Rorug02G0226300

Protein of unknown function (DUF502)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0002755
Physical Location & Seq
Reverse (-)
22231106 .. 22236630
5525 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0226300.1

Sequence Viewer

Length: 1086 bp
ATGGAGGTCGCGGCATTCGTGCTGTGCTGGTGGGACGACATCAACGAGTCGACTCAGTGGCAAGACCGCATCTTCTACGCTCTCTGCACAGCCGAACCCCCTCGGCTCCTCCGTCGCTCTGGTTTGGGATCGCGAGAGATTAGGGTTCGAATTGGGGCTCGCTCTTCACTTATCCAAACCCACCTCCAATTCAAGGCTTCTCGACCTGGTGATATTGATGAGCGCGAGGTTGTTACTCCTGATGTCATGACCAAGTATTTCATAGGTGTCACACTAAAGGAAAGATCTCCAGACGGTGTTGGGAAGTTAGTTGATGTGGGCTTGAGTAAGAATGTCGTCGTTGATCAAGTACTTGATCCTGGAGCAAGAGTTACAGTGGCTATGGGAGACAGTTGGAATTTGGATGCTGGTTGTATCATCATCCAAGCCAAGGGAAGGGAAGAAGCAGGGATGTATTGGGGGTACAAAGTGCACTATGCTTCCAATATCACTTCGGTAATGATTGAATGTCCATACGAGGGTGGCTATGATCATTTGAATGGGACCTCAGAGCATGGTCAGATTATCAATTCCTCCGATCTCACTATACCCACTTTCAGGTATCTATTGATTGCGTTTGGTGGACTTGCTGGGTTGGAAGAGAGCATTGAAGAGGATCATAACTTAAAGGCAAAGAATGTGCGGGAGGAACAGCATGAGCTTCTCCAGAAAGTAGTGAAGCCTTTGACGGGTCGTTTAGAGATCTTAGCACCCAATGGTGGCCTTAACACTCACCGTTCTTCAGCAAACTCGTCAATCTACTCAGAATGTGTGTTTGTGTATTATCAAGTGCAGCAAACACTAGTGAAGTACTCACTCAAAACTTGTGTTCAACAGCAGCAAGCAATTAATGTCATTTTGAATGCATGGCCACAAGAAACTAATGATCGATGGCTTAAGAGATCGAAAGACTTCTTATCATCTTTGCCTACAAGAGAGCTTCTGCAAGAACAAATGAAGGGTTCAAAAGTAAGGCTTTTCCCACAACAATCATTGGTTGTTAGCCAATTTGATTACGATATTCACACCACAGCTAACCAAATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF502

Protein Analysis

361

Amino Acids

40.74

Weight (kDa)

5.95

Isoelectric Point (pI)

