FvH4_2g04220

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0002575
Physical Location & Seq
Forward (+)
3300680 .. 3302762
2083 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g04220.t1

Sequence Viewer

Length: 1014 bp
ATGGAGTACGTTGGAGCTGTTTCAGAAGGAGAATGGAGCTCATTGAGTGGGATGTACACTGCTGAGGAAGCGGATTTCATGGCCAGGTTGCTTGAGAACTGTCCACTTCCAAGCATGCCAGGCATGACATTCCCAGATGCTTTTTGGCATGGCAATGAAGAATCAACAATGCACATGTCAGGCAACTATGAAGGTTCACATTGTTCTTTAGAAACAACTAATTCCAATTTCATCACTTCAGACAACGAAAACTACTACTTGAGTGATTCTCACAGAATGTTGGCAACAAACAACAATAGCAATAGCTCTATGTCGATTGATTTTGGTATCGGGGATGTGAAAACCATCAATACCTATCTCAGTGAAGGAAATGATCATGACCAATGCTCAAACCGAGAAACAAGTAAAGGCAACATCGAAGAGTCTGGTGGAAGCAAGCATGAAGCTGTCAATCTTGAGAAGAGGAGATCTCAAAGTAGCCTCCCCAAGATTAACTCCATGAAAAGATCTCAAAGTACAGACAATGTTCAAATGAACAAGAGGAGCAAGGCAAAGAAGAGCCTGCGGGTTACAACTAGCAACATCGAAGATGGCTATGCTGGCCCCAATAGACAGACTTCGAGTAGTTGCTGCTCAGAAGATGACTCCATTGTTTATGAGGTGAGTGGAGGGTTTAGTCCAACTTCAAGCTTGAGTCCAAAGGAGGATGCAGTTCCTGACTTGAATGGCAAATCGAGAGCACGAAGGGGGTCAGCCAGTGATCCGCAGAGTCTCTATGCAAGGAAAAGAAGAGAGAAAATAAATGAGAGGCTCAGAGTTCTGCAGAATCTTGTCCCCAATGGAACAAAGGTTGATATTAGCACCATGCTTGAGGAAGCGGTCCATTATGTGAAGTTCTTACAACTCCAAATTAAGTTGTTAAGCTCTGATGACATGTGGATGTATGCTCCCATCTGTTACAATGGAATGGACCTTGGACTTGATCGGAAACTCACCACACCAAGCCAACCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000902 GO:0000904 GO:0000976 GO:0000981 GO:0001046 GO:0001047 GO:0001067 GO:0001228 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009888 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010015 GO:0010033 GO:0010053 GO:0010054 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016043 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0021700 GO:0022622 GO:0030154 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032501 GO:0032502 GO:0032774 GO:0032989 GO:0034641 GO:0034645 GO:0034654 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048364 GO:0048468 GO:0048469 GO:0048518 GO:0048522 GO:0048731 GO:0048764 GO:0048765 GO:0048766 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0060255 GO:0065007 GO:0071695 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0090558 GO:0090627 GO:0097159 GO:0097659 GO:0099402 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:1905392 GO:1990837 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HLH

Protein Analysis

338

Amino Acids

37.45

Weight (kDa)

5.39

Isoelectric Point (pI)

