Rh5AG284200

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0002206
Physical Location & Seq
Reverse (-)
38535324 .. 38539022
3699 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG284200.1

Sequence Viewer

Length: 2115 bp
ATGTCAGCAGAAATCCCTCTCTGGATTTGGTTTTTTGCCCTTGGGGTTTCTTCGGACAATGAAGTTACAGATCTGATTGATTATGACAAAGAAGATGCAAGCATTTCAAGCATTCTAGTTGCCTCAATTCGTGATGCTGACCAACGAAAAGATAACTTCCGGAGAGGGAAGAATGCTCTCAGATATGTTGAAGAGATGATAAAGAATACCGCGTTTCCTCCTCGGGAAAGCATTGAAGAATGCATCAGCATGTATCTGTTCCCCAATCTCAGGGGCATGAAGCAAAAGGCTCGGTTTCTTGGCTATATGGTGAAGTCTATTTTACAGACCTTCAGAGGCCACAGGAAATGTGACAACAGAGAAGAATTGAAGAACAAGAGGTTTCACTTGGCTGGTGAGCGAGAGCTGAAGGCACACGTAGGACATGCAGGAAGGCGCATGGCAAAGGCTTTGCAGAGAGACCTTTGTGGTGACCGTGTTGTCCGCCTGGTTGAGCAATATCAAAATGCTACAGTAGTAACCAATGGCATCTCCAGAGCATTCTCAACTGGAGCGTGGTGTCATCCTTTCAAGAGGATGGAGAGGATGTCTGGTGTGGTGGTAAATGTTGGGAAAACAAATCCACTGCAAACAATGGTAGATATGAGGAAGACACGCCTCCAAGTTCAATACACGGGGAAGTTCGGAGATGCCACATATCCGCATCCTTCTCACTGGGGTAGGGTTTGCTTTATCTCAACCCCAGATGGTGAGAATTGTGGGCTTGTGAAAAATTTGGCTACCACTGCACTGGTAAGTACAAACATATTGGAGCCCATATTGCCCGACTTTTTCTCTAGTGGCATGGAGGAGCTGGTAGATGATACTTCAACATCACTTCATGGGAAGAATAAAGTTTTTCTCAATGGGGATTGGGTTGGCGTTTGTGAAGATTCCCATGATTTTGTCATGAAACTAAGGACATTGAGACGCAGGAAAAAGTTGCTACATCAGCTCCTGGAAGTACGCATATATTCTGATGCTGGAAGGATTCTACGTCCTTTCCTGGTAGTTAAGAATTTGAATAAGATTAAAGAATCAAAAGGGAAAAAACAAACTGAAGAAGAATCGAGCTCATTGGAGCTGAAGAAGAAGAGGATTGCTGTACAGCATGGGACGTCAAGCATCTATTTATGGAGGGGAAGTCAGCAGTCAAGTACACAAACTGTGAACTGGGACCTATCATTTTTGTTAGGCTTAAGTTGTGGTATCATTCCTTTTTCAAACCATGATAATGCAAGGAGGGTTCTATACCAGTCTCAGAAGCACTCTCAGCAGGCCATTGGTTTTTCTGCCACAAATCCTAACCACAGAGTGGATACTCTGTCTCACCAACTACACTACCCTCAGAGGCCACTTTTTCGTACTATGATATCTGATTGCCTTGGCCAACCAGGAAACCCAGTGAGCCATAACAGAGTTATGCCCAAGCCTGAATACTTTAATGGCCAAAATGCAATTGTTGTGGTCAATGTGCACCTAGGATATAACCAAGAAGATTCTATTGTGATGAACCGCGCATCTGTGCAACGTGGTATGTTCAAATCTGAGCACATACGGAGCTACAAGGCTGAAGTTGAAAACAAGTTCTCATTGGGAAAAAGGAGGAAGCCAGATGACTGTATAAATTTTGGCAAAATACAGAGTAAATTTGGAAGTGTTGACAGTCTTGATGATGATGGATTCCCCTGTATAGGTGCATATCTGCAGAGTGGTGATACTATTATTGCGAGGTGCAGTGAATCTGGTACTGATCATAGCATCAAACTGAGGCATACTGAAAGAGGAAAGGTTCAGAAGGTTCGTTCTCCTTGCCTTGGAGACAAGTTCTCAAGCATGCATGGGCAGAAGGGTGTCCTAGGCTTTTTGGAGTCTGAAGAGAACTTCCCCTTCACAATGCAGGGGATAGTTCCAGATATTGTAATTAATCCACATGCATTTCCTTCACGACAAGCTCCTGGTCAACTCCTAGAGGCTGCTTTAGGGAAGGGGATTGCCTGTGGTGGCTCAATGAGATATGCAACTCCTTTCTCTACTCCCTCTGTTGATGACATCACAGATCAGCTTCATAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000228 