FvH4_1g21420

Belongs to the glycosyl hydrolase 5 (cellulase A) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0002687
Physical Location & Seq
Forward (+)
13358720 .. 13359951
1232 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g21420.t1

Sequence Viewer

Length: 816 bp
ATGTCGTCCAAAGGCTTGACTGCTGTGAGGATCCCAGTTGGATGGTGGATTGCATATGATCCAACACCTCCAAAGCCTTTTGTTGGAGGATCATTGCAAGCCTTGGACAATGCTTTCTCTTGGGCACAAAAATATGGGTTGAAGGTGATTATAGATCTACATGCAGTTCAAGGTTCCCAAAATCGTATGGATCACAGTGCAACAAGAGATGGGTATCTGGAATTTGGAGATTCCAACATACAGGACACCGTGAAAGTTATTGATTTCCTAGCAGCAAGATATGCTAAAAATCTAGCTCTCGCAGCAATTGAGTTGATAAATGAGCCTTGGGCACCACTTGTGACTCTGGACTTCCTAGAAAAGTATTACAAAGCTGGTTATGATGCAGTAAGGAAGTACACTTCAAGTGCCTATGTGATCCTATCAAATCGCTTGGGACCTGCCGATCCAAAGGAGCTTATATCCTTTGCCCAAGGCCTCAGTAAGGTAGTCATAGACGTGCATTACTACAGCATGTATTTGGACATGTTCAATGGCCTTAGTGTGCAACAAAACATTGATTTCATCTACCACCAACGAGGTTCTGATCTCGCTGCTGTGACCACTACAAATGGACCCCTGACGTTTGTTGGGGAATGGACTGCAGAATGGGCAATAAATGGAGCATCTATGCAAGACTACCAGAGATTCGCCAAAGCACAACTAGATGTCTACGGCCGTGCTAGTTTTGGATGGGCATACTGGTCTTATAAGTGTCAGTACAATCACTGGAGCCTCAAATGGATGATTGAGAATAACTATATAAAACTCAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cellulase

Protein Analysis

272

Amino Acids

30.72

Weight (kDa)

6.51

Isoelectric Point (pI)

