MD05G1098900.v1.1

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0002575
Physical Location & Seq
Forward (+)
20593122 .. 20595522
2401 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1098900.v1.1.491

Sequence Viewer

Length: 1092 bp
ATGGAGGATATTGGAGCTGTTTCAGAGGGAGAATGGACCTCTCTCAGCGGGATGTACACTGCTGAGGAGGTAGATTTCATGTCCCAGTTGCTTGGAAATTCTTCAAACCAGACATCAAGCATGGGAATTCCAGAGGCTTCTTGGCCTGGAGGCTACTATGAAGGTTCACATTATTATTCATCAGAAACTTCTGATTCAAACTCACAAGAGGCTAATTTGAGTGGGGGTACTAGGACTTTTTACCCCACTTCAAGCAATGAGAGTTACTACTTGAATGAATCTTCTCAACCCATGTTGGCAACCAACAATAATAACATCTCTATGTCTATTGATTTTGGTGCTGGGGATGTGATCAAAACCCTAAATTCCTATCTCATTGAAGGAGATCATGATCAATGCTTGAACCAAGAAACAAGTGAAGGCAATCCAGAAGAGCGTGGCGGAAACCCGGCTGAAGCTGTGGCTCTTGGAAATCAGAAATTTGATATGACTGCTGCACAAGAACAATCCATGGAGGAAATTAACCCCACCAAGAATTCATTGAAAAGATCTCAAAGCTTAGAAAATGTTCAAATGAAAAAGATGAACATAAAGGCAAAGAAGAGCCGGCAGGTGACAACTAGCATCAATGAAGAAGACTGTACTACCGCCGGGCTCAACAGACAGAGCTCCAGTTGCTGCTCAGGAGAAGATCAGTCCAGTGTTGCCTCACATTCCCATGAGAATTCTCAGGAGCTGAGTGGAGGGGTTAGTGTTACTTCAACTTCAAGCTTGAGTCCAACAGTTGCATCTCTCAACTTGAGTGGCAAATCAAGAGCTAGAAGGGGGTCAGCCACTGATCCCCAAAGCCTCTATGCAAGGAAAAGAAGAGAGAAAATGAATGAAAGGCTGAGAGCCCTGCAGGGCCTTGTGCCCAACGGAACAAAGGTTGATATTAGCACAATGCTTGAGGAAGCTGTCCATTATGTGAAGTTTTTACAACTCCAAATTAAGCTGTTAAGCTCTGATGATATGTGGATGTATGCTCCCATCTGTTACAATGGAATGGACCTGGGGCTTGATCCGAAGCTCACCACACCACAGCAATCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000902 GO:0000904 GO:0000976 GO:0000981 GO:0001046 GO:0001047 GO:0001067 GO:0001228 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009888 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010015 GO:0010033 GO:0010053 GO:0010054 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016043 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0021700 GO:0022622 GO:0030154 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032501 GO:0032502 GO:0032774 GO:0032989 GO:0034641 GO:0034645 GO:0034654 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048364 GO:0048468 GO:0048469 GO:0048518 GO:0048522 GO:0048731 GO:0048764 GO:0048765 GO:0048766 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0060255 GO:0065007 GO:0071695 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0090558 GO:0090627 GO:0097159 GO:0097659 GO:0099402 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:1905392 GO:1990837 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HLH

Protein Analysis

364

Amino Acids

39.6

Weight (kDa)

4.77

Isoelectric Point (pI)

