Rw0G001760

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00051
OG0002206
Physical Location & Seq
Forward (+)
28307 .. 34944
6638 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G001760.1

Sequence Viewer

Length: 3099 bp
ATGAGTAGTGAGGGGGAAAGCTTTTCCAATTGGGTTGACATGGATATTGATGATGATAATGAATCTGTTGATTTGACTAGCATACATAATCTGGGAGAAGCTTTTTTGAAGGATTTCTGTAAGGAGGCATCAGTTTCATTCTTCAACCAGTATGGCCTCATTAGTCACCAAATAAACTCGTACAATGACTTCATTAAAAATGGTATACAAAGAGTCTTTGATAGTTTTGATGAGATTATAGTTGAGCCTGGGTATGACCCATCAAAGAAGGGGAATGCAGAATGGCGATATGCTTCCATCAGGTTTGGGAAGGTTACCCTCATTCGACCAAGTTTTTGGGGCGGTGCTGTGAATGATAAGGAGTATAATATGCTACCTAGGCATGCTCGGCTTCAAAATATGACATACTCAGCCAAGATCAAAGTGAACGTTACTGTTGAGGTATATACTCAAAATCTTGTCAGAAGTGAAAAGTTTAAAACTGGGAAAGAACAATGTCTTGACAAGCAGATTCTCCCAGTGATGGTGAAGTCTGACTTGTGTTGGATGAAAGACGAGAAAGCTGACTGTGAATTTGACCATGGAGGTTATTTTATAATCAAGGGTGCAGAGAAGTCAGTGTACTTTATGTCAGCTGAAATCCCTCTCTGGATTTGGTTTTTTGCCCTTGGGGTTTATTCGGACAATGAAGTTACAGATCTGATTGATTATGGCAAAGAAGATGCAAGCATTTCAAGCATTCTAGTTGCCTCAATTCGTGATGCTGACCAACGAAAAGATAACTTCCGGAGAGGGAAGAATGCTCTCAGATATGTTGAAGAGATAATAAACAATAACACGTTTCCTCCTCGGGAAAGCATTGAAGAATGCATCAGCATGTATCTGTTCCCCAATCTCAGGGGCATGAAGCAAAAGGCTTGGTTTCTTGGCTATATGGTGAAGTCTCTTTTACAGACCTTCCGAGGCCACAGGAAATGTGACAACAGAGAAGAATTGAAGAACAAGAGGTTTCACTTGGCTGGTGAGCGTCTAGAGCGAGAGCTGAAGGCACACGTAGGACATGCAGGAAGGCGCATGGCAAAGGCTCTGCAGAGAGACCTTTGTGGTGACCGTGTTGTCCGCCTGGTTGAGCAATATCTAGATGCTACAGTAAGGATGTCTGGTGTGGTGGTAAATGTTGGGAAAACAATTCCACTGCAAACAATGGTAGATATGAGGAAGACACGCCTCCAAGTTCAATACACGGGGAAGGTTGGAGATGCCAGATATCCAAGCTTGTGGATGCAGGCATCCTTCTCACTTTGCTTTATCTCAACCCCAGATGGTGAGAATTGTGGGCTTGTGAAAAATTTGGCTACCACTGCACTGGTAAGTACAAACATATTGCAGCCCATATTGCCCGACTTTTTCTCTTGTGGCATGGAGGAGCTGGTAGATGATACTTCAACATCACTTCATGGGAAGAATAAAGTTTTTCTCAATGGGGATTGGGTTGGCGTGGAAATCAAAAGAGATGAGCAGCTCCTGGAAGTACGCATATATTCTGATGCTGGAAGGATTCTACATCCTTTCCTAGTAGTTAAGAATTTGAATAAGATTAAAGAATCAAAAGGGAAAAAACACACGTTCCAGGATCTTCTTGGCAATGGAATAATCGAGCTCATTGGAGCTGAAGAAGAAGAGGATTGCTGTACAGCATGGGACGTCAAGCATCTATTTATGGAGGGGAAGTCAGCAGTCAAGTACACACACTGTGAACTGGACCTATCATTTTTGTTAGGCTTAAGTTGTGGTATCATTCCGTTTTCAAACCATGATAATGCAAGGAGGGTTCTATACCAGTCTCAGAAGCACTCTCAACAGGCCATTGGTTTTTCTTCCACAAATCCTAACCAAAGAAGGCCGCTTTTTCGTACTATGATATCTAATTGCCTTGGCCAACCAGGAAACCCAGTGAGCCATAAGAGAGTTATGCCCAAGCCTGAATACTTTAATGGCCAAAATGCAATTGTTGCAGTCAATGTGCACCTAGGATATAACCAAGAAGATTCTATTGTGATGAACCGCGCAGCTGTGCAACGTGGTATGTTCAAATCTGAGCACATACGGAGCTACAAGGCTGAAATGACTATAAATTTTGGCAAAATACAGAGTAAATTTGGAAGTGTTGACAGCCTTGATGATGATGGATTCCCCTGTATAGGTGCATATCTGCAGAGTGGTGATACTATTATAGCAAGGTGCAGTGAATCTGGTACTAATCATAGCATCAAACTGAGGCATACTGAAAGAGGAAAGGTTCAGAAGGTTGTACTTTCCTCTAATGAAGAGGGAAAGAATTTTAGTGTTGTATCCTTAAGACAGCATGCATGGGCAGAAGGGTGTCCTAGGCTTTTGGAGTCTGAAGAGAACTTCCCCTTCACTATACAGGGGATAGTTCCAGATATTGTAATTAATCCACATGCATTTCCTTCACGACAAGCTCCTGGTCAACTCCTAGAGGCTGCTTTAGGGAAGGAGATTGCCTGTGGTGGCTCAATGAGATATGCAACTCCTTTCTCTATTCCCTCTGTTGATGACATCACAGATCAGCTTCATAGAGCTGGATTTCAAAGATGGGGAAATGAGAGAGTATACAGTGGTCAAACTGGTAAAATGGTTCGCCAACTGATATTTATGGGTCCAACGTTCTATCAACGTCTCATTCACATGTCTGAAGACAAAGTGAAGTTCAGGAACACTGGGCCAGTTCACCCTCTCACCCGGCAGCCAGTGGCAGACAGGACGAGATTTGGTGGGGTAAAGTTTGGTGAGATGGAGCGCGACTGCCTTATAGCCCATGGAGCATCAGCAAACTTACATGAACGCCTTTTCACTCTTAGTGACTCCTCCGAAATGCATGCTTGCCTGAAATGCAAAAATGCGGCTAATGTGATAGATGGAACAGTTGATGGCAGTAGAATCAGGGGTCCATATTGCCGGGTCTGTAAGTCTGTTGATGACATTGTCAGGTTGAATGTTCCATATGGGGCCAAGTTATTGTGCCAGGAACTTTTCAGCATGGGCATAAGTCTCAAGTTTGAGACCAGGCTCTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000228 GO:0000418 GO:0000419 GO:0000428 GO:0000785 GO:0000790 GO:0000792 GO:0002682 GO:0003674 GO:0003824 GO:0003899 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005720 GO:0005730 GO:0005736 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009605 GO:0009607 GO:0009620 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016020 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019222 GO:0019438 GO:0030422 GO:0030880 GO:0031047 GO:0031050 GO:0031974 GO:0031981 GO:0032259 GO:0032774 GO:0032991 GO:0034062 GO:0034641 GO:0034654 GO:0035194 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043331 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044451 GO:0044452 GO:0044454 GO:0044464 GO:0044728 GO:0046483 GO:0048519 GO:0048583 GO:0050776 GO:0050789 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0055029 GO:0060255 GO:0061695 GO:0065007 GO:0070013 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0097747 GO:0098542 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1901698 GO:1901699 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko01100 ko03020 ko05016 ko05169 map00230 map00240 map01100 map03020 map05016 map05169
Pfam Domains
Protein Families
RNA_pol_Rpb2_1 RNA_pol_Rpb2_2 RNA_pol_Rpb2_3 RNA_pol_Rpb2_4 RNA_pol_Rpb2_5 RNA_pol_Rpb2_6 RNA_pol_Rpb2_7

Protein Analysis

1032

Amino Acids

116.72

Weight (kDa)

8.12

Isoelectric Point (pI)

