MD05G1030200.v1.1

Protein of unknown function (DUF502)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0002755
Physical Location & Seq
Reverse (-)
4871120 .. 4878447
7328 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1030200.v1.1.491

Sequence Viewer

Length: 768 bp
ATGGCGGAAGCTAAAGAGCCTACGTCGTCGAGTCCGCTGACCCAAACCGACCCTGAAGATGTTTCCAGGTCTCCGCCAAGCTCCCCCAACTCCTCAACTCGCAAGGCATGCTATGCAGTGCTTCAGAGTTGGGTCTCAAAGAAATTCATGACCGGATGTATGGTTCTCTTTCCTGTCGCAGTTACATTCTTCATCACTTGGTGGTTTGTTCAGTTTGTAGATGGTTTCTTCAGTCCAATATATGCCAGGCTCGGGGTTAACATATTTGGCCTAGGATTTGTTACATCCCTACTTTTTATAGTCTTGATTGGTATTTTTGCCTCATCATGGATGGGTGCAACAGTTTTCTGGATTGGAGAATGGTTCATTAAACGAATGCCCTTTATGAAGCACATATACTCGGCCTCTAAGCAGATTAGTTCTGCAATCTCTCCAGATCAAAATACCACTGCTTTTAAAGAGGTTGCAATTATTCGTCATCCTCGAGTTGGTGAATATGCATTTGGTTTTATCACATCATCTGTAACACTTCAGAGGGATGATGGAGATGAGGAGTTGTGTAGTGTTTATGTCCCAACAAACCATCTATACATTGGCGATATTTTTCTTGTCAATTCTGAAGAGATCATACGACCAAATTTGTCAGTTCGGGAAGGCATAGAGATCATAATCTCAGTTGGTATGACGATGCCGCAAGTGATTTCTCCAATCGAAAGGATTCCTCGTCAAAGCGACAGGATTCCACTGAACAGAATGGTGTCCCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF502

Protein Analysis

256

Amino Acids

28.64

Weight (kDa)

6.31

Isoelectric Point (pI)