64.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 287 - 327 3e-08 Helix-loop-helix DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 601
Acc36I ACCTGC 1 cut(s) 601
AciI CCGC 3 cut(s) 48, 441, 648
AclWI GGATC 2 cut(s) 833, 1055
AcsI RAATTY 6 cut(s) 97, 126, 364, 479, 535, 724
AcuI CTGAAG 1 cut(s) 474
AfaI GTAC 3 cut(s) 56, 229, 643
AjnI CCWGG 2 cut(s) 145, 1050
Alw21I GWGCWC 1 cut(s) 671
AlwI GGATC 2 cut(s) 833, 1055
AlwNI CAGNNNCTG 3 cut(s) 678, 736, 836
AoxI GGCC 2 cut(s) 143, 904
ApeKI GCWGC 2 cut(s) 494, 678
ApoI RAATTY 6 cut(s) 97, 126, 364, 479, 535, 724
Asp700I GAANNNNTTC 2 cut(s) 100, 567
AspS9I GGNCC 3 cut(s) 36, 904, 1048
AsuC2I CCSGG 2 cut(s) 449, 652
AsuHPI GGTGA 2 cut(s) 625, 1063
AvaII GGWCC 2 cut(s) 36, 1048
BaeGI GKGCMC 1 cut(s) 915
BanII GRGCYC 3 cut(s) 657, 671, 898
BbsI GAAGAC 1 cut(s) 642
Bbv12I GWGCWC 1 cut(s) 671
BbvCI CCTCAGC 1 cut(s) 63
BbvI GCAGC 2 cut(s) 481, 665
BccI CCATC 1 cut(s) 1037
BciT130I CCWGG 2 cut(s) 147, 1052
BclI TGATCA 2 cut(s) 351, 391
BcnI CCSGG 2 cut(s) 449, 652
BfaI CTAG 3 cut(s) 231, 621, 819
BfmI CTRYAG 1 cut(s) 899
BfuAI ACCTGC 1 cut(s) 601
BglII AGATCT 1 cut(s) 548
BisI GCNGC 2 cut(s) 495, 679
BlsI GCNGC 2 cut(s) 496, 680
Bme1390I CCNGG 4 cut(s) 147, 449, 652, 1052
Bme18I GGWCC 2 cut(s) 36, 1048
BmgT120I GGNCC 3 cut(s) 36, 904, 1048
BmrFI CCNGG 4 cut(s) 147, 449, 652, 1052
BmrI ACTGGG 1 cut(s) 79
BmsI GCATC 2 cut(s) 633, 797
BmuI ACTGGG 1 cut(s) 79
BpiI GAAGAC 1 cut(s) 642
BpmI CTGGAG 2 cut(s) 168, 655
Bpu10I CCTNAGC 2 cut(s) 63, 682
BpuEI CTTGAG 3 cut(s) 793, 820, 968
BpuMI CCSGG 2 cut(s) 449, 652
BsaBI GATNNNNATC 1 cut(s) 390
BsaJI CCNNGG 2 cut(s) 510, 1051
Bse118I RCCGGY 1 cut(s) 606
Bse1I ACTGG 3 cut(s) 85, 672, 699
Bse3DI GCAATG 1 cut(s) 262
Bse8I GATNNNNATC 1 cut(s) 390
BseBI CCWGG 2 cut(s) 147, 1052
BseDI CCNNGG 2 cut(s) 510, 1051
BseGI GGATG 3 cut(s) 57, 352, 1023
BseJI GATNNNNATC 1 cut(s) 390
BseMI GCAATG 1 cut(s) 262
BseMII CTCAG 6 cut(s) 54, 58, 696, 728, 743, 881
BseNI ACTGG 3 cut(s) 85, 672, 699
BseRI GAGGAG 1 cut(s) 80
BseSI GKGCMC 1 cut(s) 915
BseXI GCAGC 2 cut(s) 481, 665
BseYI CCCAGC 1 cut(s) 341
BsgI GTGCAG 1 cut(s) 480
BshFI GGCC 2 cut(s) 145, 906
BsiHKAI GWGCWC 1 cut(s) 671
BsiSI CCGG 3 cut(s) 449, 607, 651
BslFI GGGAC 1 cut(s) 67
BsmFI GGGAC 1 cut(s) 67
BsnI GGCC 2 cut(s) 145, 906
