FvH4_2g20720
ERF Family

HAD superfamily, subfamily IIIB (Acid phosphatase)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0002586
Physical Location & Seq
Forward (+)
17313976 .. 17315233
1258 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g20720.t1

Sequence Viewer

Length: 768 bp
ATGGGAAAGCTCATAGAGTTCCTGCTTTTGATCTCAAGCCTCTTCACTGTGGTTGCAGCAGATTGGAGCATTTTGAACCAGAAGAAGAAGAGTAACAATGGGGCAGTGGGAGATGGCTTGAAGAAGTACTGTGAGAGTTGGAGGATCAATGTGGAGGTCAACAACATTAGAGGGTTTGATGTGGTGCCTCAAGAATGTGTTGAGCACATAAGGAATTACATGACATCTTCTCAGTACAACGCTGACTCAGAGAGGGCCTTGGAGGAAGTCACTCTCTACCTGAGCAGCAGTTACTGCTGCACTTTGGAGGGTGATGGTAAAGATGCTTGGATTTTTGATGTTGATGATACACATCTCCACTATTCCTTATTTCAAGAAACATGGGGAGAGAAGCTGAATGCAACATCTTTGGAAACGTGGATGAAAGAGAGCAAGGCACCAGCTCTAGAGCACACTTTAAAGCTCTTCCATGATATTAAAGACCAAGGATTTAAGATCTTTCTCATCTCTACAAGGAGGGAGACCCTTAGATCTTCAAGCGTAGATAACCTCATTAAGGTTGGATACCATGGATGGACTAGTCTTGTACTAAGGGGTCTCGAAGATGAATTTATGGAAGTGCAGATGTACAAGTCTAAGGCAAGGCATCGACTGATCGATGAAGGTTACCGCATTCGGGGAATCATCGGAGACCAGTGGAGCAGTTTCGAGGGCCTTCCTGCTGCTAAAAGAACATTCAAACTACCCAATTCCATGTACTATATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Acid_phosphat_B

Protein Analysis

256

Amino Acids

29.42

Weight (kDa)

6.01

Isoelectric Point (pI)

