MD15G1339500.v1.1

Protein of unknown function (DUF502)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0002755
Physical Location & Seq
Forward (+)
38368177 .. 38375026
6850 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1339500.v1.1.491

Sequence Viewer

Length: 774 bp
ATGGCCGAAGATCAAGAATCGACGTCGATTCCTCTGAGTCAAGCGGAGAATGGTGGTCCGGATCCCGAAGATCCAGCCAAGTCTCCCCCTCCCTCCCCCAATTCCTCTACTCGTAAGGCTTGCTGCTTTGTTCTCCAAAGTTGGGTCTCAAAGAAGTTTATGACTGGATGTGTGGTGCTTTTTCCTGTTGCTGTTACATTCTTTGTTACATGGTGGTTTATTCAGTTTGTTGATGGTTTCTTTAGTCCACTCTATGCTCAGCTTGGCATCAACATCTTCGGACTTGGATTTGTCACATCCTTATTGTTTGTATTCTTTGTTGGCGTCTTTGTTTCATCATGGATGGGTGCCACTGTTTTCTCAGTCGGAGAATGGATCATAAAGAGAATGCCTTTTGTTAAGCATATTTACTCCGCCTCCAAACAAATTAGTGCTGCAGTTTCTCCAGACCAAAATACTACAGCTTTTAAAGAGGTGGCAATTATCCGTCACCCACGTGTTGGTGAATATGCTTTTGGATTTATTACATCAACAGTCACCCTTCAGAAAGATAATGAAGATGAAGAGTTGTGTAGTGTGTTTGTCCCAACAAATCATCTATACATAGGCGATATATTTCTTGTTAACTCCACAGAGATCATAAGACCGAATTTGTCTATCCGAGAAGGCATAGAGATCATTGTGTCTGGGGGTATGACAATGCCCCAAATCATTTCTCCTCAACAAAGGATTGCCCAGCAGAACGAGAAGATCCCTTTGAACAGAATAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF502

Protein Analysis

258

Amino Acids

28.59

Weight (kDa)

5.66

Isoelectric Point (pI)

