Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000007

Orthogroup ID

OG0002687

Physical Location & Seq

Genomic Coordinates Reverse (-)

36235480 .. 36244338

Gene Structure 8859 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug07G0331000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2556 bp

ATGCAGAACAAGGTTTGTAGCTGGAAATTAGGCCAAAATGGGCAGCAGGTGATTAAAGGTCGTCATGAAGCATCAGATGATGATCAACCACCTCATGATCAACATCACAATTACAAGAATAATGATCAAATGATGTACTCAAACATATCATCGTCGTCGGATCTCAACTTGAGAAACCTCTCAAAGCTTATACTTCCTCCTTTGGGTGTCTCGAGCTACAACCAGCCGACTCAAATGCACTCAAAAGGCTGGATTATTTTACCCATGGACTCCAGATACAGGAGTTGGGAGACATTTATGGTGCTTTTGGTGGCTTACTCAGCCTGGGTTTACCCGTTTGAGGTTGCTTTTCTCATGTCGTCGTCGGGAAAAAGCAGCAAGCCTCTCTACTTAGCAGACTACATTGTTGATCTATTTTTCGGCATCGATATCATATTGACATTCTTCGTCGCATATATTGATCCCAGAACTCAATTACTTGTTCTCGACTCCAAAAAGATCGCTACAAGATATCTTTCGACATGGTTTGTTATGGATTTGGCATCAACCCTCCCCTTTGAGATTATAGGCAAACACAAACTCGGTCTCTCCTACTCTCTCTTGGGATTGCTCCGATTTTGGCGATTGAGACGAGCAAAGCAACTCTTCACAAGGCTTGAGAAGGACATCAGGTTTAGCTACTTCTGGGTCAGATGTGCCAGACTTTTATCTGTGACACTGTTTACAGTACATTGTGCTGGGTGCCTGTACTACCTGCTAGCAGACCGATACCCGCACCAAGGGAGGACATGGATTGGAACTGTTATTCCCAATTTCAGAGAGACGAGTCTGTGGATCCGCTACATTTCAGCCCTCTACTGGTCCATCACCACCATGTCCACCGTTGGTTATGGTGACCTCCATGCAGTGAACACCATGGAAATGATCTTCATCATTTTATACATGCTTTTCAACCTCGGCCTTACTGCTTACCTAATTGGCAACATGACAAACTTAGTTGTTGAAGGCACTCGCCGTACCATGGAATTTAGAAACAGCGTTGAAGCAGCATCAAACTTTGTTGACCGCAATCACTTGCCTCCAAGGTTGAAGAATCAAATTCTGGCCTATATTTGCTTGAGATTTAAGGCTGAGAGCTTGAACCAGCAGCAAATAATTGAACAGCTCCCCAAGTCAATTTGTAAAAGCATCTGTCAACATTTGTTCCTGCCAACAGTAATGAAAGTTTATCTTTTCAAGGGTGTCTCAAGGGAAATACTTCTAATTCTGGTTGCAAACATGAAGGCAGAGTACATACCACCAAGAGAAGATGTTGTAATGCAAAACGAAGCACCCGATGATATATACATCATTGTTTCTGGAGAAGTGGAAATCATTGATTGTGAGATGGATAAAGAGCTAGTCGCCGGGACTTTGAAATCTGGGGACATGTTTGGAGAAGTAGGTGCACTTTGTTGTAGGCCTCAAAGCTTTACATATCGGACAAAAACACTTTCACAATTCTTGAGACTCAAAACCACTGCACTAAAAGAAGCAATGCAGACCAAACAAGAAGACAATGCTCAAATGCTTAAGAATTTCAAGCAGCATCACAAAAAGCTCAGGGATCTGAGAATTGGAGAAATATTGGCTGAGAGTGGGGAAGATGAGGCCATGGGTGATCCAAGTTTAGCATTCGACTTATTGACTATTGCCAGCAAAGGGAATGCTGCACTTCTTGAAGAGCTCCTCAAGTCAAAGTTGGATCCTGACATTGGAGACTCCAAAGGAAGAACACCTCTGCATGTAGCAGCATCAAACGGGCATGAAGAATGTGTCTTGGTACTCCTCAAGTATGCTTGTAACATACATGTAAGAGACACTAATGGTAACACGGCAATATGGGAAGCAATTGCAGCAAAGCACCATTCCATATTCAAGATCCTCTACTATTATGCTGCCTTTTCTTATCCCTACACTGCCGGTGATCTTTTATGCACAGCTGCCAGAAGAAACGATCTGACTGTGATGAAGGAACTGTTGAAGCATGAATTGCATGTTGACTCAAAGGATTTCGACGGAAAAACAGCAGTACAAGTAGCCATGGAAGAAAACAATGTAGACATGGTAAACTTGCTGGTGATGAATGGAGCAGATGTCACCAATGTTTCACATGACTACTTATTTCCTTCACAAACCTTAAGCGATATGCTGCAAAAACGAGAGGTTGGACACCGGATTACAGTGCCAGACCCTACCCTGAATGAAGTGCTTTTGTTCAGAAATGATGGAGAGAAAAATGTTGATTGGAAAAAATCCAATGGAGTGAATTTTCCAAGAGTGAGCATATATAGAGGCCATCCTGTGACGAGGAGAAACACCTGTTGCACTGAAGCCGGGAGGTTGATTAGATTGCCCCATTCACTAGAGGAGCTCAAAAATATTGCAGGTGAAAAGTTTGGGTTTGATCCAGAGAATGTAATATTAACGGATGAAGAAGGAGCAGAAATAGACTGTATCGAAGTGATTAGAGATAATGACAAGCTTTTCATATATATAGTGGAGGACACAAATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0003674 GO:0003824 GO:0004338 GO:0004553 GO:0005575 GO:0005576 GO:0005623 GO:0005975 GO:0005976 GO:0006073 GO:0008150 GO:0008152 GO:0008422 GO:0009056 GO:0009057 GO:0009251 GO:0009986 GO:0009987 GO:0015926 GO:0016052 GO:0016787 GO:0016798 GO:0043170 GO:0044042 GO:0044237 GO:0044238 GO:0044260 GO:0044262 GO:0044264 GO:0044464 GO:0071704 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cellulase

