Rh2BG294400

Protein of unknown function (DUF502)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0002755
Physical Location & Seq
Reverse (-)
33425883 .. 33430585
4703 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG294400.1

Sequence Viewer

Length: 678 bp
ATGGCGGAAGATAAGGAGTCGACGTCGATTCCACTGAGTCAAGCTGAGAACGGCGGTCCCGATCCCGAAGACCCGGCCAAGTCTCCTCCAAGCTCTCCCAATTCCTCCACTCGTAAGGCTTGTTGTTTTGTTCTCCAGAGTTGGGTGTCTAAGAAGTTTATGACTGGAATTGTAGTTCTTTTCCCTGTTGCTGTTACTTTTTTCATTACGTGGTGGTTTGTCCAGTTTGTTGATGGGTTCTTCAGTCCACTCTACGAGCAGCTTGGCATTGAGATCTTCGGACTCGGATTTATCACATCCTTACTTTTCGTATTCTTTGTTGGTGTCTTTGTTTCCTCATGGATGGGTGCCACTGTTTTCTCTCTTGGAGAATGGATTATAAAGAAAATGCCTTTTGTTAAGCATATCTACTCAGCCTCCAAACAAATTAGTTCCGCAATTTCTCCAGACCAAAATACCACAGCTTTTAAAGAGGTAGCAATTATCCGTCACCCACGTGTTGGCGAATATGCTTTTGGGTTTATTACATCAACCGTTACCCTTCAGGTTCTCATCTTTATGTTTCTATCCATTTGTTTCCTGTCTAAAATAGTCTTGTGGTTGATATTCTGTATTTCAAATCAAAGAGTTAAATTTATTTGCTCAAATACGTCTGACTTTTCAATTCATTTGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF502

Protein Analysis

225

Amino Acids

25.24

Weight (kDa)

6.82

Isoelectric Point (pI)

