Rw0G001490

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00043
OG0002206
Physical Location & Seq
Reverse (-)
170793 .. 177038
6246 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G001490.1

Sequence Viewer

Length: 3453 bp
ATGAGTAGTAAGGGGGTAAGCTTTTCCAATCGGGTTGACATGGATATTGATGATGATGATGAATCAATTGATTTGACTAAGGCATCAGTTTCATTCTTCAACCAGTATGGCCTCATTAGTCACCAAATAAACTCGTACAATGACTTCATTAAAAATGGTATACAAAGAGTCTTTGATAGTTTTGATGAGATTATAGTTGAGCCTGGGTATGACCCGTCAAACAAGGGGAATGCAGAATGGCGGTATGCTTCCTATTATATGCTACCTAGGCATGCTCGGCTTCAAAATATGACTTACTCAGCTAAGATCAAAGTGAACGTTACTATTGAGGATTCGTTTATATTTCTAAACTTCTTAAACCTTCTTATTTATCTCTCTTTTTTTTATGTACATGTATATACTCAAAATCTTGTCAGAAGCGAAAAGTTTAAAACTGGAGAAGAACAATGTCTTGACAAGCAGATTGTGACTACGGAGAGTAGAGATGTCTTTATTGGTAAGCTCCCAGTGATGGTGAAGTCTGACTTGTGTTGGCTGAAAGACGAGAGAGCTGACTGTGAATTTGACCATGGAGGTTATTTTATAATCAAGGGTGCAGAGAAGACCTTCATTGCGCAAGAGCAGACCTGTCTGAAGAGACTTCTAATTACAAGTAATCAGGGGCTCACAGTAGCATATAGGTCTGATGTCAAGCAGCATGGACTAATAATAAGATTATCTGGGATTTCTCAACTTGAAAACATTGAAGGATTAGAGAAAGTTTTGTCAGTGTACTTTATGTCAGCAGAAATCCCTGTCTGGATTTGGTTTTTTGCCCTTGGGGTTTCTTCGGACAATGAAGTTATAGATCTGATTGATTATGGCAAAGAAGATGCAAGCATTTCAAACATTCTAGTTGCCTCAATTCTTGATGCTGACCAACGAAAAGATATCTTCCGAAGAGGGAAGAATGCTCTCAGATATGTTGAAGAGATGATAAAGAATACCACGTTTCCTCCTTGGGAAAGCATTGAAGAATGCATCAGCATGTATCTGTTCCCCAATCTGAAAGGCATGAAGCGAAAGGCTCGGTTTCTTGGCTATATGAACAAGAGGCTTCACTTGGCTGGTGAGCTTCTAGAGCGAGAGCTGAAGGCACACATAGGACATGCAGGGAGGTGCATGGCAAAGGCTTTGCAGAGAGACCTTTGTGGTTACCGTGTTGTCCGCCCGGTTGAGCAATATATAGATGCTACAATAGTAACTAATGGCATCTCCAGAGCATTCTCAACAGGAGCGTGGTGTCATCCTTTCAAGACTATGGAGAGGATATCTGGTGTGGTGGTAAACGTTGGGAAAACAAATCCACTGCAAACAATGGTAGATATGAGGAAGACACGCCTCCAAGTTCAATACATGGGGAAGGTTCGAGATGCCAGATATCCGCATACTTCTCACTGGGGTAGGGTTTTCTTTATCTCAACCCCAGATGGTGAGAATTGTGGGCTTGTGAAAAATTTGGCTACCACTGCACTGATAAGTACAAACATATTGGAGCCCATATTGCCCGAGTTTTTCTCTTGTGGCATGGAGGAGCTGGTAGATGATACTTCAACATCACTTGATGGGAAGAATAAAGTTTTTCTCAATGGGGATTGGGTTGGCGTTTGTGAAGATTCCGGTGATTTTGTCACGAGACTAAGGACATTGAGACGCAGGAAAAAGTTGCTACAGCAGATGGAAATCAAAAGAGATGAGCAGCTCTGGGAAGTACGAATATATTCTGATGCTGGAAGGATTCTACGTCCTTTCCTGGTAGTTAAGAATTTGAATAAGATTAAAGCATCCAAGGGAAAAAAACACACGTTCCAAGATCTTCTTGGCGATGGAATAATCGAGCTCATTGGAGCTGAAGAAGAAGAGGATTGCTGTACAGCATGGGACGTCAGGCATCTATTTATGGAAGACAATGGGAAGTCAGCAGTCAAGTACACACACTGTGAACTGGACCTGTCATTTTTGTTAGGCTTAAGTTGTGGTATCATTCCCTTTTCAAACCATGATCATGCAAGGAGGGTTCTATACCAGTCTCAGAAGCACTCTCAGCAGGTCATTGGTTTTTCTACCACAAATCCTAACCACAGAGTGGTTACTCTGTCTCACCAACTATGCTACCCTCAGAGGCCGCTTTTTTGTAATATGATATCTGATTGCCTTGGCCAACCAGGAAACCCAGTGAGCCATAACAGAGTTATGTCCAAGCCTCAATACTTTAATGGCCAAAATGCAATTGTTGCATTCAATGTGCACCTAGGATATAACCAAGAAGATTCTATTGTGATGAACCGCGCATCTGTGCAACGTGGCATGTTCAAATCTGAGCACATACGGAGCTACAAGGCTGAAGCTGAAAACAAGTTCTCATTGGGAAAAAGGAGGAATCCAGATGACTGTATAAACTTTGGCAAAATACAGAGTAGATTTGGAAGTGTTGACAGCCTTGATGATGATGGATTCCCTCGTATAGGTGCATATCTGCTGAGTGGTGATATTATTATTGGGAGGTGCAGTGAATCTGGTACTTATCATAGCATAAAACTGAGGCATACTGAAAGAGGAAATGTTCAGAAGGTTGTACTTTCCTCTAATGACGAGGGAAAGAATTTTGGTGTTGTATCCTTAAGACAGACAAGTTCTGAAGCATGCACGGGTGTCCTAGGCTTTTTGGAGTCTGAAGAGAACTTCCCCTTTACAATACAGGGGATTGTTCCAGATATTGTAATTAATCCACATGCTTTTCCTTCACGACAAGCTCCTGGTCAACTCCTAGAGGCTGCTTTGGGGAAGGGGATTGCCTGTGGTGGCTCAATGAGATATGCAACTCCTTTCTCTACTCCCTCTGTTGATGACATCACAGATCAGCTTCATGGAGCTGGATTTCAAAGATGGGGAAATGAGAGTATACAGTGGTCAAACTGGTCAAATGGTTCGCCAACTGATATTTATGGGCCCAACATTTTATCAACGTCTCATTCACATTCTCATTCACATTTTAGGAACATTGGGCCAGTTCACCCTCTCACCCGGCAGCCAGTGGCAGACAGGAAGAGATTTTGTGGGGTAAAGTTTGGTGTGATGGAGCTCGACTGCCTTATAGCCCATGGAGCATCAGCAAACTTACATGAACGCCTTTTCACTCTTTGTGACTCCTCCCAAATGCATGCTTGCCAGAAATGCAAAAATGCGGCTAATGTGATAGATGGAACAGTTGATGGCCGTAGAATCCGGGGGCCCTATTGCCTGGTCTGTAAGTCTGTTGATGACATTGTCAGGTTGAATGTTCCATACGGGGCCAAGTTATTGTGCCAGGAACTTTTCAGTGTGGGCATAAGTCTCAAGTTTGAGACCAGGCTCTGCAGAGTGAGCACTAGAACTCTGTTTTCGGAGCGCTATAGAACTTTGTTTTGTAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000228 GO:0000418 GO:0000419 GO:0000428 GO:0000785 GO:0000790 GO:0000792 GO:0002682 GO:0003674 GO:0003824 GO:0003899 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005720 GO:0005730 GO:0005736 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009605 GO:0009607 GO:0009620 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016020 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019222 GO:0019438 GO:0030422 GO:0030880 GO:0031047 GO:0031050 GO:0031974 GO:0031981 GO:0032259 GO:0032774 GO:0032991 GO:0034062 GO:0034641 GO:0034654 GO:0035194 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043331 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044451 GO:0044452 GO:0044454 GO:0044464 GO:0044728 GO:0046483 GO:0048519 GO:0048583 GO:0050776 GO:0050789 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0055029 GO:0060255 GO:0061695 GO:0065007 GO:0070013 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0097747 GO:0098542 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1901698 GO:1901699 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko01100 ko03020 ko05016 ko05169 map00230 map00240 map01100 map03020 map05016 map05169
Pfam Domains
Protein Families
RNA_pol_Rpb2_1 RNA_pol_Rpb2_2 RNA_pol_Rpb2_3 RNA_pol_Rpb2_4 RNA_pol_Rpb2_5 RNA_pol_Rpb2_6 RNA_pol_Rpb2_7

