MD08G1057300.v1.1

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0002206
Physical Location & Seq
Reverse (-)
4461823 .. 4469194
7372 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1057300.v1.1.491

Sequence Viewer

Length: 3576 bp
ATGAGCAGCAAGGGAGAAAGTTTTACAAATGGTGTTGACATGGACATCGACGATAGTGATTATGATGATGAGTATGATTACTCAACTAGCTTGCAAGATCTTGGGGAAGGATTTCTGAAGAATTTCTGTAAGGAGGCGGCAACATCATTCTTCAACGAGTATGGCCTCATTAGTCATCAAATCAACTCATATAATGACTTCATACAAAATGGCATACAAAGAGTCTTCAGTTCTTTTGGCGAGATTATAGTGGAGCCTGGCTATGACCCGTCAAAGAAGGGAGACAATGAATGGCGCTATGCTTTAGTGAAGTTTGGGAAGGTTACTCTTGGTAGGCCAAGTTTTTGGGGTGGTTCTGACAATGATAAGGAATATAATATGTTGCCTAGGCATGCTCGACTTCAGAACATGACATACTGCGCTAAGATGAAAGTGAATATCACTGTTGAGGTATATAATCAACGACTTGTCAGCAGCGATAAGTTTAAGACTGGAAAAGAACGGTTCCTTGACAAGGAAATTTTAAGTACGGATACCAGAGACATTACGATTGGTAGTGTCCCTGTAATGGTGAAGTCTGATTTATGCTGGATGAGGGACGTTGAGAAAGGTGACTGTGATTTTGACCATGGAGGCTATTTTATTATCAAGGGTGCAGAGAAGACATTTATTGCACAAGAACAGAGCTGTCTGAAGAAACTTCTCATTACAAATAATCAGGGTCTGACAGTAGCATATAGGTCAGAGGTGAAGAGGCATAGGTTAATAATTAGACTAGTTGGGATTTCTAAACTTGAAAACATTGAAGGAGTAGAGAAAGTCCTTACCGTTTACTTCATGTCGACAGAAATCCCTGTGTGGGTATGGTTTTTTGCCCTTGGTGTTTCATCGGATAAAGAAGTTATTGATCTGATTGATTATGGCAGTGAAGATGCCAGCATTTTGAATATACTTTTTGCCTCAATTCGCGATGCCGATAAAGCTACTGAAAAAGTGGATGGAGGCTTCCGTAGGGGGAAGAATGCCCTCAAATATGTGGATGATGTGATAAAGAAAACTGCATTTCCACCTGGGGAAAGCATGGAAGAGTGCATCAGCTTGTATCTTTTCCCCAATCTCAGAGGTCTAAAGCAAAAGGCTCGCTTTCTTGGGTATATGGTGAAGTCCCTGTTACAAGCCTCTGCTGGTCGCAGGAAATGTGACAATAGGGATGATTTTAGGAACAAGAGGTTGGACTTGGCGGGTGAGCTTCTCGAACGAGAGCTTAAGGCACATATTGGACATGCAAGGCGGCGCATGGCAAAGGCCTTGCAGAGAGACCTTTATGGTGATCGGGTTGTGCGTCCAATTGAACACTACCTAGATGCCTCCATAGTTACTAATGGCATTTCAAGAGCATTCTCAACTGGAGCATGGTGTCATCCCTTCAAGAAAATGGAGAGGATGTCTGGTGTAGTGGCAACAGTTGGGCGAGCAAATCCGCTGCAGACAATGATTGATATGCGGAAGACAAGGCAGCAGGTTCAATATACAGGGAAGGTTGGAGATGCCAGATACCCGCATCCTTCCCACTGGGGTAAAGTATGTTTTCTCTCAACTCCGGATGGTGAGAATTGTGGGCTTGTAAAAAATTTGGCTACCACTACAATAGTAAGTACGAATATATTGGAGTCTCTACTACCTGAATTGTCACTCTCTGGAATGGAAAAATTGGTGGATGATACTTCTACCTCACTGCAAGGGAAGTTCAAAGTTTTTCTGAATGGGGATTGGGTTGGAGTTTGTGAAGATTCCCTCTCATTTGTCTCAGAACTCAGGAGGATGCGACGTAGGAAAAAGTTGCCACCTCAGGTGGAAATCAAAAGAGATGAAAAGCAAGGAGAAGTACGAGTATATTCTGATGCTGGAAGGATCCTACGCCCTCTCTTGGTGGTTGAGAATTTGAACAAGATAAAAGCATTGAAAGGGGAAAAGCATCCATTCAAGTATTTGCTTAACAAAGGAATAATCGAGCTTATTGGAGCTGAAGAAGAGGAAGACTGTAGTACTGCATGGGGCATTAAATATCTGTTCATGGAAGAAAAGGGAAAATCAGCTGTAAAGTACACACATTGTGAGCTGGACATGTCATTCCTGTTGGGTTTGAGCTGTAGCATTATCCCATTTGCGAATCATGACCATGCAAGGAGGGTCCTCTACCAAGCACAGAAGCACTCGTCGCAGGCTATTGGGTTTTCTACCACAAATCCTAACCTTCGAGTGGATACACTGTCTCACCAACTGCACTACCCCCAGAGGTCACTTTTTCAGACAATGACGTCTGACTGCCTTGGCAAACCAGGACACCAACTGGGTAAAAACAGAGTTTTGCCGAAGCCCGAATTGTACAATGGTCAGAACGCGATTGTGGCAGTCAATGTTCACTTGGGGTTCAACCAAGAAGATAGCATAGTAATGAATCGGGCATCACTGGAACGTGGTATGTTCCGATCTGAACATATCAGAAGTTACAAGGCTGAGGTTGACAACAAGCACTCCCTGGAGAAAAGGCGGAAGCCTGATGATTGTGTCAATTTTGGCAAGATGCAGAGCAAGTTTGGTCGGGTTGACAATCTCGATGAAGACGGCTTTCCCTACGTTGGTGCAAACTTGCAGAGTGGCGATATCATCATTGGGAGGTGCAGTGAATCAGGAGCTGATCATAGCATCAAACTGAAGCACACTGAAAGGGGAATGGTTCAGAAGGTTGTGCTTTCTTCTAATGATGATGGAAAGAACTTTGCTGTTGTTTCTATGAGACAGGTTCGCTCACCGTGTCTTGGAGACAAGTTCTCCAGCATGCATGGGCAAAAGGGTGTTCTAGGTTTTCTGGAGTCTCAAGAGAACTTCCCTTTCACAGTACAGGGGATAGTTCCAGATATTGTGATAAATCCCCATGCATTTCCGTCAAGACAAACTCCTGGTCAACTCTTAGAAGCTGCTTTAGGAAAGGGAATCGCCTCTGGTGGTTCAAAGAAATATGCCACCCCGTTCTCTACTCTCTCTGTCGATGACATCGCAGAACAACTGCATAGGGCTGGATTTTCAAGATGCGGAAAAGAGAGAGTATACAATGGTGGAACCGGTAAAATGGCTCGTTCCCTCATATTCATGGGCCCAACCTTTTACCAGCGCCTGATTCACATGTCTGAAGACAAAGTAAAGTTCAGGAACACCGGGCCAGTCCACCCTCTCACCCGTCAACCAGTGGCAGATCGGAAGCGATTTGGAGGGATCAAGTTTGGAGAAATGGAACGTGACTGCCTAATAGCCCACGGAGCTTCTGCAAACTTACATGAGCGCCTTTTCACTCTCAGCGACTCCTCCCAGGTGTATATCTGCCAGAAATGCGAACATGTAGCTAATGTGATCCAACGATCGGTTGAAGGAGGACGGAAGATCAGGGGTCCCTACTGCCGGAATTGCAATTCTGCAGATGATCTTGTCAAGGCAAACGTCCCGTACGGAGCGAAGTTACTGTGCCAGGAGCTGTTCAGCATGGGCATCAGTTTGAAGTTCGAGACTCGGTTCGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000228 GO:0000418 GO:0000419 GO:0000428 GO:0000785 GO:0000790 GO:0000792 GO:0002682 GO:0003674 GO:0003824 GO:0003899 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005720 GO:0005730 GO:0005736 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009605 GO:0009607 GO:0009620 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016020 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019222 GO:0019438 GO:0030422 GO:0030880 GO:0031047 GO:0031050 GO:0031974 GO:0031981 GO:0032259 GO:0032774 GO:0032991 GO:0034062 GO:0034641 GO:0034654 GO:0035194 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043331 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044451 GO:0044452 GO:0044454 GO:0044464 GO:0044728 GO:0046483 GO:0048519 GO:0048583 GO:0050776 GO:0050789 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0055029 GO:0060255 GO:0061695 GO:0065007 GO:0070013 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0097747 GO:0098542 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1901698 GO:1901699 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko01100 ko03020 ko05016 ko05169 map00230 map00240 map01100 map03020 map05016 map05169
