Rroxscaffold_2G00130710

Belongs to the glycosyl hydrolase 5 (cellulase A) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0002687
Physical Location & Seq
Forward (+)
66965517 .. 66970178
4662 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00130710.1

Sequence Viewer

Length: 1683 bp
ATGGCATACTATAATATCTACCCTGTTTTGGCTCTTTACTTGTCATGTTTTGCTTCTCTTTCATCAGCGCAGAACTTGAAGTTGCCATTAAAATCTGTGAATCTTGGGAACTGGCTTGTTGTAGAGGGTTGGATGAAACCTTCTCTCTTTTATGAAATACCTAACAAAGATCTTTTGGATGGGACAAAGGTGCAATTGAAGTCCACAAAGCTTCAGAACTATTTGGCTGCAGAAAATGGGGGTAATTCCATTGTTGTTATTAATCGCACTGCAGCCTCTGATTGGGAAACTTTCAGGTTGTGGAGGATCAATGAAAATTCTTTCAACTTTCGAGTATCAAACAAGCAGTTTGTGGGATTGTCACGCCAGGGAACCAACAGGATAGTAGCAATGAGAGATGCACCTGGTGATACGGAAACATTTCGGATTGTAAGAAAAAAGGGCGAGCCAAATAGGATTCGGATTCAAGCATCCAATGGCTTGTTTCTCCAGGCAAAATCAAATACGGTACTGACTGCAGATTATGGAGGATCGGGTTGGGATGACAGTAATCCATCTGTGTTTATTATGACAATAGTAGGCTCATTACAAGGTGAATACCAAATAACAAATGGTTATGGTCCGGATAAAGCCCGTCAAGTCCTGCAGGACCACCGGAACTCTTACGTCACTGAAGAGGATTTCAGATTCATGTCATCCAACGGCTTGAATGCTGTGAGGATACCAGTTGGATGGTGGATTGCATATGATCCAACACCTCCAAAGCCTTTTGTTGGGGGATCATTGCAAGCCTTGGACAATGCTTTCACTTGGGCTCGAAAATATGGGTTGAAGGTGATTATAGATCTACATGCAGTTCAAGCTTCCCAAAACGGCATGGAACACACTCTCGGAGCAATTGAGTTAATAAATGAGCCTTGGGCACCACTTGTGACTCTAGACACCCTCAAAAAGTATTACAAAGCTGGTTATGATGCAGTAAGGAAGCACACCTCAAGTGCTCATGTGATCCTATCAAACCGCTTGGGACCTGCTGATCCAAAGGAGCTCCTCTCATTTGCCCAAGGCCTCAGTAAGGTAGTCATAGATGTGCATTACTACAGCTTGTATTCAGACATGTTCAACGGTTTTAGTGTGCAACAAAACATCAATTTCATATACAACCAACGAGGTTCTGATCTCGCTGCTGTGACCACTTCTAATGGACCCCTCACTTTTGTTGGGGAATGGGTTGCAGAATGGGCAATAAATGGAGCATCTATGGAAGACTACCAGAGATTTGCCAAAGCACAACTAGCTGTTTACGGCCGTGCTACTTTTGGATGGGCATACTGGTCTTACAAGTCGCAGAACTTGAAGTTGCCATTAAAATCTGTGAATCTTGGGAACTGGCTTGTTGTAGAGGGTTGGATGAAACCTTCTCTATTTGATGAAATACCTAACAAAGATCTTTTGGATGGGACAAAGGTGCAATTGAAGTCCCCAAAGCTTCAGAACTATTTGGCTGCAGAAAATGGGGGTAATTCCATTGTTGTTATTAATCGCACTGCAGCCTCTGGTTGGGAAACTTTCAGGGTGAGCTGTCCAGATCAGTGTTGTGGAGGATCGATGAAAATTCTTTCAACTTTCGAGTATCAAACAAGCAGTTTGTGGGATTGTCACGCCAGGGAACCAACAGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cellulase

Protein Analysis

560

Amino Acids

62.6

Weight (kDa)

8.78

Isoelectric Point (pI)

