Rroxscaffold_2G00123760

Protein of unknown function (DUF502)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0002755
Physical Location & Seq
Forward (+)
57647120 .. 57752542
105423 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00123760.1

Sequence Viewer

Length: 774 bp
ATGGCGGAAGATAAGGAGTCGACGTCGATTCCACTGAGTCAAGCTGAGAACGGCGGTCCCGATCCCGAAGACCCGGCCAAGTCTCCTCCAAGCTCTCCCAATTCCTCCACTCGTAAGGCTTGTTGTTTTGTTCTCCAGAGTTGGGTGTCTAAGAAGTTTATGACTGGAATTGTAGTTCTTTTCCCTGTTGCTGTTACTTTTTTCATTACGTGGTGGTTCGTCCAGTTTGTTGATGGGTTCTTCAGTCCACTCTACGAGCAGCTTGGCATTGAGATCTTCGGACTTGGATTTATCACATCCTTACTTTTTGTATTCTTTGTTGGTGTATTTGTTTCCTCATGGATGGGTGCCACTGTTTTCTCTCTTGGAGAATGGATTATAAAGAAAATGCCTTTTGTTAAACATATCTACTCAGCCTCCAAACAAATTAGTTCCGCAATTTCTCCAGACCAAAATACCACAGCTTTTAAAGAGGTAGCAATTATCCGTCACCCACGTGTTGGTGAATATGCTTTTGGGTTTATTACATCAACTGTTACCCTTCAGAGAGATAACGAAGATGAAGAATTATGTAGTGTTTTTGTCCCAACAAATCATCTATATATAGGTGACATATTTCTCGTTAACTCCAAAGATATCATAAGACCAAATCTGTCTATCCGAGAAGGCATAGAGATCATTGTTTCTGGGGGTATGACAATGCCTCAAATCATTTCTCCTCAAGAAAGGGTTACCCAACAGAGTGATAAAATTCCTTTGAACAGAATAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF502

Protein Analysis

257

Amino Acids

28.71

Weight (kDa)

5.46

Isoelectric Point (pI)