35.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNA_pol_Rpb2_1 PF04563 52 - 368 9.3e-25 RNA polymerase beta subunit
RNA_pol_Rpb2_2 PF04561 211 - 336 1.9e-07 RNA polymerase Rpb2, domain 2
RNA_pol_Rpb2_3 PF04565 398 - 456 4.4e-07 RNA polymerase Rpb2, domain 3
RNA_pol_Rpb2_5 PF04567 543 - 588 1.8e-07 RNA polymerase Rpb2, domain 5
RNA_pol_Rpb2_6 PF00562 597 - 930 1.2e-74 RNA polymerase Rpb2, domain 6
RNA_pol_Rpb2_7 PF04560 932 - 1028 2.3e-25 RNA polymerase Rpb2, domain 7
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 596
AasI GACNNNNNNGTC 1 cut(s) 1115
AatII GACGTC 1 cut(s) 1707
AccI GTMKAC 2 cut(s) 205, 2634
AccII CGCG 2 cut(s) 2067, 2823
AccIII TCCGGA 1 cut(s) 786
AciI CCGC 5 cut(s) 342, 1120, 1904, 2065, 2924
AclI AACGTT 2 cut(s) 429, 2687
AclWI GGATC 1 cut(s) 1641
AcoI YGGCCR 2 cut(s) 1936, 1996
AcsI RAATTY 6 cut(s) 572, 1348, 1585, 2134, 2156, 2338
AcuI CTGAAG 4 cut(s) 1064, 1692, 2424, 2736
AcyI GRCGYC 1 cut(s) 1704
AdeI CACNNNGTG 1 cut(s) 1754
AfaI GTAC 9 cut(s) 182, 623, 1375, 1533, 1693, 1745, 1915, 2257, 2313
AfiI CCNNNNNNNGG 3 cut(s) 523, 897, 2201
AflII CTTAAG 2 cut(s) 1783, 2356
AflIII ACRYGT 4 cut(s) 837, 1051, 1623, 2709
AjnI CCWGG 8 cut(s) 247, 1122, 1524, 1629, 1942, 2485, 3045, 3086
Alw21I GWGCWC 3 cut(s) 1662, 2028, 2103
Alw26I GTCTC 6 cut(s) 948, 1089, 1848, 2705, 3077, 3077
Alw44I GTGCAC 1 cut(s) 2024
AlwI GGATC 1 cut(s) 1641
AlwNI CAGNNNCTG 2 cut(s) 1525, 3093
Ama87I CYCGRG 1 cut(s) 849
Aor13HI TCCGGA 1 cut(s) 786
AoxI GGCC 8 cut(s) 154, 964, 1863, 1901, 1936, 1996, 2744, 3030
ApaLI GTGCAC 1 cut(s) 2024
ApeKI GCWGC 5 cut(s) 1387, 1519, 2069, 2504, 2767
ApoI RAATTY 6 cut(s) 572, 1348, 1585, 2134, 2156, 2338
ArsI GACNNNNNNTTYG 4 cut(s) 318, 350, 557, 589
AseI ATTAAT 1 cut(s) 2454
Asp700I GAANNNNTTC 1 cut(s) 113
AspA2I CCTAGG 3 cut(s) 377, 2029, 2387
AspLEI GCGC 3 cut(s) 1074, 2069, 2823
AspS9I GGNCC 5 cut(s) 1762, 2681, 2744, 2969, 3030
AsuC2I CCSGG 2 cut(s) 2764, 2981
AvaI CYCGRG 1 cut(s) 849
AvaII GGWCC 3 cut(s) 1762, 2681, 2969
AvrII CCTAGG 3 cut(s) 377, 2029, 2387
BaeGI GKGCMC 1 cut(s) 2028
BalI TGGCCA 2 cut(s) 1938, 1998
BanII GRGCYC 1 cut(s) 1662
BarI GAAGNNNNNNTAC 4 cut(s) 173, 205, 605, 637
BbsI GAAGAC 2 cut(s) 1226, 2724
Bbv12I GWGCWC 3 cut(s) 1662, 2028, 2103
BbvI GCAGC 5 cut(s) 1399, 1531, 2081, 2491, 2779
BccI CCATC 9 cut(s) 268, 305, 517, 1316, 2180, 2610, 2809, 2933, 2945
BcgI CGANNNNNNTGC 2 cut(s) 2882, 2916
BciT130I CCWGG 8 cut(s) 249, 1124, 1526, 1631, 1944, 2487, 3047, 3088