25.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7910 PF25490 55 - 193 1.2e-63 Domain of unknown function (DUF7910)
Cellulase PF00150 220 - 286 1.1e-06 Cellulase (glycosyl hydrolase family 5)
Cellulase PF00150 299 - 450 2.6e-06 Cellulase (glycosyl hydrolase family 5)
DUF7910 PF25490 481 - 526 4.1e-17 Domain of unknown function (DUF7910)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1039
AccB1I GGYRCC 1 cut(s) 922
AccIII TCCGGA 1 cut(s) 622
AciI CCGC 1 cut(s) 1021
AclWI GGATC 7 cut(s) 314, 538, 743, 787, 1003, 1031, 1612
AcoI YGGCCR 1 cut(s) 1306
AcsI RAATTY 2 cut(s) 316, 1614
AcuI CTGAAG 3 cut(s) 197, 693, 1475
AdeI CACNNNGTG 1 cut(s) 407
AfaI GTAC 1 cut(s) 510
AfiI CCNNNNNNNGG 5 cut(s) 28, 282, 773, 1075, 1560
AflIII ACRYGT 1 cut(s) 1116
AjnI CCWGG 4 cut(s) 366, 403, 489, 1664
AluBI AGCT 8 cut(s) 211, 863, 965, 1048, 1104, 1298, 1489, 1581
AluI AGCT 8 cut(s) 211, 863, 965, 1048, 1104, 1298, 1489, 1581
Alw21I GWGCWC 2 cut(s) 1003, 1050
AlwI GGATC 7 cut(s) 314, 538, 743, 787, 1003, 1031, 1612
AlwNI CAGNNNCTG 2 cut(s) 278, 1556
Aor13HI TCCGGA 1 cut(s) 622
AoxI GGCC 2 cut(s) 1066, 1306
ApeKI GCWGC 5 cut(s) 227, 272, 1184, 1505, 1550
ApoI RAATTY 2 cut(s) 316, 1614
AseI ATTAAT 2 cut(s) 261, 1539
Asp700I GAANNNNTTC 1 cut(s) 420
AspLEI GCGC 1 cut(s) 70
AspS9I GGNCC 4 cut(s) 620, 649, 1028, 1205
AsuHPI GGTGA 4 cut(s) 419, 605, 847, 1588
AvaII GGWCC 4 cut(s) 620, 649, 1028, 1205
BaeGI GKGCMC 1 cut(s) 925
BanI GGYRCC 1 cut(s) 922
BanII GRGCYC 2 cut(s) 817, 1050
BbsI GAAGAC 1 cut(s) 1272
Bbv12I GWGCWC 2 cut(s) 1003, 1050
BbvI GCAGC 5 cut(s) 214, 284, 1171, 1492, 1562
BccI CCATC 5 cut(s) 173, 562, 726, 1317, 1451
BceAI ACGGC 4 cut(s) 718, 889, 1293, 1321
BciT130I CCWGG 4 cut(s) 368, 405, 491, 1666
BciVI GTATCC 1 cut(s) 714
BfaI CTAG 2 cut(s) 938, 1295
BfmI CTRYAG 7 cut(s) 228, 270, 516, 644, 1099, 1506, 1548
BfuAI ACCTGC 1 cut(s) 1039
BfuI GTATCC 1 cut(s) 714
BglII AGATCT 3 cut(s) 169, 844, 1447
BisI GCNGC 5 cut(s) 228, 273, 1185, 1506, 1551
BlsI GCNGC 5 cut(s) 229, 274, 1186, 1507, 1552
Bme1390I CCNGG 4 cut(s) 368, 405, 491, 1666
Bme18I GGWCC 4 cut(s) 620, 649, 1028, 1205
BmgT120I GGNCC 4 cut(s) 620, 649, 1028, 1205
BmiI GGNNCC 5 cut(s) 373, 924, 1029, 1207, 1671
BmrFI CCNGG 4 cut(s) 368, 405, 491, 1666
