Rroxscaffold_5G00340020

acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0002586
Physical Location & Seq
Reverse (-)
8392017 .. 8393222
1206 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00340020.1

Sequence Viewer

Length: 468 bp
ATGAAAGGTACAGGATCTGCAATTACTGGTCCTATTGGGAAGGAGTGTGCTGATCTGTGGCCTAGGATTGCTACTGCTGCTAATGCTATCGTTTCAGTGATACCATCTTCCGGCCATCTCTCGGCAGGAGACAGATCTTGTTTCTTTGTTACTCTAACAGTAGCAGAGTCGGAGTGGAACATTTTGAATCAGAGAACCAGGAATGGGATGAAGATCAGCTTGAAGACTTACTGTGAGAGCTGGAGGATGAATGTTGAGCTGCACAACATTAGAGAGTTCCAAGTGGTGCCTGAAGAGTGCATAGGATACGTTGGAAAATATGTTACTTCGACTCAGTACAAGGTGGACTCAGAAAGGGCTATTGAGGAGTCCATTGTGTATCTAAGCACTGGCTGTAATTTGAAGAATGATGGTAGAGATGCATGGATTTTTGACATTGATGATACACTGCTCTCTACTGTGCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Acid_phosphat_B

Protein Analysis

155

Amino Acids

17.18

Weight (kDa)

5.18

Isoelectric Point (pI)

