RchiOBHm_Chr5g0042281

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0002206
Physical Location & Seq
Reverse (-)
37051314 .. 37057717
6404 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ32069

Sequence Viewer

Length: 2871 bp
ATGGTGAAGTCTGACTTGTGTTGGATGAAAGACGAGAAAGCTGACTGTGAATTTGACCATGGAGGTTATTTTATAATCAAGGGTGCAGAGAAGAGAGTTTTGTCAGTGTACTTTAAGTCAGCAGAAATCCCTCTCTGGATTTGGTTTTTTGCCCTTGGGGTTTCTTCGGACAATGAAGTTACAGATCTGATTGATTATGGCAAAGAAGATGCAAGCATTTCAAGCATTCTAGTTGCCTCAATTCGTGATGCTGACCAACGAAAAGATAACTTCCGGAGAGGGAAGAATGCTCTCAGATATGTTGAAGAGATGATAAAGAATACCGCGTTTCCTCCTCGGGAAAGCATTGAAGAATGCATCAGCATGTATCTGTTCCCCAATCTCAGGGGCTTGAAGCAAAAGGCTCGGTTTCTTGGCTATATGGTGAAGTCTCTTTTACAGACCTTCAGAGGCCACAGGAAATGTGACAACAGAGAAGAATTGAAGAACAAGAGGTTTCACTTGGCTGGTGAGCTTCTAGAGCGAGAGCTGAAGGCACACGTAGGACATGCAGGAAGGCGCATGGCAAAGGCTTTGCAGAGAGACCTTTGTGGTGACCGTGTTGTCTGCCTGGTTGAGCAATATCTAGATGCTACAGTAGTAACCAATGGCATCTCCAGAGCATTCTCAACTGGAGCGTGGTGTCATCCTTTCAAGAGGATGGAGAGGATGTCTGGTGTGGTGGTAAATGTTGGGAAAACAAATCCACTGCAAACAATGGTAGATATGAGGAAGACACGCCTCCAAGTTCAATACACGGGGAAGGTCGGAGATGCCAGATATCCGCATCCTTCTCACTGGGGTAGGGTTTGCTTTATCTCAACCCCAGATGGTGAGAATTGTGGGCTTGTGAAAAATTTGGCTACCACTGCACTGGTAAGTACAAACATATTGGAGCCCATATTGCCCGACTTTTTCTCTAGTGGCATGGAGGAGCTGGTAGATGATACTTCAACATCACTTCATGGGAAGAATAAAGTTTTTCTCAATGGGGATTGGGTTGGTGTTTGTGAAGATTCCCATGATTTTGTCATGAAACTAAGGACATTGAGACGCAGGAAAAAGTTGCTACATCAGGTGGAAATCAAAAGAGATGAGCAGCTCCTGGAAGTACGCATATATTCTGATGCTGGAAGGATTCTACGTCCTTTCCTGGTAGTTAAGAATTTGAATAAGATTAAAGAATCAAAAGGGAAAAAACACACGTTCCAGGATCTTCTTGGCAATGGAATAATCGAGCTCATTGGAGCTGAAGAAGAAGAGGATTGCTGTACAGCATGGGACGTCAAGCATCTATTTATGGAGGGGAAGTCAGCAGTCAAGTACACACACTGTGAACTGGACCTATCATTTTTGTTAGGCTTAAGTTGTGGTATCATTCCGTTTTCAAACCATGATAACGCAAGGAGGGTTCTATACCAGTCTCAGAAGCACTCTCAGCAGGCCATTGGTTTTTCTGCCACAAATCCTAACCACAGAGTGGATACTCTGTCTCACCAACTACACTACCCTCAGAGGCCACTTTTTCATACTATGATATCTGATTGCCTTGGCCAACCAGGAAACCCAGTGAGCCATAACAGAGTTATGCCCAAGCCTGAATACTTTAATGGCCAAAATGCAATTGTTGCGGTCAATGTGCACCTAGGATATAACCAAGAAGATTCTATTGTGATGAACCGCGCATCTGTGCAACGTGGTATGTTCAAATCTGAGCACATACGGAGCTACAAGGCTGAAGTTGAAAACAAGTTCTCATTGGGAAAAAGGAGGAAGCCAGATGACTGTATAAATTTTGGCAAAATACAGAGTAAATTTGGAAGTGTTGACAGTCTTGATGATGATGGATTCCCCTGTATAGGTGCATATCTGCAGAGTGGTGATACTATTATTGCGAGGTGCAGTGAATCTGGTACTGATCATAGCATCAAACTGACGCATACTGAAAGAGGAAAGGTTCAGAAGGTTGTACTTTCCTCTAATGAAGAGGGAAAGAATTTTGGTGTTGTATCCTTAAGACAGGTTCGTTCTCCTTGCCTTGGAGACAAGTTCTCAAGCATGCATGGGCAGAAGGGTGTCCTAGGCTTTTTGGAGTCTGAAGAGAACTTCCCCTTCACAATACAGGGGATAGTTCCAGATATTGTAATTAATCCACATGCATTTCCTTCACGACAAGCTCCTGGTCAACTCCTAGAGGCTGCTTTAGGGAAGGGGATTGCCTGTGGTGGTCAACTCCTAGAGACAAGCTCCTGGTCAACTCCTAGAGACAAGCTCCTATATGCAACTCCTTTCTCTACTCCCTCTGTTGATGACATCACAGATCAGCTTCATAGAGCTGGATTTCAAAGATGGGGAAATGAGAGAGTATACAGTGGTCAAACTGGTCAAATGGTTCGCCAACTGATATTTATGGGCCCAACATTCTATCAACGTCTCATTCACATGTCTGAAGACAAAGTGAAGTTCAGGAACACTGGGCCAGTTCACCCTCTCACCCGGCAGCCAGTGGCAGACAGGAAGAGATTTGGTGGGGTAAAGTTTGGTGAGATGGAGCGCGACTGCCTTATAGCCCATGGAGCATCAGCAAACTTACATGAACGCCTTTTCACTCTTAGTGACTCCTCCCAAATGCATGCTTGCCTGAAATGCAAAAATGCGGCTAATGTGATAGATGGAACAGTTGATGGCAGTAGAATCAGGGGTCCATATTGCCGGGTCTGTAAGTCTGTTGATGACATTGTCAGGTTGAATGTTCCATATGGGGCCAAGTTATTGTGCCAGGAACTTTTCTGCATGGGCATAAGTCTCAAGTTTGAGACCAGGCTCTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000228 GO:0000418 GO:0000419 GO:0000428 GO:0000785 GO:0000790 GO:0000792 GO:0002682 GO:0003674 GO:0003824 GO:0003899 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005720 GO:0005730 GO:0005736 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009605 GO:0009607 GO:0009620 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016020 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019222 GO:0019438 GO:0030422 GO:0030880 GO:0031047 GO:0031050 GO:0031974 GO:0031981 GO:0032259 GO:0032774 GO:0032991 GO:0034062 GO:0034641 GO:0034654 GO:0035194 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043331 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044451 GO:0044452 GO:0044454 GO:0044464 GO:0044728 GO:0046483 GO:0048519 GO:0048583 GO:0050776 GO:0050789 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0055029 GO:0060255 GO:0061695 GO:0065007 GO:0070013 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0097747 GO:0098542 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1901698 GO:1901699 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko01100 ko03020 ko05016 ko05169 map00230 map00240 map01100 map03020 map05016 map05169
Pfam Domains
Protein Families
RNA_pol_Rpb2_1 RNA_pol_Rpb2_2 RNA_pol_Rpb2_3 RNA_pol_Rpb2_4 RNA_pol_Rpb2_5 RNA_pol_Rpb2_6 RNA_pol_Rpb2_7

Protein Analysis

956

Amino Acids

107.76

Weight (kDa)

8.84

Isoelectric Point (pI)

37.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNA_pol_Rpb2_1 PF04563 1 - 199 2.3e-06 RNA polymerase beta subunit
RNA_pol_Rpb2_2 PF04561 28 - 165 9.2e-09 RNA polymerase Rpb2, domain 2
