FvH4_2g13260

Protein of unknown function (DUF502)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0002755
Physical Location & Seq
Forward (+)
11583810 .. 11590732
6923 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g13260.t1

Sequence Viewer

Length: 747 bp
ATGGCGGAATCTAAAGAGTCCACGTCGTCGAGTCCCCTCACTCAACCCGATCCTGAAGATTTTCCCAAGTCTCCGCCCAGCTCCCCCAACTCCTCCACTCGCAAGGCTTGCTATGCAGTGCTCCAAAGTTGGGTCTCAAAGAAGTTCATGACTGGATGTATGGTTCTCTTTCCTGTTGCTGTGACATTCTTCATAACCTGGTGGTTTATACAGTTCGTGGATGGTTTCTTCAGTCCATTATATGCCAGGCTTGGTATTAATATATTTGGCCTCGGGTTTATTACATCCCTAATTTTTATGTTCTTAGTCGGAGTATTTGCTTCGTCATGGATGGGTGCAACTGTTTTCTCGGTGGGAGAATGGTTCATCAAACGAATGCCCTTTATGAAGCACATTTACTCAGCCTCCAAACAAATTAGTGCTGCAATTTCTCCAGACCAAAATACCACTGCTTTTAAAGAAGTTGCAATCATCCGTCATCCTAGGATTGGTGAATACGCGTTCGGTTTTATCACATCATCTGTAATCCTTCAGCGTGATGATGGAGATGAGGAGTTGTGCAGTGTTTATGTCCCAACGAACCATCTATACATTGGAGATATCTTTTTGGTCAATTCCAGAGAGATCATACGACCAAATTTGTCAATTCGAGAAGGCATAGAGATCATAATATCAGTTGGTATGACAATGCCACAGGTGATTTCTCCAATTGAAAGGGTTCCTCTTAAGAGGATGGTATCTCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF502

Protein Analysis

249

Amino Acids

27.84

Weight (kDa)

7.71

Isoelectric Point (pI)

