Rh5DG299300

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0002206
Physical Location & Seq
Reverse (-)
37602498 .. 37614516
12019 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG299300.1

Sequence Viewer

Length: 4479 bp
ATGATAAAGAATACCGCGTTTCCTCCTCGGGAAAGCATTGAAGAATGCATCAGCATGTATCTGTTCCCCAATCTCAGGGGCTTGAAGCAAAAGGCTCGGTTTCTTGGCTATATGGTGAAGTCTCTTTTACAGACCTTCAGAGGCCACAGGAAATGTGACAACAGAGAAGAATTGAAGAACAAGAGGTTTCACTTGGCTGGTGAGCTTCTAGAGCGAGAGCTGAAGGCACACGTAGGACATGCAGGAAGGCGCATGGCAAAGGCTTTGCAGAGAGACCTTTGTGGTGACCGTGTTGTCTGCCTGGTTGAGCAATATCTAGATGCTACAGTAGTAACCAATGGCATCTCCAGAGCATTCTCAACTGGAGCGTGGTGTCATCCTTTCAAGAGGATGGAGAGGATGTCTGGTGTGGTGGTAAATGTTGGGAAAACAAATCCACTGCAAACAATGGTAGATATGAGGAAGACACGCCTCCAAGTTCAATACACGGGGAAGGTCGGAGATGCCAGATATCCGCATCCTTCTCACTGGGGTAGGGTTTGCTTTATCTCAACCCCAGATGGTGAGAATTGTGGGCTTGTGAAAAATTTGGCTACCACTGCACTGGTAAGTACAAACATATTGGAGCCCATATTGCCCGACTTTTTCTCTAGTGGCATGGAGGAGCTGGTAGATGATACTTCAACATCACTTCATGGGAAGAATAAAGTTTTTCTCAATGGGGATTGGGTTGGTGTTTGTGAAGATTCCCATGATTTTGTCATGAAACTAAGGACATTGAGACGCAGGAAAAAGTTGCTACATCAGGTGGAAATCAAAAGAGATGAGCAGCTCCTGGAAGTACGCATATATTCTGATGCTGGAAGGATTCTACGTCCTTTCCTGGTAGTTAAGAATTTGAATAAGATTAAAGAATCAAAAGGGAAAAAACACACGTTCCAGGATCTTCTTGGCAATGGAATAATCGAGCTCATTGGAGCTGAAGAAGAAGAGGATTGCTGTACAGCATGGGACGTCAAGCATCTATTTATGGAGGGGAAGTCAGCAGTCAAGTACACACACTGTGAACTGGACCTATCATTTTTGTTAGGCTTAAGTTGTGGTATCATTCCGTTTTCAAACCATGATAACGCAAGGAGGGTTCTATACCAGTCTCAGAAGCACTCTCAGCAGGCCATTGGTTTTTCTGCCACAAATCCTAACCACAGAGTGGATACTCTGTCTCACCAACTACACTACCCTCAGAGGCCACTTTTTCATACTATGATATCTGATTGCCTTGGCCAACCAGGAAACCCAGTGAGCCATAACAGAGTTATGCCCAAGCCTGAATACTTTAATGGCCAAAATGCAATTGTTGCGGTCAATGTGCACCTAGGATATAACCAAGAAGATTCTATTGTGATGAACCGCGCATCTGTGCAACGTGGTATGTTCAAATCTGAGCACATACGGAGCTACAAGGCTGAAGTTGAAAACAAGTTCTCATTGGGAAAAAGGAGGAAGCCAGATGACTGTATAAATTTTGGCAAAATACAGAGTAAATTTGGAAGTGTTGACAGTCTTGATGATGATGGATTCCCCTGTATAGGTGCATATCTGCAGAGTGGTGATACTATTATTGCGAGGTGCAGTGAATCTGGTACTGATCATAGCATCAAACTGACGCATACTGAAAGAGGAAAGGTTCAGAAGGTTGTACTTTCCTCTAATGAAGAGGGAAAGAATTTTGGTGTTGTATCCTTAAGACAGGTTCGTTCTCCTTGCCTTGGAGACAAGTTCTCAAGCATGCATGGGCAGAAGGGTGTCCTAGGCTTTTTGGAGTCTGAAGAGAACTTCCCCTTCACAATACAGGGGATAGTTCCAGATATTGTAATTAATCCACATGCATTTCCTTCACGACAAGCTCCTGGTCAACTCCTAGAGGCTGCTTTAGGGAAGGGGATTGCCTGTGGTGGTCAACTCCTAGAGACAAGCTCCTGGTCAACTCCTAGAGACAAGCTCCTATATGCAACTCCTTTCTCTACTCCCTCTGTTGATGACATCACAGATCAGCTTCATAGAGCTGGATTTCAAAGATGGGGAAATGAGAGAGTATACAGTGGTCAAACTGGTCAAATGGTTCGCCAACTGATATTTATGGGCCCAACATTCTATCAACGTCTCATTCACATGTCTGAAGACAAAGTGAAGTTCAGGAACACTGGGCCAGTTCACCCTCTCACCCGGCAGCCAGTGGCAGACAGGAAGAGATTTGGTGGGGTAAAGTTTGGTGAGATGGAGCGCGACTGCCTTATAGCCCATGGAGCATCAGCAAACTTACATGAACGCCTTTTCACTCTTAGTGACTCCTCCCAAATGCATGCTTGCCTGAAATGCAAAAATGCGGCTAATGTGATAGATGGAACAGTTGATGGCAGTAGAATCAGGGGTCCATATTGCCGGGTCTCAGAGGGAACTGTTCAGGAGTATAATGCTATTATGACACTCAGGGATGAATGGGGCATCAACCCTCCAAGTTGGGTTGGTTCAGATCCTTGTGGCGACTGGGAAGGTATTACTTGCACCAATTCACAGATCACTGCTATGAATCTGGCTTTCAATGAGAACCTGAAGGGACAACTTCCAGCATCAATTGGAAACTTGACCAAACTAAACACCGTAATCCTGGATGGTTGCAGATTCTCTGGTCCTATTCCAGCTACAATAGGATCTTTAAAGCAACTTTCTTATCTATCTCTGAAAAACAATAGATTTAGTGGTCCAATTCCACCTTCCATTGGCAATCTAGCCAATCTTGTCTTTCTGGACCTATCGGACAACAGGCTTGGGGATTCCATTCCAGTCTCTAATGGGACTGCACCTGGTCTTGATATGTTAAATAAAACAAAGCACTTTCATTTTTCAAATAACCAGCTCTCGGGCAGCATTCCACCTCAACTGTTCAGCTCAAATATGCCTCTGATACATTTGATTTTTGACAGAAATAATTTTACTGGCAGCATCCCTTCCACACTTGGACTTGTGAAAACTCTGGAGGCAGTACGCCTTGATAGAAATTCATTAAGTGGGTCTGTACCTACGAGCCTCAGAAATCTTACAAATGTGTCTGAACTGCACCTGTCCAACAATAAGCTGACTGGCCCGGTGCCCAACCTTACTCTCATGGATTCCCTCAACTATGTGGATATGAGCAATAATACTTTTGATGCGTCAGATATTCCTAATTGGTTTTCAACCTTGAAGTTCTTGACAACATTAATGATGGAAAACACAGGACTTCAGGGACAAGTCCCTCAAGCCCTTTTCAGCCTTCAAAATTTGGAGACAGTGATACTGAAGAACAACAGTCTCAATGGTACATTGGATATTGGAACAAATTATGGCGACCAATTGAACCTAATTGATCTGCAGAAGAACAACATTGCTAATCTTGCACCAATTGATGAGGGATCCAACTACACATTGATTCTAGTTGACAATCCAATTTGTGAGGGGAGTCAGAATAACATGGCAAAGAACTACTGCAATGTTTCTCCTCCTAATTCCTCAATTTCAACTACACCAAGTAATTGTGCTGACTGTAGTTCCGGCCAGGTTGCTTCCCCCAACTGTAAATGTGCATCTCCCTATACACGGACTCTAGTCTTCTTATTTGTTTCTTTCTCAAACTTGGATGACGTCAATTATTACAAAGCTCTCTCAGACAATCTCACAAGATCTTTTCAGTCCAAAAACCTTCCTGTGGATTCAGTTTCACTGAGTCATCCGAGCTGGAGCTCATCTTATCAACTTGAAATTATCATAGAAATTTTCCCATCCGGTGATCAAGTTAGCTTCAACCAAACAGAAAGTTCAGCGATTACATCTGTTATTAGCAACCAGACTCTAGAGGGACGTCCAAATTCTTTTGGACCCTACTCTTTTCTTGTTCTTTACACCAACTCTGGAGGATCAAACAAGGCCGTGATAATTGGAACGGCAATTGGTGGTTCTGTGCTCCTTTCATTATTAGTCCTTGTAGGGCTTTATGCTCTCCACCAGAAGAGAAAAGCAGATGAGTCATTGAGTGGATCGATCCTTTTGCAGACAGGGATTTCAAATAGCAGTGCTAGTGGTCCTCAACTAAAAGGAGCAAGATTGTTCTCATTTGAAGAGCTCAAGAAATGCACCGATGGTTTTTCGGAAGCAAATGATATTGGATCTGGGGGTTATGGCCAGGTGTATAAGGGAATTCTTCCAACAGGCCAAATGGCTGCCATTAAAAGAGCTAAACGGGAATCTCTGCAGGGAGGAGTTGAGTTCAAAGCCGAGGTTGAGCTCCTATCACGAGTACATCATAAGAATCTTGTTGGCCTTGTAGGTTTTTGTTTTGAGCAAGGGGAACAAATATTGGTATATGAGTATGTTCCAAATGGTGATTTGAGGGACAGCCTTTCAGGTATTCCATGCACTTGCACTTTACAAAGCTTGTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000228 