MD15G1040100.v1.1

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0002206
Physical Location & Seq
Forward (+)
2831010 .. 2837692
6683 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1040100.v1.1.491

Sequence Viewer

Length: 3576 bp
ATGAGCAGCAAGGGAGAAAGTTTTACAAATGGTGTTGACATGGACATCGACGATAGTGACTATGATGATGAACACGATTACTCGACTAGCCTTAACGACCTGGGGGAAGGACTTCTGAAGAATTTCTGCAAGGAGGCAGCAACATCATTCTTCAACGAGTATGGCCTCATTAGTCATCAAATCAACTCATACAACGACTTCATAAAAAATGGCATACAAAGGGTCTTCAGTTCTTTTGGTGAGATTGTAGTTGAGCCTGGCTATGACCCGTCGAAGAAGGGAGATAACGAATGGCGCTATGCTTTAGTGAAGTTTGGGACTGTTACCCTTGGCAGGCCAAGTTTTTGGGGTGGTTCTGACAATGATAAGGAGTATAATATGTTGCCTAGGCATGCTCGACTTCAGAACATGACGTACTGCTCTAAGATGAAAGTGAATATCACCGTCGAGGTATATAATCAACGACTTGTCAGTAGCGATAAGTTTAAGACTGGAAAAGAACGATTTCTTGACAAGGAAATTTTAAGTACAGATACCAGAGACATTACAGTTGGTAGTGTCCCTGTGATGGTGAAGTCCGACTTATGCTGGATGAAGGACGTTGAGAAAGGTGACTGTGATTTTGATCATGGAGGCTATTTTATAATCAAGGGTGCAGAGAAGACCTTTATTGCACAAGAACAGAGTTGTCTGAAGAAACTTCTCATCACAAATAATCAGGGTCTGACGGTAGCATATAGGTCAGAGGTGAAGAGGCATAGGTTAATAATTAGACTAGTCGGAATTTCTAAACTTGAAAACATTGAAGGAGTAGAGAAAGTCCTTACCGTTTACTTCATGTCGACAGAAATCCCTGTGTGGGTGTGGTTTTTTGCGCTTGGCGTTTCATCGGATAGAGAAGTTATTGATCTGATTGATTATGGTAGTGAAGATGCCAGCATTTTGAACATACTTTTTGCCTCAATTCGCGATGCCGATAAAGCTACTGAAAAAGTGGATGGGGGCTTCCGTAGGGGGAAGAATGCCCTCAAATATGTGGATGATGTGATAAAGAAAACTGCATTTCCACCTGGGGAAAGCATCGAAGAGTGCATTAGCTTGTATCTTTTTCCCAATCTCAGAGGCCTAAAGCAAAAGGCTCGCTTTCTTGGGTATATGGTGAAGTCCCTGTTACAAGCCTCCGCTGGTCGCAGGAAATGCGACAATAGGGATGACTTTAGGAACAAGAGGTTGGACTTGGCAGGTGAGCTTCTCGAACGAGAGCTAAAGGCGCATATTGGACATGCAAGGCGGCGCATGGCAAAGGCCTTGCAGAGAGACCTCTACGGTGATCGGGTTGTGCGGCCAATTGAACACTACCTAGATGCCTCCATAGTTACTAATGGCATCTCGAGAGCATTCTCAACTGGAGCATGGTGTCATCCCTTCAAGAGAATGGAGAGGATGTCTGGTGTAGTGGCTAATGTTGGGCGAGCGAATCCGCTGCAGACAATGATTGATATGCGGAAGACAAGGCAGCAGGTTCAATATACAGGGAAGGTTGGAGATGCAAGATACCCGCATCCTTCTCACTGGGGGAAAGTTTGCTTTCTCTCAACCCCAGATGGTGAGAATTGTGGGCTTGTAAAAAATTTGGCCACCACTGGATTAGTAAGTACGAATGTATTGGAATCTCTACTACCTGAATTGTCTCTCTGTGGAATGGAGAAATTGGTGGATGATACTTCTACCTCACTGCAAGGGAAGCTCAAAGTTTTTCTGAATGGGGACTGGGTTGGAGTTTGTGAAGATTCCCTCTCATTTGTCTCGGAACTCAGGAGGATGCGACGTAGGAAAAAGTTGCCACCTCAGGTGGAAATCAAAAGAGATGAAAAGCAAGGAGAAGTACGAGTATATTCTGATGCTGGAAGGATCCTACGCCCTCTCTTGGTAGTTGAGAATTTGAACAAGATAAAAGCATTTAAAGGGGGAAAGTATCCGTTCCAGTATTTTCTTGACAAAGGAATAATCGATCTTATTGGAGCTGAAGAAGAGGAAGACTGTAGTACTGCATGGGGCATTAAATATCTGTTTATGGAAGAAAAGGGAAAATCAGCTGTAAAGTACACACACTGTGAGCTGGACATGTCATTCCTGTTGGGTTTAAGCTGTAGCATTATCCCATTTGCGAATCATGACCATGCAAGGAGGGTCCTCTACCAGGCCCAGAAGCACTCGGCGCAGGCTATTGGGTTTTCTACCACAAATCCTAACCTTCGAGTGGATACGCTGTCTCACCAACTGCACTATCCCCAGAGGCCACTTTTTCAGACAATGACGTCGGACTGCCTTGGCAAACCAGGACACCAACTGGGTCAAAACAGAGTTTTGCCAAAGCCCGAATTGCACAATGGTCAGAATGCGATTGTGGCAGTCAATGTTCACTTGGGATTCAACCAAGAGGATAGCATAGTAATGAACCGGGCATCATTGGAACGTGGTATGTTCCGATCTGAGCATATCAGAAGTTACAAGGCTGAGGTTGACAACAAGGACTCCCTGGAAAAAAGGCGGAAGCCTGATGATTGTGTCAATTTTGGCAAGATGCAGAGCAAGTTTGGCCGTGTTGACAATCTCGATGAGGACGGCTTTCCATATGTCGGTGCAAACTTGCAGAGTGGTGATATCATCATCGGGAGGTGCAGTGAATCAGGAGCTGATCATAGCATCAAACTGAAGCACACCGAAAGGGGAATGGTTCAGAAGGTTGTGCTTTCTTCTAATGATGACGGAAAGAACTTTGCTGTTGTTTCAATGAGACAGGTTCGCTCACCGTGTCTTGGAGACAAGTTCTCGAGCATGCATGGGCAAAAGGGGGTTCTAGGTTTTCTGGAGTCCCAAGAGAACTTCCCTTTCACAGTACAGGGGATAGTTCCAGATATTGTTATAAATCCCCATGCATTTCCGTCAAGACAAACTCCTGGTCAACTCTTAGAAGCTGCTTTAGGGAAGGGAATCGCCTCTGGTGGTCAGAAGAGATATGCCACCCCGTTCTCTACTCTCTCTGTCGATGACATCGCAAAACAACTGCATAGGGCTGGATTTTCAAGATGCGGAAAAGAGAGAGTATACAGTGGTGGCACCGGTCAGATGGTTCGTTCCCTCATATTCATGGGCCCCACCTTTTATCAGCGTCTGATTCACATGTCTGAAGACAAAGTAAAGTTCCGGAACACTGGGCCAGTCCACCCTCTCACCCGTCAGCCAGTGGCAGATCGGAAGCGATTTGGAGGGATCAAGTTTGGAGAAATGGAACGTGACTGCCTAATAGCACACGGAGCTTCAGCAAACTTACATGAGCGCCTCTTCACTCTCAGTGACTCCTCCCAGGTGTATATCTGCCAGAAATGCGAACACGTAGCTAATGTGATCCAACGGTCAGTTGAAGGTGGACGTAAGATCAGGGGTCCGTACTGCCGGAATTGCAATTCTGCAGATGATCTTGTGAAGGCAAACGTCCCGTATGGAGCGAAGTTACTGTGCCAGGAGCTGTTCAGCATGGGGATCAGTTTGAAATACGAGACCCGGTTTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000228 GO:0000418 GO:0000419 GO:0000428 GO:0000785 GO:0000790 GO:0000792 GO:0002682 GO:0003674 GO:0003824 GO:0003899 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005720 GO:0005730 GO:0005736 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009605 GO:0009607 GO:0009620 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016020 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019222 GO:0019438 GO:0030422 GO:0030880 GO:0031047 GO:0031050 GO:0031974 GO:0031981 GO:0032259 GO:0032774 GO:0032991 GO:0034062 GO:0034641 GO:0034654 GO:0035194 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043331 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044451 GO:0044452 GO:0044454 GO:0044464 GO:0044728 GO:0046483 GO:0048519 GO:0048583 GO:0050776 GO:0050789 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0055029 GO:0060255 GO:0061695 GO:0065007 GO:0070013 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0097747 GO:0098542 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1901698 GO:1901699 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko01100 ko03020 ko05016 ko05169 map00230 map00240 map01100 map03020 map05016 map05169
Pfam Domains
Protein Families