36.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Acid_phosphat_B PF03767 41 - 255 2.7e-66 HAD superfamily, subfamily IIIB (Acid phosphatase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 184, 436
AciI CCGC 1 cut(s) 670
AclWI GGATC 1 cut(s) 152
AcsI RAATTY 1 cut(s) 608
AfaI GTAC 5 cut(s) 128, 236, 588, 629, 758
AfiI CCNNNNNNNGG 2 cut(s) 556, 676
AgsI TTSAA 5 cut(s) 76, 121, 374, 537, 739
AhlI ACTAGT 1 cut(s) 578
AluBI AGCT 4 cut(s) 10, 394, 443, 463
AluI AGCT 4 cut(s) 10, 394, 443, 463
Alw21I GWGCWC 2 cut(s) 207, 453
Alw26I GTCTC 3 cut(s) 515, 602, 684
AlwI GGATC 1 cut(s) 152
AlwNI CAGNNNCTG 1 cut(s) 294
AoxI GGCC 2 cut(s) 255, 712
ApeKI GCWGC 4 cut(s) 56, 285, 297, 722
ApoI RAATTY 1 cut(s) 608
AspS9I GGNCC 2 cut(s) 255, 712
AsuHPI GGTGA 1 cut(s) 323
BanI GGYRCC 2 cut(s) 184, 436
Bbv12I GWGCWC 2 cut(s) 207, 453
BbvI GCAGC 4 cut(s) 68, 284, 297, 709
BccI CCATC 3 cut(s) 107, 308, 567
BciVI GTATCC 1 cut(s) 557
BcoDI GTCTC 3 cut(s) 515, 602, 684
BcuI ACTAGT 1 cut(s) 578
BfaI CTAG 3 cut(s) 446, 579, 766
BfuI GTATCC 1 cut(s) 557
BglII AGATCT 2 cut(s) 495, 530
BisI GCNGC 4 cut(s) 57, 286, 298, 723
BlsI GCNGC 4 cut(s) 58, 287, 299, 724
BmcAI AGTACT 1 cut(s) 128
BmgT120I GGNCC 2 cut(s) 255, 712
BmiI GGNNCC 2 cut(s) 186, 438
BmsI GCATC 2 cut(s) 313, 655
Bpu10I CCTNAGC 1 cut(s) 281
BpuEI CTTGAG 2 cut(s) 19, 174
Bsa29I ATCGAT 1 cut(s) 657
BsaBI GATNNNNATC 1 cut(s) 351
BsaI GGTCTC 3 cut(s) 515, 602, 684
BsaJI CCNNGG 3 cut(s) 258, 484, 568
Bsc4I CCNNNNNNNGG 2 cut(s) 556, 676
Bse1I ACTGG 1 cut(s) 694
Bse8I GATNNNNATC 1 cut(s) 351
BseCI ATCGAT 1 cut(s) 657
BseDI CCNNGG 3 cut(s) 258, 484, 568
BseGI GGATG 2 cut(s) 426, 578
BseJI GATNNNNATC 1 cut(s) 351
BseLI CCNNNNNNNGG 2 cut(s) 556, 676
BseMII CTCAG 3 cut(s) 245, 261, 272
BseNI ACTGG 1 cut(s) 694
BseXI GCAGC 4 cut(s) 68, 284, 297, 709
BsgI GTGCAG 2 cut(s) 283, 641
BshFI GGCC 2 cut(s) 257, 714
BshNI GGYRCC 2 cut(s) 184, 436
BshVI ATCGAT 1 cut(s) 657
BsiHKAI GWGCWC 2 cut(s) 207, 453
BslI CCNNNNNNNGG 2 cut(s) 556, 676
BsmAI GTCTC 3 cut(s) 515, 602, 684
BsmI GAATGC 2 cut(s) 403, 672
BsnI GGCC 2 cut(s) 257, 714
Bso31I GGTCTC 3 cut(s) 515, 602, 684
Bsp1286I GDGCHC 2 cut(s) 207, 453
Bsp1407I TGTACA 1 cut(s) 627
Bsp143I GATC 5 cut(s) 30, 144, 495, 530, 654
Bsp19I CCATGG 1 cut(s) 568
BspACI CCGC 1 cut(s) 670
BspANI GGCC 2 cut(s) 257, 714
BspCNI CTCAG 3 cut(s) 244, 260, 273
BspDI ATCGAT 1 cut(s) 657
BspLI GGNNCC 2 cut(s) 186, 438
BspPI GGATC 1 cut(s) 152
BspQI GCTCTTC 1 cut(s) 470
BspT107I GGYRCC 2 cut(s) 184, 436
BspTNI GGTCTC 3 cut(s) 515, 602, 684
BsrGI TGTACA 1 cut(s) 627