52.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltrn_RNA_3 PF02598 84 - 240 2.6e-31 Putative RNA methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 50
AccII CGCG 3 cut(s) 11, 133, 225
AciI CCGC 3 cut(s) 11, 67, 682
AclWI GGATC 3 cut(s) 136, 350, 663
AcoI YGGCCR 1 cut(s) 908
AcsI RAATTY 1 cut(s) 397
AcuI CTGAAG 1 cut(s) 765
AfaI GTAC 3 cut(s) 351, 464, 851
AfiI CCNNNNNNNGG 7 cut(s) 193, 430, 435, 518, 597, 728, 758
AflII CTTAAG 1 cut(s) 935
AgsI TTSAA 7 cut(s) 193, 506, 538, 650, 872, 901, 1005
AhlI ACTAGT 1 cut(s) 841
AjnI CCWGG 2 cut(s) 205, 358
AluBI AGCT 3 cut(s) 700, 979, 1073
AluI AGCT 3 cut(s) 700, 979, 1073
Alw21I GWGCWC 1 cut(s) 474
Alw26I GTCTC 1 cut(s) 381
Alw44I GTGCAC 1 cut(s) 470
AlwI GGATC 3 cut(s) 136, 350, 663
AoxI GGCC 2 cut(s) 760, 908
ApaLI GTGCAC 1 cut(s) 470
ApeKI GCWGC 2 cut(s) 832, 877
ApoI RAATTY 1 cut(s) 397
AseI ATTAAT 1 cut(s) 888
Asp700I GAANNNNTTC 1 cut(s) 950
AspLEI GCGC 1 cut(s) 225
AspS9I GGNCC 1 cut(s) 543
AsuHPI GGTGA 2 cut(s) 221, 764
AsuII TTCGAA 1 cut(s) 148
AvaII GGWCC 1 cut(s) 543
BaeGI GKGCMC 1 cut(s) 474
BalI TGGCCA 1 cut(s) 910
BanII GRGCYC 1 cut(s) 160
Bbv12I GWGCWC 1 cut(s) 474
BbvI GCAGC 2 cut(s) 844, 889
BccI CCATC 1 cut(s) 924
BciT130I CCWGG 2 cut(s) 207, 360
BclI TGATCA 2 cut(s) 343, 529
BcoDI GTCTC 1 cut(s) 381
BcuI ACTAGT 1 cut(s) 841
BfaI CTAG 1 cut(s) 842
BfrI CTTAAG 1 cut(s) 935
BglII AGATCT 2 cut(s) 284, 741
BisI GCNGC 3 cut(s) 12, 833, 878
BlsI GCNGC 3 cut(s) 13, 834, 879
BmcAI AGTACT 2 cut(s) 351, 851
Bme1390I CCNGG 2 cut(s) 207, 360
Bme18I GGWCC 1 cut(s) 543
BmgT120I GGNCC 1 cut(s) 543
BmiI GGNNCC 2 cut(s) 107, 544
BmrFI CCNGG 2 cut(s) 207, 360
BmsI GCATC 2 cut(s) 78, 394
BpmI CTGGAG 3 cut(s) 273, 381, 689
Bpu14I TTCGAA 1 cut(s) 148
BpuEI CTTGAG 1 cut(s) 343
Bsa29I ATCGAT 1 cut(s) 928
BsaJI CCNNGG 2 cut(s) 101, 429
BsaXI ACNNNNNCTCC 2 cut(s) 354, 384
Bsc4I CCNNNNNNNGG 7 cut(s) 193, 430, 435, 518, 597, 728, 758
BseBI CCWGG 2 cut(s) 207, 360
BseCI ATCGAT 1 cut(s) 928
BseDI CCNNGG 2 cut(s) 101, 429
BseGI GGATG 3 cut(s) 409, 420, 456
BseLI CCNNNNNNNGG 7 cut(s) 193, 430, 435, 518, 597, 728, 758
BseMII CTCAG 3 cut(s) 68, 561, 816
BseRI GAGGAG 1 cut(s) 98
BseSI GKGCMC 1 cut(s) 474
BseXI GCAGC 2 cut(s) 844, 889