Protein Analysis

851

Amino Acids

97.23

Weight (kDa)

6.58

Isoelectric Point (pI)

31.37

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ion_trans	PF00520	93 - 340	4.5e-42	Ion transport protein
Ion_trans_2	PF07885	264 - 334	3.6e-13	Ion channel
cNMP_binding	PF00027	431 - 515	6.4e-13	Cyclic nucleotide-binding domain
Ank_4	PF13637	563 - 610	1e-06	Ankyrin repeats (many copies)
Ank_2	PF12796	564 - 645	5.8e-15	Ankyrin repeats (3 copies)
Ank_5	PF13857	581 - 627	1.2e-08	Ankyrin repeats (many copies)
Ank	PF00023	590 - 620	8.8e-08	Ankyrin repeat
Ank_2	PF12796	657 - 714	4.9e-08	Ankyrin repeats (3 copies)
KHA	PF11834	773 - 844	3.9e-22	KHA, dimerisation domain of potassium ion channel

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Orthologous Genes (Group: OG0002687)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g21360 FvH4_1g21360 FvH4_1g21420 FvH4_5g37950
malus_domestica	MD01G1025400.v1.1 MD08G1178600.v1.1
prunus_persica	Prupe.1G512300_v2.0.a1 Prupe.6G181100_v2.0.a1
pyrus_communis	pycom01g05350 pycom08g15290 pycom08g15300
rosa_chinensis	RchiOBHm_Chr2g0115211 RchiOBHm_Chr7g0239891
rosa_laevigata	RLG00000000720 RLG00000018166
rosa_multiflora	Rmu_co8458197.1_g000001 Rmu_sc0000540.1_g000053 Rmu_sc0002784.1_g000034 Rmu_sc0005738.1_g000014 Rmu_sc0032904.1_g000001
rosa_roxburghii	Rroxscaffold_2G00130710 Rroxscaffold_2G00130730 Rroxscaffold_3G00220700
rosa_rugosa	Rorug02G0194700 Rorug02G0194800 Rorug02G0194800 Rorug02G0194900 Rorug07G0331000
rosa_samantha	Rh2AG252900 Rh2AG253000 Rh2BG262600 Rh2CG257600 Rh2DG261100 Rh2DG261200 Rh7AG484400 Rh7BG457600 Rh7CG501900 Rh7DG470100
rosa_wichuraiana	Rw2G019750 Rw7G040640

