MD10G1104900.v1.1

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0002575
Physical Location & Seq
Forward (+)
17123720 .. 17125773
2054 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1104900.v1.1.491

Sequence Viewer

Length: 1098 bp
ATGGAGCATAGTGAAGCTGTTTCAGAGGGAGAATGGACCTCTCTCAGTGGGATGTACATTGCTGAGGAGGTAGATTTCATGTCCCAGTTGCTTGGAAAATTTTCAAACCAAACATCAAGCATGGGAATAGCTCCGGATGCTTTTTGGTCTGGTGGCAACTATGAAGGTTCAAATTATTATTCATCAGAAACATCTTATTCAAATTCACTGGAGGGTAATTTTAGTGGTGGTAATAGGACTTTTTACCCCACTTCAAGCAGTGAGAGTTACTACTTGAGTGAATCTTCTCACCCCATGTTGGTAACAAACAACAGCAAAATCTCTATGTCTATTGATTTTGGTGCTGGAGATGTGATCAAAACCCTAAATTCCTCTCTCATTGAAGGAGATCATGATCAATGCTTGAACCAAGAAACAAGCGATGGCAATGCAGAAGAGAGTGGTGGAAACCCGGCGGAAGCTGTGGCCCTTGGCAATCAGAGATTTGATATGACTGCAGTAAAAGAACAATCCATGGAGGAAATTAACCCCACCAAGAATTCATTGAAAGTATCTCAAAGCTTAGAAAATGTTCAAATGAATAAGAGGAACATAAAGGCAAAGAAAAGCCAGCAGGTGACAGCTAGCAACAATGAAGAAGACGGTAATGCCGCTGGGCTCAACAGACAGAGCTACAGTTGCTGCTCAGGAGATGATCAGTCCAATATTGTTGCCTCACATTCCCATGAGAATTCTCAGGAGCTGAGTGGAGTTAGTATTACTTCAACTTCAAGCTTGAGTCCAACAGAGGTTGCAGCTCTCAACTTGAGTGGCAAATCAAGAGCTAGAAGGGGGTCAGCCACTGATCCCCAAAGCCTCTATGCAAGGAAAAGAAGAGAGAAAATAAATGCGAGGCTGAGAGTCCTGCAGGGCCTTATCCCCAATGGAACAAAGGTTGATATTAGCACAATGCTTGAGGAAGCTGTCCATTATGTGAAATTTTTACAACTCCAAATCAAGCTGTTAAGCTCTGATGATACGTGGATGTATGCTCCCATCTATTACAGTGGAATGAACTTAGGACTTGATCTGAAGCTCACCACACCACAGCAATCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000902 GO:0000904 GO:0000976 GO:0000981 GO:0001046 GO:0001047 GO:0001067 GO:0001228 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009888 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010015 GO:0010033 GO:0010053 GO:0010054 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016043 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0021700 GO:0022622 GO:0030154 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032501 GO:0032502 GO:0032774 GO:0032989 GO:0034641 GO:0034645 GO:0034654 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048364 GO:0048468 GO:0048469 GO:0048518 GO:0048522 GO:0048731 GO:0048764 GO:0048765 GO:0048766 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0060255 GO:0065007 GO:0071695 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0090558 GO:0090627 GO:0097159 GO:0097659 GO:0099402 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:1905392 GO:1990837 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HLH

Protein Analysis

366

Amino Acids

39.78

Weight (kDa)

4.89

Isoelectric Point (pI)