GO:0000418 GO:0000419 GO:0000428 GO:0000785 GO:0000790 GO:0000792 GO:0002682 GO:0003674 GO:0003824 GO:0003899 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005720 GO:0005730 GO:0005736 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009605 GO:0009607 GO:0009620 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016020 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019222 GO:0019438 GO:0030422 GO:0030880 GO:0031047 GO:0031050 GO:0031974 GO:0031981 GO:0032259 GO:0032774 GO:0032991 GO:0034062 GO:0034641 GO:0034654 GO:0035194 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043331 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044451 GO:0044452 GO:0044454 GO:0044464 GO:0044728 GO:0046483 GO:0048519 GO:0048583 GO:0050776 GO:0050789 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0055029 GO:0060255 GO:0061695 GO:0065007 GO:0070013 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0097747 GO:0098542 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1901698 GO:1901699 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko01100 ko03020 ko05016 ko05169 map00230 map00240 map01100 map03020 map05016 map05169
Pfam Domains
Protein Families
RNA_pol_Rpb2_1 RNA_pol_Rpb2_2 RNA_pol_Rpb2_3 RNA_pol_Rpb2_4 RNA_pol_Rpb2_5 RNA_pol_Rpb2_6 RNA_pol_Rpb2_7

Protein Analysis

1492

Amino Acids

164.63

Weight (kDa)

8.25

Isoelectric Point (pI)

37.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNA_pol_Rpb2_3 PF04565 143 - 202 3.8e-17 RNA polymerase Rpb2, domain 3
RNA_pol_Rpb2_4 PF04566 237 - 298 5.7e-21 RNA polymerase Rpb2, domain 4
RNA_pol_Rpb2_5 PF04567 313 - 358 2.7e-07 RNA polymerase Rpb2, domain 5
RNA_pol_Rpb2_6 PF00562 367 - 751 2e-103 RNA polymerase Rpb2, domain 6
RNA_pol_Rpb2_7 PF04560 753 - 814 1.1e-14 RNA polymerase Rpb2, domain 7
LRR_14 PF23598 873 - 938 8.5e-06 Leucine-rich repeat region
Pkinase PF00069 1395 - 1480 7e-12 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 1398 - 1480 2.9e-13 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 293
AatII GACGTC 3 cut(s) 1017, 3684, 3901
AccB1I GGYRCC 1 cut(s) 3148
AccB7I CCANNNNNTGG 1 cut(s) 1210
AccI GTMKAC 1 cut(s) 2097
AccII CGCG 3 cut(s) 17, 1413, 2286
AciI CCGC 5 cut(s) 15, 515, 1361, 1411, 2387
AclWI GGATC 9 cut(s) 951, 2528, 2719, 3447, 3460, 3961, 4072, 4081, 4210