BciVI GTATCC 1 cut(s) 2362
BcnI CCSGG 2 cut(s) 2764, 2981
BcoDI GTCTC 6 cut(s) 948, 1089, 1848, 2705, 3077, 3077
BfaI CTAG 9 cut(s) 78, 378, 743, 1031, 1139, 1574, 2030, 2388, 2498
BfmI CTRYAG 3 cut(s) 1088, 1146, 2213
BfrI CTTAAG 2 cut(s) 1783, 2356
BfuI GTATCC 1 cut(s) 2362
BglII AGATCT 1 cut(s) 697
BisI GCNGC 7 cut(s) 1388, 1520, 1904, 2070, 2505, 2768, 2925
BlnI CCTAGG 3 cut(s) 377, 2029, 2387
BlsI GCNGC 7 cut(s) 1389, 1521, 1905, 2071, 2506, 2769, 2926
Bme18I GGWCC 3 cut(s) 1762, 2681, 2969
BmeT110I CYCGRG 1 cut(s) 849
BmgT120I GGNCC 5 cut(s) 1762, 2681, 2744, 2969, 3030
BmiI GGNNCC 3 cut(s) 2682, 2970, 3031
BmrI ACTGGG 4 cut(s) 492, 512, 1946, 2751
BmuI ACTGGG 4 cut(s) 492, 512, 1946, 2751
BpiI GAAGAC 2 cut(s) 1226, 2724
BpuEI CTTGAG 1 cut(s) 3059
BpuMI CCSGG 2 cut(s) 2764, 2981
BsaAI YACGTR 1 cut(s) 1054
BsaHI GRCGYC 1 cut(s) 1704
BsaI GGTCTC 2 cut(s) 1089, 3077
BsaWI WCCGGW 1 cut(s) 786
BsaXI ACNNNNNCTCC 4 cut(s) 829, 859, 1715, 1745
Bsc4I CCNNNNNNNGG 3 cut(s) 523, 897, 2201
Bse3DI GCAATG 1 cut(s) 1651
BseAI TCCGGA 1 cut(s) 786
BseBI CCWGG 8 cut(s) 249, 1124, 1526, 1631, 1944, 2487, 3047, 3088
BseGI GGATG 5 cut(s) 552, 1161, 1287, 1289, 1564
BseLI CCNNNNNNNGG 3 cut(s) 523, 897, 2201
BseMI GCAATG 1 cut(s) 1651
BseMII CTCAG 6 cut(s) 423, 820, 910, 1859, 2088, 2267
BseRI GAGGAG 3 cut(s) 837, 1439, 2878
BseSI GKGCMC 1 cut(s) 2028
BseXI GCAGC 5 cut(s) 1399, 1531, 2081, 2491, 2779
BsgI GTGCAG 3 cut(s) 627, 1347, 2263
Bsh1236I CGCG 2 cut(s) 2067, 2823
BshFI GGCC 8 cut(s) 156, 966, 1865, 1903, 1938, 1998, 2746, 3032
BsiHKAI GWGCWC 3 cut(s) 1662, 2028, 2103
BsiHKCI CYCGRG 1 cut(s) 849
BsiSI CCGG 3 cut(s) 787, 2764, 2980
BslFI GGGAC 1 cut(s) 1715
BslI CCNNNNNNNGG 3 cut(s) 523, 897, 2201
BsmAI GTCTC 6 cut(s) 948, 1089, 1848, 2705, 3077, 3077
BsmBI CGTCTC 1 cut(s) 2705
BsmFI GGGAC 1 cut(s) 1715
BsmI GAATGC 4 cut(s) 280, 738, 805, 872
BsnI GGCC 8 cut(s) 156, 966, 1865, 1903, 1938, 1998, 2746, 3032
Bso31I GGTCTC 2 cut(s) 1089, 3077
BsoBI CYCGRG 1 cut(s) 849
Bsp1286I GDGCHC 3 cut(s) 1662, 2028, 2103
Bsp13I TCCGGA 1 cut(s) 786
Bsp1407I TGTACA 1 cut(s) 1691
Bsp143I GATC 4 cut(s) 417, 697, 1633, 2587
Bsp19I CCATGG 2 cut(s) 580, 2839
BspACI CCGC 5 cut(s) 342, 1120, 1904, 2065, 2924
BspANI GGCC 8 cut(s) 156, 966, 1865, 1903, 1938, 1998, 2746, 3032
BspCNI CTCAG 6 cut(s) 422, 819, 909, 1858, 2089, 2268
BspEI TCCGGA 1 cut(s) 786
BspFNI CGCG 2 cut(s) 2067, 2823