Protein Analysis

1150

Amino Acids

130.08

Weight (kDa)

8.15

Isoelectric Point (pI)

39.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNA_pol_Rpb2_1 PF04563 37 - 396 2.3e-21 RNA polymerase beta subunit
RNA_pol_Rpb2_3 PF04565 446 - 505 4.2e-14 RNA polymerase Rpb2, domain 3
RNA_pol_Rpb2_4 PF04566 540 - 601 3.1e-20 RNA polymerase Rpb2, domain 4
RNA_pol_Rpb2_5 PF04567 616 - 663 4.4e-08 RNA polymerase Rpb2, domain 5
RNA_pol_Rpb2_6 PF00562 672 - 1030 9.7e-64 RNA polymerase Rpb2, domain 6
RNA_pol_Rpb2_7 PF04560 1033 - 1128 3.5e-20 RNA polymerase Rpb2, domain 7
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 584
AatII GACGTC 1 cut(s) 1926
Acc16I TGCGCA 1 cut(s) 615
Acc36I ACCTGC 1 cut(s) 2077
AccB7I CCANNNNNTGG 1 cut(s) 2125
AccI GTMKAC 2 cut(s) 160, 2941
AccII CGCG 1 cut(s) 2328
AciI CCGC 6 cut(s) 241, 1207, 1424, 2165, 2326, 3224
AclI AACGTT 2 cut(s) 318, 1329
AcoI YGGCCR 3 cut(s) 2197, 2257, 3253
AcsI RAATTY 4 cut(s) 560, 1495, 1804, 2641
AcuI CTGAAG 6 cut(s) 653, 1151, 1911, 2403, 2697, 2733
AcyI GRCGYC 1 cut(s) 1923
AdeI CACNNNGTG 2 cut(s) 1979, 2125
AfaI GTAC 9 cut(s) 137, 390, 773, 1522, 1752, 1912, 1970, 2560, 2616
AfeI AGCGCT 1 cut(s) 3428
AfiI CCNNNNNNNGG 3 cut(s) 511, 2125, 2504
AflII CTTAAG 2 cut(s) 2008, 2659
AflIII ACRYGT 2 cut(s) 391, 1842
AjnI CCWGG 7 cut(s) 202, 1791, 2203, 2794, 3279, 3345, 3386
Alw21I GWGCWC 5 cut(s) 1881, 2289, 2364, 3123, 3407
Alw26I GTCTC 9 cut(s) 631, 1176, 1669, 1684, 2073, 2142, 3012, 3377, 3377
Alw44I GTGCAC 1 cut(s) 2285
AlwNI CAGNNNCTG 1 cut(s) 3393
Ama87I CYCGRG 1 cut(s) 1547
Aor51HI AGCGCT 1 cut(s) 3428
AoxI GGCC 9 cut(s) 109, 2162, 2197, 2257, 2987, 3044, 3253, 3269, 3330
ApaI GGGCCC 2 cut(s) 2991, 3273
ApaLI GTGCAC 1 cut(s) 2285
ApeKI GCWGC 4 cut(s) 694, 1738, 2813, 3067
ApoI RAATTY 4 cut(s) 560, 1495, 1804, 2641
ArsI GACNNNNNNTTYG 2 cut(s) 545, 577
AseI ATTAAT 1 cut(s) 2763
Asp700I GAANNNNTTC 2 cut(s) 605, 1759
AspA2I CCTAGG 3 cut(s) 266, 2290, 2695
AspLEI GCGC 3 cut(s) 616, 2330, 3429
AspS9I GGNCC 7 cut(s) 1987, 2987, 2988, 3044, 3269, 3270, 3330
AsuC2I CCSGG 3 cut(s) 1211, 3064, 3266
AsuHPI GGTGA 9 cut(s) 113, 526, 1121, 1484, 1673, 2132, 2537, 3044, 3052
AvaI CYCGRG 1 cut(s) 1547
AvaII GGWCC 1 cut(s) 1987
AvrII CCTAGG 3 cut(s) 266, 2290, 2695
BaeGI GKGCMC 3 cut(s) 2289, 2991, 3273
BalI TGGCCA 2 cut(s) 2199, 2259
BanII GRGCYC 6 cut(s) 666, 1539, 1881, 2991, 3123, 3273
BarI GAAGNNNNNNTAC 2 cut(s) 128, 160
BauI CACGAG 1 cut(s) 1672
BbsI GAAGAC 3 cut(s) 608, 1379, 1950