BmsI GCATC 4 cut(s) 388, 479, 964, 1265
BpiI GAAGAC 1 cut(s) 1272
BplI GAGNNNNNCTC 2 cut(s) 1037, 1069
BpmI CTGGAG 1 cut(s) 473
BpuEI CTTGAG 1 cut(s) 979
Bsa29I ATCGAT 1 cut(s) 1607
BsaJI CCNNGG 5 cut(s) 367, 792, 917, 1063, 1665
BsaWI WCCGGW 2 cut(s) 622, 654
Bsc4I CCNNNNNNNGG 5 cut(s) 28, 282, 773, 1075, 1560
Bse1I ACTGG 4 cut(s) 116, 725, 1337, 1394
Bse3DI GCAATG 2 cut(s) 396, 782
BseAI TCCGGA 1 cut(s) 622
BseBI CCWGG 4 cut(s) 368, 405, 491, 1666
BseCI ATCGAT 1 cut(s) 1607
BseDI CCNNGG 5 cut(s) 367, 792, 917, 1063, 1665
BseGI GGATG 9 cut(s) 138, 184, 470, 547, 695, 737, 1328, 1416, 1462
BseLI CCNNNNNNNGG 5 cut(s) 28, 282, 773, 1075, 1560
BseMI GCAATG 2 cut(s) 396, 782
BseMII CTCAG 1 cut(s) 1084
BseNI ACTGG 4 cut(s) 116, 725, 1337, 1394
BseRI GAGGAG 1 cut(s) 1040
BseSI GKGCMC 1 cut(s) 925
BseX3I CGGCCG 1 cut(s) 1306
BseXI GCAGC 5 cut(s) 214, 284, 1171, 1492, 1562
Bsh1285I CGRYCG 1 cut(s) 1309
BshFI GGCC 2 cut(s) 1068, 1308
BshNI GGYRCC 1 cut(s) 922
BshVI ATCGAT 1 cut(s) 1607
BsiEI CGRYCG 1 cut(s) 1309
BsiHKAI GWGCWC 2 cut(s) 1003, 1050
BsiSI CCGG 2 cut(s) 623, 655
BslFI GGGAC 4 cut(s) 196, 1041, 1465, 1474
BslI CCNNNNNNNGG 5 cut(s) 28, 282, 773, 1075, 1560
BsmFI GGGAC 4 cut(s) 196, 1041, 1465, 1474
BsmI GAATGC 1 cut(s) 715
BsnI GGCC 2 cut(s) 1068, 1308
Bsp1286I GDGCHC 4 cut(s) 817, 925, 1003, 1050
Bsp13I TCCGGA 1 cut(s) 622
BspACI CCGC 1 cut(s) 1021
BspANI GGCC 2 cut(s) 1068, 1308
BspCNI CTCAG 1 cut(s) 1083
BspDI ATCGAT 1 cut(s) 1607
BspEI TCCGGA 1 cut(s) 622
BspLI GGNNCC 5 cut(s) 373, 924, 1029, 1207, 1671
BspMAI CTGCAG 6 cut(s) 232, 274, 520, 648, 1510, 1552
BspMI ACCTGC 1 cut(s) 1039
BspPI GGATC 7 cut(s) 314, 538, 743, 787, 1003, 1031, 1612
BspT107I GGYRCC 1 cut(s) 922
BsrDI GCAATG 2 cut(s) 396, 782
BsrI ACTGG 4 cut(s) 116, 725, 1337, 1394
BssECI CCNNGG 5 cut(s) 367, 792, 917, 1063, 1665
BssT1I CCWWGG 3 cut(s) 792, 917, 1063
Bst2UI CCWGG 4 cut(s) 368, 405, 491, 1666
Bst4CI ACNGT 3 cut(s) 508, 548, 1127
Bst6I CTCTTC 1 cut(s) 669
BstC8I GCNNGC 2 cut(s) 446, 789
BstDEI CTNAG 1 cut(s) 1070
BstENI CCTNNNNNAGG 1 cut(s) 1073
BstF5I GGATG 9 cut(s) 138, 184, 470, 547, 695, 737, 1328, 1416, 1462
BstHHI GCGC 1 cut(s) 70
BstMCI CGRYCG 1 cut(s) 1309
BstMWI GCNNNNNNNGC 3 cut(s) 860, 1241, 1295