Bsp1286I GDGCHC 4 cut(s) 657, 671, 898, 915
Bsp1407I TGTACA 1 cut(s) 54
Bsp143I GATC 7 cut(s) 351, 385, 391, 548, 691, 838, 1060
Bsp19I CCATGG 1 cut(s) 510
BspACI CCGC 3 cut(s) 48, 441, 648
BspANI GGCC 2 cut(s) 145, 906
BspCNI CTCAG 6 cut(s) 55, 57, 695, 729, 742, 882
BspHI TCATGA 1 cut(s) 388
BspMAI CTGCAG 1 cut(s) 903
BspMI ACCTGC 1 cut(s) 601
BspPI GGATC 2 cut(s) 833, 1055
BspQI GCTCTTC 2 cut(s) 426, 596
BsrDI GCAATG 1 cut(s) 262
BsrFI RCCGGY 1 cut(s) 606
BsrGI TGTACA 1 cut(s) 54
BsrI ACTGG 3 cut(s) 85, 672, 699
BssAI RCCGGY 1 cut(s) 606
BssECI CCNNGG 2 cut(s) 510, 1051
BssMI GATC 7 cut(s) 351, 385, 391, 548, 691, 838, 1060
BssT1I CCWWGG 1 cut(s) 510
Bst2UI CCWGG 2 cut(s) 147, 1052
Bst4CI ACNGT 2 cut(s) 641, 784
Bst6I CTCTTC 3 cut(s) 426, 596, 862
BstAUI TGTACA 1 cut(s) 54
BstC8I GCNNGC 1 cut(s) 608
BstDEI CTNAG 7 cut(s) 44, 63, 559, 682, 729, 737, 890
BstDSI CCRYGG 1 cut(s) 510
BstF5I GGATG 3 cut(s) 57, 352, 1023
BstKTI GATC 7 cut(s) 354, 388, 394, 551, 694, 841, 1063
BstMBI GATC 7 cut(s) 351, 385, 391, 548, 691, 838, 1060
BstMWI GCNNNNNNNGC 1 cut(s) 675
BstNI CCWGG 2 cut(s) 147, 1052
BstSCI CCNGG 4 cut(s) 145, 447, 650, 1050
BstSFI CTRYAG 1 cut(s) 899
BstSLI GKGCMC 1 cut(s) 915
BstV1I GCAGC 2 cut(s) 481, 665
BstV2I GAAGAC 1 cut(s) 642
BstX2I RGATCY 1 cut(s) 548
BstXI CCANNNNNNTGG 1 cut(s) 92
BstYI RGATCY 1 cut(s) 548
BsuRI GGCC 2 cut(s) 145, 906
BtgI CCRYGG 1 cut(s) 510
BtsCI GGATG 3 cut(s) 57, 352, 1023
BtsI GCAGTG 1 cut(s) 57
BtsIMutI CAGTG 3 cut(s) 57, 706, 834
BveI ACCTGC 1 cut(s) 601
Cac8I GCNNGC 1 cut(s) 608
CaiI CAGNNNCTG 3 cut(s) 678, 736, 836
CciI TCATGA 1 cut(s) 388
Cfr10I RCCGGY 1 cut(s) 606
Cfr13I GGNCC 3 cut(s) 36, 904, 1048
Csp6I GTAC 3 cut(s) 55, 228, 642
CspCI CAANNNNNGTGG 2 cut(s) 784, 819
CviAII CATG 6 cut(s) 79, 121, 292, 389, 511, 719
CviQI GTAC 3 cut(s) 55, 228, 642
DdeI CTNAG 7 cut(s) 44, 63, 559, 682, 729, 737, 890
DpnI GATC 7 cut(s) 353, 387, 393, 550, 693, 840, 1062
DpnII GATC 7 cut(s) 351, 385, 391, 548, 691, 838, 1060
Eam1104I CTCTTC 3 cut(s) 426, 596, 862
EarI CTCTTC 3 cut(s) 426, 596, 862
EciI GGCGGA 1 cut(s) 456
Ecl136II GAGCTC 1 cut(s) 669
Eco130I CCWWGG 1 cut(s) 510
Eco24I GRGCYC 3 cut(s) 657, 671, 898
Eco47I GGWCC 2 cut(s) 36, 1048
Eco53kI GAGCTC 1 cut(s) 669
Eco57I CTGAAG 1 cut(s) 474
EcoICRI GAGCTC 1 cut(s) 669