53.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 289 - 329 2.9e-07 Helix-loop-helix DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 604
Acc36I ACCTGC 1 cut(s) 604
AccIII TCCGGA 1 cut(s) 133
AciI CCGC 2 cut(s) 455, 651
AclWI GGATC 1 cut(s) 839
AcsI RAATTY 6 cut(s) 98, 202, 367, 538, 730, 977
AcuI CTGAAG 1 cut(s) 1091
AfaI GTAC 1 cut(s) 56
AfiI CCNNNNNNNGG 1 cut(s) 298
AlwI GGATC 1 cut(s) 839
AlwNI CAGNNNCTG 3 cut(s) 681, 742, 842
Aor13HI TCCGGA 1 cut(s) 133
AoxI GGCC 2 cut(s) 465, 910
ApeKI GCWGC 2 cut(s) 681, 794
ApoI RAATTY 6 cut(s) 98, 202, 367, 538, 730, 977
Asp700I GAANNNNTTC 2 cut(s) 100, 570
AspS9I GGNCC 3 cut(s) 36, 466, 910
AsuC2I CCSGG 1 cut(s) 452
AsuHPI GGTGA 3 cut(s) 281, 628, 1069
AsuNHI GCTAGC 1 cut(s) 623
AvaII GGWCC 1 cut(s) 36
BanII GRGCYC 1 cut(s) 660
BbsI GAAGAC 1 cut(s) 645
BbvCI CCTCAGC 1 cut(s) 63
BbvI GCAGC 2 cut(s) 668, 806
BccI CCATC 2 cut(s) 416, 1043
BcgI CGANNNNNNTGC 2 cut(s) 410, 444
BclI TGATCA 3 cut(s) 354, 394, 694
BcnI CCSGG 1 cut(s) 452
BfaI CTAG 2 cut(s) 624, 825
BfmI CTRYAG 3 cut(s) 495, 673, 905
BfuAI ACCTGC 1 cut(s) 604
BisI GCNGC 3 cut(s) 651, 682, 795
BlsI GCNGC 3 cut(s) 652, 683, 796
Bme1390I CCNGG 1 cut(s) 452
Bme18I GGWCC 1 cut(s) 36
BmgT120I GGNCC 3 cut(s) 36, 466, 910
BmrFI CCNGG 1 cut(s) 452
BmrI ACTGGG 1 cut(s) 79
BmsI GCATC 1 cut(s) 127
BmtI GCTAGC 1 cut(s) 627
BmuI ACTGGG 1 cut(s) 79
BpiI GAAGAC 1 cut(s) 645
BpmI CTGGAG 2 cut(s) 230, 366
Bpu10I CCTNAGC 2 cut(s) 63, 685
BpuEI CTTGAG 4 cut(s) 295, 796, 826, 974
BpuMI CCSGG 1 cut(s) 452
BsaAI YACGTR 1 cut(s) 1020
BsaBI GATNNNNATC 1 cut(s) 393
BsaJI CCNNGG 2 cut(s) 469, 513
BsaWI WCCGGW 1 cut(s) 133
Bsc4I CCNNNNNNNGG 1 cut(s) 298
Bse1I ACTGG 2 cut(s) 85, 213
Bse3DI GCAATG 2 cut(s) 57, 433
Bse8I GATNNNNATC 1 cut(s) 393
BseAI TCCGGA 1 cut(s) 133
BseDI CCNNGG 2 cut(s) 469, 513
BseGI GGATG 3 cut(s) 57, 142, 1029
BseJI GATNNNNATC 1 cut(s) 393
BseLI CCNNNNNNNGG 1 cut(s) 298
BseMI GCAATG 2 cut(s) 57, 433
BseMII CTCAG 6 cut(s) 54, 58, 699, 734, 749, 887
BseNI ACTGG 2 cut(s) 85, 213
BseRI GAGGAG 1 cut(s) 80
BseXI GCAGC 2 cut(s) 668, 806