58.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF502 PF04367 95 - 195 1e-26 Protein of unknown function (DUF502)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 5, 35, 74, 692
AcsI RAATTY 2 cut(s) 143, 637
AcuI CTGAAG 5 cut(s) 75, 107, 214, 515, 639
AdeI CACNNNGTG 1 cut(s) 201
AfiI CCNNNNNNNGG 3 cut(s) 252, 327, 488
AjnI CCWGG 2 cut(s) 65, 245
AjuI GAANNNNNNNTTGG 2 cut(s) 486, 518
AloI GAACNNNNNNTCC 2 cut(s) 147, 179
AluBI AGCT 2 cut(s) 11, 81
AluI AGCT 2 cut(s) 11, 81
Alw26I GTCTC 2 cut(s) 75, 139
Ama87I CYCGRG 2 cut(s) 251, 483
AoxI GGCC 2 cut(s) 268, 402
ApoI RAATTY 2 cut(s) 143, 637
Asp700I GAANNNNTTC 1 cut(s) 717
AspA2I CCTAGG 1 cut(s) 271
AsuHPI GGTGA 1 cut(s) 503
AvaI CYCGRG 2 cut(s) 251, 483
AvrII CCTAGG 1 cut(s) 271
BarI GAAGNNNNNNTAC 4 cut(s) 380, 412, 612, 644
BccI CCATC 4 cut(s) 215, 325, 536, 591
BciT130I CCWGG 2 cut(s) 67, 247
BcoDI GTCTC 2 cut(s) 75, 139
BfaI CTAG 1 cut(s) 272
BisI GCNGC 1 cut(s) 692
BlnI CCTAGG 1 cut(s) 271
BlsI GCNGC 1 cut(s) 693
Bme1390I CCNGG 2 cut(s) 67, 247
BmeT110I CYCGRG 2 cut(s) 251, 483
BmrFI CCNGG 2 cut(s) 67, 247
BmsI GCATC 1 cut(s) 678
BpmI CTGGAG 1 cut(s) 417
BsaBI GATNNNNATC 1 cut(s) 668
BsaI GGTCTC 2 cut(s) 75, 139
BsaJI CCNNGG 1 cut(s) 271
BsaWI WCCGGW 1 cut(s) 152
Bsc4I CCNNNNNNNGG 3 cut(s) 252, 327, 488
Bse8I GATNNNNATC 1 cut(s) 668
BseBI CCWGG 2 cut(s) 67, 247
BseDI CCNNGG 1 cut(s) 271
BseGI GGATG 5 cut(s) 161, 284, 336, 478, 544
BseJI GATNNNNATC 1 cut(s) 668
BseLI CCNNNNNNNGG 3 cut(s) 252, 327, 488
BseMII CTCAG 1 cut(s) 687
BseRI GAGGAG 2 cut(s) 82, 566
BshFI GGCC 2 cut(s) 270, 404
BsiHKCI CYCGRG 2 cut(s) 251, 483
BsiSI CCGG 1 cut(s) 153
BslFI GGGAC 2 cut(s) 557, 745
BslI CCNNNNNNNGG 3 cut(s) 252, 327, 488
BsmAI GTCTC 2 cut(s) 75, 139
BsmFI GGGAC 2 cut(s) 557, 745
BsmI GAATGC 1 cut(s) 381
BsnI GGCC 2 cut(s) 270, 404
Bso31I GGTCTC 2 cut(s) 75, 139
BsoBI CYCGRG 2 cut(s) 251, 483
Bsp143I GATC 3 cut(s) 436, 624, 663
BspACI CCGC 4 cut(s) 5, 35, 74, 692
BspANI GGCC 2 cut(s) 270, 404
BspCNI CTCAG 1 cut(s) 686
BspHI TCATGA 1 cut(s) 147
BspTNI GGTCTC 2 cut(s) 75, 139
BssECI CCNNGG 1 cut(s) 271
BssMI GATC 3 cut(s) 436, 624, 663
BssT1I CCWWGG 1 cut(s) 271
Bst2UI CCWGG 2 cut(s) 67, 247
Bst4CI ACNGT 1 cut(s) 343
Bst6I CTCTTC 1 cut(s) 615
BstAPI GCANNNNNTGC 2 cut(s) 108, 113
BstC8I GCNNGC 1 cut(s) 109
BstDEI CTNAG 2 cut(s) 408, 673
BstF5I GGATG 5 cut(s) 161, 284, 336, 478, 544
BstKTI GATC 3 cut(s) 439, 627, 666
BstMAI GTCTC 2 cut(s) 75, 139
BstMBI GATC 3 cut(s) 436, 624, 663
BstMWI GCNNNNNNNGC 2 cut(s) 108, 113
BstNI CCWGG 2 cut(s) 67, 247
BstNSI RCATGY 1 cut(s) 111
BstSCI CCNGG 2 cut(s) 65, 245
BsuRI GGCC 2 cut(s) 270, 404
BtsCI GGATG 5 cut(s) 161, 284, 336, 478, 544
BtsI GCAGTG 2 cut(s) 123, 447
BtsIMutI CAGTG 3 cut(s) 123, 447, 743
Cac8I GCNNGC 1 cut(s) 109
CciI TCATGA 1 cut(s) 147
CviAII CATG 3 cut(s) 108, 148, 327
CviJI RGCY 6 cut(s) 11, 19, 81, 250, 270, 404
CviKI_1 RGCY 6 cut(s) 11, 19, 81, 250, 270, 404
DdeI CTNAG 2 cut(s) 408, 673
DpnI GATC 3 cut(s) 438, 626, 665
DpnII GATC 3 cut(s) 436, 624, 663
DraI TTTAAA 1 cut(s) 457
DraIII CACNNNGTG 1 cut(s) 201
Eam1104I CTCTTC 1 cut(s) 615
EarI CTCTTC 1 cut(s) 615
EciI GGCGGA 2 cut(s) 20, 63
Eco130I CCWWGG 1 cut(s) 271
Eco31I GGTCTC 2 cut(s) 75, 139
Eco57I CTGAAG 5 cut(s) 75, 107, 214, 515, 639
Eco88I CYCGRG 2 cut(s) 251, 483
EcoRII CCWGG 2 cut(s) 65, 245