48.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF502 PF04367 99 - 199 9e-28 Protein of unknown function (DUF502)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 26
AccB1I GGYRCC 1 cut(s) 347
AccB7I CCANNNNNTGG 1 cut(s) 500
AccIII TCCGGA 1 cut(s) 58
AciI CCGC 2 cut(s) 44, 414
AclWI GGATC 5 cut(s) 56, 65, 69, 383, 745
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 649
AcuI CTGAAG 1 cut(s) 527
AcvI CACGTG 1 cut(s) 497
AcyI GRCGYC 2 cut(s) 23, 324
AfiI CCNNNNNNNGG 2 cut(s) 142, 500
AflIII ACRYGT 1 cut(s) 496
AgsI TTSAA 1 cut(s) 760
AjuI GAANNNNNNNTTGG 2 cut(s) 498, 530
AleI CACNNNNGTG 1 cut(s) 495
AluBI AGCT 2 cut(s) 262, 464
AluI AGCT 2 cut(s) 262, 464
Alw26I GTCTC 2 cut(s) 87, 151
AlwI GGATC 5 cut(s) 56, 65, 69, 383, 745
Aor13HI TCCGGA 1 cut(s) 58
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 2 cut(s) 123, 434
ApoI RAATTY 1 cut(s) 649
AspS9I GGNCC 1 cut(s) 56
AsuHPI GGTGA 3 cut(s) 482, 515, 529
AvaII GGWCC 1 cut(s) 56
BamHI GGATCC 1 cut(s) 61
BanI GGYRCC 1 cut(s) 347
BbrPI CACGTG 1 cut(s) 497
BbvI GCAGC 2 cut(s) 110, 421
BccI CCATC 2 cut(s) 227, 337
BcoDI GTCTC 2 cut(s) 87, 151
BfmI CTRYAG 2 cut(s) 435, 459
BisI GCNGC 2 cut(s) 124, 435
BlpI GCTNAGC 1 cut(s) 258
BlsI GCNGC 2 cut(s) 125, 436
Bme18I GGWCC 1 cut(s) 56
BmgT120I GGNCC 1 cut(s) 56
BmiI GGNNCC 2 cut(s) 63, 349
BmsI GCATC 1 cut(s) 276
BpmI CTGGAG 1 cut(s) 429
Bpu1102I GCTNAGC 1 cut(s) 258
BsaAI YACGTR 1 cut(s) 497
BsaHI GRCGYC 2 cut(s) 23, 324
BsaI GGTCTC 1 cut(s) 151
BsaWI WCCGGW 1 cut(s) 58
BsaXI ACNNNNNCTCC 2 cut(s) 401, 431
Bsc4I CCNNNNNNNGG 2 cut(s) 142, 500
Bse1I ACTGG 1 cut(s) 169
BseAI TCCGGA 1 cut(s) 58
BseGI GGATG 3 cut(s) 173, 296, 348
BseLI CCNNNNNNNGG 2 cut(s) 142, 500
BseMII CTCAG 3 cut(s) 26, 272, 375
BseNI ACTGG 1 cut(s) 169
BseRI GAGGAG 1 cut(s) 708
BseXI GCAGC 2 cut(s) 110, 421
BseYI CCCAGC 1 cut(s) 735
BshFI GGCC 1 cut(s) 5
BshNI GGYRCC 1 cut(s) 347
BsiSI CCGG 1 cut(s) 59
BslFI GGGAC 1 cut(s) 569
BslI CCNNNNNNNGG 2 cut(s) 142, 500
BsmAI GTCTC 2 cut(s) 87, 151
BsmFI GGGAC 1 cut(s) 569
BsmI GAATGC 1 cut(s) 393
BsnI GGCC 1 cut(s) 5
Bso31I GGTCTC 1 cut(s) 151
Bsp13I TCCGGA 1 cut(s) 58
Bsp143I GATC 7 cut(s) 10, 61, 70, 375, 636, 675, 750
Bsp1720I GCTNAGC 1 cut(s) 258
BspACI CCGC 2 cut(s) 44, 414
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 3 cut(s) 27, 271, 374
BspEI TCCGGA 1 cut(s) 58
BspLI GGNNCC 2 cut(s) 63, 349
BspMAI CTGCAG 1 cut(s) 439
BspPI GGATC 5 cut(s) 56, 65, 69, 383, 745
BspT107I GGYRCC 1 cut(s) 347
BspTNI GGTCTC 1 cut(s) 151
BsrI ACTGG 1 cut(s) 169
BssMI GATC 7 cut(s) 10, 61, 70, 375, 636, 675, 750
BssNI GRCGYC 2 cut(s) 23, 324
Bst4CI ACNGT 2 cut(s) 355, 535
Bst6I CTCTTC 1 cut(s) 558
BstACI GRCGYC 2 cut(s) 23, 324
BstBAI YACGTR 1 cut(s) 497
BstC8I GCNNGC 1 cut(s) 121
BstDEI CTNAG 3 cut(s) 35, 258, 361
BstF5I GGATG 3 cut(s) 173, 296, 348
BstKTI GATC 7 cut(s) 13, 64, 73, 378, 639, 678, 753
BstMAI GTCTC 2 cut(s) 87, 151
BstMBI GATC 7 cut(s) 10, 61, 70, 375, 636, 675, 750
BstSFI CTRYAG 2 cut(s) 435, 459
BstV1I GCAGC 2 cut(s) 110, 421
BstX2I RGATCY 3 cut(s) 61, 70, 750
BstYI RGATCY 3 cut(s) 61, 70, 750
BsuRI GGCC 1 cut(s) 5
BtsCI GGATG 3 cut(s) 173, 296, 348
BtsIMutI CAGTG 1 cut(s) 351
Cac8I GCNNGC 1 cut(s) 121
Cfr13I GGNCC 1 cut(s) 56
CseI GACGC 1 cut(s) 313
CviAII CATG 2 cut(s) 210, 339
CviJI RGCY 5 cut(s) 5, 77, 119, 262, 464
CviKI_1 RGCY 5 cut(s) 5, 77, 119, 262, 464
DdeI CTNAG 3 cut(s) 35, 258, 361
DpnI GATC 7 cut(s) 12, 63, 72, 377, 638, 677, 752
DpnII GATC 7 cut(s) 10, 61, 70, 375, 636, 675, 750
DraI TTTAAA 1 cut(s) 469