BseYI CCCAGC 1 cut(s) 653
BshFI GGCC 2 cut(s) 467, 912
BsiSI CCGG 2 cut(s) 134, 452
BslFI GGGAC 1 cut(s) 67
BslI CCNNNNNNNGG 1 cut(s) 298
BsmFI GGGAC 1 cut(s) 67
BsnI GGCC 2 cut(s) 467, 912
Bsp1286I GDGCHC 1 cut(s) 660
Bsp13I TCCGGA 1 cut(s) 133
Bsp1407I TGTACA 1 cut(s) 54
Bsp143I GATC 6 cut(s) 354, 388, 394, 694, 844, 1066
Bsp19I CCATGG 1 cut(s) 513
BspACI CCGC 2 cut(s) 455, 651
BspANI GGCC 2 cut(s) 467, 912
BspCNI CTCAG 6 cut(s) 55, 57, 698, 735, 748, 888
BspEI TCCGGA 1 cut(s) 133
BspHI TCATGA 1 cut(s) 391
BspMAI CTGCAG 2 cut(s) 499, 909
BspMI ACCTGC 1 cut(s) 604
BspOI GCTAGC 1 cut(s) 627
BspPI GGATC 1 cut(s) 839
BsrDI GCAATG 2 cut(s) 57, 433
BsrGI TGTACA 1 cut(s) 54
BsrI ACTGG 2 cut(s) 85, 213
BssECI CCNNGG 2 cut(s) 469, 513
BssMI GATC 6 cut(s) 354, 388, 394, 694, 844, 1066
BssT1I CCWWGG 2 cut(s) 469, 513
Bst4CI ACNGT 3 cut(s) 644, 677, 1046
Bst6I CTCTTC 2 cut(s) 429, 868
BstAUI TGTACA 1 cut(s) 54
BstBAI YACGTR 1 cut(s) 1020
BstC8I GCNNGC 2 cut(s) 611, 625
BstDEI CTNAG 8 cut(s) 44, 63, 562, 685, 735, 743, 896, 1057
BstDSI CCRYGG 1 cut(s) 513
BstF5I GGATG 3 cut(s) 57, 142, 1029
BstKTI GATC 6 cut(s) 357, 391, 397, 697, 847, 1069
BstMBI GATC 6 cut(s) 354, 388, 394, 694, 844, 1066
BstMWI GCNNNNNNNGC 2 cut(s) 137, 678
BstSCI CCNGG 1 cut(s) 450
BstSFI CTRYAG 3 cut(s) 495, 673, 905
BstV1I GCAGC 2 cut(s) 668, 806
BstV2I GAAGAC 1 cut(s) 645
BstXI CCANNNNNNTGG 1 cut(s) 92
BsuRI GGCC 2 cut(s) 467, 912
BtgI CCRYGG 1 cut(s) 513
BtgZI GCGATG 1 cut(s) 435
BtsCI GGATG 3 cut(s) 57, 142, 1029
BtsI GCAGTG 1 cut(s) 265
BtsIMutI CAGTG 5 cut(s) 52, 206, 265, 840, 1051
BveI ACCTGC 1 cut(s) 604
Cac8I GCNNGC 2 cut(s) 611, 625
CaiI CAGNNNCTG 3 cut(s) 681, 742, 842
CciI TCATGA 1 cut(s) 391
Cfr13I GGNCC 3 cut(s) 36, 466, 910
Csp6I GTAC 1 cut(s) 55
CspCI CAANNNNNGTGG 2 cut(s) 790, 825
CviAII CATG 6 cut(s) 79, 121, 295, 392, 514, 725
CviQI GTAC 1 cut(s) 55
DdeI CTNAG 8 cut(s) 44, 63, 562, 685, 735, 743, 896, 1057
DpnI GATC 6 cut(s) 356, 390, 396, 696, 846, 1068
DpnII GATC 6 cut(s) 354, 388, 394, 694, 844, 1066
Eam1104I CTCTTC 2 cut(s) 429, 868
EarI CTCTTC 2 cut(s) 429, 868
EciI GGCGGA 1 cut(s) 470