BseYI CCCAGC 1 cut(s) 629
BsgI GTGCAG 2 cut(s) 70, 851
Bsh1236I CGCG 3 cut(s) 11, 133, 225
BshFI GGCC 2 cut(s) 762, 910
BshVI ATCGAT 1 cut(s) 928
BsiHKAI GWGCWC 1 cut(s) 474
BslFI GGGAC 2 cut(s) 47, 556
BslI CCNNNNNNNGG 7 cut(s) 193, 430, 435, 518, 597, 728, 758
BsmAI GTCTC 1 cut(s) 381
BsmFI GGGAC 2 cut(s) 47, 556
BsmI GAATGC 2 cut(s) 14, 907
BsnI GGCC 2 cut(s) 762, 910
Bsp119I TTCGAA 1 cut(s) 148
Bsp1286I GDGCHC 2 cut(s) 160, 474
Bsp68I TCGCGA 1 cut(s) 133
BspACI CCGC 3 cut(s) 11, 67, 682
BspANI GGCC 2 cut(s) 762, 910
BspCNI CTCAG 3 cut(s) 67, 560, 815
BspDI ATCGAT 1 cut(s) 928
BspFNI CGCG 3 cut(s) 11, 133, 225
BspHI TCATGA 1 cut(s) 246
BspLI GGNNCC 2 cut(s) 107, 544
BspPI GGATC 3 cut(s) 136, 350, 663
BspQI GCTCTTC 1 cut(s) 169
BspT104I TTCGAA 1 cut(s) 148
BspTI CTTAAG 1 cut(s) 935
BssECI CCNNGG 2 cut(s) 101, 429
BssT1I CCWWGG 1 cut(s) 429
Bst2UI CCWGG 2 cut(s) 207, 360
Bst4CI ACNGT 4 cut(s) 296, 376, 392, 776
Bst6I CTCTTC 3 cut(s) 169, 633, 645
BstAFI CTTAAG 1 cut(s) 935
BstBI TTCGAA 1 cut(s) 148
BstC8I GCNNGC 2 cut(s) 160, 882
BstDEI CTNAG 4 cut(s) 54, 547, 745, 802
BstF5I GGATG 3 cut(s) 409, 420, 456
BstFNI CGCG 3 cut(s) 11, 133, 225
BstHHI GCGC 1 cut(s) 225
BstMAI GTCTC 1 cut(s) 381
BstNI CCWGG 2 cut(s) 207, 360
BstSCI CCNGG 2 cut(s) 205, 358
BstSLI GKGCMC 1 cut(s) 474
BstUI CGCG 3 cut(s) 11, 133, 225
BstV1I GCAGC 2 cut(s) 844, 889
BstX2I RGATCY 2 cut(s) 284, 741
BstYI RGATCY 2 cut(s) 284, 741
Bsu15I ATCGAT 1 cut(s) 928
BsuRI GGCC 2 cut(s) 762, 910
BsuTUI ATCGAT 1 cut(s) 928
BtsCI GGATG 3 cut(s) 409, 420, 456
BtsIMutI CAGTG 2 cut(s) 62, 381
BtuMI TCGCGA 1 cut(s) 133
Cac8I GCNNGC 2 cut(s) 160, 882
CciI TCATGA 1 cut(s) 246
CfoI GCGC 1 cut(s) 225
Cfr13I GGNCC 1 cut(s) 543
ClaI ATCGAT 1 cut(s) 928
CsiI ACCWGGT 1 cut(s) 205
Csp6I GTAC 3 cut(s) 350, 463, 850
CviAII CATG 4 cut(s) 247, 554, 695, 906
CviQI GTAC 3 cut(s) 350, 463, 850
DdeI CTNAG 4 cut(s) 54, 547, 745, 802
EaeI YGGCCR 1 cut(s) 908
Eam1104I CTCTTC 3 cut(s) 169, 633, 645
EarI CTCTTC 3 cut(s) 169, 633, 645
Eco130I CCWWGG 1 cut(s) 429
Eco24I GRGCYC 1 cut(s) 160
Eco47I GGWCC 1 cut(s) 543
Eco57I CTGAAG 1 cut(s) 765
EcoO109I RGGNCCY 1 cut(s) 543
EcoRII CCWGG 2 cut(s) 205, 358
EcoT14I CCWWGG 1 cut(s) 429
EcoT22I ATGCAT 1 cut(s) 907