RNA_pol_Rpb2_1 RNA_pol_Rpb2_2 RNA_pol_Rpb2_3 RNA_pol_Rpb2_4 RNA_pol_Rpb2_5 RNA_pol_Rpb2_6 RNA_pol_Rpb2_7

Protein Analysis

1192

Amino Acids

134.0

Weight (kDa)

8.85

Isoelectric Point (pI)

35.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNA_pol_Rpb2_1 PF04563 55 - 443 9.7e-32 RNA polymerase beta subunit
RNA_pol_Rpb2_2 PF04561 267 - 412 3.5e-12 RNA polymerase Rpb2, domain 2
RNA_pol_Rpb2_3 PF04565 491 - 548 2.2e-14 RNA polymerase Rpb2, domain 3
RNA_pol_Rpb2_4 PF04566 585 - 646 1.9e-23 RNA polymerase Rpb2, domain 4
RNA_pol_Rpb2_6 PF00562 717 - 1088 9.4e-110 RNA polymerase Rpb2, domain 6
RNA_pol_Rpb2_7 PF04560 1090 - 1187 8.9e-27 RNA polymerase Rpb2, domain 7
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 644, 2927
AarI CACCTGC 1 cut(s) 1232
AasI GACNNNNNNGTC 1 cut(s) 2317
AatII GACGTC 1 cut(s) 2321
Acc36I ACCTGC 2 cut(s) 1232, 1510
AccB1I GGYRCC 1 cut(s) 3119
AccI GTMKAC 2 cut(s) 842, 3108
AccII CGCG 1 cut(s) 969
AccIII TCCGGA 1 cut(s) 3207
AciI CCGC 8 cut(s) 1182, 1291, 1342, 1481, 1504, 1559, 2551, 3093
AclWI GGATC 5 cut(s) 1906, 1919, 3281, 3403, 3551
AcoI YGGCCR 3 cut(s) 1343, 1635, 2599
AcsI RAATTY 5 cut(s) 121, 519, 783, 1630, 1939
AcuI CTGAAG 8 cut(s) 137, 211, 386, 713, 2046, 2735, 3210, 3306
AcyI GRCGYC 1 cut(s) 2318
AdeI CACNNNGTG 1 cut(s) 2114
AfaI GTAC 8 cut(s) 416, 529, 1657, 1887, 2047, 2105, 2901, 3452
AfiI CCNNNNNNNGG 8 cut(s) 333, 568, 859, 1927, 2200, 2260, 2639, 2819
AflIII ACRYGT 3 cut(s) 2124, 3183, 3394
AgeI ACCGGT 1 cut(s) 3122
AhlI ACTAGT 1 cut(s) 775
AjnI CCWGG 9 cut(s) 99, 256, 1069, 2199, 2338, 2538, 2959, 3366, 3522
AjuI GAANNNNNNNTTGG 2 cut(s) 1214, 1246
Alw26I GTCTC 8 cut(s) 534, 1311, 1695, 1810, 2277, 2791, 2817, 3554
AlwI GGATC 5 cut(s) 1906, 1919, 3281, 3403, 3551
AlwNI CAGNNNCTG 4 cut(s) 724, 2696, 3175, 3529
Ama87I CYCGRG 2 cut(s) 1390, 2833
Aor13HI TCCGGA 1 cut(s) 3207
ApaI GGGCCC 1 cut(s) 3158
ApeKI GCWGC 5 cut(s) 6, 137, 1483, 1516, 2978
ApoI RAATTY 5 cut(s) 121, 519, 783, 1630, 1939
ArsI GACNNNNNNTTYG 4 cut(s) 605, 637, 2947, 2979
AsiGI ACCGGT 1 cut(s) 3122
Asp700I GAANNNNTTC 3 cut(s) 111, 122, 504
AspA2I CCTAGG 1 cut(s) 386
AspLEI GCGC 6 cut(s) 297, 877, 1273, 1296, 2221, 3342
AspS9I GGNCC 6 cut(s) 2191, 2203, 3154, 3155, 3218, 3446
AsuC2I CCSGG 2 cut(s) 2462, 3565
AvaI CYCGRG 2 cut(s) 1390, 2833
AvaII GGWCC 2 cut(s) 2191, 3446
AvrII CCTAGG 1 cut(s) 386
AxyI CCTNAGG 1 cut(s) 1848
BaeGI GKGCMC 1 cut(s) 3158
BalI TGGCCA 1 cut(s) 1637
BamHI GGATCC 1 cut(s) 1911
BanI GGYRCC 1 cut(s) 3119
BanII GRGCYC 1 cut(s) 3158
BbsI GAAGAC 5 cut(s) 217, 668, 1514, 2043, 3198