GO:0000418 GO:0000419 GO:0000428 GO:0000785 GO:0000790 GO:0000792 GO:0002682 GO:0003674 GO:0003824 GO:0003899 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005720 GO:0005730 GO:0005736 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009605 GO:0009607 GO:0009620 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016020 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019222 GO:0019438 GO:0030422 GO:0030880 GO:0031047 GO:0031050 GO:0031974 GO:0031981 GO:0032259 GO:0032774 GO:0032991 GO:0034062 GO:0034641 GO:0034654 GO:0035194 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043331 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044451 GO:0044452 GO:0044454 GO:0044464 GO:0044728 GO:0046483 GO:0048519 GO:0048583 GO:0050776 GO:0050789 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0055029 GO:0060255 GO:0061695 GO:0065007 GO:0070013 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0097747 GO:0098542 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1901698 GO:1901699 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko01100 ko03020 ko05016 ko05169 map00230 map00240 map01100 map03020 map05016 map05169
Pfam Domains
Protein Families
RNA_pol_Rpb2_1 RNA_pol_Rpb2_2 RNA_pol_Rpb2_3 RNA_pol_Rpb2_4 RNA_pol_Rpb2_5 RNA_pol_Rpb2_6 RNA_pol_Rpb2_7

Protein Analysis

704

Amino Acids

79.46

Weight (kDa)

9.35

Isoelectric Point (pI)

44.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNA_pol_Rpb2_2 PF04561 3 - 127 1.3e-07 RNA polymerase Rpb2, domain 2
RNA_pol_Rpb2_3 PF04565 205 - 264 7.6e-15 RNA polymerase Rpb2, domain 3
RNA_pol_Rpb2_4 PF04566 299 - 352 5.7e-14 RNA polymerase Rpb2, domain 4
RNA_pol_Rpb2_6 PF00562 415 - 609 1.3e-33 RNA polymerase Rpb2, domain 6
RNA_pol_Rpb2_6 PF00562 610 - 704 2.8e-32 RNA polymerase Rpb2, domain 6
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 479
AatII GACGTC 1 cut(s) 1160
AccB7I CCANNNNNTGG 1 cut(s) 1354
AccII CGCG 2 cut(s) 212, 1557
AccIII TCCGGA 1 cut(s) 159
AciI CCGC 4 cut(s) 210, 484, 701, 1555
AcoI YGGCCR 2 cut(s) 1426, 1486
AcsI RAATTY 4 cut(s) 772, 1057, 1666, 1688
AcuI CTGAAG 6 cut(s) 316, 428, 1119, 1145, 1632, 1935
AcyI GRCGYC 1 cut(s) 1157
AdeI CACNNNGTG 1 cut(s) 1354
AfaI GTAC 6 cut(s) 799, 1005, 1146, 1198, 1405, 1789
AfiI CCNNNNNNNGG 4 cut(s) 270, 1354, 1733, 1856