AcoI YGGCCR 4 cut(s) 1282, 1342, 3592, 4216
AcuI CTGAAG 9 cut(s) 121, 242, 1002, 1488, 1848, 2199, 2633, 3266, 3359
AcyI GRCGYC 3 cut(s) 1014, 3681, 3898
AdeI CACNNNGTG 2 cut(s) 1064, 1210
AfiI CCNNNNNNNGG 8 cut(s) 75, 1210, 1589, 1769, 2780, 2920, 3636, 3745
AflII CTTAAG 2 cut(s) 1093, 1744
AflIII ACRYGT 3 cut(s) 229, 933, 2172
AjuI GAANNNNNNNTTGG 2 cut(s) 2758, 2790
Alw21I GWGCWC 7 cut(s) 972, 1374, 1449, 3782, 4002, 4161, 4323
Alw44I GTGCAC 1 cut(s) 1370
AlwI GGATC 9 cut(s) 951, 2528, 2719, 3447, 3460, 3961, 4072, 4081, 4210
AlwNI CAGNNNCTG 1 cut(s) 835
Ama87I CYCGRG 2 cut(s) 27, 2920
ApaI GGGCCC 1 cut(s) 2147
ApaLI GTGCAC 1 cut(s) 1370
ApeKI GCWGC 6 cut(s) 829, 1928, 2230, 2925, 3000, 4256
AseI ATTAAT 2 cut(s) 1878, 3260
AspA2I CCTAGG 2 cut(s) 1375, 1810
AspLEI GCGC 3 cut(s) 252, 1415, 2286
AsuC2I CCSGG 3 cut(s) 2227, 2444, 3146
AvaI CYCGRG 2 cut(s) 27, 2920
AvaII GGWCC 7 cut(s) 1072, 2432, 2690, 2762, 2809, 3914, 4118
AvrII CCTAGG 2 cut(s) 1375, 1810
BaeGI GKGCMC 3 cut(s) 1374, 2147, 3153
BalI TGGCCA 3 cut(s) 1284, 1344, 4218
BamHI GGATCC 1 cut(s) 3452
BanI GGYRCC 1 cut(s) 3148
BanII GRGCYC 6 cut(s) 630, 972, 2147, 3782, 4161, 4323
BauI CACGAG 1 cut(s) 4329
BbsI GAAGAC 3 cut(s) 470, 2187, 3640
Bbv12I GWGCWC 7 cut(s) 972, 1374, 1449, 3782, 4002, 4161, 4323
BbvI GCAGC 6 cut(s) 841, 1915, 2242, 2937, 3012, 4243
BceAI ACGGC 2 cut(s) 3950, 3996
BcgI CGANNNNNNTGC 4 cut(s) 77, 111, 4066, 4100
BciVI GTATCC 2 cut(s) 1207, 1750
BclI TGATCA 2 cut(s) 1648, 3826
BcnI CCSGG 3 cut(s) 2227, 2444, 3146
BfmI CTRYAG 5 cut(s) 324, 1601, 3410, 3583, 4286
BfrI CTTAAG 2 cut(s) 1093, 1744
BfuI GTATCC 2 cut(s) 1207, 1750
BglII AGATCT 1 cut(s) 3719
BisI GCNGC 7 cut(s) 830, 1929, 2231, 2388, 2926, 3001, 4257
BlnI CCTAGG 2 cut(s) 1375, 1810
BlsI GCNGC 7 cut(s) 831, 1930, 2232, 2389, 2927, 3002, 4258
Bme18I GGWCC 7 cut(s) 1072, 2432, 2690, 2762, 2809, 3914, 4118
BmeT110I CYCGRG 2 cut(s) 27, 2920
BmiI GGNNCC 6 cut(s) 627, 2145, 2433, 3150, 3454, 3916
BmrI ACTGGG 4 cut(s) 538, 1292, 2214, 2557
BmuI ACTGGG 4 cut(s) 538, 1292, 2214, 2557
BoxI GACNNNNGTC 1 cut(s) 3644
BpiI GAAGAC 3 cut(s) 470, 2187, 3640
BplI GAGNNNNNCTC 4 cut(s) 1961, 1993, 1986, 2018
BpmI CTGGAG 5 cut(s) 331, 384, 3056, 3796, 3969
BpuEI CTTGAG 3 cut(s) 1768, 3282, 4145
BpuMI CCSGG 3 cut(s) 2227, 2444, 3146
Bsa29I ATCGAT 1 cut(s) 4076