Eco130I CCWWGG 2 cut(s) 469, 513
Eco24I GRGCYC 1 cut(s) 660
Eco47I GGWCC 1 cut(s) 36
Eco57I CTGAAG 1 cut(s) 1091
EcoO109I RGGNCCY 1 cut(s) 910
EcoRI GAATTC 2 cut(s) 538, 730
EcoT14I CCWWGG 2 cut(s) 469, 513
EcoT38I GRGCYC 1 cut(s) 660
ErhI CCWWGG 2 cut(s) 469, 513
FaeI CATG 6 cut(s) 82, 124, 298, 395, 517, 728
FaqI GGGAC 1 cut(s) 67
FatI CATG 6 cut(s) 78, 120, 294, 391, 513, 724
FbaI TGATCA 3 cut(s) 354, 394, 694
Fnu4HI GCNGC 3 cut(s) 651, 682, 795
FokI GGATG 3 cut(s) 64, 149, 1036
FriOI GRGCYC 1 cut(s) 660
Fsp4HI GCNGC 3 cut(s) 651, 682, 795
FspBI CTAG 2 cut(s) 624, 825
GluI GCNGC 3 cut(s) 651, 682, 795
GsaI CCCAGC 1 cut(s) 657
GsuI CTGGAG 2 cut(s) 230, 366
HaeIII GGCC 2 cut(s) 467, 912
HapII CCGG 2 cut(s) 134, 452
Hin1II CATG 6 cut(s) 82, 124, 298, 395, 517, 728
HindIII AAGCTT 2 cut(s) 559, 772
HinfI GANTC 3 cut(s) 281, 778, 900
HpaII CCGG 2 cut(s) 134, 452
HphI GGTGA 3 cut(s) 281, 628, 1069
Hpy188I TCNGA 5 cut(s) 25, 187, 480, 1012, 1071
Hpy188III TCNNGA 5 cut(s) 134, 392, 687, 737, 819
HpyAV CCTTC 3 cut(s) 158, 377, 822
HpyCH4III ACNGT 3 cut(s) 644, 677, 1046
HpyCH4IV ACGT 1 cut(s) 1019
HpyCH4V TGCA 5 cut(s) 431, 497, 794, 863, 907
HpyF10VI GCNNNNNNNGC 2 cut(s) 137, 678
HpyF3I CTNAG 8 cut(s) 44, 63, 562, 685, 735, 743, 896, 1057
HpySE526I ACGT 1 cut(s) 1019
Hsp92II CATG 6 cut(s) 82, 124, 298, 395, 517, 728
Kpn2I TCCGGA 1 cut(s) 133
Ksp22I TGATCA 3 cut(s) 354, 394, 694
Kzo9I GATC 6 cut(s) 354, 388, 394, 694, 844, 1066
LmnI GCTCC 4 cut(s) 4, 136, 739, 1036
Lsp1109I GCAGC 2 cut(s) 668, 806
LweI GCATC 1 cut(s) 127
MaeI CTAG 2 cut(s) 624, 825
MaeII ACGT 1 cut(s) 1019
MaeIII GTNAC 3 cut(s) 266, 301, 616
MalI GATC 6 cut(s) 356, 390, 396, 696, 846, 1068
MboI GATC 6 cut(s) 354, 388, 394, 694, 844, 1066
MboII GAAGA 5 cut(s) 276, 446, 647, 650, 885
MhlI GDGCHC 1 cut(s) 660
MluCI AATT 9 cut(s) 98, 172, 202, 217, 367, 522, 538, 730, 977
MlyI GAGTC 2 cut(s) 787, 909
MmeI TCCRAC 1 cut(s) 806
MroI TCCGGA 1 cut(s) 133
MroXI GAANNNNTTC 2 cut(s) 100, 570
MseI TTAA 2 cut(s) 525, 1004
MslI CAYNNNNRTG 1 cut(s) 723
MspA1I CMGCKG 1 cut(s) 653
MspI CCGG 2 cut(s) 134, 452