64.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 261 - 301 2.3e-08 Helix-loop-helix DNA-binding domain
bHLH_SAC51 PF23173 262 - 308 7.8e-07 Transcription factor SAC51 bHLH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 71, 565, 764, 878
AclWI GGATC 1 cut(s) 755
AcoI YGGCCR 1 cut(s) 81
AcuI CTGAAG 1 cut(s) 222
AfaI GTAC 3 cut(s) 8, 56, 517
AflIII ACRYGT 2 cut(s) 174, 933
AgsI TTSAA 3 cut(s) 530, 687, 724
AjnI CCWGG 2 cut(s) 83, 118
AluBI AGCT 6 cut(s) 17, 39, 306, 446, 690, 924
AluI AGCT 6 cut(s) 17, 39, 306, 446, 690, 924
Alw21I GWGCWC 2 cut(s) 41, 742
Alw26I GTCTC 1 cut(s) 776
AlwI GGATC 1 cut(s) 755
AlwNI CAGNNNCTG 1 cut(s) 716
AoxI GGCC 2 cut(s) 81, 601
ApeKI GCWGC 1 cut(s) 630
AspS9I GGNCC 3 cut(s) 602, 880, 970
AsuHPI GGTGA 2 cut(s) 673, 985
AvaII GGWCC 2 cut(s) 880, 970
BalI TGGCCA 1 cut(s) 83
BanII GRGCYC 1 cut(s) 41
Bbv12I GWGCWC 2 cut(s) 41, 742
BbvCI CCTCAGC 1 cut(s) 63
BbvI GCAGC 1 cut(s) 617
BccI CCATC 3 cut(s) 353, 584, 959
BciT130I CCWGG 2 cut(s) 85, 120
BclI TGATCA 1 cut(s) 373
BcoDI GTCTC 1 cut(s) 776
BfaI CTAG 1 cut(s) 576
BfmI CTRYAG 1 cut(s) 821
BglII AGATCT 2 cut(s) 467, 506
BisI GCNGC 1 cut(s) 631
BlsI GCNGC 1 cut(s) 632
Bme1390I CCNGG 2 cut(s) 85, 120
Bme18I GGWCC 2 cut(s) 880, 970
BmgT120I GGNCC 3 cut(s) 602, 880, 970
BmiI GGNNCC 1 cut(s) 604
BmrFI CCNGG 2 cut(s) 85, 120
BmsI GCATC 2 cut(s) 127, 697
BplI GAGNNNNNCTC 4 cut(s) 253, 285, 454, 486
Bpu10I CCTNAGC 1 cut(s) 63
BpuEI CTTGAG 5 cut(s) 113, 280, 476, 712, 890
BsaJI CCNNGG 1 cut(s) 973
Bse1I ACTGG 1 cut(s) 756
Bse3DI GCAATG 1 cut(s) 160
BseBI CCWGG 2 cut(s) 85, 120
BseDI CCNNGG 1 cut(s) 973
BseGI GGATG 4 cut(s) 57, 340, 712, 945
BseMI GCAATG 1 cut(s) 160
BseMII CTCAG 4 cut(s) 54, 373, 648, 826
BseNI ACTGG 1 cut(s) 756
BseRI GAGGAG 2 cut(s) 478, 556
BseXI GCAGC 1 cut(s) 617
BshFI GGCC 2 cut(s) 83, 603
BsiHKAI GWGCWC 2 cut(s) 41, 742
BslFI GGGAC 1 cut(s) 818
BsmAI GTCTC 1 cut(s) 776
BsmFI GGGAC 1 cut(s) 818
BsnI GGCC 2 cut(s) 83, 603
Bsp1286I GDGCHC 2 cut(s) 41, 742
Bsp1407I TGTACA 1 cut(s) 54
Bsp143I GATC 5 cut(s) 373, 467, 506, 760, 982
BspACI CCGC 4 cut(s) 71, 565, 764, 878
BspANI GGCC 2 cut(s) 83, 603
BspCNI CTCAG 4 cut(s) 55, 372, 647, 825
BspHI TCATGA 1 cut(s) 376
BspLI GGNNCC 1 cut(s) 604
BspMAI CTGCAG 1 cut(s) 825
BspPI GGATC 1 cut(s) 755
BspQI GCTCTTC 1 cut(s) 551
BsrDI GCAATG 1 cut(s) 160
BsrGI TGTACA 1 cut(s) 54
BsrI ACTGG 1 cut(s) 756
BssECI CCNNGG 1 cut(s) 973
BssMI GATC 5 cut(s) 373, 467, 506, 760, 982
BssT1I CCWWGG 1 cut(s) 973
Bst2UI CCWGG 2 cut(s) 85, 120
Bst4CI ACNGT 2 cut(s) 101, 1011
