Rw4G004570

acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0002586
Physical Location & Seq
Reverse (-)
9106843 .. 9107996
1154 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G004570.1

Sequence Viewer

Length: 504 bp
ATGGCCAGAAATCTATGCCACTTCTGTTTTTTGAGTTTCTTTGTTACTCTAACAGTAGCAGAGTCCGAGTGGAACATTTTGAATCAGAGAACCAGGAATGGGATGAAGATCAGCTTGAAGAATTACTGTGAGAGCTGGAGGATGAATGTAGAGCTGCACAACATTAGAGAGTTCCAAGTGGTGCCTGAAGAGTGCATAGGATACGTTGGAAAATATGTTACTTCGACTCAGTACAAGGTGGACTCAGAAAGGGCTATTGAGGAGTCCATTGTGTATCTAAGCACTAGCTGTAATTTGAAGAATGATGGTAGAGATGTATGGATTTTTGACATTGATGATACACTGCTCTCTACTAAGTTGAACCTGACTGCTTTGGAGGAGTGGATGAGCCATGGCAAGGCACCTGCTTTGGAACACTCGCTCAAACTCTGCAATGAGATTGAAAAGGGCTGCATATCATTTTGGTTTCTTCAAGGAGGGAGCATCTTAGATCAACCACAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Acid_phosphat_B

Protein Analysis

167

Amino Acids

19.33

Weight (kDa)

5.22

Isoelectric Point (pI)