EcoO109I RGGNCCY 1 cut(s) 904
EcoRI GAATTC 3 cut(s) 126, 535, 724
EcoRII CCWGG 2 cut(s) 145, 1050
EcoT14I CCWWGG 1 cut(s) 510
EcoT38I GRGCYC 3 cut(s) 657, 671, 898
ErhI CCWWGG 1 cut(s) 510
FaeI CATG 6 cut(s) 82, 124, 295, 392, 514, 722
FaqI GGGAC 1 cut(s) 67
FatI CATG 6 cut(s) 78, 120, 291, 388, 510, 718
FauI CCCGC 1 cut(s) 41
FbaI TGATCA 2 cut(s) 351, 391
Fnu4HI GCNGC 2 cut(s) 495, 679
FokI GGATG 3 cut(s) 64, 359, 1030
FriOI GRGCYC 3 cut(s) 657, 671, 898
Fsp4HI GCNGC 2 cut(s) 495, 679
FspBI CTAG 3 cut(s) 231, 621, 819
GluI GCNGC 2 cut(s) 495, 679
GsaI CCCAGC 1 cut(s) 345
GsuI CTGGAG 2 cut(s) 168, 655
HaeIII GGCC 2 cut(s) 145, 906
HapII CCGG 3 cut(s) 449, 607, 651
Hin1II CATG 6 cut(s) 82, 124, 295, 392, 514, 722
HindIII AAGCTT 2 cut(s) 556, 769
HinfI GANTC 3 cut(s) 194, 278, 775
HpaII CCGG 3 cut(s) 449, 607, 651
HphI GGTGA 2 cut(s) 625, 1063
Hpy166II GTNNAC 2 cut(s) 57, 167
Hpy188I TCNGA 6 cut(s) 25, 184, 193, 477, 1006, 1065
Hpy188III TCNNGA 6 cut(s) 131, 389, 428, 684, 731, 813
Hpy8I GTNNAC 2 cut(s) 57, 167
HpyAV CCTTC 4 cut(s) 155, 374, 413, 816
HpyCH4III ACNGT 2 cut(s) 641, 784
HpyCH4V TGCA 4 cut(s) 497, 788, 857, 901
HpyF10VI GCNNNNNNNGC 1 cut(s) 675
HpyF3I CTNAG 7 cut(s) 44, 63, 559, 682, 729, 737, 890
Hsp92II CATG 6 cut(s) 82, 124, 295, 392, 514, 722
KroI GCCGGC 1 cut(s) 606
KroNI GCCGGC 1 cut(s) 608
Ksp22I TGATCA 2 cut(s) 351, 391
Kzo9I GATC 7 cut(s) 351, 385, 391, 548, 691, 838, 1060
LguI GCTCTTC 2 cut(s) 426, 596
LmnI GCTCC 4 cut(s) 14, 674, 733, 1030
Lsp1109I GCAGC 2 cut(s) 481, 665
LweI GCATC 2 cut(s) 633, 797
MaeI CTAG 3 cut(s) 231, 621, 819
MaeIII GTNAC 4 cut(s) 263, 613, 754, 1034
MalI GATC 7 cut(s) 353, 387, 393, 550, 693, 840, 1062
MboI GATC 7 cut(s) 351, 385, 391, 548, 691, 838, 1060
MboII GAAGA 8 cut(s) 93, 273, 443, 613, 644, 647, 701, 879
MflI RGATCY 1 cut(s) 548
MhlI GDGCHC 4 cut(s) 657, 671, 898, 915
MluCI AATT 9 cut(s) 97, 126, 214, 364, 479, 519, 535, 724, 987
MlyI GAGTC 1 cut(s) 784
MmeI TCCRAC 1 cut(s) 803
MnlI CCTC 8 cut(s) 19, 49, 58, 61, 127, 143, 202, 508
MroNI GCCGGC 1 cut(s) 606
MroXI GAANNNNTTC 2 cut(s) 100, 567
MseI TTAA 3 cut(s) 522, 990, 998
MslI CAYNNNNRTG 2 cut(s) 320, 717
MspA1I CMGCKG 1 cut(s) 48
MspI CCGG 3 cut(s) 449, 607, 651
MspR9I CCNGG 4 cut(s) 147, 449, 652, 1052
MvaI CCWGG 2 cut(s) 147, 1052