AflII CTTAAG 1 cut(s) 1237
AflIII ACRYGT 1 cut(s) 415
AjnI CCWGG 5 cut(s) 486, 996, 1044, 1432, 1996
AluBI AGCT 8 cut(s) 406, 853, 994, 1113, 1123, 1602, 1994, 2104
AluI AGCT 8 cut(s) 406, 853, 994, 1113, 1123, 1602, 1994, 2104
Alw21I GWGCWC 3 cut(s) 1115, 1518, 1593
Alw26I GTCTC 5 cut(s) 453, 961, 1302, 1371, 1854
Alw44I GTGCAC 1 cut(s) 1514
AlwNI CAGNNNCTG 1 cut(s) 997
Ama87I CYCGRG 1 cut(s) 222
Aor13HI TCCGGA 1 cut(s) 159
AoxI GGCC 5 cut(s) 337, 1317, 1391, 1426, 1486
ApaLI GTGCAC 1 cut(s) 1514
ApeKI GCWGC 1 cut(s) 2015
ApoI RAATTY 4 cut(s) 772, 1057, 1666, 1688
AseI ATTAAT 1 cut(s) 1965
AspA2I CCTAGG 2 cut(s) 1519, 1897
AspLEI GCGC 2 cut(s) 438, 1559
AspS9I GGNCC 1 cut(s) 1216
AsuHPI GGTGA 6 cut(s) 322, 407, 482, 761, 1361, 1766
AvaI CYCGRG 1 cut(s) 222
AvaII GGWCC 1 cut(s) 1216
AvrII CCTAGG 2 cut(s) 1519, 1897
BaeGI GKGCMC 1 cut(s) 1518
BalI TGGCCA 2 cut(s) 1428, 1488
BanII GRGCYC 2 cut(s) 816, 1115
BbsI GAAGAC 1 cut(s) 656
Bbv12I GWGCWC 3 cut(s) 1115, 1518, 1593
BbvI GCAGC 1 cut(s) 2002
BccI CCATC 3 cut(s) 571, 740, 1712
BcgI CGANNNNNNTGC 2 cut(s) 272, 306
BciT130I CCWGG 5 cut(s) 488, 998, 1046, 1434, 1998
BciVI GTATCC 1 cut(s) 1351
BclI TGATCA 1 cut(s) 1792
BcoDI GTCTC 5 cut(s) 453, 961, 1302, 1371, 1854
BfaI CTAG 5 cut(s) 116, 837, 1520, 1898, 2009
BfmI CTRYAG 2 cut(s) 510, 1745
BfrI CTTAAG 1 cut(s) 1237
BfuI GTATCC 1 cut(s) 1351
BglII AGATCT 1 cut(s) 70
BisI GCNGC 1 cut(s) 2016
BlnI CCTAGG 2 cut(s) 1519, 1897
BlsI GCNGC 1 cut(s) 2017
Bme1390I CCNGG 5 cut(s) 488, 998, 1046, 1434, 1998
Bme18I GGWCC 1 cut(s) 1216
BmeT110I CYCGRG 1 cut(s) 222
BmgT120I GGNCC 1 cut(s) 1216
BmiI GGNNCC 2 cut(s) 813, 1217
BmrFI CCNGG 5 cut(s) 488, 998, 1046, 1434, 1998
BmrI ACTGGG 3 cut(s) 724, 1222, 1436
BmuI ACTGGG 3 cut(s) 724, 1222, 1436
BpiI GAAGAC 1 cut(s) 656
BpmI CTGGAG 2 cut(s) 517, 570
BpuEI CTTGAG 1 cut(s) 1855
BsaAI YACGTR 1 cut(s) 418
BsaHI GRCGYC 1 cut(s) 1157
BsaI GGTCTC 1 cut(s) 453
BsaJI CCNNGG 6 cut(s) 40, 221, 1423, 1519, 1855, 1897
BsaWI WCCGGW 1 cut(s) 159
BsaXI ACNNNNNCTCC 6 cut(s) 572, 602, 978, 1008, 1168, 1198
Bsc4I CCNNNNNNNGG 4 cut(s) 270, 1354, 1733, 1856
Bse1I ACTGG 6 cut(s) 553, 719, 795, 1217, 1294, 1442
BseAI TCCGGA 1 cut(s) 159
BseBI CCWGG 5 cut(s) 488, 998, 1046, 1434, 1998
BseDI CCNNGG 6 cut(s) 40, 221, 1423, 1519, 1855, 1897
BseGI GGATG 4 cut(s) 562, 582, 591, 703