45.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Acid_phosphat_B PF03767 37 - 154 2.6e-25 HAD superfamily, subfamily IIIB (Acid phosphatase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 412
Acc36I ACCTGC 1 cut(s) 412
AccB1I GGYRCC 2 cut(s) 181, 400
AcoI YGGCCR 1 cut(s) 3
AcuI CTGAAG 1 cut(s) 207
AfaI GTAC 1 cut(s) 233
AfiI CCNNNNNNNGG 2 cut(s) 99, 397
AgsI TTSAA 6 cut(s) 82, 118, 298, 361, 443, 473
AjnI CCWGG 1 cut(s) 92
AluBI AGCT 4 cut(s) 114, 135, 154, 288
AluI AGCT 4 cut(s) 114, 135, 154, 288
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 2 cut(s) 154, 450
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 2 cut(s) 181, 400
BbvI GCAGC 2 cut(s) 141, 437
BccI CCATC 1 cut(s) 299
BciT130I CCWGG 1 cut(s) 94
BciVI GTATCC 1 cut(s) 194
BfaI CTAG 1 cut(s) 285
BfuAI ACCTGC 1 cut(s) 412
BfuI GTATCC 1 cut(s) 194
BisI GCNGC 2 cut(s) 155, 451
BlsI GCNGC 2 cut(s) 156, 452
Bme1390I CCNGG 1 cut(s) 94
BmiI GGNNCC 2 cut(s) 183, 402
BmrFI CCNGG 1 cut(s) 94
BmsI GCATC 1 cut(s) 492
BpmI CTGGAG 1 cut(s) 157
BsaBI GATNNNNATC 1 cut(s) 107
BsaJI CCNNGG 1 cut(s) 391
Bsc4I CCNNNNNNNGG 2 cut(s) 99, 397
Bse3DI GCAATG 1 cut(s) 439
Bse8I GATNNNNATC 1 cut(s) 107
BseBI CCWGG 1 cut(s) 94
BseDI CCNNGG 1 cut(s) 391
BseGI GGATG 3 cut(s) 108, 147, 390
BseJI GATNNNNATC 1 cut(s) 107
BseLI CCNNNNNNNGG 2 cut(s) 99, 397
BseMI GCAATG 1 cut(s) 439
BseMII CTCAG 2 cut(s) 242, 258
BseRI GAGGAG 2 cut(s) 275, 392
BseXI GCAGC 2 cut(s) 141, 437
BsgI GTGCAG 1 cut(s) 140
BshFI GGCC 1 cut(s) 5
BshNI GGYRCC 2 cut(s) 181, 400
BslI CCNNNNNNNGG 2 cut(s) 99, 397
BsnI GGCC 1 cut(s) 5
Bsp143I GATC 2 cut(s) 108, 490
Bsp19I CCATGG 1 cut(s) 391
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 2 cut(s) 241, 257
BspLI GGNNCC 2 cut(s) 183, 402
BspMI ACCTGC 1 cut(s) 412
BspT107I GGYRCC 2 cut(s) 181, 400
BsrDI GCAATG 1 cut(s) 439
BssECI CCNNGG 1 cut(s) 391
BssMI GATC 2 cut(s) 108, 490
BssT1I CCWWGG 1 cut(s) 391
Bst2UI CCWGG 1 cut(s) 94
Bst4CI ACNGT 2 cut(s) 55, 128
Bst6I CTCTTC 1 cut(s) 183
BstDEI CTNAG 5 cut(s) 228, 244, 278, 354, 487
BstDSI CCRYGG 1 cut(s) 391
BstF5I GGATG 3 cut(s) 108, 147, 390
BstKTI GATC 2 cut(s) 111, 493
BstMBI GATC 2 cut(s) 108, 490
BstNI CCWGG 1 cut(s) 94
BstSCI CCNGG 1 cut(s) 92
BstV1I GCAGC 2 cut(s) 141, 437
BsuI GTATCC 1 cut(s) 194
BsuRI GGCC 1 cut(s) 5
BtgI CCRYGG 1 cut(s) 391
BtsCI GGATG 3 cut(s) 108, 147, 390
BtsI GCAGTG 1 cut(s) 341
BtsIMutI CAGTG 1 cut(s) 341
BveI ACCTGC 1 cut(s) 412
Csp6I GTAC 1 cut(s) 232
CviAII CATG 1 cut(s) 392
CviJI RGCY 8 cut(s) 5, 114, 135, 154, 254, 288, 390, 450
CviKI_1 RGCY 8 cut(s) 5, 114, 135, 154, 254, 288, 390, 450
CviQI GTAC 1 cut(s) 232
DdeI CTNAG 5 cut(s) 228, 244, 278, 354, 487
DpnI GATC 2 cut(s) 110, 492
DpnII GATC 2 cut(s) 108, 490
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 183
EarI CTCTTC 1 cut(s) 183
Eco130I CCWWGG 1 cut(s) 391
Eco57I CTGAAG 1 cut(s) 207
EcoRII CCWGG 1 cut(s) 92
EcoT14I CCWWGG 1 cut(s) 391
ErhI CCWWGG 1 cut(s) 391
FaeI CATG 1 cut(s) 395
FaiI YATR 6 cut(s) 16, 197, 216, 319, 393, 455
FalI AAGNNNNNCTT 2 cut(s) 98, 130
FatI CATG 1 cut(s) 391
Fnu4HI GCNGC 2 cut(s) 155, 451
FokI GGATG 3 cut(s) 115, 154, 397
Fsp4HI GCNGC 2 cut(s) 155, 451
FspBI CTAG 1 cut(s) 285
GluI GCNGC 2 cut(s) 155, 451
GsuI CTGGAG 1 cut(s) 157
HaeIII GGCC 1 cut(s) 5
Hin1II CATG 1 cut(s) 395
HinfI GANTC 5 cut(s) 62, 82, 226, 242, 263
Hpy166II GTNNAC 1 cut(s) 241
Hpy188I TCNGA 3 cut(s) 67, 87, 247
Hpy8I GTNNAC 1 cut(s) 241
HpyCH4III ACNGT 2 cut(s) 55, 128
HpyCH4IV ACGT 1 cut(s) 204
HpyCH4V TGCA 4 cut(s) 157, 195, 432, 453
HpyF3I CTNAG 5 cut(s) 228, 244, 278, 354, 487
HpySE526I ACGT 1 cut(s) 204
Hsp92II CATG 1 cut(s) 395
Kzo9I GATC 2 cut(s) 108, 490
LmnI GCTCC 1 cut(s) 480
LpnPI CCDG 7 cut(s) 19, 79, 106, 121, 198, 377, 417
Lsp1109I GCAGC 2 cut(s) 141, 437
LweI GCATC 1 cut(s) 492
MaeI CTAG 1 cut(s) 285
MaeII ACGT 1 cut(s) 204
MaeIII GTNAC 2 cut(s) 43, 217
MalI GATC 2 cut(s) 110, 492
MboI GATC 2 cut(s) 108, 490
MboII GAAGA 5 cut(s) 118, 130, 200, 310, 461
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 2 cut(s) 121, 292
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 4 cut(s) 71, 220, 236, 272
MmeI TCCRAC 1 cut(s) 187
MnlI CCTC 4 cut(s) 132, 253, 370, 470
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
Msp20I TGGCCA 1 cut(s) 5
MspR9I CCNGG 1 cut(s) 94
MvaI CCWGG 1 cut(s) 94
NcoI CCATGG 1 cut(s) 391
NdeII GATC 2 cut(s) 108, 490
NlaIII CATG 1 cut(s) 395
NlaIV GGNNCC 2 cut(s) 183, 402
PaqCI CACCTGC 1 cut(s) 412
PfeI GAWTC 1 cut(s) 82
PkrI GCNGC 2 cut(s) 156, 452
PleI GAGTC 4 cut(s) 70, 220, 236, 271
PpsI GAGTC 4 cut(s) 70, 220, 236, 271
Psp6I CCWGG 1 cut(s) 92
PspGI CCWGG 1 cut(s) 92
PspN4I GGNNCC 2 cut(s) 183, 402
RsaI GTAC 1 cut(s) 233
RsaNI GTAC 1 cut(s) 232
SatI GCNGC 2 cut(s) 155, 451
Sau3AI GATC 2 cut(s) 108, 490
SchI GAGTC 4 cut(s) 71, 220, 236, 272
ScrFI CCNGG 1 cut(s) 94
SetI ASST 8 cut(s) 116, 137, 156, 207, 240, 290, 366, 406
SfaNI GCATC 1 cut(s) 492
Sse9I AATT 2 cut(s) 121, 292
SspMI CTAG 1 cut(s) 285
StyD4I CCNGG 1 cut(s) 92
StyI CCWWGG 1 cut(s) 391
TaaI ACNGT 2 cut(s) 55, 128
TaiI ACGT 1 cut(s) 207
TaqI TCGA 1 cut(s) 224
TasI AATT 2 cut(s) 121, 292
TatI WGTACW 1 cut(s) 231
TfiI GAWTC 1 cut(s) 82
TscAI CASTG 1 cut(s) 348
TseI GCWGC 2 cut(s) 154, 450
TspDTI ATGAA 2 cut(s) 119, 158
TspRI CASTG 1 cut(s) 348
XspI CTAG 1 cut(s) 285
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.