Pfam Domains
Protein Families
RNA_pol_Rpb2_1 RNA_pol_Rpb2_2 RNA_pol_Rpb2_3 RNA_pol_Rpb2_4 RNA_pol_Rpb2_5 RNA_pol_Rpb2_6 RNA_pol_Rpb2_7

Protein Analysis

1192

Amino Acids

134.11

Weight (kDa)

8.92

Isoelectric Point (pI)

37.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNA_pol_Rpb2_1 PF04563 55 - 443 3.1e-32 RNA polymerase beta subunit
RNA_pol_Rpb2_2 PF04561 268 - 412 7.4e-12 RNA polymerase Rpb2, domain 2
RNA_pol_Rpb2_3 PF04565 491 - 549 6.8e-15 RNA polymerase Rpb2, domain 3
RNA_pol_Rpb2_4 PF04566 585 - 646 1.9e-23 RNA polymerase Rpb2, domain 4
RNA_pol_Rpb2_5 PF04567 661 - 708 5.5e-07 RNA polymerase Rpb2, domain 5
RNA_pol_Rpb2_6 PF00562 717 - 1088 7e-108 RNA polymerase Rpb2, domain 6
RNA_pol_Rpb2_7 PF04560 1090 - 1187 6.9e-27 RNA polymerase Rpb2, domain 7
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2317
AatII GACGTC 1 cut(s) 2321
Acc36I ACCTGC 1 cut(s) 1510
AccI GTMKAC 2 cut(s) 842, 3108
AccII CGCG 2 cut(s) 969, 2402
AccIII TCCGGA 1 cut(s) 1600
AciI CCGC 8 cut(s) 137, 1241, 1291, 1481, 1504, 1559, 2551, 3093
AclWI GGATC 4 cut(s) 1906, 1919, 3281, 3403
AcsI RAATTY 4 cut(s) 121, 519, 1630, 1939
AcuI CTGAAG 7 cut(s) 137, 211, 386, 713, 2046, 2735, 3210
AcyI GRCGYC 1 cut(s) 2318
AdeI CACNNNGTG 1 cut(s) 2114
AfaI GTAC 8 cut(s) 529, 1657, 1887, 2047, 2105, 2387, 2901, 3503
AfiI CCNNNNNNNGG 9 cut(s) 514, 568, 859, 1927, 2260, 2639, 2819, 3418, 3456
AflII CTTAAG 1 cut(s) 1265
AflIII ACRYGT 3 cut(s) 2124, 3183, 3394
AgeI ACCGGT 1 cut(s) 3122
AhlI ACTAGT 1 cut(s) 775
AjnI CCWGG 7 cut(s) 256, 1069, 2338, 2538, 2959, 3366, 3522
AjuI GAANNNNNNNTTGG 2 cut(s) 1214, 1246
AlwI GGATC 4 cut(s) 1906, 1919, 3281, 3403
AlwNI CAGNNNCTG 4 cut(s) 724, 2696, 3175, 3529
Aor13HI TCCGGA 1 cut(s) 1600
AoxI GGCC 5 cut(s) 163, 335, 1305, 3154, 3218
ApaI GGGCCC 1 cut(s) 3158
ApeKI GCWGC 5 cut(s) 6, 474, 1483, 1516, 2978
ApoI RAATTY 4 cut(s) 121, 519, 1630, 1939
ArsI GACNNNNNNTTYG 4 cut(s) 605, 637, 2947, 2979
AsiGI ACCGGT 1 cut(s) 3122
Asp700I GAANNNNTTC 2 cut(s) 111, 122
AspA2I CCTAGG 1 cut(s) 386
AspLEI GCGC 5 cut(s) 297, 422, 1296, 3174, 3342
AspS9I GGNCC 5 cut(s) 2191, 3154, 3155, 3218, 3446
AsuC2I CCSGG 1 cut(s) 3217
AvaII GGWCC 2 cut(s) 2191, 3446
AvrII CCTAGG 1 cut(s) 386
AxyI CCTNAGG 1 cut(s) 1848
BaeGI GKGCMC 1 cut(s) 3158
BamHI GGATCC 1 cut(s) 1911
BanII GRGCYC 1 cut(s) 3158
BbsI GAAGAC 6 cut(s) 217, 668, 1514, 2043, 2628, 3198
BbvCI CCTCAGC 1 cut(s) 2517
BbvI GCAGC 5 cut(s) 18, 486, 1470, 1528, 2965
