MD10G1030700.v1.1

Protein of unknown function (DUF502)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0002755
Physical Location & Seq
Reverse (-)
4021909 .. 4027419
5511 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1030700.v1.1.491

Sequence Viewer

Length: 768 bp
ATGGCGCAAGCTAAAGAGTCCACGTCGTCGGGTCCGCTGACCCAACCCGACTTTGAAGATGTTTCCAGGTCTCCGCCAAGCTCCCCCAACTCCTCCACTCGCAAGGCCTGCTATGCAGTGCTTCAGAGTTGGGTCTCCAAGAAATTCATGACTGGATGTATGGTTCTCTTTCCTGTCACTGTTACGTTCTTCATCACTTGGTGGTTTGTTCAGTTTGTAGATGGTTTTTCCAGCCCAATATATGCCAGGCTTGGGGTTAGCATATTTGGCCTAGGATTTGTTACATCCCTACTTTTTATATTCTTGATTGGTATTTTTGCTTCATCATGGATGGGTGAGACAGTTTTCTGGATTGGAGAATGGTTCATAAAACGATTGCCCTTTATGAAGCACATATACTCGGCCTCTAAACAGATTAGTTCTGCGATCTCTCCAGATCAAAAGACCACTGCTTTTAAAGAGGTTGCAATTATCCGTCATCCTCGACTTGGTGAATATGCATTTGGTTTTATAACATCATCTGTAACCCTTCAGAGGGATGATGGAGACGAGGAGTTGTGTAGTGTTTATGTCCCAACAAACCATCTGTACATTGGCGATATTTTTCTTGTCAAATCTGAAGAGATCATACGACCAAATTTGTCAGTTCGGGAAGGCATAGAGATCATAATCTCAGTTGGTATGACGATGCCACAAGTGATTTCCCCAATTGAAAGGTTTCCTCATCAGAGTGACAGGATTCCACCGAACAGAATGGCATCCCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF502

Protein Analysis

256

Amino Acids

28.69

Weight (kDa)

6.91

Isoelectric Point (pI)