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	37, 2417
Acc36I	ACCTGC	3 cut(s)	37, 762, 2417
AccB1I	GGYRCC	1 cut(s)	741
AccI	GTMKAC	1 cut(s)	2100
AciI	CCGC	3 cut(s)	773, 838, 1066
AcsI	RAATTY	4 cut(s)	1025, 1098, 1576, 2308
AcuI	CTGAAG	1 cut(s)	2391
AfaI	GTAC	7 cut(s)	137, 729, 749, 1018, 1292, 1824, 2073
AfiI	CCNNNNNNNGG	8 cut(s)	203, 279, 340, 779, 858, 1021, 1701, 1754
AflII	CTTAAG	2 cut(s)	1571, 2179
AflIII	ACRYGT	2 cut(s)	1428, 1849
AjnI	CCWGG	1 cut(s)	323
AloI	GAACNNNNNNTCC	2 cut(s)	790, 822
Alw21I	GWGCWC	3 cut(s)	1450, 1728, 2415
Alw26I	GTCTC	9 cut(s)	214, 284, 590, 622, 815, 1249, 1501, 1752, 1851
Alw44I	GTGCAC	1 cut(s)	1446
Ama87I	CYCGRG	1 cut(s)	211
AoxI	GGCC	6 cut(s)	31, 958, 1104, 1460, 1650, 2335
ApaLI	GTGCAC	1 cut(s)	1446
ApoI	RAATTY	4 cut(s)	1025, 1098, 1576, 2308
Asp700I	GAANNNNTTC	1 cut(s)	1257
AspS9I	GGNCC	1 cut(s)	861
AsuC2I	CCSGG	2 cut(s)	1408, 2377
AsuHPI	GGTGA	8 cut(s)	61, 859, 905, 1670, 1976, 2131, 2131, 2441
AsuNHI	GCTAGC	1 cut(s)	757
AvaI	CYCGRG	1 cut(s)	211
AvaII	GGWCC	1 cut(s)	861
BaeGI	GKGCMC	1 cut(s)	1450
BaeI	ACNNNNGTAYC	2 cut(s)	268, 301
BamHI	GGATCC	2 cut(s)	834, 1744
BanI	GGYRCC	1 cut(s)	741
BanII	GRGCYC	2 cut(s)	1728, 2415
BarI	GAAGNNNNNNTAC	2 cut(s)	1274, 1306
BbsI	GAAGAC	1 cut(s)	1560
Bbv12I	GWGCWC	3 cut(s)	1450, 1728, 2415
BccI	CCATC	4 cut(s)	872, 1381, 2261, 2346
BceAI	ACGGC	2 cut(s)	999, 1890
BcgI	CGANNNNNNTGC	2 cut(s)	217, 251
BciT130I	CCWGG	1 cut(s)	325
BclI	TGATCA	3 cut(s)	82, 97, 124
BcnI	CCSGG	2 cut(s)	1408, 2377
BcoDI	GTCTC	9 cut(s)	214, 284, 590, 622, 815, 1249, 1501, 1752, 1851
BfaI	CTAG	3 cut(s)	758, 1400, 2405
BfrI	CTTAAG	2 cut(s)	1571, 2179
BfuAI	ACCTGC	3 cut(s)	37, 762, 2417
Bme1390I	CCNGG	3 cut(s)	325, 1408, 2377
Bme18I	GGWCC	1 cut(s)	861
BmeT110I	CYCGRG	1 cut(s)	211
BmgT120I	GGNCC	1 cut(s)	861
BmiI	GGNNCC	3 cut(s)	743, 836, 1746
BmrFI	CCNGG	3 cut(s)	325, 1408, 2377
BmsI	GCATC	7 cut(s)	80, 432, 551, 1058, 1197, 1597, 1802
BmtI	GCTAGC	1 cut(s)	761
BpiI	GAAGAC	1 cut(s)	1560
BpmI	CTGGAG	2 cut(s)	256, 1380
Bpu10I	CCTNAGC	1 cut(s)	1601
BpuEI	CTTGAG	7 cut(s)	190, 677, 1138, 1231, 1525, 1715, 1814
BpuMI	CCSGG	2 cut(s)	1408, 2377
Bsa29I	ATCGAT	1 cut(s)	426
BsaBI	GATNNNNATC	3 cut(s)	81, 102, 929
BsaI	GGTCTC	1 cut(s)	590