MwoI GCNNNNNNNGC 1 cut(s) 675
NaeI GCCGGC 1 cut(s) 608
NciI CCSGG 2 cut(s) 449, 652
NcoI CCATGG 1 cut(s) 510
NdeII GATC 7 cut(s) 351, 385, 391, 548, 691, 838, 1060
NgoMIV GCCGGC 1 cut(s) 606
NlaIII CATG 6 cut(s) 82, 124, 295, 392, 514, 722
NmuCI GTSAC 1 cut(s) 613
PagI TCATGA 1 cut(s) 388
PaqCI CACCTGC 1 cut(s) 601
PciSI GCTCTTC 2 cut(s) 426, 596
PdiI GCCGGC 1 cut(s) 608
PdmI GAANNNNTTC 2 cut(s) 100, 567
PfeI GAWTC 2 cut(s) 194, 278
PkrI GCNGC 2 cut(s) 496, 680
PleI GAGTC 1 cut(s) 783
PpsI GAGTC 1 cut(s) 783
Psp124BI GAGCTC 1 cut(s) 671
Psp6I CCWGG 2 cut(s) 145, 1050
PspFI CCCAGC 1 cut(s) 341
PspGI CCWGG 2 cut(s) 145, 1050
PspPI GGNCC 3 cut(s) 36, 904, 1048
PstI CTGCAG 1 cut(s) 903
PstNI CAGNNNCTG 3 cut(s) 678, 736, 836
PsuI RGATCY 1 cut(s) 548
RsaI GTAC 3 cut(s) 56, 229, 643
RsaNI GTAC 3 cut(s) 55, 228, 642
RseI CAYNNNNRTG 2 cut(s) 320, 717
SacI GAGCTC 1 cut(s) 671
SapI GCTCTTC 2 cut(s) 426, 596
SaqAI TTAA 3 cut(s) 522, 990, 998
SatI GCNGC 2 cut(s) 495, 679
Sau3AI GATC 7 cut(s) 351, 385, 391, 548, 691, 838, 1060
Sau96I GGNCC 3 cut(s) 36, 904, 1048
SbfI CCTGCAGG 1 cut(s) 903
SchI GAGTC 1 cut(s) 784
ScrFI CCNGG 4 cut(s) 147, 449, 652, 1052
SdaI CCTGCAGG 1 cut(s) 903
SduI GDGCHC 4 cut(s) 657, 671, 898, 915
SfaNI GCATC 2 cut(s) 633, 797
SfcI CTRYAG 1 cut(s) 899
SinI GGWCC 2 cut(s) 36, 1048
SmiMI CAYNNNNRTG 2 cut(s) 320, 717
SmlI CTYRAG 3 cut(s) 772, 799, 947
SmoI CTYRAG 3 cut(s) 772, 799, 947
Sse8387I CCTGCAGG 1 cut(s) 903
Sse9I AATT 9 cut(s) 97, 126, 214, 364, 479, 519, 535, 724, 987
SsiI CCGC 3 cut(s) 48, 441, 648
SspMI CTAG 3 cut(s) 231, 621, 819
SstI GAGCTC 1 cut(s) 671
StyD4I CCNGG 4 cut(s) 145, 447, 650, 1050
StyI CCWWGG 1 cut(s) 510
TaaI ACNGT 2 cut(s) 641, 784
TasI AATT 9 cut(s) 97, 126, 214, 364, 479, 519, 535, 724, 987
TatI WGTACW 2 cut(s) 54, 641
TfiI GAWTC 2 cut(s) 194, 278
Tru1I TTAA 3 cut(s) 522, 990, 998
Tru9I TTAA 3 cut(s) 522, 990, 998
TscAI CASTG 3 cut(s) 64, 706, 841
TseFI GTSAC 1 cut(s) 613
TseI GCWGC 2 cut(s) 494, 678
Tsp45I GTSAC 1 cut(s) 613
TspGWI ACGGA 1 cut(s) 933
TspRI CASTG 3 cut(s) 64, 706, 841
VpaK11BI GGWCC 2 cut(s) 36, 1048
XapI RAATTY 6 cut(s) 97, 126, 364, 479, 535, 724
XcmI CCANNNNNNNNNTGG 1 cut(s) 138
XmnI GAANNNNTTC 2 cut(s) 100, 567
XspI CTAG 3 cut(s) 231, 621, 819
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.