BbvCI CCTCAGC 1 cut(s) 2517
BbvI GCAGC 5 cut(s) 18, 149, 1470, 1528, 2965
BccI CCATC 4 cut(s) 562, 992, 1598, 3124
BceAI ACGGC 2 cut(s) 2586, 2641
BcgI CGANNNNNNTGC 4 cut(s) 1121, 1155, 1465, 1499
BciT130I CCWGG 9 cut(s) 101, 258, 1071, 2201, 2340, 2540, 2961, 3368, 3524
BciVI GTATCC 2 cut(s) 1986, 2257
BclI TGATCA 2 cut(s) 625, 2698
BcnI CCSGG 2 cut(s) 2462, 3565
BcoDI GTCTC 8 cut(s) 534, 1311, 1695, 1810, 2277, 2791, 2817, 3554
BcuI ACTAGT 1 cut(s) 775
BfaI CTAG 5 cut(s) 87, 387, 776, 1361, 2861
BfmI CTRYAG 4 cut(s) 1484, 2041, 2149, 3471
BfoI RGCGCY 2 cut(s) 298, 3343
BfuAI ACCTGC 2 cut(s) 1232, 1510
BfuI GTATCC 2 cut(s) 1986, 2257
BisI GCNGC 7 cut(s) 7, 138, 1292, 1343, 1484, 1517, 2979
BlnI CCTAGG 1 cut(s) 386
BlsI GCNGC 7 cut(s) 8, 139, 1293, 1344, 1485, 1518, 2980
BmcAI AGTACT 1 cut(s) 2047
Bme18I GGWCC 2 cut(s) 2191, 3446
BmeT110I CYCGRG 2 cut(s) 1390, 2833
BmgT120I GGNCC 6 cut(s) 2191, 2203, 3154, 3155, 3218, 3446
BmiI GGNNCC 6 cut(s) 1913, 2192, 3121, 3156, 3157, 3447
BmrI ACTGGG 4 cut(s) 1582, 1780, 2360, 3225
BmuI ACTGGG 4 cut(s) 1582, 1780, 2360, 3225
BpiI GAAGAC 5 cut(s) 217, 668, 1514, 2043, 3198
BpmI CTGGAG 2 cut(s) 1428, 2891
Bpu10I CCTNAGC 1 cut(s) 2517
BpuMI CCSGG 2 cut(s) 2462, 3565
Bsa29I ATCGAT 1 cut(s) 2010
BsaAI YACGTR 1 cut(s) 3397
BsaBI GATNNNNATC 1 cut(s) 624
BsaHI GRCGYC 1 cut(s) 2318
BsaI GGTCTC 2 cut(s) 1311, 3554
BsaJI CCNNGG 7 cut(s) 100, 328, 386, 1070, 2329, 2538, 3366
BsaWI WCCGGW 2 cut(s) 3122, 3207
BsaXI ACNNNNNCTCC 6 cut(s) 1430, 1460, 2519, 2549, 2668, 2698
Bsc4I CCNNNNNNNGG 8 cut(s) 333, 568, 859, 1927, 2200, 2260, 2639, 2819
Bse118I RCCGGY 1 cut(s) 3122
Bse21I CCTNAGG 1 cut(s) 1848
Bse8I GATNNNNATC 1 cut(s) 624
BseAI TCCGGA 1 cut(s) 3207
BseBI CCWGG 9 cut(s) 101, 258, 1071, 2201, 2340, 2540, 2961, 3368, 3524
BseCI ATCGAT 1 cut(s) 2010
BseDI CCNNGG 7 cut(s) 100, 328, 386, 1070, 2329, 2538, 3366
BseGI GGATG 9 cut(s) 597, 1003, 1045, 1216, 1420, 1449, 1561, 1723, 1827
BseJI GATNNNNATC 1 cut(s) 624
BseLI CCNNNNNNNGG 8 cut(s) 333, 568, 859, 1927, 2200, 2260, 2639, 2819
BseMII CTCAG 6 cut(s) 1132, 1828, 1862, 2484, 2508, 3367
BseRI GAGGAG 1 cut(s) 3352
BseSI GKGCMC 1 cut(s) 3158
BseXI GCAGC 5 cut(s) 18, 149, 1470, 1528, 2965
BsgI GTGCAG 3 cut(s) 675, 2267, 2701
Bsh1236I CGCG 1 cut(s) 969
BshNI GGYRCC 1 cut(s) 3119
BshTI ACCGGT 1 cut(s) 3122
BshVI ATCGAT 1 cut(s) 2010
BsiHKCI CYCGRG 2 cut(s) 1390, 2833
BsiSI CCGG 5 cut(s) 2461, 3123, 3208, 3457, 3565
BslFI GGGAC 6 cut(s) 331, 545, 1150, 1781, 2860, 3482