BccI CCATC 3 cut(s) 992, 1598, 2762
BceAI ACGGC 1 cut(s) 2641
BcgI CGANNNNNNTGC 2 cut(s) 1121, 1155
BciT130I CCWGG 7 cut(s) 258, 1071, 2340, 2540, 2961, 3368, 3524
BciVI GTATCC 2 cut(s) 526, 2257
BclI TGATCA 1 cut(s) 2698
BcnI CCSGG 1 cut(s) 3217
BcuI ACTAGT 1 cut(s) 775
BfaI CTAG 5 cut(s) 87, 387, 776, 1361, 2861
BfmI CTRYAG 4 cut(s) 1484, 2041, 2149, 3471
BfoI RGCGCY 3 cut(s) 298, 3175, 3343
BfrI CTTAAG 1 cut(s) 1265
BfuAI ACCTGC 1 cut(s) 1510
BfuI GTATCC 2 cut(s) 526, 2257
BglII AGATCT 1 cut(s) 97
BisI GCNGC 7 cut(s) 7, 138, 475, 1292, 1484, 1517, 2979
BlnI CCTAGG 1 cut(s) 386
BlsI GCNGC 7 cut(s) 8, 139, 476, 1293, 1485, 1518, 2980
BmcAI AGTACT 1 cut(s) 2047
Bme1390I CCNGG 8 cut(s) 258, 1071, 2340, 2540, 2961, 3217, 3368, 3524
Bme18I GGWCC 2 cut(s) 2191, 3446
BmgT120I GGNCC 5 cut(s) 2191, 3154, 3155, 3218, 3446
BmiI GGNNCC 8 cut(s) 255, 506, 1913, 2192, 3121, 3156, 3447, 3448
BmrFI CCNGG 8 cut(s) 258, 1071, 2340, 2540, 2961, 3217, 3368, 3524
BmrI ACTGGG 2 cut(s) 1582, 2360
BmuI ACTGGG 2 cut(s) 1582, 2360
BpiI GAAGAC 6 cut(s) 217, 668, 1514, 2043, 2628, 3198
BpmI CTGGAG 4 cut(s) 1428, 2561, 2818, 2891
Bpu10I CCTNAGC 1 cut(s) 2517
BpuEI CTTGAG 1 cut(s) 2862
BpuMI CCSGG 1 cut(s) 3217
BsaHI GRCGYC 1 cut(s) 2318
BsaI GGTCTC 1 cut(s) 1311
BsaJI CCNNGG 8 cut(s) 386, 628, 877, 1070, 2329, 2538, 3313, 3366
BsaWI WCCGGW 2 cut(s) 1600, 3122
BsaXI ACNNNNNCTCC 8 cut(s) 1430, 1460, 2519, 2549, 2668, 2698, 2816, 2846
Bsc4I CCNNNNNNNGG 9 cut(s) 514, 568, 859, 1927, 2260, 2639, 2819, 3418, 3456
Bse118I RCCGGY 1 cut(s) 3122
Bse1I ACTGG 7 cut(s) 496, 1411, 1577, 2355, 2475, 3221, 3245
Bse21I CCTNAGG 1 cut(s) 1848
BseAI TCCGGA 1 cut(s) 1600
BseBI CCWGG 7 cut(s) 258, 1071, 2340, 2540, 2961, 3368, 3524
BseDI CCNNGG 8 cut(s) 386, 628, 877, 1070, 2329, 2538, 3313, 3366
BseLI CCNNNNNNNGG 9 cut(s) 514, 568, 859, 1927, 2260, 2639, 2819, 3418, 3456
BseMII CTCAG 6 cut(s) 1132, 1821, 1828, 1862, 2508, 3367
BseNI ACTGG 7 cut(s) 496, 1411, 1577, 2355, 2475, 3221, 3245
BseRI GAGGAG 1 cut(s) 3352
BseSI GKGCMC 1 cut(s) 3158
BseXI GCAGC 5 cut(s) 18, 486, 1470, 1528, 2965
BsgI GTGCAG 3 cut(s) 675, 2267, 2701
Bsh1236I CGCG 2 cut(s) 969, 2402
Bsh1285I CGRYCG 1 cut(s) 3419
BshFI GGCC 5 cut(s) 165, 337, 1307, 3156, 3220
BshTI ACCGGT 1 cut(s) 3122
BsiEI CGRYCG 1 cut(s) 3419
BsiSI CCGG 4 cut(s) 1601, 3123, 3216, 3457
BsiWI CGTACG 1 cut(s) 3501
BslFI GGGAC 5 cut(s) 545, 611, 1150, 3432, 3482
BslI CCNNNNNNNGG 9 cut(s) 514, 568, 859, 1927, 2260, 2639, 2819, 3418, 3456