BspLI GGNNCC 3 cut(s) 2682, 2970, 3031
BspMAI CTGCAG 2 cut(s) 1092, 2217
BspPI GGATC 1 cut(s) 1641
BspTI CTTAAG 2 cut(s) 1783, 2356
BspTNI GGTCTC 2 cut(s) 1089, 3077
BsrDI GCAATG 1 cut(s) 1651
BsrGI TGTACA 1 cut(s) 1691
BssMI GATC 4 cut(s) 417, 697, 1633, 2587
BssNAI GTATAC 2 cut(s) 206, 2635
BssNI GRCGYC 1 cut(s) 1704
BssT1I CCWWGG 7 cut(s) 377, 580, 667, 1933, 2029, 2387, 2839
Bst1107I GTATAC 2 cut(s) 206, 2635
Bst2UI CCWGG 8 cut(s) 249, 1124, 1526, 1631, 1944, 2487, 3047, 3088
Bst4CI ACNGT 7 cut(s) 436, 569, 1112, 1150, 1754, 2639, 2947
Bst6I CTCTTC 4 cut(s) 813, 1674, 2322, 2400
BstACI GRCGYC 1 cut(s) 1704
BstAFI CTTAAG 2 cut(s) 1783, 2356
BstAPI GCANNNNNTGC 1 cut(s) 2012
BstAUI TGTACA 1 cut(s) 1691
BstBAI YACGTR 1 cut(s) 1054
BstC8I GCNNGC 6 cut(s) 384, 727, 1287, 2367, 2901, 2905
BstDEI CTNAG 7 cut(s) 409, 806, 896, 1845, 2097, 2276, 2879
BstDSI CCRYGG 2 cut(s) 580, 2839
BstEII GGTNACC 2 cut(s) 313, 1106
BstF5I GGATG 5 cut(s) 552, 1161, 1287, 1289, 1564
BstFNI CGCG 2 cut(s) 2067, 2823
BstHHI GCGC 3 cut(s) 1074, 2069, 2823
BstKTI GATC 4 cut(s) 420, 700, 1636, 2590
BstMAI GTCTC 6 cut(s) 948, 1089, 1848, 2705, 3077, 3077
BstMBI GATC 4 cut(s) 417, 697, 1633, 2587
BstMWI GCNNNNNNNGC 9 cut(s) 379, 388, 735, 1033, 1361, 1396, 2012, 2843, 2913
BstNI CCWGG 8 cut(s) 249, 1124, 1526, 1631, 1944, 2487, 3047, 3088
BstNSI RCATGY 7 cut(s) 386, 880, 1064, 2369, 2465, 2713, 2903
BstPI GGTNACC 2 cut(s) 313, 1106
BstSFI CTRYAG 3 cut(s) 1088, 1146, 2213
BstSLI GKGCMC 1 cut(s) 2028
BstUI CGCG 2 cut(s) 2067, 2823
BstV1I GCAGC 5 cut(s) 1399, 1531, 2081, 2491, 2779
BstV2I GAAGAC 2 cut(s) 1226, 2724
BstX2I RGATCY 2 cut(s) 697, 1633
BstXI CCANNNNNNTGG 3 cut(s) 336, 1278, 1366
BstYI RGATCY 2 cut(s) 697, 1633
BstZ17I GTATAC 2 cut(s) 206, 2635
BsuI GTATCC 1 cut(s) 2362
BsuRI GGCC 8 cut(s) 156, 966, 1865, 1903, 1938, 1998, 2746, 3032
BtgI CCRYGG 2 cut(s) 580, 2839
BtsCI GGATG 5 cut(s) 552, 1161, 1287, 1289, 1564
BtsI GCAGTG 3 cut(s) 1193, 1359, 2251
Cac8I GCNNGC 6 cut(s) 384, 727, 1287, 2367, 2901, 2905
CaiI CAGNNNCTG 2 cut(s) 1525, 3093
CfoI GCGC 3 cut(s) 1074, 2069, 2823
Cfr13I GGNCC 5 cut(s) 1762, 2681, 2744, 2969, 3030
CseI GACGC 1 cut(s) 1016
Csp6I GTAC 9 cut(s) 181, 622, 1374, 1532, 1692, 1744, 1914, 2256, 2312
CviQI GTAC 9 cut(s) 181, 622, 1374, 1532, 1692, 1744, 1914, 2256, 2312
DdeI CTNAG 7 cut(s) 409, 806, 896, 1845, 2097, 2276, 2879
DpnI GATC 4 cut(s) 419, 699, 1635, 2589