55.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF502 PF04367 95 - 195 6e-27 Protein of unknown function (DUF502)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 512
AciI CCGC 2 cut(s) 35, 74
AcsI RAATTY 2 cut(s) 143, 637
AcuI CTGAAG 3 cut(s) 107, 515, 639
AdeI CACNNNGTG 1 cut(s) 201
AfaI GTAC 1 cut(s) 590
AfiI CCNNNNNNNGG 4 cut(s) 252, 488, 534, 535
AgsI TTSAA 2 cut(s) 56, 713
AjiI CACGTC 1 cut(s) 24
AjnI CCWGG 2 cut(s) 65, 245
AjuI GAANNNNNNNTTGG 2 cut(s) 486, 518
AloI GAACNNNNNNTCC 2 cut(s) 147, 179
AluBI AGCT 2 cut(s) 11, 81
AluI AGCT 2 cut(s) 11, 81
Alw26I GTCTC 4 cut(s) 75, 139, 332, 540
AoxI GGCC 3 cut(s) 105, 268, 402
ApoI RAATTY 2 cut(s) 143, 637
Asp700I GAANNNNTTC 1 cut(s) 717
AspA2I CCTAGG 1 cut(s) 271
AspLEI GCGC 1 cut(s) 7
AspS9I GGNCC 1 cut(s) 32
AsuHPI GGTGA 2 cut(s) 347, 503
AvaII GGWCC 1 cut(s) 32
AvrII CCTAGG 1 cut(s) 271
BarI GAAGNNNNNNTAC 6 cut(s) 304, 336, 380, 412, 612, 644
BccI CCATC 4 cut(s) 215, 325, 536, 591
BciT130I CCWGG 2 cut(s) 67, 247
BcoDI GTCTC 4 cut(s) 75, 139, 332, 540
BfaI CTAG 1 cut(s) 272
BlnI CCTAGG 1 cut(s) 271
Bme1390I CCNGG 2 cut(s) 67, 247
Bme18I GGWCC 1 cut(s) 32
BmgBI CACGTC 1 cut(s) 24
BmgT120I GGNCC 1 cut(s) 32
BmiI GGNNCC 1 cut(s) 33
BmrFI CCNGG 2 cut(s) 67, 247
BmsI GCATC 1 cut(s) 678
BpmI CTGGAG 1 cut(s) 417
BsaBI GATNNNNATC 1 cut(s) 668
BsaI GGTCTC 2 cut(s) 75, 139
BsaJI CCNNGG 1 cut(s) 271
Bsc4I CCNNNNNNNGG 4 cut(s) 252, 488, 534, 535
Bse1I ACTGG 1 cut(s) 157
Bse8I GATNNNNATC 1 cut(s) 668
BseBI CCWGG 2 cut(s) 67, 247
BseDI CCNNGG 1 cut(s) 271
BseGI GGATG 6 cut(s) 161, 284, 336, 478, 544, 758
BseJI GATNNNNATC 1 cut(s) 668
BseLI CCNNNNNNNGG 4 cut(s) 252, 488, 534, 535
BseMII CTCAG 1 cut(s) 687
BseNI ACTGG 1 cut(s) 157
BseRI GAGGAG 2 cut(s) 82, 566
BshFI GGCC 3 cut(s) 107, 270, 404
BslFI GGGAC 1 cut(s) 557
BslI CCNNNNNNNGG 4 cut(s) 252, 488, 534, 535
BsmAI GTCTC 4 cut(s) 75, 139, 332, 540
BsmBI CGTCTC 1 cut(s) 540
BsmFI GGGAC 1 cut(s) 557
BsnI GGCC 3 cut(s) 107, 270, 404
Bso31I GGTCTC 2 cut(s) 75, 139
Bsp1407I TGTACA 1 cut(s) 588
Bsp143I GATC 4 cut(s) 426, 436, 624, 663
BspACI CCGC 2 cut(s) 35, 74
BspANI GGCC 3 cut(s) 107, 270, 404
BspCNI CTCAG 1 cut(s) 686
BspHI TCATGA 1 cut(s) 147
BspLI GGNNCC 1 cut(s) 33
BspTNI GGTCTC 2 cut(s) 75, 139
BsrGI TGTACA 1 cut(s) 588
BsrI ACTGG 1 cut(s) 157
BssECI CCNNGG 1 cut(s) 271
BssMI GATC 4 cut(s) 426, 436, 624, 663
