FvH4_4g05902

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0002575
Physical Location & Seq
Forward (+)
5329543 .. 5331907
2365 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g05902.t1

Sequence Viewer

Length: 1020 bp
ATGGATCGCACCGGAGCTGTTCCAGAGGGAGAATGGAGCTCTCACTCTCTTGGTGGGATGTTCACTGGTGATCAGCAGGAGGCAGATTTCATGATGATCCAGTTGCTTGAGAATTACCCAAATATTCCAGACATGGCAATCCCGGATGATTTTTGGCCTGGCAATGAAGAATCAACATTGATGAATATTTCAAGCAACTATGGGAGTCCAGATTATTCTTTAGAAAGTACTTACTCTCTTTTGAACGACAGTTCACAAGAGACTGATCTATTTTCCACTTCAACCGATCGATTCTACAACTTGAGCGGTTCTCGCCCAATGACTTCGGTAACAAGGAATGTTAACGGCAACGACAATTCTATATCTAATGACTTCTGTGCTGAGGAGGCGATAGACGTCACTAGTTTGTATCTCATTCAAGAAGAAGGTGATGTGAGCATTGACGGCAATGTGGAAGAGCCCAGCAGGTACCAGCGTGATGAACCAGCCAGCATTGATCATGAAAAAAGTAACACCAGCAGCTCCAAGATTAGTTCTATGAAGAGACCAAAAGCCTCCGAAAATGATCAAATGAATAAGAGGAGCAGAAAGTCTAAGAAGAGCTGCAACAACGAAGAAGATGGGAATCATATTGATAATGCTTCTCAGCTTCAGCCTAGTGGAGGGATTACTGAGGCTGCATCTCTCAACTTGAATGGAAAGACAAGAGCTACTAGGGGGTCTGCCACCGATGCCCAAAGTGTCTATGCAAGGAGAAGAAGAGAGCGAATAAACGAAAGGCTCAAAGTCCTGCAAAACCTTGTCCCCAATGGAAGAAAGGTTGATATTAGCACAATGCTTGAGGAAGCTGTCAATTATGTGAAGTTCTTGCAACTCCAAATTAAGATGTTAAGCTCGGACGATATGTGGATGTATGCTCCCATTTGTTACAATGGAATGTTCTTCGGGCTTGATGGAGATGAGTCTTCCCACGCTAGATCAATGTTAGAATTGAAAGATAACATACAAAAGAGATTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000902 GO:0000904 GO:0000976 GO:0000981 GO:0001046 GO:0001047 GO:0001067 GO:0001228 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009888 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010015 GO:0010033 GO:0010053 GO:0010054 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016043 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0021700 GO:0022622 GO:0030154 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032501 GO:0032502 GO:0032774 GO:0032989 GO:0034641 GO:0034645 GO:0034654 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048364 GO:0048468 GO:0048469 GO:0048518 GO:0048522 GO:0048731 GO:0048764 GO:0048765 GO:0048766 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0060255 GO:0065007 GO:0071695 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0090558 GO:0090627 GO:0097159 GO:0097659 GO:0099402 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:1905392 GO:1990837 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HLH

Protein Analysis

340

Amino Acids

38.02

Weight (kDa)

4.71

Isoelectric Point (pI)