58.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF502 PF04367 95 - 195 3.2e-26 Protein of unknown function (DUF502)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 500
AciI CCGC 2 cut(s) 5, 74
AclWI GGATC 1 cut(s) 44
AcsI RAATTY 1 cut(s) 637
AcuI CTGAAG 3 cut(s) 75, 214, 515
AfiI CCNNNNNNNGG 2 cut(s) 130, 488
AflII CTTAAG 1 cut(s) 725
AflIII ACRYGT 1 cut(s) 498
AgsI TTSAA 1 cut(s) 713
AjiI CACGTC 1 cut(s) 24
AjnI CCWGG 2 cut(s) 197, 245
AloI GAACNNNNNNTCC 2 cut(s) 147, 179
AluBI AGCT 1 cut(s) 81
AluI AGCT 1 cut(s) 81
Alw21I GWGCWC 1 cut(s) 123
Alw26I GTCTC 2 cut(s) 75, 139
AlwI GGATC 1 cut(s) 44
Ama87I CYCGRG 1 cut(s) 272
AoxI GGCC 1 cut(s) 268
ApeKI GCWGC 1 cut(s) 422
ApoI RAATTY 1 cut(s) 637
AseI ATTAAT 1 cut(s) 258
Asp700I GAANNNNTTC 2 cut(s) 60, 717
AspA2I CCTAGG 1 cut(s) 482
AsuHPI GGTGA 2 cut(s) 503, 709
AvaI CYCGRG 1 cut(s) 272
AvrII CCTAGG 1 cut(s) 482
BarI GAAGNNNNNNTAC 2 cut(s) 380, 412
Bbv12I GWGCWC 1 cut(s) 123
BbvI GCAGC 1 cut(s) 409
BccI CCATC 5 cut(s) 215, 325, 536, 591, 727
BciT130I CCWGG 2 cut(s) 199, 247
BcoDI GTCTC 2 cut(s) 75, 139
BfaI CTAG 1 cut(s) 483
BfrI CTTAAG 1 cut(s) 725
BisI GCNGC 1 cut(s) 423
BlnI CCTAGG 1 cut(s) 482
BlsI GCNGC 1 cut(s) 424
Bme1390I CCNGG 2 cut(s) 199, 247
BmeT110I CYCGRG 1 cut(s) 272
BmgBI CACGTC 1 cut(s) 24
BmiI GGNNCC 1 cut(s) 720
BmrFI CCNGG 2 cut(s) 199, 247
BpmI CTGGAG 1 cut(s) 417
BsaI GGTCTC 1 cut(s) 139
BsaJI CCNNGG 2 cut(s) 271, 482
BsaXI ACNNNNNCTCC 2 cut(s) 389, 419
Bsc4I CCNNNNNNNGG 2 cut(s) 130, 488
Bse1I ACTGG 1 cut(s) 157
BseBI CCWGG 2 cut(s) 199, 247
BseDI CCNNGG 2 cut(s) 271, 482
BseGI GGATG 7 cut(s) 161, 226, 284, 336, 471, 478, 738
BseLI CCNNNNNNNGG 2 cut(s) 130, 488
BseMII CTCAG 1 cut(s) 414
BseNI ACTGG 1 cut(s) 157
BseRI GAGGAG 2 cut(s) 82, 566
BseXI GCAGC 1 cut(s) 409
BseYI CCCAGC 1 cut(s) 77
BsgI GTGCAG 1 cut(s) 580
Bsh1236I CGCG 1 cut(s) 500
BshFI GGCC 1 cut(s) 270
BsiHKAI GWGCWC 1 cut(s) 123
BsiHKCI CYCGRG 1 cut(s) 272
BslFI GGGAC 2 cut(s) 18, 557
BslI CCNNNNNNNGG 2 cut(s) 130, 488
BsmAI GTCTC 2 cut(s) 75, 139
BsmFI GGGAC 2 cut(s) 18, 557
BsmI GAATGC 1 cut(s) 381
BsnI GGCC 1 cut(s) 270
Bso31I GGTCTC 1 cut(s) 139
BsoBI CYCGRG 1 cut(s) 272
Bsp1286I GDGCHC 1 cut(s) 123
Bsp143I GATC 3 cut(s) 49, 624, 663
BspACI CCGC 2 cut(s) 5, 74
BspANI GGCC 1 cut(s) 270
BspCNI CTCAG 1 cut(s) 413
BspFNI CGCG 1 cut(s) 500
BspHI TCATGA 1 cut(s) 147