40.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF502 PF04367 99 - 179 5.6e-22 Protein of unknown function (DUF502)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 380
AatII GACGTC 1 cut(s) 26
AccB1I GGYRCC 1 cut(s) 347
AccB7I CCANNNNNTGG 1 cut(s) 500
AccI GTMKAC 1 cut(s) 20
AciI CCGC 3 cut(s) 5, 54, 435
AclWI GGATC 1 cut(s) 56
AcoI YGGCCR 1 cut(s) 75
AcsI RAATTY 1 cut(s) 632
AcuI CTGAAG 2 cut(s) 226, 527
AcvI CACGTG 1 cut(s) 497
AcyI GRCGYC 1 cut(s) 23
AfiI CCNNNNNNNGG 2 cut(s) 142, 500
AflIII ACRYGT 1 cut(s) 496
AgsI TTSAA 2 cut(s) 618, 663
AjuI GAANNNNNNNTTGG 2 cut(s) 498, 530
AleI CACNNNNGTG 1 cut(s) 495
AloI GAACNNNNNNTCC 2 cut(s) 159, 191
AluBI AGCT 4 cut(s) 44, 93, 262, 464
AluI AGCT 4 cut(s) 44, 93, 262, 464
Alw26I GTCTC 1 cut(s) 87
AlwI GGATC 1 cut(s) 56
AoxI GGCC 1 cut(s) 75
ApeKI GCWGC 1 cut(s) 259
ApoI RAATTY 1 cut(s) 632
AspS9I GGNCC 1 cut(s) 56
AsuC2I CCSGG 1 cut(s) 74
AsuHPI GGTGA 1 cut(s) 482
AvaII GGWCC 1 cut(s) 56
BanI GGYRCC 1 cut(s) 347
BbrPI CACGTG 1 cut(s) 497
BbsI GAAGAC 1 cut(s) 75
BbvI GCAGC 1 cut(s) 271
BccI CCATC 2 cut(s) 227, 337
BceAI ACGGC 1 cut(s) 67
BcnI CCSGG 1 cut(s) 74
BcoDI GTCTC 1 cut(s) 87
BglII AGATCT 1 cut(s) 273
BisI GCNGC 1 cut(s) 260
BlsI GCNGC 1 cut(s) 261
Bme1390I CCNGG 1 cut(s) 74
Bme18I GGWCC 1 cut(s) 56
BmgT120I GGNCC 1 cut(s) 56
BmiI GGNNCC 2 cut(s) 58, 349
BmrFI CCNGG 1 cut(s) 74
BpiI GAAGAC 1 cut(s) 75
BpmI CTGGAG 2 cut(s) 119, 429
BpuMI CCSGG 1 cut(s) 74
BsaAI YACGTR 2 cut(s) 210, 497
BsaHI GRCGYC 1 cut(s) 23
BsaXI ACNNNNNCTCC 4 cut(s) 8, 38, 401, 431
Bsc4I CCNNNNNNNGG 2 cut(s) 142, 500
Bse1I ACTGG 2 cut(s) 169, 223
BseGI GGATG 2 cut(s) 296, 348
BseLI CCNNNNNNNGG 2 cut(s) 142, 500
BseMII CTCAG 3 cut(s) 26, 36, 426
BseNI ACTGG 2 cut(s) 169, 223
BseRI GAGGAG 1 cut(s) 75
BseXI GCAGC 1 cut(s) 271
BshFI GGCC 1 cut(s) 77
BshNI GGYRCC 1 cut(s) 347
BsiSI CCGG 1 cut(s) 74
BslFI GGGAC 1 cut(s) 42
BslI CCNNNNNNNGG 2 cut(s) 142, 500
BsmAI GTCTC 1 cut(s) 87
BsmFI GGGAC 1 cut(s) 42
BsnI GGCC 1 cut(s) 77
Bsp143I GATC 2 cut(s) 61, 273
BspACI CCGC 3 cut(s) 5, 54, 435
BspANI GGCC 1 cut(s) 77
BspCNI CTCAG 3 cut(s) 27, 37, 425
BspLI GGNNCC 2 cut(s) 58, 349
BspPI GGATC 1 cut(s) 56
BspT107I GGYRCC 1 cut(s) 347
BsrI ACTGG 2 cut(s) 169, 223
BssMI GATC 2 cut(s) 61, 273
BssNI GRCGYC 1 cut(s) 23
Bst4CI ACNGT 2 cut(s) 355, 535
BstACI GRCGYC 1 cut(s) 23
BstBAI YACGTR 2 cut(s) 210, 497
BstDEI CTNAG 4 cut(s) 35, 45, 150, 412
BstF5I GGATG 2 cut(s) 296, 348
BstKTI GATC 2 cut(s) 64, 276
BstMAI GTCTC 1 cut(s) 87
BstMBI GATC 2 cut(s) 61, 273
BstSCI CCNGG 1 cut(s) 72
BstV1I GCAGC 1 cut(s) 271
BstV2I GAAGAC 1 cut(s) 75
BstX2I RGATCY 1 cut(s) 273
BstYI RGATCY 1 cut(s) 273
BsuRI GGCC 1 cut(s) 77
BtsCI GGATG 2 cut(s) 296, 348
BtsIMutI CAGTG 2 cut(s) 32, 351
Cfr13I GGNCC 1 cut(s) 56
CviAII CATG 1 cut(s) 339
CviJI RGCY 7 cut(s) 44, 77, 93, 119, 262, 416, 464
CviKI_1 RGCY 7 cut(s) 44, 77, 93, 119, 262, 416, 464
DdeI CTNAG 4 cut(s) 35, 45, 150, 412
DpnI GATC 2 cut(s) 63, 275
DpnII GATC 2 cut(s) 61, 273
DraI TTTAAA 1 cut(s) 469
EaeI YGGCCR 1 cut(s) 75
EciI GGCGGA 1 cut(s) 20
Eco47I GGWCC 1 cut(s) 56
Eco57I CTGAAG 2 cut(s) 226, 527