BstNI CCWGG 4 cut(s) 368, 405, 491, 1666
BstNSI RCATGY 2 cut(s) 854, 1120
BstSCI CCNGG 4 cut(s) 366, 403, 489, 1664
BstSFI CTRYAG 7 cut(s) 228, 270, 516, 644, 1099, 1506, 1548
BstSLI GKGCMC 1 cut(s) 925
BstV1I GCAGC 5 cut(s) 214, 284, 1171, 1492, 1562
BstV2I GAAGAC 1 cut(s) 1272
BstX2I RGATCY 3 cut(s) 169, 844, 1447
BstXI CCANNNNNNTGG 1 cut(s) 732
BstYI RGATCY 3 cut(s) 169, 844, 1447
BstZI CGGCCG 1 cut(s) 1306
Bsu15I ATCGAT 1 cut(s) 1607
BsuI GTATCC 1 cut(s) 714
BsuRI GGCC 2 cut(s) 1068, 1308
BsuTUI ATCGAT 1 cut(s) 1607
BtsCI GGATG 9 cut(s) 138, 184, 470, 547, 695, 737, 1328, 1416, 1462
BtsI GCAGTG 2 cut(s) 267, 1545
BtsIMutI CAGTG 4 cut(s) 267, 669, 1545, 1598
BveI ACCTGC 1 cut(s) 1039
Cac8I GCNNGC 2 cut(s) 446, 789
CaiI CAGNNNCTG 2 cut(s) 278, 1556
CfoI GCGC 1 cut(s) 70
Cfr13I GGNCC 4 cut(s) 620, 649, 1028, 1205
ClaI ATCGAT 1 cut(s) 1607
CsiI ACCWGGT 1 cut(s) 403
Csp6I GTAC 1 cut(s) 509
CviAII CATG 6 cut(s) 45, 691, 851, 877, 1004, 1117
CviQI GTAC 1 cut(s) 509
DdeI CTNAG 1 cut(s) 1070
DraIII CACNNNGTG 1 cut(s) 407
EaeI YGGCCR 1 cut(s) 1306
EagI CGGCCG 1 cut(s) 1306
Eam1104I CTCTTC 1 cut(s) 669
EarI CTCTTC 1 cut(s) 669
Ecl136II GAGCTC 1 cut(s) 1048
EclXI CGGCCG 1 cut(s) 1306
Eco130I CCWWGG 3 cut(s) 792, 917, 1063
Eco147I AGGCCT 1 cut(s) 1068
Eco24I GRGCYC 2 cut(s) 817, 1050
Eco47I GGWCC 4 cut(s) 620, 649, 1028, 1205
Eco52I CGGCCG 1 cut(s) 1306
Eco53kI GAGCTC 1 cut(s) 1048
Eco57I CTGAAG 3 cut(s) 197, 693, 1475
EcoICRI GAGCTC 1 cut(s) 1048
EcoNI CCTNNNNNAGG 1 cut(s) 1073
EcoO109I RGGNCCY 1 cut(s) 1028
EcoRII CCWGG 4 cut(s) 366, 403, 489, 1664
EcoT14I CCWWGG 3 cut(s) 792, 917, 1063
EcoT38I GRGCYC 2 cut(s) 817, 1050
ErhI CCWWGG 3 cut(s) 792, 917, 1063
FaeI CATG 6 cut(s) 48, 694, 854, 880, 1007, 1120
FaqI GGGAC 4 cut(s) 196, 1041, 1465, 1474
FatI CATG 6 cut(s) 44, 690, 850, 876, 1003, 1116
FauNDI CATATG 1 cut(s) 745
Fnu4HI GCNGC 5 cut(s) 228, 273, 1185, 1506, 1551
FokI GGATG 9 cut(s) 145, 191, 457, 554, 682, 744, 1335, 1423, 1469
FriOI GRGCYC 2 cut(s) 817, 1050
Fsp4HI GCNGC 5 cut(s) 228, 273, 1185, 1506, 1551
FspBI CTAG 2 cut(s) 938, 1295
GlaI GCGC 1 cut(s) 69
GluI GCNGC 5 cut(s) 228, 273, 1185, 1506, 1551
GsuI CTGGAG 1 cut(s) 473
HaeIII GGCC 2 cut(s) 1068, 1308
HapII CCGG 2 cut(s) 623, 655