BssT1I CCWWGG 1 cut(s) 271
Bst2UI CCWGG 2 cut(s) 67, 247
Bst4CI ACNGT 2 cut(s) 181, 343
Bst6I CTCTTC 1 cut(s) 615
BstAPI GCANNNNNTGC 1 cut(s) 108
BstAUI TGTACA 1 cut(s) 588
BstC8I GCNNGC 2 cut(s) 9, 109
BstDEI CTNAG 1 cut(s) 673
BstF5I GGATG 6 cut(s) 161, 284, 336, 478, 544, 758
BstHHI GCGC 1 cut(s) 7
BstKTI GATC 4 cut(s) 429, 439, 627, 666
BstMAI GTCTC 4 cut(s) 75, 139, 332, 540
BstMBI GATC 4 cut(s) 426, 436, 624, 663
BstMWI GCNNNNNNNGC 3 cut(s) 108, 113, 267
BstNI CCWGG 2 cut(s) 67, 247
BstSCI CCNGG 2 cut(s) 65, 245
BsuRI GGCC 3 cut(s) 107, 270, 404
BtrI CACGTC 1 cut(s) 24
BtsCI GGATG 6 cut(s) 161, 284, 336, 478, 544, 758
BtsI GCAGTG 2 cut(s) 123, 447
BtsIMutI CAGTG 3 cut(s) 123, 177, 447
Cac8I GCNNGC 2 cut(s) 9, 109
CciI TCATGA 1 cut(s) 147
CfoI GCGC 1 cut(s) 7
Cfr13I GGNCC 1 cut(s) 32
Csp6I GTAC 1 cut(s) 589
CviAII CATG 2 cut(s) 148, 327
CviJI RGCY 7 cut(s) 11, 81, 107, 234, 250, 270, 404
CviKI_1 RGCY 7 cut(s) 11, 81, 107, 234, 250, 270, 404
CviQI GTAC 1 cut(s) 589
DdeI CTNAG 1 cut(s) 673
DpnI GATC 4 cut(s) 428, 438, 626, 665
DpnII GATC 4 cut(s) 426, 436, 624, 663
DraI TTTAAA 1 cut(s) 457
DraIII CACNNNGTG 1 cut(s) 201
Eam1104I CTCTTC 1 cut(s) 615
EarI CTCTTC 1 cut(s) 615
EciI GGCGGA 1 cut(s) 63
Eco130I CCWWGG 1 cut(s) 271
Eco147I AGGCCT 1 cut(s) 107
Eco31I GGTCTC 2 cut(s) 75, 139
Eco47I GGWCC 1 cut(s) 32
Eco57I CTGAAG 3 cut(s) 107, 515, 639
EcoRII CCWGG 2 cut(s) 65, 245
EcoT14I CCWWGG 1 cut(s) 271
EcoT22I ATGCAT 1 cut(s) 502
ErhI CCWWGG 1 cut(s) 271
Esp3I CGTCTC 1 cut(s) 540
FaeI CATG 2 cut(s) 151, 330
FaqI GGGAC 1 cut(s) 557
FatI CATG 2 cut(s) 147, 326
FokI GGATG 6 cut(s) 168, 271, 343, 465, 551, 745
FspBI CTAG 1 cut(s) 272
GlaI GCGC 1 cut(s) 6
GsuI CTGGAG 1 cut(s) 417
HaeIII GGCC 3 cut(s) 107, 270, 404
HhaI GCGC 1 cut(s) 7
Hin1II CATG 2 cut(s) 151, 330
Hin6I GCGC 1 cut(s) 5
HinP1I GCGC 1 cut(s) 5
HinfI GANTC 2 cut(s) 17, 739
HphI GGTGA 2 cut(s) 347, 503
Hpy166II GTNNAC 1 cut(s) 21
Hpy188I TCNGA 4 cut(s) 126, 534, 619, 729
Hpy188III TCNNGA 5 cut(s) 148, 304, 349, 434, 650
Hpy8I GTNNAC 1 cut(s) 21
Hpy99I CGWCG 2 cut(s) 28, 31
HpyAV CCTTC 2 cut(s) 539, 647
HpyCH4III ACNGT 2 cut(s) 181, 343
HpyCH4IV ACGT 2 cut(s) 23, 185
HpyCH4V TGCA 3 cut(s) 116, 467, 500
HpyF10VI GCNNNNNNNGC 3 cut(s) 108, 113, 267
HpyF3I CTNAG 1 cut(s) 673
HpySE526I ACGT 2 cut(s) 23, 185
Hsp92II CATG 2 cut(s) 151, 330
HspAI GCGC 1 cut(s) 5