Bbv12I GWGCWC 5 cut(s) 1881, 2289, 2364, 3123, 3407
BbvI GCAGC 4 cut(s) 706, 1750, 2800, 3079
BceAI ACGGC 1 cut(s) 3240
BciT130I CCWGG 7 cut(s) 204, 1793, 2205, 2796, 3281, 3347, 3388
BciVI GTATCC 1 cut(s) 2665
BclI TGATCA 1 cut(s) 2041
BcnI CCSGG 3 cut(s) 1211, 3064, 3266
BcoDI GTCTC 9 cut(s) 631, 1176, 1669, 1684, 2073, 2142, 3012, 3377, 3377
BfaI CTAG 7 cut(s) 267, 893, 1118, 2291, 2696, 2807, 3408
BfmI CTRYAG 3 cut(s) 1709, 3394, 3430
BfoI RGCGCY 1 cut(s) 3430
BfrI CTTAAG 2 cut(s) 2008, 2659
BfuAI ACCTGC 1 cut(s) 2077
BfuI GTATCC 1 cut(s) 2665
BglII AGATCT 2 cut(s) 847, 1852
BisI GCNGC 6 cut(s) 695, 1739, 2165, 2814, 3068, 3225
BlnI CCTAGG 3 cut(s) 266, 2290, 2695
BlsI GCNGC 6 cut(s) 696, 1740, 2166, 2815, 3069, 3226
Bme18I GGWCC 1 cut(s) 1987
BmeT110I CYCGRG 1 cut(s) 1547
BmgT120I GGNCC 7 cut(s) 1987, 2987, 2988, 3044, 3269, 3270, 3330
BmiI GGNNCC 5 cut(s) 1536, 2989, 3270, 3271, 3331
BmrI ACTGGG 3 cut(s) 500, 1447, 2207
BmuI ACTGGG 3 cut(s) 500, 1447, 2207
BpiI GAAGAC 3 cut(s) 608, 1379, 1950
BplI GAGNNNNNCTC 2 cut(s) 1541, 1573
BpmI CTGGAG 2 cut(s) 456, 1240
BpuEI CTTGAG 1 cut(s) 3359
BpuMI CCSGG 3 cut(s) 1211, 3064, 3266
BsaBI GATNNNNATC 1 cut(s) 1721
BsaHI GRCGYC 1 cut(s) 1923
BsaI GGTCTC 2 cut(s) 1176, 3377
BsaWI WCCGGW 1 cut(s) 1658
BsaXI ACNNNNNCTCC 4 cut(s) 979, 1009, 2533, 2563
Bsc4I CCNNNNNNNGG 3 cut(s) 511, 2125, 2504
Bse3DI GCAATG 1 cut(s) 609
Bse8I GATNNNNATC 1 cut(s) 1721
BseBI CCWGG 7 cut(s) 204, 1793, 2205, 2796, 3281, 3347, 3388
BseGI GGATG 2 cut(s) 1285, 1823
BseJI GATNNNNATC 1 cut(s) 1721
BseLI CCNNNNNNNGG 3 cut(s) 511, 2125, 2504
BseMI GCAATG 1 cut(s) 609
BseMII CTCAG 8 cut(s) 312, 970, 2084, 2096, 2171, 2349, 2510, 2570
BseRI GAGGAG 2 cut(s) 1586, 3178
BseSI GKGCMC 3 cut(s) 2289, 2991, 3273
BseXI GCAGC 4 cut(s) 706, 1750, 2800, 3079
BsgI GTGCAG 3 cut(s) 615, 1494, 2566
Bsh1236I CGCG 1 cut(s) 2328
BshFI GGCC 9 cut(s) 111, 2164, 2199, 2259, 2989, 3046, 3255, 3271, 3332
BsiHKAI GWGCWC 5 cut(s) 1881, 2289, 2364, 3123, 3407
BsiHKCI CYCGRG 1 cut(s) 1547
BsiSI CCGG 4 cut(s) 1211, 1659, 3064, 3265
BslFI GGGAC 1 cut(s) 1934
BslI CCNNNNNNNGG 3 cut(s) 511, 2125, 2504
BsmAI GTCTC 9 cut(s) 631, 1176, 1669, 1684, 2073, 2142, 3012, 3377, 3377
BsmBI CGTCTC 2 cut(s) 1684, 3012
BsmFI GGGAC 1 cut(s) 1934
BsmI GAATGC 5 cut(s) 235, 955, 1022, 1262, 2276
BsnI GGCC 9 cut(s) 111, 2164, 2199, 2259, 2989, 3046, 3255, 3271, 3332
Bso31I GGTCTC 2 cut(s) 1176, 3377
BsoBI CYCGRG 1 cut(s) 1547
Bsp120I GGGCCC 2 cut(s) 2987, 3269
Bsp1286I GDGCHC 9 cut(s) 666, 1539, 1881, 2289, 2364, 2991, 3123, 3273, 3407
