Rh6AG296600
ERF Family

HAD superfamily, subfamily IIIB (Acid phosphatase)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0002586
Physical Location & Seq
Forward (+)
49443361 .. 49446307
2947 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG296600.1

Sequence Viewer

Length: 957 bp
ATGATTTCCCAGAGAACATTGAAGGCGCAAGGTTTTACTTCTAGATGTATAGATATTTTCCAGTTTACTTTAGTCTGTATAGTCATGCAACTTAAATTTTCCTGGGGATTGTTGGCTGTTAGTAGCATGCCAAATTTATTTTCACTGTCGACCTATTCTTGTCTCTGCAATGGCCTTTTTAATGGTCATTTTGTTGATAAGGAGGAGCAAACTGAACTTGAAAGTTCTGTTTATAATGCAGATTGGAGCATTTTGAACCGGAGAAAGAAGAATACCAATGGGGTAGTGGGAGATGGCTTGAAGAAGTACTGTGAGAGTTGGAGGATCAATGTTGAGGTCAACAACATTAGAGGGTTTGATGTGGTGCCTCAAGAATGTGTTGAGCACATAAAGAAATACATGACATCTTCTCAGTACAAAGCTGACTCAGAGAGGGCCTTGGAGGAAGTCACTCTCTACCTGAGCAGTAGTTGCTGTTGCACTTTGGAGGGTGATGGTAAAGATGCTTGGATTTTTGATGTTGATGATACACTCCTCTCCACTATTCCTTATTTCAAGAAACATGGTTTTGGGGGAGAGAAGTTGAATGCAACATCCTTGGAAACATGGATGAAAGAGAGCAAGGCACCAGCTCTAGAGCACACACTAAAGCTCTTCCATGATATCAAAGACAGAGGATTTAAGATCTTTCTCATCTCTTCAAGAAGGGAGACCCTTAGATCTCCAAGTGTAGATAACCTCATTAAGGTTGGATACCATGGATGGACTAATCTTGTGCTAAGGGGTCTTGAAGATGAATTCATGGAAGTGCAGAAGTACAAGTCTGAGGCAAGGCATCAACTCGTCAATGAAGGTTACCGCATTTGGGGCATAATAGGAGATCAGTGGAGCAGTTTCGAGGGACTTCCCATTGCGAAAAGAACATTCAAACTACCCAATTCAATGTACTATGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Acid_phosphat_B

Protein Analysis

318

Amino Acids

36.54

Weight (kDa)

7.03

Isoelectric Point (pI)

