RLG00000034111

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0002206
Physical Location & Seq
Reverse (-)
36879320 .. 36892565
13246 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000034111

Sequence Viewer

Length: 5907 bp
ATGGTCTTAGACGTTTTAGCTCCTCGCGTTGAGTACCAAAAAGACGGAGCAGATCGAGACAGAGAGAGTGATCAACTGCAAAAATCCGAGAAGCCTCTCACGTGCATTTTTTCAAATCCAATTGGGGCTTCATCGGATAGATGTACGGAGGCATCAGTTTCATTCTTCAACCAGCATGGCCTCATTAGTCACCAAATAAACTCGTACAATGACTTCATTAAAAATGGTATACAAAGAGTCGTTGATAGTTATGATGAGATTATAGTTGAGCCTGCGTATGGCCCATCGAAGAAGGGGAATGCAGAATGGCGATATGCTTCTGTCAGGTTTGAGAAGCTTACCCTCATTCGACCAAGTTTTTGGGGCGGTGCTCTTCAAAATATGACATACTCAGCCAAGATCAAAGTGAATGTTACGACTGAGGTATATACTCAAAATCTTGTCAGAAGCGAAAAGTTTAAAACTGGAAAAGAACAATGTCTTGACAAGCAAATTGTGACTACAGAGAGTAGAGATGTCTTTATTGGTAAGCTCCCAGTGATGGTGAAGTCTGACTTGTGTTGGTTGAAAGACGAGAGAGCTGAGTGTGAATTTGACCATGGAGATTATTTTATAATCAAGGGTGTGAGAAGTAATCAGGGGCTCACAGTAGCATATAGGTCTGAGGTCAAGCGGCATAGACTAATAATAAGATTATCTGGGATTTCTCAACTTGAAAACATTGGAGTAGTGAAAGTTTTGTCAGTGTACTTTATGTCAGCAGAAATCCCTGTCTGGATTTGGTTTTTCACCCTTGGGGTTTCTTTGGACAGTGAAGTTATAGATTTGATTGATTATGGTAAAGAAGATGCAAGCATTTCAAACATTCTAAATGTTCTCAGATATGTTGAAGAGATGATAAAGAATACCACGTTTCCTCCTCAGGAAAGCATTGAAGAATGCATCAACATGTATCTGTTCCCCAATCTGAGAGGCATGAAGCAAAAGGCTCAGTCTCTTGGCTATATGGTGAAGTCTCTTTTACAGACCTTCAGAGGCCACAAGAAATGTGACAACAGAGAAGAATTGAAGTACAAGAGGCTTCACTTGGCTGGTGAGCTTCTAGAGGGAGAGCTGAAGGCACACATAGGACATGCAGGGAGGCGCATGGCAAAGGCTTTGCAGGGAGACCTTTGTGGTGACCGTGTTGTCCGCCCGGTTGAGCAATATTTAGATGCTACAATAGTAACTAATGGCATCTCCAGAGCATTCTCAACAGGAGCATGGTGTCATCCTTTCAAGACTATGGAGAGGATATCTGGTGTGGTGTTAAATGTTGGGAAAACAAATCCACCCCAAACAATGGTAGATATGAGGAAGACACGCCTCCATGATCAATACATGGGGAAGGTTGGAGATGCCAGATATCCGCATCCTTCTCACTGGGGTAGGATTTGCTTTATCTCAACCCCAGATGGTGAGAATTGTGGGCTTGTGAAAAATTTGGCTACCACTGCACTGACAAGTACAAACATATTGGAGCCCATATTGCCTGAGTTTTTCTCTTGTGCCATGGAGGAGCTGGTAGATGATACTTCAACATCACTTCATGGGAAGAATAAAGATTTTCTCAATGGGGATTGGGTTGGCGTTTGTGAAGATTCCCATGATTTTGTCATGAAACTAAGGACATTGAGACGCAGGAAAAAGTTGCTACAGCAGGTGGAAATCAAAAGGGATGAGCAGCTCCATGAAGTACGCATATATTCTGATGCTGGAAGGATTCTACGTCCTTTCCTGGTAGTTAAGAATTTGAATAAGATTAAAGCATCCAAGGGAAAAAAACACACGTTCCAAGATCTTCTTGGCAATGGAATAATCGAGCTCATTGGAGCTGAAGAAGAAGAGGATTGCTGTACAGCATGGGACGTCAGGCATCTATTTATGGAAGACAATGGGAAGTCAGCAGTCAAGTACACACACTGTGAACTGGACCTGTCATTTTTGTTAGGCTTAAGTTGTGGTATCATTCCCTTTTCAAACCATGATCATGCAAGGAGGGTTCTATACCAGTCTCAGAAGCACTCTCAGCAGGTCATTGGTTTTTCCACCACAAATCCTAACCACAGAGTGGTTACTCTGTCTCACCAACTACGCTACCCTCAGAGGCCGCTTTTTTGTAATATGATATCTGATTGCCTTGGCCAATCAGGAAACCCAGTGAGCCATGACAGAGTTATGTCCAAGCCTCAATACTTTAATGGCCAAAATGCAATTGTTGCATTCAATGTGCACCTAGGATATAACCAAGAAGATTCTATTGTGATGAACCGAGCATCTGTGCAATGTGGCATGTTCAAATCTGAGCACATATGGAGCTACAAGGCTGAAGCTGAAAACAAGTTCTCATTGGGAAAAAGGAGGAATCCAGATGACTGTATAAACTTTGGCAAAATACAGAGTAGATTTGGAAGTGTTGACAGCCTTGATGATGATGGATTCCCTCGTATAGGTGCATATCTGCTGAGTGGTGATATTATTATTGGGAGGTGCAGTGAATCTGGTACTGATCATAGCATAAAACTGAGGCATACTGAAAGAGGAAATGTTCAGAAGGTTGTACTTTCCTCTAATGACGAGGGAAAGAATTTTGGTGTTGTATCCTTAAGACAGGTTTGTTCTCCTTGTCTTGGAGACAAGTTCTCAAGCATGCATGGGTCTCCTGGTCAATTCCTAGAGGCTGCTTTGGGGAAGGGAATTGCCTGTGGTGGCTCAATGAGATATGCAACTCCTTTCTCTACTCCCTCTGTTGATGACACCACAGATCAGCTTCATGGAGCTGGATTTCAAAGATGGGGAAATGAGAGAGTATACAGTGGTCAAACTGGTCAAATGCCAGTGGCAGACAGGAAGAGATTTTGTGGGGTAAAGTTTGGTGAGATGGAGCTCGACTGCCTTATAGCCCATGGAGCATCAGCAAACTTACATGAACGCCTTTTCACTCTTTGTGACTCCTCCCAAATGCATTCTTGCCAGAAATGCAAAAATGCGGCTAATGTGATAGATGGAACAGTTGATGGCCGTAGAATCAGGGGTCCCTATTGCCTGGTCTGTAAGTCTGTTGATGACATTGTCAGGTTGAATGTTCCATACGGGGCCAAGTTACTGTGCCATGAACTTTTCATTAATGCTATTACGACACTCCAGCATGAGTGGGGCATTAAACCTCCAAGTTGGAATGGTTCAGAGCCTTGTGACAACTGGGAAGGTATTGAATGCACCAATTCCCGGATTACTGCTATAGCATTGGCAGACATGGGTTTGGAAGGTCGGCTATCCAGTGAACTTGAGACGTTCTCTGAGCTAAAGATCCTGGTTCTGTCTTACAACAAGGACCTGACAGGACAACTTCACGAAGCATCAATTGGACGCTTGAGGAAACTGGAAACCTTAATCCTGGATGGTTGCGATTTCTCTGGTCCTATTCCAGCTACAATAGGATCTCTAAAGCAACTCTTTTATCTATCTCTGAAAAACAATAGATTCGGTGGACCAATTCCGCCTTCTATCGGTAATCTGTCCAATCTAGTCTTTCTGGACCTATCAGACAACAAACTTGAGGGTCCCATTCCTGTCTCTAGTAGGACTACATCTGGTCTTGATATGTTAAATAAAACAGAGCACTTTCATTTTTCAAATAACCAGCTTTCAGGCAATATCCCCCCTCAACTCTTCAGCTCAGAGATGGCTCTTAAACATTTGATGTTAGACCGAAATAATCTAGATGGCAGCATCCCATCTAATCTCGGACTGGTGAAAACTCTGCAGGCAATACGCCTTGATAGGAATTCTTTAAGCGGGACTGTGCCATCAAGCCTCAACAATCTTCGGAATGTAGCTGAATTGCACTTGTCCAACAATATGCTGAGTGGCCCGGTGCCAAACCTTACTGGCATGGATTCCCTCTATTATGTGGACATCAGCAATAATTCTTTTGATGCACCAGAGTTTCCGAATTGGTTTTCAATCTTGACGTTTTTGACAACATTAATGATGGAAAACATAGGACTTCAGGGACAAGTTCCTCGAGCCCTTTTCAGCCTTGAATATTTGGAGTCAGTGGTACTAAGAAACAACAATCTCAGTGATTCATTGGATATTGGAACCAATTATAGCAAACAATTGGAGCTAGTTGATCTGCAGAAGAACAACATTGTCGTTCTTGCACAGAACTATGATGGATCCAACTACACGCTCATTCTAGTTGAAAATCCAATTTGTGAGGGAAGCAAGTACAACATGGCAAAGAAATACTGTAATATTTCTCCACCAGATTCCTCAATTTCAACTCCAGGAAGTAACTGTCCTACTCCTGTCTGTAAATCCGGCCAGTTTGCTTCCCCCAACTGTAATTGTGCATTTCCCTACACACAGACTCTAGTCTTCTTATTTGTTTCTTACTCAAACTTGGAAGACATAAACTATTACATTCTTCTCGCGGAAAATCTCACAAGATCTTTACAGTCCCTCAACCTTCCTGTGGATTCAGTTTCTCTGAGTTATCCTAAATTGGACTCATCTTCTCAACTTGAACTGACAATACGAATTTTCCCATCTGGTGGTCAAGTTAGCTTCACCCAAGCAGAAGAGTCAGCTATATCAACTGTTCTTAGCAACCAGACTCTCAATGCACGTCCAAGATATTTTGGACCCTTCTCTTTTCTTGTTCCTTACACCAACTCTGGAGGATCAAACAAGGCCGTGATAATAGGTACAGCAATTGGTGGTTCTGTGCTCGTTTCATTATTAGTCCTTGTAGGGCTTTATGCTCTCCAGCAGAAGAGGAGAGCCGACGAGTCATTGAGTGGATCAATACTTTTGCAGACAGGGATTTCAAACAGCAGTGATAGTGGTCCTCAACTAAAAGGAGCAAGATTGTTCTCATTTGAAGAGCTCAAGAAATGCACCGATGGTTTTTCAGAAGCAAATAATATTGGATCTGGTGGTTATGGCCAGGTATATCGGGGAATTCTTCCTACAGCTCAGATGGTTGCCATTAAAAGAGCGAAAAGGGAATCTATGCAGGGAGGAGTTGAGTTCAAAGCCGAGGTTGAGCTCCTATCACGAGTACATCATAAGAATCTTGTTGGCCTTGTTGGTTTTTGCTTTGAACAAGGAGAACAAATATTGGTATATGAGTATGTTCCAAATGGTGATTTGAGGGACAGCCTATCAGGGAAGTCCGGAATCAGGTTGGACTGGATGAGAAGACTTCAAGTAGCACTTGGTGCGGCGAGAGGTCTGGCATATCTTCATGAACTTGCAAGCCCAACCATTATACACCGGGACATCAAATCAAACAACATTCTGCTCGATAAAGATTTAAATGCAAGAGTTGCTGATTTTGGTCTCTCCAAGTCAATTGCTGATGGTGGAATGGACCACTTATCTACTCAAGTTAAAGGGACACTGGGCTACTTGGATCCTGAATATTATATGACTCAACAGTTGACTGAGAAGAGCGACGTGTATAGCTTTGGCGTGGTAATGTTGGAGCTGATAACTGCTAGAAGGCCAATAGTGCAAGGGAAATATATAGTGAGAGTGGTACAGATGACAATGGATAAGTCAAAAGATTTGTACAACCTTGATGACATTCTTGATCCATTCATTGGTTTAGGAACAGAGCTGAAAGGTGTAGAAATGTTTGTGGATTTGGCAATGGCTTGTGTAGAAGAATTAAGGGATAAAAGACCAAGAATGGGGGAAGTGGTGAAACAGATAGAGAACATTATACAAATCGCTGCTTTCAACGCCGGTACCAATCCATTGCCCACTTTAGCAAGTTATGAGGAGTATGAGAGTATGGACAGCACCAAGGATCTTTACAGCAGACCCTTTGATTATAGTGGAGCTTTTGTCGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000228 GO:0000418 GO:0000419 GO:0000428 GO:0000785 GO:0000790 GO:0000792 GO:0002682 GO:0003674 GO:0003824 GO:0003899 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005720 GO:0005730 GO:0005736 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009605 GO:0009607 GO:0009620 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016020 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019222 GO:0019438 GO:0030422 GO:0030880 GO:0031047 GO:0031050 GO:0031974 GO:0031981 GO:0032259 GO:0032774 GO:0032991 GO:0034062 GO:0034641 GO:0034654 GO:0035194 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043331 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044451 GO:0044452 GO:0044454 GO:0044464 GO:0044728 GO:0046483 GO:0048519 GO:0048583 GO:0050776 GO:0050789 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0055029 GO:0060255 GO:0061695 GO:0065007 GO:0070013 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0097747 GO:0098542 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1901698 GO:1901699 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko01100 ko03020 ko05016 ko05169 map00230 map00240 map01100 map03020 map05016 map05169
Pfam Domains
Protein Families
RNA_pol_Rpb2_1 RNA_pol_Rpb2_2 RNA_pol_Rpb2_3 RNA_pol_Rpb2_4 RNA_pol_Rpb2_5 RNA_pol_Rpb2_6 RNA_pol_Rpb2_7

Protein Analysis

1969

Amino Acids

219.15

Weight (kDa)

6.43

Isoelectric Point (pI)

39.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNA_pol_Rpb2_1 PF04563 60 - 390 1.1e-17 RNA polymerase beta subunit
RNA_pol_Rpb2_3 PF04565 441 - 499 2.8e-15 RNA polymerase Rpb2, domain 3
RNA_pol_Rpb2_4 PF04566 536 - 596 1.4e-20 RNA polymerase Rpb2, domain 4
RNA_pol_Rpb2_5 PF04567 611 - 658 6.5e-08 RNA polymerase Rpb2, domain 5
RNA_pol_Rpb2_6 PF00562 667 - 900 8.4e-39 RNA polymerase Rpb2, domain 6
RNA_pol_Rpb2_6 PF00562 902 - 958 2.5e-07 RNA polymerase Rpb2, domain 6
RNA_pol_Rpb2_7 PF04560 968 - 1054 1.4e-18 RNA polymerase Rpb2, domain 7
LRR_14 PF23598 1107 - 1198 6.2e-07 Leucine-rich repeat region