MspR9I CCNGG 1 cut(s) 452
MwoI GCNNNNNNNGC 2 cut(s) 137, 678
NciI CCSGG 1 cut(s) 452
NcoI CCATGG 1 cut(s) 513
NdeII GATC 6 cut(s) 354, 388, 394, 694, 844, 1066
NheI GCTAGC 1 cut(s) 623
NlaIII CATG 6 cut(s) 82, 124, 298, 395, 517, 728
NmuCI GTSAC 1 cut(s) 616
PagI TCATGA 1 cut(s) 391
PaqCI CACCTGC 1 cut(s) 604
PdmI GAANNNNTTC 2 cut(s) 100, 570
PfeI GAWTC 1 cut(s) 281
PkrI GCNGC 3 cut(s) 652, 683, 796
PleI GAGTC 2 cut(s) 786, 908
PpsI GAGTC 2 cut(s) 786, 908
Ppu21I YACGTR 1 cut(s) 1020
PspFI CCCAGC 1 cut(s) 653
PspPI GGNCC 3 cut(s) 36, 466, 910
PstI CTGCAG 2 cut(s) 499, 909
PstNI CAGNNNCTG 3 cut(s) 681, 742, 842
RsaI GTAC 1 cut(s) 56
RsaNI GTAC 1 cut(s) 55
RseI CAYNNNNRTG 1 cut(s) 723
SaqAI TTAA 2 cut(s) 525, 1004
SatI GCNGC 3 cut(s) 651, 682, 795
Sau3AI GATC 6 cut(s) 354, 388, 394, 694, 844, 1066
Sau96I GGNCC 3 cut(s) 36, 466, 910
SbfI CCTGCAGG 1 cut(s) 909
SchI GAGTC 2 cut(s) 787, 909
ScrFI CCNGG 1 cut(s) 452
SdaI CCTGCAGG 1 cut(s) 909
SduI GDGCHC 1 cut(s) 660
SfaNI GCATC 1 cut(s) 127
SfcI CTRYAG 3 cut(s) 495, 673, 905
SinI GGWCC 1 cut(s) 36
SmiMI CAYNNNNRTG 1 cut(s) 723
SmlI CTYRAG 4 cut(s) 274, 775, 805, 953
SmoI CTYRAG 4 cut(s) 274, 775, 805, 953
Sse8387I CCTGCAGG 1 cut(s) 909
Sse9I AATT 9 cut(s) 98, 172, 202, 217, 367, 522, 538, 730, 977
SsiI CCGC 2 cut(s) 455, 651
SspI AATATT 1 cut(s) 706
SspMI CTAG 2 cut(s) 624, 825
StyD4I CCNGG 1 cut(s) 450
StyI CCWWGG 2 cut(s) 469, 513
TaaI ACNGT 3 cut(s) 644, 677, 1046
TaiI ACGT 1 cut(s) 1022
TasI AATT 9 cut(s) 98, 172, 202, 217, 367, 522, 538, 730, 977
TatI WGTACW 1 cut(s) 54
TauI GCSGC 1 cut(s) 653
TfiI GAWTC 1 cut(s) 281
Tru1I TTAA 2 cut(s) 525, 1004
Tru9I TTAA 2 cut(s) 525, 1004
TscAI CASTG 5 cut(s) 52, 213, 265, 847, 1051
TseFI GTSAC 1 cut(s) 616
TseI GCWGC 2 cut(s) 681, 794
Tsp45I GTSAC 1 cut(s) 616
TspDTI ATGAA 7 cut(s) 67, 171, 177, 531, 593, 648, 1067
TspRI CASTG 5 cut(s) 52, 213, 265, 847, 1051
VpaK11BI GGWCC 1 cut(s) 36
XapI RAATTY 6 cut(s) 98, 202, 367, 538, 730, 977
XmnI GAANNNNTTC 2 cut(s) 100, 570
XspI CTAG 2 cut(s) 624, 825
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.