36.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Acid_phosphat_B PF03767 72 - 155 5e-23 HAD superfamily, subfamily IIIB (Acid phosphatase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 286
AclWI GGATC 1 cut(s) 22
AcoI YGGCCR 1 cut(s) 112
AcuI CTGAAG 1 cut(s) 312
AfaI GTAC 2 cut(s) 10, 338
AfiI CCNNNNNNNGG 3 cut(s) 110, 121, 204
AgsI TTSAA 3 cut(s) 187, 223, 403
AjnI CCWGG 1 cut(s) 197
AluBI AGCT 3 cut(s) 219, 240, 259
AluI AGCT 3 cut(s) 219, 240, 259
Alw26I GTCTC 1 cut(s) 123
AlwI GGATC 1 cut(s) 22
AlwNI CAGNNNCTG 1 cut(s) 17
AoxI GGCC 2 cut(s) 59, 112
ApeKI GCWGC 2 cut(s) 77, 259
AspA2I CCTAGG 1 cut(s) 62
AspS9I GGNCC 1 cut(s) 29
AvaII GGWCC 1 cut(s) 29
AvrII CCTAGG 1 cut(s) 62
BanI GGYRCC 1 cut(s) 286
BbsI GAAGAC 1 cut(s) 230
BbvI GCAGC 2 cut(s) 64, 246
BccI CCATC 3 cut(s) 112, 123, 404
BciT130I CCWGG 1 cut(s) 199
BciVI GTATCC 1 cut(s) 299
BcoDI GTCTC 1 cut(s) 123
BfaI CTAG 1 cut(s) 63
BfuI GTATCC 1 cut(s) 299
BglII AGATCT 1 cut(s) 134
BisI GCNGC 2 cut(s) 78, 260
BlnI CCTAGG 1 cut(s) 62
BlsI GCNGC 2 cut(s) 79, 261
Bme1390I CCNGG 1 cut(s) 199
Bme18I GGWCC 1 cut(s) 29
BmgT120I GGNCC 1 cut(s) 29
BmiI GGNNCC 1 cut(s) 288
BmrFI CCNGG 1 cut(s) 199
BmsI GCATC 1 cut(s) 409
BpiI GAAGAC 1 cut(s) 230
BpmI CTGGAG 1 cut(s) 262
BsaBI GATNNNNATC 1 cut(s) 212
BsaJI CCNNGG 1 cut(s) 62
Bsc4I CCNNNNNNNGG 3 cut(s) 110, 121, 204
Bse1I ACTGG 2 cut(s) 31, 394
Bse8I GATNNNNATC 1 cut(s) 212
BseBI CCWGG 1 cut(s) 199
BseDI CCNNGG 1 cut(s) 62
BseGI GGATG 2 cut(s) 213, 252
BseJI GATNNNNATC 1 cut(s) 212
BseLI CCNNNNNNNGG 3 cut(s) 110, 121, 204
BseMII CTCAG 2 cut(s) 347, 363
BseNI ACTGG 2 cut(s) 31, 394
BseRI GAGGAG 1 cut(s) 380
BseXI GCAGC 2 cut(s) 64, 246
BsgI GTGCAG 1 cut(s) 245
BshFI GGCC 2 cut(s) 61, 114
BshNI GGYRCC 1 cut(s) 286
BsiSI CCGG 1 cut(s) 111
BslI CCNNNNNNNGG 3 cut(s) 110, 121, 204
BsmAI GTCTC 1 cut(s) 123
BsnI GGCC 2 cut(s) 61, 114
Bsp143I GATC 4 cut(s) 14, 52, 134, 213
BspANI GGCC 2 cut(s) 61, 114
BspCNI CTCAG 2 cut(s) 346, 362
BspLI GGNNCC 1 cut(s) 288
BspPI GGATC 1 cut(s) 22
BspT107I GGYRCC 1 cut(s) 286
BsrI ACTGG 2 cut(s) 31, 394
BssECI CCNNGG 1 cut(s) 62
BssMI GATC 4 cut(s) 14, 52, 134, 213
BssT1I CCWWGG 1 cut(s) 62
Bst2UI CCWGG 1 cut(s) 199
Bst4CI ACNGT 3 cut(s) 160, 233, 460
Bst6I CTCTTC 1 cut(s) 288
BstDEI CTNAG 3 cut(s) 333, 349, 383
BstF5I GGATG 2 cut(s) 213, 252
BstKTI GATC 4 cut(s) 17, 55, 137, 216
BstMAI GTCTC 1 cut(s) 123
BstMBI GATC 4 cut(s) 14, 52, 134, 213
BstMWI GCNNNNNNNGC 2 cut(s) 77, 83
BstNI CCWGG 1 cut(s) 199
BstSCI CCNGG 1 cut(s) 197
BstV1I GCAGC 2 cut(s) 64, 246
BstV2I GAAGAC 1 cut(s) 230
BstX2I RGATCY 2 cut(s) 14, 134
BstYI RGATCY 2 cut(s) 14, 134
BsuI GTATCC 1 cut(s) 299
BsuRI GGCC 2 cut(s) 61, 114
BtsCI GGATG 2 cut(s) 213, 252
BtsI GCAGTG 1 cut(s) 446
BtsIMutI CAGTG 3 cut(s) 102, 387, 446
CaiI CAGNNNCTG 1 cut(s) 17
Cfr13I GGNCC 1 cut(s) 29
Csp6I GTAC 2 cut(s) 9, 337
CviAII CATG 1 cut(s) 423
CviJI RGCY 7 cut(s) 61, 114, 219, 240, 259, 359, 393
CviKI_1 RGCY 7 cut(s) 61, 114, 219, 240, 259, 359, 393
CviQI GTAC 2 cut(s) 9, 337
DdeI CTNAG 3 cut(s) 333, 349, 383
DpnI GATC 4 cut(s) 16, 54, 136, 215