Bsp1407I TGTACA 2 cut(s) 388, 1910
Bsp143I GATC 5 cut(s) 306, 847, 1852, 2041, 2896
Bsp19I CCATGG 2 cut(s) 568, 3139
BspACI CCGC 6 cut(s) 241, 1207, 1424, 2165, 2326, 3224
BspANI GGCC 9 cut(s) 111, 2164, 2199, 2259, 2989, 3046, 3255, 3271, 3332
BspCNI CTCAG 8 cut(s) 311, 969, 2083, 2095, 2170, 2350, 2511, 2571
BspFNI CGCG 1 cut(s) 2328
BspLI GGNNCC 5 cut(s) 1536, 2989, 3270, 3271, 3331
BspMAI CTGCAG 1 cut(s) 3398
BspMI ACCTGC 1 cut(s) 2077
BspTI CTTAAG 2 cut(s) 2008, 2659
BspTNI GGTCTC 2 cut(s) 1176, 3377
BsrDI GCAATG 1 cut(s) 609
BsrGI TGTACA 2 cut(s) 388, 1910
BssMI GATC 5 cut(s) 306, 847, 1852, 2041, 2896
BssNAI GTATAC 2 cut(s) 161, 2942
BssNI GRCGYC 1 cut(s) 1923
BssSI CACGAG 1 cut(s) 1672
BssT1I CCWWGG 9 cut(s) 266, 568, 817, 998, 1827, 2194, 2290, 2695, 3139
Bst1107I GTATAC 2 cut(s) 161, 2942
Bst2BI CACGAG 1 cut(s) 1672
Bst2UI CCWGG 7 cut(s) 204, 1793, 2205, 2796, 3281, 3347, 3388
Bst4CI ACNGT 7 cut(s) 557, 670, 1199, 1979, 2432, 2946, 3247
Bst6I CTCTTC 6 cut(s) 629, 934, 963, 1893, 2709, 3080
BstACI GRCGYC 1 cut(s) 1923
BstAFI CTTAAG 2 cut(s) 2008, 2659
BstAPI GCANNNNNTGC 1 cut(s) 2273
BstAUI TGTACA 2 cut(s) 388, 1910
BstC8I GCNNGC 5 cut(s) 273, 877, 2683, 3201, 3205
BstDSI CCRYGG 2 cut(s) 568, 3139
BstEII GGTNACC 1 cut(s) 1193
BstENI CCTNNNNNAGG 1 cut(s) 2502
BstF5I GGATG 2 cut(s) 1285, 1823
BstFNI CGCG 1 cut(s) 2328
BstH2I RGCGCY 1 cut(s) 3430
BstHHI GCGC 3 cut(s) 616, 2330, 3429
BstKTI GATC 5 cut(s) 309, 850, 1855, 2044, 2899
BstMAI GTCTC 9 cut(s) 631, 1176, 1669, 1684, 2073, 2142, 3012, 3377, 3377
BstMBI GATC 5 cut(s) 306, 847, 1852, 2041, 2896
BstNI CCWGG 7 cut(s) 204, 1793, 2205, 2796, 3281, 3347, 3388
BstNSI RCATGY 8 cut(s) 275, 395, 1030, 1151, 2350, 2685, 2774, 3203
BstPI GGTNACC 1 cut(s) 1193
BstSFI CTRYAG 3 cut(s) 1709, 3394, 3430
BstSLI GKGCMC 3 cut(s) 2289, 2991, 3273
BstUI CGCG 1 cut(s) 2328
BstV1I GCAGC 4 cut(s) 706, 1750, 2800, 3079
BstV2I GAAGAC 3 cut(s) 608, 1379, 1950
BstX2I RGATCY 2 cut(s) 847, 1852
BstYI RGATCY 2 cut(s) 847, 1852
BstZ17I GTATAC 2 cut(s) 161, 2942
BsuI GTATCC 1 cut(s) 2665
BsuRI GGCC 9 cut(s) 111, 2164, 2199, 2259, 2989, 3046, 3255, 3271, 3332
BtgI CCRYGG 2 cut(s) 568, 3139
BtgZI GCGATG 1 cut(s) 1878
BtsCI GGATG 2 cut(s) 1285, 1823
BtsI GCAGTG 3 cut(s) 1346, 1506, 2554
BveI ACCTGC 1 cut(s) 2077
Cac8I GCNNGC 5 cut(s) 273, 877, 2683, 3201, 3205
CaiI CAGNNNCTG 1 cut(s) 3393
CfoI GCGC 3 cut(s) 616, 2330, 3429
Cfr13I GGNCC 7 cut(s) 1987, 2987, 2988, 3044, 3269, 3270, 3330
CseI GACGC 1 cut(s) 1701