21.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cellulase PF00150 2 - 251 3.8e-19 Cellulase (glycosyl hydrolase family 5)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 750
Acc36I ACCTGC 1 cut(s) 448
AccB1I GGYRCC 1 cut(s) 331
AccI GTMKAC 1 cut(s) 711
AclWI GGATC 7 cut(s) 25, 38, 53, 97, 198, 412, 440
AcoI YGGCCR 1 cut(s) 715
AcsI RAATTY 1 cut(s) 221
AfaI GTAC 2 cut(s) 398, 761
AfiI CCNNNNNNNGG 2 cut(s) 83, 484
AflIII ACRYGT 1 cut(s) 525
AgsI TTSAA 4 cut(s) 142, 170, 405, 532
AjiI CACGTC 1 cut(s) 499
AluBI AGCT 3 cut(s) 296, 374, 457
AluI AGCT 3 cut(s) 296, 374, 457
AlwI GGATC 7 cut(s) 25, 38, 53, 97, 198, 412, 440
AoxI GGCC 3 cut(s) 475, 535, 715
ApeKI GCWGC 3 cut(s) 272, 302, 593
ApoI RAATTY 1 cut(s) 221
AspS9I GGNCC 2 cut(s) 437, 614
AsuHPI GGTGA 1 cut(s) 157
AvaII GGWCC 2 cut(s) 437, 614
BaeGI GKGCMC 2 cut(s) 127, 334
BamHI GGATCC 1 cut(s) 30
BanI GGYRCC 1 cut(s) 331
BbvI GCAGC 3 cut(s) 284, 314, 580
BccI CCATC 3 cut(s) 36, 203, 726
BceAI ACGGC 2 cut(s) 702, 730
BfaI CTAG 5 cut(s) 269, 293, 356, 704, 723
BfmI CTRYAG 2 cut(s) 508, 642
BfuAI ACCTGC 1 cut(s) 448
BglII AGATCT 1 cut(s) 154
BisI GCNGC 3 cut(s) 273, 303, 594
BlsI GCNGC 3 cut(s) 274, 304, 595
Bme18I GGWCC 2 cut(s) 437, 614
BmgBI CACGTC 1 cut(s) 499
BmgT120I GGNCC 2 cut(s) 437, 614
BmiI GGNNCC 6 cut(s) 32, 175, 333, 438, 616, 773
BmrI ACTGGG 1 cut(s) 29
BmsI GCATC 2 cut(s) 373, 674
BmuI ACTGGG 1 cut(s) 29
BpmI CTGGAG 1 cut(s) 790
BsaBI GATNNNNATC 1 cut(s) 213
BsaJI CCNNGG 3 cut(s) 102, 326, 472
Bsc4I CCNNNNNNNGG 2 cut(s) 83, 484
Bse1I ACTGG 3 cut(s) 35, 746, 773
Bse3DI GCAATG 1 cut(s) 92
Bse8I GATNNNNATC 1 cut(s) 213
BseDI CCNNGG 3 cut(s) 102, 326, 472
BseGI GGATG 3 cut(s) 47, 737, 789
BseJI GATNNNNATC 1 cut(s) 213
BseLI CCNNNNNNNGG 2 cut(s) 83, 484
BseMI GCAATG 1 cut(s) 92
BseMII CTCAG 1 cut(s) 493
BseNI ACTGG 3 cut(s) 35, 746, 773
BseSI GKGCMC 2 cut(s) 127, 334
BseX3I CGGCCG 1 cut(s) 715
BseXI GCAGC 3 cut(s) 284, 314, 580
Bsh1285I CGRYCG 1 cut(s) 718
BshFI GGCC 3 cut(s) 477, 537, 717
BshNI GGYRCC 1 cut(s) 331
BsiEI CGRYCG 1 cut(s) 718
BslFI GGGAC 1 cut(s) 450
BslI CCNNNNNNNGG 2 cut(s) 83, 484
BsmFI GGGAC 1 cut(s) 450
BsnI GGCC 3 cut(s) 477, 537, 717
Bsp1286I GDGCHC 2 cut(s) 127, 334
Bsp143I GATC 8 cut(s) 30, 58, 89, 154, 190, 417, 445, 586
BspANI GGCC 3 cut(s) 477, 537, 717
BspCNI CTCAG 1 cut(s) 492
BspLI GGNNCC 6 cut(s) 32, 175, 333, 438, 616, 773
BspMAI CTGCAG 1 cut(s) 646
BspMI ACCTGC 1 cut(s) 448
BspPI GGATC 7 cut(s) 25, 38, 53, 97, 198, 412, 440
BspT107I GGYRCC 1 cut(s) 331