RNA_pol_Rpb2_3 PF04565 246 - 305 2.2e-17 RNA polymerase Rpb2, domain 3
RNA_pol_Rpb2_4 PF04566 340 - 401 3.3e-21 RNA polymerase Rpb2, domain 4
RNA_pol_Rpb2_5 PF04567 416 - 461 1.6e-07 RNA polymerase Rpb2, domain 5
RNA_pol_Rpb2_6 PF00562 470 - 854 8.2e-104 RNA polymerase Rpb2, domain 6
RNA_pol_Rpb2_7 PF04560 856 - 952 1.2e-24 RNA polymerase Rpb2, domain 7
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 74
AasI GACNNNNNNGTC 1 cut(s) 602
AatII GACGTC 1 cut(s) 1326
AccB7I CCANNNNNTGG 1 cut(s) 1519
AccI GTMKAC 1 cut(s) 2406
AccII CGCG 3 cut(s) 326, 1722, 2595
AccIII TCCGGA 1 cut(s) 273
AciI CCGC 5 cut(s) 324, 824, 1670, 1720, 2696
AclWI GGATC 1 cut(s) 1260
AcoI YGGCCR 2 cut(s) 1591, 1651
AcsI RAATTY 6 cut(s) 50, 895, 1204, 1831, 1853, 2035
AcuI CTGAAG 6 cut(s) 430, 551, 1311, 1797, 2157, 2508
AcyI GRCGYC 1 cut(s) 1323
AdeI CACNNNGTG 2 cut(s) 1373, 1519
AfaI GTAC 7 cut(s) 110, 922, 1152, 1312, 1364, 1954, 2010
AfiI CCNNNNNNNGG 4 cut(s) 384, 1519, 1898, 2078
AflII CTTAAG 2 cut(s) 1402, 2053
AflIII ACRYGT 3 cut(s) 538, 1242, 2481
AjnI CCWGG 9 cut(s) 609, 1143, 1191, 1248, 1597, 2218, 2288, 2817, 2858
Alw21I GWGCWC 3 cut(s) 1281, 1683, 1758
Alw44I GTGCAC 1 cut(s) 1679
AlwI GGATC 1 cut(s) 1260
AlwNI CAGNNNCTG 2 cut(s) 1144, 2865
Ama87I CYCGRG 1 cut(s) 336
Aor13HI TCCGGA 1 cut(s) 273
AoxI GGCC 8 cut(s) 451, 1482, 1556, 1591, 1651, 2452, 2516, 2802
ApaI GGGCCC 1 cut(s) 2456
ApaLI GTGCAC 1 cut(s) 1679
ApeKI GCWGC 3 cut(s) 1138, 2237, 2539
ApoI RAATTY 6 cut(s) 50, 895, 1204, 1831, 1853, 2035
ArsI GACNNNNNNTTYG 2 cut(s) 35, 67
AseI ATTAAT 1 cut(s) 2187
AspA2I CCTAGG 2 cut(s) 1684, 2119
AspLEI GCGC 3 cut(s) 561, 1724, 2595
AspS9I GGNCC 6 cut(s) 1381, 2452, 2453, 2516, 2741, 2802
AsuC2I CCSGG 2 cut(s) 2536, 2753
AvaI CYCGRG 1 cut(s) 336
AvaII GGWCC 2 cut(s) 1381, 2741
AvrII CCTAGG 2 cut(s) 1684, 2119
BaeGI GKGCMC 2 cut(s) 1683, 2456
BalI TGGCCA 2 cut(s) 1593, 1653
BanII GRGCYC 3 cut(s) 939, 1281, 2456
BbsI GAAGAC 2 cut(s) 779, 2496
Bbv12I GWGCWC 3 cut(s) 1281, 1683, 1758
BbvI GCAGC 3 cut(s) 1150, 2224, 2551
BccI CCATC 7 cut(s) 694, 863, 1877, 2382, 2581, 2705, 2717
BcgI CGANNNNNNTGC 2 cut(s) 386, 420
BciT130I CCWGG 9 cut(s) 611, 1145, 1193, 1250, 1599, 2220, 2290, 2819, 2860
BciVI GTATCC 2 cut(s) 1516, 2059
BclI TGATCA 1 cut(s) 1957
BcnI CCSGG 2 cut(s) 2536, 2753
BfaI CTAG 9 cut(s) 230, 518, 626, 960, 1685, 2120, 2231, 2276, 2301