BsaAI YACGTR 1 cut(s) 232
BsaHI GRCGYC 3 cut(s) 1014, 3681, 3898
BsaI GGTCTC 2 cut(s) 267, 2452
BsaJI CCNNGG 7 cut(s) 26, 1279, 1375, 1768, 1810, 2302, 4311
BsaWI WCCGGW 1 cut(s) 3821
BsaXI ACNNNNNCTCC 4 cut(s) 386, 416, 1025, 1055
Bsc4I CCNNNNNNNGG 8 cut(s) 75, 1210, 1589, 1769, 2780, 2920, 3636, 3745
Bse3DI GCAATG 3 cut(s) 961, 3423, 3535
BseCI ATCGAT 1 cut(s) 4076
BseDI CCNNGG 7 cut(s) 26, 1279, 1375, 1768, 1810, 2302, 4311
BseLI CCNNNNNNNGG 8 cut(s) 75, 1210, 1589, 1769, 2780, 2920, 3636, 3745
BseMI GCAATG 3 cut(s) 961, 3423, 3535
BseRI GAGGAG 5 cut(s) 15, 677, 2341, 3528, 4308
BseSI GKGCMC 3 cut(s) 1374, 2147, 3153
BseXI GCAGC 6 cut(s) 841, 1915, 2242, 2937, 3012, 4243
BsgI GTGCAG 4 cut(s) 585, 1651, 2844, 3101
Bsh1236I CGCG 3 cut(s) 17, 1413, 2286
BshNI GGYRCC 1 cut(s) 3148
BshVI ATCGAT 1 cut(s) 4076
BsiHKAI GWGCWC 7 cut(s) 972, 1374, 1449, 3782, 4002, 4161, 4323
BsiHKCI CYCGRG 2 cut(s) 27, 2920
BsiSI CCGG 5 cut(s) 2227, 2443, 3146, 3591, 3822
BslFI GGGAC 7 cut(s) 1025, 2631, 2869, 3278, 3300, 3909, 4442
BslI CCNNNNNNNGG 8 cut(s) 75, 1210, 1589, 1769, 2780, 2920, 3636, 3745
BsmBI CGTCTC 2 cut(s) 775, 2168
BsmFI GGGAC 7 cut(s) 1025, 2631, 2869, 3278, 3300, 3909, 4442
BsmI GAATGC 3 cut(s) 50, 353, 2928
Bso31I GGTCTC 2 cut(s) 267, 2452
BsoBI CYCGRG 2 cut(s) 27, 2920
Bsp120I GGGCCC 1 cut(s) 2143
Bsp1407I TGTACA 1 cut(s) 1001
Bsp19I CCATGG 1 cut(s) 2302
BspACI CCGC 5 cut(s) 15, 515, 1361, 1411, 2387
BspDI ATCGAT 1 cut(s) 4076
BspFNI CGCG 3 cut(s) 17, 1413, 2286
BspHI TCATGA 1 cut(s) 762
BspLI GGNNCC 6 cut(s) 627, 2145, 2433, 3150, 3454, 3916
BspMAI CTGCAG 3 cut(s) 1605, 3414, 4290
BspPI GGATC 9 cut(s) 951, 2528, 2719, 3447, 3460, 3961, 4072, 4081, 4210
BspQI GCTCTTC 1 cut(s) 4149
BspT107I GGYRCC 1 cut(s) 3148
BspTI CTTAAG 2 cut(s) 1093, 1744
BspTNI GGTCTC 2 cut(s) 267, 2452
BsrDI GCAATG 3 cut(s) 961, 3423, 3535
BsrGI TGTACA 1 cut(s) 1001
BssECI CCNNGG 7 cut(s) 26, 1279, 1375, 1768, 1810, 2302, 4311
BssNAI GTATAC 1 cut(s) 2098
BssNI GRCGYC 3 cut(s) 1014, 3681, 3898
BssSI CACGAG 1 cut(s) 4329
BssT1I CCWWGG 5 cut(s) 1279, 1375, 1768, 1810, 2302
Bst1107I GTATAC 1 cut(s) 2098
Bst2BI CACGAG 1 cut(s) 4329
Bst6I CTCTTC 6 cut(s) 984, 1710, 1824, 2243, 4040, 4149
BstACI GRCGYC 3 cut(s) 1014, 3681, 3898
BstAFI CTTAAG 2 cut(s) 1093, 1744
BstAPI GCANNNNNTGC 1 cut(s) 1358
BstAUI TGTACA 1 cut(s) 1001
BstBAI YACGTR 1 cut(s) 232