BsrDI GCAATG 1 cut(s) 92
BsrI ACTGG 3 cut(s) 35, 746, 773
BssECI CCNNGG 3 cut(s) 102, 326, 472
BssMI GATC 8 cut(s) 30, 58, 89, 154, 190, 417, 445, 586
BssT1I CCWWGG 3 cut(s) 102, 326, 472
Bst4CI ACNGT 2 cut(s) 197, 250
BstAPI GCANNNNNTGC 1 cut(s) 281
BstC8I GCNNGC 1 cut(s) 99
BstDEI CTNAG 2 cut(s) 479, 539
BstENI CCTNNNNNAGG 1 cut(s) 482
BstF5I GGATG 3 cut(s) 47, 737, 789
BstKTI GATC 8 cut(s) 33, 61, 92, 157, 193, 420, 448, 589
BstMBI GATC 8 cut(s) 30, 58, 89, 154, 190, 417, 445, 586
BstMCI CGRYCG 1 cut(s) 718
BstMWI GCNNNNNNNGC 3 cut(s) 281, 302, 650
BstNSI RCATGY 3 cut(s) 164, 517, 529
BstSFI CTRYAG 2 cut(s) 508, 642
BstSLI GKGCMC 2 cut(s) 127, 334
BstV1I GCAGC 3 cut(s) 284, 314, 580
BstX2I RGATCY 2 cut(s) 30, 154
BstXI CCANNNNNNTGG 1 cut(s) 42
BstYI RGATCY 2 cut(s) 30, 154
BstZI CGGCCG 1 cut(s) 715
BsuRI GGCC 3 cut(s) 477, 537, 717
BtrI CACGTC 1 cut(s) 499
BtsCI GGATG 3 cut(s) 47, 737, 789
BtsIMutI CAGTG 2 cut(s) 202, 766
BveI ACCTGC 1 cut(s) 448
Cac8I GCNNGC 1 cut(s) 99
Cfr13I GGNCC 2 cut(s) 437, 614
Csp6I GTAC 2 cut(s) 397, 760
CviAII CATG 3 cut(s) 161, 514, 526
CviQI GTAC 2 cut(s) 397, 760
DdeI CTNAG 2 cut(s) 479, 539
DpnI GATC 8 cut(s) 32, 60, 91, 156, 192, 419, 447, 588
DpnII GATC 8 cut(s) 30, 58, 89, 154, 190, 417, 445, 586
EaeI YGGCCR 1 cut(s) 715
EagI CGGCCG 1 cut(s) 715
EclXI CGGCCG 1 cut(s) 715
Eco130I CCWWGG 3 cut(s) 102, 326, 472
Eco147I AGGCCT 1 cut(s) 477
Eco47I GGWCC 2 cut(s) 437, 614
Eco52I CGGCCG 1 cut(s) 715
EcoNI CCTNNNNNAGG 1 cut(s) 482
EcoO109I RGGNCCY 1 cut(s) 437
EcoT14I CCWWGG 3 cut(s) 102, 326, 472
ErhI CCWWGG 3 cut(s) 102, 326, 472
FaeI CATG 3 cut(s) 164, 517, 529
FaqI GGGAC 1 cut(s) 450
FatI CATG 3 cut(s) 160, 513, 525
FauNDI CATATG 1 cut(s) 55
FblI GTMKAC 1 cut(s) 711
Fnu4HI GCNGC 3 cut(s) 273, 303, 594
FokI GGATG 3 cut(s) 54, 744, 796
Fsp4HI GCNGC 3 cut(s) 273, 303, 594
FspBI CTAG 5 cut(s) 269, 293, 356, 704, 723
GluI GCNGC 3 cut(s) 273, 303, 594
GsuI CTGGAG 1 cut(s) 790
HaeIII GGCC 3 cut(s) 477, 537, 717
Hin1II CATG 3 cut(s) 164, 517, 529
HinfI GANTC 3 cut(s) 230, 343, 687
HphI GGTGA 1 cut(s) 157
Hpy166II GTNNAC 2 cut(s) 399, 712
Hpy188I TCNGA 1 cut(s) 586
Hpy188III TCNNGA 2 cut(s) 218, 347
Hpy8I GTNNAC 2 cut(s) 399, 712
HpyAV CCTTC 1 cut(s) 136
HpyCH4III ACNGT 2 cut(s) 197, 250
HpyCH4IV ACGT 2 cut(s) 498, 623
HpyCH4V TGCA 9 cut(s) 53, 97, 164, 200, 386, 502, 547, 644, 673
HpyF10VI GCNNNNNNNGC 3 cut(s) 281, 302, 650
HpyF3I CTNAG 2 cut(s) 479, 539
HpySE526I ACGT 2 cut(s) 498, 623
Hsp92II CATG 3 cut(s) 164, 517, 529