BsmFI GGGAC 5 cut(s) 545, 611, 1150, 3432, 3482
BsmI GAATGC 2 cut(s) 1027, 1397
BsnI GGCC 5 cut(s) 165, 337, 1307, 3156, 3220
Bso31I GGTCTC 1 cut(s) 1311
Bsp120I GGGCCC 1 cut(s) 3154
Bsp1286I GDGCHC 1 cut(s) 3158
Bsp13I TCCGGA 1 cut(s) 1600
Bsp1407I TGTACA 1 cut(s) 2385
Bsp19I CCATGG 1 cut(s) 628
Bsp68I TCGCGA 1 cut(s) 969
BspACI CCGC 8 cut(s) 137, 1241, 1291, 1481, 1504, 1559, 2551, 3093
BspANI GGCC 5 cut(s) 165, 337, 1307, 3156, 3220
BspCNI CTCAG 6 cut(s) 1131, 1820, 1827, 1861, 2509, 3366
BspEI TCCGGA 1 cut(s) 1600
BspFNI CGCG 2 cut(s) 969, 2402
BspHI TCATGA 1 cut(s) 2173
BspLI GGNNCC 8 cut(s) 255, 506, 1913, 2192, 3121, 3156, 3447, 3448
BspMAI CTGCAG 2 cut(s) 1488, 3475
BspMI ACCTGC 1 cut(s) 1510
BspPI GGATC 4 cut(s) 1906, 1919, 3281, 3403
BspTI CTTAAG 1 cut(s) 1265
BspTNI GGTCTC 1 cut(s) 1311
BsrFI RCCGGY 1 cut(s) 3122
BsrGI TGTACA 1 cut(s) 2385
BsrI ACTGG 7 cut(s) 496, 1411, 1577, 2355, 2475, 3221, 3245
BssAI RCCGGY 1 cut(s) 3122
BssECI CCNNGG 8 cut(s) 386, 628, 877, 1070, 2329, 2538, 3313, 3366
BssNAI GTATAC 1 cut(s) 3109
BssNI GRCGYC 1 cut(s) 2318
BssT1I CCWWGG 4 cut(s) 386, 628, 877, 2329
Bst1107I GTATAC 1 cut(s) 3109
Bst2UI CCWGG 7 cut(s) 258, 1071, 2340, 2540, 2961, 3368, 3524
Bst6I CTCTTC 3 cut(s) 746, 1080, 2025
BstACI GRCGYC 1 cut(s) 2318
BstAFI CTTAAG 1 cut(s) 1265
BstAUI TGTACA 1 cut(s) 2385
BstC8I GCNNGC 7 cut(s) 92, 393, 937, 1141, 1473, 2223, 2840
BstDEI CTNAG 9 cut(s) 423, 1118, 1807, 1814, 1848, 2517, 2971, 3353, 3573
BstDSI CCRYGG 2 cut(s) 628, 3313
BstENI CCTNNNNNAGG 1 cut(s) 512
BstFNI CGCG 2 cut(s) 969, 2402
BstH2I RGCGCY 3 cut(s) 298, 3175, 3343
BstHHI GCGC 5 cut(s) 297, 422, 1296, 3174, 3342
BstMCI CGRYCG 1 cut(s) 3419
BstMWI GCNNNNNNNGC 6 cut(s) 980, 2218, 2408, 3317, 3387, 3462
BstNI CCWGG 7 cut(s) 258, 1071, 2340, 2540, 2961, 3368, 3524
BstNSI RCATGY 6 cut(s) 395, 1286, 2128, 2842, 3187, 3398
BstSCI CCNGG 8 cut(s) 256, 1069, 2338, 2538, 2959, 3215, 3366, 3522
BstSFI CTRYAG 4 cut(s) 1484, 2041, 2149, 3471
BstSLI GKGCMC 1 cut(s) 3158
BstUI CGCG 2 cut(s) 969, 2402
BstV1I GCAGC 5 cut(s) 18, 486, 1470, 1528, 2965
BstV2I GAAGAC 6 cut(s) 217, 668, 1514, 2043, 2628, 3198
BstX2I RGATCY 2 cut(s) 97, 1911
BstXI CCANNNNNNTGG 1 cut(s) 345
BstYI RGATCY 2 cut(s) 97, 1911
BstZ17I GTATAC 1 cut(s) 3109
Bsu36I CCTNAGG 1 cut(s) 1848
BsuI GTATCC 2 cut(s) 526, 2257
BsuRI GGCC 5 cut(s) 165, 337, 1307, 3156, 3220
BtgI CCRYGG 2 cut(s) 628, 3313
BtgZI GCGATG 2 cut(s) 984, 3040
BtsI GCAGTG 3 cut(s) 931, 1733, 2689