HhaI GCGC 1 cut(s) 70
Hin1II CATG 6 cut(s) 48, 694, 854, 880, 1007, 1120
Hin6I GCGC 1 cut(s) 68
HinP1I GCGC 1 cut(s) 68
HindIII AAGCTT 3 cut(s) 209, 861, 1487
HinfI GANTC 6 cut(s) 100, 457, 463, 687, 934, 1378
HpaII CCGG 2 cut(s) 623, 655
HphI GGTGA 4 cut(s) 419, 605, 847, 1588
Hpy166II GTNNAC 2 cut(s) 204, 1303
Hpy188I TCNGA 9 cut(s) 216, 280, 426, 462, 686, 893, 1114, 1177, 1494
Hpy188III TCNNGA 3 cut(s) 623, 938, 1586
Hpy8I GTNNAC 2 cut(s) 204, 1303
HpyAV CCTTC 3 cut(s) 150, 826, 1428
HpyCH4III ACNGT 3 cut(s) 508, 548, 1127
HpyCH4IV ACGT 1 cut(s) 666
HpyF10VI GCNNNNNNNGC 3 cut(s) 860, 1241, 1295
HpyF3I CTNAG 1 cut(s) 1070
HpySE526I ACGT 1 cut(s) 666
Hsp92II CATG 6 cut(s) 48, 694, 854, 880, 1007, 1120
HspAI GCGC 1 cut(s) 68
Kpn2I TCCGGA 1 cut(s) 622
LmnI GCTCC 4 cut(s) 893, 1045, 1053, 1253
Lsp1109I GCAGC 5 cut(s) 214, 284, 1171, 1492, 1562
LweI GCATC 4 cut(s) 388, 479, 964, 1265
MabI ACCWGGT 1 cut(s) 403
MaeI CTAG 2 cut(s) 938, 1295
MaeII ACGT 1 cut(s) 666
MaeIII GTNAC 5 cut(s) 360, 667, 931, 1189, 1658
MboII GAAGA 2 cut(s) 686, 1277
MfeI CAATTG 3 cut(s) 194, 897, 1472
MflI RGATCY 3 cut(s) 169, 844, 1447
MhlI GDGCHC 4 cut(s) 817, 925, 1003, 1050
MluCI AATT 8 cut(s) 194, 244, 316, 897, 1150, 1472, 1522, 1614
MlyI GAGTC 1 cut(s) 928
MmeI TCCRAC 5 cut(s) 110, 709, 723, 776, 1388
MroI TCCGGA 1 cut(s) 622
MroXI GAANNNNTTC 1 cut(s) 420
MseI TTAA 5 cut(s) 89, 261, 905, 1367, 1539
MslI CAYNNNNRTG 1 cut(s) 1088
MspI CCGG 2 cut(s) 623, 655
MspR9I CCNGG 4 cut(s) 368, 405, 491, 1666
MunI CAATTG 3 cut(s) 194, 897, 1472
Mva1269I GAATGC 1 cut(s) 715
MvaI CCWGG 4 cut(s) 368, 405, 491, 1666
MwoI GCNNNNNNNGC 3 cut(s) 860, 1241, 1295
NdeI CATATG 1 cut(s) 745
NlaIII CATG 6 cut(s) 48, 694, 854, 880, 1007, 1120
NlaIV GGNNCC 5 cut(s) 373, 924, 1029, 1207, 1671
NmuCI GTSAC 5 cut(s) 360, 667, 931, 1189, 1658
NspI RCATGY 2 cut(s) 854, 1120
PceI AGGCCT 1 cut(s) 1068
PciI ACATGT 1 cut(s) 1116
PctI GAATGC 1 cut(s) 715
PdmI GAANNNNTTC 1 cut(s) 420
PfeI GAWTC 5 cut(s) 100, 457, 463, 687, 1378
PkrI GCNGC 5 cut(s) 229, 274, 1186, 1507, 1552
PleI GAGTC 1 cut(s) 928
PpsI GAGTC 1 cut(s) 928
PpuMI RGGWCCY 1 cut(s) 1028
PscI ACATGT 1 cut(s) 1116
PshBI ATTAAT 2 cut(s) 261, 1539
Psp124BI GAGCTC 1 cut(s) 1050
Psp5II RGGWCCY 1 cut(s) 1028