BslI CCNNNNNNNGG 8 cut(s) 333, 568, 859, 1927, 2200, 2260, 2639, 2819
BsmAI GTCTC 8 cut(s) 534, 1311, 1695, 1810, 2277, 2791, 2817, 3554
BsmFI GGGAC 6 cut(s) 331, 545, 1150, 1781, 2860, 3482
BsmI GAATGC 3 cut(s) 1027, 1397, 2404
Bso31I GGTCTC 2 cut(s) 1311, 3554
BsoBI CYCGRG 2 cut(s) 1390, 2833
Bsp120I GGGCCC 1 cut(s) 3154
Bsp1286I GDGCHC 1 cut(s) 3158
Bsp13I TCCGGA 1 cut(s) 3207
Bsp68I TCGCGA 1 cut(s) 969
BspACI CCGC 8 cut(s) 1182, 1291, 1342, 1481, 1504, 1559, 2551, 3093
BspCNI CTCAG 6 cut(s) 1131, 1827, 1861, 2485, 2509, 3366
BspDI ATCGAT 1 cut(s) 2010
BspEI TCCGGA 1 cut(s) 3207
BspFNI CGCG 1 cut(s) 969
BspHI TCATGA 1 cut(s) 2173
BspLI GGNNCC 6 cut(s) 1913, 2192, 3121, 3156, 3157, 3447
BspMAI CTGCAG 2 cut(s) 1488, 3475
BspMI ACCTGC 2 cut(s) 1232, 1510
BspPI GGATC 5 cut(s) 1906, 1919, 3281, 3403, 3551
BspT107I GGYRCC 1 cut(s) 3119
BspTNI GGTCTC 2 cut(s) 1311, 3554
BsrFI RCCGGY 1 cut(s) 3122
BssAI RCCGGY 1 cut(s) 3122
BssECI CCNNGG 7 cut(s) 100, 328, 386, 1070, 2329, 2538, 3366
BssNAI GTATAC 1 cut(s) 3109
BssNI GRCGYC 1 cut(s) 2318
BssT1I CCWWGG 3 cut(s) 328, 386, 2329
Bst1107I GTATAC 1 cut(s) 3109
Bst2UI CCWGG 9 cut(s) 101, 258, 1071, 2201, 2340, 2540, 2961, 3368, 3524
Bst6I CTCTTC 5 cut(s) 746, 1080, 2025, 3008, 3350
BstACI GRCGYC 1 cut(s) 2318
BstAPI GCANNNNNTGC 1 cut(s) 1197
BstBAI YACGTR 1 cut(s) 3397
BstC8I GCNNGC 7 cut(s) 335, 393, 937, 1141, 1473, 2223, 2840
BstDEI CTNAG 8 cut(s) 423, 1118, 1814, 1848, 2493, 2517, 2971, 3353
BstENI CCTNNNNNAGG 1 cut(s) 2198
BstF5I GGATG 9 cut(s) 597, 1003, 1045, 1216, 1420, 1449, 1561, 1723, 1827
BstFNI CGCG 1 cut(s) 969
BstH2I RGCGCY 2 cut(s) 298, 3343
BstHHI GCGC 6 cut(s) 297, 877, 1273, 1296, 2221, 3342
BstMAI GTCTC 8 cut(s) 534, 1311, 1695, 1810, 2277, 2791, 2817, 3554
BstNI CCWGG 9 cut(s) 101, 258, 1071, 2201, 2340, 2540, 2961, 3368, 3524
BstNSI RCATGY 5 cut(s) 395, 1286, 2128, 2842, 3187
BstSFI CTRYAG 4 cut(s) 1484, 2041, 2149, 3471
BstSLI GKGCMC 1 cut(s) 3158
BstUI CGCG 1 cut(s) 969
BstV1I GCAGC 5 cut(s) 18, 149, 1470, 1528, 2965
BstV2I GAAGAC 5 cut(s) 217, 668, 1514, 2043, 3198
BstX2I RGATCY 1 cut(s) 1911
BstXI CCANNNNNNTGG 1 cut(s) 345
BstYI RGATCY 1 cut(s) 1911
BstZ17I GTATAC 1 cut(s) 3109
Bsu15I ATCGAT 1 cut(s) 2010
Bsu36I CCTNAGG 1 cut(s) 1848
BsuI GTATCC 2 cut(s) 1986, 2257
BsuTUI ATCGAT 1 cut(s) 2010
BtgZI GCGATG 2 cut(s) 984, 3040
BtsCI GGATG 9 cut(s) 597, 1003, 1045, 1216, 1420, 1449, 1561, 1723, 1827
BtsI GCAGTG 2 cut(s) 1733, 2689
BtsIMutI CAGTG 9 cut(s) 1570, 1641, 1733, 2110, 2689, 3118, 3213, 3252, 3361
BtuMI TCGCGA 1 cut(s) 969
BveI ACCTGC 2 cut(s) 1232, 1510