Pkinase PF00069 1653 - 1919 1.6e-42 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 1656 - 1919 3.9e-45 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 614
AarI CACCTGC 1 cut(s) 1690
AasI GACNNNNNNGTC 1 cut(s) 1187
AatII GACGTC 1 cut(s) 1911
Acc36I ACCTGC 2 cut(s) 1690, 2062
Acc65I GGTACC 1 cut(s) 5798
AccB1I GGYRCC 2 cut(s) 3916, 5798
AccB7I CCANNNNNTGG 4 cut(s) 1342, 2110, 4598, 5651
AccI GTMKAC 2 cut(s) 229, 2850
AccII CGCG 2 cut(s) 27, 4478
AccIII TCCGGA 1 cut(s) 5222
AcoI YGGCCR 5 cut(s) 2182, 2242, 3058, 4366, 4990
AcsI RAATTY 7 cut(s) 590, 1480, 1789, 2626, 3826, 4584, 5007
AcuI CTGAAG 6 cut(s) 1015, 1136, 1896, 2388, 3697, 4034
AcvI CACGTG 1 cut(s) 102
AcyI GRCGYC 1 cut(s) 1908
AdeI CACNNNGTG 3 cut(s) 1964, 2110, 5267
AflII CTTAAG 2 cut(s) 1993, 2644
AflIII ACRYGT 3 cut(s) 948, 1827, 5505
AjiI CACGTC 2 cut(s) 4673, 5506
AjnI CCWGG 7 cut(s) 1776, 2701, 3084, 3351, 3435, 4330, 4992
AjuI GAANNNNNNNTTGG 6 cut(s) 112, 144, 3387, 3419, 3526, 3558
Alw21I GWGCWC 9 cut(s) 373, 1866, 2274, 2349, 2928, 3663, 4776, 4935, 5097
Alw44I GTGCAC 1 cut(s) 2270
Ama87I CYCGRG 1 cut(s) 4065
Aor13HI TCCGGA 1 cut(s) 5222
ApaLI GTGCAC 1 cut(s) 2270
ApeKI GCWGC 4 cut(s) 1723, 2720, 3768, 5783
ApoI RAATTY 7 cut(s) 590, 1480, 1789, 2626, 3826, 4584, 5007
ArsI GACNNNNNNTTYG 2 cut(s) 342, 374
AseI ATTAAT 2 cut(s) 3165, 4028
Asp718I GGTACC 1 cut(s) 5798
AspA2I CCTAGG 1 cut(s) 2275
AspLEI GCGC 1 cut(s) 1146
AsuC2I CCSGG 4 cut(s) 1196, 3268, 3914, 5324
AvaI CYCGRG 1 cut(s) 4065
AvrII CCTAGG 1 cut(s) 2275
AxyI CCTNAGG 1 cut(s) 921
BaeGI GKGCMC 1 cut(s) 2274
BaeI ACNNNNGTAYC 2 cut(s) 5782, 5815
BalI TGGCCA 3 cut(s) 2184, 2244, 4992
BamHI GGATCC 2 cut(s) 4220, 5461
BanI GGYRCC 2 cut(s) 3916, 5798
BanII GRGCYC 7 cut(s) 645, 1524, 1866, 2928, 4072, 4935, 5097
BarI GAAGNNNNNNTAC 2 cut(s) 197, 229
BauI CACGAG 1 cut(s) 5103
BbrPI CACGTG 1 cut(s) 102
BbsI GAAGAC 5 cut(s) 1364, 1935, 4414, 4458, 5254
Bbv12I GWGCWC 9 cut(s) 373, 1866, 2274, 2349, 2928, 3663, 4776, 4935, 5097
BbvI GCAGC 4 cut(s) 1735, 2707, 3780, 5770
BceAI ACGGC 2 cut(s) 3045, 4724
BciT130I CCWGG 7 cut(s) 1778, 2703, 3086, 3353, 3437, 4332, 4994
BciVI GTATCC 1 cut(s) 2650
BclI TGATCA 4 cut(s) 70, 1372, 2026, 2548
BcnI CCSGG 4 cut(s) 1196, 3268, 3914, 5324
BfmI CTRYAG 6 cut(s) 501, 1694, 3279, 3803, 4178, 5016
BfrI CTTAAG 2 cut(s) 1993, 2644
BfuAI ACCTGC 2 cut(s) 1690, 2062
BfuI GTATCC 1 cut(s) 2650
BglII AGATCT 2 cut(s) 1837, 4493
BisI GCNGC 8 cut(s) 674, 1724, 2150, 2721, 3030, 3769, 5271, 5784
BlnI CCTAGG 1 cut(s) 2275
BlsI GCNGC 8 cut(s) 675, 1725, 2151, 2722, 3031, 3770, 5272, 5785
BmeT110I CYCGRG 1 cut(s) 4065
BmgBI CACGTC 2 cut(s) 4673, 5506
BmrI ACTGGG 5 cut(s) 530, 1432, 2192, 3250, 5459
BmuI ACTGGG 5 cut(s) 530, 1432, 2192, 3250, 5459