BseLI CCNNNNNNNGG 4 cut(s) 270, 1354, 1733, 1856
BseMII CTCAG 7 cut(s) 193, 283, 1313, 1325, 1400, 1578, 1799
BseNI ACTGG 6 cut(s) 553, 719, 795, 1217, 1294, 1442
BseRI GAGGAG 2 cut(s) 210, 863
BseSI GKGCMC 1 cut(s) 1518
BseXI GCAGC 1 cut(s) 2002
BsgI GTGCAG 2 cut(s) 771, 1795
Bsh1236I CGCG 2 cut(s) 212, 1557
BshFI GGCC 5 cut(s) 339, 1319, 1393, 1428, 1488
BsiHKAI GWGCWC 3 cut(s) 1115, 1518, 1593
BsiHKCI CYCGRG 1 cut(s) 222
BsiSI CCGG 1 cut(s) 160
BslFI GGGAC 2 cut(s) 1168, 1229
BslI CCNNNNNNNGG 4 cut(s) 270, 1354, 1733, 1856
BsmAI GTCTC 5 cut(s) 453, 961, 1302, 1371, 1854
BsmBI CGTCTC 1 cut(s) 961
BsmFI GGGAC 2 cut(s) 1168, 1229
BsmI GAATGC 4 cut(s) 111, 178, 245, 539
BsnI GGCC 5 cut(s) 339, 1319, 1393, 1428, 1488
Bso31I GGTCTC 1 cut(s) 453
BsoBI CYCGRG 1 cut(s) 222
Bsp1286I GDGCHC 4 cut(s) 816, 1115, 1518, 1593
Bsp13I TCCGGA 1 cut(s) 159
Bsp1407I TGTACA 1 cut(s) 1144
Bsp143I GATC 3 cut(s) 70, 1792, 2098
BspACI CCGC 4 cut(s) 210, 484, 701, 1555
BspANI GGCC 5 cut(s) 339, 1319, 1393, 1428, 1488
BspCNI CTCAG 7 cut(s) 192, 282, 1312, 1324, 1399, 1579, 1800
BspEI TCCGGA 1 cut(s) 159
BspFNI CGCG 2 cut(s) 212, 1557
BspHI TCATGA 1 cut(s) 948
BspLI GGNNCC 2 cut(s) 813, 1217
BspMAI CTGCAG 1 cut(s) 1749
BspTI CTTAAG 1 cut(s) 1237
BspTNI GGTCTC 1 cut(s) 453
BsrGI TGTACA 1 cut(s) 1144
BsrI ACTGG 6 cut(s) 553, 719, 795, 1217, 1294, 1442
BssECI CCNNGG 6 cut(s) 40, 221, 1423, 1519, 1855, 1897
BssMI GATC 3 cut(s) 70, 1792, 2098
BssNI GRCGYC 1 cut(s) 1157
BssT1I CCWWGG 5 cut(s) 40, 1423, 1519, 1855, 1897
Bst2UI CCWGG 5 cut(s) 488, 998, 1046, 1434, 1998
Bst4CI ACNGT 5 cut(s) 476, 514, 1207, 1661, 1706
Bst6I CTCTTC 3 cut(s) 186, 1127, 1911
BstACI GRCGYC 1 cut(s) 1157
BstAFI CTTAAG 1 cut(s) 1237
BstAUI TGTACA 1 cut(s) 1144
BstBAI YACGTR 1 cut(s) 418
BstC8I GCNNGC 3 cut(s) 100, 1317, 1877
BstDEI CTNAG 8 cut(s) 179, 269, 956, 1299, 1311, 1386, 1587, 1808
BstEII GGTNACC 1 cut(s) 470
BstF5I GGATG 4 cut(s) 562, 582, 591, 703
BstFNI CGCG 2 cut(s) 212, 1557
BstHHI GCGC 2 cut(s) 438, 1559
BstKTI GATC 3 cut(s) 73, 1795, 2101
BstMAI GTCTC 5 cut(s) 453, 961, 1302, 1371, 1854
BstMBI GATC 3 cut(s) 70, 1792, 2098
BstMWI GCNNNNNNNGC 5 cut(s) 108, 785, 820, 991, 1312
BstNI CCWGG 5 cut(s) 488, 998, 1046, 1434, 1998
BstNSI RCATGY 4 cut(s) 253, 428, 1879, 1976
BstPI GGTNACC 1 cut(s) 470
BstSCI CCNGG 5 cut(s) 486, 996, 1044, 1432, 1996