Cac8I GCNNGC 7 cut(s) 335, 393, 937, 1141, 1473, 2223, 2840
CaiI CAGNNNCTG 4 cut(s) 724, 2696, 3175, 3529
CciI TCATGA 1 cut(s) 2173
CfoI GCGC 6 cut(s) 297, 877, 1273, 1296, 2221, 3342
Cfr10I RCCGGY 1 cut(s) 3122
Cfr13I GGNCC 6 cut(s) 2191, 2203, 3154, 3155, 3218, 3446
ClaI ATCGAT 1 cut(s) 2010
CseI GACGC 1 cut(s) 3161
Csp6I GTAC 8 cut(s) 415, 528, 1656, 1886, 2046, 2104, 2900, 3451
CspAI ACCGGT 1 cut(s) 3122
CviQI GTAC 8 cut(s) 415, 528, 1656, 1886, 2046, 2104, 2900, 3451
DdeI CTNAG 8 cut(s) 423, 1118, 1814, 1848, 2493, 2517, 2971, 3353
DraI TTTAAA 1 cut(s) 1963
DraIII CACNNNGTG 1 cut(s) 2114
DrdI GACNNNNNNGTC 1 cut(s) 2317
DseDI GACNNNNNNGTC 1 cut(s) 2317
EaeI YGGCCR 3 cut(s) 1343, 1635, 2599
Eam1104I CTCTTC 5 cut(s) 746, 1080, 2025, 3008, 3350
EarI CTCTTC 5 cut(s) 746, 1080, 2025, 3008, 3350
EciI GGCGGA 1 cut(s) 2566
Eco130I CCWWGG 3 cut(s) 328, 386, 2329
Eco147I AGGCCT 2 cut(s) 1125, 1307
Eco24I GRGCYC 1 cut(s) 3158
Eco31I GGTCTC 2 cut(s) 1311, 3554
Eco32I GATATC 1 cut(s) 2665
Eco47I GGWCC 2 cut(s) 2191, 3446
Eco57I CTGAAG 8 cut(s) 137, 211, 386, 713, 2046, 2735, 3210, 3306
Eco81I CCTNAGG 1 cut(s) 1848
Eco88I CYCGRG 2 cut(s) 1390, 2833
EcoNI CCTNNNNNAGG 1 cut(s) 2198
EcoO109I RGGNCCY 2 cut(s) 2191, 3155
EcoRII CCWGG 9 cut(s) 99, 256, 1069, 2199, 2338, 2538, 2959, 3366, 3522
EcoRV GATATC 1 cut(s) 2665
EcoT14I CCWWGG 3 cut(s) 328, 386, 2329
EcoT22I ATGCAT 2 cut(s) 2844, 2941
EcoT38I GRGCYC 1 cut(s) 3158
ErhI CCWWGG 3 cut(s) 328, 386, 2329
FalI AAGNNNNNCTT 4 cut(s) 566, 598, 1127, 1159
FaqI GGGAC 6 cut(s) 331, 545, 1150, 1781, 2860, 3482
FauI CCCGC 1 cut(s) 1566
FauNDI CATATG 1 cut(s) 2635
FbaI TGATCA 2 cut(s) 625, 2698
FblI GTMKAC 2 cut(s) 842, 3108
Fnu4HI GCNGC 7 cut(s) 7, 138, 1292, 1343, 1484, 1517, 2979
FokI GGATG 9 cut(s) 604, 1010, 1052, 1223, 1407, 1456, 1548, 1730, 1834
FriOI GRGCYC 1 cut(s) 3158
Fsp4HI GCNGC 7 cut(s) 7, 138, 1292, 1343, 1484, 1517, 2979
FspBI CTAG 5 cut(s) 87, 387, 776, 1361, 2861
GlaI GCGC 6 cut(s) 296, 876, 1272, 1295, 2220, 3341
GluI GCNGC 7 cut(s) 7, 138, 1292, 1343, 1484, 1517, 2979
GsuI CTGGAG 2 cut(s) 1428, 2891
HaeII RGCGCY 2 cut(s) 298, 3343
HapII CCGG 5 cut(s) 2461, 3123, 3208, 3457, 3565
HgaI GACGC 1 cut(s) 3161
HhaI GCGC 6 cut(s) 297, 877, 1273, 1296, 2221, 3342
Hin1I GRCGYC 1 cut(s) 2318
Hin6I GCGC 6 cut(s) 295, 875, 1271, 1294, 2219, 3340
HinP1I GCGC 6 cut(s) 295, 875, 1271, 1294, 2219, 3340
HincII GTYRAC 5 cut(s) 37, 843, 2524, 2608, 2966
HindII GTYRAC 5 cut(s) 37, 843, 2524, 2608, 2966
HpaII CCGG 5 cut(s) 2461, 3123, 3208, 3457, 3565
