MD15G1071200.v1.1

Transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0002575
Physical Location & Seq
Reverse (-)
4877721 .. 4879404
1684 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1071200.v1.1.491

Sequence Viewer

Length: 1080 bp
ATGGAAGCCGTTGGTGTTTTTGCTGATGGGGAATGGGAATCCTTGAGCAGGTTGTTTTCCTCCGAAGAGGTTGAGTTCACACCACATTTTCTTTCCCAGGGAAATTCATTCCCTTTCCAGCACAATGAGGGAATGGGATTTGAAACCCTAGAAACTTTTTACCCTAATATTCCTGGAGTTGAAACTTTTTCTCACCTTCCTTCTTCAGATAGTCCCAACTCTAATGTCTATCTTTCTTCTCAAGAAAGTTGCAACTATGGTAATAATAATCATGGCAATTTCACTGCCATGCTGAACCAGGAAAATTGCTACTTTAGAAATTCTTGCAATTTCCTAGTGAGTAATATTGATGTCTCTGAATCCATGGATGTTTATATGAATCATGATGAAAAGAGTTTCGCCTCCTTCGTCCCGGATTTTTCCAACATTGTGATGCATGGAAGTGAGTGCAACAAAGAGGATTCCAGCAGTGATAGCCAACCGGCTGTTGTTTCGGTTCCTGAAAAGGAATTGCGGCTCAAAAGGGTGCATGATGCGCCGGGAAAATCCAACAATGATGCATCCAACTGCAATCCGAAGAAGAAACCTCATATTTCGAAAGATGCAGAGAAAAGTAAGAAAAATAATGTAAGGCCAAAGAAGGGGCAAAAGGGTAATTCAAATGTGAAAGTCAAAGATGAAGAAGAGAGGCTGGAGAGCACAAGTTCTTGCACCTCTGAGGATGATAATGGTTCTCTGGAGATCAATGGAGCAGAAACTTCAGACCCCAAATCTTCATCTGCCCTAAACTTGAATGGGAAGACCAGAGCCAATAGAGGGTCTGCAACTGATCCCCAGAGCCTCTATGCTAGGAAAAGAAGAGAGAGAATCAACGAGAGACTAAGAACCCTACAGCATCTTGTCCCTAATGGAACAAAGGTTGACATCAGCACAATGCTTGAAGAGGCAGTTCACTATGTCAACTTTTTGCAGCTCCAAATCAAGCTTTTAGGCTCAGATGATATGTGGATGTATGCTCCCATTGTTTATAATGGAATTGATATTGGTCTTGACCTGCAGAAGCTCTTTCCACTTCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000902 GO:0000904 GO:0000976 GO:0000981 GO:0001046 GO:0001047 GO:0001067 GO:0001228 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009888 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010015 GO:0010033 GO:0010053 GO:0010054 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016043 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0021700 GO:0022622 GO:0030154 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032501 GO:0032502 GO:0032774 GO:0032989 GO:0034641 GO:0034645 GO:0034654 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048364 GO:0048468 GO:0048469 GO:0048518 GO:0048522 GO:0048731 GO:0048764 GO:0048765 GO:0048766 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0060255 GO:0065007 GO:0071695 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0090558 GO:0090627 GO:0097159 GO:0097659 GO:0099402 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:1905392 GO:1990837 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HLH

Protein Analysis

360

Amino Acids

40.14

Weight (kDa)

5.23

Isoelectric Point (pI)