BsaJI	CCNNGG	9 cut(s)	264, 324, 778, 915, 955, 1020, 1082, 1653, 2082
BsaWI	WCCGGW	1 cut(s)	2214
BsaXI	ACNNNNNCTCC	2 cut(s)	2121, 2151
Bsc4I	CCNNNNNNNGG	8 cut(s)	203, 279, 340, 779, 858, 1021, 1701, 1754
Bse118I	RCCGGY	1 cut(s)	1961
Bse1I	ACTGG	1 cut(s)	863
Bse3DI	GCAATG	1 cut(s)	1542
Bse8I	GATNNNNATC	3 cut(s)	81, 102, 929
BseBI	CCWGG	1 cut(s)	325
BseCI	ATCGAT	1 cut(s)	426
BseDI	CCNNGG	9 cut(s)	264, 324, 778, 915, 955, 1020, 1082, 1653, 2082
BseGI	GGATG	2 cut(s)	2338, 2476
BseJI	GATNNNNATC	3 cut(s)	81, 102, 929
BseLI	CCNNNNNNNGG	8 cut(s)	203, 279, 340, 779, 858, 1021, 1701, 1754
BseMI	GCAATG	1 cut(s)	1542
BseMII	CTCAG	5 cut(s)	333, 1122, 1601, 1615, 1623
BseNI	ACTGG	1 cut(s)	863
BseRI	GAGGAG	4 cut(s)	1718, 1817, 2365, 2423
BseSI	GKGCMC	1 cut(s)	1450
BseYI	CCCAGC	1 cut(s)	737
BsgI	GTGCAG	2 cut(s)	1506, 1695
BshFI	GGCC	6 cut(s)	33, 960, 1106, 1462, 1652, 2337
BshNI	GGYRCC	1 cut(s)	741
BshVI	ATCGAT	1 cut(s)	426
BsiHKAI	GWGCWC	3 cut(s)	1450, 1728, 2415
BsiHKCI	CYCGRG	1 cut(s)	211
BsiSI	CCGG	4 cut(s)	1407, 1962, 2215, 2376
BslFI	GGGAC	2 cut(s)	1423, 1439
BslI	CCNNNNNNNGG	8 cut(s)	203, 279, 340, 779, 858, 1021, 1701, 1754
BsmAI	GTCTC	9 cut(s)	214, 284, 590, 622, 815, 1249, 1501, 1752, 1851
BsmBI	CGTCTC	2 cut(s)	622, 815
BsmFI	GGGAC	2 cut(s)	1423, 1439
BsmI	GAATGC	2 cut(s)	1673, 1711
BsnI	GGCC	6 cut(s)	33, 960, 1106, 1462, 1652, 2337
Bso31I	GGTCTC	1 cut(s)	590
BsoBI	CYCGRG	1 cut(s)	211
Bsp1286I	GDGCHC	3 cut(s)	1450, 1728, 2415
Bsp19I	CCATGG	5 cut(s)	264, 915, 1020, 1653, 2082
BspACI	CCGC	3 cut(s)	773, 838, 1066
BspANI	GGCC	6 cut(s)	33, 960, 1106, 1462, 1652, 2337
BspCNI	CTCAG	5 cut(s)	332, 1123, 1602, 1614, 1624
BspDI	ATCGAT	1 cut(s)	426
BspHI	TCATGA	2 cut(s)	64, 94
BspLI	GGNNCC	3 cut(s)	743, 836, 1746
BspMI	ACCTGC	3 cut(s)	37, 762, 2417
BspOI	GCTAGC	1 cut(s)	761
BspQI	GCTCTTC	1 cut(s)	1716
BspT107I	GGYRCC	1 cut(s)	741
BspTI	CTTAAG	2 cut(s)	1571, 2179
BspTNI	GGTCTC	1 cut(s)	590
BsrDI	GCAATG	1 cut(s)	1542
BsrFI	RCCGGY	1 cut(s)	1961
BsrI	ACTGG	1 cut(s)	863
BssAI	RCCGGY	1 cut(s)	1961
BssECI	CCNNGG	9 cut(s)	264, 324, 778, 915, 955, 1020, 1082, 1653, 2082
BssT1I	CCWWGG	7 cut(s)	264, 778, 915, 1020, 1082, 1653, 2082
Bst2UI	CCWGG	1 cut(s)	325
Bst4CI	ACNGT	9 cut(s)	720, 727, 802, 883, 1216, 2005, 2019, 2224, 2495
Bst6I	CTCTTC	2 cut(s)	650, 1716