DpnII GATC 4 cut(s) 417, 697, 1633, 2587
DraI TTTAAA 1 cut(s) 478
DraIII CACNNNGTG 1 cut(s) 1754
DrdI GACNNNNNNGTC 1 cut(s) 1115
DseDI GACNNNNNNGTC 1 cut(s) 1115
EaeI YGGCCR 2 cut(s) 1936, 1996
Eam1104I CTCTTC 4 cut(s) 813, 1674, 2322, 2400
EarI CTCTTC 4 cut(s) 813, 1674, 2322, 2400
EciI GGCGGA 1 cut(s) 1109
Ecl136II GAGCTC 1 cut(s) 1660
Eco130I CCWWGG 7 cut(s) 377, 580, 667, 1933, 2029, 2387, 2839
Eco24I GRGCYC 1 cut(s) 1662
Eco31I GGTCTC 2 cut(s) 1089, 3077
Eco32I GATATC 2 cut(s) 1268, 1923
Eco47I GGWCC 3 cut(s) 1762, 2681, 2969
Eco53kI GAGCTC 1 cut(s) 1660
Eco57I CTGAAG 4 cut(s) 1064, 1692, 2424, 2736
Eco88I CYCGRG 1 cut(s) 849
Eco91I GGTNACC 2 cut(s) 313, 1106
EcoICRI GAGCTC 1 cut(s) 1660
EcoO65I GGTNACC 2 cut(s) 313, 1106
EcoRII CCWGG 8 cut(s) 247, 1122, 1524, 1629, 1942, 2485, 3045, 3086
EcoRV GATATC 2 cut(s) 1268, 1923
EcoT14I CCWWGG 7 cut(s) 377, 580, 667, 1933, 2029, 2387, 2839
EcoT22I ATGCAT 4 cut(s) 872, 2371, 2467, 2901
EcoT38I GRGCYC 1 cut(s) 1662
ErhI CCWWGG 7 cut(s) 377, 580, 667, 1933, 2029, 2387, 2839
Esp3I CGTCTC 1 cut(s) 2705
FalI AAGNNNNNCTT 2 cut(s) 521, 553
FaqI GGGAC 1 cut(s) 1715
FauNDI CATATG 1 cut(s) 3025
FblI GTMKAC 2 cut(s) 205, 2634
Fnu4HI GCNGC 7 cut(s) 1388, 1520, 1904, 2070, 2505, 2768, 2925
FokI GGATG 5 cut(s) 559, 1168, 1276, 1294, 1551
FriOI GRGCYC 1 cut(s) 1662
Fsp4HI GCNGC 7 cut(s) 1388, 1520, 1904, 2070, 2505, 2768, 2925
FspBI CTAG 9 cut(s) 78, 378, 743, 1031, 1139, 1574, 2030, 2388, 2498
GlaI GCGC 3 cut(s) 1073, 2068, 2822
GluI GCNGC 7 cut(s) 1388, 1520, 1904, 2070, 2505, 2768, 2925
HaeIII GGCC 8 cut(s) 156, 966, 1865, 1903, 1938, 1998, 2746, 3032
HapII CCGG 3 cut(s) 787, 2764, 2980
HgaI GACGC 1 cut(s) 1016
HhaI GCGC 3 cut(s) 1074, 2069, 2823
Hin1I GRCGYC 1 cut(s) 1704
Hin6I GCGC 3 cut(s) 1072, 2067, 2821
HinP1I GCGC 3 cut(s) 1072, 2067, 2821
HincII GTYRAC 3 cut(s) 37, 2170, 2492
HindII GTYRAC 3 cut(s) 37, 2170, 2492
HindIII AAGCTT 3 cut(s) 19, 99, 1273
HpaII CCGG 3 cut(s) 787, 2764, 2980
HpyCH4III ACNGT 7 cut(s) 436, 569, 1112, 1150, 1754, 2639, 2947
HpyCH4IV ACGT 8 cut(s) 429, 839, 1053, 1625, 1704, 2080, 2687, 2698
HpyF10VI GCNNNNNNNGC 9 cut(s) 379, 388, 735, 1033, 1361, 1396, 2012, 2843, 2913
HpyF3I CTNAG 7 cut(s) 409, 806, 896, 1845, 2097, 2276, 2879
HpySE526I ACGT 8 cut(s) 429, 839, 1053, 1625, 1704, 2080, 2687, 2698
Hsp92I GRCGYC 1 cut(s) 1704
HspAI GCGC 3 cut(s) 1072, 2067, 2821
Kpn2I TCCGGA 1 cut(s) 786