55.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 251 - 291 1.2e-09 Helix-loop-helix DNA-binding domain
bHLH_LHW PF23176 252 - 291 2.3e-06 Transcription factor LHW bHLH domain
bHLH_SAC51 PF23173 252 - 295 3.3e-06 Transcription factor SAC51 bHLH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 399
Acc36I ACCTGC 1 cut(s) 456
Acc65I GGTACC 1 cut(s) 468
AccB1I GGYRCC 1 cut(s) 468
AccBSI CCGCTC 1 cut(s) 306
AciI CCGC 1 cut(s) 306
AclWI GGATC 2 cut(s) 12, 91
AcuI CTGAAG 1 cut(s) 635
AcyI GRCGYC 1 cut(s) 396
AfaI GTAC 2 cut(s) 229, 470
AfiI CCNNNNNNNGG 1 cut(s) 662
AgsI TTSAA 6 cut(s) 192, 244, 282, 419, 694, 994
AhlI ACTAGT 1 cut(s) 401
AjnI CCWGG 1 cut(s) 157
AluBI AGCT 8 cut(s) 17, 39, 522, 603, 649, 710, 848, 894
AluI AGCT 8 cut(s) 17, 39, 522, 603, 649, 710, 848, 894
Alw21I GWGCWC 1 cut(s) 41
Alw26I GTCTC 2 cut(s) 254, 538
AlwI GGATC 2 cut(s) 12, 91
AoxI GGCC 1 cut(s) 155
ApeKI GCWGC 3 cut(s) 519, 603, 677
Asp718I GGTACC 1 cut(s) 468
AsuC2I CCSGG 1 cut(s) 143
AsuHPI GGTGA 2 cut(s) 80, 440
BaeI ACNNNNGTAYC 2 cut(s) 460, 493
BanI GGYRCC 1 cut(s) 468
BanII GRGCYC 2 cut(s) 41, 462
BbsI GAAGAC 1 cut(s) 957
Bbv12I GWGCWC 1 cut(s) 41
BbvCI CCTCAGC 1 cut(s) 381
BbvI GCAGC 3 cut(s) 531, 590, 664
BccI CCATC 2 cut(s) 614, 947
BceAI ACGGC 2 cut(s) 361, 460
BciT130I CCWGG 1 cut(s) 159
BclI TGATCA 3 cut(s) 70, 496, 565
BcnI CCSGG 1 cut(s) 143
BcoDI GTCTC 2 cut(s) 254, 538
BcuI ACTAGT 1 cut(s) 401
BfaI CTAG 4 cut(s) 402, 657, 714, 975
BfuAI ACCTGC 1 cut(s) 456
BisI GCNGC 3 cut(s) 520, 604, 678
BlsI GCNGC 3 cut(s) 521, 605, 679
BmcAI AGTACT 1 cut(s) 229
Bme1390I CCNGG 2 cut(s) 143, 159
BmiI GGNNCC 1 cut(s) 470
BmrFI CCNGG 2 cut(s) 143, 159
BmsI GCATC 2 cut(s) 689, 721
BpiI GAAGAC 1 cut(s) 957
BplI GAGNNNNNCTC 2 cut(s) 295, 327
Bpu10I CCTNAGC 1 cut(s) 381
BpuEI CTTGAG 3 cut(s) 128, 322, 860
BpuMI CCSGG 1 cut(s) 143
Bsa29I ATCGAT 1 cut(s) 289
BsaBI GATNNNNATC 1 cut(s) 624
BsaHI GRCGYC 1 cut(s) 396
BsaI GGTCTC 1 cut(s) 538
BsaWI WCCGGW 1 cut(s) 11
Bsc4I CCNNNNNNNGG 1 cut(s) 662
Bse1I ACTGG 2 cut(s) 70, 100
Bse3DI GCAATG 2 cut(s) 169, 454
Bse8I GATNNNNATC 1 cut(s) 624
BseBI CCWGG 1 cut(s) 159
BseCI ATCGAT 1 cut(s) 289