BoxI GACNNNNGTC 1 cut(s) 4418
BpiI GAAGAC 5 cut(s) 1364, 1935, 4414, 4458, 5254
BplI GAGNNNNNCTC 4 cut(s) 1526, 1558, 3320, 3352
BpmI CTGGAG 5 cut(s) 1225, 3167, 4314, 4743, 4796
BpuEI CTTGAG 6 cut(s) 2668, 3347, 3433, 3617, 4919, 5418
BpuMI CCSGG 4 cut(s) 1196, 3268, 3914, 5324
BsaAI YACGTR 1 cut(s) 102
BsaHI GRCGYC 1 cut(s) 1908
BsaI GGTCTC 3 cut(s) 1161, 2703, 5393
BsaJI CCNNGG 9 cut(s) 598, 793, 1551, 1812, 2179, 2275, 2944, 5085, 5856
BsaWI WCCGGW 1 cut(s) 5222
BsaXI ACNNNNNCTCC 5 cut(s) 901, 931, 2518, 2548, 5883
Bse118I RCCGGY 1 cut(s) 5795
Bse21I CCTNAGG 1 cut(s) 921
Bse3DI GCAATG 4 cut(s) 1855, 2330, 5706, 5807
BseAI TCCGGA 1 cut(s) 5222
BseBI CCWGG 7 cut(s) 1778, 2703, 3086, 3353, 3437, 4332, 4994
BseDI CCNNGG 9 cut(s) 598, 793, 1551, 1812, 2179, 2275, 2944, 5085, 5856
BseGI GGATG 7 cut(s) 1270, 1411, 1723, 1808, 3445, 3771, 5247
BseMI GCAATG 4 cut(s) 1855, 2330, 5706, 5807
BseRI GAGGAG 7 cut(s) 12, 909, 1571, 2983, 4837, 5082, 5846
BseSI GKGCMC 1 cut(s) 2274
BseXI GCAGC 4 cut(s) 1735, 2707, 3780, 5770
BsgI GTGCAG 2 cut(s) 1479, 2551
Bsh1236I CGCG 2 cut(s) 27, 4478
BshNI GGYRCC 2 cut(s) 3916, 5798
BsiHKAI GWGCWC 9 cut(s) 373, 1866, 2274, 2349, 2928, 3663, 4776, 4935, 5097
BsiHKCI CYCGRG 1 cut(s) 4065
BsiSI CCGG 7 cut(s) 1196, 3268, 3914, 4365, 5223, 5323, 5796
BslFI GGGAC 9 cut(s) 1919, 3060, 3588, 3853, 4068, 4489, 5216, 5339, 5458
BsmBI CGTCTC 2 cut(s) 1669, 3323
BsmFI GGGAC 9 cut(s) 1919, 3060, 3588, 3853, 4068, 4489, 5216, 5339, 5458
BsmI GAATGC 6 cut(s) 304, 944, 1247, 2261, 3004, 3260
Bso31I GGTCTC 3 cut(s) 1161, 2703, 5393
BsoBI CYCGRG 1 cut(s) 4065
Bsp13I TCCGGA 1 cut(s) 5222
Bsp1407I TGTACA 2 cut(s) 1895, 5619
Bsp19I CCATGG 3 cut(s) 598, 1551, 2944
BspEI TCCGGA 1 cut(s) 5222
BspFNI CGCG 2 cut(s) 27, 4478
BspHI TCATGA 2 cut(s) 1656, 5293
BspMAI CTGCAG 2 cut(s) 3807, 4182
BspMI ACCTGC 2 cut(s) 1690, 2062
BspQI GCTCTTC 3 cut(s) 378, 4923, 5492
BspT107I GGYRCC 2 cut(s) 3916, 5798
BspTI CTTAAG 2 cut(s) 1993, 2644
BspTNI GGTCTC 3 cut(s) 1161, 2703, 5393
BsrDI GCAATG 4 cut(s) 1855, 2330, 5706, 5807
BsrFI RCCGGY 1 cut(s) 5795
BsrGI TGTACA 2 cut(s) 1895, 5619
BssAI RCCGGY 1 cut(s) 5795
BssECI CCNNGG 9 cut(s) 598, 793, 1551, 1812, 2179, 2275, 2944, 5085, 5856
BssNAI GTATAC 2 cut(s) 230, 2851
BssNI GRCGYC 1 cut(s) 1908
BssSI CACGAG 1 cut(s) 5103
BssT1I CCWWGG 8 cut(s) 598, 793, 1551, 1812, 2179, 2275, 2944, 5856
Bst1107I GTATAC 2 cut(s) 230, 2851
Bst2BI CACGAG 1 cut(s) 5103
Bst2UI CCWGG 7 cut(s) 1778, 2703, 3086, 3353, 3437, 4332, 4994
Bst6I CTCTTC 9 cut(s) 378, 885, 1878, 2885, 3716, 4620, 4814, 4923, 5492
BstACI GRCGYC 1 cut(s) 1908
BstAFI CTTAAG 2 cut(s) 1993, 2644
BstAPI GCANNNNNTGC 3 cut(s) 2258, 5267, 5375
BstAUI TGTACA 2 cut(s) 1895, 5619
BstBAI YACGTR 1 cut(s) 102