BstC8I GCNNGC 4 cut(s) 1173, 1790, 2364, 2368
BstDSI CCRYGG 1 cut(s) 2302
BstEII GGTNACC 1 cut(s) 284
BstFNI CGCG 3 cut(s) 17, 1413, 2286
BstHHI GCGC 3 cut(s) 252, 1415, 2286
BstMWI GCNNNNNNNGC 8 cut(s) 211, 599, 634, 1168, 1358, 2306, 2376, 3434
BstNSI RCATGY 6 cut(s) 58, 242, 1792, 1889, 2176, 2366
BstPAI GACNNNNGTC 1 cut(s) 3644
BstPI GGTNACC 1 cut(s) 284
BstSFI CTRYAG 5 cut(s) 324, 1601, 3410, 3583, 4286
BstSLI GKGCMC 3 cut(s) 1374, 2147, 3153
BstUI CGCG 3 cut(s) 17, 1413, 2286
BstV1I GCAGC 6 cut(s) 841, 1915, 2242, 2937, 3012, 4243
BstV2I GAAGAC 3 cut(s) 470, 2187, 3640
BstX2I RGATCY 6 cut(s) 943, 2533, 2711, 3452, 3719, 4202
BstXI CCANNNNNNTGG 1 cut(s) 604
BstYI RGATCY 6 cut(s) 943, 2533, 2711, 3452, 3719, 4202
BstZ17I GTATAC 1 cut(s) 2098
Bsu15I ATCGAT 1 cut(s) 4076
BsuI GTATCC 2 cut(s) 1207, 1750
BsuTUI ATCGAT 1 cut(s) 4076
BtgI CCRYGG 1 cut(s) 2302
BtsI GCAGTG 5 cut(s) 437, 597, 1639, 2580, 4114
Cac8I GCNNGC 4 cut(s) 1173, 1790, 2364, 2368
CaiI CAGNNNCTG 1 cut(s) 835
CciI TCATGA 1 cut(s) 762
CfoI GCGC 3 cut(s) 252, 1415, 2286
ClaI ATCGAT 1 cut(s) 4076
CseI GACGC 3 cut(s) 792, 1675, 3201
CsiI ACCWGGT 1 cut(s) 2863
DraI TTTAAA 1 cut(s) 2718
DraIII CACNNNGTG 2 cut(s) 1064, 1210
DrdI GACNNNNNNGTC 1 cut(s) 293
DseDI GACNNNNNNGTC 1 cut(s) 293
EaeI YGGCCR 4 cut(s) 1282, 1342, 3592, 4216
Eam1104I CTCTTC 6 cut(s) 984, 1710, 1824, 2243, 4040, 4149
EarI CTCTTC 6 cut(s) 984, 1710, 1824, 2243, 4040, 4149
Ecl136II GAGCTC 4 cut(s) 970, 3780, 4159, 4321
Eco130I CCWWGG 5 cut(s) 1279, 1375, 1768, 1810, 2302
Eco24I GRGCYC 6 cut(s) 630, 972, 2147, 3782, 4161, 4323
Eco31I GGTCTC 2 cut(s) 267, 2452
Eco32I GATATC 2 cut(s) 512, 1269
Eco47I GGWCC 7 cut(s) 1072, 2432, 2690, 2762, 2809, 3914, 4118
Eco53kI GAGCTC 4 cut(s) 970, 3780, 4159, 4321
Eco57I CTGAAG 9 cut(s) 121, 242, 1002, 1488, 1848, 2199, 2633, 3266, 3359
Eco88I CYCGRG 2 cut(s) 27, 2920
Eco91I GGTNACC 1 cut(s) 284
EcoICRI GAGCTC 4 cut(s) 970, 3780, 4159, 4321
EcoO65I GGTNACC 1 cut(s) 284
EcoRI GAATTC 1 cut(s) 4233
EcoRV GATATC 2 cut(s) 512, 1269
EcoT14I CCWWGG 5 cut(s) 1279, 1375, 1768, 1810, 2302
EcoT22I ATGCAT 4 cut(s) 50, 1794, 1891, 2364
EcoT38I GRGCYC 6 cut(s) 630, 972, 2147, 3782, 4161, 4323
ErhI CCWWGG 5 cut(s) 1279, 1375, 1768, 1810, 2302
Esp3I CGTCTC 2 cut(s) 775, 2168
FaqI GGGAC 7 cut(s) 1025, 2631, 2869, 3278, 3300, 3909, 4442
FbaI TGATCA 2 cut(s) 1648, 3826