48.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF502 PF04367 99 - 199 9.5e-28 Protein of unknown function (DUF502)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 380
AatII GACGTC 1 cut(s) 26
AccB1I GGYRCC 1 cut(s) 347
AccB7I CCANNNNNTGG 1 cut(s) 500
AccI GTMKAC 1 cut(s) 20
AciI CCGC 3 cut(s) 5, 54, 435
AclWI GGATC 1 cut(s) 56
AcoI YGGCCR 1 cut(s) 75
AcsI RAATTY 1 cut(s) 750
AcuI CTGAAG 2 cut(s) 226, 527
AcvI CACGTG 1 cut(s) 497
AcyI GRCGYC 1 cut(s) 23
AfiI CCNNNNNNNGG 2 cut(s) 142, 500
AflIII ACRYGT 1 cut(s) 496
AgsI TTSAA 1 cut(s) 760
AjuI GAANNNNNNNTTGG 2 cut(s) 498, 530
AleI CACNNNNGTG 1 cut(s) 495
AloI GAACNNNNNNTCC 2 cut(s) 159, 191
AluBI AGCT 4 cut(s) 44, 93, 262, 464
AluI AGCT 4 cut(s) 44, 93, 262, 464
Alw26I GTCTC 1 cut(s) 87
AlwI GGATC 1 cut(s) 56
AoxI GGCC 1 cut(s) 75
ApeKI GCWGC 1 cut(s) 259
ApoI RAATTY 1 cut(s) 750
AspS9I GGNCC 1 cut(s) 56
AsuC2I CCSGG 1 cut(s) 74
AsuHPI GGTGA 3 cut(s) 482, 515, 620
AvaII GGWCC 1 cut(s) 56
BanI GGYRCC 1 cut(s) 347
BbrPI CACGTG 1 cut(s) 497
BbsI GAAGAC 1 cut(s) 75
BbvI GCAGC 1 cut(s) 271
BccI CCATC 2 cut(s) 227, 337
BceAI ACGGC 1 cut(s) 67
BcnI CCSGG 1 cut(s) 74
BcoDI GTCTC 1 cut(s) 87
BglII AGATCT 1 cut(s) 273
BisI GCNGC 1 cut(s) 260
BlsI GCNGC 1 cut(s) 261
Bme1390I CCNGG 1 cut(s) 74
Bme18I GGWCC 1 cut(s) 56
BmgT120I GGNCC 1 cut(s) 56
BmiI GGNNCC 2 cut(s) 58, 349
BmrFI CCNGG 1 cut(s) 74
BpiI GAAGAC 1 cut(s) 75
BpmI CTGGAG 2 cut(s) 119, 429
BpuEI CTTGAG 1 cut(s) 705
BpuMI CCSGG 1 cut(s) 74
BsaAI YACGTR 2 cut(s) 210, 497
BsaHI GRCGYC 1 cut(s) 23
BsaXI ACNNNNNCTCC 4 cut(s) 8, 38, 401, 431
Bsc4I CCNNNNNNNGG 2 cut(s) 142, 500
Bse1I ACTGG 2 cut(s) 169, 223
BseGI GGATG 2 cut(s) 296, 348
BseLI CCNNNNNNNGG 2 cut(s) 142, 500
BseMII CTCAG 3 cut(s) 26, 36, 426
BseNI ACTGG 2 cut(s) 169, 223
BseRI GAGGAG 2 cut(s) 75, 708
BseXI GCAGC 1 cut(s) 271
BshFI GGCC 1 cut(s) 77
BshNI GGYRCC 1 cut(s) 347
BsiSI CCGG 1 cut(s) 74
BslFI GGGAC 2 cut(s) 42, 569
BslI CCNNNNNNNGG 2 cut(s) 142, 500
BsmAI GTCTC 1 cut(s) 87
BsmFI GGGAC 2 cut(s) 42, 569
BsnI GGCC 1 cut(s) 77
Bsp143I GATC 3 cut(s) 61, 273, 675
BspACI CCGC 3 cut(s) 5, 54, 435
BspANI GGCC 1 cut(s) 77
BspCNI CTCAG 3 cut(s) 27, 37, 425
BspLI GGNNCC 2 cut(s) 58, 349
BspPI GGATC 1 cut(s) 56
BspT107I GGYRCC 1 cut(s) 347
BsrI ACTGG 2 cut(s) 169, 223
BssMI GATC 3 cut(s) 61, 273, 675
BssNI GRCGYC 1 cut(s) 23
Bst4CI ACNGT 2 cut(s) 355, 535
BstACI GRCGYC 1 cut(s) 23
BstBAI YACGTR 2 cut(s) 210, 497
BstDEI CTNAG 4 cut(s) 35, 45, 150, 412
BstEII GGTNACC 1 cut(s) 730
BstF5I GGATG 2 cut(s) 296, 348
BstKTI GATC 3 cut(s) 64, 276, 678
BstMAI GTCTC 1 cut(s) 87
BstMBI GATC 3 cut(s) 61, 273, 675
BstPI GGTNACC 1 cut(s) 730
BstSCI CCNGG 1 cut(s) 72
BstV1I GCAGC 1 cut(s) 271
BstV2I GAAGAC 1 cut(s) 75
BstX2I RGATCY 1 cut(s) 273
BstYI RGATCY 1 cut(s) 273
BsuRI GGCC 1 cut(s) 77
BtsCI GGATG 2 cut(s) 296, 348
BtsIMutI CAGTG 2 cut(s) 32, 351
Cfr13I GGNCC 1 cut(s) 56
CviAII CATG 1 cut(s) 339
CviJI RGCY 7 cut(s) 44, 77, 93, 119, 262, 416, 464
CviKI_1 RGCY 7 cut(s) 44, 77, 93, 119, 262, 416, 464
DdeI CTNAG 4 cut(s) 35, 45, 150, 412
DpnI GATC 3 cut(s) 63, 275, 677
DpnII GATC 3 cut(s) 61, 273, 675
DraI TTTAAA 1 cut(s) 469
EaeI YGGCCR 1 cut(s) 75
EciI GGCGGA 1 cut(s) 20
Eco32I GATATC 1 cut(s) 637
Eco47I GGWCC 1 cut(s) 56
Eco57I CTGAAG 2 cut(s) 226, 527
Eco72I CACGTG 1 cut(s) 497
Eco91I GGTNACC 1 cut(s) 730