BstC8I GCNNGC 5 cut(s) 273, 853, 2690, 3807, 5305
BstDSI CCRYGG 3 cut(s) 598, 1551, 2944
BstEII GGTNACC 1 cut(s) 1178
BstENI CCTNNNNNAGG 1 cut(s) 2487
BstF5I GGATG 7 cut(s) 1270, 1411, 1723, 1808, 3445, 3771, 5247
BstFNI CGCG 2 cut(s) 27, 4478
BstHHI GCGC 1 cut(s) 1146
BstNI CCWGG 7 cut(s) 1778, 2703, 3086, 3353, 3437, 4332, 4994
BstNSI RCATGY 4 cut(s) 952, 1136, 2335, 2692
BstPAI GACNNNNGTC 1 cut(s) 4418
BstPI GGTNACC 1 cut(s) 1178
BstSFI CTRYAG 6 cut(s) 501, 1694, 3279, 3803, 4178, 5016
BstSLI GKGCMC 1 cut(s) 2274
BstUI CGCG 2 cut(s) 27, 4478
BstV1I GCAGC 4 cut(s) 1735, 2707, 3780, 5770
BstV2I GAAGAC 5 cut(s) 1364, 1935, 4414, 4458, 5254
BstX2I RGATCY 8 cut(s) 1837, 3348, 3479, 4220, 4493, 4976, 5461, 5860
BstXI CCANNNNNNTGG 1 cut(s) 360
BstYI RGATCY 8 cut(s) 1837, 3348, 3479, 4220, 4493, 4976, 5461, 5860
BstZ17I GTATAC 2 cut(s) 230, 2851
Bsu36I CCTNAGG 1 cut(s) 921
BsuI GTATCC 1 cut(s) 2650
BtgI CCRYGG 3 cut(s) 598, 1551, 2944
BtrI CACGTC 2 cut(s) 4673, 5506
BtsCI GGATG 7 cut(s) 1270, 1411, 1723, 1808, 3445, 3771, 5247
BtsI GCAGTG 3 cut(s) 1491, 2539, 4888
BveI ACCTGC 2 cut(s) 1690, 2062
Cac8I GCNNGC 5 cut(s) 273, 853, 2690, 3807, 5305
CciI TCATGA 2 cut(s) 1656, 5293
CfoI GCGC 1 cut(s) 1146
Cfr10I RCCGGY 1 cut(s) 5795
CseI GACGC 2 cut(s) 1686, 3417
DraI TTTAAA 2 cut(s) 460, 5364
DraIII CACNNNGTG 3 cut(s) 1964, 2110, 5267
DrdI GACNNNNNNGTC 1 cut(s) 1187
DseDI GACNNNNNNGTC 1 cut(s) 1187
EaeI YGGCCR 5 cut(s) 2182, 2242, 3058, 4366, 4990
Eam1104I CTCTTC 9 cut(s) 378, 885, 1878, 2885, 3716, 4620, 4814, 4923, 5492
EarI CTCTTC 9 cut(s) 378, 885, 1878, 2885, 3716, 4620, 4814, 4923, 5492
EciI GGCGGA 2 cut(s) 1181, 3528
Ecl136II GAGCTC 4 cut(s) 1864, 2926, 4933, 5095
Eco130I CCWWGG 8 cut(s) 598, 793, 1551, 1812, 2179, 2275, 2944, 5856
Eco24I GRGCYC 7 cut(s) 645, 1524, 1866, 2928, 4072, 4935, 5097
Eco31I GGTCTC 3 cut(s) 1161, 2703, 5393
Eco32I GATATC 3 cut(s) 1296, 1406, 2169
Eco53kI GAGCTC 4 cut(s) 1864, 2926, 4933, 5095
Eco57I CTGAAG 6 cut(s) 1015, 1136, 1896, 2388, 3697, 4034
Eco72I CACGTG 1 cut(s) 102
Eco81I CCTNAGG 1 cut(s) 921
Eco88I CYCGRG 1 cut(s) 4065
Eco91I GGTNACC 1 cut(s) 1178
EcoICRI GAGCTC 4 cut(s) 1864, 2926, 4933, 5095
EcoNI CCTNNNNNAGG 1 cut(s) 2487
EcoO109I RGGNCCY 3 cut(s) 3074, 3373, 3602
EcoO65I GGTNACC 1 cut(s) 1178
EcoRI GAATTC 2 cut(s) 3826, 5007
EcoRII CCWGG 7 cut(s) 1776, 2701, 3084, 3351, 3435, 4330, 4992
EcoRV GATATC 3 cut(s) 1296, 1406, 2169
EcoT14I CCWWGG 8 cut(s) 598, 793, 1551, 1812, 2179, 2275, 2944, 5856
EcoT22I ATGCAT 3 cut(s) 944, 2694, 3006
EcoT38I GRGCYC 7 cut(s) 645, 1524, 1866, 2928, 4072, 4935, 5097
ErhI CCWWGG 8 cut(s) 598, 793, 1551, 1812, 2179, 2275, 2944, 5856
Esp3I CGTCTC 2 cut(s) 1669, 3323
FalI AAGNNNNNCTT 8 cut(s) 539, 571, 1827, 1859, 3479, 3511, 5247, 5279