35.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Acid_phosphat_B PF03767 100 - 318 1.8e-77 HAD superfamily, subfamily IIIB (Acid phosphatase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 234
AccB1I GGYRCC 2 cut(s) 364, 625
AccI GTMKAC 1 cut(s) 149
AciI CCGC 1 cut(s) 859
AclWI GGATC 1 cut(s) 332
AcsI RAATTY 3 cut(s) 95, 133, 797
AfaI GTAC 4 cut(s) 308, 416, 818, 947
AfiI CCNNNNNNNGG 2 cut(s) 745, 865
AjnI CCWGG 1 cut(s) 101
AjuI GAANNNNNNNTTGG 2 cut(s) 124, 156
AluBI AGCT 3 cut(s) 422, 632, 652
AluI AGCT 3 cut(s) 422, 632, 652
Alw21I GWGCWC 2 cut(s) 387, 642
Alw26I GTCTC 2 cut(s) 167, 704
AlwI GGATC 1 cut(s) 332
AoxI GGCC 2 cut(s) 172, 435
ApoI RAATTY 3 cut(s) 95, 133, 797
AspLEI GCGC 1 cut(s) 28
AspS9I GGNCC 1 cut(s) 435
AsuHPI GGTGA 1 cut(s) 503
BanI GGYRCC 2 cut(s) 364, 625
Bbv12I GWGCWC 2 cut(s) 387, 642
BccI CCATC 3 cut(s) 287, 488, 756
BciT130I CCWGG 1 cut(s) 103
BciVI GTATCC 1 cut(s) 746
BcoDI GTCTC 2 cut(s) 167, 704
BfaI CTAG 3 cut(s) 42, 635, 955
BfuI GTATCC 1 cut(s) 746
BglII AGATCT 2 cut(s) 684, 719
BmcAI AGTACT 1 cut(s) 308
Bme1390I CCNGG 1 cut(s) 103
BmgT120I GGNCC 1 cut(s) 435
BmiI GGNNCC 2 cut(s) 366, 627
BmrFI CCNGG 1 cut(s) 103
BmsI GCATC 2 cut(s) 493, 844
Bpu10I CCTNAGC 2 cut(s) 461, 779
BpuEI CTTGAG 1 cut(s) 354
BsaI GGTCTC 1 cut(s) 704
BsaJI CCNNGG 4 cut(s) 102, 438, 597, 757
BsaWI WCCGGW 1 cut(s) 258
Bsc4I CCNNNNNNNGG 2 cut(s) 745, 865
Bse1I ACTGG 1 cut(s) 61
Bse3DI GCAATG 2 cut(s) 175, 909
BseBI CCWGG 1 cut(s) 103
BseDI CCNNGG 4 cut(s) 102, 438, 597, 757
BseGI GGATG 3 cut(s) 593, 615, 767
BseLI CCNNNNNNNGG 2 cut(s) 745, 865
BseMI GCAATG 2 cut(s) 175, 909
BseMII CTCAG 4 cut(s) 425, 441, 452, 816
BseNI ACTGG 1 cut(s) 61
BseRI GAGGAG 2 cut(s) 218, 524
BsgI GTGCAG 1 cut(s) 830
BshFI GGCC 2 cut(s) 174, 437
BshNI GGYRCC 2 cut(s) 364, 625
BsiHKAI GWGCWC 2 cut(s) 387, 642
BsiSI CCGG 1 cut(s) 259
BslFI GGGAC 1 cut(s) 915
BslI CCNNNNNNNGG 2 cut(s) 745, 865
BsmAI GTCTC 2 cut(s) 167, 704
BsmFI GGGAC 1 cut(s) 915
BsmI GAATGC 1 cut(s) 592
BsnI GGCC 2 cut(s) 174, 437
Bso31I GGTCTC 1 cut(s) 704
Bsp1286I GDGCHC 2 cut(s) 387, 642
Bsp143I GATC 4 cut(s) 324, 684, 719, 880
Bsp19I CCATGG 1 cut(s) 757
BspACI CCGC 1 cut(s) 859
BspANI GGCC 2 cut(s) 174, 437
BspCNI CTCAG 4 cut(s) 424, 440, 453, 817
BspLI GGNNCC 2 cut(s) 366, 627
BspPI GGATC 1 cut(s) 332
BspQI GCTCTTC 1 cut(s) 659
BspT107I GGYRCC 2 cut(s) 364, 625
BspTNI GGTCTC 1 cut(s) 704
BsrDI GCAATG 2 cut(s) 175, 909
BsrI ACTGG 1 cut(s) 61
BssECI CCNNGG 4 cut(s) 102, 438, 597, 757
BssMI GATC 4 cut(s) 324, 684, 719, 880
BssT1I CCWWGG 3 cut(s) 438, 597, 757
Bst2UI CCWGG 1 cut(s) 103