DpnII GATC 4 cut(s) 14, 52, 134, 213
EaeI YGGCCR 1 cut(s) 112
Eam1104I CTCTTC 1 cut(s) 288
EarI CTCTTC 1 cut(s) 288
Eco130I CCWWGG 1 cut(s) 62
Eco47I GGWCC 1 cut(s) 29
Eco57I CTGAAG 1 cut(s) 312
EcoRII CCWGG 1 cut(s) 197
EcoT14I CCWWGG 1 cut(s) 62
EcoT22I ATGCAT 1 cut(s) 424
ErhI CCWWGG 1 cut(s) 62
FaeI CATG 1 cut(s) 426
FaiI YATR 3 cut(s) 302, 321, 424
FalI AAGNNNNNCTT 2 cut(s) 203, 235
FatI CATG 1 cut(s) 422
Fnu4HI GCNGC 2 cut(s) 78, 260
FokI GGATG 2 cut(s) 220, 259
Fsp4HI GCNGC 2 cut(s) 78, 260
FspBI CTAG 1 cut(s) 63
GluI GCNGC 2 cut(s) 78, 260
GsuI CTGGAG 1 cut(s) 262
HaeIII GGCC 2 cut(s) 61, 114
HapII CCGG 1 cut(s) 111
Hin1II CATG 1 cut(s) 426
HinfI GANTC 5 cut(s) 167, 187, 331, 347, 368
HpaII CCGG 1 cut(s) 111
Hpy166II GTNNAC 1 cut(s) 346
Hpy188I TCNGA 3 cut(s) 172, 192, 352
Hpy8I GTNNAC 1 cut(s) 346
HpyAV CCTTC 1 cut(s) 34
HpyCH4III ACNGT 3 cut(s) 160, 233, 460
HpyCH4IV ACGT 1 cut(s) 309
HpyCH4V TGCA 4 cut(s) 20, 262, 300, 422
HpyF10VI GCNNNNNNNGC 2 cut(s) 77, 83
HpyF3I CTNAG 3 cut(s) 333, 349, 383
HpySE526I ACGT 1 cut(s) 309
Hsp92II CATG 1 cut(s) 426
Kzo9I GATC 4 cut(s) 14, 52, 134, 213
LpnPI CCDG 8 cut(s) 12, 111, 124, 184, 211, 226, 303, 375
Lsp1109I GCAGC 2 cut(s) 64, 246
LweI GCATC 1 cut(s) 409
MaeI CTAG 1 cut(s) 63
MaeII ACGT 1 cut(s) 309
MaeIII GTNAC 2 cut(s) 148, 322
MalI GATC 4 cut(s) 16, 54, 136, 215
MboI GATC 4 cut(s) 14, 52, 134, 213
MboII GAAGA 5 cut(s) 99, 223, 235, 305, 415
MflI RGATCY 2 cut(s) 14, 134
MluCI AATT 2 cut(s) 21, 397
MlyI GAGTC 4 cut(s) 176, 325, 341, 377
MmeI TCCRAC 2 cut(s) 150, 292
MnlI CCTC 2 cut(s) 237, 358
Mph1103I ATGCAT 1 cut(s) 424
MseI TTAA 1 cut(s) 466
MspI CCGG 1 cut(s) 111
MspR9I CCNGG 1 cut(s) 199
MvaI CCWGG 1 cut(s) 199
MwoI GCNNNNNNNGC 2 cut(s) 77, 83
NdeII GATC 4 cut(s) 14, 52, 134, 213
NlaIII CATG 1 cut(s) 426
NlaIV GGNNCC 1 cut(s) 288
NmeAIII GCCGAG 1 cut(s) 101
NsiI ATGCAT 1 cut(s) 424
PfeI GAWTC 1 cut(s) 187
PkrI GCNGC 2 cut(s) 79, 261
PleI GAGTC 4 cut(s) 175, 325, 341, 376
PpsI GAGTC 4 cut(s) 175, 325, 341, 376
Psp6I CCWGG 1 cut(s) 197
PspGI CCWGG 1 cut(s) 197
PspN4I GGNNCC 1 cut(s) 288
PspPI GGNCC 1 cut(s) 29
PstNI CAGNNNCTG 1 cut(s) 17
PsuI RGATCY 2 cut(s) 14, 134
RsaI GTAC 2 cut(s) 10, 338
RsaNI GTAC 2 cut(s) 9, 337
SaqAI TTAA 1 cut(s) 466
SatI GCNGC 2 cut(s) 78, 260
Sau3AI GATC 4 cut(s) 14, 52, 134, 213
Sau96I GGNCC 1 cut(s) 29
SchI GAGTC 4 cut(s) 176, 325, 341, 377
ScrFI CCNGG 1 cut(s) 199
SetI ASST 6 cut(s) 10, 221, 242, 261, 312, 345
SfaNI GCATC 1 cut(s) 409
SinI GGWCC 1 cut(s) 29
Sse9I AATT 2 cut(s) 21, 397
SspMI CTAG 1 cut(s) 63
StyD4I CCNGG 1 cut(s) 197
StyI CCWWGG 1 cut(s) 62
TaaI ACNGT 3 cut(s) 160, 233, 460
TaiI ACGT 1 cut(s) 312
TaqI TCGA 1 cut(s) 329
TasI AATT 2 cut(s) 21, 397
TatI WGTACW 1 cut(s) 336
TfiI GAWTC 1 cut(s) 187
Tru1I TTAA 1 cut(s) 466
Tru9I TTAA 1 cut(s) 466
TscAI CASTG 3 cut(s) 102, 394, 453
TseI GCWGC 2 cut(s) 77, 259
TspDTI ATGAA 3 cut(s) 17, 224, 263
TspRI CASTG 3 cut(s) 102, 394, 453
VpaK11BI GGWCC 1 cut(s) 29
XmaJI CCTAGG 1 cut(s) 62
XspI CTAG 1 cut(s) 63
Zsp2I ATGCAT 1 cut(s) 424
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.