Kzo9I GATC 4 cut(s) 417, 697, 1633, 2587
LmnI GCTCC 7 cut(s) 1426, 1527, 1667, 2109, 2488, 2818, 2843
Lsp1109I GCAGC 5 cut(s) 1399, 1531, 2081, 2491, 2779
MaeI CTAG 9 cut(s) 78, 378, 743, 1031, 1139, 1574, 2030, 2388, 2498
MaeII ACGT 8 cut(s) 429, 839, 1053, 1625, 1704, 2080, 2687, 2698
MaeIII GTNAC 7 cut(s) 164, 313, 430, 691, 977, 1106, 2882
MalI GATC 4 cut(s) 419, 699, 1635, 2589
MboI GATC 4 cut(s) 417, 697, 1633, 2587
MfeI CAATTG 2 cut(s) 28, 2007
MflI RGATCY 2 cut(s) 697, 1633
MhlI GDGCHC 3 cut(s) 1662, 2028, 2103
MlsI TGGCCA 2 cut(s) 1938, 1998
MluNI TGGCCA 2 cut(s) 1938, 1998
MlyI GAGTC 3 cut(s) 222, 2408, 2879
MmeI TCCRAC 3 cut(s) 524, 1234, 2708
Mox20I TGGCCA 2 cut(s) 1938, 1998
Mph1103I ATGCAT 4 cut(s) 872, 2371, 2467, 2901
MroI TCCGGA 1 cut(s) 786
MroXI GAANNNNTTC 1 cut(s) 113
MscI TGGCCA 2 cut(s) 1938, 1998
MseI TTAA 8 cut(s) 195, 477, 1581, 1599, 1784, 1992, 2357, 2454
MslI CAYNNNNRTG 3 cut(s) 875, 1818, 2708
Msp20I TGGCCA 2 cut(s) 1938, 1998
MspA1I CMGCKG 2 cut(s) 635, 2072
MspCI CTTAAG 2 cut(s) 1783, 2356
MspI CCGG 3 cut(s) 787, 2764, 2980
MunI CAATTG 2 cut(s) 28, 2007
Mva1269I GAATGC 4 cut(s) 280, 738, 805, 872
MvaI CCWGG 8 cut(s) 249, 1124, 1526, 1631, 1944, 2487, 3047, 3088
MvnI CGCG 2 cut(s) 2067, 2823
MwoI GCNNNNNNNGC 9 cut(s) 379, 388, 735, 1033, 1361, 1396, 2012, 2843, 2913
NciI CCSGG 2 cut(s) 2764, 2981
NcoI CCATGG 2 cut(s) 580, 2839
NdeI CATATG 1 cut(s) 3025
NdeII GATC 4 cut(s) 417, 697, 1633, 2587
NlaIV GGNNCC 3 cut(s) 2682, 2970, 3031
NmeAIII GCCGAG 1 cut(s) 367
NmuCI GTSAC 4 cut(s) 164, 977, 1106, 2882
NsiI ATGCAT 4 cut(s) 872, 2371, 2467, 2901
NspI RCATGY 7 cut(s) 386, 880, 1064, 2369, 2465, 2713, 2903
PaeI GCATGC 3 cut(s) 386, 2369, 2903
PciI ACATGT 1 cut(s) 2709
PctI GAATGC 4 cut(s) 280, 738, 805, 872
PdmI GAANNNNTTC 1 cut(s) 113
PfeI GAWTC 8 cut(s) 62, 511, 1558, 1604, 2048, 2190, 2249, 2961
PflFI GACNNNGTC 1 cut(s) 3005
PfoI TCCNGGA 2 cut(s) 1524, 1629
PkrI GCNGC 7 cut(s) 1389, 1521, 1905, 2071, 2506, 2769, 2926
PleI GAGTC 3 cut(s) 221, 2407, 2879
PpsI GAGTC 3 cut(s) 221, 2407, 2879
Ppu21I YACGTR 1 cut(s) 1054
PscI ACATGT 1 cut(s) 2709
PshBI ATTAAT 1 cut(s) 2454
PsiI TTATAA 1 cut(s) 596
Psp124BI GAGCTC 1 cut(s) 1662
Psp1406I AACGTT 2 cut(s) 429, 2687
Psp6I CCWGG 8 cut(s) 247, 1122, 1524, 1629, 1942, 2485, 3045, 3086
PspEI GGTNACC 2 cut(s) 313, 1106
PspGI CCWGG 8 cut(s) 247, 1122, 1524, 1629, 1942, 2485, 3045, 3086