BfmI CTRYAG 2 cut(s) 633, 1910
BfrI CTTAAG 2 cut(s) 1402, 2053
BfuI GTATCC 2 cut(s) 1516, 2059
BglII AGATCT 1 cut(s) 184
BisI GCNGC 4 cut(s) 1139, 2238, 2540, 2697
BlnI CCTAGG 2 cut(s) 1684, 2119
BlsI GCNGC 4 cut(s) 1140, 2239, 2541, 2698
Bme18I GGWCC 2 cut(s) 1381, 2741
BmeT110I CYCGRG 1 cut(s) 336
BmgT120I GGNCC 6 cut(s) 1381, 2452, 2453, 2516, 2741, 2802
BmiI GGNNCC 4 cut(s) 936, 2454, 2742, 2803
BmrI ACTGGG 3 cut(s) 847, 1601, 2523
BmuI ACTGGG 3 cut(s) 847, 1601, 2523
BpiI GAAGAC 2 cut(s) 779, 2496
BplI GAGNNNNNCTC 4 cut(s) 2270, 2302, 2295, 2327
BpmI CTGGAG 2 cut(s) 640, 693
BpuEI CTTGAG 2 cut(s) 2077, 2831
BpuMI CCSGG 2 cut(s) 2536, 2753
BsaAI YACGTR 1 cut(s) 541
BsaHI GRCGYC 1 cut(s) 1323
BsaI GGTCTC 2 cut(s) 576, 2849
BsaJI CCNNGG 8 cut(s) 58, 154, 335, 1588, 1684, 2077, 2119, 2611
BsaWI WCCGGW 1 cut(s) 273
BsaXI ACNNNNNCTCC 4 cut(s) 695, 725, 1334, 1364
Bsc4I CCNNNNNNNGG 4 cut(s) 384, 1519, 1898, 2078
Bse3DI GCAATG 1 cut(s) 1270
BseAI TCCGGA 1 cut(s) 273
BseBI CCWGG 9 cut(s) 611, 1145, 1193, 1250, 1599, 2220, 2290, 2819, 2860
BseDI CCNNGG 8 cut(s) 58, 154, 335, 1588, 1684, 2077, 2119, 2611
BseGI GGATG 5 cut(s) 30, 685, 705, 714, 826
BseLI CCNNNNNNNGG 4 cut(s) 384, 1519, 1898, 2078
BseMI GCAATG 1 cut(s) 1270
BseMII CTCAG 6 cut(s) 307, 397, 1478, 1490, 1565, 1743
BseRI GAGGAG 3 cut(s) 324, 986, 2650
BseSI GKGCMC 2 cut(s) 1683, 2456
BseXI GCAGC 3 cut(s) 1150, 2224, 2551
BsgI GTGCAG 3 cut(s) 105, 894, 1960
Bsh1236I CGCG 3 cut(s) 326, 1722, 2595
BshFI GGCC 8 cut(s) 453, 1484, 1558, 1593, 1653, 2454, 2518, 2804
BsiHKAI GWGCWC 3 cut(s) 1281, 1683, 1758
BsiHKCI CYCGRG 1 cut(s) 336
BsiSI CCGG 3 cut(s) 274, 2536, 2752
BslFI GGGAC 1 cut(s) 1334
BslI CCNNNNNNNGG 4 cut(s) 384, 1519, 1898, 2078
BsmBI CGTCTC 2 cut(s) 1084, 2477
BsmFI GGGAC 1 cut(s) 1334
BsmI GAATGC 4 cut(s) 225, 292, 359, 662
BsnI GGCC 8 cut(s) 453, 1484, 1558, 1593, 1653, 2454, 2518, 2804
Bso31I GGTCTC 2 cut(s) 576, 2849
BsoBI CYCGRG 1 cut(s) 336
Bsp120I GGGCCC 1 cut(s) 2452
Bsp1286I GDGCHC 5 cut(s) 939, 1281, 1683, 1758, 2456
Bsp13I TCCGGA 1 cut(s) 273
Bsp1407I TGTACA 1 cut(s) 1310
Bsp143I GATC 4 cut(s) 184, 1252, 1957, 2359
Bsp19I CCATGG 2 cut(s) 58, 2611
BspACI CCGC 5 cut(s) 324, 824, 1670, 1720, 2696
BspANI GGCC 8 cut(s) 453, 1484, 1558, 1593, 1653, 2454, 2518, 2804
BspCNI CTCAG 6 cut(s) 306, 396, 1477, 1489, 1564, 1744