54.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 284 - 324 3.8e-08 Helix-loop-helix DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1029
Acc36I ACCTGC 2 cut(s) 39, 1062
AciI CCGC 1 cut(s) 514
AclWI GGATC 1 cut(s) 824
AcsI RAATTY 2 cut(s) 103, 319
AcuI CTGAAG 2 cut(s) 189, 744
AfiI CCNNNNNNNGG 3 cut(s) 48, 641, 816
AgsI TTSAA 5 cut(s) 143, 182, 660, 793, 941
AjnI CCWGG 3 cut(s) 96, 172, 297
AluBI AGCT 3 cut(s) 973, 985, 1063
AluI AGCT 3 cut(s) 973, 985, 1063
Alw21I GWGCWC 1 cut(s) 701
Alw26I GTCTC 2 cut(s) 358, 871
AlwI GGATC 1 cut(s) 824
AoxI GGCC 1 cut(s) 632
ApeKI GCWGC 1 cut(s) 970
ApoI RAATTY 2 cut(s) 103, 319
AspLEI GCGC 1 cut(s) 538
AsuC2I CCSGG 2 cut(s) 413, 540
AsuHPI GGTGA 1 cut(s) 185
AsuII TTCGAA 1 cut(s) 596
BbsI GAAGAC 1 cut(s) 806
Bbv12I GWGCWC 1 cut(s) 701
BbvI GCAGC 1 cut(s) 982
BccI CCATC 1 cut(s) 20
BciT130I CCWGG 3 cut(s) 98, 174, 299
BcnI CCSGG 2 cut(s) 413, 540
BcoDI GTCTC 2 cut(s) 358, 871
BfaI CTAG 3 cut(s) 149, 335, 849
BfmI CTRYAG 2 cut(s) 890, 1055
BfuAI ACCTGC 2 cut(s) 39, 1062
BisI GCNGC 2 cut(s) 515, 971
BlsI GCNGC 2 cut(s) 516, 972
Bme1390I CCNGG 5 cut(s) 98, 174, 299, 413, 540
BmiI GGNNCC 1 cut(s) 498
BmrFI CCNGG 5 cut(s) 98, 174, 299, 413, 540
BmsI GCATC 6 cut(s) 423, 523, 547, 569, 592, 904
BpiI GAAGAC 1 cut(s) 806
BpmI CTGGAG 3 cut(s) 195, 713, 758
Bpu14I TTCGAA 1 cut(s) 596
BpuEI CTTGAG 2 cut(s) 64, 225
BpuMI CCSGG 2 cut(s) 413, 540
BsaJI CCNNGG 3 cut(s) 96, 97, 363
Bsc4I CCNNNNNNNGG 3 cut(s) 48, 641, 816
Bse118I RCCGGY 1 cut(s) 481
BseBI CCWGG 3 cut(s) 98, 174, 299
BseDI CCNNGG 3 cut(s) 96, 97, 363
BseGI GGATG 4 cut(s) 373, 560, 727, 1014
BseLI CCNNNNNNNGG 3 cut(s) 48, 641, 816
BseMII CTCAG 2 cut(s) 708, 1008
BseXI GCAGC 1 cut(s) 982
BshFI GGCC 1 cut(s) 634
BsiHKAI GWGCWC 1 cut(s) 701
BsiSI CCGG 3 cut(s) 413, 482, 539
BslFI GGGAC 3 cut(s) 198, 395, 887
BslI CCNNNNNNNGG 3 cut(s) 48, 641, 816
BsmAI GTCTC 2 cut(s) 358, 871
BsmFI GGGAC 3 cut(s) 198, 395, 887
BsnI GGCC 1 cut(s) 634
Bsp119I TTCGAA 1 cut(s) 596
Bsp1286I GDGCHC 1 cut(s) 701
Bsp143I GATC 2 cut(s) 741, 829
Bsp19I CCATGG 1 cut(s) 363
BspACI CCGC 1 cut(s) 514
BspANI GGCC 1 cut(s) 634
BspCNI CTCAG 2 cut(s) 709, 1007
BspHI TCATGA 1 cut(s) 382
BspLI GGNNCC 1 cut(s) 498
BspMAI CTGCAG 1 cut(s) 1059
BspMI ACCTGC 2 cut(s) 39, 1062
BspPI GGATC 1 cut(s) 824
BspT104I TTCGAA 1 cut(s) 596