BstAFI	CTTAAG	2 cut(s)	1571, 2179
BstAPI	GCANNNNNTGC	1 cut(s)	2032
BstC8I	GCNNGC	3 cut(s)	380, 759, 1696
BstDEI	CTNAG	7 cut(s)	319, 391, 994, 1131, 1601, 1610, 1632
BstDSI	CCRYGG	5 cut(s)	264, 915, 1020, 1653, 2082
BstEII	GGTNACC	1 cut(s)	893
BstF5I	GGATG	2 cut(s)	2338, 2476
BstMAI	GTCTC	9 cut(s)	214, 284, 590, 622, 815, 1249, 1501, 1752, 1851
BstMWI	GCNNNNNNNGC	3 cut(s)	320, 1895, 2032
BstNI	CCWGG	1 cut(s)	325
BstNSI	RCATGY	5 cut(s)	946, 1432, 1787, 1853, 2039
BstPI	GGTNACC	1 cut(s)	893
BstSCI	CCNGG	3 cut(s)	323, 1406, 2375
BstSLI	GKGCMC	1 cut(s)	1450
BstV2I	GAAGAC	1 cut(s)	1560
BstX2I	RGATCY	5 cut(s)	160, 834, 1606, 1744, 1920
BstYI	RGATCY	5 cut(s)	160, 834, 1606, 1744, 1920
Bsu15I	ATCGAT	1 cut(s)	426
BsuRI	GGCC	6 cut(s)	33, 960, 1106, 1462, 1652, 2337
BsuTUI	ATCGAT	1 cut(s)	426
BtgI	CCRYGG	5 cut(s)	264, 915, 1020, 1653, 2082
BtsCI	GGATG	2 cut(s)	2338, 2476
BtsI	GCAGTG	3 cut(s)	912, 1518, 1956
BtsIMutI	CAGTG	6 cut(s)	716, 912, 1518, 1956, 2229, 2367
BveI	ACCTGC	3 cut(s)	37, 762, 2417
Cac8I	GCNNGC	3 cut(s)	380, 759, 1696
CciI	TCATGA	2 cut(s)	64, 94
Cfr10I	RCCGGY	1 cut(s)	1961
Cfr13I	GGNCC	1 cut(s)	861
ClaI	ATCGAT	1 cut(s)	426
Csp6I	GTAC	7 cut(s)	136, 728, 748, 1017, 1291, 1823, 2072
CviQI	GTAC	7 cut(s)	136, 728, 748, 1017, 1291, 1823, 2072
DdeI	CTNAG	7 cut(s)	319, 391, 994, 1131, 1601, 1610, 1632
Eam1104I	CTCTTC	2 cut(s)	650, 1716
EarI	CTCTTC	2 cut(s)	650, 1716
Ecl136II	GAGCTC	2 cut(s)	1726, 2413
Eco130I	CCWWGG	7 cut(s)	264, 778, 915, 1020, 1082, 1653, 2082
Eco147I	AGGCCT	1 cut(s)	1462
Eco24I	GRGCYC	2 cut(s)	1728, 2415
Eco31I	GGTCTC	1 cut(s)	590
Eco32I	GATATC	2 cut(s)	430, 512
Eco47I	GGWCC	1 cut(s)	861
Eco53kI	GAGCTC	2 cut(s)	1726, 2413
Eco57I	CTGAAG	1 cut(s)	2391
Eco88I	CYCGRG	1 cut(s)	211
Eco91I	GGTNACC	1 cut(s)	893
EcoICRI	GAGCTC	2 cut(s)	1726, 2413
EcoO65I	GGTNACC	1 cut(s)	893
EcoRII	CCWGG	1 cut(s)	323
EcoRV	GATATC	2 cut(s)	430, 512
EcoT14I	CCWWGG	7 cut(s)	264, 778, 915, 1020, 1082, 1653, 2082
EcoT38I	GRGCYC	2 cut(s)	1728, 2415
ErhI	CCWWGG	7 cut(s)	264, 778, 915, 1020, 1082, 1653, 2082
Esp3I	CGTCTC	2 cut(s)	622, 815
FalI	AAGNNNNNCTT	4 cut(s)	629, 661, 1215, 1247
FaqI	GGGAC	2 cut(s)	1423, 1439
FauI	CCCGC	1 cut(s)	780
FbaI	TGATCA	3 cut(s)	82, 97, 124
FblI	GTMKAC	1 cut(s)	2100
FokI	GGATG	2 cut(s)	2325, 2483