Bst6I CTCTTC 4 cut(s) 414, 455, 551, 784
BstAUI TGTACA 1 cut(s) 54
BstC8I GCNNGC 4 cut(s) 116, 437, 563, 601
BstDEI CTNAG 4 cut(s) 63, 359, 634, 812
BstF5I GGATG 4 cut(s) 57, 340, 712, 945
BstKTI GATC 5 cut(s) 376, 470, 509, 763, 985
BstMAI GTCTC 1 cut(s) 776
BstMBI GATC 5 cut(s) 373, 467, 506, 760, 982
BstMWI GCNNNNNNNGC 3 cut(s) 68, 120, 600
BstNI CCWGG 2 cut(s) 85, 120
BstNSI RCATGY 3 cut(s) 118, 178, 937
BstSCI CCNGG 2 cut(s) 83, 118
BstSFI CTRYAG 1 cut(s) 821
BstV1I GCAGC 1 cut(s) 617
BstX2I RGATCY 2 cut(s) 467, 506
BstYI RGATCY 2 cut(s) 467, 506
BsuRI GGCC 2 cut(s) 83, 603
BtsCI GGATG 4 cut(s) 57, 340, 712, 945
BtsI GCAGTG 1 cut(s) 57
BtsIMutI CAGTG 3 cut(s) 57, 367, 763
Cac8I GCNNGC 4 cut(s) 116, 437, 563, 601
CaiI CAGNNNCTG 1 cut(s) 716
CciI TCATGA 1 cut(s) 376
Cfr13I GGNCC 3 cut(s) 602, 880, 970
Csp6I GTAC 3 cut(s) 7, 55, 516
CviQI GTAC 3 cut(s) 7, 55, 516
DdeI CTNAG 4 cut(s) 63, 359, 634, 812
DpnI GATC 5 cut(s) 375, 469, 508, 762, 984
DpnII GATC 5 cut(s) 373, 467, 506, 760, 982
EaeI YGGCCR 1 cut(s) 81
Eam1104I CTCTTC 4 cut(s) 414, 455, 551, 784
EarI CTCTTC 4 cut(s) 414, 455, 551, 784
Ecl136II GAGCTC 1 cut(s) 39
Eco130I CCWWGG 1 cut(s) 973
Eco24I GRGCYC 1 cut(s) 41
Eco47I GGWCC 2 cut(s) 880, 970
Eco53kI GAGCTC 1 cut(s) 39
Eco57I CTGAAG 1 cut(s) 222
EcoICRI GAGCTC 1 cut(s) 39
EcoRII CCWGG 2 cut(s) 83, 118
EcoT14I CCWWGG 1 cut(s) 973
EcoT38I GRGCYC 1 cut(s) 41
ErhI CCWWGG 1 cut(s) 973
FaqI GGGAC 1 cut(s) 818
FauI CCCGC 1 cut(s) 558
FbaI TGATCA 1 cut(s) 373
Fnu4HI GCNGC 1 cut(s) 631
FokI GGATG 4 cut(s) 64, 347, 719, 952
FriOI GRGCYC 1 cut(s) 41
Fsp4HI GCNGC 1 cut(s) 631
FspBI CTAG 1 cut(s) 576
GluI GCNGC 1 cut(s) 631
HaeIII GGCC 2 cut(s) 83, 603
HindIII AAGCTT 1 cut(s) 688
HinfI GANTC 7 cut(s) 161, 266, 422, 644, 694, 769, 826
HphI GGTGA 2 cut(s) 673, 985
Hpy166II GTNNAC 3 cut(s) 57, 104, 197
Hpy188I TCNGA 6 cut(s) 25, 241, 637, 815, 928, 987
Hpy188III TCNNGA 4 cut(s) 377, 455, 716, 735
Hpy8I GTNNAC 3 cut(s) 57, 104, 197
HpyAV CCTTC 4 cut(s) 20, 185, 359, 738
HpyCH4III ACNGT 2 cut(s) 101, 1011
HpyCH4IV ACGT 1 cut(s) 9
HpyCH4V TGCA 4 cut(s) 172, 710, 779, 823
HpyF10VI GCNNNNNNNGC 3 cut(s) 68, 120, 600
HpyF3I CTNAG 4 cut(s) 63, 359, 634, 812
HpySE526I ACGT 1 cut(s) 9
Ksp22I TGATCA 1 cut(s) 373
Kzo9I GATC 5 cut(s) 373, 467, 506, 760, 982
LguI GCTCTTC 1 cut(s) 551
LmnI GCTCC 4 cut(s) 14, 36, 543, 952
Lsp1109I GCAGC 1 cut(s) 617
LweI GCATC 2 cut(s) 127, 697
MaeI CTAG 1 cut(s) 576
MaeII ACGT 1 cut(s) 9
MaeIII GTNAC 2 cut(s) 568, 956
MalI GATC 5 cut(s) 375, 469, 508, 762, 984
MboI GATC 5 cut(s) 373, 467, 506, 760, 982