BsrFI RCCGGY 1 cut(s) 481
BssAI RCCGGY 1 cut(s) 481
BssECI CCNNGG 3 cut(s) 96, 97, 363
BssMI GATC 2 cut(s) 741, 829
BssT1I CCWWGG 1 cut(s) 363
Bst2UI CCWGG 3 cut(s) 98, 174, 299
Bst6I CTCTTC 4 cut(s) 60, 678, 853, 936
BstBI TTCGAA 1 cut(s) 596
BstDEI CTNAG 3 cut(s) 717, 881, 994
BstDSI CCRYGG 1 cut(s) 363
BstENI CCTNNNNNAGG 1 cut(s) 46
BstF5I GGATG 4 cut(s) 373, 560, 727, 1014
BstHHI GCGC 1 cut(s) 538
BstKTI GATC 2 cut(s) 744, 832
BstMAI GTCTC 2 cut(s) 358, 871
BstMBI GATC 2 cut(s) 741, 829
BstMWI GCNNNNNNNGC 2 cut(s) 474, 535
BstNI CCWGG 3 cut(s) 98, 174, 299
BstSCI CCNGG 5 cut(s) 96, 172, 297, 411, 538
BstSFI CTRYAG 2 cut(s) 890, 1055
BstV1I GCAGC 1 cut(s) 982
BstV2I GAAGAC 1 cut(s) 806
BsuRI GGCC 1 cut(s) 634
BtgI CCRYGG 1 cut(s) 363
BtsCI GGATG 4 cut(s) 373, 560, 727, 1014
BtsI GCAGTG 2 cut(s) 282, 475
BtsIMutI CAGTG 2 cut(s) 282, 475
BveI ACCTGC 2 cut(s) 39, 1062
CciI TCATGA 1 cut(s) 382
CfoI GCGC 1 cut(s) 538
Cfr10I RCCGGY 1 cut(s) 481
CviAII CATG 6 cut(s) 272, 289, 364, 383, 437, 530
DdeI CTNAG 3 cut(s) 717, 881, 994
DpnI GATC 2 cut(s) 743, 831
DpnII GATC 2 cut(s) 741, 829
Eam1104I CTCTTC 4 cut(s) 60, 678, 853, 936
EarI CTCTTC 4 cut(s) 60, 678, 853, 936
Eco130I CCWWGG 1 cut(s) 363
Eco57I CTGAAG 2 cut(s) 189, 744
EcoNI CCTNNNNNAGG 1 cut(s) 46
EcoRII CCWGG 3 cut(s) 96, 172, 297
EcoT14I CCWWGG 1 cut(s) 363
EcoT22I ATGCAT 2 cut(s) 438, 562
ErhI CCWWGG 1 cut(s) 363
FaeI CATG 6 cut(s) 275, 292, 367, 386, 440, 533
FaqI GGGAC 3 cut(s) 198, 395, 887
FatI CATG 6 cut(s) 271, 288, 363, 382, 436, 529
Fnu4HI GCNGC 2 cut(s) 515, 971
FokI GGATG 4 cut(s) 380, 547, 734, 1021
Fsp4HI GCNGC 2 cut(s) 515, 971
FspBI CTAG 3 cut(s) 149, 335, 849
GlaI GCGC 1 cut(s) 537
GluI GCNGC 2 cut(s) 515, 971
GsuI CTGGAG 3 cut(s) 195, 713, 758
HaeIII GGCC 1 cut(s) 634
HapII CCGG 3 cut(s) 413, 482, 539
HhaI GCGC 1 cut(s) 538
Hin1II CATG 6 cut(s) 275, 292, 367, 386, 440, 533
Hin6I GCGC 1 cut(s) 536
HinP1I GCGC 1 cut(s) 536
HincII GTYRAC 2 cut(s) 922, 961
HindII GTYRAC 2 cut(s) 922, 961
HindIII AAGCTT 1 cut(s) 983
HinfI GANTC 5 cut(s) 38, 359, 379, 461, 867
HpaII CCGG 3 cut(s) 413, 482, 539
HphI GGTGA 1 cut(s) 185
Hpy166II GTNNAC 4 cut(s) 78, 922, 952, 961
Hpy188I TCNGA 7 cut(s) 64, 208, 358, 576, 718, 763, 997
Hpy188III TCNNGA 5 cut(s) 242, 383, 500, 737, 1049
Hpy8I GTNNAC 4 cut(s) 78, 922, 952, 961
HpyAV CCTTC 4 cut(s) 206, 210, 415, 634