EcoT38I GRGCYC 1 cut(s) 160
ErhI CCWWGG 1 cut(s) 429
FaeI CATG 4 cut(s) 250, 557, 698, 909
FalI AAGNNNNNCTT 2 cut(s) 999, 1031
FaqI GGGAC 2 cut(s) 47, 556
FatI CATG 4 cut(s) 246, 553, 694, 905
FauI CCCGC 1 cut(s) 675
FbaI TGATCA 2 cut(s) 343, 529
FblI GTMKAC 1 cut(s) 50
Fnu4HI GCNGC 3 cut(s) 12, 833, 878
FokI GGATG 3 cut(s) 407, 416, 463
FriOI GRGCYC 1 cut(s) 160
Fsp4HI GCNGC 3 cut(s) 12, 833, 878
FspBI CTAG 1 cut(s) 842
GlaI GCGC 1 cut(s) 224
GluI GCNGC 3 cut(s) 12, 833, 878
GsaI CCCAGC 1 cut(s) 633
GsuI CTGGAG 3 cut(s) 273, 381, 689
HaeIII GGCC 2 cut(s) 762, 910
HhaI GCGC 1 cut(s) 225
Hin1II CATG 4 cut(s) 250, 557, 698, 909
Hin6I GCGC 1 cut(s) 223
HinP1I GCGC 1 cut(s) 223
HincII GTYRAC 1 cut(s) 51
HindII GTYRAC 1 cut(s) 51
HinfI GANTC 2 cut(s) 47, 52
HphI GGTGA 2 cut(s) 221, 764
Hpy166II GTNNAC 3 cut(s) 51, 472, 623
Hpy188I TCNGA 4 cut(s) 550, 561, 577, 805
Hpy188III TCNNGA 6 cut(s) 132, 201, 239, 247, 290, 706
Hpy8I GTNNAC 3 cut(s) 51, 472, 623
Hpy99I CGWCG 2 cut(s) 117, 341
HpyAV CCTTC 2 cut(s) 429, 991
HpyCH4III ACNGT 4 cut(s) 296, 376, 392, 776
HpyCH4V TGCA 5 cut(s) 87, 472, 832, 905, 985
HpyF3I CTNAG 4 cut(s) 54, 547, 745, 802
Hsp92II CATG 4 cut(s) 250, 557, 698, 909
HspAI GCGC 1 cut(s) 223
Ksp22I TGATCA 2 cut(s) 343, 529
LguI GCTCTTC 1 cut(s) 169
LmnI GCTCC 2 cut(s) 111, 362
Lsp1109I GCAGC 2 cut(s) 844, 889
LweI GCATC 2 cut(s) 78, 394
MabI ACCWGGT 1 cut(s) 205
MaeI CTAG 1 cut(s) 842
MaeIII GTNAC 3 cut(s) 232, 268, 370
MboII GAAGA 6 cut(s) 64, 156, 452, 650, 662, 771
MflI RGATCY 2 cut(s) 284, 741
MhlI GDGCHC 2 cut(s) 160, 474
MlsI TGGCCA 1 cut(s) 910
MluCI AATT 6 cut(s) 150, 188, 397, 568, 885, 1046
MluNI TGGCCA 1 cut(s) 910
MlyI GAGTC 2 cut(s) 46, 56
MmeI TCCRAC 2 cut(s) 374, 615
MnlI CCTC 9 cut(s) 111, 119, 194, 220, 511, 556, 583, 646, 679
Mox20I TGGCCA 1 cut(s) 910
Mph1103I ATGCAT 1 cut(s) 907
MroXI GAANNNNTTC 1 cut(s) 950
MscI TGGCCA 1 cut(s) 910
MseI TTAA 4 cut(s) 665, 765, 888, 936
MslI CAYNNNNRTG 1 cut(s) 537
Msp20I TGGCCA 1 cut(s) 910
MspCI CTTAAG 1 cut(s) 935
MspR9I CCNGG 2 cut(s) 207, 360
Mva1269I GAATGC 2 cut(s) 14, 907
MvaI CCWGG 2 cut(s) 207, 360
MvnI CGCG 3 cut(s) 11, 133, 225
NlaIII CATG 4 cut(s) 250, 557, 698, 909
NlaIV GGNNCC 2 cut(s) 107, 544