Kzo9I GATC 8 cut(s) 30, 58, 89, 154, 190, 417, 445, 586
LmnI GCTCC 3 cut(s) 454, 662, 771
Lsp1109I GCAGC 3 cut(s) 284, 314, 580
LweI GCATC 2 cut(s) 373, 674
MaeI CTAG 5 cut(s) 269, 293, 356, 704, 723
MaeII ACGT 2 cut(s) 498, 623
MaeIII GTNAC 2 cut(s) 340, 598
MalI GATC 8 cut(s) 32, 60, 91, 156, 192, 419, 447, 588
MboI GATC 8 cut(s) 30, 58, 89, 154, 190, 417, 445, 586
MfeI CAATTG 1 cut(s) 306
MflI RGATCY 2 cut(s) 30, 154
MhlI GDGCHC 2 cut(s) 127, 334
MluCI AATT 3 cut(s) 221, 306, 811
MlyI GAGTC 1 cut(s) 337
MmeI TCCRAC 4 cut(s) 19, 64, 86, 258
MnlI CCTC 6 cut(s) 21, 78, 80, 488, 572, 785
MslI CAYNNNNRTG 1 cut(s) 497
MunI CAATTG 1 cut(s) 306
MwoI GCNNNNNNNGC 3 cut(s) 281, 302, 650
NdeI CATATG 1 cut(s) 55
NdeII GATC 8 cut(s) 30, 58, 89, 154, 190, 417, 445, 586
NlaIII CATG 3 cut(s) 164, 517, 529
NlaIV GGNNCC 6 cut(s) 32, 175, 333, 438, 616, 773
NmuCI GTSAC 2 cut(s) 340, 598
NspI RCATGY 3 cut(s) 164, 517, 529
PceI AGGCCT 1 cut(s) 477
PciI ACATGT 1 cut(s) 525
PfeI GAWTC 2 cut(s) 230, 687
PkrI GCNGC 3 cut(s) 274, 304, 595
PleI GAGTC 1 cut(s) 337
PpsI GAGTC 1 cut(s) 337
PpuMI RGGWCCY 1 cut(s) 437
PscI ACATGT 1 cut(s) 525
PsiI TTATAA 1 cut(s) 750
Psp5II RGGWCCY 1 cut(s) 437
PspN4I GGNNCC 6 cut(s) 32, 175, 333, 438, 616, 773
PspPI GGNCC 2 cut(s) 437, 614
PspPPI RGGWCCY 1 cut(s) 437
PstI CTGCAG 1 cut(s) 646
PsuI RGATCY 2 cut(s) 30, 154
RsaI GTAC 2 cut(s) 398, 761
RsaNI GTAC 2 cut(s) 397, 760
RseI CAYNNNNRTG 1 cut(s) 497
SatI GCNGC 3 cut(s) 273, 303, 594
Sau3AI GATC 8 cut(s) 30, 58, 89, 154, 190, 417, 445, 586
Sau96I GGNCC 2 cut(s) 437, 614
SchI GAGTC 1 cut(s) 337
SduI GDGCHC 2 cut(s) 127, 334
SfaNI GCATC 2 cut(s) 373, 674
SfcI CTRYAG 2 cut(s) 508, 642
SinI GGWCC 2 cut(s) 437, 614
SmiMI CAYNNNNRTG 1 cut(s) 497
Sse9I AATT 3 cut(s) 221, 306, 811
SseBI AGGCCT 1 cut(s) 477
SspMI CTAG 5 cut(s) 269, 293, 356, 704, 723
StuI AGGCCT 1 cut(s) 477
StyI CCWWGG 3 cut(s) 102, 326, 472
TaaI ACNGT 2 cut(s) 197, 250
TaiI ACGT 2 cut(s) 501, 626
TasI AATT 3 cut(s) 221, 306, 811
TatI WGTACW 2 cut(s) 396, 759
TfiI GAWTC 2 cut(s) 230, 687
TscAI CASTG 2 cut(s) 202, 773
TseFI GTSAC 2 cut(s) 340, 598
TseI GCWGC 3 cut(s) 272, 302, 593
Tsp45I GTSAC 2 cut(s) 340, 598
TspDTI ATGAA 1 cut(s) 553
TspRI CASTG 2 cut(s) 202, 773
VpaK11BI GGWCC 2 cut(s) 437, 614
XagI CCTNNNNNAGG 1 cut(s) 482
XapI RAATTY 1 cut(s) 221
XceI RCATGY 3 cut(s) 164, 517, 529
XcmI CCANNNNNNNNNTGG 1 cut(s) 42
XmiI GTMKAC 1 cut(s) 711
XspI CTAG 5 cut(s) 269, 293, 356, 704, 723
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.