Csp6I GTAC 9 cut(s) 136, 389, 772, 1521, 1751, 1911, 1969, 2559, 2615
CviQI GTAC 9 cut(s) 136, 389, 772, 1521, 1751, 1911, 1969, 2559, 2615
DpnI GATC 5 cut(s) 308, 849, 1854, 2043, 2898
DpnII GATC 5 cut(s) 306, 847, 1852, 2041, 2896
DraI TTTAAA 1 cut(s) 430
DraIII CACNNNGTG 2 cut(s) 1979, 2125
EaeI YGGCCR 3 cut(s) 2197, 2257, 3253
Eam1104I CTCTTC 6 cut(s) 629, 934, 963, 1893, 2709, 3080
EarI CTCTTC 6 cut(s) 629, 934, 963, 1893, 2709, 3080
EciI GGCGGA 1 cut(s) 1196
Ecl136II GAGCTC 2 cut(s) 1879, 3121
Eco130I CCWWGG 9 cut(s) 266, 568, 817, 998, 1827, 2194, 2290, 2695, 3139
Eco24I GRGCYC 6 cut(s) 666, 1539, 1881, 2991, 3123, 3273
Eco31I GGTCTC 2 cut(s) 1176, 3377
Eco32I GATATC 4 cut(s) 931, 1311, 1421, 2184
Eco47I GGWCC 1 cut(s) 1987
Eco47III AGCGCT 1 cut(s) 3428
Eco53kI GAGCTC 2 cut(s) 1879, 3121
Eco57I CTGAAG 6 cut(s) 653, 1151, 1911, 2403, 2697, 2733
Eco88I CYCGRG 1 cut(s) 1547
Eco91I GGTNACC 1 cut(s) 1193
EcoICRI GAGCTC 2 cut(s) 1879, 3121
EcoNI CCTNNNNNAGG 1 cut(s) 2502
EcoO109I RGGNCCY 2 cut(s) 3269, 3270
EcoO65I GGTNACC 1 cut(s) 1193
EcoRII CCWGG 7 cut(s) 202, 1791, 2203, 2794, 3279, 3345, 3386
EcoRV GATATC 4 cut(s) 931, 1311, 1421, 2184
EcoT14I CCWWGG 9 cut(s) 266, 568, 817, 998, 1827, 2194, 2290, 2695, 3139
EcoT22I ATGCAT 2 cut(s) 1022, 3201
EcoT38I GRGCYC 6 cut(s) 666, 1539, 1881, 2991, 3123, 3273
ErhI CCWWGG 9 cut(s) 266, 568, 817, 998, 1827, 2194, 2290, 2695, 3139
Esp3I CGTCTC 2 cut(s) 1684, 3012
FalI AAGNNNNNCTT 4 cut(s) 509, 541, 1842, 1874
FaqI GGGAC 1 cut(s) 1934
FbaI TGATCA 1 cut(s) 2041
FblI GTMKAC 2 cut(s) 160, 2941
Fnu4HI GCNGC 6 cut(s) 695, 1739, 2165, 2814, 3068, 3225
FokI GGATG 2 cut(s) 1272, 1810
FriOI GRGCYC 6 cut(s) 666, 1539, 1881, 2991, 3123, 3273
Fsp4HI GCNGC 6 cut(s) 695, 1739, 2165, 2814, 3068, 3225
FspBI CTAG 7 cut(s) 267, 893, 1118, 2291, 2696, 2807, 3408
FspI TGCGCA 1 cut(s) 615
GlaI GCGC 3 cut(s) 615, 2329, 3428
GluI GCNGC 6 cut(s) 695, 1739, 2165, 2814, 3068, 3225
GsuI CTGGAG 2 cut(s) 456, 1240
HaeII RGCGCY 1 cut(s) 3430
HaeIII GGCC 9 cut(s) 111, 2164, 2199, 2259, 2989, 3046, 3255, 3271, 3332
HapII CCGG 4 cut(s) 1211, 1659, 3064, 3265
HgaI GACGC 1 cut(s) 1701
HhaI GCGC 3 cut(s) 616, 2330, 3429
Hin1I GRCGYC 1 cut(s) 1923
Hin6I GCGC 3 cut(s) 614, 2328, 3427
HinP1I GCGC 3 cut(s) 614, 2328, 3427
HincII GTYRAC 3 cut(s) 37, 2473, 2801
HindII GTYRAC 3 cut(s) 37, 2473, 2801
HindIII AAGCTT 1 cut(s) 19
HpaII CCGG 4 cut(s) 1211, 1659, 3064, 3265
HphI GGTGA 9 cut(s) 113, 526, 1121, 1484, 1673, 2132, 2537, 3044, 3052
HpyAV CCTTC 9 cut(s) 371, 616, 740, 1126, 1396, 1767, 2602, 2790, 2818