BsrI ACTGG 1 cut(s) 694
BssECI CCNNGG 3 cut(s) 258, 484, 568
BssMI GATC 5 cut(s) 30, 144, 495, 530, 654
BssT1I CCWWGG 3 cut(s) 258, 484, 568
Bst4CI ACNGT 2 cut(s) 49, 131
Bst6I CTCTTC 3 cut(s) 47, 83, 470
BstAPI GCANNNNNTGC 1 cut(s) 294
BstAUI TGTACA 1 cut(s) 627
BstDEI CTNAG 6 cut(s) 231, 247, 281, 527, 590, 636
BstDSI CCRYGG 1 cut(s) 568
BstEII GGTNACC 1 cut(s) 665
BstENI CCTNNNNNAGG 1 cut(s) 554
BstF5I GGATG 2 cut(s) 426, 578
BstKTI GATC 5 cut(s) 33, 147, 498, 533, 657
BstMAI GTCTC 3 cut(s) 515, 602, 684
BstMBI GATC 5 cut(s) 30, 144, 495, 530, 654
BstMWI GCNNNNNNNGC 1 cut(s) 294
BstPI GGTNACC 1 cut(s) 665
BstV1I GCAGC 4 cut(s) 68, 284, 297, 709
BstX2I RGATCY 2 cut(s) 495, 530
BstYI RGATCY 2 cut(s) 495, 530
Bsu15I ATCGAT 1 cut(s) 657
BsuI GTATCC 1 cut(s) 557
BsuRI GGCC 2 cut(s) 257, 714
BsuTUI ATCGAT 1 cut(s) 657
BtgI CCRYGG 1 cut(s) 568
BtsCI GGATG 2 cut(s) 426, 578
BtsI GCAGTG 1 cut(s) 111
BtsIMutI CAGTG 3 cut(s) 45, 111, 701
CaiI CAGNNNCTG 1 cut(s) 294
Cfr13I GGNCC 2 cut(s) 255, 712
ClaI ATCGAT 1 cut(s) 657
Csp6I GTAC 5 cut(s) 127, 235, 587, 628, 757
CviAII CATG 5 cut(s) 220, 381, 470, 569, 754
CviJI RGCY 8 cut(s) 10, 39, 117, 257, 394, 443, 463, 714
CviKI_1 RGCY 8 cut(s) 10, 39, 117, 257, 394, 443, 463, 714
CviQI GTAC 5 cut(s) 127, 235, 587, 628, 757
DdeI CTNAG 6 cut(s) 231, 247, 281, 527, 590, 636
DpnI GATC 5 cut(s) 32, 146, 497, 532, 656
DpnII GATC 5 cut(s) 30, 144, 495, 530, 654
DraI TTTAAA 1 cut(s) 459
Eam1104I CTCTTC 3 cut(s) 47, 83, 470
EarI CTCTTC 3 cut(s) 47, 83, 470
Eco130I CCWWGG 3 cut(s) 258, 484, 568
Eco31I GGTCTC 3 cut(s) 515, 602, 684
Eco91I GGTNACC 1 cut(s) 665
EcoNI CCTNNNNNAGG 1 cut(s) 554
EcoO109I RGGNCCY 2 cut(s) 255, 712
EcoO65I GGTNACC 1 cut(s) 665
EcoT14I CCWWGG 3 cut(s) 258, 484, 568
ErhI CCWWGG 3 cut(s) 258, 484, 568
FaeI CATG 5 cut(s) 223, 384, 473, 572, 757
FaiI YATR 9 cut(s) 14, 209, 221, 382, 471, 570, 614, 755, 762
FatI CATG 5 cut(s) 219, 380, 469, 568, 753
Fnu4HI GCNGC 4 cut(s) 57, 286, 298, 723
FokI GGATG 2 cut(s) 433, 585
Fsp4HI GCNGC 4 cut(s) 57, 286, 298, 723
FspBI CTAG 3 cut(s) 446, 579, 766
GluI GCNGC 4 cut(s) 57, 286, 298, 723
HaeIII GGCC 2 cut(s) 257, 714
Hin1II CATG 5 cut(s) 223, 384, 473, 572, 757
HincII GTYRAC 1 cut(s) 160
HindII GTYRAC 1 cut(s) 160
HinfI GANTC 2 cut(s) 245, 681
HphI GGTGA 1 cut(s) 323
Hpy166II GTNNAC 1 cut(s) 160
Hpy188I TCNGA 2 cut(s) 250, 689
Hpy188III TCNNGA 4 cut(s) 191, 374, 446, 599
Hpy8I GTNNAC 1 cut(s) 160
HpyAV CCTTC 2 cut(s) 656, 725
HpyCH4III ACNGT 2 cut(s) 49, 131
HpyCH4IV ACGT 1 cut(s) 416
HpyCH4V TGCA 4 cut(s) 56, 300, 401, 622
HpyF10VI GCNNNNNNNGC 1 cut(s) 294