BseGI GGATG 3 cut(s) 63, 151, 915
BseJI GATNNNNATC 1 cut(s) 624
BseLI CCNNNNNNNGG 1 cut(s) 662
BseMI GCAATG 2 cut(s) 169, 454
BseMII CTCAG 3 cut(s) 372, 659, 663
BseNI ACTGG 2 cut(s) 70, 100
BseRI GAGGAG 2 cut(s) 398, 595
BseXI GCAGC 3 cut(s) 531, 590, 664
BseYI CCCAGC 1 cut(s) 461
Bsh1285I CGRYCG 1 cut(s) 289
BshFI GGCC 1 cut(s) 157
BshNI GGYRCC 1 cut(s) 468
BshVI ATCGAT 1 cut(s) 289
BsiEI CGRYCG 1 cut(s) 289
BsiHKAI GWGCWC 1 cut(s) 41
BsiSI CCGG 2 cut(s) 12, 143
BslFI GGGAC 1 cut(s) 788
BslI CCNNNNNNNGG 1 cut(s) 662
BsmAI GTCTC 2 cut(s) 254, 538
BsmFI GGGAC 1 cut(s) 788
BsnI GGCC 1 cut(s) 157
Bso31I GGTCTC 1 cut(s) 538
Bsp1286I GDGCHC 2 cut(s) 41, 462
Bsp143I GATC 8 cut(s) 4, 70, 96, 265, 286, 496, 565, 977
BspACI CCGC 1 cut(s) 306
BspANI GGCC 1 cut(s) 157
BspCNI CTCAG 3 cut(s) 373, 658, 664
BspDI ATCGAT 1 cut(s) 289
BspHI TCATGA 2 cut(s) 90, 499
BspLI GGNNCC 1 cut(s) 470
BspMI ACCTGC 1 cut(s) 456
BspPI GGATC 2 cut(s) 12, 91
BspQI GCTCTTC 2 cut(s) 450, 593
BspT107I GGYRCC 1 cut(s) 468
BspTNI GGTCTC 1 cut(s) 538
BsrBI CCGCTC 1 cut(s) 306
BsrDI GCAATG 2 cut(s) 169, 454
BsrI ACTGG 2 cut(s) 70, 100
BssMI GATC 8 cut(s) 4, 70, 96, 265, 286, 496, 565, 977
BssNI GRCGYC 1 cut(s) 396
Bst2UI CCWGG 1 cut(s) 159
Bst4CI ACNGT 1 cut(s) 251
Bst6I CTCTTC 4 cut(s) 450, 536, 593, 754
BstACI GRCGYC 1 cut(s) 396
BstC8I GCNNGC 1 cut(s) 490
BstDEI CTNAG 4 cut(s) 381, 594, 645, 672
BstENI CCTNNNNNAGG 1 cut(s) 660
BstF5I GGATG 3 cut(s) 63, 151, 915
BstKTI GATC 8 cut(s) 7, 73, 99, 268, 289, 499, 568, 980
BstMAI GTCTC 2 cut(s) 254, 538
BstMBI GATC 8 cut(s) 4, 70, 96, 265, 286, 496, 565, 977
BstMCI CGRYCG 1 cut(s) 289
BstMWI GCNNNNNNNGC 4 cut(s) 312, 386, 444, 731
BstNI CCWGG 1 cut(s) 159
BstSCI CCNGG 2 cut(s) 141, 157
BstV1I GCAGC 3 cut(s) 531, 590, 664
BstV2I GAAGAC 1 cut(s) 957
Bsu15I ATCGAT 1 cut(s) 289
BsuRI GGCC 1 cut(s) 157
BsuTUI ATCGAT 1 cut(s) 289
BtsCI GGATG 3 cut(s) 63, 151, 915
BtsIMutI CAGTG 1 cut(s) 63
BveI ACCTGC 1 cut(s) 456
Cac8I GCNNGC 1 cut(s) 490
CciI TCATGA 2 cut(s) 90, 499
ClaI ATCGAT 1 cut(s) 289
Csp6I GTAC 2 cut(s) 228, 469
CviAII CATG 3 cut(s) 91, 133, 500
CviQI GTAC 2 cut(s) 228, 469