FaqI GGGAC 9 cut(s) 1919, 3060, 3588, 3853, 4068, 4489, 5216, 5339, 5458
FauI CCCGC 1 cut(s) 3830
FauNDI CATATG 1 cut(s) 2351
FbaI TGATCA 4 cut(s) 70, 1372, 2026, 2548
FblI GTMKAC 2 cut(s) 229, 2850
Fnu4HI GCNGC 8 cut(s) 674, 1724, 2150, 2721, 3030, 3769, 5271, 5784
FokI GGATG 7 cut(s) 1257, 1398, 1730, 1795, 3452, 3758, 5254
FriOI GRGCYC 7 cut(s) 645, 1524, 1866, 2928, 4072, 4935, 5097
Fsp4HI GCNGC 8 cut(s) 674, 1724, 2150, 2721, 3030, 3769, 5271, 5784
GlaI GCGC 1 cut(s) 1145
GluI GCNGC 8 cut(s) 674, 1724, 2150, 2721, 3030, 3769, 5271, 5784
GsuI CTGGAG 5 cut(s) 1225, 3167, 4314, 4743, 4796
HapII CCGG 7 cut(s) 1196, 3268, 3914, 4365, 5223, 5323, 5796
HgaI GACGC 2 cut(s) 1686, 3417
HhaI GCGC 1 cut(s) 1146
Hin1I GRCGYC 1 cut(s) 1908
Hin6I GCGC 1 cut(s) 1144
HinP1I GCGC 1 cut(s) 1144
HincII GTYRAC 2 cut(s) 2458, 5490
HindII GTYRAC 2 cut(s) 2458, 5490
HindIII AAGCTT 1 cut(s) 335
HpaII CCGG 7 cut(s) 1196, 3268, 3914, 4365, 5223, 5323, 5796
Hpy99I CGWCG 2 cut(s) 4835, 5507
Hsp92I GRCGYC 1 cut(s) 1908
HspAI GCGC 1 cut(s) 1144
KflI GGGWCCC 2 cut(s) 3074, 3602
Kpn2I TCCGGA 1 cut(s) 5222
KpnI GGTACC 1 cut(s) 5802
Ksp22I TGATCA 4 cut(s) 70, 1372, 2026, 2548
LguI GCTCTTC 3 cut(s) 378, 4923, 5492
Lsp1109I GCAGC 4 cut(s) 1735, 2707, 3780, 5770
MfeI CAATTG 6 cut(s) 120, 2253, 3402, 4160, 4758, 5400
MflI RGATCY 8 cut(s) 1837, 3348, 3479, 4220, 4493, 4976, 5461, 5860
MlsI TGGCCA 3 cut(s) 2184, 2244, 4992
MluNI TGGCCA 3 cut(s) 2184, 2244, 4992
MlyI GAGTC 9 cut(s) 246, 2984, 4103, 4408, 4547, 4637, 4654, 4844, 5473
MmeI TCCRAC 7 cut(s) 1372, 3194, 3918, 4248, 5214, 5511, 5881
Mox20I TGGCCA 3 cut(s) 2184, 2244, 4992
Mph1103I ATGCAT 3 cut(s) 944, 2694, 3006
MroI TCCGGA 1 cut(s) 5222
MscI TGGCCA 3 cut(s) 2184, 2244, 4992
MslI CAYNNNNRTG 2 cut(s) 947, 2028
Msp20I TGGCCA 3 cut(s) 2184, 2244, 4992
MspCI CTTAAG 2 cut(s) 1993, 2644
MspI CCGG 7 cut(s) 1196, 3268, 3914, 4365, 5223, 5323, 5796
MunI CAATTG 6 cut(s) 120, 2253, 3402, 4160, 4758, 5400
Mva1269I GAATGC 6 cut(s) 304, 944, 1247, 2261, 3004, 3260
MvaI CCWGG 7 cut(s) 1778, 2703, 3086, 3353, 3437, 4332, 4994
MvnI CGCG 2 cut(s) 27, 4478
NciI CCSGG 4 cut(s) 1196, 3268, 3914, 5324
NcoI CCATGG 3 cut(s) 598, 1551, 2944
NdeI CATATG 1 cut(s) 2351
NmeAIII GCCGAG 1 cut(s) 5110
NmuCI GTSAC 6 cut(s) 188, 496, 1049, 1178, 2987, 3233
NsiI ATGCAT 3 cut(s) 944, 2694, 3006
NspI RCATGY 4 cut(s) 952, 1136, 2335, 2692
PaeI GCATGC 1 cut(s) 2692
PaeR7I CTCGAG 1 cut(s) 4065
PagI TCATGA 2 cut(s) 1656, 5293
PaqCI CACCTGC 1 cut(s) 1690
PciI ACATGT 1 cut(s) 948
PciSI GCTCTTC 3 cut(s) 378, 4923, 5492
PctI GAATGC 6 cut(s) 304, 944, 1247, 2261, 3004, 3260
PflFI GACNNNGTC 1 cut(s) 3110
PflMI CCANNNNNTGG 4 cut(s) 1342, 2110, 4598, 5651
PfoI TCCNGGA 3 cut(s) 3266, 3435, 4330