HpyF3I CTNAG 6 cut(s) 231, 247, 281, 527, 590, 636
HpySE526I ACGT 1 cut(s) 416
Hsp92II CATG 5 cut(s) 223, 384, 473, 572, 757
Kzo9I GATC 5 cut(s) 30, 144, 495, 530, 654
LguI GCTCTTC 1 cut(s) 470
LmnI GCTCC 2 cut(s) 66, 699
LpnPI CCDG 6 cut(s) 35, 92, 293, 453, 707, 732
Lsp1109I GCAGC 4 cut(s) 68, 284, 297, 709
LweI GCATC 2 cut(s) 313, 655
MaeI CTAG 3 cut(s) 446, 579, 766
MaeII ACGT 1 cut(s) 416
MaeIII GTNAC 4 cut(s) 92, 268, 290, 665
MalI GATC 5 cut(s) 32, 146, 497, 532, 656
MboI GATC 5 cut(s) 30, 144, 495, 530, 654
MboII GAAGA 9 cut(s) 34, 94, 97, 100, 133, 219, 457, 525, 614
MflI RGATCY 2 cut(s) 495, 530
MhlI GDGCHC 2 cut(s) 207, 453
MluCI AATT 3 cut(s) 214, 608, 748
MlyI GAGTC 1 cut(s) 239
MmeI TCCRAC 2 cut(s) 119, 541
MseI TTAA 4 cut(s) 458, 477, 492, 555
Mva1269I GAATGC 2 cut(s) 403, 672
MwoI GCNNNNNNNGC 1 cut(s) 294
NcoI CCATGG 1 cut(s) 568
NdeII GATC 5 cut(s) 30, 144, 495, 530, 654
NlaIII CATG 5 cut(s) 223, 384, 473, 572, 757
NlaIV GGNNCC 2 cut(s) 186, 438
NmuCI GTSAC 1 cut(s) 268
PciSI GCTCTTC 1 cut(s) 470
PctI GAATGC 2 cut(s) 403, 672
PfeI GAWTC 1 cut(s) 681
PkrI GCNGC 4 cut(s) 58, 287, 299, 724
PleI GAGTC 1 cut(s) 239
PpsI GAGTC 1 cut(s) 239
PspEI GGTNACC 1 cut(s) 665
PspN4I GGNNCC 2 cut(s) 186, 438
PspPI GGNCC 2 cut(s) 255, 712
PstNI CAGNNNCTG 1 cut(s) 294
PsuI RGATCY 2 cut(s) 495, 530
RsaI GTAC 5 cut(s) 128, 236, 588, 629, 758
RsaNI GTAC 5 cut(s) 127, 235, 587, 628, 757
SapI GCTCTTC 1 cut(s) 470
SaqAI TTAA 4 cut(s) 458, 477, 492, 555
SatI GCNGC 4 cut(s) 57, 286, 298, 723
Sau3AI GATC 5 cut(s) 30, 144, 495, 530, 654
Sau96I GGNCC 2 cut(s) 255, 712
ScaI AGTACT 1 cut(s) 128
SchI GAGTC 1 cut(s) 239
SduI GDGCHC 2 cut(s) 207, 453
SfaNI GCATC 2 cut(s) 313, 655
SmlI CTYRAG 2 cut(s) 34, 189
SmoI CTYRAG 2 cut(s) 34, 189
SpeI ACTAGT 1 cut(s) 578
Sse9I AATT 3 cut(s) 214, 608, 748
SsiI CCGC 1 cut(s) 670
SspMI CTAG 3 cut(s) 446, 579, 766
StyI CCWWGG 3 cut(s) 258, 484, 568
TaaI ACNGT 2 cut(s) 49, 131
TaiI ACGT 1 cut(s) 419
TaqI TCGA 4 cut(s) 600, 649, 657, 708
TasI AATT 3 cut(s) 214, 608, 748
TatI WGTACW 5 cut(s) 126, 234, 586, 627, 756
TfiI GAWTC 1 cut(s) 681
Tru1I TTAA 4 cut(s) 458, 477, 492, 555
Tru9I TTAA 4 cut(s) 458, 477, 492, 555
TscAI CASTG 3 cut(s) 52, 111, 701
TseFI GTSAC 1 cut(s) 268
TseI GCWGC 4 cut(s) 56, 285, 297, 722
Tsp45I GTSAC 1 cut(s) 268
TspDTI ATGAA 3 cut(s) 437, 621, 675
TspRI CASTG 3 cut(s) 52, 111, 701
XagI CCTNNNNNAGG 1 cut(s) 554
XapI RAATTY 1 cut(s) 608
XbaI TCTAGA 1 cut(s) 445
XspI CTAG 3 cut(s) 446, 579, 766
ZrmI AGTACT 1 cut(s) 128
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.