BspEI TCCGGA 1 cut(s) 273
BspFNI CGCG 3 cut(s) 326, 1722, 2595
BspHI TCATGA 1 cut(s) 1071
BspLI GGNNCC 4 cut(s) 936, 2454, 2742, 2803
BspMAI CTGCAG 1 cut(s) 1914
BspPI GGATC 1 cut(s) 1260
BspTI CTTAAG 2 cut(s) 1402, 2053
BspTNI GGTCTC 2 cut(s) 576, 2849
BsrDI GCAATG 1 cut(s) 1270
BsrGI TGTACA 1 cut(s) 1310
BssECI CCNNGG 8 cut(s) 58, 154, 335, 1588, 1684, 2077, 2119, 2611
BssMI GATC 4 cut(s) 184, 1252, 1957, 2359
BssNAI GTATAC 1 cut(s) 2407
BssNI GRCGYC 1 cut(s) 1323
BssT1I CCWWGG 7 cut(s) 58, 154, 1588, 1684, 2077, 2119, 2611
Bst1107I GTATAC 1 cut(s) 2407
Bst2UI CCWGG 9 cut(s) 611, 1145, 1193, 1250, 1599, 2220, 2290, 2819, 2860
Bst4CI ACNGT 8 cut(s) 47, 599, 637, 1373, 1826, 1871, 2411, 2719
Bst6I CTCTTC 6 cut(s) 86, 300, 1293, 2019, 2133, 2552
BstACI GRCGYC 1 cut(s) 1323
BstAFI CTTAAG 2 cut(s) 1402, 2053
BstAPI GCANNNNNTGC 1 cut(s) 1667
BstAUI TGTACA 1 cut(s) 1310
BstBAI YACGTR 1 cut(s) 541
BstC8I GCNNGC 5 cut(s) 214, 1482, 2099, 2673, 2677
BstDEI CTNAG 8 cut(s) 293, 383, 1079, 1464, 1476, 1551, 1752, 2651
BstDSI CCRYGG 2 cut(s) 58, 2611
BstEII GGTNACC 1 cut(s) 593
BstF5I GGATG 5 cut(s) 30, 685, 705, 714, 826
BstFNI CGCG 3 cut(s) 326, 1722, 2595
BstHHI GCGC 3 cut(s) 561, 1724, 2595
BstKTI GATC 4 cut(s) 187, 1255, 1960, 2362
BstMBI GATC 4 cut(s) 184, 1252, 1957, 2359
BstMWI GCNNNNNNNGC 8 cut(s) 222, 520, 908, 943, 1477, 1667, 2615, 2685
BstNI CCWGG 9 cut(s) 611, 1145, 1193, 1250, 1599, 2220, 2290, 2819, 2860
BstNSI RCATGY 6 cut(s) 367, 551, 2101, 2198, 2485, 2675
BstPI GGTNACC 1 cut(s) 593
BstSFI CTRYAG 2 cut(s) 633, 1910
BstSLI GKGCMC 2 cut(s) 1683, 2456
BstUI CGCG 3 cut(s) 326, 1722, 2595
BstV1I GCAGC 3 cut(s) 1150, 2224, 2551
BstV2I GAAGAC 2 cut(s) 779, 2496
BstX2I RGATCY 2 cut(s) 184, 1252
BstXI CCANNNNNNTGG 1 cut(s) 913
BstYI RGATCY 2 cut(s) 184, 1252
BstZ17I GTATAC 1 cut(s) 2407
BsuI GTATCC 2 cut(s) 1516, 2059
BsuRI GGCC 8 cut(s) 453, 1484, 1558, 1593, 1653, 2454, 2518, 2804
BtgI CCRYGG 2 cut(s) 58, 2611
BtsCI GGATG 5 cut(s) 30, 685, 705, 714, 826
BtsI GCAGTG 3 cut(s) 746, 906, 1948
Cac8I GCNNGC 5 cut(s) 214, 1482, 2099, 2673, 2677
CaiI CAGNNNCTG 2 cut(s) 1144, 2865
CciI TCATGA 1 cut(s) 1071
CfoI GCGC 3 cut(s) 561, 1724, 2595
Cfr13I GGNCC 6 cut(s) 1381, 2452, 2453, 2516, 2741, 2802
CseI GACGC 2 cut(s) 1101, 1984
Csp6I GTAC 7 cut(s) 109, 921, 1151, 1311, 1363, 1953, 2009
CviQI GTAC 7 cut(s) 109, 921, 1151, 1311, 1363, 1953, 2009