FriOI	GRGCYC	2 cut(s)	1728, 2415
FspBI	CTAG	3 cut(s)	758, 1400, 2405
GsaI	CCCAGC	1 cut(s)	741
GsuI	CTGGAG	2 cut(s)	256, 1380
HaeIII	GGCC	6 cut(s)	33, 960, 1106, 1462, 1652, 2337
HapII	CCGG	4 cut(s)	1407, 1962, 2215, 2376
HincII	GTYRAC	3 cut(s)	1063, 1196, 2041
HindII	GTYRAC	3 cut(s)	1063, 1196, 2041
HindIII	AAGCTT	3 cut(s)	185, 1468, 2522
HinfI	GANTC	8 cut(s)	229, 269, 488, 826, 1093, 1509, 1760, 2042
HpaII	CCGG	4 cut(s)	1407, 1962, 2215, 2376
HphI	GGTGA	8 cut(s)	61, 859, 905, 1670, 1976, 2131, 2131, 2441
Hpy188I	TCNGA	9 cut(s)	76, 160, 614, 692, 818, 1482, 1611, 2001, 2261
Hpy99I	CGWCG	6 cut(s)	157, 160, 364, 367, 452, 2060
HpyAV	CCTTC	6 cut(s)	655, 998, 1276, 2005, 2178, 2471
HpyCH4III	ACNGT	9 cut(s)	720, 727, 802, 883, 1216, 2005, 2019, 2224, 2495
HpyF10VI	GCNNNNNNNGC	3 cut(s)	320, 1895, 2032
HpyF3I	CTNAG	7 cut(s)	319, 391, 994, 1131, 1601, 1610, 1632
Ksp22I	TGATCA	3 cut(s)	82, 97, 124
LguI	GCTCTTC	1 cut(s)	1716
LmnI	GCTCC	6 cut(s)	615, 1170, 1731, 2129, 2410, 2480
LweI	GCATC	7 cut(s)	80, 432, 551, 1058, 1197, 1597, 1802
MaeI	CTAG	3 cut(s)	758, 1400, 2405
MaeIII	GTNAC	6 cut(s)	712, 893, 1841, 1868, 2137, 2344
MfeI	CAATTG	1 cut(s)	1890
MflI	RGATCY	5 cut(s)	160, 834, 1606, 1744, 1920
MhlI	GDGCHC	3 cut(s)	1450, 1728, 2415
MlyI	GAGTC	7 cut(s)	223, 263, 482, 835, 1503, 1754, 2036
MmeI	TCCRAC	3 cut(s)	138, 1722, 2188
MroXI	GAANNNNTTC	1 cut(s)	1257
MseI	TTAA	6 cut(s)	54, 1125, 1572, 2180, 2465, 2554
MslI	CAYNNNNRTG	2 cut(s)	872, 920
MspA1I	CMGCKG	1 cut(s)	1982
MspCI	CTTAAG	2 cut(s)	1571, 2179
MspI	CCGG	4 cut(s)	1407, 1962, 2215, 2376
MspR9I	CCNGG	3 cut(s)	325, 1408, 2377
MunI	CAATTG	1 cut(s)	1890
Mva1269I	GAATGC	2 cut(s)	1673, 1711
MvaI	CCWGG	1 cut(s)	325
MwoI	GCNNNNNNNGC	3 cut(s)	320, 1895, 2032
NciI	CCSGG	2 cut(s)	1408, 2377
NcoI	CCATGG	5 cut(s)	264, 915, 1020, 1653, 2082
NheI	GCTAGC	1 cut(s)	757
NlaIV	GGNNCC	3 cut(s)	743, 836, 1746
NmeAIII	GCCGAG	1 cut(s)	936
NmuCI	GTSAC	4 cut(s)	712, 893, 2137, 2344
NspI	RCATGY	5 cut(s)	946, 1432, 1787, 1853, 2039
PaeR7I	CTCGAG	1 cut(s)	211
PagI	TCATGA	2 cut(s)	64, 94
PaqCI	CACCTGC	2 cut(s)	37, 2417
PceI	AGGCCT	1 cut(s)	1462
PciI	ACATGT	2 cut(s)	1428, 1849
PciSI	GCTCTTC	1 cut(s)	1716
PcsI	WCGNNNNNNNCGW	1 cut(s)	1332
PctI	GAATGC	2 cut(s)	1673, 1711
PdmI	GAANNNNTTC	1 cut(s)	1257