BstSFI CTRYAG 2 cut(s) 510, 1745
BstSLI GKGCMC 1 cut(s) 1518
BstUI CGCG 2 cut(s) 212, 1557
BstV1I GCAGC 1 cut(s) 2002
BstV2I GAAGAC 1 cut(s) 656
BstX2I RGATCY 1 cut(s) 70
BstXI CCANNNNNNTGG 1 cut(s) 790
BstYI RGATCY 1 cut(s) 70
BsuI GTATCC 1 cut(s) 1351
BsuRI GGCC 5 cut(s) 339, 1319, 1393, 1428, 1488
BtsCI GGATG 4 cut(s) 562, 582, 591, 703
BtsI GCAGTG 3 cut(s) 623, 783, 1783
BtsIMutI CAGTG 6 cut(s) 623, 712, 783, 788, 1449, 1783
Cac8I GCNNGC 3 cut(s) 100, 1317, 1877
CaiI CAGNNNCTG 1 cut(s) 997
CciI TCATGA 1 cut(s) 948
CfoI GCGC 2 cut(s) 438, 1559
Cfr13I GGNCC 1 cut(s) 1216
CseI GACGC 1 cut(s) 978
Csp6I GTAC 6 cut(s) 798, 1004, 1145, 1197, 1404, 1788
CspCI CAANNNNNGTGG 2 cut(s) 1485, 1520
CviQI GTAC 6 cut(s) 798, 1004, 1145, 1197, 1404, 1788
DdeI CTNAG 8 cut(s) 179, 269, 956, 1299, 1311, 1386, 1587, 1808
DpnI GATC 3 cut(s) 72, 1794, 2100
DpnII GATC 3 cut(s) 70, 1792, 2098
DraIII CACNNNGTG 1 cut(s) 1354
DrdI GACNNNNNNGTC 1 cut(s) 479
DseDI GACNNNNNNGTC 1 cut(s) 479
EaeI YGGCCR 2 cut(s) 1426, 1486
Eam1104I CTCTTC 3 cut(s) 186, 1127, 1911
EarI CTCTTC 3 cut(s) 186, 1127, 1911
EciI GGCGGA 1 cut(s) 473
Ecl136II GAGCTC 1 cut(s) 1113
Eco130I CCWWGG 5 cut(s) 40, 1423, 1519, 1855, 1897
Eco24I GRGCYC 2 cut(s) 816, 1115
Eco31I GGTCTC 1 cut(s) 453
Eco32I GATATC 1 cut(s) 1413
Eco47I GGWCC 1 cut(s) 1216
Eco53kI GAGCTC 1 cut(s) 1113
Eco57I CTGAAG 6 cut(s) 316, 428, 1119, 1145, 1632, 1935
Eco88I CYCGRG 1 cut(s) 222
Eco91I GGTNACC 1 cut(s) 470
EcoICRI GAGCTC 1 cut(s) 1113
EcoO109I RGGNCCY 1 cut(s) 1216
EcoO65I GGTNACC 1 cut(s) 470
EcoRII CCWGG 5 cut(s) 486, 996, 1044, 1432, 1996
EcoRV GATATC 1 cut(s) 1413
EcoT14I CCWWGG 5 cut(s) 40, 1423, 1519, 1855, 1897
EcoT22I ATGCAT 3 cut(s) 245, 1881, 1978
EcoT38I GRGCYC 2 cut(s) 816, 1115
ErhI CCWWGG 5 cut(s) 40, 1423, 1519, 1855, 1897
Esp3I CGTCTC 1 cut(s) 961
FaqI GGGAC 2 cut(s) 1168, 1229
FbaI TGATCA 1 cut(s) 1792
Fnu4HI GCNGC 1 cut(s) 2016
FokI GGATG 4 cut(s) 549, 589, 598, 690
FriOI GRGCYC 2 cut(s) 816, 1115
Fsp4HI GCNGC 1 cut(s) 2016
FspBI CTAG 5 cut(s) 116, 837, 1520, 1898, 2009
GlaI GCGC 2 cut(s) 437, 1558
GluI GCNGC 1 cut(s) 2016
GsuI CTGGAG 2 cut(s) 517, 570
HaeIII GGCC 5 cut(s) 339, 1319, 1393, 1428, 1488
HapII CCGG 1 cut(s) 160
HgaI GACGC 1 cut(s) 978
HhaI GCGC 2 cut(s) 438, 1559
Hin1I GRCGYC 1 cut(s) 1157
Hin6I GCGC 2 cut(s) 436, 1557