Bst4CI ACNGT 2 cut(s) 147, 311
Bst6I CTCTTC 2 cut(s) 659, 703
BstAPI GCANNNNNTGC 1 cut(s) 471
BstC8I GCNNGC 1 cut(s) 128
BstDEI CTNAG 6 cut(s) 411, 427, 461, 716, 779, 825
BstDSI CCRYGG 1 cut(s) 757
BstEII GGTNACC 1 cut(s) 854
BstENI CCTNNNNNAGG 1 cut(s) 743
BstF5I GGATG 3 cut(s) 593, 615, 767
BstHHI GCGC 1 cut(s) 28
BstKTI GATC 4 cut(s) 327, 687, 722, 883
BstMAI GTCTC 2 cut(s) 167, 704
BstMBI GATC 4 cut(s) 324, 684, 719, 880
BstMWI GCNNNNNNNGC 2 cut(s) 471, 867
BstNI CCWGG 1 cut(s) 103
BstNSI RCATGY 1 cut(s) 130
BstPI GGTNACC 1 cut(s) 854
BstSCI CCNGG 1 cut(s) 101
BstX2I RGATCY 2 cut(s) 684, 719
BstYI RGATCY 2 cut(s) 684, 719
BsuI GTATCC 1 cut(s) 746
BsuRI GGCC 2 cut(s) 174, 437
BtgI CCRYGG 1 cut(s) 757
BtsCI GGATG 3 cut(s) 593, 615, 767
BtsIMutI CAGTG 2 cut(s) 143, 890
Cac8I GCNNGC 1 cut(s) 128
CfoI GCGC 1 cut(s) 28
Cfr13I GGNCC 1 cut(s) 435
Csp6I GTAC 4 cut(s) 307, 415, 817, 946
CviAII CATG 8 cut(s) 85, 127, 400, 563, 606, 659, 758, 802
CviJI RGCY 7 cut(s) 116, 174, 297, 422, 437, 632, 652
CviKI_1 RGCY 7 cut(s) 116, 174, 297, 422, 437, 632, 652
CviQI GTAC 4 cut(s) 307, 415, 817, 946
DdeI CTNAG 6 cut(s) 411, 427, 461, 716, 779, 825
DpnI GATC 4 cut(s) 326, 686, 721, 882
DpnII GATC 4 cut(s) 324, 684, 719, 880
Eam1104I CTCTTC 2 cut(s) 659, 703
EarI CTCTTC 2 cut(s) 659, 703
Eco130I CCWWGG 3 cut(s) 438, 597, 757
Eco31I GGTCTC 1 cut(s) 704
Eco32I GATATC 1 cut(s) 664
Eco91I GGTNACC 1 cut(s) 854
EcoNI CCTNNNNNAGG 1 cut(s) 743
EcoO109I RGGNCCY 1 cut(s) 435
EcoO65I GGTNACC 1 cut(s) 854
EcoRI GAATTC 1 cut(s) 797
EcoRII CCWGG 1 cut(s) 101
EcoRV GATATC 1 cut(s) 664
EcoT14I CCWWGG 3 cut(s) 438, 597, 757
ErhI CCWWGG 3 cut(s) 438, 597, 757
FaeI CATG 8 cut(s) 88, 130, 403, 566, 609, 662, 761, 805
FaqI GGGAC 1 cut(s) 915
FatI CATG 8 cut(s) 84, 126, 399, 562, 605, 658, 757, 801
FblI GTMKAC 1 cut(s) 149
FokI GGATG 3 cut(s) 580, 622, 774
FspBI CTAG 3 cut(s) 42, 635, 955
GlaI GCGC 1 cut(s) 27
HaeIII GGCC 2 cut(s) 174, 437
HapII CCGG 1 cut(s) 259
HhaI GCGC 1 cut(s) 28
Hin1II CATG 8 cut(s) 88, 130, 403, 566, 609, 662, 761, 805
Hin6I GCGC 1 cut(s) 26
HinP1I GCGC 1 cut(s) 26
HincII GTYRAC 2 cut(s) 150, 340
HindII GTYRAC 2 cut(s) 150, 340
HinfI GANTC 1 cut(s) 425
HpaII CCGG 1 cut(s) 259
HphI GGTGA 1 cut(s) 503
Hpy166II GTNNAC 3 cut(s) 66, 150, 340
Hpy188I TCNGA 2 cut(s) 430, 826
Hpy188III TCNNGA 6 cut(s) 42, 371, 556, 635, 702, 788
Hpy8I GTNNAC 3 cut(s) 66, 150, 340
HpyAV CCTTC 3 cut(s) 16, 699, 845
HpyCH4III ACNGT 2 cut(s) 147, 311
HpyCH4V TGCA 6 cut(s) 88, 168, 239, 480, 590, 811
HpyF10VI GCNNNNNNNGC 2 cut(s) 471, 867
HpyF3I CTNAG 6 cut(s) 411, 427, 461, 716, 779, 825