DdeI CTNAG 8 cut(s) 293, 383, 1079, 1464, 1476, 1551, 1752, 2651
DpnI GATC 4 cut(s) 186, 1254, 1959, 2361
DpnII GATC 4 cut(s) 184, 1252, 1957, 2359
DraIII CACNNNGTG 2 cut(s) 1373, 1519
DrdI GACNNNNNNGTC 1 cut(s) 602
DseDI GACNNNNNNGTC 1 cut(s) 602
EaeI YGGCCR 2 cut(s) 1591, 1651
Eam1104I CTCTTC 6 cut(s) 86, 300, 1293, 2019, 2133, 2552
EarI CTCTTC 6 cut(s) 86, 300, 1293, 2019, 2133, 2552
Ecl136II GAGCTC 1 cut(s) 1279
Eco130I CCWWGG 7 cut(s) 58, 154, 1588, 1684, 2077, 2119, 2611
Eco24I GRGCYC 3 cut(s) 939, 1281, 2456
Eco31I GGTCTC 2 cut(s) 576, 2849
Eco32I GATATC 2 cut(s) 821, 1578
Eco47I GGWCC 2 cut(s) 1381, 2741
Eco53kI GAGCTC 1 cut(s) 1279
Eco57I CTGAAG 6 cut(s) 430, 551, 1311, 1797, 2157, 2508
Eco88I CYCGRG 1 cut(s) 336
Eco91I GGTNACC 1 cut(s) 593
EcoICRI GAGCTC 1 cut(s) 1279
EcoO65I GGTNACC 1 cut(s) 593
EcoRII CCWGG 9 cut(s) 609, 1143, 1191, 1248, 1597, 2218, 2288, 2817, 2858
EcoRV GATATC 2 cut(s) 821, 1578
EcoT14I CCWWGG 7 cut(s) 58, 154, 1588, 1684, 2077, 2119, 2611
EcoT22I ATGCAT 4 cut(s) 359, 2103, 2200, 2673
EcoT38I GRGCYC 3 cut(s) 939, 1281, 2456
ErhI CCWWGG 7 cut(s) 58, 154, 1588, 1684, 2077, 2119, 2611
Esp3I CGTCTC 2 cut(s) 1084, 2477
FalI AAGNNNNNCTT 1 cut(s) 31
FaqI GGGAC 1 cut(s) 1334
FauNDI CATATG 1 cut(s) 2797
FbaI TGATCA 1 cut(s) 1957
FblI GTMKAC 1 cut(s) 2406
Fnu4HI GCNGC 4 cut(s) 1139, 2238, 2540, 2697
FokI GGATG 5 cut(s) 37, 672, 712, 721, 813
FriOI GRGCYC 3 cut(s) 939, 1281, 2456
Fsp4HI GCNGC 4 cut(s) 1139, 2238, 2540, 2697
FspBI CTAG 9 cut(s) 230, 518, 626, 960, 1685, 2120, 2231, 2276, 2301
GlaI GCGC 3 cut(s) 560, 1723, 2594
GluI GCNGC 4 cut(s) 1139, 2238, 2540, 2697
GsuI CTGGAG 2 cut(s) 640, 693
HaeIII GGCC 8 cut(s) 453, 1484, 1558, 1593, 1653, 2454, 2518, 2804
HapII CCGG 3 cut(s) 274, 2536, 2752
HgaI GACGC 2 cut(s) 1101, 1984
HhaI GCGC 3 cut(s) 561, 1724, 2595
Hin1I GRCGYC 1 cut(s) 1323
Hin6I GCGC 3 cut(s) 559, 1722, 2593
HinP1I GCGC 3 cut(s) 559, 1722, 2593
HincII GTYRAC 4 cut(s) 1867, 2225, 2270, 2295
HindII GTYRAC 4 cut(s) 1867, 2225, 2270, 2295
HinfI GANTC 9 cut(s) 1055, 1177, 1223, 1703, 1887, 1946, 2132, 2657, 2733
HpaII CCGG 3 cut(s) 274, 2536, 2752
HpyCH4III ACNGT 8 cut(s) 47, 599, 637, 1373, 1826, 1871, 2411, 2719
HpyCH4IV ACGT 6 cut(s) 540, 1183, 1244, 1323, 1735, 2470
HpyF10VI GCNNNNNNNGC 8 cut(s) 222, 520, 908, 943, 1477, 1667, 2615, 2685