Hpy99I CGWCG 5 cut(s) 53, 274, 449, 1830, 2323
HpyCH4IV ACGT 9 cut(s) 413, 600, 1828, 2318, 2476, 3295, 3396, 3433, 3495
HpyF3I CTNAG 8 cut(s) 423, 1118, 1814, 1848, 2493, 2517, 2971, 3353
HpySE526I ACGT 9 cut(s) 413, 600, 1828, 2318, 2476, 3295, 3396, 3433, 3495
Hsp92I GRCGYC 1 cut(s) 2318
HspAI GCGC 6 cut(s) 295, 875, 1271, 1294, 2219, 3340
Kpn2I TCCGGA 1 cut(s) 3207
Ksp22I TGATCA 2 cut(s) 625, 2698
LmnI GCTCC 6 cut(s) 1409, 2021, 2693, 3317, 3506, 3526
Lsp1109I GCAGC 5 cut(s) 18, 149, 1470, 1528, 2965
MaeI CTAG 5 cut(s) 87, 387, 776, 1361, 2861
MaeII ACGT 9 cut(s) 413, 600, 1828, 2318, 2476, 3295, 3396, 3433, 3495
MaeIII GTNAC 9 cut(s) 56, 322, 611, 1170, 1375, 2507, 3296, 3356, 3513
MfeI CAATTG 1 cut(s) 1347
MflI RGATCY 1 cut(s) 1911
MhlI GDGCHC 1 cut(s) 3158
MlsI TGGCCA 1 cut(s) 1637
MluNI TGGCCA 1 cut(s) 1637
MlyI GAGTC 3 cut(s) 2528, 2882, 3353
MmeI TCCRAC 7 cut(s) 603, 760, 1212, 1522, 1756, 2301, 3436
Mox20I TGGCCA 1 cut(s) 1637
Mph1103I ATGCAT 2 cut(s) 2844, 2941
MroI TCCGGA 1 cut(s) 3207
MroXI GAANNNNTTC 3 cut(s) 111, 122, 504
MscI TGGCCA 1 cut(s) 1637
MseI TTAA 8 cut(s) 93, 486, 524, 764, 1962, 2061, 2144, 3574
MslI CAYNNNNRTG 3 cut(s) 2178, 2453, 3149
Msp20I TGGCCA 1 cut(s) 1637
MspA1I CMGCKG 3 cut(s) 1184, 1483, 2096
MspI CCGG 5 cut(s) 2461, 3123, 3208, 3457, 3565
MunI CAATTG 1 cut(s) 1347
Mva1269I GAATGC 3 cut(s) 1027, 1397, 2404
MvaI CCWGG 9 cut(s) 101, 258, 1071, 2201, 2340, 2540, 2961, 3368, 3524
MvnI CGCG 1 cut(s) 969
NciI CCSGG 2 cut(s) 2462, 3565
NdeI CATATG 1 cut(s) 2635
NlaIV GGNNCC 6 cut(s) 1913, 2192, 3121, 3156, 3157, 3447
NmeAIII GCCGAG 1 cut(s) 2195
NmuCI GTSAC 4 cut(s) 56, 611, 3296, 3356
NruI TCGCGA 1 cut(s) 969
NsiI ATGCAT 2 cut(s) 2844, 2941
NspI RCATGY 5 cut(s) 395, 1286, 2128, 2842, 3187
PaeI GCATGC 2 cut(s) 395, 2842
PaeR7I CTCGAG 2 cut(s) 1390, 2833
PagI TCATGA 1 cut(s) 2173
PaqCI CACCTGC 1 cut(s) 1232
PceI AGGCCT 2 cut(s) 1125, 1307
PciI ACATGT 2 cut(s) 2124, 3183
PctI GAATGC 3 cut(s) 1027, 1397, 2404
PdmI GAANNNNTTC 3 cut(s) 111, 122, 504
PfeI GAWTC 8 cut(s) 1477, 1670, 1790, 2170, 2430, 2687, 2994, 3178
PinAI ACCGGT 1 cut(s) 3122
PkrI GCNGC 7 cut(s) 8, 139, 1293, 1344, 1485, 1518, 2980
PleI GAGTC 3 cut(s) 2528, 2881, 3353
PpsI GAGTC 3 cut(s) 2528, 2881, 3353
Ppu21I YACGTR 1 cut(s) 3397
PpuMI RGGWCCY 1 cut(s) 2191
PscI ACATGT 2 cut(s) 2124, 3183
PsiI TTATAA 2 cut(s) 644, 2927
Psp5II RGGWCCY 1 cut(s) 2191
Psp6I CCWGG 9 cut(s) 99, 256, 1069, 2199, 2338, 2538, 2959, 3366, 3522
PspGI CCWGG 9 cut(s) 99, 256, 1069, 2199, 2338, 2538, 2959, 3366, 3522