EcoT14I CCWWGG 1 cut(s) 271
EcoT22I ATGCAT 1 cut(s) 502
ErhI CCWWGG 1 cut(s) 271
FaeI CATG 3 cut(s) 111, 151, 330
FaqI GGGAC 2 cut(s) 557, 745
FatI CATG 3 cut(s) 107, 147, 326
Fnu4HI GCNGC 1 cut(s) 692
FokI GGATG 5 cut(s) 168, 271, 343, 465, 551
Fsp4HI GCNGC 1 cut(s) 692
FspBI CTAG 1 cut(s) 272
GluI GCNGC 1 cut(s) 692
GsuI CTGGAG 1 cut(s) 417
HaeIII GGCC 2 cut(s) 270, 404
HapII CCGG 1 cut(s) 153
Hin1II CATG 3 cut(s) 111, 151, 330
HincII GTYRAC 1 cut(s) 259
HindII GTYRAC 1 cut(s) 259
HinfI GANTC 3 cut(s) 31, 718, 739
HpaI GTTAAC 1 cut(s) 259
HpaII CCGG 1 cut(s) 153
HphI GGTGA 1 cut(s) 503
Hpy166II GTNNAC 1 cut(s) 259
Hpy188I TCNGA 3 cut(s) 126, 534, 619
Hpy188III TCNNGA 5 cut(s) 148, 304, 349, 434, 650
Hpy8I GTNNAC 1 cut(s) 259
Hpy99I CGWCG 2 cut(s) 28, 31
HpyAV CCTTC 1 cut(s) 647
HpyCH4III ACNGT 1 cut(s) 343
HpyCH4IV ACGT 1 cut(s) 23
HpyCH4V TGCA 5 cut(s) 116, 338, 425, 467, 500
HpyF10VI GCNNNNNNNGC 2 cut(s) 108, 113
HpyF3I CTNAG 2 cut(s) 408, 673
HpySE526I ACGT 1 cut(s) 23
Hsp92II CATG 3 cut(s) 111, 151, 330
KspAI GTTAAC 1 cut(s) 259
Kzo9I GATC 3 cut(s) 436, 624, 663
LmnI GCTCC 1 cut(s) 86
LweI GCATC 1 cut(s) 678
MaeI CTAG 1 cut(s) 272
MaeII ACGT 1 cut(s) 23
MaeIII GTNAC 3 cut(s) 181, 280, 523
MalI GATC 3 cut(s) 438, 626, 665
MboI GATC 3 cut(s) 436, 624, 663
MboII GAAGA 4 cut(s) 68, 181, 220, 632
MluCI AATT 4 cut(s) 143, 468, 613, 637
MlyI GAGTC 1 cut(s) 40
MnlI CCTC 8 cut(s) 103, 331, 415, 454, 492, 528, 544, 732
Mph1103I ATGCAT 1 cut(s) 502
MroXI GAANNNNTTC 1 cut(s) 717
MseI TTAA 3 cut(s) 258, 369, 456
MspA1I CMGCKG 1 cut(s) 37
MspI CCGG 1 cut(s) 153
MspR9I CCNGG 2 cut(s) 67, 247
Mva1269I GAATGC 1 cut(s) 381
MvaI CCWGG 2 cut(s) 67, 247
MwoI GCNNNNNNNGC 2 cut(s) 108, 113
NdeII GATC 3 cut(s) 436, 624, 663
NlaIII CATG 3 cut(s) 111, 151, 330
NmeAIII GCCGAG 1 cut(s) 380
NsiI ATGCAT 1 cut(s) 502
NspI RCATGY 1 cut(s) 111
PaeI GCATGC 1 cut(s) 111
PaeR7I CTCGAG 1 cut(s) 483
PagI TCATGA 1 cut(s) 147
PcsI WCGNNNNNNNCGW 1 cut(s) 481
PctI GAATGC 1 cut(s) 381
PdmI GAANNNNTTC 1 cut(s) 717
PfeI GAWTC 2 cut(s) 718, 739
PkrI GCNGC 1 cut(s) 693
PleI GAGTC 1 cut(s) 39
PpsI GAGTC 1 cut(s) 39
Psp6I CCWGG 2 cut(s) 65, 245
PspGI CCWGG 2 cut(s) 65, 245
PspXI VCTCGAGB 1 cut(s) 483
SaqAI TTAA 3 cut(s) 258, 369, 456
SatI GCNGC 1 cut(s) 692
Sau3AI GATC 3 cut(s) 436, 624, 663
SchI GAGTC 1 cut(s) 40
ScrFI CCNGG 2 cut(s) 67, 247
SetI ASST 5 cut(s) 13, 26, 71, 83, 465
SfaNI GCATC 1 cut(s) 678
Sfr274I CTCGAG 1 cut(s) 483
SlaI CTCGAG 1 cut(s) 483
SmlI CTYRAG 1 cut(s) 483
SmoI CTYRAG 1 cut(s) 483
SphI GCATGC 1 cut(s) 111
Sse9I AATT 4 cut(s) 143, 468, 613, 637
SsiI CCGC 4 cut(s) 5, 35, 74, 692
SspMI CTAG 1 cut(s) 272
StyD4I CCNGG 2 cut(s) 65, 245
StyI CCWWGG 1 cut(s) 271
TaaI ACNGT 1 cut(s) 343
TaiI ACGT 1 cut(s) 26
TaqI TCGA 3 cut(s) 29, 484, 711
TasI AATT 4 cut(s) 143, 468, 613, 637
TauI GCSGC 1 cut(s) 694
TfiI GAWTC 2 cut(s) 718, 739
Tru1I TTAA 3 cut(s) 258, 369, 456
Tru9I TTAA 3 cut(s) 258, 369, 456
TscAI CASTG 3 cut(s) 123, 454, 750
TspDTI ATGAA 4 cut(s) 136, 181, 355, 401
TspRI CASTG 3 cut(s) 123, 454, 750
XapI RAATTY 2 cut(s) 143, 637
XceI RCATGY 1 cut(s) 111
XcmI CCANNNNNNNNNTGG 1 cut(s) 590
XhoI CTCGAG 1 cut(s) 483
XmaJI CCTAGG 1 cut(s) 271
XmnI GAANNNNTTC 1 cut(s) 717
XspI CTAG 1 cut(s) 272
Zsp2I ATGCAT 1 cut(s) 502
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.