NmeAIII GCCGAG 1 cut(s) 82
NmuCI GTSAC 1 cut(s) 268
NruI TCGCGA 1 cut(s) 133
NsiI ATGCAT 1 cut(s) 907
NspV TTCGAA 1 cut(s) 148
PagI TCATGA 1 cut(s) 246
PciSI GCTCTTC 1 cut(s) 169
PcsI WCGNNNNNNNCGW 3 cut(s) 15, 42, 109
PctI GAATGC 2 cut(s) 14, 907
PdmI GAANNNNTTC 1 cut(s) 950
PfoI TCCNGGA 1 cut(s) 358
PkrI GCNGC 3 cut(s) 13, 834, 879
PleI GAGTC 2 cut(s) 46, 55
PpsI GAGTC 2 cut(s) 46, 55
PpuMI RGGWCCY 1 cut(s) 543
PshBI ATTAAT 1 cut(s) 888
Psp5II RGGWCCY 1 cut(s) 543
Psp6I CCWGG 2 cut(s) 205, 358
PspFI CCCAGC 1 cut(s) 629
PspGI CCWGG 2 cut(s) 205, 358
PspN4I GGNNCC 2 cut(s) 107, 544
PspPI GGNCC 1 cut(s) 543
PspPPI RGGWCCY 1 cut(s) 543
PsuI RGATCY 2 cut(s) 284, 741
RruI TCGCGA 1 cut(s) 133
RsaI GTAC 3 cut(s) 351, 464, 851
RsaNI GTAC 3 cut(s) 350, 463, 850
RseI CAYNNNNRTG 1 cut(s) 537
SalI GTCGAC 1 cut(s) 49
SapI GCTCTTC 1 cut(s) 169
SaqAI TTAA 4 cut(s) 665, 765, 888, 936
SatI GCNGC 3 cut(s) 12, 833, 878
Sau96I GGNCC 1 cut(s) 543
ScaI AGTACT 2 cut(s) 351, 851
SchI GAGTC 2 cut(s) 46, 56
ScrFI CCNGG 2 cut(s) 207, 360
SduI GDGCHC 2 cut(s) 160, 474
SexAI ACCWGGT 1 cut(s) 205
SfaNI GCATC 2 cut(s) 78, 394
SfuI TTCGAA 1 cut(s) 148
SinI GGWCC 1 cut(s) 543
SmiMI CAYNNNNRTG 1 cut(s) 537
SmlI CTYRAG 2 cut(s) 322, 935
SmoI CTYRAG 2 cut(s) 322, 935
SpeI ACTAGT 1 cut(s) 841
Sse9I AATT 6 cut(s) 150, 188, 397, 568, 885, 1046
SsiI CCGC 3 cut(s) 11, 67, 682
SspMI CTAG 1 cut(s) 842
StyD4I CCNGG 2 cut(s) 205, 358
StyI CCWWGG 1 cut(s) 429
TaaI ACNGT 4 cut(s) 296, 376, 392, 776
TaqI TCGA 5 cut(s) 50, 148, 202, 928, 944
TasI AATT 6 cut(s) 150, 188, 397, 568, 885, 1046
TatI WGTACW 2 cut(s) 349, 849
TauI GCSGC 1 cut(s) 14
Tru1I TTAA 4 cut(s) 665, 765, 888, 936
Tru9I TTAA 4 cut(s) 665, 765, 888, 936
TscAI CASTG 2 cut(s) 62, 381
TseFI GTSAC 1 cut(s) 268
TseI GCWGC 2 cut(s) 832, 877
Tsp45I GTSAC 1 cut(s) 268
TspDTI ATGAA 2 cut(s) 250, 1010
TspGWI ACGGA 1 cut(s) 101
TspRI CASTG 2 cut(s) 62, 381
Vha464I CTTAAG 1 cut(s) 935
VneI GTGCAC 1 cut(s) 470
VpaK11BI GGWCC 1 cut(s) 543
VspI ATTAAT 1 cut(s) 888
XapI RAATTY 1 cut(s) 397
XmiI GTMKAC 1 cut(s) 50
XmnI GAANNNNTTC 1 cut(s) 950
XspI CTAG 1 cut(s) 842
ZrmI AGTACT 2 cut(s) 351, 851
Zsp2I ATGCAT 1 cut(s) 907
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.