DdeI CTNAG 4 cut(s) 381, 594, 645, 672
DpnI GATC 8 cut(s) 6, 72, 98, 267, 288, 498, 567, 979
DpnII GATC 8 cut(s) 4, 70, 96, 265, 286, 496, 565, 977
Eam1104I CTCTTC 4 cut(s) 450, 536, 593, 754
EarI CTCTTC 4 cut(s) 450, 536, 593, 754
Ecl136II GAGCTC 1 cut(s) 39
Eco24I GRGCYC 2 cut(s) 41, 462
Eco31I GGTCTC 1 cut(s) 538
Eco53kI GAGCTC 1 cut(s) 39
Eco57I CTGAAG 1 cut(s) 635
EcoICRI GAGCTC 1 cut(s) 39
EcoNI CCTNNNNNAGG 1 cut(s) 660
EcoRII CCWGG 1 cut(s) 157
EcoT38I GRGCYC 2 cut(s) 41, 462
FaeI CATG 3 cut(s) 94, 136, 503
FaqI GGGAC 1 cut(s) 788
FatI CATG 3 cut(s) 90, 132, 499
FbaI TGATCA 3 cut(s) 70, 496, 565
Fnu4HI GCNGC 3 cut(s) 520, 604, 678
FokI GGATG 3 cut(s) 70, 158, 922
FriOI GRGCYC 2 cut(s) 41, 462
Fsp4HI GCNGC 3 cut(s) 520, 604, 678
FspBI CTAG 4 cut(s) 402, 657, 714, 975
GluI GCNGC 3 cut(s) 520, 604, 678
GsaI CCCAGC 1 cut(s) 465
HaeIII GGCC 1 cut(s) 157
HapII CCGG 2 cut(s) 12, 143
Hin1I GRCGYC 1 cut(s) 396
Hin1II CATG 3 cut(s) 94, 136, 503
HincII GTYRAC 1 cut(s) 343
HindII GTYRAC 1 cut(s) 343
HinfI GANTC 5 cut(s) 170, 205, 291, 625, 962
HpaI GTTAAC 1 cut(s) 343
HpaII CCGG 2 cut(s) 12, 143
HphI GGTGA 2 cut(s) 80, 440
Hpy166II GTNNAC 3 cut(s) 63, 254, 343
Hpy188I TCNGA 2 cut(s) 559, 898
Hpy188III TCNNGA 6 cut(s) 23, 91, 128, 209, 419, 500
Hpy8I GTNNAC 3 cut(s) 63, 254, 343
HpyAV CCTTC 1 cut(s) 419
HpyCH4III ACNGT 1 cut(s) 251
HpyCH4IV ACGT 1 cut(s) 396
HpyCH4V TGCA 5 cut(s) 606, 680, 749, 793, 871
HpyF10VI GCNNNNNNNGC 4 cut(s) 312, 386, 444, 731
HpyF3I CTNAG 4 cut(s) 381, 594, 645, 672
HpySE526I ACGT 1 cut(s) 396
Hsp92I GRCGYC 1 cut(s) 396
Hsp92II CATG 3 cut(s) 94, 136, 503
KpnI GGTACC 1 cut(s) 472
Ksp22I TGATCA 3 cut(s) 70, 496, 565
KspAI GTTAAC 1 cut(s) 343
Kzo9I GATC 8 cut(s) 4, 70, 96, 265, 286, 496, 565, 977
LguI GCTCTTC 2 cut(s) 450, 593
LmnI GCTCC 5 cut(s) 14, 36, 527, 582, 922
Lsp1109I GCAGC 3 cut(s) 531, 590, 664
LweI GCATC 2 cut(s) 689, 721
MaeI CTAG 4 cut(s) 402, 657, 714, 975
MaeII ACGT 1 cut(s) 396
MaeIII GTNAC 4 cut(s) 328, 397, 509, 926
MalI GATC 8 cut(s) 6, 72, 98, 267, 288, 498, 567, 979
MbiI CCGCTC 1 cut(s) 306
MboI GATC 8 cut(s) 4, 70, 96, 265, 286, 496, 565, 977
MhlI GDGCHC 2 cut(s) 41, 462