FblI GTMKAC 1 cut(s) 2097
Fnu4HI GCNGC 7 cut(s) 830, 1929, 2231, 2388, 2926, 3001, 4257
FriOI GRGCYC 6 cut(s) 630, 972, 2147, 3782, 4161, 4323
Fsp4HI GCNGC 7 cut(s) 830, 1929, 2231, 2388, 2926, 3001, 4257
GlaI GCGC 3 cut(s) 251, 1414, 2285
GluI GCNGC 7 cut(s) 830, 1929, 2231, 2388, 2926, 3001, 4257
GsuI CTGGAG 5 cut(s) 331, 384, 3056, 3796, 3969
HapII CCGG 5 cut(s) 2227, 2443, 3146, 3591, 3822
HgaI GACGC 3 cut(s) 792, 1675, 3201
HhaI GCGC 3 cut(s) 252, 1415, 2286
Hin1I GRCGYC 3 cut(s) 1014, 3681, 3898
Hin6I GCGC 3 cut(s) 250, 1413, 2284
HinP1I GCGC 3 cut(s) 250, 1413, 2284
HincII GTYRAC 5 cut(s) 1558, 1916, 1961, 1986, 3478
HindII GTYRAC 5 cut(s) 1558, 1916, 1961, 1986, 3478
HindIII AAGCTT 1 cut(s) 4468
HpaII CCGG 5 cut(s) 2227, 2443, 3146, 3591, 3822
HpyCH4IV ACGT 8 cut(s) 231, 874, 935, 1014, 1426, 2161, 3681, 3898
HpyF10VI GCNNNNNNNGC 8 cut(s) 211, 599, 634, 1168, 1358, 2306, 2376, 3434
HpySE526I ACGT 8 cut(s) 231, 874, 935, 1014, 1426, 2161, 3681, 3898
Hsp92I GRCGYC 3 cut(s) 1014, 3681, 3898
HspAI GCGC 3 cut(s) 250, 1413, 2284
Ksp22I TGATCA 2 cut(s) 1648, 3826
LguI GCTCTTC 1 cut(s) 4149
Lsp1109I GCAGC 6 cut(s) 841, 1915, 2242, 2937, 3012, 4243
MabI ACCWGGT 1 cut(s) 2863
MaeII ACGT 8 cut(s) 231, 874, 935, 1014, 1426, 2161, 3681, 3898
MaeIII GTNAC 4 cut(s) 155, 284, 331, 2345
MfeI CAATTG 5 cut(s) 1353, 2634, 3392, 3441, 3984
MflI RGATCY 6 cut(s) 943, 2533, 2711, 3452, 3719, 4202
MlsI TGGCCA 3 cut(s) 1284, 1344, 4218
MluNI TGGCCA 3 cut(s) 1284, 1344, 4218
MlyI GAGTC 7 cut(s) 1832, 2342, 3508, 3634, 3772, 3880, 4070
MmeI TCCRAC 4 cut(s) 478, 3150, 3480, 4265
Mox20I TGGCCA 3 cut(s) 1284, 1344, 4218
Mph1103I ATGCAT 4 cut(s) 50, 1794, 1891, 2364
MscI TGGCCA 3 cut(s) 1284, 1344, 4218
MslI CAYNNNNRTG 3 cut(s) 53, 2171, 2585
Msp20I TGGCCA 3 cut(s) 1284, 1344, 4218
MspCI CTTAAG 2 cut(s) 1093, 1744
MspI CCGG 5 cut(s) 2227, 2443, 3146, 3591, 3822
MunI CAATTG 5 cut(s) 1353, 2634, 3392, 3441, 3984
Mva1269I GAATGC 3 cut(s) 50, 353, 2928
MvnI CGCG 3 cut(s) 17, 1413, 2286
MwoI GCNNNNNNNGC 8 cut(s) 211, 599, 634, 1168, 1358, 2306, 2376, 3434
NciI CCSGG 3 cut(s) 2227, 2444, 3146
NcoI CCATGG 1 cut(s) 2302
NlaIV GGNNCC 6 cut(s) 627, 2145, 2433, 3150, 3454, 3916
NmeAIII GCCGAG 1 cut(s) 4336
NmuCI GTSAC 3 cut(s) 155, 284, 2345
NsiI ATGCAT 4 cut(s) 50, 1794, 1891, 2364
NspI RCATGY 6 cut(s) 58, 242, 1792, 1889, 2176, 2366