Hsp92II CATG 8 cut(s) 88, 130, 403, 566, 609, 662, 761, 805
HspAI GCGC 1 cut(s) 26
Kzo9I GATC 4 cut(s) 324, 684, 719, 880
LguI GCTCTTC 1 cut(s) 659
LmnI GCTCC 3 cut(s) 205, 246, 888
LpnPI CCDG 7 cut(s) 23, 74, 88, 115, 272, 473, 642
LweI GCATC 2 cut(s) 493, 844
MaeI CTAG 3 cut(s) 42, 635, 955
MaeIII GTNAC 2 cut(s) 448, 854
MalI GATC 4 cut(s) 326, 686, 721, 882
MboI GATC 4 cut(s) 324, 684, 719, 880
MboII GAAGA 6 cut(s) 280, 313, 399, 646, 690, 803
MflI RGATCY 2 cut(s) 684, 719
MhlI GDGCHC 2 cut(s) 387, 642
MluCI AATT 4 cut(s) 95, 133, 797, 937
MlyI GAGTC 1 cut(s) 419
MmeI TCCRAC 2 cut(s) 299, 730
MseI TTAA 4 cut(s) 93, 180, 681, 744
MslI CAYNNNNRTG 1 cut(s) 806
MspI CCGG 1 cut(s) 259
MspR9I CCNGG 1 cut(s) 103
Mva1269I GAATGC 1 cut(s) 592
MvaI CCWGG 1 cut(s) 103
MwoI GCNNNNNNNGC 2 cut(s) 471, 867
NcoI CCATGG 1 cut(s) 757
NdeII GATC 4 cut(s) 324, 684, 719, 880
NlaIII CATG 8 cut(s) 88, 130, 403, 566, 609, 662, 761, 805
NlaIV GGNNCC 2 cut(s) 366, 627
NmuCI GTSAC 1 cut(s) 448
NspI RCATGY 1 cut(s) 130
PaeI GCATGC 1 cut(s) 130
PciSI GCTCTTC 1 cut(s) 659
PctI GAATGC 1 cut(s) 592
PleI GAGTC 1 cut(s) 419
PpsI GAGTC 1 cut(s) 419
PsiI TTATAA 1 cut(s) 234
Psp6I CCWGG 1 cut(s) 101
PspEI GGTNACC 1 cut(s) 854
PspGI CCWGG 1 cut(s) 101
PspN4I GGNNCC 2 cut(s) 366, 627
PspPI GGNCC 1 cut(s) 435
PsuI RGATCY 2 cut(s) 684, 719
RsaI GTAC 4 cut(s) 308, 416, 818, 947
RsaNI GTAC 4 cut(s) 307, 415, 817, 946
RseI CAYNNNNRTG 1 cut(s) 806
SalI GTCGAC 1 cut(s) 148
SapI GCTCTTC 1 cut(s) 659
SaqAI TTAA 4 cut(s) 93, 180, 681, 744
Sau3AI GATC 4 cut(s) 324, 684, 719, 880
Sau96I GGNCC 1 cut(s) 435
ScaI AGTACT 1 cut(s) 308
SchI GAGTC 1 cut(s) 419
ScrFI CCNGG 1 cut(s) 103
SduI GDGCHC 2 cut(s) 387, 642
SfaNI GCATC 2 cut(s) 493, 844
SmiMI CAYNNNNRTG 1 cut(s) 806
SmlI CTYRAG 1 cut(s) 369
SmoI CTYRAG 1 cut(s) 369
SphI GCATGC 1 cut(s) 130
Sse9I AATT 4 cut(s) 95, 133, 797, 937
SsiI CCGC 1 cut(s) 859
SspMI CTAG 3 cut(s) 42, 635, 955
StyD4I CCNGG 1 cut(s) 101
StyI CCWWGG 3 cut(s) 438, 597, 757
TaaI ACNGT 2 cut(s) 147, 311
TaqI TCGA 2 cut(s) 149, 897
TasI AATT 4 cut(s) 95, 133, 797, 937
TatI WGTACW 4 cut(s) 306, 414, 816, 945
Tru1I TTAA 4 cut(s) 93, 180, 681, 744
Tru9I TTAA 4 cut(s) 93, 180, 681, 744
TscAI CASTG 2 cut(s) 150, 890
TseFI GTSAC 1 cut(s) 448
Tsp45I GTSAC 1 cut(s) 448
TspDTI ATGAA 4 cut(s) 626, 790, 810, 864
TspRI CASTG 2 cut(s) 150, 890
XagI CCTNNNNNAGG 1 cut(s) 743
XapI RAATTY 3 cut(s) 95, 133, 797
XbaI TCTAGA 2 cut(s) 41, 634
XceI RCATGY 1 cut(s) 130
XcmI CCANNNNNNNNNTGG 1 cut(s) 283
XmiI GTMKAC 1 cut(s) 149
XspI CTAG 3 cut(s) 42, 635, 955
ZrmI AGTACT 1 cut(s) 308
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.