Eco72I CACGTG 1 cut(s) 497
FaeI CATG 1 cut(s) 342
FaiI YATR 6 cut(s) 161, 340, 380, 405, 510, 560
FaqI GGGAC 1 cut(s) 42
FatI CATG 1 cut(s) 338
FblI GTMKAC 1 cut(s) 20
Fnu4HI GCNGC 1 cut(s) 260
FokI GGATG 2 cut(s) 283, 355
Fsp4HI GCNGC 1 cut(s) 260
GluI GCNGC 1 cut(s) 260
GsuI CTGGAG 2 cut(s) 119, 429
HaeIII GGCC 1 cut(s) 77
HapII CCGG 1 cut(s) 74
Hin1I GRCGYC 1 cut(s) 23
Hin1II CATG 1 cut(s) 342
HincII GTYRAC 1 cut(s) 21
HindII GTYRAC 1 cut(s) 21
HinfI GANTC 4 cut(s) 17, 28, 37, 282
HpaII CCGG 1 cut(s) 74
HphI GGTGA 1 cut(s) 482
Hpy166II GTNNAC 2 cut(s) 21, 248
Hpy188I TCNGA 3 cut(s) 281, 287, 655
Hpy188III TCNNGA 4 cut(s) 59, 65, 136, 446
Hpy8I GTNNAC 2 cut(s) 21, 248
Hpy99I CGWCG 2 cut(s) 25, 28
HpyAV CCTTC 1 cut(s) 551
HpyCH4III ACNGT 2 cut(s) 355, 535
HpyCH4IV ACGT 4 cut(s) 23, 209, 496, 650
HpyF3I CTNAG 4 cut(s) 35, 45, 150, 412
HpySE526I ACGT 4 cut(s) 23, 209, 496, 650
Hsp92I GRCGYC 1 cut(s) 23
Hsp92II CATG 1 cut(s) 342
Kzo9I GATC 2 cut(s) 61, 273
LpnPI CCDG 8 cut(s) 87, 149, 150, 198, 236, 459, 530, 593
Lsp1109I GCAGC 1 cut(s) 271
MaeII ACGT 4 cut(s) 23, 209, 496, 650
MaeIII GTNAC 3 cut(s) 193, 488, 535
MalI GATC 2 cut(s) 63, 275
MboI GATC 2 cut(s) 61, 273
MboII GAAGA 4 cut(s) 20, 80, 232, 268
MflI RGATCY 1 cut(s) 273
MluCI AATT 7 cut(s) 100, 168, 426, 438, 480, 632, 663
MlyI GAGTC 3 cut(s) 26, 46, 276
MnlI CCTC 5 cut(s) 96, 115, 346, 427, 466
MseI TTAA 3 cut(s) 399, 468, 630
MslI CAYNNNNRTG 2 cut(s) 495, 557
MspI CCGG 1 cut(s) 74
MspR9I CCNGG 1 cut(s) 74
NciI CCSGG 1 cut(s) 74
NdeII GATC 2 cut(s) 61, 273
NlaIII CATG 1 cut(s) 342
NlaIV GGNNCC 2 cut(s) 58, 349
NmuCI GTSAC 1 cut(s) 488
OliI CACNNNNGTG 1 cut(s) 495
PcsI WCGNNNNNNNCGW 1 cut(s) 57
PfeI GAWTC 1 cut(s) 28
PflMI CCANNNNNTGG 1 cut(s) 500
PkrI GCNGC 1 cut(s) 261
PleI GAGTC 3 cut(s) 25, 45, 276
PmaCI CACGTG 1 cut(s) 497
PmlI CACGTG 1 cut(s) 497
PpsI GAGTC 3 cut(s) 25, 45, 276
Ppu21I YACGTR 2 cut(s) 210, 497
PsiI TTATAA 1 cut(s) 380
PspCI CACGTG 1 cut(s) 497
PspN4I GGNNCC 2 cut(s) 58, 349
PspPI GGNCC 1 cut(s) 56
PsuI RGATCY 1 cut(s) 273
RseI CAYNNNNRTG 2 cut(s) 495, 557
SalI GTCGAC 1 cut(s) 19
SaqAI TTAA 3 cut(s) 399, 468, 630
SatI GCNGC 1 cut(s) 260
Sau3AI GATC 2 cut(s) 61, 273
Sau96I GGNCC 1 cut(s) 56
SchI GAGTC 3 cut(s) 26, 46, 276
ScrFI CCNGG 1 cut(s) 74
SinI GGWCC 1 cut(s) 56
SmiMI CAYNNNNRTG 2 cut(s) 495, 557
Sse9I AATT 7 cut(s) 100, 168, 426, 438, 480, 632, 663
SsiI CCGC 3 cut(s) 5, 54, 435
StyD4I CCNGG 1 cut(s) 72
TaaI ACNGT 2 cut(s) 355, 535
TaiI ACGT 4 cut(s) 26, 212, 499, 653
TaqI TCGA 2 cut(s) 20, 26
TasI AATT 7 cut(s) 100, 168, 426, 438, 480, 632, 663
TfiI GAWTC 1 cut(s) 28
Tru1I TTAA 3 cut(s) 399, 468, 630
Tru9I TTAA 3 cut(s) 399, 468, 630
TscAI CASTG 2 cut(s) 39, 358
TseFI GTSAC 1 cut(s) 488
TseI GCWGC 1 cut(s) 259
Tsp45I GTSAC 1 cut(s) 488
TspDTI ATGAA 2 cut(s) 193, 656
TspGWI ACGGA 1 cut(s) 476
TspRI CASTG 2 cut(s) 39, 358
Van91I CCANNNNNTGG 1 cut(s) 500
VpaK11BI GGWCC 1 cut(s) 56
XapI RAATTY 1 cut(s) 632
XcmI CCANNNNNNNNNTGG 1 cut(s) 230
XmiI GTMKAC 1 cut(s) 20
ZraI GACGTC 1 cut(s) 24
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.