HpyF3I CTNAG 8 cut(s) 293, 383, 1079, 1464, 1476, 1551, 1752, 2651
HpySE526I ACGT 6 cut(s) 540, 1183, 1244, 1323, 1735, 2470
Hsp92I GRCGYC 1 cut(s) 1323
HspAI GCGC 3 cut(s) 559, 1722, 2593
Kpn2I TCCGGA 1 cut(s) 273
Ksp22I TGATCA 1 cut(s) 1957
Kzo9I GATC 4 cut(s) 184, 1252, 1957, 2359
Lsp1109I GCAGC 3 cut(s) 1150, 2224, 2551
MaeI CTAG 9 cut(s) 230, 518, 626, 960, 1685, 2120, 2231, 2276, 2301
MaeII ACGT 6 cut(s) 540, 1183, 1244, 1323, 1735, 2470
MaeIII GTNAC 5 cut(s) 178, 464, 593, 640, 2654
MalI GATC 4 cut(s) 186, 1254, 1959, 2361
MboI GATC 4 cut(s) 184, 1252, 1957, 2359
MfeI CAATTG 1 cut(s) 1662
MflI RGATCY 2 cut(s) 184, 1252
MhlI GDGCHC 5 cut(s) 939, 1281, 1683, 1758, 2456
MlsI TGGCCA 2 cut(s) 1593, 1653
MluNI TGGCCA 2 cut(s) 1593, 1653
MlyI GAGTC 2 cut(s) 2141, 2651
MmeI TCCRAC 1 cut(s) 787
Mox20I TGGCCA 2 cut(s) 1593, 1653
Mph1103I ATGCAT 4 cut(s) 359, 2103, 2200, 2673
MroI TCCGGA 1 cut(s) 273
MscI TGGCCA 2 cut(s) 1593, 1653
MseI TTAA 7 cut(s) 114, 1200, 1218, 1403, 1647, 2054, 2187
MslI CAYNNNNRTG 2 cut(s) 362, 2480
Msp20I TGGCCA 2 cut(s) 1593, 1653
MspCI CTTAAG 2 cut(s) 1402, 2053
MspI CCGG 3 cut(s) 274, 2536, 2752
MunI CAATTG 1 cut(s) 1662
Mva1269I GAATGC 4 cut(s) 225, 292, 359, 662
MvaI CCWGG 9 cut(s) 611, 1145, 1193, 1250, 1599, 2220, 2290, 2819, 2860
MvnI CGCG 3 cut(s) 326, 1722, 2595
MwoI GCNNNNNNNGC 8 cut(s) 222, 520, 908, 943, 1477, 1667, 2615, 2685
NciI CCSGG 2 cut(s) 2536, 2753
NcoI CCATGG 2 cut(s) 58, 2611
NdeI CATATG 1 cut(s) 2797
NdeII GATC 4 cut(s) 184, 1252, 1957, 2359
NlaIV GGNNCC 4 cut(s) 936, 2454, 2742, 2803
NmuCI GTSAC 3 cut(s) 464, 593, 2654
NsiI ATGCAT 4 cut(s) 359, 2103, 2200, 2673
NspI RCATGY 6 cut(s) 367, 551, 2101, 2198, 2485, 2675
PaeI GCATGC 2 cut(s) 2101, 2675
PagI TCATGA 1 cut(s) 1071
PciI ACATGT 1 cut(s) 2481
PctI GAATGC 4 cut(s) 225, 292, 359, 662
PfeI GAWTC 7 cut(s) 1055, 1177, 1223, 1703, 1887, 1946, 2733
PflFI GACNNNGTC 1 cut(s) 2777
PflMI CCANNNNNTGG 1 cut(s) 1519
PfoI TCCNGGA 2 cut(s) 1143, 1248
PkrI GCNGC 4 cut(s) 1140, 2239, 2541, 2698
PleI GAGTC 2 cut(s) 2140, 2651
PpsI GAGTC 2 cut(s) 2140, 2651
Ppu21I YACGTR 1 cut(s) 541
PscI ACATGT 1 cut(s) 2481
PshBI ATTAAT 1 cut(s) 2187
PsiI TTATAA 1 cut(s) 74
Psp124BI GAGCTC 1 cut(s) 1281
Psp6I CCWGG 9 cut(s) 609, 1143, 1191, 1248, 1597, 2218, 2288, 2817, 2858
PspEI GGTNACC 1 cut(s) 593
PspGI CCWGG 9 cut(s) 609, 1143, 1191, 1248, 1597, 2218, 2288, 2817, 2858