BtsIMutI CAGTG 9 cut(s) 441, 931, 1570, 1733, 2267, 2468, 2689, 2721, 3252
BtuMI TCGCGA 1 cut(s) 969
BveI ACCTGC 1 cut(s) 1510
Cac8I GCNNGC 7 cut(s) 92, 393, 937, 1141, 1473, 2223, 2840
CaiI CAGNNNCTG 4 cut(s) 724, 2696, 3175, 3529
CciI TCATGA 1 cut(s) 2173
CfoI GCGC 5 cut(s) 297, 422, 1296, 3174, 3342
Cfr10I RCCGGY 1 cut(s) 3122
Cfr13I GGNCC 5 cut(s) 2191, 3154, 3155, 3218, 3446
CseI GACGC 1 cut(s) 1331
Csp6I GTAC 8 cut(s) 528, 1656, 1886, 2046, 2104, 2386, 2900, 3502
CspAI ACCGGT 1 cut(s) 3122
CviQI GTAC 8 cut(s) 528, 1656, 1886, 2046, 2104, 2386, 2900, 3502
DdeI CTNAG 9 cut(s) 423, 1118, 1807, 1814, 1848, 2517, 2971, 3353, 3573
DraIII CACNNNGTG 1 cut(s) 2114
DrdI GACNNNNNNGTC 1 cut(s) 2317
DseDI GACNNNNNNGTC 1 cut(s) 2317
Eam1104I CTCTTC 3 cut(s) 746, 1080, 2025
EarI CTCTTC 3 cut(s) 746, 1080, 2025
EciI GGCGGA 1 cut(s) 2566
Eco130I CCWWGG 4 cut(s) 386, 628, 877, 2329
Eco147I AGGCCT 1 cut(s) 1307
Eco24I GRGCYC 1 cut(s) 3158
Eco31I GGTCTC 1 cut(s) 1311
Eco32I GATATC 1 cut(s) 2665
Eco47I GGWCC 2 cut(s) 2191, 3446
Eco57I CTGAAG 7 cut(s) 137, 211, 386, 713, 2046, 2735, 3210
Eco81I CCTNAGG 1 cut(s) 1848
EcoNI CCTNNNNNAGG 1 cut(s) 512
EcoO109I RGGNCCY 2 cut(s) 2191, 3446
EcoRII CCWGG 7 cut(s) 256, 1069, 2338, 2538, 2959, 3366, 3522
EcoRV GATATC 1 cut(s) 2665
EcoT14I CCWWGG 4 cut(s) 386, 628, 877, 2329
EcoT22I ATGCAT 2 cut(s) 2844, 2941
EcoT38I GRGCYC 1 cut(s) 3158
ErhI CCWWGG 4 cut(s) 386, 628, 877, 2329
FalI AAGNNNNNCTT 2 cut(s) 1127, 1159
FaqI GGGAC 5 cut(s) 545, 611, 1150, 3432, 3482
FauI CCCGC 2 cut(s) 1234, 1566
FbaI TGATCA 1 cut(s) 2698
FblI GTMKAC 2 cut(s) 842, 3108
Fnu4HI GCNGC 7 cut(s) 7, 138, 475, 1292, 1484, 1517, 2979
FriOI GRGCYC 1 cut(s) 3158
Fsp4HI GCNGC 7 cut(s) 7, 138, 475, 1292, 1484, 1517, 2979
FspBI CTAG 5 cut(s) 87, 387, 776, 1361, 2861
GlaI GCGC 5 cut(s) 296, 421, 1295, 3173, 3341
GluI GCNGC 7 cut(s) 7, 138, 475, 1292, 1484, 1517, 2979
GsuI CTGGAG 4 cut(s) 1428, 2561, 2818, 2891
HaeII RGCGCY 3 cut(s) 298, 3175, 3343
HaeIII GGCC 5 cut(s) 165, 337, 1307, 3156, 3220
HapII CCGG 4 cut(s) 1601, 3123, 3216, 3457
HgaI GACGC 1 cut(s) 1331
HhaI GCGC 5 cut(s) 297, 422, 1296, 3174, 3342
Hin1I GRCGYC 1 cut(s) 2318
Hin6I GCGC 5 cut(s) 295, 420, 1294, 3172, 3340
HinP1I GCGC 5 cut(s) 295, 420, 1294, 3172, 3340
HincII GTYRAC 6 cut(s) 37, 843, 2524, 2608, 2966, 3242
HindII GTYRAC 6 cut(s) 37, 843, 2524, 2608, 2966, 3242
HpaII CCGG 4 cut(s) 1601, 3123, 3216, 3457
Hpy99I CGWCG 3 cut(s) 53, 1830, 2221
HpyCH4IV ACGT 7 cut(s) 600, 1828, 2318, 2476, 2637, 3295, 3495