HpyF10VI GCNNNNNNNGC 2 cut(s) 474, 535
HpyF3I CTNAG 3 cut(s) 717, 881, 994
Hsp92II CATG 6 cut(s) 275, 292, 367, 386, 440, 533
HspAI GCGC 1 cut(s) 536
Kzo9I GATC 2 cut(s) 741, 829
LmnI GCTCC 3 cut(s) 749, 978, 1021
Lsp1109I GCAGC 1 cut(s) 982
LweI GCATC 6 cut(s) 423, 523, 547, 569, 592, 904
MaeI CTAG 3 cut(s) 149, 335, 849
MalI GATC 2 cut(s) 743, 831
MboI GATC 2 cut(s) 741, 829
MhlI GDGCHC 1 cut(s) 701
MluCI AATT 8 cut(s) 103, 277, 304, 319, 328, 509, 655, 1035
MmeI TCCRAC 3 cut(s) 447, 573, 588
Mph1103I ATGCAT 2 cut(s) 438, 562
MslI CAYNNNNRTG 4 cut(s) 287, 431, 441, 1075
MspI CCGG 3 cut(s) 413, 482, 539
MspR9I CCNGG 5 cut(s) 98, 174, 299, 413, 540
MvaI CCWGG 3 cut(s) 98, 174, 299
MwoI GCNNNNNNNGC 2 cut(s) 474, 535
NciI CCSGG 2 cut(s) 413, 540
NcoI CCATGG 1 cut(s) 363
NdeII GATC 2 cut(s) 741, 829
NlaIII CATG 6 cut(s) 275, 292, 367, 386, 440, 533
NlaIV GGNNCC 1 cut(s) 498
NsiI ATGCAT 2 cut(s) 438, 562
NspV TTCGAA 1 cut(s) 596
PagI TCATGA 1 cut(s) 382
PasI CCCWGGG 1 cut(s) 97
PcsI WCGNNNNNNNCGW 1 cut(s) 405
PfeI GAWTC 5 cut(s) 38, 359, 379, 461, 867
PfoI TCCNGGA 2 cut(s) 172, 411
PkrI GCNGC 2 cut(s) 516, 972
PsiI TTATAA 1 cut(s) 1029
Psp6I CCWGG 3 cut(s) 96, 172, 297
PspGI CCWGG 3 cut(s) 96, 172, 297
PspN4I GGNNCC 1 cut(s) 498
PstI CTGCAG 1 cut(s) 1059
RseI CAYNNNNRTG 4 cut(s) 287, 431, 441, 1075
SatI GCNGC 2 cut(s) 515, 971
Sau3AI GATC 2 cut(s) 741, 829
ScrFI CCNGG 5 cut(s) 98, 174, 299, 413, 540
SduI GDGCHC 1 cut(s) 701
SfaNI GCATC 6 cut(s) 423, 523, 547, 569, 592, 904
SfcI CTRYAG 2 cut(s) 890, 1055
SfuI TTCGAA 1 cut(s) 596
SmiMI CAYNNNNRTG 4 cut(s) 287, 431, 441, 1075
SmlI CTYRAG 2 cut(s) 43, 240
SmoI CTYRAG 2 cut(s) 43, 240
Sse9I AATT 8 cut(s) 103, 277, 304, 319, 328, 509, 655, 1035
SsiI CCGC 1 cut(s) 514
SspI AATATT 2 cut(s) 169, 346
SspMI CTAG 3 cut(s) 149, 335, 849
StyD4I CCNGG 5 cut(s) 96, 172, 297, 411, 538
StyI CCWWGG 1 cut(s) 363
TaqI TCGA 1 cut(s) 596
TasI AATT 8 cut(s) 103, 277, 304, 319, 328, 509, 655, 1035
TauI GCSGC 1 cut(s) 517
TfiI GAWTC 5 cut(s) 38, 359, 379, 461, 867
TscAI CASTG 2 cut(s) 289, 475
TseI GCWGC 1 cut(s) 970
TspDTI ATGAA 5 cut(s) 96, 392, 402, 693, 765
TspRI CASTG 2 cut(s) 289, 475
XagI CCTNNNNNAGG 1 cut(s) 46
XapI RAATTY 2 cut(s) 103, 319
XspI CTAG 3 cut(s) 149, 335, 849
Zsp2I ATGCAT 2 cut(s) 438, 562
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.