PspN4I GGNNCC 3 cut(s) 2682, 2970, 3031
PspPI GGNCC 5 cut(s) 1762, 2681, 2744, 2969, 3030
PstI CTGCAG 2 cut(s) 1092, 2217
PstNI CAGNNNCTG 2 cut(s) 1525, 3093
PsuI RGATCY 2 cut(s) 697, 1633
PsyI GACNNNGTC 1 cut(s) 3005
PvuII CAGCTG 2 cut(s) 635, 2072
RsaI GTAC 9 cut(s) 182, 623, 1375, 1533, 1693, 1745, 1915, 2257, 2313
RsaNI GTAC 9 cut(s) 181, 622, 1374, 1532, 1692, 1744, 1914, 2256, 2312
RseI CAYNNNNRTG 3 cut(s) 875, 1818, 2708
SacI GAGCTC 1 cut(s) 1662
SaqAI TTAA 8 cut(s) 195, 477, 1581, 1599, 1784, 1992, 2357, 2454
SatI GCNGC 7 cut(s) 1388, 1520, 1904, 2070, 2505, 2768, 2925
Sau3AI GATC 4 cut(s) 417, 697, 1633, 2587
Sau96I GGNCC 5 cut(s) 1762, 2681, 2744, 2969, 3030
SchI GAGTC 3 cut(s) 222, 2408, 2879
SduI GDGCHC 3 cut(s) 1662, 2028, 2103
SfcI CTRYAG 3 cut(s) 1088, 1146, 2213
SinI GGWCC 3 cut(s) 1762, 2681, 2969
SmiMI CAYNNNNRTG 3 cut(s) 875, 1818, 2708
SmlI CTYRAG 3 cut(s) 1783, 2356, 3074
SmoI CTYRAG 3 cut(s) 1783, 2356, 3074
SphI GCATGC 3 cut(s) 386, 2369, 2903
SsiI CCGC 5 cut(s) 342, 1120, 1904, 2065, 2924
SspMI CTAG 9 cut(s) 78, 378, 743, 1031, 1139, 1574, 2030, 2388, 2498
SstI GAGCTC 1 cut(s) 1662
StyI CCWWGG 7 cut(s) 377, 580, 667, 1933, 2029, 2387, 2839
TaaI ACNGT 7 cut(s) 436, 569, 1112, 1150, 1754, 2639, 2947
TaiI ACGT 8 cut(s) 432, 842, 1056, 1628, 1707, 2083, 2690, 2701
TaqI TCGA 2 cut(s) 325, 1656
TatI WGTACW 5 cut(s) 621, 1373, 1691, 1743, 2311
TauI GCSGC 2 cut(s) 1906, 2927
TfiI GAWTC 8 cut(s) 62, 511, 1558, 1604, 2048, 2190, 2249, 2961
Tru1I TTAA 8 cut(s) 195, 477, 1581, 1599, 1784, 1992, 2357, 2454
Tru9I TTAA 8 cut(s) 195, 477, 1581, 1599, 1784, 1992, 2357, 2454
TseFI GTSAC 4 cut(s) 164, 977, 1106, 2882
TseI GCWGC 5 cut(s) 1387, 1519, 2069, 2504, 2767
Tsp45I GTSAC 4 cut(s) 164, 977, 1106, 2882
TspGWI ACGGA 2 cut(s) 1791, 2122
Tth111I GACNNNGTC 1 cut(s) 3005
Vha464I CTTAAG 2 cut(s) 1783, 2356
VneI GTGCAC 1 cut(s) 2024
VpaK11BI GGWCC 3 cut(s) 1762, 2681, 2969
VspI ATTAAT 1 cut(s) 2454
XapI RAATTY 6 cut(s) 572, 1348, 1585, 2134, 2156, 2338
XbaI TCTAGA 2 cut(s) 1030, 1138
XceI RCATGY 7 cut(s) 386, 880, 1064, 2369, 2465, 2713, 2903
XcmI CCANNNNNNNNNTGG 1 cut(s) 1637
XmaJI CCTAGG 3 cut(s) 377, 2029, 2387
XmiI GTMKAC 2 cut(s) 205, 2634
XmnI GAANNNNTTC 1 cut(s) 113
XspI CTAG 9 cut(s) 78, 378, 743, 1031, 1139, 1574, 2030, 2388, 2498
ZraI GACGTC 1 cut(s) 1705
Zsp2I ATGCAT 4 cut(s) 872, 2371, 2467, 2901
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.