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 558
EarI CTCTTC 1 cut(s) 558
EciI GGCGGA 1 cut(s) 403
Eco31I GGTCTC 1 cut(s) 151
Eco47I GGWCC 1 cut(s) 56
Eco57I CTGAAG 1 cut(s) 527
Eco72I CACGTG 1 cut(s) 497
FaeI CATG 2 cut(s) 213, 342
FaqI GGGAC 1 cut(s) 569
FatI CATG 2 cut(s) 209, 338
Fnu4HI GCNGC 2 cut(s) 124, 435
FokI GGATG 3 cut(s) 180, 283, 355
Fsp4HI GCNGC 2 cut(s) 124, 435
GluI GCNGC 2 cut(s) 124, 435
GsaI CCCAGC 1 cut(s) 739
GsuI CTGGAG 1 cut(s) 429
HaeIII GGCC 1 cut(s) 5
HapII CCGG 1 cut(s) 59
HgaI GACGC 1 cut(s) 313
Hin1I GRCGYC 2 cut(s) 23, 324
Hin1II CATG 2 cut(s) 213, 342
HincII GTYRAC 1 cut(s) 625
HindII GTYRAC 1 cut(s) 625
HinfI GANTC 3 cut(s) 17, 28, 37
HpaI GTTAAC 1 cut(s) 625
HpaII CCGG 1 cut(s) 59
HphI GGTGA 3 cut(s) 482, 515, 529
Hpy166II GTNNAC 2 cut(s) 248, 625
Hpy188I TCNGA 5 cut(s) 36, 281, 368, 546, 662
Hpy188III TCNNGA 4 cut(s) 14, 59, 65, 446
Hpy8I GTNNAC 2 cut(s) 248, 625
Hpy99I CGWCG 2 cut(s) 25, 28
HpyAV CCTTC 2 cut(s) 551, 659
HpyCH4III ACNGT 2 cut(s) 355, 535
HpyCH4IV ACGT 2 cut(s) 23, 496
HpyCH4V TGCA 1 cut(s) 437
HpyF3I CTNAG 3 cut(s) 35, 258, 361
HpySE526I ACGT 2 cut(s) 23, 496
Hsp92I GRCGYC 2 cut(s) 23, 324
Hsp92II CATG 2 cut(s) 213, 342
Kpn2I TCCGGA 1 cut(s) 58
KspAI GTTAAC 1 cut(s) 625
Kzo9I GATC 7 cut(s) 10, 61, 70, 375, 636, 675, 750
LpnPI CCDG 7 cut(s) 72, 87, 150, 198, 459, 672, 749
Lsp1109I GCAGC 2 cut(s) 110, 421
LweI GCATC 1 cut(s) 276
MaeII ACGT 2 cut(s) 23, 496
MaeIII GTNAC 5 cut(s) 193, 205, 292, 488, 535
MalI GATC 7 cut(s) 12, 63, 72, 377, 638, 677, 752
MboI GATC 7 cut(s) 10, 61, 70, 375, 636, 675, 750
MboII GAAGA 6 cut(s) 20, 80, 268, 569, 575, 760
MflI RGATCY 3 cut(s) 61, 70, 750
MluCI AATT 4 cut(s) 100, 426, 480, 649
MlyI GAGTC 1 cut(s) 46
MmeI TCCRAC 1 cut(s) 346
MnlI CCTC 7 cut(s) 42, 99, 103, 115, 427, 466, 729
MroI TCCGGA 1 cut(s) 58
MseI TTAA 3 cut(s) 399, 468, 624
MslI CAYNNNNRTG 1 cut(s) 495
MspI CCGG 1 cut(s) 59
Mva1269I GAATGC 1 cut(s) 393
NdeII GATC 7 cut(s) 10, 61, 70, 375, 636, 675, 750
NlaIII CATG 2 cut(s) 213, 342
NlaIV GGNNCC 2 cut(s) 63, 349
NmuCI GTSAC 3 cut(s) 292, 488, 535
OliI CACNNNNGTG 1 cut(s) 495
PctI GAATGC 1 cut(s) 393
PfeI GAWTC 2 cut(s) 17, 28
PflMI CCANNNNNTGG 1 cut(s) 500
PkrI GCNGC 2 cut(s) 125, 436
PleI GAGTC 1 cut(s) 45
PmaCI CACGTG 1 cut(s) 497
PmlI CACGTG 1 cut(s) 497
PpsI GAGTC 1 cut(s) 45
Ppu21I YACGTR 1 cut(s) 497
PspCI CACGTG 1 cut(s) 497
PspFI CCCAGC 1 cut(s) 735
PspN4I GGNNCC 2 cut(s) 63, 349
PspPI GGNCC 1 cut(s) 56
PstI CTGCAG 1 cut(s) 439
PsuI RGATCY 3 cut(s) 61, 70, 750
RseI CAYNNNNRTG 1 cut(s) 495
SaqAI TTAA 3 cut(s) 399, 468, 624
SatI GCNGC 2 cut(s) 124, 435
Sau3AI GATC 7 cut(s) 10, 61, 70, 375, 636, 675, 750
Sau96I GGNCC 1 cut(s) 56
SchI GAGTC 1 cut(s) 46
SetI ASST 5 cut(s) 26, 264, 466, 477, 499
SfaNI GCATC 1 cut(s) 276
SfcI CTRYAG 2 cut(s) 435, 459
SinI GGWCC 1 cut(s) 56
SmiMI CAYNNNNRTG 1 cut(s) 495
Sse9I AATT 4 cut(s) 100, 426, 480, 649
SsiI CCGC 2 cut(s) 44, 414
TaaI ACNGT 2 cut(s) 355, 535
TaiI ACGT 2 cut(s) 26, 499
TaqI TCGA 2 cut(s) 20, 26
TaqII GACCGA 1 cut(s) 661
TasI AATT 4 cut(s) 100, 426, 480, 649
TfiI GAWTC 2 cut(s) 17, 28
Tru1I TTAA 3 cut(s) 399, 468, 624
Tru9I TTAA 3 cut(s) 399, 468, 624
TscAI CASTG 1 cut(s) 358
TseFI GTSAC 3 cut(s) 292, 488, 535
TseI GCWGC 2 cut(s) 123, 434
Tsp45I GTSAC 3 cut(s) 292, 488, 535
TspDTI ATGAA 3 cut(s) 324, 570, 576
TspGWI ACGGA 1 cut(s) 476
TspRI CASTG 1 cut(s) 358
Van91I CCANNNNNTGG 1 cut(s) 500
VpaK11BI GGWCC 1 cut(s) 56
XapI RAATTY 1 cut(s) 649
ZraI GACGTC 1 cut(s) 24
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.