HpyCH4III ACNGT 7 cut(s) 557, 670, 1199, 1979, 2432, 2946, 3247
HpyCH4IV ACGT 8 cut(s) 318, 989, 1329, 1783, 1844, 1923, 2341, 3005
HpySE526I ACGT 8 cut(s) 318, 989, 1329, 1783, 1844, 1923, 2341, 3005
Hsp92I GRCGYC 1 cut(s) 1923
HspAI GCGC 3 cut(s) 614, 2328, 3427
Ksp22I TGATCA 1 cut(s) 2041
Kzo9I GATC 5 cut(s) 306, 847, 1852, 2041, 2896
Lsp1109I GCAGC 4 cut(s) 706, 1750, 2800, 3079
MaeI CTAG 7 cut(s) 267, 893, 1118, 2291, 2696, 2807, 3408
MaeII ACGT 8 cut(s) 318, 989, 1329, 1783, 1844, 1923, 2341, 3005
MaeIII GTNAC 8 cut(s) 119, 319, 466, 1193, 1240, 1669, 2128, 3182
MalI GATC 5 cut(s) 308, 849, 1854, 2043, 2898
MboI GATC 5 cut(s) 306, 847, 1852, 2041, 2896
MfeI CAATTG 2 cut(s) 66, 2268
MflI RGATCY 2 cut(s) 847, 1852
MhlI GDGCHC 9 cut(s) 666, 1539, 1881, 2289, 2364, 2991, 3123, 3273, 3407
MlsI TGGCCA 2 cut(s) 2199, 2259
MluNI TGGCCA 2 cut(s) 2199, 2259
MlyI GAGTC 3 cut(s) 177, 2717, 3179
Mox20I TGGCCA 2 cut(s) 2199, 2259
Mph1103I ATGCAT 2 cut(s) 1022, 3201
MroXI GAANNNNTTC 2 cut(s) 605, 1759
MscI TGGCCA 2 cut(s) 2199, 2259
MseI TTAA 9 cut(s) 150, 356, 429, 1800, 1818, 2009, 2253, 2660, 2763
MslI CAYNNNNRTG 2 cut(s) 1025, 2043
Msp20I TGGCCA 2 cut(s) 2199, 2259
MspCI CTTAAG 2 cut(s) 2008, 2659
MspI CCGG 4 cut(s) 1211, 1659, 3064, 3265
MunI CAATTG 2 cut(s) 66, 2268
Mva1269I GAATGC 5 cut(s) 235, 955, 1022, 1262, 2276
MvaI CCWGG 7 cut(s) 204, 1793, 2205, 2796, 3281, 3347, 3388
MvnI CGCG 1 cut(s) 2328
NciI CCSGG 3 cut(s) 1211, 3064, 3266
NcoI CCATGG 2 cut(s) 568, 3139
NdeII GATC 5 cut(s) 306, 847, 1852, 2041, 2896
NlaIV GGNNCC 5 cut(s) 1536, 2989, 3270, 3271, 3331
NmeAIII GCCGAG 1 cut(s) 256
NmuCI GTSAC 4 cut(s) 119, 466, 1669, 3182
NsbI TGCGCA 1 cut(s) 615
NsiI ATGCAT 2 cut(s) 1022, 3201
NspI RCATGY 8 cut(s) 275, 395, 1030, 1151, 2350, 2685, 2774, 3203
PaeI GCATGC 3 cut(s) 275, 2685, 3203
PciI ACATGT 1 cut(s) 391
PctI GAATGC 5 cut(s) 235, 955, 1022, 1262, 2276
PdmI GAANNNNTTC 2 cut(s) 605, 1759
PfeI GAWTC 9 cut(s) 62, 332, 1655, 1777, 2309, 2419, 2493, 2552, 3261
PflFI GACNNNGTC 1 cut(s) 3305
PflMI CCANNNNNTGG 1 cut(s) 2125
PkrI GCNGC 6 cut(s) 696, 1740, 2166, 2815, 3069, 3226
PleI GAGTC 3 cut(s) 176, 2716, 3179
PpsI GAGTC 3 cut(s) 176, 2716, 3179
PscI ACATGT 1 cut(s) 391
PshBI ATTAAT 1 cut(s) 2763
PsiI TTATAA 1 cut(s) 584
Psp124BI GAGCTC 2 cut(s) 1881, 3123
Psp1406I AACGTT 2 cut(s) 318, 1329
Psp6I CCWGG 7 cut(s) 202, 1791, 2203, 2794, 3279, 3345, 3386
PspEI GGTNACC 1 cut(s) 1193
PspGI CCWGG 7 cut(s) 202, 1791, 2203, 2794, 3279, 3345, 3386