MboII GAAGA 7 cut(s) 170, 431, 472, 568, 599, 650, 801
MflI RGATCY 2 cut(s) 467, 506
MhlI GDGCHC 2 cut(s) 41, 742
MlsI TGGCCA 1 cut(s) 83
MluCI AATT 3 cut(s) 220, 226, 909
MluNI TGGCCA 1 cut(s) 83
MlyI GAGTC 4 cut(s) 431, 638, 703, 778
MmeI TCCRAC 1 cut(s) 704
MnlI CCTC 9 cut(s) 58, 456, 491, 534, 652, 662, 697, 801, 865
Mox20I TGGCCA 1 cut(s) 83
MscI TGGCCA 1 cut(s) 83
MseI TTAA 3 cut(s) 492, 912, 920
MslI CAYNNNNRTG 2 cut(s) 153, 938
Msp20I TGGCCA 1 cut(s) 83
MspR9I CCNGG 2 cut(s) 85, 120
MvaI CCWGG 2 cut(s) 85, 120
MwoI GCNNNNNNNGC 3 cut(s) 68, 120, 600
NdeII GATC 5 cut(s) 373, 467, 506, 760, 982
NlaIV GGNNCC 1 cut(s) 604
NspI RCATGY 3 cut(s) 118, 178, 937
PaeI GCATGC 1 cut(s) 118
PagI TCATGA 1 cut(s) 376
PciI ACATGT 2 cut(s) 174, 933
PciSI GCTCTTC 1 cut(s) 551
PfeI GAWTC 3 cut(s) 161, 266, 826
PkrI GCNGC 1 cut(s) 632
PleI GAGTC 4 cut(s) 430, 638, 702, 777
PpsI GAGTC 4 cut(s) 430, 638, 702, 777
PscI ACATGT 2 cut(s) 174, 933
Psp124BI GAGCTC 1 cut(s) 41
Psp6I CCWGG 2 cut(s) 83, 118
PspGI CCWGG 2 cut(s) 83, 118
PspN4I GGNNCC 1 cut(s) 604
PspPI GGNCC 3 cut(s) 602, 880, 970
PstI CTGCAG 1 cut(s) 825
PstNI CAGNNNCTG 1 cut(s) 716
PsuI RGATCY 2 cut(s) 467, 506
RsaI GTAC 3 cut(s) 8, 56, 517
RsaNI GTAC 3 cut(s) 7, 55, 516
RseI CAYNNNNRTG 2 cut(s) 153, 938
SacI GAGCTC 1 cut(s) 41
SapI GCTCTTC 1 cut(s) 551
SaqAI TTAA 3 cut(s) 492, 912, 920
SatI GCNGC 1 cut(s) 631
Sau3AI GATC 5 cut(s) 373, 467, 506, 760, 982
Sau96I GGNCC 3 cut(s) 602, 880, 970
SchI GAGTC 4 cut(s) 431, 638, 703, 778
ScrFI CCNGG 2 cut(s) 85, 120
SduI GDGCHC 2 cut(s) 41, 742
SfaNI GCATC 2 cut(s) 127, 697
SfcI CTRYAG 1 cut(s) 821
SinI GGWCC 2 cut(s) 880, 970
SmiMI CAYNNNNRTG 2 cut(s) 153, 938
SmlI CTYRAG 5 cut(s) 92, 259, 455, 691, 869
SmoI CTYRAG 5 cut(s) 92, 259, 455, 691, 869
SphI GCATGC 1 cut(s) 118
Sse9I AATT 3 cut(s) 220, 226, 909
SsiI CCGC 4 cut(s) 71, 565, 764, 878
SspMI CTAG 1 cut(s) 576
SstI GAGCTC 1 cut(s) 41
StyD4I CCNGG 2 cut(s) 83, 118
StyI CCWWGG 1 cut(s) 973
TaaI ACNGT 2 cut(s) 101, 1011
TaiI ACGT 1 cut(s) 12
TaqI TCGA 5 cut(s) 314, 417, 585, 620, 734
TasI AATT 3 cut(s) 220, 226, 909
TatI WGTACW 2 cut(s) 54, 515
TfiI GAWTC 3 cut(s) 161, 266, 826
Tru1I TTAA 3 cut(s) 492, 912, 920
Tru9I TTAA 3 cut(s) 492, 912, 920
TscAI CASTG 3 cut(s) 64, 367, 763
TseI GCWGC 1 cut(s) 630
TspDTI ATGAA 7 cut(s) 67, 171, 204, 220, 456, 515, 548
TspRI CASTG 3 cut(s) 64, 367, 763
VpaK11BI GGWCC 2 cut(s) 880, 970
XceI RCATGY 3 cut(s) 118, 178, 937
XcmI CCANNNNNNNNNTGG 1 cut(s) 141
XspI CTAG 1 cut(s) 576
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.