PspN4I GGNNCC 6 cut(s) 1913, 2192, 3121, 3156, 3157, 3447
PspOMI GGGCCC 1 cut(s) 3154
PspPI GGNCC 6 cut(s) 2191, 2203, 3154, 3155, 3218, 3446
PspPPI RGGWCCY 1 cut(s) 2191
PsrI GAACNNNNNNTAC 2 cut(s) 398, 430
PstI CTGCAG 2 cut(s) 1488, 3475
PstNI CAGNNNCTG 4 cut(s) 724, 2696, 3175, 3529
PsuI RGATCY 1 cut(s) 1911
PvuII CAGCTG 1 cut(s) 2096
RruI TCGCGA 1 cut(s) 969
RsaI GTAC 8 cut(s) 416, 529, 1657, 1887, 2047, 2105, 2901, 3452
RsaNI GTAC 8 cut(s) 415, 528, 1656, 1886, 2046, 2104, 2900, 3451
RseI CAYNNNNRTG 3 cut(s) 2178, 2453, 3149
SalI GTCGAC 1 cut(s) 841
SaqAI TTAA 8 cut(s) 93, 486, 524, 764, 1962, 2061, 2144, 3574
SatI GCNGC 7 cut(s) 7, 138, 1292, 1343, 1484, 1517, 2979
Sau96I GGNCC 6 cut(s) 2191, 2203, 3154, 3155, 3218, 3446
ScaI AGTACT 1 cut(s) 2047
SchI GAGTC 3 cut(s) 2528, 2882, 3353
SduI GDGCHC 1 cut(s) 3158
SfcI CTRYAG 4 cut(s) 1484, 2041, 2149, 3471
Sfr274I CTCGAG 2 cut(s) 1390, 2833
SinI GGWCC 2 cut(s) 2191, 3446
SlaI CTCGAG 2 cut(s) 1390, 2833
SmiMI CAYNNNNRTG 3 cut(s) 2178, 2453, 3149
SmlI CTYRAG 2 cut(s) 1390, 2833
SmoI CTYRAG 2 cut(s) 1390, 2833
SpeI ACTAGT 1 cut(s) 775
SphI GCATGC 2 cut(s) 395, 2842
SseBI AGGCCT 2 cut(s) 1125, 1307
SsiI CCGC 8 cut(s) 1182, 1291, 1342, 1481, 1504, 1559, 2551, 3093
SspMI CTAG 5 cut(s) 87, 387, 776, 1361, 2861
StuI AGGCCT 2 cut(s) 1125, 1307
StyI CCWWGG 3 cut(s) 328, 386, 2329
TaiI ACGT 9 cut(s) 416, 603, 1831, 2321, 2479, 3298, 3399, 3436, 3498
TatI WGTACW 4 cut(s) 527, 2045, 2103, 2899
TauI GCSGC 2 cut(s) 1294, 1345
TfiI GAWTC 8 cut(s) 1477, 1670, 1790, 2170, 2430, 2687, 2994, 3178
Tru1I TTAA 8 cut(s) 93, 486, 524, 764, 1962, 2061, 2144, 3574
Tru9I TTAA 8 cut(s) 93, 486, 524, 764, 1962, 2061, 2144, 3574
TscAI CASTG 9 cut(s) 1577, 1648, 1740, 2117, 2689, 3118, 3220, 3252, 3361
TseFI GTSAC 4 cut(s) 56, 611, 3296, 3356
TseI GCWGC 5 cut(s) 6, 137, 1483, 1516, 2978
Tsp45I GTSAC 4 cut(s) 56, 611, 3296, 3356
TspDTI ATGAA 9 cut(s) 84, 190, 443, 608, 826, 876, 1884, 2471, 3139
TspGWI ACGGA 6 cut(s) 998, 1968, 2784, 2934, 3330, 3438
TspRI CASTG 9 cut(s) 1577, 1648, 1740, 2117, 2689, 3118, 3220, 3252, 3361
VpaK11BI GGWCC 2 cut(s) 2191, 3446
XagI CCTNNNNNAGG 1 cut(s) 2198
XapI RAATTY 5 cut(s) 121, 519, 783, 1630, 1939
XceI RCATGY 5 cut(s) 395, 1286, 2128, 2842, 3187
XhoI CTCGAG 2 cut(s) 1390, 2833
XmaJI CCTAGG 1 cut(s) 386
XmiI GTMKAC 2 cut(s) 842, 3108
XmnI GAANNNNTTC 3 cut(s) 111, 122, 504
XspI CTAG 5 cut(s) 87, 387, 776, 1361, 2861
ZraI GACGTC 1 cut(s) 2319
ZrmI AGTACT 1 cut(s) 2047
Zsp2I ATGCAT 2 cut(s) 2844, 2941
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.