PkrI GCNGC 8 cut(s) 675, 1725, 2151, 2722, 3031, 3770, 5272, 5785
PleI GAGTC 9 cut(s) 245, 2984, 4102, 4408, 4547, 4636, 4654, 4843, 5473
PmaCI CACGTG 1 cut(s) 102
PmlI CACGTG 1 cut(s) 102
PpsI GAGTC 9 cut(s) 245, 2984, 4102, 4408, 4547, 4636, 4654, 4843, 5473
Ppu21I YACGTR 1 cut(s) 102
PpuMI RGGWCCY 3 cut(s) 3074, 3373, 3602
PscI ACATGT 1 cut(s) 948
PshAI GACNNNNGTC 1 cut(s) 4418
PshBI ATTAAT 2 cut(s) 3165, 4028
PsiI TTATAA 1 cut(s) 614
Psp124BI GAGCTC 4 cut(s) 1866, 2928, 4935, 5097
Psp5II RGGWCCY 3 cut(s) 3074, 3373, 3602
Psp6I CCWGG 7 cut(s) 1776, 2701, 3084, 3351, 3435, 4330, 4992
PspCI CACGTG 1 cut(s) 102
PspEI GGTNACC 1 cut(s) 1178
PspGI CCWGG 7 cut(s) 1776, 2701, 3084, 3351, 3435, 4330, 4992
PspPPI RGGWCCY 3 cut(s) 3074, 3373, 3602
PspXI VCTCGAGB 1 cut(s) 4065
PsrI GAACNNNNNNTAC 2 cut(s) 4563, 4595
PstI CTGCAG 2 cut(s) 3807, 4182
PsuI RGATCY 8 cut(s) 1837, 3348, 3479, 4220, 4493, 4976, 5461, 5860
PsyI GACNNNGTC 1 cut(s) 3110
RseI CAYNNNNRTG 2 cut(s) 947, 2028
SacI GAGCTC 4 cut(s) 1866, 2928, 4935, 5097
SapI GCTCTTC 3 cut(s) 378, 4923, 5492
SatI GCNGC 8 cut(s) 674, 1724, 2150, 2721, 3030, 3769, 5271, 5784
SchI GAGTC 9 cut(s) 246, 2984, 4103, 4408, 4547, 4637, 4654, 4844, 5473
SfcI CTRYAG 6 cut(s) 501, 1694, 3279, 3803, 4178, 5016
Sfr274I CTCGAG 1 cut(s) 4065
SlaI CTCGAG 1 cut(s) 4065
SmiI ATTTAAAT 1 cut(s) 5364
SmiMI CAYNNNNRTG 2 cut(s) 947, 2028
SmlI CTYRAG 9 cut(s) 1993, 2644, 2683, 3326, 3412, 3596, 4065, 4934, 5433
SmoI CTYRAG 9 cut(s) 1993, 2644, 2683, 3326, 3412, 3596, 4065, 4934, 5433
SphI GCATGC 1 cut(s) 2692
SspI AATATT 6 cut(s) 1208, 4088, 4300, 4972, 5166, 5471
SstI GAGCTC 4 cut(s) 1866, 2928, 4935, 5097
StyI CCWWGG 8 cut(s) 598, 793, 1551, 1812, 2179, 2275, 2944, 5856
SwaI ATTTAAAT 1 cut(s) 5364
TaqI TCGA 7 cut(s) 55, 287, 349, 1860, 2928, 4066, 5352
TaqII GACCGA 1 cut(s) 3765
TatI WGTACW 9 cut(s) 747, 1071, 1505, 1895, 1953, 2599, 4272, 5107, 5619
TauI GCSGC 4 cut(s) 676, 2152, 3032, 5273
TseFI GTSAC 6 cut(s) 188, 496, 1049, 1178, 2987, 3233
TseI GCWGC 4 cut(s) 1723, 2720, 3768, 5783
Tsp45I GTSAC 6 cut(s) 188, 496, 1049, 1178, 2987, 3233
TspGWI ACGGA 2 cut(s) 60, 161
Tth111I GACNNNGTC 1 cut(s) 3110
Van91I CCANNNNNTGG 4 cut(s) 1342, 2110, 4598, 5651
Vha464I CTTAAG 2 cut(s) 1993, 2644
VneI GTGCAC 1 cut(s) 2270
VspI ATTAAT 2 cut(s) 3165, 4028
XagI CCTNNNNNAGG 1 cut(s) 2487
XapI RAATTY 7 cut(s) 590, 1480, 1789, 2626, 3826, 4584, 5007
XbaI TCTAGA 2 cut(s) 1102, 3760
XceI RCATGY 4 cut(s) 952, 1136, 2335, 2692
XcmI CCANNNNNNNNNTGG 2 cut(s) 1558, 1841
XhoI CTCGAG 1 cut(s) 4065
XmaJI CCTAGG 1 cut(s) 2275
XmiI GTMKAC 2 cut(s) 229, 2850
ZraI GACGTC 1 cut(s) 1909
Zsp2I ATGCAT 3 cut(s) 944, 2694, 3006
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.