MluCI AATT 5 cut(s) 112, 355, 853, 879, 989
MlyI GAGTC 2 cut(s) 214, 971
MnlI CCTC 9 cut(s) 19, 73, 376, 379, 565, 573, 656, 667, 835
MseI TTAA 3 cut(s) 342, 882, 890
MspI CCGG 2 cut(s) 12, 143
MspR9I CCNGG 2 cut(s) 143, 159
MvaI CCWGG 1 cut(s) 159
MwoI GCNNNNNNNGC 4 cut(s) 312, 386, 444, 731
NciI CCSGG 1 cut(s) 143
NdeII GATC 8 cut(s) 4, 70, 96, 265, 286, 496, 565, 977
NlaIII CATG 3 cut(s) 94, 136, 503
NlaIV GGNNCC 1 cut(s) 470
NmuCI GTSAC 1 cut(s) 397
PagI TCATGA 2 cut(s) 90, 499
PciSI GCTCTTC 2 cut(s) 450, 593
PfeI GAWTC 3 cut(s) 170, 291, 625
PfoI TCCNGGA 1 cut(s) 141
PkrI GCNGC 3 cut(s) 521, 605, 679
Ple19I CGATCG 1 cut(s) 289
PleI GAGTC 2 cut(s) 213, 970
PpsI GAGTC 2 cut(s) 213, 970
Psp124BI GAGCTC 1 cut(s) 41
Psp6I CCWGG 1 cut(s) 157
PspFI CCCAGC 1 cut(s) 461
PspGI CCWGG 1 cut(s) 157
PspN4I GGNNCC 1 cut(s) 470
PvuI CGATCG 1 cut(s) 289
RsaI GTAC 2 cut(s) 229, 470
RsaNI GTAC 2 cut(s) 228, 469
SacI GAGCTC 1 cut(s) 41
SapI GCTCTTC 2 cut(s) 450, 593
SaqAI TTAA 3 cut(s) 342, 882, 890
SatI GCNGC 3 cut(s) 520, 604, 678
Sau3AI GATC 8 cut(s) 4, 70, 96, 265, 286, 496, 565, 977
ScaI AGTACT 1 cut(s) 229
SchI GAGTC 2 cut(s) 214, 971
ScrFI CCNGG 2 cut(s) 143, 159
SduI GDGCHC 2 cut(s) 41, 462
SfaNI GCATC 2 cut(s) 689, 721
SmlI CTYRAG 3 cut(s) 107, 301, 839
SmoI CTYRAG 3 cut(s) 107, 301, 839
SpeI ACTAGT 1 cut(s) 401
Sse9I AATT 5 cut(s) 112, 355, 853, 879, 989
SsiI CCGC 1 cut(s) 306
SspI AATATT 2 cut(s) 124, 187
SspMI CTAG 4 cut(s) 402, 657, 714, 975
SstI GAGCTC 1 cut(s) 41
StyD4I CCNGG 2 cut(s) 141, 157
TaaI ACNGT 1 cut(s) 251
TaiI ACGT 1 cut(s) 399
TaqI TCGA 1 cut(s) 289
TasI AATT 5 cut(s) 112, 355, 853, 879, 989
TatI WGTACW 1 cut(s) 227
TfiI GAWTC 3 cut(s) 170, 291, 625
Tru1I TTAA 3 cut(s) 342, 882, 890
Tru9I TTAA 3 cut(s) 342, 882, 890
TscAI CASTG 1 cut(s) 70
TseFI GTSAC 1 cut(s) 397
TseI GCWGC 3 cut(s) 519, 603, 677
Tsp45I GTSAC 1 cut(s) 397
TspDTI ATGAA 7 cut(s) 79, 180, 197, 495, 516, 554, 587
TspRI CASTG 1 cut(s) 70
XagI CCTNNNNNAGG 1 cut(s) 660
XcmI CCANNNNNNNNNTGG 1 cut(s) 30
XspI CTAG 4 cut(s) 402, 657, 714, 975
ZraI GACGTC 1 cut(s) 397
ZrmI AGTACT 1 cut(s) 229
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.