Kzo9I GATC 4 cut(s) 426, 436, 624, 663
LmnI GCTCC 1 cut(s) 86
LweI GCATC 1 cut(s) 678
MaeI CTAG 1 cut(s) 272
MaeII ACGT 2 cut(s) 23, 185
MaeIII GTNAC 5 cut(s) 175, 181, 280, 523, 731
MalI GATC 4 cut(s) 428, 438, 626, 665
MboI GATC 4 cut(s) 426, 436, 624, 663
MboII GAAGA 3 cut(s) 68, 181, 632
MfeI CAATTG 1 cut(s) 708
MluCI AATT 4 cut(s) 143, 468, 637, 708
MlyI GAGTC 1 cut(s) 26
MnlI CCTC 7 cut(s) 103, 415, 454, 492, 528, 544, 732
Mph1103I ATGCAT 1 cut(s) 502
MroXI GAANNNNTTC 1 cut(s) 717
MseI TTAA 2 cut(s) 456, 766
MslI CAYNNNNRTG 1 cut(s) 729
MspA1I CMGCKG 1 cut(s) 37
MspR9I CCNGG 2 cut(s) 67, 247
MunI CAATTG 1 cut(s) 708
MvaI CCWGG 2 cut(s) 67, 247
MwoI GCNNNNNNNGC 3 cut(s) 108, 113, 267
NdeII GATC 4 cut(s) 426, 436, 624, 663
NlaIII CATG 2 cut(s) 151, 330
NlaIV GGNNCC 1 cut(s) 33
NmeAIII GCCGAG 1 cut(s) 380
NmuCI GTSAC 2 cut(s) 175, 731
NsiI ATGCAT 1 cut(s) 502
PagI TCATGA 1 cut(s) 147
PceI AGGCCT 1 cut(s) 107
PdmI GAANNNNTTC 1 cut(s) 717
PfeI GAWTC 1 cut(s) 739
PleI GAGTC 1 cut(s) 25
PpsI GAGTC 1 cut(s) 25
PsiI TTATAA 1 cut(s) 512
Psp6I CCWGG 2 cut(s) 65, 245
PspGI CCWGG 2 cut(s) 65, 245
PspN4I GGNNCC 1 cut(s) 33
PspPI GGNCC 1 cut(s) 32
RsaI GTAC 1 cut(s) 590
RsaNI GTAC 1 cut(s) 589
RseI CAYNNNNRTG 1 cut(s) 729
SaqAI TTAA 2 cut(s) 456, 766
Sau3AI GATC 4 cut(s) 426, 436, 624, 663
Sau96I GGNCC 1 cut(s) 32
SchI GAGTC 1 cut(s) 26
ScrFI CCNGG 2 cut(s) 67, 247
SetI ASST 7 cut(s) 13, 26, 71, 83, 188, 465, 719
SfaNI GCATC 1 cut(s) 678
SinI GGWCC 1 cut(s) 32
SmiMI CAYNNNNRTG 1 cut(s) 729
Sse9I AATT 4 cut(s) 143, 468, 637, 708
SseBI AGGCCT 1 cut(s) 107
SsiI CCGC 2 cut(s) 35, 74
SspMI CTAG 1 cut(s) 272
StuI AGGCCT 1 cut(s) 107
StyD4I CCNGG 2 cut(s) 65, 245
StyI CCWWGG 1 cut(s) 271
TaaI ACNGT 2 cut(s) 181, 343
TaiI ACGT 2 cut(s) 26, 188
TaqI TCGA 1 cut(s) 484
TasI AATT 4 cut(s) 143, 468, 637, 708
TatI WGTACW 1 cut(s) 588
TfiI GAWTC 1 cut(s) 739
Tru1I TTAA 2 cut(s) 456, 766
Tru9I TTAA 2 cut(s) 456, 766
TscAI CASTG 3 cut(s) 123, 184, 454
TseFI GTSAC 2 cut(s) 175, 731
Tsp45I GTSAC 2 cut(s) 175, 731
TspDTI ATGAA 5 cut(s) 136, 181, 312, 355, 401
TspGWI ACGGA 1 cut(s) 464
TspRI CASTG 3 cut(s) 123, 184, 454
VpaK11BI GGWCC 1 cut(s) 32
XapI RAATTY 2 cut(s) 143, 637
XcmI CCANNNNNNNNNTGG 1 cut(s) 590
XmaJI CCTAGG 1 cut(s) 271
XmnI GAANNNNTTC 1 cut(s) 717
XspI CTAG 1 cut(s) 272
Zsp2I ATGCAT 1 cut(s) 502
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.