HpyF10VI GCNNNNNNNGC 6 cut(s) 980, 2218, 2408, 3317, 3387, 3462
HpyF3I CTNAG 9 cut(s) 423, 1118, 1807, 1814, 1848, 2517, 2971, 3353, 3573
HpySE526I ACGT 7 cut(s) 600, 1828, 2318, 2476, 2637, 3295, 3495
Hsp92I GRCGYC 1 cut(s) 2318
HspAI GCGC 5 cut(s) 295, 420, 1294, 3172, 3340
KflI GGGWCCC 1 cut(s) 3446
Kpn2I TCCGGA 1 cut(s) 1600
Ksp22I TGATCA 1 cut(s) 2698
LmnI GCTCC 7 cut(s) 253, 1409, 2021, 2693, 3317, 3506, 3526
Lsp1109I GCAGC 5 cut(s) 18, 486, 1470, 1528, 2965
MaeI CTAG 5 cut(s) 87, 387, 776, 1361, 2861
MaeII ACGT 7 cut(s) 600, 1828, 2318, 2476, 2637, 3295, 3495
MfeI CAATTG 1 cut(s) 1347
MflI RGATCY 2 cut(s) 97, 1911
MhlI GDGCHC 1 cut(s) 3158
MlyI GAGTC 5 cut(s) 231, 1679, 2882, 3353, 3556
MmeI TCCRAC 4 cut(s) 1212, 1522, 1756, 3436
Mph1103I ATGCAT 2 cut(s) 2844, 2941
MroI TCCGGA 1 cut(s) 1600
MroXI GAANNNNTTC 2 cut(s) 111, 122
MseI TTAA 6 cut(s) 486, 524, 764, 1266, 1995, 2061
MslI CAYNNNNRTG 3 cut(s) 2178, 2453, 3149
MspA1I CMGCKG 2 cut(s) 1483, 2096
MspCI CTTAAG 1 cut(s) 1265
MspI CCGG 4 cut(s) 1601, 3123, 3216, 3457
MspR9I CCNGG 8 cut(s) 258, 1071, 2340, 2540, 2961, 3217, 3368, 3524
MunI CAATTG 1 cut(s) 1347
Mva1269I GAATGC 2 cut(s) 1027, 1397
MvaI CCWGG 7 cut(s) 258, 1071, 2340, 2540, 2961, 3368, 3524
MvnI CGCG 2 cut(s) 969, 2402
MwoI GCNNNNNNNGC 6 cut(s) 980, 2218, 2408, 3317, 3387, 3462
NciI CCSGG 1 cut(s) 3217
NcoI CCATGG 1 cut(s) 628
NlaIV GGNNCC 8 cut(s) 255, 506, 1913, 2192, 3121, 3156, 3447, 3448
NmuCI GTSAC 5 cut(s) 611, 1199, 1689, 2298, 3296
NruI TCGCGA 1 cut(s) 969
NsiI ATGCAT 2 cut(s) 2844, 2941
NspI RCATGY 6 cut(s) 395, 1286, 2128, 2842, 3187, 3398
PaeI GCATGC 2 cut(s) 395, 2842
PagI TCATGA 1 cut(s) 2173
PceI AGGCCT 1 cut(s) 1307
PciI ACATGT 3 cut(s) 2124, 3183, 3394
PcsI WCGNNNNNNNCGW 1 cut(s) 1339
PctI GAATGC 2 cut(s) 1027, 1397
PdmI GAANNNNTTC 2 cut(s) 111, 122
PfeI GAWTC 6 cut(s) 1790, 2170, 2458, 2687, 2994, 3178
Pfl23II CGTACG 1 cut(s) 3501
PinAI ACCGGT 1 cut(s) 3122
PkrI GCNGC 7 cut(s) 8, 139, 476, 1293, 1485, 1518, 2980
Ple19I CGATCG 1 cut(s) 3419
PleI GAGTC 5 cut(s) 230, 1678, 2881, 3353, 3556
PpsI GAGTC 5 cut(s) 230, 1678, 2881, 3353, 3556
PpuMI RGGWCCY 2 cut(s) 2191, 3446
PscI ACATGT 3 cut(s) 2124, 3183, 3394
Psp5II RGGWCCY 2 cut(s) 2191, 3446
Psp6I CCWGG 7 cut(s) 256, 1069, 2338, 2538, 2959, 3366, 3522
PspGI CCWGG 7 cut(s) 256, 1069, 2338, 2538, 2959, 3366, 3522
PspLI CGTACG 1 cut(s) 3501
PspN4I GGNNCC 8 cut(s) 255, 506, 1913, 2192, 3121, 3156, 3447, 3448
PspOMI GGGCCC 1 cut(s) 3154
PspPI GGNCC 5 cut(s) 2191, 3154, 3155, 3218, 3446