BspLI GGNNCC 1 cut(s) 720
BspPI GGATC 1 cut(s) 44
BspTI CTTAAG 1 cut(s) 725
BspTNI GGTCTC 1 cut(s) 139
BsrI ACTGG 1 cut(s) 157
BssECI CCNNGG 2 cut(s) 271, 482
BssMI GATC 3 cut(s) 49, 624, 663
BssT1I CCWWGG 1 cut(s) 482
Bst2UI CCWGG 2 cut(s) 199, 247
Bst4CI ACNGT 2 cut(s) 213, 343
BstAFI CTTAAG 1 cut(s) 725
BstAPI GCANNNNNTGC 1 cut(s) 108
BstC8I GCNNGC 1 cut(s) 109
BstDEI CTNAG 2 cut(s) 304, 400
BstF5I GGATG 7 cut(s) 161, 226, 284, 336, 471, 478, 738
BstFNI CGCG 1 cut(s) 500
BstKTI GATC 3 cut(s) 52, 627, 666
BstMAI GTCTC 2 cut(s) 75, 139
BstMBI GATC 3 cut(s) 49, 624, 663
BstMWI GCNNNNNNNGC 2 cut(s) 108, 113
BstNI CCWGG 2 cut(s) 199, 247
BstSCI CCNGG 2 cut(s) 197, 245
BstUI CGCG 1 cut(s) 500
BstV1I GCAGC 1 cut(s) 409
BsuRI GGCC 1 cut(s) 270
BtrI CACGTC 1 cut(s) 24
BtsCI GGATG 7 cut(s) 161, 226, 284, 336, 471, 478, 738
BtsI GCAGTG 3 cut(s) 123, 447, 568
BtsIMutI CAGTG 3 cut(s) 123, 447, 568
Cac8I GCNNGC 1 cut(s) 109
CciI TCATGA 1 cut(s) 147
CsiI ACCWGGT 1 cut(s) 197
CviAII CATG 2 cut(s) 148, 327
CviJI RGCY 5 cut(s) 81, 107, 250, 270, 404
CviKI_1 RGCY 5 cut(s) 81, 107, 250, 270, 404
DdeI CTNAG 2 cut(s) 304, 400
DpnI GATC 3 cut(s) 51, 626, 665
DpnII GATC 3 cut(s) 49, 624, 663
DraI TTTAAA 1 cut(s) 457
EciI GGCGGA 2 cut(s) 20, 63
Eco130I CCWWGG 1 cut(s) 482
Eco31I GGTCTC 1 cut(s) 139
Eco32I GATATC 1 cut(s) 601
Eco57I CTGAAG 3 cut(s) 75, 214, 515
Eco88I CYCGRG 1 cut(s) 272
EcoRII CCWGG 2 cut(s) 197, 245
EcoRV GATATC 1 cut(s) 601
EcoT14I CCWWGG 1 cut(s) 482
ErhI CCWWGG 1 cut(s) 482
FaeI CATG 2 cut(s) 151, 330
FaqI GGGAC 2 cut(s) 18, 557
FatI CATG 2 cut(s) 147, 326
Fnu4HI GCNGC 1 cut(s) 423
FokI GGATG 6 cut(s) 168, 233, 271, 343, 458, 465
Fsp4HI GCNGC 1 cut(s) 423
FspBI CTAG 1 cut(s) 483
GluI GCNGC 1 cut(s) 423
GsaI CCCAGC 1 cut(s) 81
GsuI CTGGAG 1 cut(s) 417
HaeIII GGCC 1 cut(s) 270
Hin1II CATG 2 cut(s) 151, 330
HinfI GANTC 3 cut(s) 8, 17, 31
HphI GGTGA 2 cut(s) 503, 709
Hpy166II GTNNAC 1 cut(s) 21
Hpy188I TCNGA 1 cut(s) 311
Hpy188III TCNNGA 5 cut(s) 53, 148, 434, 618, 650
Hpy8I GTNNAC 1 cut(s) 21
Hpy99I CGWCG 2 cut(s) 28, 31
HpyAV CCTTC 2 cut(s) 539, 647
HpyCH4III ACNGT 2 cut(s) 213, 343
HpyCH4IV ACGT 1 cut(s) 23
HpyCH4V TGCA 5 cut(s) 116, 338, 425, 467, 561
HpyF10VI GCNNNNNNNGC 2 cut(s) 108, 113
HpyF3I CTNAG 2 cut(s) 304, 400
HpySE526I ACGT 1 cut(s) 23
Hsp92II CATG 2 cut(s) 151, 330
Kzo9I GATC 3 cut(s) 49, 624, 663