HinP1I GCGC 2 cut(s) 436, 1557
HincII GTYRAC 2 cut(s) 1702, 2003
HindII GTYRAC 2 cut(s) 1702, 2003
HinfI GANTC 8 cut(s) 932, 1030, 1076, 1106, 1538, 1722, 1781, 1910
HpaII CCGG 1 cut(s) 160
HphI GGTGA 6 cut(s) 322, 407, 482, 761, 1361, 1766
Hpy166II GTNNAC 5 cut(s) 1199, 1210, 1516, 1702, 2003
Hpy8I GTNNAC 5 cut(s) 1199, 1210, 1516, 1702, 2003
HpyCH4III ACNGT 5 cut(s) 476, 514, 1207, 1661, 1706
HpyCH4IV ACGT 4 cut(s) 417, 1036, 1157, 1570
HpyF10VI GCNNNNNNNGC 5 cut(s) 108, 785, 820, 991, 1312
HpyF3I CTNAG 8 cut(s) 179, 269, 956, 1299, 1311, 1386, 1587, 1808
HpySE526I ACGT 4 cut(s) 417, 1036, 1157, 1570
Hsp92I GRCGYC 1 cut(s) 1157
HspAI GCGC 2 cut(s) 436, 1557
Kpn2I TCCGGA 1 cut(s) 159
Ksp22I TGATCA 1 cut(s) 1792
Kzo9I GATC 3 cut(s) 70, 1792, 2098
LmnI GCTCC 7 cut(s) 551, 811, 850, 999, 1120, 1599, 1999
Lsp1109I GCAGC 1 cut(s) 2002
MaeI CTAG 5 cut(s) 116, 837, 1520, 1898, 2009
MaeII ACGT 4 cut(s) 417, 1036, 1157, 1570
MaeIII GTNAC 4 cut(s) 64, 350, 470, 517
MalI GATC 3 cut(s) 72, 1794, 2100
MboI GATC 3 cut(s) 70, 1792, 2098
MfeI CAATTG 1 cut(s) 1497
MflI RGATCY 1 cut(s) 70
MhlI GDGCHC 4 cut(s) 816, 1115, 1518, 1593
MlsI TGGCCA 2 cut(s) 1428, 1488
MluCI AATT 9 cut(s) 126, 365, 754, 772, 1057, 1497, 1666, 1688, 1962
MluNI TGGCCA 2 cut(s) 1428, 1488
MlyI GAGTC 1 cut(s) 1919
Mox20I TGGCCA 2 cut(s) 1428, 1488
Mph1103I ATGCAT 3 cut(s) 245, 1881, 1978
MroI TCCGGA 1 cut(s) 159
MscI TGGCCA 2 cut(s) 1428, 1488
MseI TTAA 5 cut(s) 1053, 1071, 1238, 1482, 1965
MslI CAYNNNNRTG 2 cut(s) 248, 1272
Msp20I TGGCCA 2 cut(s) 1428, 1488
MspCI CTTAAG 1 cut(s) 1237
MspI CCGG 1 cut(s) 160
MspR9I CCNGG 5 cut(s) 488, 998, 1046, 1434, 1998
MunI CAATTG 1 cut(s) 1497
Mva1269I GAATGC 4 cut(s) 111, 178, 245, 539
MvaI CCWGG 5 cut(s) 488, 998, 1046, 1434, 1998
MvnI CGCG 2 cut(s) 212, 1557
MwoI GCNNNNNNNGC 5 cut(s) 108, 785, 820, 991, 1312
NdeII GATC 3 cut(s) 70, 1792, 2098
NlaIV GGNNCC 2 cut(s) 813, 1217
NmuCI GTSAC 2 cut(s) 350, 470
NsiI ATGCAT 3 cut(s) 245, 1881, 1978
NspI RCATGY 4 cut(s) 253, 428, 1879, 1976
PaeI GCATGC 1 cut(s) 1879
PagI TCATGA 1 cut(s) 948
PctI GAATGC 4 cut(s) 111, 178, 245, 539
PfeI GAWTC 7 cut(s) 932, 1030, 1076, 1106, 1538, 1722, 1781
PflMI CCANNNNNTGG 1 cut(s) 1354
PfoI TCCNGGA 1 cut(s) 996
PkrI GCNGC 1 cut(s) 2017
PleI GAGTC 1 cut(s) 1918
PpsI GAGTC 1 cut(s) 1918
Ppu21I YACGTR 1 cut(s) 418
PpuMI RGGWCCY 1 cut(s) 1216