PspPPI RGGWCCY 2 cut(s) 2191, 3446
PsrI GAACNNNNNNTAC 2 cut(s) 398, 430
PstI CTGCAG 2 cut(s) 1488, 3475
PstNI CAGNNNCTG 4 cut(s) 724, 2696, 3175, 3529
PsuI RGATCY 2 cut(s) 97, 1911
PvuI CGATCG 1 cut(s) 3419
PvuII CAGCTG 1 cut(s) 2096
RruI TCGCGA 1 cut(s) 969
RsaI GTAC 8 cut(s) 529, 1657, 1887, 2047, 2105, 2387, 2901, 3503
RsaNI GTAC 8 cut(s) 528, 1656, 1886, 2046, 2104, 2386, 2900, 3502
RseI CAYNNNNRTG 3 cut(s) 2178, 2453, 3149
SalI GTCGAC 1 cut(s) 841
SaqAI TTAA 6 cut(s) 486, 524, 764, 1266, 1995, 2061
SatI GCNGC 7 cut(s) 7, 138, 475, 1292, 1484, 1517, 2979
Sau96I GGNCC 5 cut(s) 2191, 3154, 3155, 3218, 3446
ScaI AGTACT 1 cut(s) 2047
SchI GAGTC 5 cut(s) 231, 1679, 2882, 3353, 3556
ScrFI CCNGG 8 cut(s) 258, 1071, 2340, 2540, 2961, 3217, 3368, 3524
SduI GDGCHC 1 cut(s) 3158
SfcI CTRYAG 4 cut(s) 1484, 2041, 2149, 3471
SinI GGWCC 2 cut(s) 2191, 3446
SmiMI CAYNNNNRTG 3 cut(s) 2178, 2453, 3149
SmlI CTYRAG 2 cut(s) 1265, 2877
SmoI CTYRAG 2 cut(s) 1265, 2877
SpeI ACTAGT 1 cut(s) 775
SphI GCATGC 2 cut(s) 395, 2842
SseBI AGGCCT 1 cut(s) 1307
SsiI CCGC 8 cut(s) 137, 1241, 1291, 1481, 1504, 1559, 2551, 3093
SspMI CTAG 5 cut(s) 87, 387, 776, 1361, 2861
StuI AGGCCT 1 cut(s) 1307
StyD4I CCNGG 8 cut(s) 256, 1069, 2338, 2538, 2959, 3215, 3366, 3522
StyI CCWWGG 4 cut(s) 386, 628, 877, 2329
TaiI ACGT 7 cut(s) 603, 1831, 2321, 2479, 2640, 3298, 3498
TaqI TCGA 9 cut(s) 48, 397, 842, 1254, 2010, 2257, 2616, 3048, 3558
TatI WGTACW 4 cut(s) 2045, 2103, 2385, 2899
TauI GCSGC 2 cut(s) 140, 1294
TfiI GAWTC 6 cut(s) 1790, 2170, 2458, 2687, 2994, 3178
Tru1I TTAA 6 cut(s) 486, 524, 764, 1266, 1995, 2061
Tru9I TTAA 6 cut(s) 486, 524, 764, 1266, 1995, 2061
TscAI CASTG 9 cut(s) 448, 931, 1577, 1740, 2274, 2475, 2689, 2728, 3252
TseFI GTSAC 5 cut(s) 611, 1199, 1689, 2298, 3296
TseI GCWGC 5 cut(s) 6, 474, 1483, 1516, 2978
Tsp45I GTSAC 5 cut(s) 611, 1199, 1689, 2298, 3296
TspGWI ACGGA 6 cut(s) 545, 998, 2934, 3330, 3448, 3519
TspRI CASTG 9 cut(s) 448, 931, 1577, 1740, 2274, 2475, 2689, 2728, 3252
Vha464I CTTAAG 1 cut(s) 1265
VpaK11BI GGWCC 2 cut(s) 2191, 3446
XagI CCTNNNNNAGG 1 cut(s) 512
XapI RAATTY 4 cut(s) 121, 519, 1630, 1939
XceI RCATGY 6 cut(s) 395, 1286, 2128, 2842, 3187, 3398
XmaJI CCTAGG 1 cut(s) 386
XmiI GTMKAC 2 cut(s) 842, 3108
XmnI GAANNNNTTC 2 cut(s) 111, 122
XspI CTAG 5 cut(s) 87, 387, 776, 1361, 2861
ZraI GACGTC 1 cut(s) 2319
ZrmI AGTACT 1 cut(s) 2047
Zsp2I ATGCAT 2 cut(s) 2844, 2941
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.