EcoO65I GGTNACC 1 cut(s) 730
EcoRV GATATC 1 cut(s) 637
FaeI CATG 1 cut(s) 342
FaqI GGGAC 2 cut(s) 42, 569
FatI CATG 1 cut(s) 338
FblI GTMKAC 1 cut(s) 20
Fnu4HI GCNGC 1 cut(s) 260
FokI GGATG 2 cut(s) 283, 355
Fsp4HI GCNGC 1 cut(s) 260
GluI GCNGC 1 cut(s) 260
GsuI CTGGAG 2 cut(s) 119, 429
HaeIII GGCC 1 cut(s) 77
HapII CCGG 1 cut(s) 74
Hin1I GRCGYC 1 cut(s) 23
Hin1II CATG 1 cut(s) 342
HincII GTYRAC 2 cut(s) 21, 625
HindII GTYRAC 2 cut(s) 21, 625
HinfI GANTC 3 cut(s) 17, 28, 37
HpaI GTTAAC 1 cut(s) 625
HpaII CCGG 1 cut(s) 74
HphI GGTGA 3 cut(s) 482, 515, 620
Hpy166II GTNNAC 3 cut(s) 21, 248, 625
Hpy188I TCNGA 3 cut(s) 281, 546, 662
Hpy188III TCNNGA 5 cut(s) 59, 65, 136, 446, 722
Hpy8I GTNNAC 3 cut(s) 21, 248, 625
Hpy99I CGWCG 2 cut(s) 25, 28
HpyAV CCTTC 2 cut(s) 551, 659
HpyCH4III ACNGT 2 cut(s) 355, 535
HpyCH4IV ACGT 3 cut(s) 23, 209, 496
HpyF3I CTNAG 4 cut(s) 35, 45, 150, 412
HpySE526I ACGT 3 cut(s) 23, 209, 496
Hsp92I GRCGYC 1 cut(s) 23
Hsp92II CATG 1 cut(s) 342
KspAI GTTAAC 1 cut(s) 625
Kzo9I GATC 3 cut(s) 61, 273, 675
LpnPI CCDG 7 cut(s) 87, 149, 150, 198, 236, 459, 672
Lsp1109I GCAGC 1 cut(s) 271
MaeII ACGT 3 cut(s) 23, 209, 496
MaeIII GTNAC 5 cut(s) 193, 488, 535, 608, 730
MalI GATC 3 cut(s) 63, 275, 677
MboI GATC 3 cut(s) 61, 273, 675
MboII GAAGA 6 cut(s) 20, 80, 232, 268, 569, 575
MflI RGATCY 1 cut(s) 273
MluCI AATT 7 cut(s) 100, 168, 426, 438, 480, 566, 750
MlyI GAGTC 2 cut(s) 26, 46
MnlI CCTC 7 cut(s) 96, 115, 346, 427, 466, 714, 729
MseI TTAA 3 cut(s) 399, 468, 624
MslI CAYNNNNRTG 1 cut(s) 495
MspI CCGG 1 cut(s) 74
MspR9I CCNGG 1 cut(s) 74
NciI CCSGG 1 cut(s) 74
NdeII GATC 3 cut(s) 61, 273, 675
NlaIII CATG 1 cut(s) 342
NlaIV GGNNCC 2 cut(s) 58, 349
NmuCI GTSAC 2 cut(s) 488, 608
OliI CACNNNNGTG 1 cut(s) 495
PcsI WCGNNNNNNNCGW 1 cut(s) 57
PfeI GAWTC 1 cut(s) 28
PflMI CCANNNNNTGG 1 cut(s) 500
PkrI GCNGC 1 cut(s) 261
PleI GAGTC 2 cut(s) 25, 45
PmaCI CACGTG 1 cut(s) 497
PmlI CACGTG 1 cut(s) 497
PpsI GAGTC 2 cut(s) 25, 45
Ppu21I YACGTR 2 cut(s) 210, 497
PsiI TTATAA 1 cut(s) 380
PspCI CACGTG 1 cut(s) 497
PspEI GGTNACC 1 cut(s) 730
PspN4I GGNNCC 2 cut(s) 58, 349
PspPI GGNCC 1 cut(s) 56
PsuI RGATCY 1 cut(s) 273
RseI CAYNNNNRTG 1 cut(s) 495
SalI GTCGAC 1 cut(s) 19
SaqAI TTAA 3 cut(s) 399, 468, 624
SatI GCNGC 1 cut(s) 260
Sau3AI GATC 3 cut(s) 61, 273, 675
Sau96I GGNCC 1 cut(s) 56
SchI GAGTC 2 cut(s) 26, 46
ScrFI CCNGG 1 cut(s) 74
SetI ASST 9 cut(s) 26, 46, 95, 212, 264, 466, 477, 499, 610
SinI GGWCC 1 cut(s) 56
SmiMI CAYNNNNRTG 1 cut(s) 495
SmlI CTYRAG 1 cut(s) 720
SmoI CTYRAG 1 cut(s) 720
Sse9I AATT 7 cut(s) 100, 168, 426, 438, 480, 566, 750
SsiI CCGC 3 cut(s) 5, 54, 435
StyD4I CCNGG 1 cut(s) 72
TaaI ACNGT 2 cut(s) 355, 535
TaiI ACGT 3 cut(s) 26, 212, 499
TaqI TCGA 2 cut(s) 20, 26
TasI AATT 7 cut(s) 100, 168, 426, 438, 480, 566, 750
TfiI GAWTC 1 cut(s) 28
Tru1I TTAA 3 cut(s) 399, 468, 624
Tru9I TTAA 3 cut(s) 399, 468, 624
TscAI CASTG 2 cut(s) 39, 358
TseFI GTSAC 2 cut(s) 488, 608
TseI GCWGC 1 cut(s) 259
Tsp45I GTSAC 2 cut(s) 488, 608
TspDTI ATGAA 2 cut(s) 193, 576
TspGWI ACGGA 1 cut(s) 476
TspRI CASTG 2 cut(s) 39, 358
Van91I CCANNNNNTGG 1 cut(s) 500
VpaK11BI GGWCC 1 cut(s) 56
XapI RAATTY 1 cut(s) 750
XcmI CCANNNNNNNNNTGG 1 cut(s) 230
XmiI GTMKAC 1 cut(s) 20
ZraI GACGTC 1 cut(s) 24
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.