PspN4I GGNNCC 5 cut(s) 1536, 2989, 3270, 3271, 3331
PspOMI GGGCCC 2 cut(s) 2987, 3269
PspPI GGNCC 7 cut(s) 1987, 2987, 2988, 3044, 3269, 3270, 3330
PstI CTGCAG 1 cut(s) 3398
PstNI CAGNNNCTG 1 cut(s) 3393
PsuI RGATCY 2 cut(s) 847, 1852
PsyI GACNNNGTC 1 cut(s) 3305
RsaI GTAC 9 cut(s) 137, 390, 773, 1522, 1752, 1912, 1970, 2560, 2616
RsaNI GTAC 9 cut(s) 136, 389, 772, 1521, 1751, 1911, 1969, 2559, 2615
RseI CAYNNNNRTG 2 cut(s) 1025, 2043
SacI GAGCTC 2 cut(s) 1881, 3123
SaqAI TTAA 9 cut(s) 150, 356, 429, 1800, 1818, 2009, 2253, 2660, 2763
SatI GCNGC 6 cut(s) 695, 1739, 2165, 2814, 3068, 3225
Sau3AI GATC 5 cut(s) 306, 847, 1852, 2041, 2896
Sau96I GGNCC 7 cut(s) 1987, 2987, 2988, 3044, 3269, 3270, 3330
SchI GAGTC 3 cut(s) 177, 2717, 3179
SduI GDGCHC 9 cut(s) 666, 1539, 1881, 2289, 2364, 2991, 3123, 3273, 3407
SfcI CTRYAG 3 cut(s) 1709, 3394, 3430
SinI GGWCC 1 cut(s) 1987
SmiMI CAYNNNNRTG 2 cut(s) 1025, 2043
SmlI CTYRAG 3 cut(s) 2008, 2659, 3374
SmoI CTYRAG 3 cut(s) 2008, 2659, 3374
SphI GCATGC 3 cut(s) 275, 2685, 3203
SsiI CCGC 6 cut(s) 241, 1207, 1424, 2165, 2326, 3224
SspMI CTAG 7 cut(s) 267, 893, 1118, 2291, 2696, 2807, 3408
SstI GAGCTC 2 cut(s) 1881, 3123
StyI CCWWGG 9 cut(s) 266, 568, 817, 998, 1827, 2194, 2290, 2695, 3139
TaaI ACNGT 7 cut(s) 557, 670, 1199, 1979, 2432, 2946, 3247
TaiI ACGT 8 cut(s) 321, 992, 1332, 1786, 1847, 1926, 2344, 3008
TaqI TCGA 3 cut(s) 1408, 1875, 3123
TatI WGTACW 6 cut(s) 388, 771, 1520, 1910, 1968, 2614
TauI GCSGC 2 cut(s) 2167, 3227
TfiI GAWTC 9 cut(s) 62, 332, 1655, 1777, 2309, 2419, 2493, 2552, 3261
Tru1I TTAA 9 cut(s) 150, 356, 429, 1800, 1818, 2009, 2253, 2660, 2763
Tru9I TTAA 9 cut(s) 150, 356, 429, 1800, 1818, 2009, 2253, 2660, 2763
TseFI GTSAC 4 cut(s) 119, 466, 1669, 3182
TseI GCWGC 4 cut(s) 694, 1738, 2813, 3067
Tsp45I GTSAC 4 cut(s) 119, 466, 1669, 3182
TspGWI ACGGA 2 cut(s) 488, 2383
Tth111I GACNNNGTC 1 cut(s) 3305
Van91I CCANNNNNTGG 1 cut(s) 2125
Vha464I CTTAAG 2 cut(s) 2008, 2659
VneI GTGCAC 1 cut(s) 2285
VpaK11BI GGWCC 1 cut(s) 1987
VspI ATTAAT 1 cut(s) 2763
XagI CCTNNNNNAGG 1 cut(s) 2502
XapI RAATTY 4 cut(s) 560, 1495, 1804, 2641
XbaI TCTAGA 1 cut(s) 1117
XceI RCATGY 8 cut(s) 275, 395, 1030, 1151, 2350, 2685, 2774, 3203
XcmI CCANNNNNNNNNTGG 1 cut(s) 1856
XmaJI CCTAGG 3 cut(s) 266, 2290, 2695
XmiI GTMKAC 2 cut(s) 160, 2941
XmnI GAANNNNTTC 2 cut(s) 605, 1759
XspI CTAG 7 cut(s) 267, 893, 1118, 2291, 2696, 2807, 3408
ZraI GACGTC 1 cut(s) 1924
Zsp2I ATGCAT 2 cut(s) 1022, 3201
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.