LmnI GCTCC 2 cut(s) 86, 126
Lsp1109I GCAGC 1 cut(s) 409
MabI ACCWGGT 1 cut(s) 197
MaeI CTAG 1 cut(s) 483
MaeII ACGT 1 cut(s) 23
MaeIII GTNAC 1 cut(s) 181
MalI GATC 3 cut(s) 51, 626, 665
MboI GATC 3 cut(s) 49, 624, 663
MboII GAAGA 3 cut(s) 68, 181, 220
MfeI CAATTG 1 cut(s) 708
MhlI GDGCHC 1 cut(s) 123
MluCI AATT 7 cut(s) 291, 414, 426, 613, 637, 645, 708
MluI ACGCGT 1 cut(s) 498
MlyI GAGTC 2 cut(s) 26, 40
MmeI TCCRAC 1 cut(s) 289
MnlI CCTC 7 cut(s) 47, 103, 281, 415, 544, 723, 732
MroXI GAANNNNTTC 2 cut(s) 60, 717
MseI TTAA 3 cut(s) 258, 456, 726
MspCI CTTAAG 1 cut(s) 725
MspR9I CCNGG 2 cut(s) 199, 247
MunI CAATTG 1 cut(s) 708
Mva1269I GAATGC 1 cut(s) 381
MvaI CCWGG 2 cut(s) 199, 247
MvnI CGCG 1 cut(s) 500
MwoI GCNNNNNNNGC 2 cut(s) 108, 113
NdeII GATC 3 cut(s) 49, 624, 663
NlaIII CATG 2 cut(s) 151, 330
NlaIV GGNNCC 1 cut(s) 720
NmuCI GTSAC 1 cut(s) 181
PagI TCATGA 1 cut(s) 147
PctI GAATGC 1 cut(s) 381
PdmI GAANNNNTTC 2 cut(s) 60, 717
PfeI GAWTC 1 cut(s) 8
PkrI GCNGC 1 cut(s) 424
PleI GAGTC 2 cut(s) 25, 39
PpsI GAGTC 2 cut(s) 25, 39
PshBI ATTAAT 1 cut(s) 258
Psp6I CCWGG 2 cut(s) 197, 245
PspFI CCCAGC 1 cut(s) 77
PspGI CCWGG 2 cut(s) 197, 245
PspN4I GGNNCC 1 cut(s) 720
PsrI GAACNNNNNNTAC 2 cut(s) 572, 604
SaqAI TTAA 3 cut(s) 258, 456, 726
SatI GCNGC 1 cut(s) 423
Sau3AI GATC 3 cut(s) 49, 624, 663
SchI GAGTC 2 cut(s) 26, 40
ScrFI CCNGG 2 cut(s) 199, 247
SduI GDGCHC 1 cut(s) 123
SetI ASST 4 cut(s) 26, 83, 200, 699
SexAI ACCWGGT 1 cut(s) 197
SmlI CTYRAG 1 cut(s) 725
SmoI CTYRAG 1 cut(s) 725
Sse9I AATT 7 cut(s) 291, 414, 426, 613, 637, 645, 708
SsiI CCGC 2 cut(s) 5, 74
SspMI CTAG 1 cut(s) 483
StyD4I CCNGG 2 cut(s) 197, 245
StyI CCWWGG 1 cut(s) 482
TaaI ACNGT 2 cut(s) 213, 343
TaiI ACGT 1 cut(s) 26
TaqI TCGA 2 cut(s) 29, 649
TasI AATT 7 cut(s) 291, 414, 426, 613, 637, 645, 708
TfiI GAWTC 1 cut(s) 8
Tru1I TTAA 3 cut(s) 258, 456, 726
Tru9I TTAA 3 cut(s) 258, 456, 726
TscAI CASTG 3 cut(s) 123, 454, 568
TseFI GTSAC 1 cut(s) 181
TseI GCWGC 1 cut(s) 422
Tsp45I GTSAC 1 cut(s) 181
TspDTI ATGAA 4 cut(s) 136, 181, 355, 401
TspGWI ACGGA 1 cut(s) 464
TspRI CASTG 3 cut(s) 123, 454, 568
Vha464I CTTAAG 1 cut(s) 725
VspI ATTAAT 1 cut(s) 258
XapI RAATTY 1 cut(s) 637
XcmI CCANNNNNNNNNTGG 1 cut(s) 590
XmaJI CCTAGG 1 cut(s) 482
XmnI GAANNNNTTC 2 cut(s) 60, 717
XspI CTAG 1 cut(s) 483
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.