PspN4I GGNNCC 4 cut(s) 936, 2454, 2742, 2803
PspOMI GGGCCC 1 cut(s) 2452
PspPI GGNCC 6 cut(s) 1381, 2452, 2453, 2516, 2741, 2802
PstI CTGCAG 1 cut(s) 1914
PstNI CAGNNNCTG 2 cut(s) 1144, 2865
PsuI RGATCY 2 cut(s) 184, 1252
PsyI GACNNNGTC 1 cut(s) 2777
RsaI GTAC 7 cut(s) 110, 922, 1152, 1312, 1364, 1954, 2010
RsaNI GTAC 7 cut(s) 109, 921, 1151, 1311, 1363, 1953, 2009
RseI CAYNNNNRTG 2 cut(s) 362, 2480
SacI GAGCTC 1 cut(s) 1281
SaqAI TTAA 7 cut(s) 114, 1200, 1218, 1403, 1647, 2054, 2187
SatI GCNGC 4 cut(s) 1139, 2238, 2540, 2697
Sau3AI GATC 4 cut(s) 184, 1252, 1957, 2359
Sau96I GGNCC 6 cut(s) 1381, 2452, 2453, 2516, 2741, 2802
SchI GAGTC 2 cut(s) 2141, 2651
SduI GDGCHC 5 cut(s) 939, 1281, 1683, 1758, 2456
SfcI CTRYAG 2 cut(s) 633, 1910
SinI GGWCC 2 cut(s) 1381, 2741
SmiMI CAYNNNNRTG 2 cut(s) 362, 2480
SmlI CTYRAG 4 cut(s) 1402, 2053, 2092, 2846
SmoI CTYRAG 4 cut(s) 1402, 2053, 2092, 2846
SphI GCATGC 2 cut(s) 2101, 2675
SsiI CCGC 5 cut(s) 324, 824, 1670, 1720, 2696
SspMI CTAG 9 cut(s) 230, 518, 626, 960, 1685, 2120, 2231, 2276, 2301
SstI GAGCTC 1 cut(s) 1281
StyI CCWWGG 7 cut(s) 58, 154, 1588, 1684, 2077, 2119, 2611
TaaI ACNGT 8 cut(s) 47, 599, 637, 1373, 1826, 1871, 2411, 2719
TaiI ACGT 6 cut(s) 543, 1186, 1247, 1326, 1738, 2473
TaqI TCGA 1 cut(s) 1275
TatI WGTACW 5 cut(s) 108, 920, 1310, 1362, 2008
TauI GCSGC 1 cut(s) 2699
TfiI GAWTC 7 cut(s) 1055, 1177, 1223, 1703, 1887, 1946, 2733
Tru1I TTAA 7 cut(s) 114, 1200, 1218, 1403, 1647, 2054, 2187
Tru9I TTAA 7 cut(s) 114, 1200, 1218, 1403, 1647, 2054, 2187
TseFI GTSAC 3 cut(s) 464, 593, 2654
TseI GCWGC 3 cut(s) 1138, 2237, 2539
Tsp45I GTSAC 3 cut(s) 464, 593, 2654
TspDTI ATGAA 9 cut(s) 41, 189, 992, 1088, 1556, 1730, 2037, 2357, 2649
TspGWI ACGGA 2 cut(s) 1410, 1777
Tth111I GACNNNGTC 1 cut(s) 2777
Van91I CCANNNNNTGG 1 cut(s) 1519
Vha464I CTTAAG 2 cut(s) 1402, 2053
VneI GTGCAC 1 cut(s) 1679
VpaK11BI GGWCC 2 cut(s) 1381, 2741
VspI ATTAAT 1 cut(s) 2187
XapI RAATTY 6 cut(s) 50, 895, 1204, 1831, 1853, 2035
XbaI TCTAGA 2 cut(s) 517, 625
XceI RCATGY 6 cut(s) 367, 551, 2101, 2198, 2485, 2675
XcmI CCANNNNNNNNNTGG 1 cut(s) 1256
XmaJI CCTAGG 2 cut(s) 1684, 2119
XmiI GTMKAC 1 cut(s) 2406
XspI CTAG 9 cut(s) 230, 518, 626, 960, 1685, 2120, 2231, 2276, 2301
ZraI GACGTC 1 cut(s) 1324
Zsp2I ATGCAT 4 cut(s) 359, 2103, 2200, 2673
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.