PfeI	GAWTC	1 cut(s)	1093
PleI	GAGTC	7 cut(s)	223, 263, 482, 834, 1503, 1754, 2036
PpsI	GAGTC	7 cut(s)	223, 263, 482, 834, 1503, 1754, 2036
PscI	ACATGT	2 cut(s)	1428, 1849
Psp124BI	GAGCTC	2 cut(s)	1728, 2415
Psp6I	CCWGG	1 cut(s)	323
PspEI	GGTNACC	1 cut(s)	893
PspFI	CCCAGC	1 cut(s)	737
PspGI	CCWGG	1 cut(s)	323
PspN4I	GGNNCC	3 cut(s)	743, 836, 1746
PspPI	GGNCC	1 cut(s)	861
PsuI	RGATCY	5 cut(s)	160, 834, 1606, 1744, 1920
PvuII	CAGCTG	1 cut(s)	1982
RsaI	GTAC	7 cut(s)	137, 729, 749, 1018, 1292, 1824, 2073
RsaNI	GTAC	7 cut(s)	136, 728, 748, 1017, 1291, 1823, 2072
RseI	CAYNNNNRTG	2 cut(s)	872, 920
SacI	GAGCTC	2 cut(s)	1728, 2415
SapI	GCTCTTC	1 cut(s)	1716
SaqAI	TTAA	6 cut(s)	54, 1125, 1572, 2180, 2465, 2554
Sau96I	GGNCC	1 cut(s)	861
SchI	GAGTC	7 cut(s)	223, 263, 482, 835, 1503, 1754, 2036
ScrFI	CCNGG	3 cut(s)	325, 1408, 2377
SduI	GDGCHC	3 cut(s)	1450, 1728, 2415
SfaNI	GCATC	7 cut(s)	80, 432, 551, 1058, 1197, 1597, 1802
Sfr274I	CTCGAG	1 cut(s)	211
SinI	GGWCC	1 cut(s)	861
SlaI	CTCGAG	1 cut(s)	211
SmiMI	CAYNNNNRTG	2 cut(s)	872, 920
SseBI	AGGCCT	1 cut(s)	1462
SsiI	CCGC	3 cut(s)	773, 838, 1066
SspI	AATATT	3 cut(s)	1626, 2422, 2463
SspMI	CTAG	3 cut(s)	758, 1400, 2405
SstI	GAGCTC	2 cut(s)	1728, 2415
StuI	AGGCCT	1 cut(s)	1462
StyD4I	CCNGG	3 cut(s)	323, 1406, 2375
StyI	CCWWGG	7 cut(s)	264, 778, 915, 1020, 1082, 1653, 2082
TaaI	ACNGT	9 cut(s)	720, 727, 802, 883, 1216, 2005, 2019, 2224, 2495
TaqI	TCGA	7 cut(s)	212, 426, 486, 518, 1677, 2055, 2499
TaqII	GACCGA	2 cut(s)	572, 780
TatI	WGTACW	5 cut(s)	135, 727, 747, 1290, 2071
TfiI	GAWTC	1 cut(s)	1093
Tru1I	TTAA	6 cut(s)	54, 1125, 1572, 2180, 2465, 2554
Tru9I	TTAA	6 cut(s)	54, 1125, 1572, 2180, 2465, 2554
TscAI	CASTG	6 cut(s)	723, 912, 1525, 1963, 2229, 2374
TseFI	GTSAC	4 cut(s)	712, 893, 2137, 2344
Tsp45I	GTSAC	4 cut(s)	712, 893, 2137, 2344
TspGWI	ACGGA	2 cut(s)	2073, 2483
TspRI	CASTG	6 cut(s)	723, 912, 1525, 1963, 2229, 2374
Vha464I	CTTAAG	2 cut(s)	1571, 2179
VneI	GTGCAC	1 cut(s)	1446
VpaK11BI	GGWCC	1 cut(s)	861
XapI	RAATTY	4 cut(s)	1025, 1098, 1576, 2308
XceI	RCATGY	5 cut(s)	946, 1432, 1787, 1853, 2039
XhoI	CTCGAG	1 cut(s)	211
XmiI	GTMKAC	1 cut(s)	2100
XmnI	GAANNNNTTC	1 cut(s)	1257
XspI	CTAG	3 cut(s)	758, 1400, 2405

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.