PshBI ATTAAT 1 cut(s) 1965
Psp124BI GAGCTC 1 cut(s) 1115
Psp5II RGGWCCY 1 cut(s) 1216
Psp6I CCWGG 5 cut(s) 486, 996, 1044, 1432, 1996
PspEI GGTNACC 1 cut(s) 470
PspGI CCWGG 5 cut(s) 486, 996, 1044, 1432, 1996
PspN4I GGNNCC 2 cut(s) 813, 1217
PspPI GGNCC 1 cut(s) 1216
PspPPI RGGWCCY 1 cut(s) 1216
PstI CTGCAG 1 cut(s) 1749
PstNI CAGNNNCTG 1 cut(s) 997
PsuI RGATCY 1 cut(s) 70
RsaI GTAC 6 cut(s) 799, 1005, 1146, 1198, 1405, 1789
RsaNI GTAC 6 cut(s) 798, 1004, 1145, 1197, 1404, 1788
RseI CAYNNNNRTG 2 cut(s) 248, 1272
SacI GAGCTC 1 cut(s) 1115
SaqAI TTAA 5 cut(s) 1053, 1071, 1238, 1482, 1965
SatI GCNGC 1 cut(s) 2016
Sau3AI GATC 3 cut(s) 70, 1792, 2098
Sau96I GGNCC 1 cut(s) 1216
SchI GAGTC 1 cut(s) 1919
ScrFI CCNGG 5 cut(s) 488, 998, 1046, 1434, 1998
SduI GDGCHC 4 cut(s) 816, 1115, 1518, 1593
SfcI CTRYAG 2 cut(s) 510, 1745
SinI GGWCC 1 cut(s) 1216
SmiMI CAYNNNNRTG 2 cut(s) 248, 1272
SmlI CTYRAG 2 cut(s) 1237, 1870
SmoI CTYRAG 2 cut(s) 1237, 1870
SphI GCATGC 1 cut(s) 1879
Sse9I AATT 9 cut(s) 126, 365, 754, 772, 1057, 1497, 1666, 1688, 1962
SsiI CCGC 4 cut(s) 210, 484, 701, 1555
SspMI CTAG 5 cut(s) 116, 837, 1520, 1898, 2009
SstI GAGCTC 1 cut(s) 1115
StyD4I CCNGG 5 cut(s) 486, 996, 1044, 1432, 1996
StyI CCWWGG 5 cut(s) 40, 1423, 1519, 1855, 1897
TaaI ACNGT 5 cut(s) 476, 514, 1207, 1661, 1706
TaiI ACGT 4 cut(s) 420, 1039, 1160, 1573
TaqI TCGA 1 cut(s) 1109
TasI AATT 9 cut(s) 126, 365, 754, 772, 1057, 1497, 1666, 1688, 1962
TatI WGTACW 3 cut(s) 797, 1144, 1196
TfiI GAWTC 7 cut(s) 932, 1030, 1076, 1106, 1538, 1722, 1781
Tru1I TTAA 5 cut(s) 1053, 1071, 1238, 1482, 1965
Tru9I TTAA 5 cut(s) 1053, 1071, 1238, 1482, 1965
TscAI CASTG 6 cut(s) 630, 719, 790, 795, 1449, 1783
TseFI GTSAC 2 cut(s) 350, 470
TseI GCWGC 1 cut(s) 2015
Tsp45I GTSAC 2 cut(s) 350, 470
TspDTI ATGAA 6 cut(s) 75, 293, 869, 965, 1565, 2096
TspGWI ACGGA 1 cut(s) 1612
TspRI CASTG 6 cut(s) 630, 719, 790, 795, 1449, 1783
Van91I CCANNNNNTGG 1 cut(s) 1354
Vha464I CTTAAG 1 cut(s) 1237
VneI GTGCAC 1 cut(s) 1514
VpaK11BI GGWCC 1 cut(s) 1216
VspI ATTAAT 1 cut(s) 1965
XapI RAATTY 4 cut(s) 772, 1057, 1666, 1688
XceI RCATGY 4 cut(s) 253, 428, 1879, 1976
XmaJI CCTAGG 2 cut(s) 1519, 1897
XspI CTAG 5 cut(s) 116, 837, 1520, 1898, 2009
ZraI GACGTC 1 cut(s) 1158
Zsp2I ATGCAT 3 cut(s) 245, 1881, 1978
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.