135, 1319, 2161, 2875, 2972, 3220
XcmI CCANNNNNNNNNTGG 1 cut(s) 2627
XhoI CTCGAG 1 cut(s) 3005
XmaJI CCTAGG 1 cut(s) 2893
XmiI GTMKAC 1 cut(s) 3141
XmnI GAANNNNTTC 2 cut(s) 156, 3165
XspI CTAG 7 cut(s) 132, 441, 1181, 1286, 1394, 2504, 2894
ZrmI AGTACT 2 cut(s) 574, 2417
Zsp2I ATGCAT 2 cut(s) 1402, 2877
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.