Psp6I CCWGG 4 cut(s) 366, 403, 489, 1664
PspGI CCWGG 4 cut(s) 366, 403, 489, 1664
PspN4I GGNNCC 5 cut(s) 373, 924, 1029, 1207, 1671
PspPI GGNCC 4 cut(s) 620, 649, 1028, 1205
PspPPI RGGWCCY 1 cut(s) 1028
PstI CTGCAG 6 cut(s) 232, 274, 520, 648, 1510, 1552
PstNI CAGNNNCTG 2 cut(s) 278, 1556
PsuI RGATCY 3 cut(s) 169, 844, 1447
RsaI GTAC 1 cut(s) 510
RsaNI GTAC 1 cut(s) 509
RseI CAYNNNNRTG 1 cut(s) 1088
SacI GAGCTC 1 cut(s) 1050
SaqAI TTAA 5 cut(s) 89, 261, 905, 1367, 1539
SatI GCNGC 5 cut(s) 228, 273, 1185, 1506, 1551
Sau96I GGNCC 4 cut(s) 620, 649, 1028, 1205
SbfI CCTGCAGG 1 cut(s) 648
SchI GAGTC 1 cut(s) 928
ScrFI CCNGG 4 cut(s) 368, 405, 491, 1666
SdaI CCTGCAGG 1 cut(s) 648
SduI GDGCHC 4 cut(s) 817, 925, 1003, 1050
SexAI ACCWGGT 1 cut(s) 403
SfaNI GCATC 4 cut(s) 388, 479, 964, 1265
SfcI CTRYAG 7 cut(s) 228, 270, 516, 644, 1099, 1506, 1548
SinI GGWCC 4 cut(s) 620, 649, 1028, 1205
SmiMI CAYNNNNRTG 1 cut(s) 1088
SmlI CTYRAG 1 cut(s) 994
SmoI CTYRAG 1 cut(s) 994
Sse8387I CCTGCAGG 1 cut(s) 648
Sse9I AATT 8 cut(s) 194, 244, 316, 897, 1150, 1472, 1522, 1614
SseBI AGGCCT 1 cut(s) 1068
SsiI CCGC 1 cut(s) 1021
SspMI CTAG 2 cut(s) 938, 1295
SstI GAGCTC 1 cut(s) 1050
StuI AGGCCT 1 cut(s) 1068
StyD4I CCNGG 4 cut(s) 366, 403, 489, 1664
StyI CCWWGG 3 cut(s) 792, 917, 1063
TaaI ACNGT 3 cut(s) 508, 548, 1127
TaiI ACGT 1 cut(s) 669
TaqI TCGA 4 cut(s) 331, 817, 1607, 1629
TasI AATT 8 cut(s) 194, 244, 316, 897, 1150, 1472, 1522, 1614
TfiI GAWTC 5 cut(s) 100, 457, 463, 687, 1378
Tru1I TTAA 5 cut(s) 89, 261, 905, 1367, 1539
Tru9I TTAA 5 cut(s) 89, 261, 905, 1367, 1539
TscAI CASTG 4 cut(s) 274, 676, 1552, 1598
TseFI GTSAC 5 cut(s) 360, 667, 931, 1189, 1658
TseI GCWGC 5 cut(s) 227, 272, 1184, 1505, 1550
Tsp45I GTSAC 5 cut(s) 360, 667, 931, 1189, 1658
TspDTI ATGAA 9 cut(s) 51, 149, 168, 327, 679, 1144, 1427, 1446, 1625
TspGWI ACGGA 1 cut(s) 428
TspRI CASTG 4 cut(s) 274, 676, 1552, 1598
VpaK11BI GGWCC 4 cut(s) 620, 649, 1028, 1205
VspI ATTAAT 2 cut(s) 261, 1539
XagI CCTNNNNNAGG 1 cut(s) 1073
XapI RAATTY 2 cut(s) 316, 1614
XbaI TCTAGA 1 cut(s) 937
XceI RCATGY 2 cut(s) 854, 1120
XcmI CCANNNNNNNNNTGG 2 cut(s) 608, 732
XmnI GAANNNNTTC 1 cut(s) 420
XspI CTAG 2 cut(s) 938, 1295
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.