PaeI GCATGC 2 cut(s) 1792, 2366
PagI TCATGA 1 cut(s) 762
PciI ACATGT 1 cut(s) 2172
PciSI GCTCTTC 1 cut(s) 4149
PctI GAATGC 3 cut(s) 50, 353, 2928
PflMI CCANNNNNTGG 1 cut(s) 1210
PfoI TCCNGGA 3 cut(s) 834, 939, 2667
PkrI GCNGC 7 cut(s) 831, 1930, 2232, 2389, 2927, 3002, 4258
PleI GAGTC 7 cut(s) 1831, 2342, 3507, 3634, 3771, 3880, 4069
PpsI GAGTC 7 cut(s) 1831, 2342, 3507, 3634, 3771, 3880, 4069
Ppu21I YACGTR 1 cut(s) 232
PscI ACATGT 1 cut(s) 2172
PshAI GACNNNNGTC 1 cut(s) 3644
PshBI ATTAAT 2 cut(s) 1878, 3260
Psp124BI GAGCTC 4 cut(s) 972, 3782, 4161, 4323
PspEI GGTNACC 1 cut(s) 284
PspN4I GGNNCC 6 cut(s) 627, 2145, 2433, 3150, 3454, 3916
PspOMI GGGCCC 1 cut(s) 2143
PstI CTGCAG 3 cut(s) 1605, 3414, 4290
PstNI CAGNNNCTG 1 cut(s) 835
PsuI RGATCY 6 cut(s) 943, 2533, 2711, 3452, 3719, 4202
RseI CAYNNNNRTG 3 cut(s) 53, 2171, 2585
SacI GAGCTC 4 cut(s) 972, 3782, 4161, 4323
SapI GCTCTTC 1 cut(s) 4149
SatI GCNGC 7 cut(s) 830, 1929, 2231, 2388, 2926, 3001, 4257
SchI GAGTC 7 cut(s) 1832, 2342, 3508, 3634, 3772, 3880, 4070
SexAI ACCWGGT 1 cut(s) 2863
SfcI CTRYAG 5 cut(s) 324, 1601, 3410, 3583, 4286
SinI GGWCC 7 cut(s) 1072, 2432, 2690, 2762, 2809, 3914, 4118
SmiMI CAYNNNNRTG 3 cut(s) 53, 2171, 2585
SmlI CTYRAG 5 cut(s) 1093, 1744, 1783, 3297, 4160
SmoI CTYRAG 5 cut(s) 1093, 1744, 1783, 3297, 4160
SphI GCATGC 2 cut(s) 1792, 2366
SsiI CCGC 5 cut(s) 15, 515, 1361, 1411, 2387
SspI AATATT 1 cut(s) 4392
SstI GAGCTC 4 cut(s) 972, 3782, 4161, 4323
StyI CCWWGG 5 cut(s) 1279, 1375, 1768, 1810, 2302
TaiI ACGT 8 cut(s) 234, 877, 938, 1017, 1429, 2164, 3684, 3901
TaqI TCGA 2 cut(s) 966, 4076
TatI WGTACW 5 cut(s) 611, 1001, 1053, 1699, 4333
TauI GCSGC 1 cut(s) 2390
TseFI GTSAC 3 cut(s) 155, 284, 2345
TseI GCWGC 6 cut(s) 829, 1928, 2230, 2925, 3000, 4256
Tsp45I GTSAC 3 cut(s) 155, 284, 2345
TspGWI ACGGA 3 cut(s) 1101, 1468, 3652
Van91I CCANNNNNTGG 1 cut(s) 1210
Vha464I CTTAAG 2 cut(s) 1093, 1744
VneI GTGCAC 1 cut(s) 1370
VpaK11BI GGWCC 7 cut(s) 1072, 2432, 2690, 2762, 2809, 3914, 4118
VspI ATTAAT 2 cut(s) 1878, 3260
XbaI TCTAGA 3 cut(s) 208, 316, 3889
XceI RCATGY 6 cut(s) 58, 242, 1792, 1889, 2176, 2366
XcmI CCANNNNNNNNNTGG 1 cut(s) 947
XmaJI CCTAGG 2 cut(s) 1375, 1810
XmiI GTMKAC 1 cut(s) 2097
ZraI GACGTC 3 cut(s) 1015, 3682, 3899
Zsp2I ATGCAT 4 cut(s) 50, 1794, 1891, 2364
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.